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bioelisa
READ HIGHLIGHTED CHANGES
bioelisa HAV IgM
3000-1098
96 tests
Procedural flow chart / Esquema del procedimiento / Schematischer Ablauf /
Schéma de la procédure / Schema del procedimento / Esquema do procedimento
Dilute Samples ( 1/200), DO NOT dilute Controls
Diluir Muestras (1/200), NO diluir los Controles
Proben verdünnen (1/200), DIE Kontrollen nicht verdünnen
Diluer Échantillons (1/200), NE PAS diluer les contrôles
Diluire Campioni (1/200), NON diluire i Controlli
Diluir Amostras (1/200), NÃO diluir os Controles

Pipette Samples Diluent: 100 µl/well
Dispensar Diluyente de las Muestras: 100 µl/pocillo
Probenverdünnungsmittel verteilen: 100 µl/Vertiefung
Dispenser Diluant des Échantillons: 100 µl/puits
Dispensare Diluente dei Campioni: 100 µl/pozzetto
Pipetar Diluente de Amostras: 100 µl/pocinho

Pipette pre-diluted Samples: 5 µl/well
Dispensar Muestras prediluidas: 5 µl/pocillo
Vorverdünnte Proben verteilen: 5 µl/Vertiefung
Dispenser Échantillons pré dilués: 5 µl/puits
Dispensare Campioni prediluiti: 5 µl/pozzetto
Pipetar Amostras pré-diluídas: 5 µl/pocinho

Pipette Controls: 100 µl/well
Dispensar Controles: 100 µl/pocillo
Kontrollen verteilen: 100 µl/Vertiefung
Dispenser Contrôles: 100 µl/puits
Dispensare Controlli: 100 µl/pozzetto
Pipetar Controles: 100 µl/pocinho

Incubate 1 hour at 37°C
Incubar 1 hora a 37°C
1 Stunde bei 37°C inkubieren
Incuber 1 heure à 37°C
Incubare 1 ora a 37°C
Incubar 1 hora a 37°C

Wash 6x / Lavar 6x
Waschen 6x / Laver 6x
Lavare 6x / Lavar 6x

Pipette Conjugate and Virus: 50 µl/well and 50 µl/well
Dispensar el Conjugado y el Virus: 50 µl/pocillo y 50 µl/pocillo
Konjugat und Virus pipettieren: 50 µl/Vertiefung und 50 µl/Vertiefung
Dispenser le Conjugué et le Virus: 50 µl/puits et 50 µl/puits
Dispensare il Coniugato e il Virus: 50 µl/pozzetto e 50 µl/pozzetto
Pipetar o Conjugado e o Vírus: 50 µl/pocinho e 50 µl/pocinho

Incubate 1 hour at 37°C
Incubar 1 hora a 37°C
1 Stunde bei 37°C inkubieren
Incuber 1 heure à 37°C
Incubare 1 ora a 37°C
Incubar 1 hora a 37°C

Wash 6x / Lavar 6x
Waschen 6x / Laver 6x
Lavare 6x / Lavar 6x

Pipette Substrate: 100 µl/well
Dispensar el Sustrato: 100 µl/pocillo
Substrat pipettieren: 100 µl/Vertiefung
Dispenser le Substrat: 100 µl/puits
Dispensare il Substrato: 100 µl/pozzetto
Pipetar o Substrato: 100 µl/pocinho

Incubate 30 minutes at RT
Incubar 30 minutos a TA
30 Minuten bei RT inkubieren
Incuber 30 minutes à TA
Incubare 30 minuti a TA
Incubar 30 minutos a TA

Pipette Stopping Solution: 100 µl/well
Dispensar la Solución de Parada: 100 µl/pocillo
Stopplösung zufügen: 100 µl/Vertiefung
Dispenser la Solution d’Arrêt: 100 µl/puits
Dispensare la Soluzione Bloccante: 100 µl/pozzetto
Pipetar a Solução de Bloqueio: 100 µl/pocinho

Cut-off: NCx + (PCx/4)
Valor umbral: CNx + (CPx/4)
Schwellenwert: NKx + (PKx/4)
Valeur seuil: CNx + (CPx/4)
Valore di soglia: CNx + (CPx/4)
Cut-off: CNx + (CPx/4)
Read at 450 nm
Leer a 450 nm
Bei 450 nm ablesen
Lire à 450 nm
Leggere a 450 nm
Ler a 450 nm
BIOKIT, S.A. - Can Malé s/n - 08186 Lliçà d’Amunt - Barcelona - SPAIN
3000-1098 R07 06.2012 procedural flow chart.doc
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