Anexos - Tesis

ANEXO 1
MATERIALES REQUERIDOS POR ENSAYOS REALIZADOS
ACTIVIDAD PROTEOLITICA
REACTIVOS
Cloruro de Sodio (NaCl)
Peso molecular = 58,44 g/mol
Marca: Merck
Ácido Tricloroacético (C2HCl3O2)
Peso molecular = 163,39 g/mol
Marca: Merck
Papel Whatman Nº1
Marca: Merck
Serum Albumina de Bovina.
Marca: SIGMA-ALDRICH.
Reactivo de Bradford.
Marca: SIGMA-ALDRICH.
EQUIPOS
Balanza Granataria
Marca: MFD by A&D, LTDA.
Modelo: EK-120A.
Fabricada en Japón.
Balanza Analítica
Marca: ADAM.
Modelo: WB 2000.
Fabricado en Alemania.
Espectrofotómetro.
Marca: Lambda
Modelo: 11 UV/VIS
Fabricado en Alemania.
Cubetas.
Marca: Starna.
Modelo: 9, Material:Q.
Fabricado en Alemania.
Micropipetas.
Marca: GILSON – PIPETMAN.
Capacidad: 1000, 200, 20 y 10 μL.
Fabricado en Francia.
INSUMOS
Bísturi
Gradillas
Embudos analíticos
Vasos precipitados
Tubos de ensayo.
Agua destilada.
Guantes de latex.
Piseta.
Hielo.
Pipeta volumetrica 3ml.
Tubos eppendorf.
GLUTATION PEROXIDASA
REACTIVOS
Cloruro de Sodio (NaCl)
Peso molecular = 58,44 g/mol
Marca: Merck
Trizma Base (C4H11NO3)
Peso molecular = 121,1 g/mol
Marca: SIGMA CHEMICAL CO.
Ácido clorhídrico (HCl)
Peso molecular = 36,46 g/mol.
Marca: Winkler, Ltda.
Glutatión Reductasa
168 unidades/mg de proteína
Marca: SIGMA - ALDRICH
Peróxido de Hidrógeno (H2O2)
Peso molecular = 34,01 g/mol.
Marca: FASA.
Glutatión Reducido (C10H17N3O6S)
Peso molecular = 307,33 g/mol
Marca: SIGMA - ALDRICH
EDTA
Peso molecular = 292,24 g/mol
Marca: SIGMA - ALDRICH
NADPH
Marca: SIGMA - ALDRICH
EQUIPOS
Balanza Analítica
Marca: ADAM.
Modelo: WB 2000.
Fabricado en Alemania.
Micropipetas.
Marca: GILSON – PIPETMAN.
Capacidad: 1000, 200, 20 y 10 μL.
Fabricado en Francia.
Espectrofotómetro.
Marca: Metertek
Modelo: SP - 830
Fabricado
Cubetas.
Marca: Starna.
Modelo: 9, Material:Q.
Fabricado en Alemania.
INSUMOS
Bísturi
Gradillas
Embudos analíticos
Vasos precipitados
Tubos de ensayo.
Guantes de latex.
Piseta.
Hielo.
Tubos eppendorf.
Agua destilada.
METALOPROTEASAS
REACTIVOS
Glicina (C2H5NO2)
Peso molecular = 75,07 g/mol.
Marca: SIGMA CHEMICAL CO.
Trizma Base (C4H11NO3)
Peso molecular = 121,1 g/mol
Marca: SIGMA CHEMICAL CO.
Temed (C6H16N2)
Peso molecular = 116,2 g/mol.
Marca: SIGMA CHEMICAL CO.
Acrilamida (C3H5NO)
Peso molecular = 71,08 g/mol.
Marca: SIGMA CHEMICAL CO.
Cloruro de calcio (CaCl2)
Peso molecular = 110,99 g/mol.
Marca: SIGMA-ALDRICH.
Ácido clorhídrico (HCl)
Peso molecular = 36,46 g/mol.
Marca: Winkler, Ltda.
Azul Brillante R. (C45H44N3O7S2Na)
Peso molecular = 826,0 g/mol.
Marca: SIGMA CHEMICAL CO.
Ácido acético (CH3COOH)
Peso molecular = 60,05 g/mol.
Marca: Merck.
Dodecil sulfato de amonio (SDS)
Peso molecular = 288,38 g/mol
Marca : J. T. Baker Inc.
Glicerol (C3H8O3)
Peso molecular= 92,09 g/mol
Marca: SIGMA CHEMICAL CO.
Persulfato de amonio (NH4)2S2O8
Peso molecular = 228,2 g/mol.
Marca: SIGMA CHEMICAL CO.
N,N’-metilen-bis-acrilamida(C7H10N2O2)
Peso molecular = 154,2 g/mol.
Marca: SIGMA CHEMICAL CO.
Azul de bromofenol(C19H9Br4O5SNa)
Peso molecular= 691,9 g/mol
Marca: SIGMA CHEMICAL CO.
Azida de sodio (NaN3)
Peso molecular = 65,01
Marca: SIGMA-ALDRICH.
Triton X - 100
Marca: SIGMA
Papel Wathman Nº1
Marca: Merck.
Gelatina
Marca: Gourmet.
Estándar De Masa Molecular.
Kaleidoscope Prestained Standards
Marca: BIO RAD
Catalog 161 - 0324
Ver Figura y Tabla.
EQUIPOS
Mini Protean III
Marca: BIO RAD
Micropipetas.
Marca: GILSON – PIPETMAN.
Capacidad: 1000, 200, 20 y 10 μL.
Fabricado en Francia.
Espectrofotómetro.
Marca: Metertek
Modelo: SP - 830
Cubetas.
Marca: Starna.
Modelo: 9, Material:Q.
Fabricado en Alemania.
Agitador orbital
Marca: TRILAB
Estufa
Marca: MEMMERT
INSUMOS
Bísturi
Gradillas
Embudos analíticos
Vasos precipitados
Tubos de ensayo.
Tabla: Kaleidoscope Prestained Standards
Figura: Kaleidoscope Prestained Standards
Guantes de latex.
Piseta.
Hielo.
Tubos eppendorf.
Agua destilada.
ANEXO 2
ESPECIFICACIONES DE LAS DIETAS SUMINISTRADAS A
LOS SALMONES EN ESTUDIO
™ Detalle de los antioxidantes adicionados a las distintas dietas de engorda
de salmones.
Tratamiento
Antioxidante adicionado
Harina
Aceite
Dieta I - Control
Etoxiquina /Tocoferoles
BHT/Tocoferoles
Dieta II
Extracto de Tocoferol
Tocoferoles libres
(Vitalox)
(Grindox TOCO 50)
Extracto de Tocoferol
Extracto de romero
(Vitalox)
(Guardian)
Dieta III
ANEXO 3
METODOLOGÍA ENSAYO ACTIVIDAD PROTEOLÍTICA
™ Determinación de la Actividad Proteolítica
La actividad enzimática se determinó utilizando el método expuesto por
Bradford en 1976.
El análisis se basa en la observación de que, el máximo de la absorbancia para
una solución ácida del azul brillante G-250 de Coomassie cambia desde 465 nm a 595
nm cuando se une a la proteína. Las interacciones hidrofóbicas e iónicas estabilizan la
forma aniónica del tinte, causando un cambio visible del color.
Algunas moléculas presentan afinidad por las proteínas y pueden fijarse a estas
de manera no covalente. El ensayo de Bradford se basa en la unión no covalente del
Azul de Coomassie a las proteínas.
Este compuesto presenta diferentes espectros de absorción según el estado de
protonación en el que se encuentra: la forma catiónica es de color rojo amarronado
(máximo de absorbancia a 470 nm), la neutra es verde (máximo a 650 nm) y la
aniónica es azul (máximo a 595 nm).
La presencia de proteínas favorece el desplazamiento del equilibrio hacia la
forma aniónica debido a la interacción de los grupos sulfónicos del Coomassie con los
grupos catiónicos las cadenas laterales de algunos residuos aminoacídicos de las
proteínas (principalmente Arg y Lys), produciéndose entonces la coloración azul que
puede medirse a λ = 595 nm.
Los pasos a seguir para la cuantificación de proteína mediante el Método
expuesto por Bradford (1976) fueron los siguientes:
1.- Elaboración de un estándar de BSA de concentración 1mg/ml.
Comprobar la concentración del éstandar leyendo su absorbancia a 280 nm.
El valor obtenido debe ser dividido por 0,63 y se obtendra la concentración exacta del
estándar.
2.- Crear una curva de calibración.
Preparar 5 tubos eppendorf con las siguientes cantidades de estándar de BSA y agua
destilada y agitar, dejar reposar por 5 minutos.
Tubo
Estándar de BSA (uL)
Agua (uL)
1
0
50
2
10
40
3
20
30
4
30
20
5
40
10
Luego adicionar 1500 µL de Reactivo de Bradford a cada uno de los tubos y agitar.
Dejar reposar por 10 minutos y leer en espectrofotómetro a 595 nm.
3.- Lectura de las muestras.
Se preparan tubos eppendorf con 40 µL del extracto obtenido de la muestra, se
adicionan 10 µL de agua destilada, se agita y se deja reposar por 5 minutos.
Luego adicionar 1500 µL de Reactivo de Bradford a cada uno de los tubos y agitar.
Dejar reposar por 10 minutos y leer en espectrofotómetro a 595 nm.
4.- Obtención de la proteína en la muestra.
Interpolar en la curva de calibración obtenida anteriormente, los valores de absorbancia
a 595 nm resultantes para las muestras.
ANEXO 4
METODOLOGÍA ENSAYO ACTIVIDAD PROTEOLÍTICA
ANALISIS ESTADÍSTICOS
™ Análisis estadísticos
Los análisis estadísticos se realizaron en el programa computacional
Statgraphics Plus versión 4.0, con un 95 % de confianza para todos los casos.
Los resultados de AP se obtuvieron en triplicado para cada uno de los
individuos analizados, por lo que primero se determinó la existencia o no de diferencias
estadísticamente significativas, mediante un análisis de ANOVA de dos vías, para las
replicas realizadas. Se realizó para cada una de los tiempos de almacenamiento y
dieta de engorda suministrada de forma independiente. Se determinó que éstas son
promediables.
Luego, con el promedio de estas réplicas, se determinó la existencia o no de
diferencias estadísticamente significativas, mediante un análisis de ANOVA de dos
vías, entre los individuos. Estos análisis se realizaron para las muestras provenientes
de individuos alimentados con las tres dietas analizadas de forma independiente. Se
determinó que los individuos son promediables.
Además, se realizó un análisis estadístico (ANOVA
de una vía), para
determinar si existe variación estadísticamente significativa en el comportamiento de la
AP durante el tiempo de almacenamiento, para las muestras provenientes de
individuos alimentados con las tres dietas analizadas de forma independiente. Además
se realizó correlación de Pearson, en el programa computacional antes mencionado,
estos análisis se realizaron en cada una de las dietas por separado. Las propiedades
físicas y sensoriales que se correlacionaron fueron las siguientes:
- Unidades formadoras de colonias
- Unidades formadoras de colonias / Promedio de RAM
- Cohesividad (Cizalla en músculo de Salmón coho cocido)
- Cohesividad (Cizalla en músculo de Salmón coho crudo)
- Cohesividad (Compresión)
- Color
- Color Roche descongelado.
- Deshidratación descongelado.
- Dureza (Newton) (Cizalla en músculo de Salmón coho cocido)
- Dureza (Newton) (Cizalla en músculo de Salmón coho crudo)
- Dureza (Newton) (Compresión)
- Elasticidad en salmón descongelado.
- Gaping.
- Goteo en el salmón descongelado.
- Olor putrido de salmón descongelado.
- Olor rancio.
- Olor rancio de salmón descongelado.
- Olor típico.
- Olor típico a salmón descongelado.
- pH.
- Porcentaje de agua perdida (Humedad).
- Porcentaje de agua pérdida (CRA).
- Porcentaje de agua perdida (Driping de músculo de salmón cocido).
- Porcentaje de líquido perdido (Driping de músculo de salmón crudo).
- Sabor oxidado.
- Sabor putrido.
- Sabor Rancio.
- Sabor típico.
- Tiempo.
Se realizó también un análisis en conjunto, ANOVA de una vía, para AP de las
muestras provenientes de individuos alimentados con las tres dietas, determinándose
si existían o no diferencias estadísticamente significativas tanto entre ellas, como en su
comportamiento en el tiempo.
ANEXO 5
METODOLOGÍA ENSAYO GLUTATION PEROXIDASA
ANALISIS ESTADÍSTICOS
™ Análisis estadísticos
Los análisis estadísticos se realizaron en el programa computacional
Statgraphics Plus versión 4.0, con un 95 % de confianza para todos los casos.
En una primera etapa, se realizó análisis preliminares para el montaje de la
técnica, donde se efectuaron los siguientes análisis estadísticos:
- ANOVA de dos vías para determinar el comportamiento de GSH – Px frente a la
cantidad de H2O2 adicionado.
- ANOVA de dos vías para determinar el comportamiento de GSH – Px frente a la
cantidad de GRd adicionada.
- ANOVA de dos vías para determinar el comportamiento de GSH – Px frente a la
cantidad de muestra adicionada.
Los resultados de GSH – Px se obtuvieron para cada individuo analizado, por lo
que primero se determinó la existencia o no de diferencias estadísticamente
significativas, mediante un análisis de ANOVA de dos vías, entre los individuos. Estos
análisis se realizaron para las muestras provenientes de individuos alimentados con las
tres dietas analizadas de forma independiente. Se determinó que los individuos son
promediables.
Además, se realizó un análisis estadístico (ANOVA
de una vía), para
determinar si existe variación estadísticamente significativa en el comportamiento de la
GSH – Px durante el tiempo de almacenamiento, para las muestras provenientes de
individuos alimentados con las tres dietas analizadas de forma independiente. Además
se realizó correlación de Pearson, en el programa computacional antes mencionado,
estos análisis se realizaron en cada una de las dietas por separado. Las propiedades
físicas y sensoriales que se correlacionaron fueron las siguientes:
- Unidades formadoras de colonias
- Unidades formadoras de colonias / Promedio de RAM
- Cohesividad (Cizalla en músculo de Salmón coho cocido)
- Cohesividad (Cizalla en músculo de Salmón coho crudo)
- Cohesividad (Compresión)
- Color
- Color Roche descongelado.
- Deshidratación descongelado.
- Dureza (Newton) (Cizalla en músculo de Salmón coho cocido)
- Dureza (Newton) (Cizalla en músculo de Salmón coho crudo)
- Dureza (Newton) (Compresión)
- Elasticidad en salmón descongelado.
- Gaping.
- Goteo en el salmón descongelado.
- Olor putrido de salmón descongelado.
- Olor rancio.
- Olor rancio de salmón descongelado.
- Olor típico.
- Olor típico a salmón descongelado.
- pH.
- Porcentaje de agua perdida (Humedad).
- Porcentaje de agua pérdida (CRA).
- Porcentaje de agua perdida (Driping de músculo de salmón cocido).
- Porcentaje de líquido perdido (Driping de músculo de salmón crudo).
- Sabor oxidado.
- Sabor putrido.
- Sabor Rancio.
- Sabor típico.
- Tiempo.
Se realizó una segunda correlación de Pearson, en el programa computacional
antes mencionado, estos análisis se realizaron en cada una de las dietas por separado.
Los indicadores que se correlacionaron fueron los siguientes:
- Dimetilamina (DMA).
- Formaldehído (HCHO).
- Índice de peróxidos.
- Indice de polienos.
- Nitrógeno básico volátil total (NBVT).
- Valor de p - anisidina.
- Tiempo.
Se realizó también un análisis en conjunto, ANOVA de una vía, para GSH - Px
de las muestras provenientes de individuos alimentados con las tres dietas,
determinándose si existían o no diferencias estadísticamente significativas tanto entre
ellas, como en su comportamiento en el tiempo.
ANEXO 6
METODOLOGÍA ENSAYO METALOPROTEINASAS
ANALISIS ESTADÍSTICOS
™ Análisis estadisticos
Los geles con actividad gelatinásica se introdujeron en el programa
computacional UN SCAN IT, el cual determina los pixeles en las bandas blancas, es
decir las que presentaron actividad enzimática. Los parámetros entregados por el
programa, para cada una de las bandas, son los siguientes:
- Píxel total.
- Background.
- Pix total.
- Pix Total (%).
- Y Centroid.
- Seg Area.
- Seg Area (%).
- Max pixel.
- Avg píxel.
Mediante el uso del programa computacional Statgraphics Plus versión 4.0, se
realizó un tratamiento estadístico de los datos, ANOVA multifactorial, para determinar
el comportamiento de las MMP’s con respecto al tiempo y la existencia o no de
diferencias estadísticamente significativas, con respecto al tiempo de almacenamiento
como a las dietas de engorda en estudio. Además se realizó correlación de Pearson,
en el programa computacional antes mencionado, estos análisis se realizaron en cada
una de las dietas por separado. Las propiedades físicas y sensoriales que se
correlacionaron fueron las siguientes:
- Unidades formadoras de colonias
- Unidades formadoras de colonias / Promedio de RAM
- Cohesividad (Cizalla en músculo de Salmón coho cocido)
- Cohesividad (Cizalla en músculo de Salmón coho crudo)
- Cohesividad (Compresión)
- Color
- Color Roche descongelado.
- Deshidratación descongelado.
- Dureza (Newton) (Cizalla en músculo de Salmón coho cocido)
- Dureza (Newton) (Cizalla en músculo de Salmón coho crudo)
- Dureza (Newton) (Compresión)
- Elasticidad en salmón descongelado.
- Gaping.
- Goteo en el salmón descongelado.
- Olor putrido de salmón descongelado.
- Olor rancio.
- Olor rancio de salmón descongelado.
- Olor típico.
- Olor típico a salmón descongelado.
- pH.
- Porcentaje de agua perdida (Humedad).
- Porcentaje de agua pérdida (CRA).
- Porcentaje de agua perdida (Driping de músculo de salmón cocido).
- Porcentaje de líquido perdido (Driping de músculo de salmón crudo).
- Sabor oxidado.
- Sabor putrido.
- Sabor Rancio.
- Sabor típico.
- Tiempo.
Se realizó una segunda correlación de Pearson, en el mismo programa
computacional. Los indicadores que se correlacionaron fueron los siguientes:
- AP.
- GSH – Px.
- MMP’s.
- Peso de los individuos.
ANEXO 7
ACTIVIDAD PROTEOLITICA PARA MUSCULO DE SALMON COHO PROVENIENTE
DE INDIVIDUOS ALIMENTADOS CON LAS DIETAS ANALIZADAS
DIETA I O CONTROL
Individuo
A
A
A
B
B
B
C
C
C
D
D
D
E
E
E
Réplica
1
2
3
1
2
3
1
2
3
1
2
3
1
2
3
mes 0
15.53
41.01
0.00
10.29
10.29
0.00
13.17
9.41
0.00
0.00
0.00
0.00
0.00
0.00
0.00
mes 3
0.00
0.00
0.00
10.08
1.95
1.87
12.69
2.28
14.46
4.35
0.00
0.00
0.00
0.00
0.00
mes 6
24.36
45.30
23.54
30.88
14.21
23.74
25.93
30.70
26.79
10.87
13.33
3.27
0.00
25.84
31.37
mes 9
0.00
0.00
0.00
14.60
22.63
19.18
24.40
34.00
34.45
8.90
0.00
0.00
3.91
30.33
17.68
mes 12
6.10
16.75
17.88
0.00
13.85
0.00
0.00
0.00
0.00
9.16
3.98
4.99
5.16
0.00
0.00
mes 18
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
-
DIETA II (EXCESO DE TOCOFEROLES)
Individuo
A
A
A
B
B
B
C
C
C
D
D
D
E
E
E
Réplica
1
2
3
1
2
3
1
2
3
1
2
3
1
2
3
mes 0
27.09
25.41
29.55
4.00
4.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
4.65
102.06
109.22
mes 3
0.00
24.39
9.38
0.00
1.66
6.36
8.13
0.00
16.80
4.88
29.84
6.76
0.00
0.00
0.00
mes 6
0.00
30.92
2.08
26.15
31.18
50.93
0.00
0.00
0.00
33.48
21.08
0.14
25.36
46.26
38.89
mes 9
0.00
0.00
3.98
4.45
30.52
4.54
3.25
8.18
4.97
0.00
0.00
0.00
9.87
6.08
7.67
mes 12
17.45
29.04
0.32
11.27
13.16
7.31
14.14
18.30
0.00
3.80
1.98
12.96
2.09
6.18
2.09
mes 18
11.30
6.53
0.00
0.00
12.68
0.00
14.93
12.55
17.96
21.57
27.53
33.89
-
mes 12
0.00
0.00
0.00
8.11
6.10
17.82
0.00
1.61
7.86
0.00
0.00
0.00
0.00
0.00
0.00
mes 18
0.00
0.00
0.00
0.00
0.00
0.00
17.93
16.34
31.94
46.15
44.00
56.88
-
DIETA III (EXTRACTO DE ROMERO)
Individuo
A
A
A
B
B
B
C
C
C
D
D
D
E
E
E
Réplica
1
2
3
1
2
3
1
2
3
1
2
3
1
2
3
mes 0
0.00
0.00
0.00
18.13
2.26
32.92
0.00
0.00
0.00
22.99
27.00
19.17
0.00
0.00
3.37
mes 3
0.00
23.73
9.75
0.00
0.00
0.00
0.00
0.00
0.00
41.57
25.19
11.76
0.00
17.47
0.00
mes 6
26.44
30.29
39.97
0.00
0.00
0.00
23.85
12.24
21.87
5.02
0.00
0.00
0.00
0.00
32.10
mes 9
941.26
714.16
742.93
816.64
574.15
651.17
293.16
275.72
284.40
255.44
202.04
174.08
1014.86
240.61
342.90
ANEXO 8
ANALISIS ESTADISTICOS – ANOVA DE DOS VIAS PARA DETERMINAR
DIFERENCIAS ESTADISTICAS ENTRE REPLICAS
A.- DIETA I O CONTROL
Mes 0 de almacenamiento
Analysis of Variance for ACTIVIDAD PROTEOLITICA - Type III Sums of Squares
-------------------------------------------------------------------------------Source
Sum of Squares
Df
Mean Square
F-Ratio
P-Value
-------------------------------------------------------------------------------MAIN EFFECTS
A:INDIVIDUO
714.049
4
178.512
2.23
0.1559
B:REPLICA
378.512
2
189.256
2.36
0.1565
RESIDUAL
641.533
8
80.1916
-------------------------------------------------------------------------------TOTAL (CORRECTED)
1734.09
14
-------------------------------------------------------------------------------All F-ratios are based on the residual mean square error.
ACTIVIDAD PROTEOLITICA
Multiple Range Tests for ACTIVIDAD PROTEOLITICA by REPLICA
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
REPLICA
Count
LS Mean
Homogeneous Groups
-------------------------------------------------------------------------------3
5
0.0
X
1
5
7.798
X
2
5
12.142
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------1 - 2
-4.344
16.1557
1 - 3
7.798
16.1557
2 - 3
12.142
16.1557
-------------------------------------------------------------------------------* denotes a statistically significant difference.
Scatterplot by Level Code
50
40
30
20
10
0
1
2
REPLICA
3
Mes 3 de almacenamiento
Analysis of Variance for ACTIVIDAD PROTEOLITICA - Type III Sums of Squares
-------------------------------------------------------------------------------Source
Sum of Squares
Df
Mean Square
F-Ratio
P-Value
-------------------------------------------------------------------------------MAIN EFFECTS
A:INDIVIDUO
207.86
4
51.9651
4.55
0.0328
B:REPLICA
52.4524
2
26.2262
2.30
0.1627
RESIDUAL
91.2829
8
11.4104
-------------------------------------------------------------------------------TOTAL (CORRECTED)
351.596
14
-------------------------------------------------------------------------------All F-ratios are based on the residual mean square error.
ACTIVIDAD PROTEOLITICA
Multiple Range Tests for ACTIVIDAD PROTEOLITICA by REPLICA
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
REPLICA
Count
LS Mean
Homogeneous Groups
-------------------------------------------------------------------------------2
5
0.846
X
3
5
3.266
X
1
5
5.424
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------1 - 2
4.578
6.09412
1 - 3
2.158
6.09412
2 - 3
-2.42
6.09412
-------------------------------------------------------------------------------* denotes a statistically significant difference.
Scatterplot by Level Code
15
12
9
6
3
0
1
2
REPLICA
3
Mes 6 de almacenamiento
Analysis of Variance for ACTIVIDAD PROTEOLITICA - Type III Sums of Squares
-------------------------------------------------------------------------------Source
Sum of Squares
Df
Mean Square
F-Ratio
P-Value
-------------------------------------------------------------------------------MAIN EFFECTS
A:INDIVIDUO
871.042
4
217.761
1.87
0.2097
B:REPLICA
139.961
2
69.9804
0.60
0.5717
RESIDUAL
932.873
8
116.609
-------------------------------------------------------------------------------TOTAL (CORRECTED)
1943.88
14
-------------------------------------------------------------------------------All F-ratios are based on the residual mean square error.
ACTIVIDAD PROTEOLITICA
Multiple Range Tests for ACTIVIDAD PROTEOLITICA by REPLICA
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
REPLICA
Count
LS Mean
Homogeneous Groups
-------------------------------------------------------------------------------1
5
18.408
X
3
5
21.742
X
2
5
25.876
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------1 - 2
-7.468
19.4817
1 - 3
-3.334
19.4817
2 - 3
4.134
19.4817
-------------------------------------------------------------------------------* denotes a statistically significant difference.
Scatterplot by Level Code
50
40
30
20
10
0
1
2
REPLICA
3
Mes 9 de almacenamiento
Analysis of Variance for ACTIVIDAD PROTEOLITICA - Type III Sums of Squares
-------------------------------------------------------------------------------Source
Sum of Squares
Df
Mean Square
F-Ratio
P-Value
-------------------------------------------------------------------------------MAIN EFFECTS
A:INDIVIDUO
1917.13
4
479.282
10.23
0.0031
B:REPLICA
124.046
2
62.0232
1.32
0.3187
RESIDUAL
374.886
8
46.8607
-------------------------------------------------------------------------------TOTAL (CORRECTED)
2416.06
14
-------------------------------------------------------------------------------All F-ratios are based on the residual mean square error.
ACTIVIDAD PROTEOLITICA
Multiple Range Tests for ACTIVIDAD PROTEOLITICA by REPLICA
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
REPLICA
Count
LS Mean
Homogeneous Groups
-------------------------------------------------------------------------------1
5
10.362
X
3
5
14.262
X
2
5
17.392
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------1 - 2
-7.03
12.35
1 - 3
-3.9
12.35
2 - 3
3.13
12.35
-------------------------------------------------------------------------------* denotes a statistically significant difference.
Scatterplot by Level Code
40
30
20
10
0
1
2
REPLICA
3
Mes 12 de almacenamiento
Analysis of Variance for ACTIVIDAD PROTEOLITICA - Type III Sums of Squares
-------------------------------------------------------------------------------Source
Sum of Squares
Df
Mean Square
F-Ratio
P-Value
-------------------------------------------------------------------------------MAIN EFFECTS
A:INDIVIDUO
331.11
4
82.7775
2.98
0.0883
B:REPLICA
22.9088
2
11.4544
0.41
0.6755
RESIDUAL
222.293
8
27.7866
-------------------------------------------------------------------------------TOTAL (CORRECTED)
576.312
14
-------------------------------------------------------------------------------All F-ratios are based on the residual mean square error.
ACTIVIDAD PROTEOLITICA
Multiple Range Tests for ACTIVIDAD PROTEOLITICA by REPLICA
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
REPLICA
Count
LS Mean
Homogeneous Groups
-------------------------------------------------------------------------------1
5
4.084
X
3
5
4.574
X
2
5
6.916
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------1 - 2
-2.832
9.50998
1 - 3
-0.49
9.50998
2 - 3
2.342
9.50998
-------------------------------------------------------------------------------* denotes a statistically significant difference.
Scatterplot by Level Code
18
15
12
9
6
3
0
1
2
REPLICA
Mes 18 de almacenamiento
Cannot perform analysis.
Data values are all equal.
3
B.- DIETA II (EXCESO DE TOCOFEROLES)
Mes 0 de almacenamiento
Analysis of Variance for ACTIVIDAD PROTEOLITICA - Type III Sums of Squares
-------------------------------------------------------------------------------Source
Sum of Squares
Df
Mean Square
F-Ratio
P-Value
-------------------------------------------------------------------------------MAIN EFFECTS
A:INDIVIDUO
11565.7
4
2891.43
4.19
0.0404
B:REPLICA
1322.18
2
661.09
0.96
0.4237
RESIDUAL
5522.11
8
690.264
-------------------------------------------------------------------------------TOTAL (CORRECTED)
18410.0
14
-------------------------------------------------------------------------------All F-ratios are based on the residual mean square error.
ACTIVIDAD PROTEOLITICA
Multiple Range Tests for ACTIVIDAD PROTEOLITICA by REPLICA
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
REPLICA
Count
LS Mean
Homogeneous Groups
-------------------------------------------------------------------------------1
5
7.148
X
2
5
26.294
X
3
5
27.754
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------1 - 2
-19.146
47.399
1 - 3
-20.606
47.399
2 - 3
-1.46
47.399
-------------------------------------------------------------------------------* denotes a statistically significant difference.
Scatterplot by Level Code
120
100
80
60
40
20
0
1
2
REPLICA
3
Mes 3 de almacenamiento
Analysis of Variance for ACTIVIDAD PROTEOLITICA - Type III Sums of Squares
-------------------------------------------------------------------------------Source
Sum of Squares
Df
Mean Square
F-Ratio
P-Value
-------------------------------------------------------------------------------MAIN EFFECTS
A:INDIVIDUO
401.794
4
100.448
1.21
0.3785
B:REPLICA
187.006
2
93.5029
1.12
0.3711
RESIDUAL
665.054
8
83.1318
-------------------------------------------------------------------------------TOTAL (CORRECTED)
1253.85
14
-------------------------------------------------------------------------------All F-ratios are based on the residual mean square error.
ACTIVIDAD PROTEOLITICA
Multiple Range Tests for ACTIVIDAD PROTEOLITICA by REPLICA
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
REPLICA
Count
LS Mean
Homogeneous Groups
-------------------------------------------------------------------------------1
5
2.602
X
3
5
7.86
X
2
5
11.178
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------1 - 2
-8.576
16.4492
1 - 3
-5.258
16.4492
2 - 3
3.318
16.4492
-------------------------------------------------------------------------------* denotes a statistically significant difference.
Scatterplot by Level Code
30
25
20
15
10
5
0
1
2
REPLICA
3
Mes 6 de almacenamiento
Analysis of Variance for ACTIVIDAD PROTEOLITICA - Type III Sums of Squares
-------------------------------------------------------------------------------Source
Sum of Squares
Df
Mean Square
F-Ratio
P-Value
-------------------------------------------------------------------------------MAIN EFFECTS
A:INDIVIDUO
3076.34
4
769.084
4.09
0.0429
B:REPLICA
228.284
2
114.142
0.61
0.5684
RESIDUAL
1504.89
8
188.111
-------------------------------------------------------------------------------TOTAL (CORRECTED)
4809.51
14
-------------------------------------------------------------------------------All F-ratios are based on the residual mean square error.
ACTIVIDAD PROTEOLITICA
Multiple Range Tests for ACTIVIDAD PROTEOLITICA by REPLICA
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
REPLICA
Count
LS Mean
Homogeneous Groups
-------------------------------------------------------------------------------1
5
16.998
X
3
5
18.408
X
2
5
25.888
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------1 - 2
-8.89
24.7439
1 - 3
-1.41
24.7439
2 - 3
7.48
24.7439
-------------------------------------------------------------------------------* denotes a statistically significant difference.
Scatterplot by Level Code
60
50
40
30
20
10
0
1
2
REPLICA
3
Mes 9 de almacenamiento
Analysis of Variance for ACTIVIDAD PROTEOLITICA - Type III Sums of Squares
-------------------------------------------------------------------------------Source
Sum of Squares
Df
Mean Square
F-Ratio
P-Value
-------------------------------------------------------------------------------MAIN EFFECTS
A:INDIVIDUO
336.32
4
84.0801
1.71
0.2412
B:REPLICA
87.4118
2
43.7059
0.89
0.4490
RESIDUAL
394.453
8
49.3066
-------------------------------------------------------------------------------TOTAL (CORRECTED)
818.185
14
-------------------------------------------------------------------------------All F-ratios are based on the residual mean square error.
ACTIVIDAD PROTEOLITICA
Multiple Range Tests for ACTIVIDAD PROTEOLITICA by REPLICA
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
REPLICA
Count
LS Mean
Homogeneous Groups
-------------------------------------------------------------------------------1
5
3.514
X
3
5
4.232
X
2
5
8.956
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------1 - 2
-5.442
12.6682
1 - 3
-0.718
12.6682
2 - 3
4.724
12.6682
-------------------------------------------------------------------------------* denotes a statistically significant difference.
Scatterplot by Level Code
40
30
20
10
0
1
2
REPLICA
3
Mes 12 de almacenamiento
Analysis of Variance for ACTIVIDAD PROTEOLITICA - Type III Sums of Squares
-------------------------------------------------------------------------------Source
Sum of Squares
Df
Mean Square
F-Ratio
P-Value
-------------------------------------------------------------------------------MAIN EFFECTS
A:INDIVIDUO
261.478
4
65.3694
1.07
0.4298
B:REPLICA
212.681
2
106.34
1.75
0.2348
RESIDUAL
487.136
8
60.8919
-------------------------------------------------------------------------------TOTAL (CORRECTED)
961.294
14
-------------------------------------------------------------------------------All F-ratios are based on the residual mean square error.
ACTIVIDAD PROTEOLITICA
Multiple Range Tests for ACTIVIDAD PROTEOLITICA by REPLICA
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
REPLICA
Count
LS Mean
Homogeneous Groups
-------------------------------------------------------------------------------3
5
4.536
X
1
5
9.75
X
2
5
13.732
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------1 - 2
-3.982
14.078
1 - 3
5.214
14.078
2 - 3
9.196
14.078
-------------------------------------------------------------------------------* denotes a statistically significant difference.
Scatterplot by Level Code
30
25
20
15
10
5
0
1
2
REPLICA
3
Mes 18 de almacenamiento
Analysis of Variance for ACTIVIDAD PROTEOLITICA - Type III Sums of Squares
-------------------------------------------------------------------------------Source
Sum of Squares
Df
Mean Square
F-Ratio
P-Value
-------------------------------------------------------------------------------MAIN EFFECTS
A:INDIVIDUO
1038.45
3
346.149
8.47
0.0141
B:REPLICA
16.9813
2
8.49067
0.21
0.8180
RESIDUAL
245.191
6
40.8651
-------------------------------------------------------------------------------TOTAL (CORRECTED)
1300.62
11
-------------------------------------------------------------------------------All F-ratios are based on the residual mean square error.
ACTIVIDAD PROTEOLITICA
Multiple Range Tests for ACTIVIDAD PROTEOLITICA by REPLICA
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
REPLICA
Count
LS Mean
Homogeneous Groups
-------------------------------------------------------------------------------1
4
11.95
X
3
4
12.9625
X
2
4
14.8225
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------1 - 2
-2.8725
13.8694
1 - 3
-1.0125
13.8694
2 - 3
1.86
13.8694
-------------------------------------------------------------------------------* denotes a statistically significant difference.
Scatterplot by Level Code
40
30
20
10
0
1
2
REPLICA
3
C.- DIETA III (EXTRACTO DE ROMERO)
Mes 0 de almacenamiento
Analysis of Variance for ACTIVIDAD PROTEOLITICA - Type III Sums of Squares
-------------------------------------------------------------------------------Source
Sum of Squares
Df
Mean Square
F-Ratio
P-Value
-------------------------------------------------------------------------------MAIN EFFECTS
A:INDIVIDUO
1489.76
4
372.44
6.78
0.0110
B:REPLICA
68.849
2
34.4245
0.63
0.5588
RESIDUAL
439.595
8
54.9494
-------------------------------------------------------------------------------TOTAL (CORRECTED)
1998.2
14
-------------------------------------------------------------------------------All F-ratios are based on the residual mean square error.
ACTIVIDAD PROTEOLITICA
Multiple Range Tests for ACTIVIDAD PROTEOLITICA by REPLICA
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
REPLICA
Count
LS Mean
Homogeneous Groups
-------------------------------------------------------------------------------2
5
5.852
X
1
5
8.224
X
3
5
11.092
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------1 - 2
2.372
13.3734
1 - 3
-2.868
13.3734
2 - 3
-5.24
13.3734
-------------------------------------------------------------------------------* denotes a statistically significant difference.
Scatterplot by Level Code
40
30
20
10
0
1
2
REPLICA
3
Mes 3 de almacenamiento
Analysis of Variance for ACTIVIDAD PROTEOLITICA - Type III Sums of Squares
-------------------------------------------------------------------------------Source
Sum of Squares
Df
Mean Square
F-Ratio
P-Value
-------------------------------------------------------------------------------MAIN EFFECTS
A:INDIVIDUO
1413.0
4
353.25
3.86
0.0492
B:REPLICA
202.177
2
101.088
1.11
0.3768
RESIDUAL
731.598
8
91.4497
-------------------------------------------------------------------------------TOTAL (CORRECTED)
2346.78
14
-------------------------------------------------------------------------------All F-ratios are based on the residual mean square error.
ACTIVIDAD PROTEOLITICA
Multiple Range Tests for ACTIVIDAD PROTEOLITICA by REPLICA
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
REPLICA
Count
LS Mean
Homogeneous Groups
-------------------------------------------------------------------------------3
5
4.302
X
1
5
8.314
X
2
5
13.278
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------1 - 2
-4.964
17.2525
1 - 3
4.012
17.2525
2 - 3
8.976
17.2525
-------------------------------------------------------------------------------* denotes a statistically significant difference.
Scatterplot by Level Code
50
40
30
20
10
0
1
2
REPLICA
3
Mes 6 de almacenamiento
Analysis of Variance for ACTIVIDAD PROTEOLITICA - Type III Sums of Squares
-------------------------------------------------------------------------------Source
Sum of Squares
Df
Mean Square
F-Ratio
P-Value
-------------------------------------------------------------------------------MAIN EFFECTS
A:INDIVIDUO
2136.65
4
534.162
7.22
0.0091
B:REPLICA
286.573
2
143.286
1.94
0.2059
RESIDUAL
591.512
8
73.9391
-------------------------------------------------------------------------------TOTAL (CORRECTED)
3014.73
14
-------------------------------------------------------------------------------All F-ratios are based on the residual mean square error.
ACTIVIDAD PROTEOLITICA
Multiple Range Tests for ACTIVIDAD PROTEOLITICA by REPLICA
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
REPLICA
Count
LS Mean
Homogeneous Groups
-------------------------------------------------------------------------------2
5
8.506
X
1
5
11.062
X
3
5
18.788
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------1 - 2
2.556
15.5131
1 - 3
-7.726
15.5131
2 - 3
-10.282
15.5131
-------------------------------------------------------------------------------* denotes a statistically significant difference.
Scatterplot by Level Code
40
30
20
10
0
1
2
REPLICA
3
Mes 9 de almacenamiento
Analysis of Variance for ACTIVIDAD PROTEOLITICA - Type III Sums of Squares
-------------------------------------------------------------------------------Source
Sum of Squares
Df
Mean Square
F-Ratio
P-Value
-------------------------------------------------------------------------------MAIN EFFECTS
A:INDIVIDUO
760918.0
4
190230.0
7.03
0.0099
B:REPLICA
202109.0
2
101054.0
3.73
0.0716
RESIDUAL
216563.0
8
27070.4
-------------------------------------------------------------------------------TOTAL (CORRECTED)
1.17959E6
14
-------------------------------------------------------------------------------All F-ratios are based on the residual mean square error.
ACTIVIDAD PROTEOLITICA
Multiple Range Tests for ACTIVIDAD PROTEOLITICA by REPLICA
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
REPLICA
Count
LS Mean
Homogeneous Groups
-------------------------------------------------------------------------------2
5
401.336
X
3
5
439.096
X
1
5
664.272
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------1 - 2
262.936
296.831
1 - 3
225.176
296.831
2 - 3
-37.76
296.831
-------------------------------------------------------------------------------* denotes a statistically significant difference.
Scatterplot by Level Code
1200
1000
800
600
400
200
0
1
2
REPLICA
3
Mes 12 de almacenamiento
Analysis of Variance for ACTIVIDAD PROTEOLITICA - Type III Sums of Squares
-------------------------------------------------------------------------------Source
Sum of Squares
Df
Mean Square
F-Ratio
P-Value
-------------------------------------------------------------------------------MAIN EFFECTS
A:INDIVIDUO
257.051
4
64.2627
7.25
0.0090
B:REPLICA
42.1191
2
21.0595
2.38
0.1549
RESIDUAL
70.9199
8
8.86498
-------------------------------------------------------------------------------TOTAL (CORRECTED)
370.09
14
-------------------------------------------------------------------------------All F-ratios are based on the residual mean square error.
ACTIVIDAD PROTEOLITICA
Multiple Range Tests for ACTIVIDAD PROTEOLITICA by REPLICA
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
REPLICA
Count
LS Mean
Homogeneous Groups
-------------------------------------------------------------------------------2
5
1.542
X
1
5
1.622
X
3
5
5.136
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------1 - 2
0.08
5.37156
1 - 3
-3.514
5.37156
2 - 3
-3.594
5.37156
-------------------------------------------------------------------------------* denotes a statistically significant difference.
Scatterplot by Level Code
18
15
12
9
6
3
0
1
2
REPLICA
3
Mes 18 de almacenamiento
Analysis of Variance for ACTIVIDAD PROTEOLITICA - Type III Sums of Squares
-------------------------------------------------------------------------------Source
Sum of Squares
Df
Mean Square
F-Ratio
P-Value
-------------------------------------------------------------------------------MAIN EFFECTS
A:INDIVIDUO
4877.92
3
1625.97
79.42
0.0000
B:REPLICA
119.764
2
59.8819
2.92
0.1298
RESIDUAL
122.842
6
20.4737
-------------------------------------------------------------------------------TOTAL (CORRECTED)
5120.53
11
-------------------------------------------------------------------------------All F-ratios are based on the residual mean square error.
ACTIVIDAD PROTEOLITICA
Multiple Range Tests for ACTIVIDAD PROTEOLITICA by REPLICA
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
REPLICA
Count
LS Mean
Homogeneous Groups
-------------------------------------------------------------------------------2
4
15.085
X
1
4
16.02
X
3
4
22.205
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------1 - 2
0.935
9.81704
1 - 3
-6.185
9.81704
2 - 3
-7.12
9.81704
-------------------------------------------------------------------------------* denotes a statistically significant difference.
Scatterplot by Level Code
60
50
40
30
20
10
0
1
2
REPLICA
3
ANEXO 9
ACTIVIDAD PROTEOLITICA - MEDIA DE LAS REPLICAS PARA CADA INDIVIDUO
ALIMETADO CON CADA UNA DE LAS DIETAS EN ESTUDIO
DIETA I O CONTROL
Media de las Réplicas
Mes 0
Mes 3
Mes 6
Mes 9
Mes 12
Mes 18
A
18.85
0.00
31.07
0.00
13.58
0.00
B
6.86
4.63
22.94
18.80
4.62
0.00
C
7.53
9.81
27.81
30.95
0.00
0.00
D
0.00
1.45
9.16
2.97
6.04
0.00
E
0.00
0.00
19.07
17.31
1.72
-
DIETA II (EXCESO DE TOCOFEROLES)
Media de las Réplicas
Mes 0
Mes 3
Mes 6
Mes 9
Mes 12
Mes 18
A
27.35
11.26
11.00
1.33
15.60
5.94
B
2.67
2.67
36.09
13.17
10.58
4.23
C
0.00
8.31
0.00
5.47
10.81
15.15
D
0.00
13.83
18.23
0.00
6.25
27.66
E
71.98
0.00
36.84
7.87
3.45
-
DIETA III (EXTRACTO DE ROMERO)
Media de las Réplicas
A
Mes 0 Mes 3 Mes 6
0.00
11.16 32.23
Mes 9
799.45
Mes 12
0.00
Mes 18
0.00
B
17.77
0.00
0.00
680.65
10.68
0.00
C
0.00
0.00
19.32
284.43
3.16
22.07
D
23.05
26.17
1.67
210.52
0.00
49.01
E
1.12
5.82
10.70
326.35
0.00
-
ANEXO 10
ANALISIS ESTADISTICOS – ANOVA DE DOS VIAS PARA DETERMINAR
DIFERENCIAS ENTRE INDIVIDUOS
A.- DIETA I O CONTROL
Analysis of Variance for ACTIVIDAD PROTEOLITICA - Type III Sums of Squares
-------------------------------------------------------------------------------Source
Sum of Squares
Df
Mean Square
F-Ratio
P-Value
-------------------------------------------------------------------------------MAIN EFFECTS
A:INDIVIDUO
337.686
4
84.4214
1.59
0.2180
B:TIEMPO
1597.5
5
319.499
6.01
0.0017
RESIDUAL
1009.43
19
53.1277
-------------------------------------------------------------------------------TOTAL (CORRECTED)
2911.18
28
-------------------------------------------------------------------------------All F-ratios are based on the residual mean square error.
Multiple Range Tests for ACTIVIDAD PROTEOLITICA by INDIVIDUO
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
INDIVIDUO
Count
LS Mean
Homogeneous Groups
-------------------------------------------------------------------------------D
6
3.27
X
E
5
5.81108
X
B
6
9.64167
X
A
6
10.5833
X
C
6
12.6833
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------A - B
0.941667
12.6592
A - C
-2.1
12.6592
A - D
7.31333
12.6592
A - E
4.77225
13.2771
B - C
-3.04167
12.6592
B - D
6.37167
12.6592
B - E
3.83058
13.2771
C - D
9.41333
12.6592
C - E
6.87225
13.2771
D - E
-2.54108
13.2771
-------------------------------------------------------------------------------* denotes a statistically significant difference.
ACTIVIDAD PROTEOLITICA
Scatterplot by Level Code
40
30
20
10
0
A
B
C
D
E
INDIVIDUO
B.- DIETA II (EXCESO DE TOCOFEROLES)
Analysis of Variance for ACTIVIDAD PROTEOLITICA - Type III Sums of Squares
-------------------------------------------------------------------------------Source
Sum of Squares
Df
Mean Square
F-Ratio
P-Value
-------------------------------------------------------------------------------MAIN EFFECTS
A:INDIVIDUO
930.278
4
232.57
0.95
0.4548
B:TIEMPO
1097.55
5
219.511
0.90
0.5007
RESIDUAL
4630.14
19
243.692
-------------------------------------------------------------------------------TOTAL (CORRECTED)
6618.29
28
-------------------------------------------------------------------------------All F-ratios are based on the residual mean square error.
Multiple Range Tests for ACTIVIDAD PROTEOLITICA by INDIVIDUO
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
INDIVIDUO
Count
LS Mean
Homogeneous Groups
-------------------------------------------------------------------------------C
6
6.62333
X
D
6
10.995
X
B
6
11.5683
X
A
6
12.08
X
E
5
24.6137
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------A - B
0.511667
27.1123
A - C
5.45667
27.1123
A - D
1.085
27.1123
A - E
-12.5337
28.4356
B - C
4.945
27.1123
B - D
0.573333
27.1123
B - E
-13.0453
28.4356
C - D
-4.37167
27.1123
C - E
-17.9903
28.4356
D - E
-13.6187
28.4356
-------------------------------------------------------------------------------* denotes a statistically significant difference.
ACTIVIDAD PROTEOLITICA
Scatterplot by Level Code
80
60
40
20
0
0
3
6
9
12
18
TIEMPO
C.- DIETA III (EXTRACTO DE ROMERO)
Analysis of Variance for ACTIVIDAD PROTEOLITICA - Type III Sums of Squares
-------------------------------------------------------------------------------Source
Sum of Squares
Df
Mean Square
F-Ratio
P-Value
-------------------------------------------------------------------------------MAIN EFFECTS
A:INDIVIDUO
41756.1
4
10439.0
0.84
0.5175
B:TIEMPO
843923.0
5
168785.0
13.56
0.0000
RESIDUAL
236475.0
19
12446.0
-------------------------------------------------------------------------------TOTAL (CORRECTED)
1.11871E6
28
-------------------------------------------------------------------------------All F-ratios are based on the residual mean square error.
Multiple Range Tests for ACTIVIDAD PROTEOLITICA by INDIVIDUO
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
INDIVIDUO
Count
LS Mean
Homogeneous Groups
-------------------------------------------------------------------------------D
6
51.7367
X
E
5
54.0908
X
C
6
54.83
X
B
6
118.183
X
A
6
140.473
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------A - B
22.29
193.759
A - C
85.6433
193.759
A - D
88.7367
193.759
A - E
86.3825
203.216
B - C
63.3533
193.759
B - D
66.4467
193.759
B - E
64.0925
203.216
C - D
3.09333
193.759
C - E
0.739167
203.216
D - E
-2.35417
203.216
-------------------------------------------------------------------------------* denotes a statistically significant difference.
ACTIVIDAD PROTEOLITICA
Scatterplot by Level Code
800
600
400
200
0
A
B
C
D
INDIVIDUO
E
ANEXO 11
ANALISIS ESTADISTICOS – ANOVA DE UNA VIA PARA AP DE INDIVIDUOS
ALIMENTADOS CON DIETA I O CONTROL
ANOVA Table for ACTIVIDAD PROTEOLITICA by TIEMPO
Analysis of Variance
----------------------------------------------------------------------------Source
Sum of Squares
Df Mean Square
F-Ratio
P-Value
----------------------------------------------------------------------------Between groups
1564.07
5
312.814
5.34
0.0021
Within groups
1347.11
23
58.57
----------------------------------------------------------------------------Total (Corr.)
2911.18
28
Multiple Range Tests for ACTIVIDAD PROTEOLITICA by TIEMPO
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
TIEMPO
Count
Mean
Homogeneous Groups
-------------------------------------------------------------------------------18
4
0.0
X
3
5
3.178
X
12
5
5.192
X
0
5
6.648
X
9
5
14.006
XX
6
5
22.01
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------0 - 3
3.47
15.0228
0 - 6
*-15.362
15.0228
0 - 9
-7.358
15.0228
0 - 12
1.456
15.0228
0 - 18
6.648
15.9341
3 - 6
*-18.832
15.0228
3 - 9
-10.828
15.0228
3 - 12
-2.014
15.0228
3 - 18
3.178
15.9341
6 - 9
8.004
15.0228
6 - 12
*16.818
15.0228
6 - 18
*22.01
15.9341
9 - 12
8.814
15.0228
9 - 18
14.006
15.9341
12 - 18
5.192
15.9341
-------------------------------------------------------------------------------* denotes a statistically significant difference.
ACTIVIDAD PROTEOLITICA
Scatterplot by Level Code
40
30
20
10
0
0
3
6
9
12
TIEMPO
Summary Statistics for ACTIVIDAD PROTEOLITICA
TIEMPO
Count
Average
-----------------------------------------------------------0
5
6.648
3
5
3.178
6
5
22.01
9
5
14.006
12
5
5.192
18
4
0.0
-----------------------------------------------------------Total
29
8.79897
TIEMPO
Variance
Standard deviation
-----------------------------------------------------------0
59.5259
7.7153
3
17.3193
4.16164
6
72.5887
8.5199
9
159.74
12.6388
12
27.6041
5.25396
18
0.0
0.0
-----------------------------------------------------------Total
103.971
10.1966
TIEMPO
Minimum
Maximum
-----------------------------------------------------------0
0.0
18.85
3
0.0
9.81
6
9.16
31.07
9
0.0
30.95
12
0.0
13.58
18
0.0
0.0
-----------------------------------------------------------Total
0.0
31.07
TIEMPO
Range
Stnd. skewness
-----------------------------------------------------------0
18.85
1.01854
3
9.81
1.18951
6
21.91
-0.742734
9
30.95
0.173179
12
13.58
1.07207
18
0.0
-----------------------------------------------------------Total
31.07
2.18777
18
TIEMPO
Stnd. kurtosis
-----------------------------------------------------------0
0.5471
3
0.441635
6
0.161117
9
-0.601893
12
0.70849
18
-----------------------------------------------------------Total
-0.254644
Table of Means for ACTIVIDAD PROTEOLITICA by TIEMPO
with 95.0 percent Tukey HSD intervals
-------------------------------------------------------------------------------Stnd. error
TIEMPO
Count
Mean
(pooled s)
Lower limit
Upper limit
-------------------------------------------------------------------------------0
5
6.648
3.42257
-0.863415
14.1594
3
5
3.178
3.42257
-4.33341
10.6894
6
5
22.01
3.42257
14.4986
29.5214
9
5
14.006
3.42257
6.49459
21.5174
12
5
5.192
3.42257
-2.31941
12.7034
18
4
0.0
3.82655
-8.39802
8.39802
-------------------------------------------------------------------------------Total
29
8.79897
Variance Check
Cochran's C test: 0.474318
P-Value = 0.139798
Bartlett's test: 1.33252
P-Value = 0.265503
Hartley's test: 9.22324
Kruskal-Wallis Test for ACTIVIDAD PROTEOLITICA by TIEMPO
TIEMPO
Sample Size
Average Rank
-----------------------------------------------------------0
5
14.0
3
5
11.4
6
5
25.2
9
5
18.3
12
5
13.7
18
4
5.5
-----------------------------------------------------------Test statistic = 14.5773
P-Value = 0.0123301
ANEXO 12
ANALISIS ESTADISTICOS – CORRELACIÓN DE PROPIEDADES FISICAS Y
SENSORIALES CON AP DE MUESTRAS DE INDIVIDUOS ALIMENTADOS CON
DIETA I O CONTROL
Correlations
ACTIVIDAD PROTEOLITICA
-----------------------------------------------------CFU Ent
0.3061
(
6)
0.5552
CFU Prom RAM
0.1877
(
6)
0.7217
Cohesividad Ciz Coc
0.6095
(
6)
0.1990
Cohesividad Ciz Crud
0.6771
(
6)
0.1395
Cohesividad Compresi
0.4963
(
6)
0.3167
Color
-0.1054
(
6)
0.8425
Color Roche desc
0.2712
(
6)
0.6031
Deshidratacion desc
-0.4198
(
6)
0.4073
Dureza N Ciz Coc
0.6862
(
6)
0.1322
Dureza N Ciz Cruda
0.0257
(
6)
0.9614
Dureza N Compresion
0.5253
(
6)
0.2845
Elasticidad desc
0.1670
(
6)
0.7518
GAPING
-0.1835
(
6)
0.7278
Goteo desc
-0.3093
(
6)
0.5508
Olor Putrido desc
-0.5429
(
6)
0.2657
Olor Rancio
-0.4754
(
6)
0.3406
Olor Rancio desc
-0.5492
(
6)
0.2590
ACTIVIDAD PROTEOLITICA
---------------------------------------------------------------Olor Tipico
0.2138
(
6)
0.6841
Olor Tipico desc
0.2968
(
6)
0.5679
pH
-0.6547
(
6)
0.1583
Porcentaje de agua p (dp coc)
0.8182
(
6)
0.0466
Porcentaje de agua p (CRA)
-0.3880
(
6)
0.4472
Porcentaje de agua p (Hum)
-0.0310
(
6)
0.9535
Porcentaje de liq pe (dp cru)
0.4420
(
6)
0.3802
Sabor oxidado
-0.5556
(
6)
0.2523
Sabor Putrido
-0.1376
(
6)
0.7949
Sabor Rancio
-0.5102
(
6)
0.3011
Sabor tipico
0.0547
(
6)
0.9180
TIEMPO
-0.2985
(
6)
0.5656
-----------------------------------------------------------Correlation
(Sample Size)
P-Value
ANEXO 13
ANALISIS ESTADISTICOS – ANOVA DE UNA VIA PARA AP DE INDIVIDUOS
ALIMENTADOS CON DIETA II (EXCESO DE TOCOFEROLES)
ANOVA Table for ACTIVIDAD PROTEOLITICA by TIEMPO
Analysis of Variance
----------------------------------------------------------------------------Source
Sum of Squares
Df Mean Square
F-Ratio
P-Value
----------------------------------------------------------------------------Between groups
1057.87
5
211.574
0.88
0.5130
Within groups
5560.42
23
241.757
----------------------------------------------------------------------------Total (Corr.)
6618.29
28
Multiple Range Tests for ACTIVIDAD PROTEOLITICA by TIEMPO
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
TIEMPO
Count
Mean
Homogeneous Groups
-------------------------------------------------------------------------------9
5
5.568
X
3
5
7.214
X
12
5
9.338
X
18
4
13.245
X
0
5
20.4
X
6
5
20.432
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------0 - 3
13.186
30.5214
0 - 6
-0.032
30.5214
0 - 9
14.832
30.5214
0 - 12
11.062
30.5214
0 - 18
7.155
32.3728
3 - 6
-13.218
30.5214
3 - 9
1.646
30.5214
3 - 12
-2.124
30.5214
3 - 18
-6.031
32.3728
6 - 9
14.864
30.5214
6 - 12
11.094
30.5214
6 - 18
7.187
32.3728
9 - 12
-3.77
30.5214
9 - 18
-7.677
32.3728
12 - 18
-3.907
32.3728
-------------------------------------------------------------------------------* denotes a statistically significant difference.
ACTIVIDAD PROTEOLITICA
Scatterplot by Level Code
80
60
40
20
0
0
3
6
9
12
TIEMPO
Summary Statistics for ACTIVIDAD PROTEOLITICA
TIEMPO
Count
Average
-----------------------------------------------------------0
5
20.4
3
5
7.214
6
5
20.432
9
5
5.568
12
5
9.338
18
4
13.245
-----------------------------------------------------------Total
29
12.6807
TIEMPO
Variance
Standard deviation
-----------------------------------------------------------0
963.868
31.0462
3
33.5081
5.78862
6
256.418
16.0131
9
28.0156
5.29298
12
21.7816
4.66707
18
115.351
10.7402
-----------------------------------------------------------Total
236.368
15.3742
TIEMPO
Minimum
Maximum
-----------------------------------------------------------0
0.0
71.98
3
0.0
13.83
6
0.0
36.84
9
0.0
13.17
12
3.45
15.6
18
4.23
27.66
-----------------------------------------------------------Total
0.0
71.98
TIEMPO
Range
Stnd. skewness
-----------------------------------------------------------0
71.98
1.46188
3
13.83
-0.219849
6
36.84
-0.104041
9
13.17
0.520145
12
12.15
0.0639082
18
23.43
0.825911
-----------------------------------------------------------Total
71.98
5.24385
18
TIEMPO
Stnd. kurtosis
-----------------------------------------------------------0
0.970254
3
-0.948119
6
-0.91977
9
-0.270154
12
-0.239133
18
-0.127424
-----------------------------------------------------------Total
7.89024
Table of Means for ACTIVIDAD PROTEOLITICA by TIEMPO
with 95.0 percent Tukey HSD intervals
-------------------------------------------------------------------------------Stnd. error
TIEMPO
Count
Mean
(pooled s)
Lower limit
Upper limit
-------------------------------------------------------------------------------0
5
20.4
6.95352
5.13932
35.6607
3
5
7.214
6.95352
-8.04668
22.4747
6
5
20.432
6.95352
5.17132
35.6927
9
5
5.568
6.95352
-9.69268
20.8287
12
5
9.338
6.95352
-5.92268
24.5987
18
4
13.245
7.77428
-3.81696
30.307
-------------------------------------------------------------------------------Total
29
12.6807
Variance Check
Cochran's C test: 0.679286
P-Value = 0.0007253
Bartlett's test: 2.672
P-Value = 0.00100631
Hartley's test: 44.2515
Kruskal-Wallis Test for ACTIVIDAD PROTEOLITICA by TIEMPO
TIEMPO
Sample Size
Average Rank
-----------------------------------------------------------0
5
13.5
3
5
13.1
6
5
20.0
9
5
10.8
12
5
15.6
18
4
17.5
-----------------------------------------------------------Test statistic = 3.7338
P-Value = 0.588343
ANEXO 14
ANALISIS ESTADISTICOS – CORRELACIÓN DE PROPIEDADES FISICAS Y
SENSORIALES CON AP DE MUESTRAS DE INDIVIDUOS ALIMENTADOS CON
DIETA II (EXCESO DE TOCOFEROLES)
Correlations
ACTIVIDAD PROTEOLITICA
---------------------------------------------------------------CFU Ent
0.0031
(
6)
0.9954
CFU Prom RAM
-0.4196
(
6)
0.4075
Cohesividad Ciz Coc
-0.0297
(
6)
0.9555
Cohesividad Ciz Crud
0.1521
(
6)
0.7736
Cohesividad Compresi
-0.0592
(
6)
0.9113
Color
0.5534
(
6)
0.2547
Color Roche desc
0.5022
(
6)
0.3101
Deshidratacion desc
-0.5849
(
6)
0.2227
Dureza N Ciz Coc
-0.1889
(
6)
0.7200
Dureza N Ciz Cruda
-0.5041
(
6)
0.3079
Dureza N Compresion
0.1385
(
6)
0.7935
Elasticidad desc
0.0418
(
6)
0.9373
GAPING
-0.1645
(
6)
0.7555
Goteo desc
-0.2682
(
6)
0.6073
Olor Putrido desc
-0.3021
(
6)
0.5606
Olor Rancio
-0.2965
(
6)
0.5683
Olor Rancio desc
0.2129
(
6)
0.6855
Olor Tipico
0.1534
(
6)
0.7716
ACTIVIDAD PROTEOLITICA
---------------------------------------------------------------Olor Tipico desc
0.1151
(
6)
0.8280
pH
0.1004
(
6)
0.8500
Porcentaje de agua p (Dp coc)
-0.1669
(
6)
0.7519
Porcentaje de agua p (CRA)
-0.1062
(
6)
0.8413
Porcentaje de agua p (Hum)
0.3057
(
6)
0.5557
Porcentaje de liq pe (Dp cru)
0.3630
(
6)
0.4794
Sabor oxidado
0.0760
(
6)
0.8862
Sabor Putrido
-0.6364
(
6)
0.1743
Sabor Rancio
-0.3226
(
6)
0.5328
Sabor tipico
-0.0822
(
6)
0.8770
TIEMPO
-0.3068
(
6)
0.5542
-----------------------------------------------------------Correlation
(Sample Size)
P-Value
ANEXO 15
ANALISIS ESTADISTICOS – ANOVA DE UNA VIA PARA AP DE INDIVIDUOS
ALIMENTADOS CON DIETA III (EXTRACTO DE ROMERO)
ANOVA Table for ACTIVIDAD PROTEOLITICA by TIEMPO
Analysis of Variance
----------------------------------------------------------------------------Source
Sum of Squares
Df Mean Square
F-Ratio
P-Value
----------------------------------------------------------------------------Between groups
840479.0
5
168096.0
13.90
0.0000
Within groups
278231.0
23
12097.0
----------------------------------------------------------------------------Total (Corr.)
1.11871E6
28
Multiple Range Tests for ACTIVIDAD PROTEOLITICA by TIEMPO
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
TIEMPO
Count
Mean
Homogeneous Groups
-------------------------------------------------------------------------------12
5
2.768
X
0
5
8.388
X
3
5
8.63
X
6
5
12.784
X
18
4
17.77
X
9
5
460.28
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------0 - 3
-0.242
215.9
0 - 6
-4.396
215.9
0 - 9
*-451.892
215.9
0 - 12
5.62
215.9
0 - 18
-9.382
228.997
3 - 6
-4.154
215.9
3 - 9
*-451.65
215.9
3 - 12
5.862
215.9
3 - 18
-9.14
228.997
6 - 9
*-447.496
215.9
6 - 12
10.016
215.9
6 - 18
-4.986
228.997
9 - 12
*457.512
215.9
9 - 18
*442.51
228.997
12 - 18
-15.002
228.997
-------------------------------------------------------------------------------* denotes a statistically significant difference.
ACTIVIDAD PROTEOLITICA
Scatterplot by Level Code
800
600
400
200
0
0
3
6
9
12
TIEMPO
Summary Statistics for ACTIVIDAD PROTEOLITICA
TIEMPO
Count
Average
-----------------------------------------------------------0
5
8.388
3
5
8.63
6
5
12.784
9
5
460.28
12
5
2.768
18
4
17.77
-----------------------------------------------------------Total
29
87.4252
TIEMPO
Variance
Standard deviation
-----------------------------------------------------------0
124.134
11.1416
3
117.726
10.8501
6
178.04
13.3432
9
68709.9
262.126
12
21.4347
4.62976
18
541.991
23.2807
-----------------------------------------------------------Total
39953.9
199.885
TIEMPO
Minimum
Maximum
-----------------------------------------------------------0
0.0
23.05
3
0.0
26.17
6
0.0
32.23
9
210.52
799.45
12
0.0
10.68
18
0.0
49.01
-----------------------------------------------------------Total
0.0
799.45
TIEMPO
Range
Stnd. skewness
-----------------------------------------------------------0
23.05
0.663759
3
26.17
1.2223
6
32.23
0.666301
9
588.93
0.555391
12
10.68
1.65474
18
49.01
0.834543
-----------------------------------------------------------Total
799.45
6.13317
18
TIEMPO
Stnd. kurtosis
-----------------------------------------------------------0
-1.1836
3
0.70588
6
-0.289128
9
-1.1425
12
1.43354
18
-0.173647
-----------------------------------------------------------Total
8.10826
Table of Means for ACTIVIDAD PROTEOLITICA by TIEMPO
with 95.0 percent Tukey HSD intervals
-------------------------------------------------------------------------------Stnd. error
TIEMPO
Count
Mean
(pooled s)
Lower limit
Upper limit
-------------------------------------------------------------------------------0
5
8.388
49.1874
-99.5621
116.338
3
5
8.63
49.1874
-99.3201
116.58
6
5
12.784
49.1874
-95.1661
120.734
9
5
460.28
49.1874
352.33
568.23
12
5
2.768
49.1874
-105.182
110.718
18
4
17.77
54.9932
-102.922
138.462
-------------------------------------------------------------------------------Total
29
87.4252
Variance Check
Cochran's C test: 0.985891
P-Value = 0.0
Bartlett's test: 34.4865
P-Value = 0.0
Hartley's test: 3205.54
Kruskal-Wallis Test for ACTIVIDAD PROTEOLITICA by TIEMPO
TIEMPO
Sample Size
Average Rank
-----------------------------------------------------------0
5
12.2
3
5
12.8
6
5
15.1
9
5
27.0
12
5
8.9
18
4
13.75
-----------------------------------------------------------Test statistic = 14.0288
P-Value = 0.0154278
ANEXO 16
ANALISIS ESTADISTICOS – CORRELACIÓN DE PROPIEDADES FISICAS Y
SENSORIALES CON AP DE MUESTRAS DE INDIVIDUOS ALIMENTADOS CON
DIETA III (EXTRACTO DE ROMERO)
Correlations
ACTIVIDAD PROTEOLITICA
------------------------------------------------------------CFU Ent
-0.2667
(
6)
0.6094
CFU Prom RAM
0.9783
(
6)
0.0007
Cohesividad Ciz Coc
0.7053
(
6)
0.1175
Cohesividad Ciz Crud
0.3519
(
6)
0.4940
Cohesividad Compresi
0.0868
(
6)
0.8701
Color
-0.3692
(
6)
0.4714
Color Roche desc
-0.2663
(
6)
0.6100
Deshidratacion desc
0.6093
(
6)
0.1991
Dureza N Ciz Coc
0.5196
(
6)
0.2907
Dureza N Ciz Cruda
0.4996
(
6)
0.3129
Dureza N Compresion
-0.1584
(
6)
0.7644
Elasticidad desc
-0.7010
(
6)
0.1208
GAPING
0.0224
(
6)
0.9664
Goteo desc
0.6767
(
6)
0.1399
Olor Putrido desc
-0.3391
(
6)
0.5109
Olor Rancio
-0.0073
(
6)
0.9890
Olor Rancio desc
-0.2488
(
6)
0.6345
ACTIVIDAD PROTEOLITICA
------------------------------------------------------------Olor Tipico
-0.2853
(
6)
0.5836
Olor Tipico desc
0.3014
(
6)
0.5616
pH
-0.7597
(
6)
0.0797
Porcentaje de agua p
-0.0576
(
6)
0.9137
Porcentaje de agua p
-0.0156
(
6)
0.9766
Porcentaje de agua p
-0.2713
(
6)
0.6030
Porcentaje de liq pe
0.2357
(
6)
0.6530
Sabor oxidado
-0.2389
(
6)
0.6484
Sabor Putrido
-0.3041
(
6)
0.5579
Sabor Rancio
0.0388
(
6)
0.9418
Sabor tipico
-0.4643
(
6)
0.3536
TIEMPO
0.0899
(
6)
0.8655
-----------------------------------------------------------Correlation
(Sample Size)
P-Value
ANEXO 17
ANALISIS ESTADISTICOS - ANOVA DE UNA VIA PARA AP DE MUESTRAS DE
INDIVIDUOS ALIMENTADOS CON LAS TRES DIETAS, PARA CADA UNO DE LOS
TIEMPOS ANALIZADOS
Mes 0 de almacenamiento
ANOVA Table for ACTIVIDAD PROTEOLITICA by DIETA
Analysis of Variance
----------------------------------------------------------------------------Source
Sum of Squares
Df Mean Square
F-Ratio
P-Value
----------------------------------------------------------------------------Between groups
1681,89
2
840,946
1,60
0,2149
Within groups
22142,3
42
527,198
----------------------------------------------------------------------------Total (Corr.)
23824,2
44
ACTIVIDAD PROTEOLITICA
Multiple Range Tests for ACTIVIDAD PROTEOLITICA by DIETA
-------------------------------------------------------------------------------Method: 95,0 percent Tukey HSD
DIETA
Count
Mean
Homogeneous Groups
-------------------------------------------------------------------------------1
15
6,64667
X
3
15
8,38933
X
2
15
20,3987
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------1 - 2
-13,752
20,372
1 - 3
-1,74267
20,372
2 - 3
12,0093
20,372
-------------------------------------------------------------------------------* denotes a statistically significant difference.
Scatterplot by Level Code
120
100
80
60
40
20
0
1
2
DIETA
3
Summary Statistics for ACTIVIDAD PROTEOLITICA
DIETA
Count
Average
Variance
Standard
deviation Minimum
----------------------------------------------------------------------------------------1
15
6,64667
123,864
11,1294
0,0
2
15
20,3987
1315,0
36,263
0,0
3
15
8,38933
142,729
11,9469
0,0
----------------------------------------------------------------------------------------Total
45
11,8116
541,46
23,2693
0,0
DIETA
Maximum
Range
Stnd. skewness
Stnd.
kurtosis
----------------------------------------------------------------------------------------1
41,01
41,01
3,68965
5,06317
2
109,22
109,22
3,2092
2,45295
3
32,92
32,92
1,63673
-0,456492
----------------------------------------------------------------------------------------Total
109,22
109,22
8,62584
14,825
Table of Means for ACTIVIDAD PROTEOLITICA by DIETA
with 95,0 percent Tukey HSD intervals
-------------------------------------------------------------------------------Stnd. error
DIETA
Count
Mean
(pooled s)
Lower limit
Upper limit
-------------------------------------------------------------------------------1
15
6,64667
5,92845
-3,53932
16,8327
2
15
20,3987
5,92845
10,2127
30,5847
3
15
8,38933
5,92845
-1,79666
18,5753
-------------------------------------------------------------------------------Total
45
11,8116
Variance Check
Cochran's C test: 0,831441
P-Value = 6,23816E-7
Bartlett's test: 1,8472
P-Value = 0,00000376212
Hartley's test: 10,6165
Kruskal-Wallis Test for ACTIVIDAD PROTEOLITICA by DIETA
DIETA
Sample Size
Average Rank
-----------------------------------------------------------1
15
21,0333
2
15
25,3
3
15
22,6667
-----------------------------------------------------------Test statistic = 0,950065
P-Value = 0,621865
Mes 3 de almacenamiento
ANOVA Table for ACTIVIDAD PROTEOLITICA by DIETA
Analysis of Variance
----------------------------------------------------------------------------Source
Sum of Squares
Df Mean Square
F-Ratio
P-Value
----------------------------------------------------------------------------Between groups
240,104
2
120,052
1,28
0,2898
Within groups
3952,23
42
94,1006
----------------------------------------------------------------------------Total (Corr.)
4192,33
44
ACTIVIDAD PROTEOLITICA
Multiple Range Tests for ACTIVIDAD PROTEOLITICA by DIETA
-------------------------------------------------------------------------------Method: 95,0 percent Tukey HSD
DIETA
Count
Mean
Homogeneous Groups
-------------------------------------------------------------------------------1
15
3,17867
X
2
15
7,21333
X
3
15
8,63133
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------1 - 2
-4,03467
8,60682
1 - 3
-5,45267
8,60682
2 - 3
-1,418
8,60682
-------------------------------------------------------------------------------* denotes a statistically significant difference.
Scatterplot by Level Code
50
40
30
20
10
0
1
2
DIETA
3
Summary Statistics for ACTIVIDAD PROTEOLITICA
DIETA
Count
Average
Variance
Standard
deviation Minimum
----------------------------------------------------------------------------------------1
15
3,17867
25,114
5,01138
0,0
2
15
7,21333
89,561
9,46367
0,0
3
15
8,63133
167,627
12,9471
0,0
----------------------------------------------------------------------------------------Total
45
6,34111
95,2802
9,76116
0,0
DIETA
Maximum
Range
Stnd. skewness
Stnd.
kurtosis
----------------------------------------------------------------------------------------1
14,46
14,46
2,44852
0,804525
2
29,84
29,84
2,32272
1,0801
3
41,57
41,57
2,32099
1,22634
---------------------------------------------------------------------------------------Total
41,57
41,57
5,03634
4,40648
Table of Means for ACTIVIDAD PROTEOLITICA by DIETA
with 95,0 percent Tukey HSD intervals
-------------------------------------------------------------------------------Stnd. error
DIETA
Count
Mean
(pooled s)
Lower limit
Upper limit
-------------------------------------------------------------------------------1
15
3,17867
2,50467
-1,12474
7,48207
2
15
7,21333
2,50467
2,90993
11,5167
3
15
8,63133
2,50467
4,32793
12,9347
-------------------------------------------------------------------------------Total
45
6,34111
Variance Check
Cochran's C test: 0,593786
P-Value = 0,0227948
Bartlett's test: 1,30257
P-Value = 0,00460662
Hartley's test: 6,67465
Kruskal-Wallis Test for ACTIVIDAD PROTEOLITICA by DIETA
DIETA
Sample Size
Average Rank
-----------------------------------------------------------1
15
20,8
2
15
25,0667
3
15
23,1333
-----------------------------------------------------------Test statistic = 0,915942
P-Value = 0,632566
Mes 6 de almacenamiento
ANOVA Table for ACTIVIDAD PROTEOLITICA by DIETA
Analysis of Variance
----------------------------------------------------------------------------Source
Sum of Squares
Df Mean Square
F-Ratio
P-Value
----------------------------------------------------------------------------Between groups
730,096
2
365,048
1,57
0,2201
Within groups
9768,12
42
232,574
----------------------------------------------------------------------------Total (Corr.)
10498,2
44
ACTIVIDAD PROTEOLITICA
Multiple Range Tests for ACTIVIDAD PROTEOLITICA by DIETA
-------------------------------------------------------------------------------Method: 95,0 percent Tukey HSD
DIETA
Count
Mean
Homogeneous Groups
-------------------------------------------------------------------------------3
15
12,7853
X
2
15
20,4313
X
1
15
22,0087
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------1 - 2
1,57733
13,5309
1 - 3
9,22333
13,5309
2 - 3
7,646
13,5309
-------------------------------------------------------------------------------* denotes a statistically significant difference.
Scatterplot by Level Code
60
50
40
30
20
10
0
1
2
DIETA
3
Summary Statistics for ACTIVIDAD PROTEOLITICA
DIETA
Count
Average
Variance
Standard
deviation Minimum
----------------------------------------------------------------------------------------1
15
22,0087
138,848
11,7834
0,0
2
15
20,4313
343,536
18,5347
0,0
3
15
12,7853
215,338
14,6744
0,0
----------------------------------------------------------------------------------------Total
45
18,4084
238,596
15,4465
0,0
DIETA
Maximum
Range
Stnd. skewness
Stnd.
kurtosis
----------------------------------------------------------------------------------------1
45,3
45,3
-0,373748
0,163642
2
50,93
50,93
0,167067
-1,16718
3
39,97
39,97
0,915959
-1,0568
----------------------------------------------------------------------------------------Total
50,93
50,93
0,409408
-1,55625
Table of Means for ACTIVIDAD PROTEOLITICA by DIETA
with 95,0 percent Tukey HSD intervals
-------------------------------------------------------------------------------Stnd. error
DIETA
Count
Mean
(pooled s)
Lower limit
Upper limit
-------------------------------------------------------------------------------1
15
22,0087
3,93763
15,2432
28,7741
2
15
20,4313
3,93763
13,6659
27,1968
3
15
12,7853
3,93763
6,01988
19,5508
-------------------------------------------------------------------------------Total
45
18,4084
Variance Check
Cochran's C test: 0,492368
P-Value = 0,197969
Bartlett's test: 1,06992
P-Value = 0,252703
Hartley's test: 2,47418
Kruskal-Wallis Test for ACTIVIDAD PROTEOLITICA by DIETA
DIETA
Sample Size
Average Rank
-----------------------------------------------------------1
15
25,9667
2
15
24,9333
3
15
18,1
-----------------------------------------------------------Test statistic = 3,23919
P-Value = 0,197979
Mes 9 de almacenamiento
ANOVA Table for ACTIVIDAD PROTEOLITICA by DIETA
Analysis of Variance
----------------------------------------------------------------------------Source
Sum of Squares
Df Mean Square
F-Ratio
P-Value
----------------------------------------------------------------------------Between groups
2,41903E6
2
1,20951E6
42,95
0,0000
Within groups
1,18282E6
42
28162,5
----------------------------------------------------------------------------Total (Corr.)
3,60185E6
44
ACTIVIDAD PROTEOLITICA
Multiple Range Tests for ACTIVIDAD PROTEOLITICA by DIETA
-------------------------------------------------------------------------------Method: 95,0 percent Tukey HSD
DIETA
Count
Mean
Homogeneous Groups
-------------------------------------------------------------------------------2
15
5,56733
X
1
15
14,0053
X
3
15
501,568
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------1 - 2
8,438
148,896
1 - 3
*-487,563
148,896
2 - 3
*-496,001
148,896
-------------------------------------------------------------------------------* denotes a statistically significant difference.
Scatterplot by Level Code
1200
1000
800
600
400
200
0
1
2
DIETA
3
Summary Statistics for ACTIVIDAD PROTEOLITICA
DIETA
Count
Average
Variance
Standard
deviation Minimum
----------------------------------------------------------------------------------------1
15
14,0053
172,576
13,1368
0,0
2
15
5,56733
58,4418
7,64472
0,0
3
15
501,568
84256,4
290,27
174,08
----------------------------------------------------------------------------------------Total
45
173,714
81860,2
286,112
0,0
DIETA
Maximum
Range
Stnd. skewness
Stnd.
kurtosis
----------------------------------------------------------------------------------------1
34,45
34,45
0,456684
-1,15056
2
30,52
30,52
4,30559
7,01468
3
1014,86
840,78
0,796219
-1,04856
----------------------------------------------------------------------------------------Total
1014,86
1014,86
4,73973
2,56997
Table of Means for ACTIVIDAD PROTEOLITICA by DIETA
with 95,0 percent Tukey HSD intervals
-------------------------------------------------------------------------------Stnd. error
DIETA
Count
Mean
(pooled s)
Lower limit
Upper limit
-------------------------------------------------------------------------------1
15
14,0053
43,3301
-60,4425
88,4531
2
15
5,56733
43,3301
-68,8805
80,0151
3
15
501,568
43,3301
427,12
576,016
-------------------------------------------------------------------------------Total
45
173,714
Variance Check
Cochran's C test: 0,997266
P-Value = 0,0
Bartlett's test: 29,7328
P-Value = 0,0
Hartley's test: 1441,72
Kruskal-Wallis Test for ACTIVIDAD PROTEOLITICA by DIETA
DIETA
Sample Size
Average Rank
-----------------------------------------------------------1
15
17,7
2
15
13,3
3
15
38,0
-----------------------------------------------------------Test statistic = 30,5213
P-Value = 2,35711E-7
Mes 12 de almacenamiento
ANOVA Table for ACTIVIDAD PROTEOLITICA by DIETA
Analysis of Variance
----------------------------------------------------------------------------Source
Sum of Squares
Df Mean Square
F-Ratio
P-Value
----------------------------------------------------------------------------Between groups
331,424
2
165,712
3,65
0,0346
Within groups
1907,7
42
45,4213
----------------------------------------------------------------------------Total (Corr.)
2239,12
44
ACTIVIDAD PROTEOLITICA
Multiple Range Tests for ACTIVIDAD PROTEOLITICA by DIETA
-------------------------------------------------------------------------------Method: 95,0 percent Tukey HSD
DIETA
Count
Mean
Homogeneous Groups
-------------------------------------------------------------------------------3
15
2,76667
X
1
15
5,19133
XX
2
15
9,33933
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------1 - 2
-4,148
5,97966
1 - 3
2,42467
5,97966
2 - 3
*6,57267
5,97966
-------------------------------------------------------------------------------* denotes a statistically significant difference.
Scatterplot by Level Code
30
25
20
15
10
5
0
1
2
DIETA
3
Summary Statistics for ACTIVIDAD PROTEOLITICA
DIETA
Count
Average
Variance
Standard
deviation Minimum
----------------------------------------------------------------------------------------1
15
5,19133
41,1651
6,41601
0,0
2
15
9,33933
68,6639
8,28637
0,0
3
15
2,76667
26,435
5,14149
0,0
----------------------------------------------------------------------------------------Total
45
5,76578
50,8891
7,13366
0,0
DIETA
Maximum
Range
Stnd. skewness
Stnd.
kurtosis
----------------------------------------------------------------------------------------1
17,88
17,88
1,63732
-0,174124
2
29,04
29,04
1,42036
0,448942
3
17,82
17,82
3,37448
3,67727
----------------------------------------------------------------------------------------Total
29,04
29,04
3,46279
1,54092
Table of Means for ACTIVIDAD PROTEOLITICA by DIETA
with 95,0 percent Tukey HSD intervals
-------------------------------------------------------------------------------Stnd. error
DIETA
Count
Mean
(pooled s)
Lower limit
Upper limit
-------------------------------------------------------------------------------1
15
5,19133
1,74014
2,20151
8,18116
2
15
9,33933
1,74014
6,34951
12,3292
3
15
2,76667
1,74014
-0,223162
5,75649
-------------------------------------------------------------------------------Total
45
5,76578
Variance Check
Cochran's C test: 0,503903
P-Value = 0,161185
Bartlett's test: 1,0784
P-Value = 0,21517
Hartley's test: 2,59746
Kruskal-Wallis Test for ACTIVIDAD PROTEOLITICA by DIETA
DIETA
Sample Size
Average Rank
-----------------------------------------------------------1
15
21,8667
2
15
30,4333
3
15
16,7
-----------------------------------------------------------Test statistic = 8,93955
P-Value = 0,0114499
Mes 18 de almacenamiento
ANOVA Table for ACTIVIDAD PROTEOLITICA by DIETA
Analysis of Variance
----------------------------------------------------------------------------Source
Sum of Squares
Df Mean Square
F-Ratio
P-Value
----------------------------------------------------------------------------Between groups
2046,71
2
1023,36
5,26
0,0104
Within groups
6421,15
33
194,58
----------------------------------------------------------------------------Total (Corr.)
8467,86
35
ACTIVIDAD PROTEOLITICA
Multiple Range Tests for ACTIVIDAD PROTEOLITICA by DIETA
-------------------------------------------------------------------------------Method: 95,0 percent Tukey HSD
DIETA
Count
Mean
Homogeneous Groups
-------------------------------------------------------------------------------1
12
0,0
X
2
12
13,245
XX
3
12
17,77
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------1 - 2
-13,245
13,9766
1 - 3
*-17,77
13,9766
2 - 3
-4,525
13,9766
-------------------------------------------------------------------------------* denotes a statistically significant difference.
Scatterplot by Level Code
60
50
40
30
20
10
0
1
2
DIETA
3
Summary Statistics for ACTIVIDAD PROTEOLITICA
DIETA
Count
Average
Variance
Standard
deviation Minimum
----------------------------------------------------------------------------------------1
12
0,0
0,0
0,0
0,0
2
12
13,245
118,238
10,8737
0,0
3
12
17,77
465,502
21,5755
0,0
----------------------------------------------------------------------------------------Total
36
10,3383
241,939
15,5544
0,0
DIETA
Maximum
Range
Stnd. skewness
Stnd.
kurtosis
----------------------------------------------------------------------------------------1
0,0
0,0
2
33,89
33,89
0,614313
-0,27783
3
56,88
56,88
1,07376
-0,743621
----------------------------------------------------------------------------------------Total
56,88
56,88
3,79026
2,01958
Table of Means for ACTIVIDAD PROTEOLITICA by DIETA
with 95,0 percent Tukey HSD intervals
-------------------------------------------------------------------------------Stnd. error
DIETA
Count
Mean
(pooled s)
Lower limit
Upper limit
-------------------------------------------------------------------------------1
12
0,0
4,02679
-6,98828
6,98828
2
12
13,245
4,02679
6,25672
20,2333
3
12
17,77
4,02679
10,7817
24,7583
-------------------------------------------------------------------------------Total
36
10,3383
Variance Check
Cochran's C test: 0,797447
P-Value = 0,0319819
Bartlett's test: 1,24409
P-Value = 0,0320453
Hartley's test: 3,93699
Kruskal-Wallis Test for ACTIVIDAD PROTEOLITICA by DIETA
DIETA
Sample Size
Average Rank
-----------------------------------------------------------1
12
11,0
2
12
23,0
3
12
21,5
-----------------------------------------------------------Test statistic = 11,5281
P-Value = 0,00313839
ANEXO 18
ANALISIS ESTADISTICOS – ANOVA DE DOS VIAS PARA ANALIZAR
COMPORTAMIENTO DE GSH - Px FRENTE A LA CANTIDAD
DE PEROXIDO DE HIDROGENO
Analysis of Variance for ABSORBANCIA - Type III Sums of Squares
-------------------------------------------------------------------------------Source
Sum of Squares
Df
Mean Square
F-Ratio
P-Value
-------------------------------------------------------------------------------MAIN EFFECTS
A:PEROXIDO DE HIDR
0.0276398
2
0.0138199
5985.00
0.0000
B:TIEMPO
0.0220229
10
0.00220229
953.75
0.0000
RESIDUAL
0.0000461818
20 0.00000230909
-------------------------------------------------------------------------------TOTAL (CORRECTED)
0.0497089
32
-------------------------------------------------------------------------------All F-ratios are based on the residual mean square error.
Multiple Range Tests for ABSORBANCIA by PEROXIDO DE HIDROGENO
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
Level
Count
LS Mean
Homogeneous Groups
-------------------------------------------------------------------------------50
11
1.04564
X
30
11
1.04782
X
100
11
1.10809
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------30 - 50
*0.00218182
0.00163996
30 - 100
*-0.0602727
0.00163996
50 - 100
*-0.0624545
0.00163996
-------------------------------------------------------------------------------* denotes a statistically significant difference.
Scatterplot by Level Code
ABSORBANCIA
1,15
1,12
1,09
1,06
1,03
1
30
50
100
PEROXIDO DE HIDROGENO
ANEXO 19
ANALISIS ESTADISTICOS – ANOVA DE DOS VIAS PARA ANALIZAR
COMPORTAMIENTO DE DE GSH - Px A LA CANTIDAD
DE GLUTATION REDUCIDO
Analysis of Variance for ABSORBANCIA - Type III Sums of Squares
-------------------------------------------------------------------------------Source
Sum of Squares
Df
Mean Square
F-Ratio
P-Value
-------------------------------------------------------------------------------MAIN EFFECTS
A:GLUTATION REDUCT
0.366548
3
0.122183
1764.10
0.0000
B:TIEMPO
0.0251747
10
0.00251747
36.35
0.0000
RESIDUAL
0.00207782
30
0.0000692606
-------------------------------------------------------------------------------TOTAL (CORRECTED)
0.393801
43
-------------------------------------------------------------------------------All F-ratios are based on the residual mean square error.
Multiple Range Tests for ABSORBANCIA by GLUTATION REDUCTASA
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
Level
Count
LS Mean
Homogeneous Groups
-------------------------------------------------------------------------------6
11
0.484909
X
2
11
0.516909
X
10
11
0.681727
X
4
11
0.682364
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------2 - 4
*-0.165455
0.00965087
2 - 6
*0.032
0.00965087
2 - 10
*-0.164818
0.00965087
4 - 6
*0.197455
0.00965087
4 - 10
0.000636364
0.00965087
6 - 10
*-0.196818
0.00965087
-------------------------------------------------------------------------------* denotes a statistically significant difference.
Scatterplot by Level Code
Absorbancia
0,83
0,73
0,63
0,53
0,43
2
4
6
GRd
10
ANEXO 20
ANALISIS ESTADISTICOS – ANOVA DE DOS VIAS PARA ANALIZAR
COMPORTAMIENTO DE GSH - Px AL VARIAR
SU CANTIDAD EN LA MEZCLA REACTANTE
Analysis of Variance for ABSORBANCIA - Type III Sums of Squares
-------------------------------------------------------------------------------Source
Sum of Squares
Df
Mean Square
F-Ratio
P-Value
-------------------------------------------------------------------------------MAIN EFFECTS
A:MUESTRA
0.0588705
2
0.0294353
6344.64
0.0000
B:TIEMPO
0.0115914
10
0.00115914
249.85
0.0000
RESIDUAL
0.0000927879
20 0.00000463939
-------------------------------------------------------------------------------TOTAL (CORRECTED)
0.0705547
32
-------------------------------------------------------------------------------All F-ratios are based on the residual mean square error.
Multiple Range Tests for ABSORBANCIA by MUESTRA
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
MUESTRA
Count
LS Mean
Homogeneous Groups
-------------------------------------------------------------------------------50
11
0.781455
X
100
11
0.832364
X
150
11
0.884909
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------50 - 100
*-0.0509091
0.00232458
50 - 150
*-0.103455
0.00232458
100 - 150
*-0.0525455
0.00232458
-------------------------------------------------------------------------------* denotes a statistically significant difference.
Scatterplot by Level Code
Absorbancia
0,93
0,9
0,87
0,84
0,81
0,78
0,75
50
100
Muestra
150
ANEXO 21
ACTIVIDAD DE GLUTATION PEROXIDASA PARA PARA MUSCULO DE SALMON
COHO PROVENIENTE DE INDIVIDUOS ALIMENTADOS CON LAS DIETAS
ANALIZADAS
DIETA I O CONTROL
Individuo
A
B
C
D
E
Mes 0
9066.6
5922.7
5296.6
6179.3
16085.3
Mes 3
Mes 6
Mes 9
Mes 12
Mes 18
9693.0
15074.3
13730.2
11397.4
7316.2
11428.4
13382.8
10889.4
11618.3
15353.4
-
-
-
-
17278.4
-
-
-
-
16428.4
-
-
-
-
-
Mes 12
11298.8
12251.7
-
Mes 18
19871.9
12871.3
13406.4
15308.0
-
Mes 12
13143.3
15034.1
-
Mes 18
13149.8
12117.0
11535.5
11206.0
-
DIETA II (EXCESO DE TOCOFEROLES)
Individuo
A
B
C
D
E
Mes 0
12271.1
14466.7
18159.5
16123.5
20748.4
Mes 3
10931.0
10693.4
-
Mes 6
12378.1
12928.2
-
Mes 9
13169.1
13032.4
-
DIETA III (EXTRACTO DE ROMERO)
Individuo
A
B
C
D
E
Mes 0
13077.0
14510.9
11453.5
13340.5
12477.1
Mes 3
12186.5
5654.5
-
Mes 6
6831.9
8885.7
-
Mes 9
11102.9
10800.5
-
ANEXO 22
ANALISIS ESTADISTICOS – ANOVA DE DOS VIAS PARA DETERMINAR
DIFERENCIAS ESTADISTICAS ENTRE INDIVIDUOS
A.- DIETA I O CONTROL
Analysis of Variance for GLUTATION PEROXIDASA - Type III Sums of Squares
-------------------------------------------------------------------------------Source
Sum of Squares
Df
Mean Square
F-Ratio
P-Value
-------------------------------------------------------------------------------MAIN EFFECTS
A:INDIVIDUO
7.92533E7
4
1.98133E7
1.95
0.2064
B:TIEMPO
1.4638E8
5
2.92759E7
2.89
0.0998
RESIDUAL
7.10043E7
7
1.01435E7
-------------------------------------------------------------------------------TOTAL (CORRECTED)
2.39815E8
16
-------------------------------------------------------------------------------All F-ratios are based on the residual mean square error.
Multiple Range Tests for GLUTATION PEROXIDASA by INDIVIDUO
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
INDIVIDUO
Count
LS Mean
Homogeneous Groups
-------------------------------------------------------------------------------A
6
11046.3
X
B
6
11432.5
X
C
2
13112.2
X
D
2
13128.5
X
E
1
21648.9
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------A - B
-386.217
6560.89
A - C
-2065.88
9278.51
A - D
-2082.23
9278.51
A - E
-10602.6
12274.3
B - C
-1679.67
9278.51
B - D
-1696.02
9278.51
B - E
-10216.4
12274.3
C - D
-16.35
11363.8
C - E
-8536.7
13917.8
D - E
-8520.35
13917.8
-------------------------------------------------------------------------------* denotes a statistically significant difference.
GLUTATION PEROXIDASA
Scatterplot by Level Code
(X 1000)
20
17
14
11
8
5
A
B
C
D
E
INDIVIDUO
B.- DIETA II (EXCESO DE TOCOFEROLES)
Analysis of Variance for GLUTATION PEROXIDASA - Type III Sums of Squares
-------------------------------------------------------------------------------Source
Sum of Squares
Df
Mean Square
F-Ratio
P-Value
-------------------------------------------------------------------------------MAIN EFFECTS
A:INDIVIDUO
2.68166E7
4
6.70415E6
1.00
0.4683
B:TIEMPO
2.68468E7
5
5.36937E6
0.80
0.5837
RESIDUAL
4.7052E7
7
6.72172E6
-------------------------------------------------------------------------------TOTAL (CORRECTED)
1.44272E8
16
-------------------------------------------------------------------------------All F-ratios are based on the residual mean square error.
Multiple Range Tests for GLUTATION PEROXIDASA by INDIVIDUO
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
INDIVIDUO
Count
LS Mean
Homogeneous Groups
-------------------------------------------------------------------------------B
6
12707.3
X
A
6
13320.0
X
D
2
13859.1
X
C
2
13926.3
X
E
1
18946.4
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------A - B
612.717
5340.84
A - C
-606.342
7553.09
A - D
-539.142
7553.09
A - E
-5626.39
9991.8
B - C
-1219.06
7553.09
B - D
-1151.86
7553.09
B - E
-6239.11
9991.8
C - D
67.2
9250.61
C - E
-5020.05
11329.6
D - E
-5087.25
11329.6
-------------------------------------------------------------------------------* denotes a statistically significant difference.
GLUTATION PEROXIDASA
Scatterplot by Level Code
(X 10000)
2.2
2
1.8
1.6
1.4
1.2
1
A
B
C
D
E
INDIVIDUO
C.- DIETA III (EXTRACTO DE ROMERO)
Analysis of Variance for GLUTATION PEROXIDASA - Type III Sums of Squares
-------------------------------------------------------------------------------Source
Sum of Squares
Df
Mean Square
F-Ratio
P-Value
-------------------------------------------------------------------------------MAIN EFFECTS
A:INDIVIDUO
4.96523E6
4
1.24131E6
0.32
0.8592
B:TIEMPO
6.77771E7
5
1.35554E7
3.44
0.0689
RESIDUAL
2.75583E7
7
3.9369E6
-------------------------------------------------------------------------------TOTAL (CORRECTED)
9.81315E7
16
-------------------------------------------------------------------------------All F-ratios are based on the residual mean square error.
Multiple Range Tests for GLUTATION PEROXIDASA by INDIVIDUO
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
INDIVIDUO
Count
LS Mean
Homogeneous Groups
-------------------------------------------------------------------------------C
2
9655.33
X
E
1
10091.2
X
D
2
10434.1
X
B
6
11167.1
X
A
6
11581.9
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------A - B
414.783
4087.4
A - C
1926.57
5780.46
A - D
1147.82
5780.46
A - E
1490.67
7646.83
B - C
1511.78
5780.46
B - D
733.033
5780.46
B - E
1075.88
7646.83
C - D
-778.75
7079.58
C - E
-435.9
8670.68
D - E
342.85
8670.68
-------------------------------------------------------------------------------* denotes a statistically significant difference.
GLUTATION PEROXIDASA
Scatterplot by Level Code
15600
13600
11600
9600
7600
5600
A
B
C
D
INDIVIDUO
E
ANEXO 23
ANALISIS ESTADISTICOS – ANOVA DE UNA VIA PARA GSH – Px DE INDIVIDUOS
ALIMENTADOS CON LA DIETA I O CONTROL
ANOVA Table for GLUTATION PEROXIDASA by TIEMPO
Analysis of Variance
----------------------------------------------------------------------------Source
Sum of Squares
Df Mean Square
F-Ratio
P-Value
----------------------------------------------------------------------------Between groups
8.9557E7
5
1.79114E7
1.31
0.3278
Within groups
1.50258E8
11
1.36598E7
----------------------------------------------------------------------------Total (Corr.)
2.39815E8
16
Multiple Range Tests for GLUTATION PEROXIDASA by TIEMPO
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
TIEMPO
Count
Mean
Homogeneous Groups
-------------------------------------------------------------------------------0
5
8510.1
X
3
2
10560.7
X
12
2
11507.8
X
9
2
12309.8
X
18
4
14094.1
X
6
2
14228.5
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------0 - 3
-2050.6
10537.8
0 - 6
-5718.45
10537.8
0 - 9
-3799.7
10537.8
0 - 12
-2997.75
10537.8
0 - 18
-5584.0
8449.0
3 - 6
-3667.85
12595.0
3 - 9
-1749.1
12595.0
3 - 12
-947.15
12595.0
3 - 18
-3533.4
10907.6
6 - 9
1918.75
12595.0
6 - 12
2720.7
12595.0
6 - 18
134.45
10907.6
9 - 12
801.95
12595.0
9 - 18
-1784.3
10907.6
12 - 18
-2586.25
10907.6
-------------------------------------------------------------------------------* denotes a statistically significant difference.
GLUTATION PEROXIDASA
Scatterplot by Level Code
(X 1000)
20
17
14
11
8
5
0
3
6
9
12
TIEMPO
Summary Statistics for GLUTATION PEROXIDASA
TIEMPO
Count
Average
-----------------------------------------------------------0
5
8510.1
3
2
10560.7
6
2
14228.5
9
2
12309.8
12
2
11507.8
18
4
14094.1
-----------------------------------------------------------Total
17
11537.7
TIEMPO
Variance
Standard deviation
-----------------------------------------------------------0
2.00368E7
4476.25
3
1.50581E6
1227.11
6
1.43059E6
1196.07
9
4.03507E6
2008.75
12
24398.4
156.2
18
2.10382E7
4586.74
-----------------------------------------------------------Total
1.49884E7
3871.49
TIEMPO
Minimum
Maximum
-----------------------------------------------------------0
5296.6
16085.3
3
9693.0
11428.4
6
13382.8
15074.3
9
10889.4
13730.2
12
11397.4
11618.3
18
7316.2
17278.4
-----------------------------------------------------------Total
5296.6
17278.4
TIEMPO
Range
Stnd. skewness
-----------------------------------------------------------0
10788.7
1.57632
3
1735.4
6
1691.5
9
2840.8
12
220.9
18
9962.2
-1.4913
-----------------------------------------------------------Total
11981.8
-0.316474
18
TIEMPO
Stnd. kurtosis
-----------------------------------------------------------0
1.28544
3
6
9
12
18
1.39264
-----------------------------------------------------------Total
-0.965578
Table of Means for GLUTATION PEROXIDASA by TIEMPO
with 95.0 percent Tukey HSD intervals
-------------------------------------------------------------------------------Stnd. error
TIEMPO
Count
Mean
(pooled s)
Lower limit
Upper limit
-------------------------------------------------------------------------------0
5
8510.1
1652.86
4527.2
12493.0
3
2
10560.7
2613.41
4263.19
16858.2
6
2
14228.5
2613.41
7931.04
20526.1
9
2
12309.8
2613.41
6012.29
18607.3
12
2
11507.8
2613.41
5210.34
17805.4
18
4
14094.1
1847.96
9641.09
18547.1
-------------------------------------------------------------------------------Total
17
11537.7
Variance Check
Cochran's C test: 0.437649
P-Value = 0.37421
Bartlett's test: 2.30387
P-Value = 0.215874
Hartley's test: 862.276
Kruskal-Wallis Test for GLUTATION PEROXIDASA by TIEMPO
TIEMPO
Sample Size
Average Rank
-----------------------------------------------------------0
5
5.2
3
2
7.5
6
2
12.0
9
2
9.5
12
2
9.0
18
4
12.75
-----------------------------------------------------------Test statistic = 5.93922
P-Value = 0.312178
ANEXO 24
ANALISIS ESTADISTICOS – CORRELACIÓN DE PROPIEDADES FISICAS Y
SENSORIALES CON GSH - Px DE MUESTRAS DE INDIVIDUOS ALIMENTADOS
CON DIETA I O CONTROL
Correlations
GLUTATION PEROXIDASA
-----------------------------------------------------------CFU Ent
0.4485
(
6)
0.3724
CFU Prom RAM
-0.3769
(
6)
0.4615
Cohesividad Ciz Coc
0.4792
(
6)
0.3363
Cohesividad Ciz Crud
0.1065
(
6)
0.8409
Cohesividad Compresi
0.0937
(
6)
0.8599
Color
0.3838
(
6)
0.4526
Color Roche desc
-0.0888
(
6)
0.8672
Deshidratacion desc
0.0989
(
6)
0.8521
Dureza N Ciz Coc
0.6498
(
6)
0.1625
Dureza N Ciz Cruda
0.3976
(
6)
0.4350
Dureza N Compresion
0.4775
(
6)
0.3382
Elasticidad desc
-0.4063
(
6)
0.4241
GAPING
-0.0126
(
6)
0.9811
Goteo desc
0.3086
(
6)
0.5518
Olor Putrido desc
-0.0168
(
6)
0.9748
Olor Rancio
0.1277
(
6)
0.8096
Olor Rancio desc
0.0458
(
6)
0.9314
GLUTATION PEROXIDASA
-----------------------------------------------------------Olor Tipico
-0.4146
(
6)
0.4137
Olor Tipico desc
-0.3405
(
6)
0.5090
pH
-0.8201
(
6)
0.0456
Porcentaje de agua p
0.2834
(
6)
0.5862
Porcentaje de agua p
-0.1202
(
6)
0.8205
Porcentaje de agua p
0.3971
(
6)
0.4357
Porcentaje de liq pe
0.0229
(
6)
0.9657
Sabor oxidado
0.0434
(
6)
0.9350
Sabor Putrido
0.3164
(
6)
0.5412
Sabor Rancio
0.0344
(
6)
0.9484
Sabor tipico
-0.5646
(
6)
0.2431
TIEMPO
0.3645
(
6)
0.4775
-----------------------------------------------------------Correlation
(Sample Size)
P-Value
ANEXO 25
ANALISIS ESTADISTICOS – CORRELACIÓN DE INDICADORES DE
OXIDACION LIPIDICA CON GSH - Px DE MUESTRAS DE INDIVIDUOS
ALIMENTADOS CON DIETA I O CONTROL
Correlations
GLUTATION PEROXIDASA
-----------------------------------------------------------DMA
0.5467
(
5)
0.3404
HCHO
0.8090
(
5)
0.0973
INDICE DE PEROXIDOS
0.8746
(
5)
0.0523
INDICE DE POLIENOS
-0.5080
(
5)
0.3822
NBVT
0.3476
(
5)
0.5665
P ANISIDINA
0.2732
(
5)
0.6565
TIEMPO
0.5790
(
5)
0.3064
-----------------------------------------------------------Correlation
(Sample Size)
P-Value
ANEXO 26
ANALISIS ESTADISTICOS – ANALISIS ESTADISTICOS – ANOVA DE UNA VIA
PARA GSH – Px DE INDIVIDUOS ALIMENTADOS CON
DIETA II (EXCESO DE TOCOFEROLES)
ANOVA Table for GLUTATION PEROXIDASA by TIEMPO
Analysis of Variance
----------------------------------------------------------------------------Source
Sum of Squares
Df Mean Square
F-Ratio
P-Value
----------------------------------------------------------------------------Between groups
7.04032E7
5
1.40806E7
2.10
0.1422
Within groups
7.38686E7
11
6.71533E6
----------------------------------------------------------------------------Total (Corr.)
1.44272E8
16
Multiple Range Tests for GLUTATION PEROXIDASA by TIEMPO
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
TIEMPO
Count
Mean
Homogeneous Groups
-------------------------------------------------------------------------------3
2
10812.2
X
12
2
11775.3
X
6
2
12653.2
X
9
2
13100.8
X
18
4
15364.4
X
0
5
16353.8
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------0 - 3
5541.64
7388.56
0 - 6
3700.69
7388.56
0 - 9
3253.09
7388.56
0 - 12
4578.59
7388.56
0 - 18
989.44
5924.03
3 - 6
-1840.95
8831.02
3 - 9
-2288.55
8831.02
3 - 12
-963.05
8831.02
3 - 18
-4552.2
7647.89
6 - 9
-447.6
8831.02
6 - 12
877.9
8831.02
6 - 18
-2711.25
7647.89
9 - 12
1325.5
8831.02
9 - 18
-2263.65
7647.89
12 - 18
-3589.15
7647.89
-------------------------------------------------------------------------------* denotes a statistically significant difference.
GLUTATION PEROXIDASA
Scatterplot by Level Code
(X 10000)
2.2
2
1.8
1.6
1.4
1.2
1
0
3
6
9
12
TIEMPO
Summary Statistics for GLUTATION PEROXIDASA
TIEMPO
Count
Average
-----------------------------------------------------------0
5
16353.8
3
2
10812.2
6
2
12653.2
9
2
13100.8
12
2
11775.3
18
4
15364.4
-----------------------------------------------------------Total
17
14112.3
TIEMPO
Variance
Standard deviation
-----------------------------------------------------------0
1.07139E7
3273.21
3
28226.9
168.009
6
151305.0
388.979
9
9343.45
96.6615
12
454009.0
673.802
18
1.01233E7
3181.72
-----------------------------------------------------------Total
9.01699E6
3002.83
TIEMPO
Minimum
Maximum
-----------------------------------------------------------0
12271.1
20748.4
3
10693.4
10931.0
6
12378.1
12928.2
9
13032.4
13169.1
12
11298.8
12251.7
18
12871.3
19871.9
-----------------------------------------------------------Total
10693.4
20748.4
TIEMPO
Range
Stnd. skewness
-----------------------------------------------------------0
8477.3
0.173197
3
237.6
6
550.1
9
136.7
12
952.9
18
7000.6
1.15895
-----------------------------------------------------------Total
10055.0
1.94247
18
TIEMPO
Stnd. kurtosis
-----------------------------------------------------------0
-0.300813
3
6
9
12
18
0.678287
-----------------------------------------------------------Total
0.379381
Table of Means for GLUTATION PEROXIDASA by TIEMPO
with 95.0 percent Tukey HSD intervals
-------------------------------------------------------------------------------Stnd. error
TIEMPO
Count
Mean
(pooled s)
Lower limit
Upper limit
-------------------------------------------------------------------------------0
5
16353.8
1158.91
13561.2
19146.5
3
2
10812.2
1832.39
6396.69
15227.7
6
2
12653.2
1832.39
8237.64
17068.7
9
2
13100.8
1832.39
8685.24
17516.3
12
2
11775.3
1832.39
7359.74
16190.8
18
4
15364.4
1295.7
12242.2
18486.6
-------------------------------------------------------------------------------Total
17
14112.3
Variance Check
Cochran's C test: 0.498782
P-Value = 0.219113
Bartlett's test: 4.06859
P-Value = 0.0364887
Hartley's test: 1146.68
Kruskal-Wallis Test for GLUTATION PEROXIDASA by TIEMPO
TIEMPO
Sample Size
Average Rank
-----------------------------------------------------------0
5
12.6
3
2
1.5
6
2
7.0
9
2
9.5
12
2
3.5
18
4
11.75
-----------------------------------------------------------Test statistic = 10.8451
P-Value = 0.0545392
ANEXO 27
ANALISIS ESTADISTICOS – CORRELACIÓN DE PROPIEDADES FISICAS Y
SENSORIALES CON GSH - Px DE MUESTRAS DE INDIVIDUOS ALIMENTADOS
CON LA DIETA II (EXCESO DE TOCOFEROLES)
Correlations
GLUTATION PEROXIDASA
-----------------------------------------------------------CFU Ent
0.0448
(
6)
0.9328
CFU Prom RAM
0.0063
(
6)
0.9905
Cohesividad Ciz Coc
0.1274
(
6)
0.8099
Cohesividad Ciz Crud
-0.3335
(
6)
0.5183
Cohesividad Compresi
-0.6161
(
6)
0.1928
Color
-0.1600
(
6)
0.7620
Color Roche desc
-0.1820
(
6)
0.7301
Deshidratacion desc
0.0103
(
6)
0.9846
Dureza N Ciz Coc
-0.6926
(
6)
0.1272
Dureza N Ciz Cruda
0.0136
(
6)
0.9796
Dureza N Compresion
0.1916
(
6)
0.7161
Elasticidad desc
-0.1017
(
6)
0.8480
GAPING
0.5120
(
6)
0.2991
Goteo desc
-0.4078
(
6)
0.4222
Olor Putrido desc
0.2200
(
6)
0.6753
Olor Rancio
0.3170
(
6)
0.5404
Olor Rancio desc
0.4815
(
6)
0.3335
GLUTATION PEROXIDASA
-----------------------------------------------------------Olor Tipico
-0.3715
(
6)
0.4684
Olor Tipico desc
-0.2050
(
6)
0.6968
pH
0.4515
(
6)
0.3688
Porcentaje de agua p
-0.5976
(
6)
0.2103
Porcentaje de agua p
-0.1442
(
6)
0.7851
Porcentaje de agua p
0.4296
(
6)
0.3952
Porcentaje de liq pe
-0.2437
(
6)
0.6416
Sabor oxidado
0.3942
(
6)
0.4393
Sabor Putrido
-0.0078
(
6)
0.9883
Sabor Rancio
0.2989
(
6)
0.5650
Sabor tipico
-0.0376
(
6)
0.9437
TIEMPO
0.1806
(
6)
0.7320
-----------------------------------------------------------Correlation
(Sample Size)
P-Value
ANEXO 28
ANALISIS ESTADISTICOS – CORRELACIÓN DE INDICADORES DE
OXIDACION LIPIDICA CON GSH - Px DE MUESTRAS DE INDIVIDUOS
ALIMENTADOS CON DIETA II (EXCESO DE TOCOFEROLES)
Correlations
GLUTATION PEROXIDASA
-----------------------------------------------------------DMA
-0.5554
(
5)
0.3311
HCHO
-0.6181
(
5)
0.2665
INDICE DE PEROXIDOS
-0.2031
(
5)
0.7432
INDICE DE POLIENOS
-0.0640
(
5)
0.9185
NBVT
-0.2215
(
5)
0.7203
P ANISIDINA
-0.2047
(
5)
0.7412
TIEMPO
-0.5171
(
5)
0.3723
-----------------------------------------------------------Correlation
(Sample Size)
P-Value
ANEXO 29
ANALISIS ESTADISTICOS – ANOVA DE UNA VIA PARA GSH – Px DE INDIVIDUOS
ALIMENTADOS CON DIETA III (EXTRACTO DE ROMERO)
ANOVA Table for GLUTATION PEROXIDASA by TIEMPO
Analysis of Variance
----------------------------------------------------------------------------Source
Sum of Squares
Df Mean Square
F-Ratio
P-Value
----------------------------------------------------------------------------Between groups
6.5608E7
5
1.31216E7
4.44
0.0185
Within groups
3.25236E7
11
2.95669E6
----------------------------------------------------------------------------Total (Corr.)
9.81315E7
16
Multiple Range Tests for GLUTATION PEROXIDASA by TIEMPO
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
TIEMPO
Count
Mean
Homogeneous Groups
-------------------------------------------------------------------------------6
2
7858.8
X
3
2
8920.5
XX
9
2
10951.7
XX
18
4
12002.1
XX
0
5
12971.8
X
12
2
14088.7
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------0 - 3
4051.3
4902.63
0 - 6
*5113.0
4902.63
0 - 9
2020.1
4902.63
0 - 12
-1116.9
4902.63
0 - 18
969.725
3930.85
3 - 6
1061.7
5859.76
3 - 9
-2031.2
5859.76
3 - 12
-5168.2
5859.76
3 - 18
-3081.58
5074.7
6 - 9
-3092.9
5859.76
6 - 12
*-6229.9
5859.76
6 - 18
-4143.28
5074.7
9 - 12
-3137.0
5859.76
9 - 18
-1050.38
5074.7
12 - 18
2086.63
5074.7
-------------------------------------------------------------------------------* denotes a statistically significant difference.
GLUTATION PEROXIDASA
Scatterplot by Level Code
15600
13600
11600
9600
7600
5600
0
3
6
9
12
TIEMPO
Summary Statistics for GLUTATION PEROXIDASA
TIEMPO
Count
Average
-----------------------------------------------------------0
5
12971.8
3
2
8920.5
6
2
7858.8
9
2
10951.7
12
2
14088.7
18
4
12002.1
-----------------------------------------------------------Total
17
11559.2
TIEMPO
Variance
Standard deviation
-----------------------------------------------------------0
1.26645E6
1125.37
3
2.13335E7
4618.82
6
2.10905E6
1452.26
9
45722.9
213.829
12
1.78756E6
1337.0
18
727303.0
852.82
-----------------------------------------------------------Total
6.13322E6
2476.53
TIEMPO
Minimum
Maximum
-----------------------------------------------------------0
11453.5
14510.9
3
5654.5
12186.5
6
6831.9
8885.7
9
10800.5
11102.9
12
13143.3
15034.1
18
11206.0
13149.8
-----------------------------------------------------------Total
5654.5
15034.1
TIEMPO
Range
Stnd. skewness
-----------------------------------------------------------0
3057.4
0.0202948
3
6532.0
6
2053.8
9
302.4
12
1890.8
18
1943.8
0.796247
-----------------------------------------------------------Total
9379.6
-1.92464
18
TIEMPO
Stnd. kurtosis
-----------------------------------------------------------0
0.262889
3
6
9
12
18
0.108222
-----------------------------------------------------------Total
1.03245
Table of Means for GLUTATION PEROXIDASA by TIEMPO
with 95.0 percent Tukey HSD intervals
-------------------------------------------------------------------------------Stnd. error
TIEMPO
Count
Mean
(pooled s)
Lower limit
Upper limit
-------------------------------------------------------------------------------0
5
12971.8
768.985
11118.8
14824.8
3
2
8920.5
1215.87
5990.62
11850.4
6
2
7858.8
1215.87
4928.92
10788.7
9
2
10951.7
1215.87
8021.82
13881.6
12
2
14088.7
1215.87
11158.8
17018.6
18
4
12002.1
859.751
9930.34
14073.8
-------------------------------------------------------------------------------Total
17
11559.2
Variance Check
Cochran's C test: 0.782319
P-Value = 0.0046584
Bartlett's test: 2.62907
P-Value = 0.146468
Hartley's test: 466.583
Kruskal-Wallis Test for GLUTATION PEROXIDASA by TIEMPO
TIEMPO
Sample Size
Average Rank
-----------------------------------------------------------0
5
12.2
3
2
5.5
6
2
2.5
9
2
4.5
12
2
15.0
18
4
9.25
-----------------------------------------------------------Test statistic = 10.7039
P-Value = 0.0575768
ANEXO 30
ANALISIS ESTADISTICOS – CORRELACIÓN DE PROPIEDADES FISICAS Y
SENSORIALES CON GSH - Px DE MUESTRAS DE INDIVIDUOS ALIMENTADOS
CON LA DIETA III (EXTRACTO DE ROMERO)
Correlations
GLUTATION PEROXIDASA
-----------------------------------------------------------CFU Ent
0.6406
(
6)
0.1705
CFU Prom RAM
-0.0435
(
6)
0.9349
Cohesividad Ciz Coc
-0.0972
(
6)
0.8546
Cohesividad Ciz Crud
-0.2739
(
6)
0.5994
Cohesividad Compresi
-0.2423
(
6)
0.6437
Color
-0.7819
(
6)
0.0662
Color Roche desc
-0.4984
(
6)
0.3143
Deshidratacion desc
0.3912
(
6)
0.4431
Dureza N Ciz Coc
-0.6108
(
6)
0.1977
Dureza N Ciz Cruda
0.0901
(
6)
0.8652
Dureza N Compresion
-0.3712
(
6)
0.4688
Elasticidad desc
-0.1617
(
6)
0.7595
GAPING
0.4298
(
6)
0.3949
Goteo desc
0.2809
(
6)
0.5898
Olor Putrido desc
0.4033
(
6)
0.4278
Olor Rancio
0.5007
(
6)
0.3117
Olor Rancio desc
0.3140
(
6)
0.5444
Olor Tipico
-0.2820
(
6)
0.5882
GLUTATION PEROXIDASA
-----------------------------------------------------------Olor Tipico desc
-0.2701
(
6)
0.6046
pH
0.6289
(
6)
0.1811
Porcentaje de agua p
-0.5377
(
6)
0.2712
Porcentaje de agua p
0.4891
(
6)
0.3248
Porcentaje de agua p
0.0288
(
6)
0.9569
Porcentaje de liq pe
0.2190
(
6)
0.6767
Sabor oxidado
0.4456
(
6)
0.3759
Sabor Putrido
0.6296
(
6)
0.1804
Sabor Rancio
0.5109
(
6)
0.3004
Sabor tipico
-0.3285
(
6)
0.5249
TIEMPO
0.3725
(
6)
0.4670
-----------------------------------------------------------Correlation
(Sample Size)
P-Value
ANEXO 31
ANALISIS ESTADISTICOS – CORRELACIÓN DE INDICADORES DE OXIDACION
LIPIDICA CON GSH - Px DE MUESTRAS DE INDIVIDUOS ALIMENTADOS CON
DIETA III (EXTRACTO DE ROMERO)
Correlations
GLUTATION PEROXIDASA
-----------------------------------------------------------DMA
-0.3162
(
5)
0.6042
HCHO
-0.1078
(
5)
0.8630
INDICE DE PEROXIDOS
0.0009
(
5)
0.9989
INDICE DE POLIENOS
0.5262
(
5)
0.3623
NBVT
-0.0256
(
5)
0.9674
P ANISIDINA
0.4651
(
5)
0.4299
TIEMPO
0.2567
(
5)
0.6768
-----------------------------------------------------------Correlation
(Sample Size)
P-Value
ANEXO 32
ANALISIS ESTADISTICOS - ANOVA DE UNA VIA PARA GSH - Px DE MUESTRAS
DE INDIVIDUOS ALIMENTADOS CON LAS TRES DIETAS, PARA CADA UNO DE
LOS TIEMPOS ANALIZADOS
Mes 0 de almacenamiento
ANOVA Table for GLUTATION PEROXIDASA by DIETA
Analysis of Variance
----------------------------------------------------------------------------Source
Sum of Squares
Df Mean Square
F-Ratio
P-Value
----------------------------------------------------------------------------Between groups
1.54782E8
2
7.7391E7
7.25
0.0086
Within groups
1.28069E8
12
1.06724E7
----------------------------------------------------------------------------Total (Corr.)
2.82851E8
14
GLUTATION PEROXIDASA
Multiple Range Tests for GLUTATION PEROXIDASA by DIETA
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
DIETA
Count
Mean
Homogeneous Groups
-------------------------------------------------------------------------------1
5
8510.1
X
3
5
12971.8
XX
2
5
16353.8
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------1 - 2
*-7843.74
5527.35
1 - 3
-4461.7
5527.35
2 - 3
3382.04
5527.35
-------------------------------------------------------------------------------* denotes a statistically significant difference.
Scatterplot by Level Code
(X 1000)
24
20
16
12
8
4
0
1
2
DIETA
3
Summary Statistics for GLUTATION PEROXIDASA
DIETA
Count
Average
-----------------------------------------------------------1
5
8510.1
2
5
16353.8
3
5
12971.8
-----------------------------------------------------------Total
15
12611.9
DIETA
Variance
Standard deviation
-----------------------------------------------------------1
2.00368E7
4476.25
2
1.07139E7
3273.21
3
1.26645E6
1125.37
-----------------------------------------------------------Total
2.02036E7
4494.84
DIETA
Minimum
Maximum
-----------------------------------------------------------1
5296.6
16085.3
2
12271.1
20748.4
3
11453.5
14510.9
-----------------------------------------------------------Total
5296.6
20748.4
DIETA
Range
Stnd. skewness
-----------------------------------------------------------1
10788.7
1.57632
2
8477.3
0.173197
3
3057.4
0.0202948
-----------------------------------------------------------Total
15451.8
-0.279541
DIETA
Stnd. kurtosis
-----------------------------------------------------------1
1.28544
2
-0.300813
3
0.262889
-----------------------------------------------------------Total
-0.342712
Table of Means for GLUTATION PEROXIDASA by DIETA
with 95.0 percent Tukey HSD intervals
-------------------------------------------------------------------------------Stnd. error
DIETA
Count
Mean
(pooled s)
Lower limit
Upper limit
-------------------------------------------------------------------------------1
5
8510.1
1460.99
5746.43
11273.8
2
5
16353.8
1460.99
13590.2
19117.5
3
5
12971.8
1460.99
10208.1
15735.5
-------------------------------------------------------------------------------Total
15
12611.9
Variance Check
Cochran's C test: 0.625814
P-Value = 0.206036
Bartlett's test: 1.64743
P-Value = 0.0674906
Hartley's test: 15.8212
Mes 3 de almacenamiento
ANOVA Table for GLUTATION PEROXIDASA by DIETA
Analysis of Variance
----------------------------------------------------------------------------Source
Sum of Squares
Df Mean Square
F-Ratio
P-Value
----------------------------------------------------------------------------Between groups
4.22136E6
2
2.11068E6
0.28
0.7756
Within groups
2.28675E7
3
7.62252E6
----------------------------------------------------------------------------Total (Corr.)
2.70889E7
5
GLUTATION PEROXIDASA
Multiple Range Tests for GLUTATION PEROXIDASA by DIETA
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
DIETA
Count
Mean
Homogeneous Groups
-------------------------------------------------------------------------------3
2
8920.5
X
1
2
10560.7
X
2
2
10812.2
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------1 - 2
-251.5
11527.9
1 - 3
1640.2
11527.9
2 - 3
1891.7
11527.9
-------------------------------------------------------------------------------* denotes a statistically significant difference.
Scatterplot by Level Code
13600
11600
9600
7600
5600
1
2
DIETA
3
Summary Statistics for GLUTATION PEROXIDASA
DIETA
Count
Average
-----------------------------------------------------------1
2
10560.7
2
2
10812.2
3
2
8920.5
-----------------------------------------------------------Total
6
10097.8
DIETA
Variance
Standard deviation
-----------------------------------------------------------1
1.50581E6
1227.11
2
28226.9
168.009
3
2.13335E7
4618.82
-----------------------------------------------------------Total
5.41778E6
2327.61
DIETA
Minimum
Maximum
-----------------------------------------------------------1
9693.0
11428.4
2
10693.4
10931.0
3
5654.5
12186.5
-----------------------------------------------------------Total
5654.5
12186.5
DIETA
Range
Stnd. skewness
-----------------------------------------------------------1
1735.4
2
237.6
3
6532.0
-----------------------------------------------------------Total
6532.0
-1.79689
DIETA
Stnd. kurtosis
-----------------------------------------------------------1
2
3
-----------------------------------------------------------Total
1.7947
Table of Means for GLUTATION PEROXIDASA by DIETA
with 95.0 percent Tukey HSD intervals
-------------------------------------------------------------------------------Stnd. error
DIETA
Count
Mean
(pooled s)
Lower limit
Upper limit
-------------------------------------------------------------------------------1
2
10560.7
1952.24
4796.73
16324.7
2
2
10812.2
1952.24
5048.23
16576.2
3
2
8920.5
1952.24
3156.53
14684.5
-------------------------------------------------------------------------------Total
6
10097.8
Variance Check
Cochran's C test: 0.932917
P-Value = 0.102372
Bartlett's test: 7.87529
P-Value = 0.11729
Hartley's test: 755.787
Kruskal-Wallis Test for GLUTATION PEROXIDASA by DIETA
DIETA
Sample Size
Average Rank
-----------------------------------------------------------1
2
3.5
2
2
3.5
3
2
3.5
-----------------------------------------------------------Test statistic = 0.0
P-Value = 1.0
Mes 6 de almacenamiento
ANOVA Table for GLUTATION PEROXIDASA by DIETA
Analysis of Variance
----------------------------------------------------------------------------Source
Sum of Squares
Df Mean Square
F-Ratio
P-Value
----------------------------------------------------------------------------Between groups
4.40276E7
2
2.20138E7
17.89
0.0215
Within groups
3.69094E6
3
1.23031E6
----------------------------------------------------------------------------Total (Corr.)
4.77185E7
5
GLUTATION PEROXIDASA
Multiple Range Tests for GLUTATION PEROXIDASA by DIETA
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
DIETA
Count
Mean
Homogeneous Groups
-------------------------------------------------------------------------------3
2
7858.8
X
2
2
12653.2
X
1
2
14228.5
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------1 - 2
1575.4
4631.38
1 - 3
*6369.75
4631.38
2 - 3
*4794.35
4631.38
-------------------------------------------------------------------------------* denotes a statistically significant difference.
Scatterplot by Level Code
16800
14800
12800
10800
8800
6800
1
2
DIETA
3
Summary Statistics for GLUTATION PEROXIDASA
DIETA
Count
Average
-----------------------------------------------------------1
2
14228.5
2
2
12653.2
3
2
7858.8
-----------------------------------------------------------Total
6
11580.2
DIETA
Variance
Standard deviation
-----------------------------------------------------------1
1.43059E6
1196.07
2
151305.0
388.979
3
2.10905E6
1452.26
-----------------------------------------------------------Total
9.54371E6
3089.29
DIETA
Minimum
Maximum
-----------------------------------------------------------1
13382.8
15074.3
2
12378.1
12928.2
3
6831.9
8885.7
-----------------------------------------------------------Total
6831.9
15074.3
DIETA
Range
Stnd. skewness
-----------------------------------------------------------1
1691.5
2
550.1
3
2053.8
-----------------------------------------------------------Total
8242.4
-0.764587
DIETA
Stnd. kurtosis
-----------------------------------------------------------1
2
3
-----------------------------------------------------------Total
-0.341522
Table of Means for GLUTATION PEROXIDASA by DIETA
with 95.0 percent Tukey HSD intervals
-------------------------------------------------------------------------------Stnd. error
DIETA
Count
Mean
(pooled s)
Lower limit
Upper limit
-------------------------------------------------------------------------------1
2
14228.5
784.319
11912.9
16544.2
2
2
12653.2
784.319
10337.5
14968.8
3
2
7858.8
784.319
5543.11
10174.5
-------------------------------------------------------------------------------Total
6
11580.2
Variance Check
Cochran's C test: 0.571412
P-Value = 0.732245
Bartlett's test: 1.59783
P-Value = 0.614668
Hartley's test: 13.939
Kruskal-Wallis Test for GLUTATION PEROXIDASA by DIETA
DIETA
Sample Size
Average Rank
-----------------------------------------------------------1
2
5.5
2
2
3.5
3
2
1.5
-----------------------------------------------------------Test statistic = 4.57143
P-Value = 0.101701
Mes 9 de almacenamiento
ANOVA Table for GLUTATION PEROXIDASA by DIETA
Analysis of Variance
----------------------------------------------------------------------------Source
Sum of Squares
Df Mean Square
F-Ratio
P-Value
----------------------------------------------------------------------------Between groups
4.72564E6
2
2.36282E6
1.73
0.3160
Within groups
4.09014E6
3
1.36338E6
----------------------------------------------------------------------------Total (Corr.)
8.81577E6
5
GLUTATION PEROXIDASA
Multiple Range Tests for GLUTATION PEROXIDASA by DIETA
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
DIETA
Count
Mean
Homogeneous Groups
-------------------------------------------------------------------------------3
2
10951.7
X
1
2
12309.8
X
2
2
13100.8
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------1 - 2
-790.95
4875.41
1 - 3
1358.1
4875.41
2 - 3
2149.05
4875.41
-------------------------------------------------------------------------------*denotes a statistically significant difference.
Scatterplot by Level Code
(X 1000)
14
13
12
11
10
1
2
DIETA
3
Summary Statistics for GLUTATION PEROXIDASA
DIETA
Count
Average
-----------------------------------------------------------1
2
12309.8
2
2
13100.8
3
2
10951.7
-----------------------------------------------------------Total
6
12120.8
DIETA
Variance
Standard deviation
-----------------------------------------------------------1
4.03507E6
2008.75
2
9343.45
96.6615
3
45722.9
213.829
-----------------------------------------------------------Total
1.76315E6
1327.84
DIETA
Minimum
Maximum
-----------------------------------------------------------1
10889.4
13730.2
2
13032.4
13169.1
3
10800.5
11102.9
-----------------------------------------------------------Total
10800.5
13730.2
DIETA
Range
Stnd. skewness
-----------------------------------------------------------1
2840.8
2
136.7
3
302.4
-----------------------------------------------------------Total
2929.7
0.109697
DIETA
Stnd. kurtosis
-----------------------------------------------------------1
2
3
-----------------------------------------------------------Total
-1.43407
Table of Means for GLUTATION PEROXIDASA by DIETA
with 95.0 percent Tukey HSD intervals
-------------------------------------------------------------------------------Stnd. error
DIETA
Count
Mean
(pooled s)
Lower limit
Upper limit
-------------------------------------------------------------------------------1
2
12309.8
825.645
9872.09
14747.5
2
2
13100.8
825.645
10663.0
15538.5
3
2
10951.7
825.645
8513.99
13389.4
-------------------------------------------------------------------------------Total
6
12120.8
Variance Check
Cochran's C test: 0.986537
P-Value = 0.0202632
Bartlett's test: 11.3707
P-Value = 0.0800952
Hartley's test: 431.861
Kruskal-Wallis Test for GLUTATION PEROXIDASA by DIETA
DIETA
Sample Size
Average Rank
-----------------------------------------------------------1
2
4.0
2
2
4.5
3
2
2.0
-----------------------------------------------------------Test statistic = 2.0
P-Value = 0.367879
Mes 12 de almacenamiento
ANOVA Table for GLUTATION PEROXIDASA by DIETA
Analysis of Variance
----------------------------------------------------------------------------Source
Sum of Squares
Df Mean Square
F-Ratio
P-Value
----------------------------------------------------------------------------Between groups
8.05623E6
2
4.02811E6
5.33
0.1029
Within groups
2.26597E6
3
755323.0
----------------------------------------------------------------------------Total (Corr.)
1.03222E7
5
GLUTATION PEROXIDASA
Multiple Range Tests for GLUTATION PEROXIDASA by DIETA
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
DIETA
Count
Mean
Homogeneous Groups
-------------------------------------------------------------------------------1
2
11507.8
X
2
2
11775.3
X
3
2
14088.7
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------1 - 2
-267.4
3628.85
1 - 3
-2580.85
3628.85
2 - 3
-2313.45
3628.85
-------------------------------------------------------------------------------*denotes a statistically significant difference.
Scatterplot by Level Code
(X 1000)
16
15
14
13
12
11
1
2
DIETA
3
Summary Statistics for GLUTATION PEROXIDASA
DIETA
Count
Average
-----------------------------------------------------------1
2
11507.8
2
2
11775.3
3
2
14088.7
-----------------------------------------------------------Total
6
12457.3
DIETA
Variance
Standard deviation
-----------------------------------------------------------1
24398.4
156.2
2
454009.0
673.802
3
1.78756E6
1337.0
-----------------------------------------------------------Total
2.06444E6
1436.82
DIETA
Minimum
Maximum
-----------------------------------------------------------1
11397.4
11618.3
2
11298.8
12251.7
3
13143.3
15034.1
-----------------------------------------------------------Total
11298.8
15034.1
DIETA
Range
Stnd. skewness
-----------------------------------------------------------1
220.9
2
952.9
3
1890.8
-----------------------------------------------------------Total
3735.3
1.42492
DIETA
Stnd. kurtosis
-----------------------------------------------------------1
2
3
-----------------------------------------------------------Total
0.806397
Table of Means for GLUTATION PEROXIDASA by DIETA
with 95.0 percent Tukey HSD intervals
-------------------------------------------------------------------------------Stnd. error
DIETA
Count
Mean
(pooled s)
Lower limit
Upper limit
-------------------------------------------------------------------------------1
2
11507.8
614.542
9693.42
13322.3
2
2
11775.3
614.542
9960.82
13589.7
3
2
14088.7
614.542
12274.3
15903.1
-------------------------------------------------------------------------------Total
6
12457.3
Variance Check
Cochran's C test: 0.788873
P-Value = 0.335444
Bartlett's test: 2.79193
P-Value = 0.344307
Hartley's test: 73.2655
Kruskal-Wallis Test for GLUTATION PEROXIDASA by DIETA
DIETA
Sample Size
Average Rank
-----------------------------------------------------------1
2
2.5
2
2
2.5
3
2
5.5
-----------------------------------------------------------Test statistic = 3.42857
P-Value = 0.180092
Mes 18 de almacenamiento
ANOVA Table for GLUTATION PEROXIDASA by DIETA
Analysis of Variance
----------------------------------------------------------------------------Source
Sum of Squares
Df Mean Square
F-Ratio
P-Value
----------------------------------------------------------------------------Between groups
2.30606E7
2
1.15303E7
1.08
0.3784
Within groups
9.56664E7
9
1.06296E7
----------------------------------------------------------------------------Total (Corr.)
1.18727E8
11
GLUTATION PEROXIDASA
Multiple Range Tests for GLUTATION PEROXIDASA by DIETA
-------------------------------------------------------------------------------Method: 95.0 percent Tukey HSD
DIETA
Count
Mean
Homogeneous Groups
-------------------------------------------------------------------------------3
4
12002.1
X
1
4
14094.1
X
2
4
15364.4
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------1 - 2
-1270.3
6435.92
1 - 3
2092.02
6435.92
2 - 3
3362.32
6435.92
-------------------------------------------------------------------------------*denotes a statistically significant difference.
Scatterplot by Level Code
(X 1000)
22
19
16
13
10
7
1
2
DIETA
3
Summary Statistics for GLUTATION PEROXIDASA
DIETA
Count
Average
-----------------------------------------------------------1
4
14094.1
2
4
15364.4
3
4
12002.1
-----------------------------------------------------------Total
12
13820.2
DIETA
Variance
Standard deviation
-----------------------------------------------------------1
2.10382E7
4586.74
2
1.01233E7
3181.72
3
727303.0
852.82
-----------------------------------------------------------Total
1.07934E7
3285.33
DIETA
Minimum
Maximum
-----------------------------------------------------------1
7316.2
17278.4
2
12871.3
19871.9
3
11206.0
13149.8
-----------------------------------------------------------Total
7316.2
19871.9
DIETA
Range
Stnd. skewness
-----------------------------------------------------------1
9962.2
-1.4913
2
7000.6
1.15895
3
1943.8
0.796247
-----------------------------------------------------------Total
12555.7
-0.102064
DIETA
Stnd. kurtosis
-----------------------------------------------------------1
1.39264
2
0.678287
3
0.108222
-----------------------------------------------------------Total
0.413157
Table of Means for GLUTATION PEROXIDASA by DIETA
with 95.0 percent Tukey HSD intervals
-------------------------------------------------------------------------------Stnd. error
DIETA
Count
Mean
(pooled s)
Lower limit
Upper limit
-------------------------------------------------------------------------------1
4
14094.1
1630.15
10876.1
17312.1
2
4
15364.4
1630.15
12146.4
18582.4
3
4
12002.1
1630.15
8784.12
15220.0
-------------------------------------------------------------------------------Total
12
13820.2
Variance Check
Cochran's C test: 0.659735
P-Value = 0.22291
Bartlett's test: 1.97925
P-Value = 0.0688503
Hartley's test: 28.9263
Kruskal-Wallis Test for GLUTATION PEROXIDASA by DIETA
DIETA
Sample Size
Average Rank
-----------------------------------------------------------1
4
7.75
2
4
8.0
3
4
3.75
-----------------------------------------------------------Test statistic = 3.5
P-Value = 0.173774
ANEXO 33
ANALISIS COMPUTACIONAL DE LA VARIACION DE LA ACTIVIDAD MMP’s
A.- Dieta I o Control
Mes 0
Mes 3
Mes 6
Pixel total
B1
81744,51
B2
42772
B1
140944,5
B2
51197
B1
147898
B2
111422,5
Background
31123,84
15510,08
49875,18
18703,19
68187,5
51076,57
Pix Total
50620,67
27261,92
91069,32
32493,81
79710,5
60345,91
Pix Total %
65
35
73,7
26,3
56,91
43,09
Y Centroid
205
396
205
395
205
395
Mol weight
0
0
0
0
0
0
848
424
1368
513
1652
1239
Seg Area %
66,67
33,33
72,73
27,27
57,14
42,86
Max Pixel
64,26
65,53
76,49
64,8
59,27
57,52
Avg Pixel
59,69
64,3
66,57
63,34
48,25
48,71
Seg Area
Mes 9
Mes 12
Mes 18
B1
212818
B2
120239
B1
160028,5
B2
170399
B1
174186,5
258251,5
Background
97958,37
50793,23
71218,94
71107,93
83061,28
125867,9
Pix Total
114859,7
69445,78
88809,59
99291,05
91125,21
132383,5
Pix Total %
62,32
37,68
47,21
52,79
40,77
59,23
Y Centroid
205
398
206
401
207
403
Mol weight
0
0
0
0
0
0
Seg Area
1782
924
1560
1560
1470
2240
Seg Area %
65,85
34,15
50
50
39,62
60,38
Max Pixel
75,56
82,48
69,35
81,65
69,58
85,49
Avg Pixel
64,46
75,16
56,93
63,65
61,99
59,1
Pixel total
B2
B.- Dieta II (Exceso de Tocoferoles)
Mes 0
Mes 3
Mes 6
B1
74075
B2
-
B1
191128,5
B2
-
B1
90917
B2
-
Background
42836,45
-
109799,2
-
53742,45
-
Pix Total
31238,55
-
81329,34
-
37174,55
-
Pix Total %
100
-
100
-
100
-
Y Centroid
204
-
196
-
198
-
Mol weight
0
-
0
-
0
-
Seg Area
885
-
2562
-
1008
-
Seg Area %
100
-
100
-
100
-
Max Pixel
36,46
-
36,11
-
40,22
-
Avg Pixel
35,3
-
31,74
-
36,88
-
Pixel total
Mes 9
Mes 12
Mes 18
B1
82026
B2
-
B1
127337,5
B2
158085
B1
174673
119892
Background
39809,12
-
64959,53
80345,74
93273,33
49264,21
Pix Total
42216,88
-
62377,96
77739,29
81399,67
70627,8
Pix Total %
100
-
44,52
55,48
53,54
46,46
Y Centroid
77
-
195
393
192
381
Mol weight
0
-
0
0
0
0
Seg Area
780
-
1134
1404
1944
1026
Seg Area %
100
-
44,68
55,32
65,45
34,55
Max Pixel
57,64
-
60,44
62,42
48,32
70,02
Avg Pixel
54,12
-
55,01
55,37
41,87
68,84
Pixel total
B2
C.- Dieta III (Extracto de Romero)
Mes 0
Mes 3
Mes 6
Pixel total
B1
110247,5
B2
61110,5
B1
38694
B2
70813,5
B1
89127,5
B2
82412,51
Background
46096,41
21835,14
18903,76
24554
45109,52
34495,52
Pix Total
64151,07
39275,36
19790,24
46259,5
44017,98
47916,99
Pix Total %
62,03
37,97
29,69
70,04
47,88
52,12
Y Centroid
63
236
63
237
63
245
Mol weight
0
0
0
0
0
0
Seg Area
1102
522
460
598
884
676
Seg Area %
67,86
32,14
43,48
56,52
56,67
43,33
Max Pixel
63,95
76,14
44,19
78,57
54,43
72,05
Avg Pixel
58,21
75,24
43,02
77,36
49,79
70,88
Mes 9
Mes 12
Mes 18
B1
77936
B2
107230,5
B1
132058
B2
105921
B1
62262,5
83867,01
Background
41973,7
41973,7
51679,06
45219,18
26299,24
26299,24
Pix Total
35962,3
65256,8
80378,95
60701,82
35963,26
57567,77
Pix Total %
35,53
64,47
56,97
43,03
38,45
61,55
Y Centroid
63
246
63
248
60
242
Mol weight
0
0
0
0
0
0
Seg Area
825
825
864
756
612
612
Seg Area %
50
50
53,33
46,67
50
50
Max Pixel
46,16
82,61
97,91
85,43
60,85
96,58
Avg Pixel
43,59
79,1
93,03
80,29
58,76
94,06
Pixel total
B2
ANEXO 34
ANALISIS ESTADISTICO ANOVA MULTIFACTORIAL DEL COMPORTAMIENTO
DE MMP’s CON RESPECTO AL TIEMPO
™ Banda 1
Analysis of Variance for Banda 1 - Type III Sums of Squares
-------------------------------------------------------------------------------Source
Sum of Squares
Df
Mean Square
F-Ratio
P-Value
-------------------------------------------------------------------------------MAIN EFFECTS
A:Tiempo
2408,19
5
481,638
2,07
0,1405
RESIDUAL
2795,79
12
232,983
-------------------------------------------------------------------------------TOTAL (CORRECTED)
5203,98
17
-------------------------------------------------------------------------------All F-ratios are based on the residual mean square error.
Multiple Range Tests for Banda 1 by Tiempo
-------------------------------------------------------------------------------Method: 95,0 percent Tukey HSD
Tiempo
Count
LS Mean
Homogeneous Groups
-------------------------------------------------------------------------------3
3
87,2494
X
0
3
93,3802
X
6
3
93,515
X
18
3
103,361
X
9
3
106,352
X
12
3
122,573
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------0 - 3
6,13074
41,8673
0 - 6
-0,134846
41,8673
0 - 9
-12,9718
41,8673
0 - 12
-29,1924
41,8673
0 - 18
-9,98073
41,8673
3 - 6
-6,26558
41,8673
3 - 9
-19,1025
41,8673
3 - 12
-35,3232
41,8673
3 - 18
-16,1115
41,8673
6 - 9
-12,837
41,8673
6 - 12
-29,0576
41,8673
6 - 18
-9,84589
41,8673
9 - 12
-16,2206
41,8673
9 - 18
2,99107
41,8673
12 - 18
19,2117
41,8673
-------------------------------------------------------------------------------* denotes a statistically significant difference.
Scatterplot by Level Code
154
Banda 1
134
114
94
74
0
3
6
9
12
18
Tiempo
™ Banda 2
Analysis of Variance for Banda 2 - Type III Sums of Squares
-------------------------------------------------------------------------------Source
Sum of Squares
Df
Mean Square
F-Ratio
P-Value
-------------------------------------------------------------------------------MAIN EFFECTS
A:Tiempo
9032,87
5
1806,57
0,60
0,7031
RESIDUAL
36292,5
12
3024,37
-------------------------------------------------------------------------------TOTAL (CORRECTED)
45325,3
17
-------------------------------------------------------------------------------All F-ratios are based on the residual mean square error.
Multiple Range Tests for Banda 2 by Tiempo
-------------------------------------------------------------------------------Method: 95,0 percent Tukey HSD
Tiempo
Count
LS Mean
Homogeneous Groups
-------------------------------------------------------------------------------6
3
70,6138
X
0
3
72,6491
X
3
3
72,7388
X
9
3
86,7017
X
12
3
120,644
X
18
3
123,061
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------0 - 3
-0,0897208
150,845
0 - 6
2,0353
150,845
0 - 9
-14,0526
150,845
0 - 12
-47,9954
150,845
0 - 18
-50,4117
150,845
3 - 6
2,12502
150,845
3 - 9
-13,9629
150,845
3 - 12
-47,9057
150,845
3 - 18
-50,3219
150,845
6 - 9
-16,0879
150,845
6 - 12
-50,0307
150,845
6 - 18
-52,447
150,845
9 - 12
-33,9428
150,845
9 - 18
-36,359
150,845
12 - 18
-2,41627
150,845
-------------------------------------------------------------------------------* denotes a statistically significant difference.
Scatterplot by Level Code
150
Banda 2
120
90
60
30
0
0
3
6
9
Tiempo
12
18
ANEXO 35
ANALISIS ESTADISTICO ANOVA MULTIFACTORIAL DEL COMPORTAMIENTO
DE MMP’s CON RESPECTO A LAS DIETAS
™ Banda 1
Analysis of Variance for Banda 1 - Type III Sums of Squares
-------------------------------------------------------------------------------Source
Sum of Squares
Df
Mean Square
F-Ratio
P-Value
-------------------------------------------------------------------------------MAIN EFFECTS
A:Dieta
642,552
2
321,276
1,06
0,3722
RESIDUAL
4561,43
15
304,095
-------------------------------------------------------------------------------TOTAL (CORRECTED)
5203,98
17
-------------------------------------------------------------------------------All F-ratios are based on the residual mean square error.
Multiple Range Tests for Banda 1 by Dieta
-------------------------------------------------------------------------------Method: 95,0 percent Tukey HSD
Dieta
Count
LS Mean
Homogeneous Groups
-------------------------------------------------------------------------------2
6
92,6336
X
1
6
104,909
X
3
6
105,672
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------1 - 2
12,2757
26,2624
1 - 3
-0,762702
26,2624
2 - 3
-13,0384
26,2624
-------------------------------------------------------------------------------* denotes a statistically significant difference.
Scatterplot by Level Code
154
Banda 1
134
114
94
74
1
2
Dieta
3
™ Banda 2
Analysis of Variance for Banda 2 - Type III Sums of Squares
-------------------------------------------------------------------------------Source
Sum of Squares
Df
Mean Square
F-Ratio
P-Value
-------------------------------------------------------------------------------MAIN EFFECTS
A:Dieta
26301,0
2
13150,5
10,37
0,0015
RESIDUAL
19024,3
15
1268,29
-------------------------------------------------------------------------------TOTAL (CORRECTED)
45325,3
17
-------------------------------------------------------------------------------All F-ratios are based on the residual mean square error.
Multiple Range Tests for Banda 2 by Dieta
-------------------------------------------------------------------------------Method: 95,0 percent Tukey HSD
Dieta
Count
LS Mean
Homogeneous Groups
-------------------------------------------------------------------------------2
6
38,2417
X
1
6
107,543
X
3
6
127,42
X
-------------------------------------------------------------------------------Contrast
Difference
+/- Limits
-------------------------------------------------------------------------------1 - 2
*69,301
53,6338
1 - 3
-19,8774
53,6338
2 - 3
*-89,1784
53,6338
-------------------------------------------------------------------------------* denotes a statistically significant difference.
Scatterplot by Level Code
150
Banda 2
120
90
60
30
0
1
2
Dieta
3
ANEXO 36
ANALISIS ESTADISTICO CORRELACIÓN DE LA VARIACIÓN DE MMP’s CON
PARAMETRO DE CALIDAD DEL SALMÓN
A.- Dieta I o Control
Correlations
Banda 1
Banda 2
-----------------------------------------------------Banda 1
0,9191
(
6)
0,0096
Banda 2
0,9191
(
6)
0,0096
CFU Ent
-0,5261
-0,4573
(
6)
(
6)
0,2836
0,3618
CFU Prom RAM
-0,4410
-0,3825
(
6)
(
6)
0,3814
0,4542
Cohesividad Ciz coc -0,0070
0,2559
(
6)
(
6)
0,9895
0,6246
Cohesividad Ciz crud-0,4456
-0,0959
(
6)
(
6)
0,3758
0,8567
Cohesividad Compresi-0,6298
-0,3835
(
6)
(
6)
0,1802
0,4530
Color
0,1957
-0,0541
(
6)
(
6)
0,7102
0,9189
Color Roche desc
-0,8684
-0,8872
(
6)
(
6)
0,0248
0,0184
Deshidratacion desc 0,4902
0,4250
(
6)
(
6)
0,3236
0,4009
Dureza N Ciz cocida -0,0667
0,2001
(
6)
(
6)
0,9002
0,7038
Dureza N Ciz Cruda
0,8670
0,8182
(
6)
(
6)
0,0253
0,0466
Dureza N compresion -0,3205
-0,0215
(
6)
(
6)
0,5357
0,9678
Elasticidad desc
-0,4364
-0,4237
(
6)
(
6)
0,3869
0,4025
Gaping
0,7958
0,9396
(
6)
(
6)
0,0583
0,0054
Goteo desc
0,5207
0,4124
(
6)
(
6)
0,2895
0,4164
Correlations
Banda 1
Banda 2
-----------------------------------------------------Olor putrido desc
0,5560
0,2802
(
6)
(
6)
0,2519
0,5907
Olor rancio
0,3904
0,1905
(
6)
(
6)
0,4441
0,7176
Olor rancio desc
0,4725
0,2538
(
6)
(
6)
0,3441
0,6275
Olor tipico
-0,4512
-0,3415
(
6)
(
6)
0,3691
0,5077
Olor tipico desc
-0,5586
-0,3380
(
6)
(
6)
0,2493
0,5123
pH
-0,2179
-0,3755
(
6)
(
6)
0,6784
0,4632
Porcentaje agua perd-0,6640
-0,3443
(Dp coc)
(
6)
(
6)
0,1504
0,5039
Porcentaje agua perd 0,5782
0,4901
(CRA)
(
6)
(
6)
0,2293
0,3237
Porcentaje de agua p 0,0216
0,1228
(Hum)
(
6)
(
6)
0,9677
0,8168
Porcentaje Liq perdi-0,9447
-0,9013
(Dp cru)
(
6)
(
6)
0,0045
0,0141
Sabor oxidado
0,5475
0,3438
(
6)
(
6)
0,2608
0,5046
Sabor putrido
0,5713
0,3435
(
6)
(
6)
0,2362
0,5050
Sabor rancio
0,5907
0,3328
(
6)
(
6)
0,2170
0,5192
Sabor tipico
-0,3831
-0,2363
(
6)
(
6)
0,4535
0,6521
Tiempo
0,6442
0,5140
(
6)
(
6)
0,1674
0,2969
-----------------------------------------------------------Correlation
(Sample Size)
P-Value
B.- Dieta II (Exceso de Tocoferoles)
Correlations
Banda 1
Banda 2
---------------------------------------------------------Banda 1
0,4595
(
6)
0,3593
Banda 2
0,4595
(
6)
0,3593
CFU Ent
-0,8723
-0,3395
(
6)
(
6)
0,0234
0,5104
CFU Prom RAM
0,2889
-0,3284
(
6)
(
6)
0,5787
0,5250
Cohesividad Ciz coc 0,7651
0,3904
(
6)
(
6)
0,0763
0,4441
Cohesividad Ciz crud 0,1867
-0,6683
(
6)
(
6)
0,7232
0,1468
Cohesividad Compresi 0,1197
(
6)
0,8213
Color
-0,6660
(
6)
0,1487
Color Roche desc
-0,6250
(
6)
0,1846
Deshidratacion desc 0,7970
(
6)
0,0577
Dureza N Ciz cocida 0,3466
(
6)
0,5009
Dureza N Ciz Cruda
0,1591
(
6)
0,7634
Dureza N compresion 0,2528
(
6)
0,6289
Elasticidad desc
-0,6068
(
6)
0,2015
Gaping
0,1960
(
6)
0,7097
Goteo desc
0,7902
(
6)
0,0614
Olor putrido desc
0,5918
(
6)
0,2159
Olor rancio
0,4958
(
6)
0,3172
Olor rancio desc
0,0119
(
6)
0,9821
-0,6386
(
6)
0,1723
-0,1551
(
6)
0,7692
-0,4736
(
6)
0,3427
0,6816
(
6)
0,1360
-0,4121
(
6)
0,4168
0,2700
(
6)
0,6049
-0,4974
(
6)
0,3154
-0,7277
(
6)
0,1012
0,7637
(
6)
0,0771
0,5995
(
6)
0,2084
0,4409
(
6)
0,3815
0,9240
(
6)
0,0084
0,6590
(
6)
0,1546
Correlations
Banda 1
Banda 2
---------------------------------------------------------Olor tipico
-0,1264
-0,6778
(
6)
(
6)
0,8115
0,1390
Olor tipico desc
-0,4419
-0,3925
(
6)
(
6)
0,3803
0,4415
pH
-0,1959
0,7259
(
6)
(
6)
0,7099
0,1024
Porcentaje agua perd-0,4948
0,0504
(Dp coc)
(
6)
(
6)
0,3184
0,9244
Porcentaje agua perd 0,6551
0,1773
(CRA)
(
6)
(
6)
0,1579
0,7368
Porcentaje de agua p 0,7740
0,7720
(Hum)
(
6)
(
6)
0,0708
0,0721
Porcentaje Liq perdi-0,0999
-0,4544
(Dp cru)
(
6)
(
6)
0,8506
0,3653
Sabor oxidado
0,2612
0,6499
(
6)
(
6)
0,6171
0,1624
Sabor putrido
0,6717
0,1104
(
6)
(
6)
0,1440
0,8351
Sabor rancio
0,5783
0,7816
(
6)
(
6)
0,2293
0,0663
Sabor tipico
-0,5132
-0,4308
(
6)
(
6)
0,2977
0,3937
Tiempo
0,5114
0,8431
(
6)
(
6)
0,2998
0,0350
----------------------------------------------------------Correlation
(Sample Size)
P-Value
™ Dieta III (Extracto de Romero)
Correlations
Banda 1
Banda 2
---------------------------------------------------------Banda 1
0,6901
(
6)
0,1292
Banda 2
0,6901
(
6)
0,1292
CFU Ent
0,9650
0,7487
(
6)
(
6)
0,0018
0,0868
CFU Prom RAM
-0,2967
0,2048
(
6)
(
6)
0,5680
0,6971
Cohesividad Ciz coc 0,0371
0,6045
(
6)
(
6)
0,9444
0,2037
Cohesividad Ciz crud 0,0832
-0,3522
(
6)
(
6)
0,8754
0,4936
Cohesividad Compresi 0,1262
-0,4703
(
6)
(
6)
0,8117
0,3465
Color
-0,4844
-0,3973
(
6)
(
6)
0,3303
0,4355
Color Roche desc
-0,5723
-0,9463
(
6)
(
6)
0,2353
0,0043
Deshidratacion desc 0,4183
0,8574
(
6)
(
6)
0,4091
0,0291
Dureza N Ciz cocida -0,0768
-0,1441
(
6)
(
6)
0,8850
0,7854
Dureza N Ciz Cruda -0,0790
0,4521
(
6)
(
6)
0,8818
0,3680
Dureza N compresion 0,1826
-0,1456
(
6)
(
6)
0,7292
0,7831
Elasticidad desc
-0,0249
-0,6878
(
6)
(
6)
0,9627
0,1310
Gaping
0,1087
0,7088
(
6)
(
6)
0,8376
0,1148
Goteo desc
0,4335
0,7708
(
6)
(
6)
0,3905
0,0728
Olor putrido desc
0,3832
0,7444
(
6)
(
6)
0,4533
0,0896
Olor rancio
0,2334
0,7858
(
6)
(
6)
0,6563
0,0639
Olor rancio desc
0,1526
0,6357
(
6)
(
6)
0,7729
0,1749
Olor tipico
-0,1243
-0,7829
(
6)
(
6)
0,8145
0,0656
Correlations
Banda 1
Banda 2
---------------------------------------------------------Olor tipico desc
-0,7161
-0,7829
(
6)
(
6)
0,1094
0,0656
pH
0,4758
0,3782
(
6)
(
6)
0,3401
0,4597
Porcentaje agua perd-0,7384
-0,9657
(Dp coc)
(
6)
(
6)
0,0937
0,0017
Porcentaje agua perd 0,5674
0,7130
(CRA)
(
6)
(
6)
0,2403
0,1118
Porcentaje de agua p 0,8924
0,8977
(Hum)
(
6)
(
6)
0,0167
0,0152
Porcentaje Liq perdi-0,4589
0,1638
(Dp cru)
(
6)
(
6)
0,3599
0,7566
Sabor oxidado
0,1902
0,6696
(
6)
(
6)
0,7182
0,1457
Sabor putrido
0,5706
0,8527
(
6)
(
6)
0,2370
0,0309
Sabor rancio
0,2457
0,8063
(
6)
(
6)
0,6388
0,0527
Sabor tipico
-0,2118
-0,8155
(
6)
(
6)
0,6871
0,0479
Tiempo
0,3862
0,9095
(
6)
(
6)
0,4496
0,0119
--------------------------------------------------------Correlation
(Sample Size)
P-Value
ANEXO 37
ANALISIS ESTADISTICO - CORRELACIÓN DEL COMPORTAMIENTO DE AP, GSH
– Px, MMP’s Y PESO DE LOS INDIVIDUOS PARA LAS TRES DIETAS DE
ENGORDA EN ESTUDIO
A.- DIETA I O CONTROL
Correlations
AP
BANDA 1
BANDA 2
GSH PX
PESO
---------------------------------------------------------------------------------AP
-0,4352
-0,2325
0,6950
0,3719
(
6)
(
6)
(
6)
(
6)
0,3884
0,6575
0,1254
0,4678
BANDA 1
BANDA 2
GSH
-0,4352
(
6)
0,3884
-0,2325
(
6)
0,6575
PX
(
0,6950
6)
0,1254
PESO
(
(
0,9195
6)
0,0095
0,9195
6)
0,0095
-0,0473
(
6)
0,9291
-0,0302
(
6)
0,9548
-0,0473
(
6)
0,9291
-0,0964
(
6)
0,8558
-0,0302
(
6)
0,9548
-0,0273
(
6)
0,9591
0,7664
(
6)
0,0755
0,3719
-0,0964
-0,0273
0,7664
6)
(
6)
(
6)
(
6)
0,4678
0,8558
0,9591
0,0755
----------------------------------------------------------------------------------(
Correlation
(Sample Size)
P-Value
B.- DIETA II (EXCESO DE TOCOFEROLES)
Correlations
AP
BANDA 1
BANDA 2
GSH PX
PESO
------------------------------------------------------------------------------AP
-0,3229
-0,1629
0,5300
0,3926
(
6)
(
6)
(
6)
(
6)
0,5325
0,7578
0,2794
0,4413
BANDA 1
-0,3229
(
6)
0,5325
BANDA 2
GSH
(
-0,1629
(
6)
0,7578
PX
(
(
0,5300
6)
0,2794
0,4598
6)
0,3589
0,4598
6)
0,3589
-0,1338
(
6)
0,8006
-0,7920
(
6)
0,0604
0,2000
6)
0,7040
-0,7903
(
6)
0,0613
(
-0,1338
(
6)
0,8006
(
0,2000
6)
0,7040
-0,1550
(
6)
0,7693
PESO
0,3926
-0,7920
-0,7903
-0,1550
6)
(
6)
(
6)
(
6)
0,4413
0,0604
0,0613
0,7693
-----------------------------------------------------------------------------Correlation
(Sample Size)
P-Value
(
C.- DIETA III (EXTRACTO DE ROMERO)
Correlations
AP
BANDA 1
BANDA 2
GSH PX
PESO
-----------------------------------------------------------------------------AP
-0,2431
0,1291
-0,0655
0,3153
(
6)
(
6)
(
6)
(
6)
0,6425
0,8075
0,9018
0,5427
BANDA 1
-0,2431
(
6)
0,6425
BANDA 2
0,1291
6)
0,8075
(
0,6909
6)
0,1285
-0,0655
(
6)
0,9018
(
0,6419
6)
0,1694
(
GSH
PX
(
PESO
(
0,6909
6)
0,1285
0,5890
6)
0,2187
0,6419
(
6)
0,1694
(
0,0077
6)
0,9884
0,5890
(
6)
0,2187
-0,1440
(
6)
0,7854
-0,1422
(
6)
0,7881
0,3153
0,0077
-0,1440
-0,1422
-0,3640
6)
(
6)
(
6)
(
6)
(
6)
0,5427
0,9884
0,7854
0,7881
0,4781
---------------------------------------------------------------------------------(
Correlation
(Sample Size)
P-Value