Multi-Omics approach for the analysis of Serum Biomolecules in Cardiovascular Diseases Introduction Jesús Lavado García, Celia Castañs García, Aleksandra Binek, Inmaculada Jorge Cerrudo, Alessia Ferrarini, Jesús Vázquez Cardiovascular Proteomics, CNIC. Melchor Fernández Almagro 3, 28029 Madrid, Spain Cardiovascular disease is the first-leading cause of death worldwide. Thus research on myocardial infarction represents an essential key point for the development of novel future therapies promoting the improvement of daily life. The use of the organism  Sus scrofa (common pig) has been proven highly useful in the study of heart conditions . Here a combined method of proteomics and metabolomics is applied to the analysis of pig’s serum in order to unveil molecules (proteins and metabolites) involved in ischemia-reperfusion affection. Extraction “If I can stop one heart from breaking, I shall not live in vain.” Emily Dickinson Fractionation Identification Post Translational Modification Analysis Quantification Conclusion This technology allows a fast, deep, reproducible and robust analysis of serum proteome and metabolome providing new insights to the study of heart infarction in pig model. It contributes to the discovery of novel molecules for diagnosis, prognosis and future therapies. This avant-garde approach grants applicability to different biological samples. Acknowledgements Thanks to CNIC Institute and its funding with ‘Beca Máster CNIC’. References  Kooij V. et al. (2014) PROTEOMICS - Clinical Applications Prot Clin Appl 8:653–664.  Whiley L. et al. (2012).Analytical Chemistry Anal Chem 84:5992–5999.  Chick JM. et al. (2015). Nat Biotechnol Nature Biotechnology 33:743– 749.  García-Marqués F. et al. (2016). Mol Cell Proteomics Molecular & Cellular Proteomics 15:1740–1760.