R EasyPRP KIT 2 1 10cc 1.5cc 0 cc Easy PRP 3 2 1 easy prp 3 4 5 1- Easy PRP Extraction Syringe 2- RBC Collector 3- Scalp Vein Set(Butterfly Set) 4- 3 ml Syringe and Syringe coupler 5- White Cap 10cc yP RP Anticoagulant Eas (ACD-A or Sodium Citrate) 10cc 1,5c c 10cc Eas yP RP 0 cc 10cc 0 cc Easy PRP Easy PRP c 1,5cc 1,5cc 0 cc 0 cc 0 cc 1,5cc Easy PRP 1,5cc Easy PRP 1,5c 1- Take 1,5ml ACD-A or Sodium Citrate anticoagulant into the EasyPRP syringe. 0 cc PRP CENTRIFUGE 4- Invert the EasyPRP syringe gently several times. 10cc 10cc 0cc 0cc 2-Take 8,5 ml blood from the patient by using a scalp vein set (butterfly set). (Draw the lower part of the piston to the level of 10 ml). 5- Insert the Easy PRP syringe into the centrifuge. Set up the program : Speed:2400 RPM (RCF:1200 G) Time: 5 Min.Start by pushing «START» 3- Attach the RBC (red blood cell) collector firmly into the EasyPRP syringe. Remove the plunger from the EasyPRP syringe by turning it counterclockwise. Insert the counterbalance into the centrifuge ,always opposite from the Easy PRP syringe.(If 1 or 3 Easy PRP syringes are used.) Easy PRP Easy PRP Easy PRP 10cc 10cc 10cc Easy PRP Easy PRP Easy PRP Easy PRP 1,5cc 10cc 0 cc 10cc 1,5cc 0 cc 0 cc 1,5cc 0 cc 1,5cc 1,5cc 0 cc 10cc Easy PRP 10cc Easy PRP 10cc PLEASE NOTE: RBC part pushed down to 0 ml line= with Buffy Coat All RBC removed (only clear serum)= without Buffy Coat (Take a firm hold of the plunger and the Easy PRP syringe and press firmly but carefully to prevent too much of RBC to be discharged.) 1,5cc 10cc 1,5cc 1,5cc 1,5cc 0 cc 0 cc 0 cc 0 cc 9- Remove the plunger and plug 7- Discharge the remaining RBC from the 8-Turn the Easy PRP syringe 6-Remove the syringe from the centrifuge the white cap firmly on the tip up-side down and release the Easy PRP syringe by pushing down the and connect the plunger back into the remaining air from the EasyPRP of the Easy PRP syringe plunger. Easy PRP syringe by turning it clockwise. syringe by pushing the content Carefully separate the RBC collector from up to the tip. the Easy PRP syringe by turning it clockwise. 10c 10cc PRP Easy PRP PPP 10cc 10cc Easy PRP 1,5cc 10cc Easy PRP Easy PRP cc c 10cc Easy PRP 1,5 0c 10cc Easy PRP Ea sy PR P c 1,5cc 1,5cc 1,5cc 0 cc 0 cc 0 cc 0 cc Buffy Coat 1 0 cc 2 1,5cc 3 0 cc 1,5cc PRP CENTRIFUGE 14- PRP is ready to be used. 1 1 1 2 2 11- Remove the syringe from centrifuge and connect the plunger back into the EasyPRP syringe.Carefully separate the white cap from the EasyPRP syringe. 12- Put the syringe coupler into Easy PRP syringe. Connect the 3 ml syringe. 1 2 3 3 3 ISO 10002:2004 Certification on Service Quality Management 2 NOTE: Keep the EasyPRP extraction syringe upright during the process of preparing PRP 3 10- Insert the Easy PRP syringe and counterbalance into the centrifuge. Set up the program: Speed:2400 RPM (RCF:1200 G) Time: 10 Minutes Start by pushing «START» 13-PRP can be transferred to the other syringe by pushing the Easy PRP plunger down-wards.