Re vista ARTÍCULO ORIGINAL Rev Alerg Méx LULTHY"! Mé xic o Análisis in silico de lipocalinas de WLYYVNH[VJHIHSSV]HJHOmTZ[LY` NHSSPUH7VZPISLLMLJ[VLULSLZ[\KPV KLSHZLUMLYTLKHKLZHStYNPJHZ Andrés Sánchez1-3, Ricardo Cardona3, Jorge Sánchez4 9LZ\TLU (5;,*,+,5;,:! SHZSPWVJHSPUHZWHYLJLUL_WSPJHYSHYLHJ[P]PKHKJY\aHKHLU[YLHUPTHSLZJVTVNH[V`WLYYVWLYVWVJVZLOHLZ[\KPHKV HJLYJHKLZ\WHWLSLUSHYLHJ[P]PKHKJY\aHKHJVUV[YVZHUPTHSLZ` Z\LMLJ[VJSxUPJV 6)1,;0=6:! HUHSPaHYWVY[tJUPJHZIPVPUMVYTm[PJHZSHPKLU[PKHKLU[YL SPWVJHSPUHZKLKPMLYLU[LZLZWLJPLZ`L_WSVYHYSH\[PSPKHKKLLZ[HZ[tJUPJHZ LULSLZ[\KPVKLSHZHSLYNPHZ 4(;,90(3@4i;6+6! LZ[\KPVin silicoLULSX\LZLI\ZJHYVU las seJ\LUJPHZKLSPWVJHSPUHZ\[PSPaHUKVLSWYVNYHTH)3(:;3HZZLJ\LUJPHZ KLWYV[LxUHZZLHSPULHYVUJVULSWYVNYHTH*3<:;(36TLNH]LYZP}U KL<UP7YV[3HZZLJ\LUJPHZIHZLKLSVZHSPULHTPLU[VZM\LYVUSHZ SPWVJHSPUHZKLWLYYVZ`NH[VZ3HPKLU[PKHKLU[YLSHZSPWVJHSPUHZZLJVTWHY}JVUSHMYLJ\LUJPHKLZLUZPIPSPaHJP}UHHUPTHSLZLU\UHWVISHJP}U KLWHJPLU[LZHStYNPJVZ 9,:<3;(+6:!SHZZLJ\LUJPHZTVZ[YHYVUPKLU[PKHKLZLU[YL` 3VZ]HSVYLZTmZHS[VZZLLUJVU[YHYVULU[YL*HUM-LSK`-LSK ,X\J3HPKLU[PKHKTmZIHQHM\LJVUSHZSPWVJHSPUHZW\YW\YPUH `WYV[LxUHKL\UP}UHSYL[PUVSKLSNHSSVTLUVYKL6IZLY]HTVZ \UHYLSHJP}ULU[YLLSWH[Y}UKLZLUZPIPSPaHJP}U`LSNYHKVKLPKLU[PKHK LU[YLSHZLZWLJPLZLZ[\KPHKHZ *65*3<:065,:!HWHY[PYKLSVZLZ[\KPVZIPVPUMVYTm[PJVZ`SVZWH[YVULZ KLZLUZPIPSPaHJP}ULUJVU[YHKVZZLWYVWVULX\L-LSK`,X\JZVU WVZPISLZ HSLYNLUVZ TH`VYLZ WHYH NH[V ` JHIHSSV LU SH WVISHJP}U KLS [Y}WPJV`JVTWHY[LUHS[HYLHJ[P]PKHKJY\aHKHJVU*HUM+LIPKVHX\L LZ[VZYLZ\S[HKVZWYV]PLULUKLTVKLSVZWYLKPJ[P]VZKLILUJVUÄYTHYZL JVULZ[\KPVZin vitro e in vivo. 7(3()9(:*3(=,!HSLYNPHH]LZIPVPUMVYTm[PJHJHIHSSVNH[VNHSSPUH PKLU[PKHKSPWVJHSPUHZTHZJV[HZZLUZPIPSPaHJP}U www.nietoeditores.com.mx 1 Universidad deů Magdalena, Departamento de Medicina, Santa Marta, Colombia. 2 Corporación Universitaria Rafael Núñez, Programa de Medicina, Cartagena, Colombia. 3 Grupo de Alergología Clínica y Experimental. IPS hŶŝǀĞƌƐŝƚĂƌŝĂ͕ hŶŝǀĞƌƐŝĚĂĚ ĚĞ ŶƟŽƋƵŝĂ͕ DĞĚĞůůşŶ͕ Colombia. 4 Grupo de Alergología Experimental e InmunogeŶĠƟĐĂ͕ /ŶƐƟƚƵƚŽ ĚĞ /ŶǀĞƐƟŐĂĐŝŽŶĞƐ /ŶŵƵŶŽůſŐŝĐĂƐ͕ Universidad de Cartagena, Cartagena, Colombia. Recibido: 25 de mayo 2015 Aceptado: 27 de noviembre 2015 Correspondencia Dr. Jorge Sánchez [email protected] ƐƚĞĂƌơĐƵůŽĚĞďĞĐŝƚĂƌƐĞĐŽŵŽ Sánchez A, Cardona R, Sánchez J. Análisis in silico de lipocalinas de perro, gato, caballo, vaca, hámster y gallina. Posible efecto en el estudio de las enfermedades alérgicas. Rev Alerg Méx. 2016 ene-mar;63(1):1-10. 1 2016 enero-marzo;63(1) Revista Alergia México 9L](SLYN4t_1HU4HY"! In silicoHUHS`ZPZVM[OLPKLU[P[`VM SPWVJHSPUVMKVNJH[OVYZLJV^OHTZ[LY HUKOLU7VZZPISLYVSLPUHSSLYNPJKPZLHZLZ Andrés Sánchez1-3, Ricardo Cardona3, Jorge Sánchez4 Abstract )(*2.96<5+! 3PWVJHSPUZ ZLLT [V L_WSHPU [OL JYVZZYLHJ[P]P[` IL[^LLU ZVTL WL[Z Z\JO HZ JH[ HUK KVN /V^L]LY P[Z YVSL PU V[OLY HUPTHSZHUKP[ZWVZZPISLJSPUPJHSPTWHJ[PUHSSLYN`KPZLHZLZOH]LILLU ZJHYJLS`Z[\KPLK 6)1,*;0=,! ;V HUHS`aL I` IPVPUMVYTH[PJZ [LJOUPX\LZ [OL PKLU[P[` IL[^LLUSPWVJHSPUVMZVTLHUPTHSZHUK[VL_WSVYL[OLJSPUPJHSPTWHJ[ VUHSSLYNPJKPZLHZLZ 4(;,90(3(5+4,;/6+! (Uin silicoZ[\K`^HZKVUL[VZLHYJOMVY SPWVJHSPUZLX\LUJLZ\ZPUN[OL)3(:;WYVNYHTVM5*)0+H[HIHZL;OL WYV[LPU ZLX\LUJLZ ^LYL HSPNULK ^P[O *3<:;(3 6TLNH <UP7YV[ ]LYZPVUZVM[^HYL;OLIHZLZLX\LUJLZMVYHSPNUTLU[Z^LYLSPWVJHSPUZ KVNZHUKJH[Z;OLKLÄULKWLYJLU[HNLPKLU[P[`^HZJVTWHYLK^P[O[OL MYLX\LUJ`VMZLUZP[PaH[PVU[VHUPTHSZL_WVZLKPUHWVW\SH[PVUVM WH[PLU[Z^P[OZ\ZWLJ[LKHSSLYNPJKPZLHZLZ 9,:<3;:! 0KLU[P[PLZIL[^LLUZLX\LUJLZ^LYL[V;OLOPNOLZ[]HS\LZ^LYLMV\UK^P[O*HUM-LSKHUK-LSK,X\J ;OLSV^LYPKLU[P[`^HZMV\UK^P[OSPWVJHSPUWVYW\YPUHUKYL[PUVS IPUKPUN#>LVIZLY]LKHYLSH[PVUZOPWIL[^LLUZLUZP[PaH[PVUHUK [OLWLYJLU[PKLU[P[`IL[^LLU[OLZWLJPLZZ[\KPLK *65*3<:065:! 3PWVJHSPUZ HZ *HU M -LS KL HUK ,X\ J ZLLT [VWSH`HUPTWVY[HU[YVSLPU[OLJYVZZYLHJ[P]P[`[VJH[OVYZLHUKKVN I\[UV[MVY[OLJVZLUZP[PaH[PVU[VOHTZ[LYJV^VYIPYKZ-LSKLHUK ,X\JJV\SKILHWYL]HSLU[HSSLYNLUMVYOVYZLHUKJH[;OLZLYLZ\S[Z JVTLMYVTWYLKPJ[P]LHUHS`ZPZHUKT\Z[ILJVUÄYTLKI`in vitroHUK in vivoZ[\KPLZ 2,@>69+:! HSSLYN`" IPYKZ" IPVPUMVYTH[PJZ" OVYZL" JH[" OLU" PKLU[P[`" lipocalin; pets; sensitization ANTECEDENTES 3H MYLJ\LUJPH KL H[VWPH ` LUMLYTLKHKLZ HStYNPJHZJVTVHZTHYPUP[PZ`JVUQ\U[P]P[PZLZHS[H ` WHYLJL X\L SH PUJPKLUJPH LZ[m H\TLU[HUKV LZWLJPHSTLU[L LU SVZ WHxZLZ PUK\Z[YPHSPaHKVZ1 2 1 Universidad de Magdalena, Departamento de Medicina, Santa Marta, Colombia. 2 Corporación Universitaria Rafael Núñez, Programa de Medicina, Cartagena, Colombia. 3 Grupo de Alergología Clínica y Experimental. IPS Universitaria, Universidad de ŶƟŽƋƵŝĂ͕DĞĚĞůůşŶ͕ŽůŽŵďŝĂ͘ 4 Grupo de Alergología Experimental e InŵƵŶŽŐĞŶĠƟĐĂ͕/ŶƐƟƚƵƚŽĚĞ/ŶǀĞƐƟŐĂĐŝŽŶĞƐ Inmunológicas, Universidad de Cartagena, Cartagena, Colombia. Correspondence Dr. Jorge Sánchez [email protected] ,U[YLSHZWYPUJPWHSLZM\LU[LZKLHSLYNLUVZLZ[mU SHZ THZJV[HZ" L_PZ[L \U UTLYV JYLJPLU[L KL MHTPSPHZLU3H[PUVHTtYPJHJVUTHZJV[HZLUZ\Z JHZHZ:PSHL_WVZPJP}UWYVSVUNHKHHTHZJV[HZLZ \UMHJ[VYKLYPLZNVKLVWYV[LJ[VYJVU[YHSHHWHYPJP}UKLHSLYNPHZLZ\U[LTHHULUKPZJ\ZP}U" Re vista Sánchez A y col. Análisis in silico de lipocalinas en alergia a mascotas ZPULTIHYNVLU[YLSVZWHJPLU[LZJVUZVZWLJOHKL HSLYNPHZSHZN\xHZTtKPJHZYLJVTPLUKHUYLHSPaHY SHZ TLKPKHZ KL L]P[HJP}U2 WLYV WVY KPMLYLU[LZ TV[P]VZLU[YLLSSVZLTVJPVUHSLZSHTH`VYxHKL SVZWHJPLU[LZ[PLULKPÄJ\S[HKLZWHYHJVUZLN\PY LZ[LVIQL[P]V"HKLTmZSHLMLJ[P]PKHKJSxUPJHKL LZ[HYLJVTLUKHJP}UUVLZJSHYH3 Mé xic o IPVPUMVYTm[PJHZSHPKLU[PKHKLU[YLSHZSPWVJHSPUHZ KLWLYYVNH[VJHIHSSV]HJHOmTZ[LY`NHSSPUH `Z\WVZPISLWHWLSLUSHZHSLYNPHZHHUPTHSLZLU SHWVISHJP}UKLS[Y}WPJV MATERIAL Y MÉTODO Secuencias de lipocalinas <UWYVISLTHHKPJPVUHSWHYHSVNYHYSHZTLKPKHZ KL L]P[HJP}U LZ SH YLHJ[P]PKHK JY\aHKH LU[YL HSN\UHZTHZJV[HZLZWLJPHSTLU[LLU[YLNH[VZ` WLYYVZX\LZVUSHZTHZJV[HZX\LJVUTmZMYLcuencia generan alergias en Europa.,ULZ[\KPVZ WYL]PVZVIZLY]HTVZX\LSHZLUZPIPSPaHJP}UHTHZJV[HZ[HTIPtULZWYL]HSLU[LLUHSN\UHZJP\KHKLZ KL3H[PUVHTtYPJH3HZSPWVJHSPUHZZVUWYV[LxUHZ X\L [YHUZWVY[HU WLX\L|HZ TVStJ\SHZ JVTV LZ[LYVPKLZYL[PUVPKLZ`SxWPKVZ,Z[HMHTPSPHKL WYV[LxUHZLZ[mWYLZLU[LLUSHTH`VYWHY[LKLSVZ THTxMLYVZHSPN\HSX\LLUIHJ[LYPHZULTH[VKVZ L PUZLJ[VZ ` ZVU HSLYNLUVZ PTWVY[HU[LZ JVTV JH\ZHKLSHYLHJ[P]PKHKJY\aHKHLU[YLJHIHSSV NH[V`WLYYV:PULTIHYNVWVJVZLZHILHJLYJH KLSWHWLSKLSHZSPWVJHSPUHZLUSHZLUZPIPSPaHJP}U HV[YHZM\LU[LZJVT\ULZLU3H[PUVHTtYPJH 3VZ HUmSPZPZ IPVPUMVYTm[PJVZ LU[YL SVZ X\L ZL PUJS\`LU SVZ HUmSPZPZ in silico ZPT\SHJP}U WVY JVTW\[HKVYHZZVU\UHOLYYHTPLU[H[PSWHYHLS TVKLSHTPLU[V`SHWYLKPJJP}UKLSHLZ[Y\J[\YHKL SHZWYV[LxUHZ,Z[HZOLYYHTPLU[HZW\LKLUH`\KHY HWYLKLJPYHSLYNLUVZTH`VYLZWV[LUJPHSLZ`W\LKLUL_WSPJHYSHYLHJ[P]PKHKJY\aHKHLU[YLKVZV TmZM\LU[LZKLHSLYNLUVZ:PLU\UHWVISHJP}U SHMYLJ\LUJPHKLZLUZPIPSPaHJP}UH]HYPHZM\LU[LZ LZ JVUVJPKH SVZ HUmSPZPZ KL HSPULHTPLU[V ` SH PKLU[PKHKLU[YLSHZWYV[LxUHZW\LKLUZLYLSWYPTLY WHZV WHYH PKLU[PÄJHY SVZ HSLYNLUVZ WYPUJPWHSLZ X\LL_WSPJHUSVZWH[YVULZKLZLUZPIPSPaHJP}U` JVZLUZPIPSPaHJP}U LU[YL SHZ LZWLJPLZ 7VZ[LYPVYTLU[LLZULJLZHYPVKLTVZ[YHYZPSVZHSLYNLUVZ PKLU[PÄJHKVZ JVTV WYVIHISLZ ZVU YLHSTLU[L HSLYNLUVZTH`VYLZTLKPHU[LLZ[\KPVZin vivo. En LZ[LLZ[\KPVL_WSVYHTVZWVYTLKPVKL[tJUPJHZ ,Z[\KPVin silicoLULSX\LZLI\ZJHYVUSPWVJHSPUHZ\[PSPaHUKVLSWYVNYHTH)3(:;KLSHIHZL 5*)0JVUSHZZLJ\LUJPHZ]HSPKHKHZOHZ[HLS KLUV]PLTIYLKL:}SVZLPUJS\`LYVUSHZ WYV[LxUHZ HSLYNtUPJHZ JVU \UH PKLU[PÄJHJP}U JVTWSL[HKLSHZLJ\LUJPHKL(95TLUZHQLYVKL WLYYVNH[VJHIHSSVOmTZ[LY`]HJH(KLTmZZL PUJS\`LYVUKVZSPWVJHSPUHZKLNHSSPUHUVPKLU[PÄJHKHZJVTVHSLYNtUPJHZW\YW\YPUH`WYV[LxUHKL \UP}UHYL[PUVS!9)7WHYH[LULYZLJ\LUJPHZJVTWHYH[P]HZKLLZ[LNY\WV[H_VU}TPJV3HZLU[YHKHZ KLZLJ\LUJPHZWHYJPHSLZVJVY[LZHS[LYUH[P]VZZL L_JS\`LYVU(SN\UHZSPWVJHSPUHZWV[LUJPHSTLU[L HSLYNtUPJHZJVTV,X\JUVZLPUJS\`LYVULU LZ[LLZ[\KPVKLIPKVHX\LSHZLJ\LUJPHYLWVY[HKH LUSHIHZL5*)0LZPUJVTWSL[H 3HZZLJ\LUJPHZWYV[LPJHZZLHSPULHYVU\ZHUKVLS WYVNYHTH *9<:;(3 6TLNH ]LYZP}U KL <UP7YV[3HZZLJ\LUJPHZIHZLWHYHSVZHSPULHTPLU[VZM\LYVUSHZSPWVJHSPUHZKLWLYYV`NH[VKLIPKV HX\LZVUSHZTHZJV[HZTmZJVT\ULZLUSHZJHZHZ Análisis filogenéticos 3VZHUmSPZPZÄSVNLUt[PJVZIHZHKVZLUSHZZLJ\LUJPHZKL(95ZLJ\LUJPHZKLU\JSL}[PKVZZL YLHSPaHYVUTLKPHU[LLSTt[VKVKLYLJVUZ[Y\JJP}U KL 5LPNOIVY1VPUPUN JVU LS WYVNYHTH 4,.( (molecular evolutionary genetics analysis) ]LYZP}U \ZHUKVLSTt[VKVbootstrapJVTVWY\LIHKL ÄSVNLUPHJVUYLWSPJHJPVULZ7HYHKL[LYTPUHY SHKPZ[HUJPHÄSVNLUt[PJH\[PSPaHTVZLSTt[VKVKL SH Tm_PTH WYVIHIPSPKHK JVTW\LZ[H [LUPLUKV LU J\LU[H LS UTLYV KL Z\Z[P[\JPVULZ KL IHZL 3 2016 enero-marzo;63(1) Revista Alergia México WVYJHKHZP[PV;VKHZSHZWVZPJPVULZJVULZWHJPVZ ]HJxVZ ZL LSPTPUHYVU KLSLJPVULZ JVTWSL[HZ 7HYH [VKVZ SVZ JY\JLZ SHZ WVZPJPVULZ KL JVK}U PUJS\PKHZWHYHSVZHUmSPZPZM\LYVUWYPTLYVZLN\U KV[LYJLYVZLJ\LUJPHZUVJVKPÄJHU[LZ+LIPKV HSUTLYVKLZLJ\LUJPHZKLSPWVJHSPUHZ\[PSPaHKHZ UVYLHSPaHTVZZ\IHUmSPZPZÄSVNLUt[PJVZ 3L[PL0UT\UV[LR4HKYPK,ZWH|H`SHZYLJVTLUKHJPVULZPU[LYUHJPVUHSLZWHYHZ\SLJ[\YHOHI}U TTTH`VYX\LLSJVU[YVSULNH[P]V ;VKVZ SVZ WHJPLU[LZ Z\ZWLUKPLYVU SH HKTPUPZ[YHJP}U KLHU[POPZ[HTxUPJVZ\V[YVZMmYTHJVZLS[PLTWV YLX\LYPKVHU[LZKLSHWY\LIH Consideraciones éticas Selección de la población <ZHTVZ\UHJVOVY[LKLWHJPLU[LZWYL]PHTLU[L YLWVY[HKH ` HNYLNHTVZ WHJPLU[LZ U\L]VZ YLJS\[HKVZK\YHU[LLSWLYPVKVKLQ\UPVKLH THYaVKL9LJVSLJ[HTVZWHJPLU[LZJVU KPHNU}Z[PJVKLHZTHYPUP[PZVKLYTH[P[PZH[}WPJH X\LHZPZ[PLYVUHSZLY]PJPVKL(SLYNVSVNxH*SxUPJH KLSH<UP]LYZPKHKKL(U[PVX\PH4LKLSSxU*VSVTIPH`YLX\PYPLYVU\UHWY\LIHJ\[mULHJVU HSLYNLUVZWHYHPKLU[PÄJHYSVZWVZPISLZHSLYNLUVZ HTIPLU[HSLZ+\YHU[LSHJP[HWHYHSHWY\LIHZL PU[LYYVN}HSVZWHJPLU[LZHJLYJHKLSVZKL[VUHU[LZZVZWLJOVZVZ`SHL_PZ[LUJPHKLTHZJV[HZLU JHZHVSHL_WVZPJP}UPUKPYLJ[HHHUPTHSLZ3\LNV KLSHWY\LIHJ\[mULHSVZWHJPLU[LZYLJPIPLYVU PUMVYTHJP}U KL THULYH ]LYIHS ` WVY LZJYP[V HJLYJHKLSHZTLKPKHZKLJVU[YVSHTIPLU[HSWHYH SHZM\LU[LZHSHZX\LLZ[\]PLYVUZLUZPIPSPaHKVZ *VTV\UJYP[LYPVKLPUJS\ZP}UH[VKVZSVZWHJPLU[LZZLSLZL]HS\}SHZLUZPIPSPaHJP}UHBlomia tropicalis, Dermatophagoides pteronyssinus, Dermatophagoides farinae LWP[LSPV KL NH[V ` WLYYV. 0UJS\PTVZ L_[YHJ[VZ HKPJPVUHSLZ KL animales en los pacientes con contacto o con ZVZWLJOH KL ZxU[VTHZ WVY V[YVZ HUPTHSLZ WVY LQLTWSV JHIHSSV OmTZ[LY WmQHYVZ NHSSPUHZ L[J,SKPHNU}Z[PJVZLLZ[HISLJP}KLHJ\LYKVJVU SHZN\xHZ.05(KLHZTH^^^NPUHZ[OTHVYN (90(KLYPUP[PZ6LS*VUZLUZV3H[PUVHTLYPJHUVKL *VUQ\U[P]P[PZ`SVZJYP[LYPVZKL/HUPÄU`9HQRH KLKLYTH[P[PZH[}WPJH 7HYHSHWY\LIHJ\[mULHPU[YHLWPKtYTPJH\ZHTVZ SVZL_[YHJ[VZLZ[HUKHYPaHKVZKLSVZSHIVYH[VYPVZ 4 7HYHSHYL]PZP}UKLSHZOPZ[VYPHZJSxUPJHZ`SVZ KH[VZKLSVZWHJPLU[LZZLVI[\]VSHHWYVIHJP}U KLSJVTP[tKLt[PJHKLSHPUZ[P[\JP}UOVZWP[HSHYPH 3HZ ZLJ\LUJPHZ ZL [VTHYVU KL SH IHZL KLKH[VZKL5*)0WVYSVX\LZVUKLHJJLZV abierto. Análisis estadísticos 3VZHUmSPZPZZLYLHSPaHYVUJVULSWYVNYHTH0)4 :7::]LYZP}UWHYH>PUKV^Z3VZYLZ\S[HKVZ ZLL_WYLZHYVUJVTVWVYJLU[HQLZKLMYLJ\LUJPH `LUUTLYVZHIZVS\[VZ:L\ZHYVUHUmSPZPZKL JVYYLSHJP}UWY\LIHKLr2VYLNYLZPVULZSVNxZ[PJHZ T\S[P]HYPHISLZWHYHTLKPYSHZKPMLYLUJPHZLU[YL los grupos. RESULTADOS Identidad de las lipocalinas 3HZ SPWVJHSPUHZ ZLSLJJPVUHKHZ ZL T\LZ[YHU LU LS *\HKYV :L YLHSPaHYVU HSPULHTPLU[VZ LU[YL SHZ SPWVJHSPUHZ PUJS\PKHZ 3HZ ZLJ\LUJPHZ IHZLZ\[PSPaHKHZM\LYVUSHZSPWVJHSPUHZKLWLYYV ` NH[V *\HKYV 3HZ ZLJ\LUJPHZ TVZ[YHYVU \UHNYHU]HYPHIPSPKHKLU[YLSHZPKLU[PKHKLZ H3HTH`VYPKLU[PKHKZLLUJVU[Y}LU[YL SVZHSPULHTPLU[VZKL*HUM-LSKJVU]HSVYLZ KL`-LSK,X\JJVUZLN\PKVWVY *HUM-LSKJVU`*HUM,X\JJVU -PN\YH3HTLUVYPKLU[PKHKZLLUJVU[Y} LU[YL SVZ HSPULHTPLU[VZ WHYH WVYW\YPUH ` 9)7 JVUSHZV[YHZLZWLJPLZX\LM\LTLUVYKL *\HKYV` Re vista Mé xic o Sánchez A y col. Análisis in silico de lipocalinas en alergia a mascotas *\HKYV. 3PWVJHSPUHZZLSLJJPVUHKHZWHYHSVZHSPULHTPLU[VZ 3PWVJHSPUH Código )HZLKLKH[VZ ZLJ\LUJPH(95T Entrada (Swissprot) 5TLYVKL HTPUVmJPKVZ Perro (Canis familiaris) (- (- .< +7)/ /, /). .H[V(Felis domesticus o catus) (33F-,3*( (@ 8=-/ ,+AF-,3*( .< ,+A )V]PUL(Bos taurus) (33F)6=05 3 8 Caballo (Equus caballus) (33F/69:, < 8 .HSSPUHNHSSV(Gallus gallus o domesticus) 7<97F*/0*2 4 7 9,;F*/0*2 ? 7 /mTZ[LY(Phodopus sungorus) :A@+F7/6:< 2- :A@+ *HUM *HUM *HUM *HUM (33F*(5-( (33F*(5-( +7)/F*(5-( /).F*(5-( -LSK -LSK )VZK ,X\J 7\YW\YPUH 9)7 7OVZR+H 3PWVJHSPUHZZLSLJJPVUHKHZKLKPMLYLU[LZHUPTHSLZWHYHHUmSPZPZIPVPUMVYTm[PJVZ 9)7!WYV[LxUHKL\UP}UHSYL[PUVSretinol binding proteins *\HKYV. 0KLU[PKHKLU[YLSHZSPWVJHSPUHZKLWLYYV`NH[V (SPULHTPLU[V 0KLU[PKHK 5TKL 5TKL HTPUVmJPKVZ HTPUVmJPKVZ idénticos ZPTPSHYLZ Perro-gato *HUM-LSK *HUM-LSK *HUM-LSK *HUM-LSK *HUM-LSK *HUM-LSK *HUM-LSK *HUM-LSK 23 61 23 110 130 61 66 62 *VUSHSPWVJHSPUHKLOmTZ[LY7OVZR+HSH PKLU[PKHKJVUV[YHZLZWLJPLZ[HTIPtUM\LIHQH! *HUM7OVZR+H!-LSK7OVZ R+H!3HSPWVJHSPUHKLSH]HJH)VZKTVZ[Y}]HSVYLZLU[YL`SHTH`VYPKLU[PKHK ZLLUJVU[Y}JVU*HUMJVU\U]HSVYKL *\HKYVZ` Análisis filogenéticos ,SmYIVS}W[PTVJVU\UHSVUNP[\KKLYHTPÄJHJP}U PN\HSH ZLT\LZ[YHLUSH-PN\YH ,SWVYJLU[HQLKLmYIVSLZYLWSPJHKVZYLWL[PJPVULZZLT\LZ[YHLUSH-PN\YH,SmYIVSZL T\LZ[YHHLZJHSHSHSVUNP[\KKLSHYHTPÄJHJP}U YLWYLZLU[HSHKPZ[HUJPHL]VS\[P]HLUSHJHU[PKHK KLJHTIPVZHJ\T\SHKVZ"ZPULTIHYNVZLKLIL [LULYLUJ\LU[HX\LLSTt[VKV5LPNOIVY1VPUPUN Z}SVOHJLHNY\WHJPVULZKL¸TPLTIYVZZPTPSHYLZ¹ ZPU [VTHY LU J\LU[H LS YLSVQ TVSLJ\SHY WVY SV X\L SH KPZ[HUJPH L]VS\[P]H LZ Z}SV HWYV_PTHKH KL HJ\LYKV JVU SH PKLU[PKHK KL SHZ WYV[LxUHZ LZ[\KPHZ/\IVWVZPJPVULZLUSHIHZLÄUHS Características de la población y sensibilización a animales +LSVZWHJPLU[LZ LYHUT\QLYLZ*\HKYVZ `3HLKHKTLKPHM\LKLH|VZ3HTH`VYxH KLSVZWHJPLU[LZ[LUxHULUMLYTLKHKLZYLZWPYH[VYPHZ 5 2016 enero-marzo;63(1) Revista Alergia México -PN\YH,Z[Y\J[\YHKLHSPULHTPLU[VLU[YL*HUM-LSK`,X\J3VZHTPUVmJPKVZPKLU[PÄJHKVZLZ[mUPUKPJHKVZJVU¸*¹SVZHTPUVmJPKVZZPTPSHYLZLZ[mUPUKPJHKVZJVU¸!¹`YLZHS[HKVZLUNYPZVZJ\YV `WHJPLU[LZ[LUxHUZxU[VTHZYLZWPYH[VYPVZ`J\[mULVZ"WHJPLU[LZ[LUxHU H[VWPHWHJPLU[LZM\LYVUWVZP[P]VZH\UV V]HYPVZHUPTHSLZ,SWLYYVU$`LSNH[V U$ M\LYVUSHZM\LU[LZJH\ZHU[LZJVUTmZ MYLJ\LUJPHKLZLUZPIPSPaHJP}ULU[YLTHZJV[HZ" WHJPLU[LZWVZP[P]VZHNH[VSVM\LYVU[HTIPtUHWLYYV :LPZWHJPLU[LZM\LYVUWVZP[P]VZHJHIHSSV`[VKVZ LSSVZSVM\LYVUHNH[V`WLYYV*\H[YVKLLSSVZ[LUxHU \UHHS[HL_WVZPJP}UHJHIHSSV"WHJPLU[LZ M\LYVUWVZP[P]VZHWmQHYVZKLLSSVZM\LYVUWVZP[P]VZHNH[VVWLYYV"WHJPLU[LZWVZP[P]VZHWLYYV YLZ\S[HYVUZLUZPIPSPaHKVZHmJHYVZ`[VKVZSVZ WHJPLU[LZWVZP[P]VZHNH[VSVM\LYVUHSVZmJHYVZ" WHJPLU[LZLZ[\]PLYVUL_W\LZ[VZHHSNU HUPTHS!HWLYYVHNH[V HWmQHYVZ`HV[YVZHUPTHSLZJVTV JHIHSSVOmTZ[LY`JVULQV5VW\KPTVZL]HS\HYZP L_PZ[xH\UHYLSHJP}ULU[YLSHL_WVZPJP}UHTHZJV[HZ `SVZZxU[VTHZKLIPKVHX\LSHWVISHJP}ULZ[HIH JVUMVYTHKH [V[HSTLU[L WVY WHJPLU[LZ HStYNPJVZ ` UV OHIxH JVU[YVSLZ 3H UPJH HZVJPHJP}U LU[YL L_WVZPJP}U`ZLUZPIPSPaHJP}UM\LHWmQHYVZW# TLKPH!0* ! 6 Relación entre identidad de lipocalinas y patrón de sensibilización 6IZLY]HTVZ X\L LS WVYJLU[HQL KL PKLU[PKHK KL HSN\UHZSPWVJHSPUHZLZ[mLZ[YLJOHTLU[LYLSHJPVUHKV JVULSWH[Y}UKLZLUZPIPSPaHJP}U3HTH`VYPKLU[PKHKZLLUJVU[Y}WHYH*HUM-LSK`,X\J JVUMYLJ\LUJPHHS[HKLJVZLUZPIPSPaHJP}UHWLYYV NH[V`JHIHSSVWTLUVYKL3HZSPWVJHSPUHZ KLNHSSPUHOmTZ[LY`]HJH[LUxHUWVJHPKLU[PKHK `WVJHMYLJ\LUJPHKLJVZLUZPIPSPaHJP}UJVUNH[V` WLYYV+LIPKVHX\LLZ[HHZVJPHJP}UWVKYxHLZ[HY YLSHJPVUHKHJVULSNYHKVKLL_WVZPJP}UYLHSPaHTVZ \U Z\IHUmSPZPZ JVUZPKLYHUKV SH L_WVZPJP}U HUPTHSJVTV\UHJV]HYPHISL`UVVIZLY]HTVZ\UH YLSHJP}U ZPNUPÄJH[P]H LU[YL SH L_WVZPJP}U KPYLJ[H HJHIHSSVOmTZ[LY]HJHNHSSPUHNH[VVWLYYV` sensibilización. DISCUSIÓN 3VZ WH[YVULZ KL ZLUZPIPSPaHJP}U W\LKLU ]HYPHY KL HJ\LYKV JVU SHZ JVUKPJPVULZ NLVNYmÄJHZ ` ZVJPVJ\S[\YHSLZ KL \UH YLNP}U10-12 +L HJ\LYKV Re vista Mé xic o Sánchez A y col. Análisis in silico de lipocalinas en alergia a mascotas *\HKYV.0KLU[PKHKLU[YLSPWVJHSPUHZ\ZHUKVSHZZLJ\LUJPHZ KLSWLYYVJVTVIHZL (SPULHTPLU[V *HUM)VZK *HUM)VZK *HUM)VZK *HUM)VZK *HUM,X\J *HUM,X\J *HUM,X\J *HUM,X\J *HUM7\YW\YPU *HUM7<9 *HUM7\YW\YPU *HUM7<9 *HUM7\YW\YPU *HUM7<9 *HUM7\YW\YPU *HUM7<9 *HUM7OVZR+H *HUM7OVZR+H *HUM7OVZR+H *HUM7OVZR+H 0KLU[PKHK 5TKL 5TKL HTPUVmJPKVZHTPUVmJPKVZ idénticos ZPTPSHYLZ Caballo-bovino 31 Perro-caballo 26 111 Perro-gallina 13 13 13 36 33 7LYYVOmTZ[LY 31 13 21 63 63 63 66 63 60 JVULS4PUPZ[LYPVKL:HS\KKL*VSVTIPH^^^ TPUWYV[LJJPVUZVJPHSNV]JV`SH:VJPLKHK7YV[LJ[VYHKL(UPTHSLZHSYLKLKVYKL\UHKLJHKH [YLZJHZHZLULS=HSSLKL(I\YYm[PLULTHZJV[HZ WLYYVZ`NH[VZ`[PLULHSTLUVZKVZTHZJV[HZKLLZWLJPLZKPMLYLU[LZ,Z[VZ KH[VZZ\NPLYLUX\LOH`\UHHS[HJVUJLU[YHJP}U KLWHY[xJ\SHZKLYP]HKHZKLHUPTHSLZLULSHPYL KL4LKLSSxU:PULTIHYNVUVLUJVU[YHTVZ\UH YLSHJP}UKPYLJ[HLU[YLSHMYLJ\LUJPHKLL_WVZPJP}U H NH[VZ V WLYYVZ ` ZLUZPIPSPaHJP}U ,Z[V W\LKL L_WSPJHYZLWVYSHHS[HMYLJ\LUJPHKLL_WVZPJP}U PUKPYLJ[HLUSHLZJ\LSHLS[YHIHQVVLS]LJPUKHYPV X\LLZKPMxJPSKLJ\HU[PÄJHY`JVUZPKLYHUKVSVZ KH[VZ LWPKLTPVS}NPJVZ JVTLU[HKVZ tZ[H KLIL ser alta en esta región. *\HKYV.0KLU[PKHKLU[YLSPWVJHSPUHZ\ZHUKVHNH[VJVTV secuencia base (SPULHTPLU[V 0KLU[PKHK 5TKL 5TKL HTPUVmJPKVZHTPUVmJPKVZ idénticos ZPTPSHYLZ .H[VIV]PUV .H[VJHIHSSV -LSK,X\J 130 -LSK,X\J .H[VNHSSPUH -LSK7\YW\YPUH 16 -LSK7<9 -LSK7\YW\YPUH 36 -LSK7<9 .H[VOmTZ[LY -LSK7OVZR+H 36 -LSK7OVZR+H -LSK)VZK -LSK)VZK 30 60 60 6[YV MHJ[VY PTWVY[HU[L X\L W\LKL YLWLYJ\[PY en la alta sensibilización a estos animales es SHYLHJ[P]PKHKJY\aHKHHSHMHTPSPHKLWYV[LxUHZ SPWVJHSPUHZ,U[YLSHZLZWLJPLZKLWLYYVNH[V` caballo13 LZ[HZP[\HJP}UMH]VYLJLSHJVZLUZPIPSPaHJP}ULU[YLWHJPLU[LZX\L[PLULUWVJHL_WVZPJP}U HSVZHUPTHSLZ,UU\LZ[YHWVISHJP}ULZ[VWVKYxH L_WSPJHY HS TLUVZ LU WHY[L SH HS[H JVZLUZPIPSPaHJP}U H JHIHSSV NH[V ` WLYYV *VU IHZL LU SVZ HUmSPZPZ IPVPUMVYTm[PJVZ Z\NLYPTVZ X\L SH JVZLUZPIPSPaHJP}UWVKYxHZLYWVYSHHS[HPKLU[PKHK LU[YL*HUM-LSK`,X\J+LHJ\LYKVJVU LSWH[Y}UKLZLUZPIPSPaHJP}U[VKVZSVZWHJPLU[LZ ZLUZPIPSPaHKVZHJHIHSSVSVLZ[\]PLYVU[HTIPtUH WLYYVWVYSVX\L,X\JWVKYxHZLYLSWYPUJPWHS HSLYNLUVKLJHIHSSV7VYSHYLHJ[P]PKHKJY\aHKH *HUMW\LKLZLY\UPTWVY[HU[LHSLYNLUVWHYH WLYYVWLYVUVLSUPJVWVYX\LSHTH`VYxHKLSVZ WHJPLU[LZZLUZPIPSPaHKVZHWLYYVUVSVLZ[\]PLYVU HNH[VVJHIHSSV-LSKWVKYxHZLY\UHSLYNLUV importante para gato en nuestra población si se [VTHLUJ\LU[HSHHS[HPKLU[PKHKX\L[PLULJVU *HUM`SHHS[HMYLJ\LUJPHKLJVZLUZPIPSPaHJP}UH WLYYVLU[YLSVZWHJPLU[LZZLUZPIPSPaHKVZHNH[V"ZPU LTIHYNVLZ[HZWYLKPJJPVULZKLILUJVUÄYTHYZL 7 2016 enero-marzo;63(1) Revista Alergia México ZLS K -L SPZ JH [\ PU V[L Y NW KPU IPU VS U L[P ,X\\ZJHIHSS\Z,X\J ZY SS\ NH . 0.2 S\Z 10 W\Y 0 W\Y M HU 0 NHS Z* PHYP 10 . TPS ZMH PU UP *H Z-LSK -LSPZJH[\ 60 P H PSP U *H YPZ 7O VK T *HUM VW \Z Z\ UN VY [H )VZ \Z 7 OV Z 2+ K )VZ \Y\Z YPZ* HUM PZMHT PSPH *HU U *H TPSPHYPZ M H ZM *HUPZMH 100 -PN\YHÍYIVSÄSVNLUt[PJV ,SmYIVS}W[PTVJVUSHZ\THKLSHSVUNP[\KKLSHYHTHLZPN\HSH WVY Tt[VKVZ in vivo o in vitro 5\LZ[YVZ YLZ\S[HKVZZVUZPTPSHYLZHSVZLUJVU[YHKVZWVYV[YVZ H\[VYLZSVX\LZ\NPLYL\UHHS[HWYVIHIPSPKHKKL YLHJ[P]PKHKJY\aHKHKLTVZ[YHKHWVYHUmSPZPZKL PUOPIPJP}U ` JVTWL[PJP}U \[PSPaHUKV SH [tJUPJH KL,30:(LU[YL-LSK*HUM`,X\J+L 8 J\HSX\PLYTHULYHSHMYLJ\LUJPHKLZLUZPIPSPaHJP}UHNH[VWLYYV`JHIHSSVLZKPMLYLU[LLU[YLSHZ WVISHJPVULZWVYSVX\LSVZMHJ[VYLZKLSHTIPLU[L `SHZJHYHJ[LYxZ[PJHZNLUt[PJHZKLJHKHWVISHJP}U PUÅ\`LUKLTHULYHPTWVY[HU[LLUSHWYL]HSLUJPH KLSHZLUZPIPSPaHJP}UHKPMLYLU[LZM\LU[LZ Re vista Sánchez A y col. Análisis in silico de lipocalinas en alergia a mascotas *\HKYV *HYHJ[LYxZ[PJHZNLULYHSLZKLSHWVISHJP}UU$ +H[VZNLULYHSLZ 5T :L_V 4\QLY /VTIYL -LUV[PWVZ (ZTH 9PUP[PZ *VUQ\U[P]P[PZ Eccema ,KHKH|VZ 4LKPHSxTP[LZ!H *\HKYV *HYHJ[LYxZ[PJHZKLSHWVISHJP}UWVYZLUZPIPSPaHJP}U U$ +H[VZNLULYHSLZ ([VWPH 7LYYV .H[V 7mQHYVZ *HUHYPVZWLYPJVZWHSVTHZ`NHSSPUHZ 6[YVZHUPTHSLZ *HIHSSVJVULQVOmTZ[LYJLYKV 5T U$U$ *VTWHYHKV JVU V[YHZ LZWLJPLZ ZL ZHIL WVJV HJLYJHKLSHMHTPSPHKLSPWVJHSPUHZ`Z\ZWYVWPLKHKLZHSLYNtUPJHZLUSHNHSSPUH]HJH`OmTZ[LY16 6IZLY]HTVZ \UH IHQH JVZLUZPIPSPaHJP}U H OmTZ[LYV]HJHJVUNH[VVWLYYV,Z[HIHQHJVZLUZPIPSPaHJP}UJVUJ\LYKHJVUSHIHQHPKLU[PKHK LU[YLSHZSPWVJHSPUHZKLLZ[HZLZWLJPLZ`Z\TH`VY KPZ[HUJPH ÄSVNLUt[PJH ,_PZ[LU WVJVZ LZ[\KPVZ HJLYJHKLSHZLUZPIPSPaHJP}UHSVZWmQHYVZ 6IZLY]HTVZX\LSHMYLJ\LUJPHKLZLUZPIPSPaHJP}U HWmQHYVZJVTVNY\WVM\LTH`VYKLWLYV SHZLUZPIPSPaHJP}UHNHSSPUHWHSVTHWLYPX\P[VV JHUHYPVZLWHYHKHTLU[LM\LTLUVYKL Esta IHQHMYLJ\LUJPHKLZLUZPIPSPaHJP}UJVTWHYHKH JVUSHKLNH[VVWLYYVW\LKLKLILYZLHX\LWVJHZWLYZVUHZLUU\LZ[YHWVISHJP}U[PLULUWmQHYVZ JVTVTHZJV[HWLYV[HTIPtUW\LKLKLILYZLHSH WVJHPKLU[PKHKJVUSHZSPWVJHSPUHZKLV[YHZLZWLJPLZJVTVNH[VVWLYYV3HL_WVZPJP}UKPYLJ[H WHYLJL[LULYTH`VYPTWVY[HUJPHLUSHZLUZPIPSP- Mé xic o aHJP}U H WmQHYVZ X\L SH YLHJ[P]PKHK JY\aHKH H V[YHZLZWLJPLZ:PULTIHYNVZ}SV[VTHTVZSHZ SPWVJHSPUHZKLNHSSPUHKLIPKVHX\LOHZ[HU\LZ[YVJVUVJPTPLU[VZVUSHZUPJHZYLWVY[HKHZHS TVTLU[VWVYSVX\LUVWVKLTVZHZLN\YHYX\L SHIHQHPKLU[PKHKVIZLY]HKHJVUNH[V`WLYYVZL L_[PLUKHHV[YHZLZWLJPLZKLWmQHYVZ 5\LZ[YVLZ[\KPV[PLULHSN\UHZMVY[HSLaHZ`SPTP[HJPVULZ+LIPKVHX\LLZ\ULZ[\KPVin silico, las HZVJPHJPVULZLUJVU[YHKHZLU[YLSVZWH[YVULZKL JVZLUZPIPSPaHJP}U`SHPKLU[PKHKKLSHZSPWVJHSPUHZ UVZWLYTP[LL_WSVYHYSVZHSLYNLUVZX\LW\LKLU [LULY TH`VY WVZPIPSPKHK KL ZLY JSxUPJHTLU[L YLSL]HU[LZ LU U\LZ[YH WVISHJP}U Z\ LMLJ[V LU SHYLHJ[P]PKHKJY\aHKHLPKLU[PÄJHYSHZWVZPISLZ M\LU[LZ WYPUJPWHSLZ KL ZLUZPIPSPaHJP}U :PU LTIHYNVLZ[VZYLZ\S[HKVZHSZLYWYLKPJ[P]VZKLILU JVUÄYTHYZLJVULZ[\KPVZin vitro WHYHYH[PÄJHYX\L SVZHSLYNLUVZPKLU[PÄJHKVZZVUSVZJH\ZHU[LZKL SHYLHJ[P]PKHKJY\aHKH`SHZMYLJ\LUJPHZKLZLUZPIPSPaHJP}ULUJVU[YHKHZ"ZPULTIHYNVtZ[LZLYxHLS WYPTLYWHZVWHYH\UHIZX\LKHin vitroKPYPNPKH CONCLUSIÓN +LHJ\LYKVJVUSVZHUmSPZPZIPVPUMVYTm[PJVZ` SVZWH[YVULZKLZLUZPIPSPaHJP}ULU\UHWVISHJP}U KLS[Y}WPJVWYVWVULTVZX\LHSN\UHZSPWVJHSPUHZ W\LKLU[LULY\UPTWVY[HU[LWHWLSLUSHJVZLUZPIPSPaHJP}UHNH[VWLYYV`JHIHSSVWLYVUVWHYH OmTZ[LY]HJHVWmQHYVZ-LSK`,X\JWVKYxHU ZLYHSLYNLUVZTH`VYLZWHYHJHIHSSV`NH[V Agradecimientos ,S.Y\WVKL(SLYNVSVNxH*SxUPJH`,_WLYPTLU[HS .(*,KLSH<UP]LYZPKHKKL(U[PVX\PH4LKLSSxU*VSVTIPHÄUHUJP}LZ[LLZ[\KPV REFERENCIAS 1. Dennis RJ, Caraballo L, García E, Rojas MX, et al. Prevalence ŽĨĂƐƚŚŵĂĂŶĚŽƚŚĞƌĂůůĞƌŐŝĐĐŽŶĚŝƟŽŶƐŝŶŽůŽŵďŝĂϮϬϬϵͲ ϮϬϭϬ͗ĂĐƌŽƐƐͲƐĞĐƟŽŶĂůƐƚƵĚLJ͘DWƵůŵDĞĚϮϬϭϮ͖ϭϮ͗ϭϳ͘ 9 Revista Alergia México 2. Macías Weinmann A, Escamilla Weinmann C, Pazos Salazar E'͕ sĂůĚĠƐ ƵƌŶĞƐ ͕ 'ŽŶnjĄůĞnj şĂnj ^E͘ ^ĞŶƐŝƟǀŝƚLJ ƚŽ animals’ allergens in people working with animals]. Rev ůĞƌŐDĞdžϮϬϭϬ͖ϱϳ͗ϭϴϱͲϭϴϵ͘ 12. Liccardi G, Salzillo A, Piccolo A, D’Amato G. Skin prick test to horse should be included in the standard panel for the ĚŝĂŐŶŽƐŝƐ ŽĨ ƌĞƐƉŝƌĂƚŽƌLJ ĂůůĞƌŐLJ͘ : /ŶǀĞƐƟŐ ůůĞƌŐŽů ůŝŶ /ŵŵƵŶŽůϮϬϭϬ͖ϮϬ͗ϵϯͲϵϰ͘ ϯ͘ ĚĞ:ŽŶŐ͕ŝŬŬĞƐĐŚĞŝ>͕ƌĂŶĚW>͘^ĞŶƐŝƟnjĂƟŽŶƉĂƩĞƌŶƐ to food and inhalant allergens in childhood: a comparison of non-sensitized, monosensitized, and polysensitized children. Pediatr Allergy Immunol 2011;22:166-171. ϭϯ͘ ŽƌƌĞƐ DW͕ ďŝƐĂǁĂ D͕ ŝŐĞŶŵĂŶŶ W͘ hƐĞ ŽĨ ĂůůĞƌŐĞŶ components begins a new era in pediatric allergology. Pediatr Allergy Immunol 2011;22:454-461. ϰ͘ ƵƌďĂĐŚ'͕,ĞŝŶnjĞƌůŝŶŐ>͕ĚĞŶŚĂƌƚĞƌ'͕ĂĐŚĞƌƚ͕ĞƚĂů͘ GA(2)LEN skin test study II: clinical relevance of inhalant ĂůůĞƌŐĞŶ ƐĞŶƐŝƟnjĂƟŽŶƐ ŝŶ ƵƌŽƉĞ͘ ůůĞƌŐLJ ϮϬϬϵ͖ϲϰ͗ϭϱϬϳͲ 1515. ϭϰ͘ ,ŝůŐĞƌ͕<ƵĞŚŶ͕,ĞŶƚŐĞƐ&͘ŶŝŵĂůůŝƉŽĐĂůŝŶĂůůĞƌŐĞŶƐ͘ Curr Allergy Asthma Rep 2012;12:438-447. ϭϱ͘ EŝůƐƐŽŶK͕ŝŶŶŵLJƌ:͕ŽůƚŽǁƐŬĂ͕^ĂĂƌŶĞd͕ĞƚĂů͘ŚĂƌĂĐƚĞƌŝnjĂƟŽŶŽĨƚŚĞĚŽŐůŝƉŽĐĂůŝŶĂůůĞƌŐĞŶĂŶĨϲ͗ƚŚĞƌŽůĞŝŶ ĐƌŽƐƐͲƌĞĂĐƟǀŝƚLJǁŝƚŚĐĂƚĂŶĚŚŽƌƐĞ͘ůůĞƌŐLJϮϬϭϮ͖ϲϳ͗ϳϱϭͲϳϱϳ͘ 5. Sánchez J, Diez S, Cardona R. Frecuencia de sensibilización a ĂŶŝŵĂůĞƐĞŶƵŶĄƌĞĂƚƌŽƉŝĐĂů͘ZĞǀůĞƌŐDĞdžϮϬϭϰ͖ϲϭ͗ϴϭͲϴϵ͘ ϲ͘ ƌŽnjĞŬ:>͕ŽƵƐƋƵĞƚ:͕ĂĞŶĂͲĂŐŶĂŶŝ͕ŽŶŝŶŝ^͕ĞƚĂů͘ ůůĞƌŐŝĐZŚŝŶŝƟƐĂŶĚŝƚƐ/ŵƉĂĐƚŽŶƐƚŚŵĂ;Z/ͿŐƵŝĚĞůŝŶĞƐ͗ 2010 revision. J Allergy Clin Immunol 2010;126:466-476. ϭϲ͘ dŽƌƌĞƐ :͕ ĚĞ >ĂƐ ,ĞƌĂƐ D͕ DĂƌŽƚŽ ^͕ sŝǀĂŶĐŽ &͕ Ğƚ Ăů͘ Molecular and immunological characterization of the ĮƌƐƚ ĂůůĞƌŐĞŶŝĐ ůŝƉŽĐĂůŝŶ ŝŶ ŚĂŵƐƚĞƌ͗ ƚŚĞ ŵĂũŽƌ ĂůůĞƌŐĞŶ from Siberian hamster (Phodopus sungorusͿ͘:ŝŽůŚĞŵ ϮϬϭϰ͖Ϯϴϵ͗ϮϯϯϴϮͲϮϯϯϴϴ͘ ϳ͘ ^ĂŶƚŽƐ D^͕ ůǀĞƐ DZ͕ &ƌĞŝƚĂƐ ͕ ^ŽƵƐĂ >͕ Ğƚ Ăů͘ KĐƵůĂƌ ĂůůĞƌŐLJ >ĂƚŝŶ ŵĞƌŝĐĂŶ ĐŽŶƐĞŶƐƵƐ͘ ƌƋ ƌĂƐ KĨƚĂůŵŽů 2011;74:452-456. 17. Chapman JA, Williams S. Aeroallergens of the southeast DŝƐƐŽƵƌŝ ĂƌĞĂ͗ Ă ƌĞƉŽƌƚ ŽĨ ƐŬŝŶ ƚĞƐƚ ĨƌĞƋƵĞŶĐŝĞƐ ĂŶĚ Ăŝƌ ƐĂŵƉůŝŶŐĚĂƚĂ͘ŶŶůůĞƌŐLJϭϵϴϰ͖ϱϮ͗ϰϭϭͲϰϭϴ͘ ϴ͘ ,ĂŶŝĮŶ:D͘ŝĂŐŶŽƐƟĐĐƌŝƚĞƌŝĂĨŽƌĂƚŽƉŝĐĚĞƌŵĂƟƟƐ͗ĐŽŶƐŝĚĞƌƚŚĞĐŽŶƚĞdžƚ͘ƌĐŚĞƌŵĂƚŽůϭϵϵϵ͖ϭϯϱ͗ϭϱϱϭ͘ ϵ͘ ,ĞŝŶnjĞƌůŝŶŐ>D͕ƵƌďĂĐŚ':͕ĚĞŶŚĂƌƚĞƌ'͕ĂĐŚĞƌƚ͕Ğƚ Ăů͘';ϮͿ>EƐŬŝŶƚĞƐƚƐƚƵĚLJ/͗';ϮͿ>EŚĂƌŵŽŶŝnjĂƟŽŶŽĨ ƐŬŝŶƉƌŝĐŬƚĞƐƟŶŐ͗ŶŽǀĞůƐĞŶƐŝƟnjĂƟŽŶƉĂƩĞƌŶƐĨŽƌŝŶŚĂůĂŶƚ ĂůůĞƌŐĞŶƐŝŶƵƌŽƉĞ͘ůůĞƌŐLJϮϬϬϵ͖ϲϰ͗ϭϰϵϴͲϭϱϬϲ͘ ϭϴ͘ >ŝŶŶĂ K͕ EŝŝŶŝŵćŬŝ ͕ DćŬŝŶĞŶͲ<ŝůũƵŶĞŶ ^͘ /ŵŵƵŶŽůŽŐŝĐ ĐƌŽƐƐͲƌĞĂĐƟǀŝƚLJ ďĞƚǁĞĞŶ ŚĞŶ͛Ɛ ĨĞĂƚŚĞƌ ĂŶĚ ŚŽƵƐĞͲĚƵƐƚͲ ŵŝƚĞĂůůĞƌŐĞŶĞdžƚƌĂĐƚƐ͘ůůĞƌŐLJϭϵϵϰ͖ϰϵ͗ϳϵϱͲϳϵϲ͘ 10. De Knop KJ, Verweij MM, Grimmelikhuijsen M, Philipse E, ĞƚĂů͘ŐĞͲƌĞůĂƚĞĚƐĞŶƐŝƟnjĂƟŽŶƉƌŽĮůĞƐĨŽƌŚĂnjĞůŶƵƚ;Corylus avellana) in a birch-endemic region. Pediatr Allergy ImmuŶŽůϮϬϭϭ͖ϮϮ;ϭWƚϮͿ͗ĞϭϯϵͲϰϵ͘ ϭϭ͘ DĂƚƌŝĐĂƌĚŝWD͕ŽĐŬĞůďƌŝŶŬ͕ĞLJĞƌ<͕<Ğŝůd͕ĞƚĂů͘WƌŝŵĂƌLJ versusƐĞĐŽŶĚĂƌLJŝŵŵƵŶŽŐůŽďƵůŝŶƐĞŶƐŝƟnjĂƟŽŶƚŽƐŽLJ ĂŶĚǁŚĞĂƚŝŶƚŚĞDƵůƟͲĞŶƚƌĞůůĞƌŐLJ^ƚƵĚLJĐŽŚŽƌƚ͘ůŝŶ džƉůůĞƌŐLJϮϬϬϴ͖ϯϴ͗ϰϵϯͲϱϬϬ͘ 10 2016 enero-marzo;63(1) ϭϵ͘ <ĞŵƉd:͕^ŝĞďĞƌƐZt͕&ŝƐŚǁŝĐŬ͕K͛'ƌĂĚLJ'͕ĞƚĂů͘,ŽƵƐĞ ĚƵƐƚŵŝƚĞĂůůĞƌŐĞŶŝŶƉŝůůŽǁƐ͘D:ϭϵϵϲ͖ϯϭϯ͗ϵϭϲ͘ ϮϬ͘ ŽůůŽīD:͕DĞƌƌĞƩd'͕DĞƌƌĞƩ:͕DĐ^ŚĂƌƌLJ͕ŽLJĚ'͘&ĞĂƚŚĞƌ ŵŝƚĞƐĂƌĞƉŽƚĞŶƟĂůůLJĂŶŝŵƉŽƌƚĂŶƚƐŽƵƌĐĞŽĨĂůůĞƌŐĞŶƐĨŽƌƉŝŐĞŽŶ ĂŶĚďƵĚŐĞƌŝŐĂƌŬĞĞƉĞƌƐ͘ůŝŶdžƉůůĞƌŐLJϭϵϵϳ͖Ϯϳ͗ϲϬͲϲϳ͘ Ϯϭ͘ <ŝůƉŝƂ<͕DćŬŝŶĞŶͲ<ŝůũƵŶĞŶ^͕,ĂĂŚƚĞůĂd͕,ĂŶŶƵŬƐĞůĂD͘ ůůĞƌŐLJƚŽĨĞĂƚŚĞƌƐ͘ůůĞƌŐLJϭϵϵϴ͖ϱϯ͗ϭϱϵͲϭϲϰ͘ 22. Sanchez J, Diez S, Cardona R. Sensibilización a aeroalergenos en pacientes alérgicos de Medellín, Colombia. Rev ůĞƌŐDĠdžϮϬϭϮ͖ϱϵ͗ϭϯϵͲϭϰϳ͘