AVE-764 Service Manual AVE-764 Series Urine Formed Elements Analyzer Service Manual AVE SCIENCE & TECHNOLOGY CO., LTD. Building B6, Luguyuyuan, No.27 Wenxuan Road, Changsha Hi-tech Industrial Development Zone, Changsha, Hunan 410205, P.R.China 1 AVE-764 Service Manual The information in this manual was correct at the time of printing. However, AVE SCIENCE & TECHNOLOGY continues to improve its products and reserves the right to change specifications, equipment, and maintenance procedures at any time without notice. Companies, names and data used in examples are fictitious unless otherwise noted. No part of this document may be reproduced or transmitted in any form or by any means, electronic, mechanical or otherwise, for any purpose, without the express written permission of AVE. AVE may have patents or pending patent applications, trademarks, copyrights or other intellectual or industrial property rights covering this document or subject matter in this document. The furnishing of this document does not give a license to these property rights except as expressly provided in any written license agreement from AVE. If this instrument is used in a manner differently than specified in this manual, the protection provided by the equipment may be impaired. Service Manual for software version 2.2.8.2015 Modification history Version Date Modification 1.1 31.12.2013 First edition 1.2 31.10.2014 Updated for software version V2.1.12.2014 1.3 20.05.2016 Updated for software version V2.2.8 2015 2 AVE-764 Service Manual Index 1 INTRODUCTION ........................................................................................................................... 7 1.1 Intended Use ....................................................................................................................... 7 1.2 How to use the Service Manual .......................................................................................... 7 1.3 Precautions and Warnings Symbols – Definitions .............................................................. 7 1.3.1 Cautions .................................................................................................................... 7 1.3.2 Warnings ................................................................................................................... 7 1.3.3 Biological Warnings................................................................................................... 7 1.4 Symbols ............................................................................................................................... 8 1.5 Contact Information ............................................................................................................. 9 1.6 Limitation of Liability ............................................................................................................ 9 1.7 Use of Third Party Computer Products ............................................................................... 9 2 INSTALLATION ........................................................................................................................... 10 2.1 Summary ........................................................................................................................... 10 2.2 Installation Requirements .................................................................................................. 10 2.2.1 Space ...................................................................................................................... 10 2.2.2 Power ...................................................................................................................... 10 2.2.3 Environmental requirements ................................................................................... 10 2.2.4 Installation ............................................................................................................... 11 2.3 Shipping Cartons ............................................................................................................... 11 2.3.1 Packing List ............................................................................................................. 11 2.3.2 Unpacking the device .............................................................................................. 14 2.4 Installation the device ........................................................................................................ 21 2.4.1 Unscrew microscope fixing plate ............................................................................ 21 2.4.2 Adjust the level of the instrument ............................................................................ 22 2.4.3 Installing the automatic sampler ............................................................................. 23 2.4.4 Installing the Counting Chamber ............................................................................ 25 2.4.5 Installing the watch-dog .......................................................................................... 26 2.4.6 AVE-764 System Connection ................................................................................. 27 2.4.7 AVE-764 reagent connection .................................................................................. 27 2.4.8 Connecting the power cable ................................................................................... 28 2.4.9 Connecting Urine Analyzer ..................................................................................... 28 2.4.10 Power-on ............................................................................................................... 28 2.4.11 Adjustment ............................................................................................................ 28 2.4.12 Installation Checklist ............................................................................................. 32 3 SETUP ......................................................................................................................................... 33 3.1 LIS Setting ......................................................................................................................... 33 3.1.1 Data In Setting ........................................................................................................ 33 3.1.2 Data Out Setting...................................................................................................... 34 3.2 Barcode Setting ................................................................................................................. 35 3.3 System Setting .................................................................................................................. 36 3 AVE-764 Service Manual 3.3.1 COM Setting ............................................................................................................ 37 3.3.2 Microscopy Setting .................................................................................................. 37 3.3.3 Channel Setting....................................................................................................... 38 3.3.4 Image Setting .......................................................................................................... 40 3.3.5 Microscopy Setting .................................................................................................. 40 3.3.6 Morphology Setting ................................................................................................. 41 3.4 Operator Management ...................................................................................................... 41 4 AVE-764 ANALYZER .................................................................................................................. 43 4.1 Components ...................................................................................................................... 43 4.2 Power System ................................................................................................................... 48 4.2.1 Components ............................................................................................................ 48 4.2.2 Maintenance and Replacement .............................................................................. 48 4.3 Barcode Reader ................................................................................................................ 50 4.4 Cooling Fans & Dust Cover ............................................................................................... 52 4.5 COM Board........................................................................................................................ 54 5 AUTOMATIC SAMPLER ............................................................................................................. 55 5.1 Summary ........................................................................................................................... 55 5.2 Component ........................................................................................................................ 55 5.2.1 Function areas ........................................................................................................ 55 5.2.2 Electrical control system ......................................................................................... 56 5.2.3 Electric layout .......................................................................................................... 56 5.3 Adjustment ......................................................................................................................... 60 5.3.1 Mechanical testing .................................................................................................. 60 5.3.2 Reflective photoelectric sensor adjustment ............................................................ 60 5.3.3 Motor testing ........................................................................................................... 63 5.3.4 Correlation photoelectric sensor testing ................................................................. 64 5.3.5 Inching photoelectric sensor testing ....................................................................... 64 5.4 Maintenance ...................................................................................................................... 65 5.4.1 Daily maintenance of automatic sampler ................................................................ 65 5.4.2 Reflective photoelectric sensor maintenance ......................................................... 65 5.5 Replacement ..................................................................................................................... 66 5.5.1 Reflective photoelectric sensor replacement .......................................................... 66 5.5.2 Correlation photoelectric sensor replacement ........................................................ 66 5.5.3 Inching photoelectric sensor replacement .............................................................. 67 5.5.4 Main control board replacement ............................................................................. 68 5.5.5 Motor replacement .................................................................................................. 69 6 PUMP & VALVE SYSTEM MODULE .......................................................................................... 70 6.1 Summary ........................................................................................................................... 70 6.2 Components ...................................................................................................................... 71 6.2.1 Main control board (02A) ........................................................................................ 71 6.2.2 Extend Controller Module Components .................................................................. 72 6.2.3 Triple Valve Components ........................................................................................ 73 6.2.4 Wash pool ............................................................................................................... 73 6.3 Adjustment ......................................................................................................................... 74 4 AVE-764 Service Manual 6.3.1 Pump & valve testing .............................................................................................. 75 6.3.2 Pump-Valve Calibration .......................................................................................... 76 6.3.3 Blank Calibration ..................................................................................................... 78 6.4 Maintenance and servicing................................................................................................ 78 6.4.1 Quit and Maintain .................................................................................................... 78 6.4.2 Valve-tube Maintenance ......................................................................................... 78 6.4.3 Other pipelines Maintenance .................................................................................. 79 6.4.4 Counting Chamber Maintenance ............................................................................ 79 6.4.5 Wash pool Maintenance ......................................................................................... 79 6.5 Replacement ..................................................................................................................... 80 6.5.1 Peristaltic-pump tube replacement ......................................................................... 80 6.5.2 Disassembly diagram of the pumps and valves ..................................................... 84 6.5.3 Triple valve replacement ......................................................................................... 89 7 SAMPLING MODULE .................................................................................................................. 91 7.1 Summary ........................................................................................................................... 91 7.2 Components ...................................................................................................................... 91 7.3 Adjustment ......................................................................................................................... 92 7.4 Maintenance ...................................................................................................................... 94 7.5 Replacement ..................................................................................................................... 94 7.5.1 Replacing sampling control board (02C) ................................................................ 94 7.5.2 Replacing sampling pinboard (02D) ....................................................................... 95 7.5.3 Replacing back-forth photoelectric sensor.............................................................. 95 7.5.4 Replacing up-down photoelectric sensor ................................................................ 96 7.5.5 Replacing motors .................................................................................................... 97 7.5.6 Replacing sampling needle..................................................................................... 97 8 MICROSCOPE MODULE .......................................................................................................... 100 8.1 Summary ......................................................................................................................... 100 8.2 Components .................................................................................................................... 100 8.3 Microscope and Image Adjustment ................................................................................. 102 8.3.1 Preparation for microscope focus adjustment ...................................................... 102 8.3.2 Objective Adjustment ............................................................................................ 102 8.3.3 Square-Mark Adjustment of Microscope............................................................... 103 8.3.4 Focus Adjustment ................................................................................................. 104 8.3.5 Image Adjustment ................................................................................................. 105 8.4 Maintenance .................................................................................................................... 106 8.4.1 Cleaning of Counting-chamber and stage ............................................................ 106 8.4.2 Cleaning of Sensors .............................................................................................. 106 8.4.3 Cleaning of Micro-lamp and Focus lamp .............................................................. 106 8.4.4 Maintenance of rotation axis ................................................................................. 106 8.4.5 Maintenance of counting chamber........................................................................ 107 8.5 Replacement ................................................................................................................... 107 8.5.1 Replacement of Micro-lamp .................................................................................. 107 8.5.2 Replacement of Counting Chamber ..................................................................... 109 5 AVE-764 Service Manual 8.5.3 Replacement of CCD ............................................................................................ 113 8.5.4 Replacement of Sensors....................................................................................... 114 8.5.5 Replacement of microscope main control board .................................................. 117 9 TROUBLESHOOTING............................................................................................................... 118 9.1 Sampler error message ................................................................................................... 118 9.2 Pump-valve module error message ................................................................................ 122 9.3 Automatic sampler error message .................................................................................. 127 9.4 Microscope module error message ................................................................................. 128 9.5 Software error message .................................................................................................. 132 9.6 Other error message ....................................................................................................... 134 10 APPENDICES .......................................................................................................................... 136 10.1 Electrical Connection Diagram ...................................................................................... 136 10.1.1 Main Diagram ...................................................................................................... 136 10.1.2 Automatic Sampler Diagram ............................................................................... 137 10.1.3 Sampling Module Diagram.................................................................................. 138 10.1.4 Microscope Module Diagram .............................................................................. 139 10.2 Upgrading the software ................................................................................................. 139 10.2.1 Upgrading the software ....................................................................................... 139 10.2.2 Reinstalling the software ..................................................................................... 141 10.3 Upgrading the module firmware .................................................................................... 141 10.3.1 Module firmware requirements ........................................................................... 141 10.3.2 Upgrading the module firmware.......................................................................... 142 10.4 Installing or uninstalling the driver of CCD camera ....................................................... 143 10.5 Reattaching and reinstalling the Database ................................................................... 143 10.5.1 Reattaching the Database .................................................................................. 143 10.5.2 Reinstalling the Microsoft SQL Server ................................................................ 147 10.6 Backup and restoring the Operation System ................................................................ 147 10.6.1 Backup the operation system.............................................................................. 147 10.6.2 Restoring the Operation System ......................................................................... 150 10.7 Maintenance kit ............................................................................................................. 153 10.8 Recommended spare parts ........................................................................................... 153 10.9 Operating AVE-764 and urine analyzer together .......................................................... 156 6 AVE-764 Service Manual 1 INTRODUCTION 1.1 Intended Use The AVE-764 is a fully automated analyzer for measuring formed elements in urine. The analyzer is designed specifically for professional use, as an In Vitro Diagnostic (IVD) device used by qualified physicians and laboratory staff. The analyzer is intended for use in screening to assist diagnosis for urinary system diseases, kidney diseases, circulatory system diseases or infectious diseases. The AVE-764 can be used as a stand-alone device for microscopic analysis, and the results from the AVE-764 can also be combined with urine chemistry results in an integrated report. 1.2 How to use the Service Manual The Service Manual is intended to explain the instrument in detail, to be a training guide for authorized technician and to be a troubleshooting reference manual. This manual is designed for authorized technician who will maintain and repair the analyzer and must have a thorough knowledge of it. It also specifically references information concerning analyzer components, operation, theory, utility and performance. 1.3 Precautions and Warnings Symbols – Definitions The Service Manual includes information and warnings that must be observed by the user to ensure a safe operation of the system. 1.3.1 Cautions CAUTION: Identifies situations that could lead to an accident or equipment failure. CAUTION: Identifies electrical caution! Unplug before handling. CAUTION: Identifies laser light. A laser is used to read the barcodes only. Protect eyes from the laser light. 1.3.2 Warnings WARNING: Identifies potentially hazardous situations that could result in serious injury to laboratory personnel. 1.3.3 Biological Warnings Identifies potential biological hazards; please be cautious when using, cleaning or maintain the equipment and take appropriate protective measures. 7 AVE-764 Service Manual 1.4 Symbols The following is a list of symbols used on the nameplate, product packaging or within the Service Manual and their corresponding meaning. NO. Symbol Description Location Service Manual, nameplate Service Manual, Instrument Service Manual, Instrument 1 Cautions 2 Cautions 3 Cautions 4 Warnings Service Manual 5 Biological hazards Service Manual, Instrument 6 Earth terminal, the terminal must be connected firmly Instrument 7 Power on Service Manual 8 Power off Service Manual 9 Date of manufacture Nameplate 10 In vitro diagnostic medical device Nameplate 11 Manufacturer Nameplate 12 CE Mark Nameplate 13 Consult instructions for use Product packaging 14 Fragile Product packaging 15 Do not use when package damaged Product packaging 16 Stacking limits Product packaging 17 Ban rolling Product packaging 18 Upward Product packaging 8 AVE-764 NO. Symbol Service Manual Description Location Keep dry Product packaging 19 1.5 Contact Information AVE SCIENCE & TECHNOLOGY CO., LTD. Building B6, Luguyuyuan, No.27 Wenxuan Road Changsha Hi-tech Industrial Development Zone, Hunan 410205, P.R.China Tel: +86-731-8892 5308 Fax: +86-731-8890 7046 Website: http://www.c-ave.com/en.html 1.6 Limitation of Liability AVE shall not be liable for any loss of use, revenue or anticipated profits, or for any consequential or incidental damages resulting from the sale or use of the products. 1.7 Use of Third Party Computer Products AVE does not recommend that the computers used as a functional part of the AVE-764 be employed for performing any software or hardware based applications other than those specifically furnished to operate and support the AVE-764 system, or those recommended and offered by AVE specifically as accessories or enhancements for the AVE-764 system. No other third party application software should be installed in these computers in addition to those provided or recommended by AVE, without the expressed approval of AVE Technical Service, in order to avoid potential performance and reliability problems which can result from incompatibility factors, errors in use of such software, or software-based “viruses.” Installation of such third party software, or non-approved electronic cards or other devices, without advance approval by AVE may affect the terms of or void any warranty otherwise in effect, covering AVE-764 supplied software and hardware on the computers and the overall performance and reliability of the entire AVE-764 system. 9 AVE-764 Service Manual 2 INSTALLATION 2.1 Summary Anyone who is not trained by AVE or authorized distributors of AVE may cause damage to the analyzer when opening the box or installing the instrument. Therefore do not open the box or install the instrument without an authorized technician being present. On receiving the analyzer, please check the package carefully to see whether there is a physical damage. If there is any, please notify your local distributor immediately. 2.2 Installation Requirements 2.2.1 Space The AVE-764 is a tabletop unit which main unit size is 680(L) x 650(W) x 545(H) mm and main unit weight is 63kg. Instrument is requiring free space on a countertop of approximately 150x120 cm, with vertical clearance of at least 30 cm. 2.2.2 Power Voltage Frequency Input power Fuse 100-240V~ 50/60Hz ≤450VA T3.15A 250V Φ5×20 CAUTION: Insure AVE-764 use dedicated power source, this power source does not supply other instrument and equipment. CAUTION: UPS no less than 1500VA or voltage regulator are recommended for the AVE-764 to maintain system operation if voltage is not stable or power outage is regular. CAUTION: The equipment inside fixed covered areas of the instrument are not user serviceable and may present electrical shock hazards. WARNING:For continued protection against risk of fire and hazard, replace only with the same type and rating fuse. WARNING:Equipment requires connection to protective earth for safety reasons. WARNING:The instrument’s main supply inlet is being used as the mains disconnect device. 2.2.3 Environmental requirements The instrument is suggested to be installed in the environment as: • Normal working temperature: 15°C – 30°C 10 AVE-764 Service Manual • Relative humidity:20% - 80%; • Atmospheric pressure:76 – 106kpa; • Do not get close to strong electromagnetic source as not to affect the normal operation of equipment. • Keep the environment as clean as possible. Keep it free from mechanical vibration, noise and power interruptions. • Avoid direct sunlight or exposure to heat or wind. 2.2.4 Installation CAUTION: The AVE-764 has to be installed by an authorized technician. 2.3 Shipping Cartons 2.3.1 Packing List AVE-764 package contains 2 boxes: plywood box is main unit package; carton box is accessories package. 1 Fig.2-1 Main unit Package 11 AVE-764 Service Manual 5 2 6 7 3 4 Fig. 2-2 Accessories package AVE-764 Packing List Analyzer (including computer, microscope) 1 Automatic sampler Watch dog Counting chamber with bracket Vent cover Keyboard & Mouse 10X & 40X Lens LCD monitor VGA cable 2 Power cable Monitor base User guider of monitor 12 AVE-764 Service Manual AVE-764 Packing List Laser printer 3 USB cable Power cable User guide Driver CDROM Warranty card Printing paper Test tube racks Test tube 4 Power cable Waste detector 5 Dustproof sponge Pump tubes Waste tube Φ4×Φ6-3000mm Cleanser tube (Silicone tube -1500mm) COM cable PS2 extension cable / VGA extension cable Reagent COM cable Reagent container cap (Cleanser A, Cleanser B & maintenance Cleanser) Microscope lamp Fuse(T3.15A Φ5×20) Label Sample needle stylet Counting chamber(3 channels) Windows7 system software discs Socket head wrench 13 AVE-764 Service Manual AVE-764 Packing List Cleanser A Container 6 Waste Container Maintenance Cleanser Container 7 Cleanser B Container 2.3.2 Unpacking the device CAUTION: Please check the package carefully to see whether there is damage or package has been open before. If there is any, please take pictures as evidence and notify AVE service engineer immediately. 2.3.2.1 Unpacking the main unit package 1. Loosen all of screws in the cover plate and take them away, then open the cover plate; 14 AVE-764 2. 3. Service Manual Fig. 2-3 Take the cover plate away Take away EPE foam coved on the Automatic sampler, then take out the box packed counting chamber with bracket and watch-dog, which stick under the EPE form, stay for installation use. Fig.2-4 Take out sampling cover plate and counting chamber Take out Automatic sampler stay for installation use, and take away the two pieces upper padding foam on the instrument. 15 AVE-764 Service Manual 4. Fig. 2-5 Take out Automatic sampler and upper padding foam Loosen all of screws in two side plates and take these plates away. 5. Fig. 2-6 Take away two side plates Take out instrument and place it on a flat table, then prepare installation. CAUTION: It takes two adults to lift the AVE-764 main unit onto the table. 16 AVE-764 Service Manual Fig.2-7 Take out instrument 6. 7. Fig.2-8 Bottom plate In order to prevent damage to equipment parts due to handling or vibration during transportation, microscope and CPU fan are all added EPE foam pads, please take them away before installation. Open the front cover and left side panel (face to the instrument), take out protection pads No.2 which installed on CCD upper side. 17 AVE-764 Service Manual 8. Fig.2-9 Front View Take out the protection pad No.1 which fixed under microscope stage. 9. Fig.2-10 Under microscope stage Install the 10X and 40X Lens to the microscope. 18 AVE-764 Service Manual Fig.2-11 10X and 40X Lens Fig.2-12 Install 10X and 40X Lens 10. Remove the security holder to fix the sample needle. 19 AVE-764 Service Manual Fig.2-13 Security holder for sample needle 11. Open right side panel, take out protection pad No.3 under CPU fan. 20 AVE-764 Service Manual Fig.2-14 Under CPU fan 12. Removed package material such as bottom plates, side plates etc., which stored together for instrument transfer and repack use; 2.3.2.2 Accessories Package Open Step Open box and take out all of accessories, check them according to the packing list. If there has any question please contact local distributor. All accessories should be place properly for installation and maintenance change use. 2.4 Installation the device For security, we suggest you read these operating instructions carefully and make corresponding precautions. 2.4.1 Unscrew microscope fixing plate Microscope is instrument precision part and in order to prevent damage to microscope due to handling or vibration during transportation, which fixation with two fixing plates before leave factory. Please unscrew these two fixing plates before installation. Operation steps as follows: Loosen the 4 screws, open left panel. Face instrument, open the front panel, can see the two fixing plates under microscope both ends. 21 AVE-764 Service Manual Loosen the 4 screws on the front-end of microscope cover plate by long handle cross screwdriver, but do not remove them.. Refer to step 3 loosen the 4 screws in the rear, but do not remove them; install the left side panel back. CAUTION: When transporting the instrument, make sure to tighten all screws. 2.4.2 Adjust the level of the instrument 1. 2. 3. Make certain that the bench top is level There are four feet at the bottom of the instrument on the four corners. Adjust the height of the feet to keep the instrument in level. Rotate counterclockwise to descend (as shown in fig identify direction), and clockwise for ascending. Make sure all the four feet are in contact the bench top with no suspension. 22 AVE-764 Service Manual Fig. 2-15 Adjust feet 2.4.3 Installing the automatic sampler 1. After level adjustment, place the automatic sampler in front of the main unit for about 12 cm, and then connect the power supply and communication cable of the sampler (shown in Fig. 2-16) to the interface on the sampler, as shown in Fig. 2-17. Fig. 2-16 Power supply and communication cable of sampler 23 AVE-764 2. 3. Service Manual Fig. 2-17 Connecting the power supply and communication cable Move the sampler towards the main unit until the tenon balls on each side of it are in alignment with the mortises on the sampler, and the fixing spindle at the lower middle of the main unit points the fixing hole of the latter, then slot them together. The tenon balls and fixing spindle are shown in Fig.2-18. Fig.2-18 Fixing sampler Adjust the feet at the bottom of the sampler to make certain the bottoms of the main unit and the sampler are of the same height. The adjustment is the same as that of the main unit. 24 AVE-764 Service Manual Fig. 2-19 Instrument 2.4.4 Installing the Counting Chamber In order to protect the Counting Chamber from damage during transport, it has been taken apart from the microscope and kept in a dedicated box before transport. Install counting chamber with bracket on the microscope stage before instrument working. 1. Open instrument front cover, take away 3 white counting chamber connecting plastic pipes, and take out the counting chamber with bracket from package, then take away silicone caps which covered on the counting chamber both ends. 2. There are 6 joints on the counting chamber 3 channels both ends, joint them with counting chamber pipelines according to color-ring separately. Pay attention to that pipeline should insert to joints 1/2 place, as shown in the figure below. The counting chamber has one side with two location holes which should be installed inwards. 25 AVE-764 3. Service Manual Fig. 2-20 Tubing connection of the Counting Chamber Clean up the microscope stage, then push the bracket under microscope piece and allow the two steel balls at both ends of the piece falling into the pits on each side of the bracket. Make certain the bracket is installed level without any looseness. Fig.2-21 Fixation counting chamber bracket 2.4.5 Installing the watch-dog Insert the watch-dog to any USB interface on the back of the instrument. As shown in the fig. 26 AVE-764 Service Manual Fig.2-22 Installing the watch-dog 2.4.6 AVE-764 System Connection For stand-alone use, the AVE-764 analyzer connects with other peripherals as shown in follows Figure 2-23. Ensure connecting in place and firmly. Fig. 2-23 AVE-764 analyzer and its peripheral connections 2.4.7 AVE-764 reagent connection WARNING: Operator has the duty to comply with the relevant regulations set by the region and country about the treatment and discard of expired reagents and wastes. WARNING: Reagents may cause some irritant to skin and eyes. Operator should always comply with the safety operation of laboratory and wear personal protective equipment (such as protective clothing, gloves, etc.)when coming contact reagents. CAUTION: Do not place reagent containers on the Analyzer. 27 AVE-764 Service Manual For stand-alone use, the AVE-764 analyzer is connected with reagent bottles as shown in Figure 2-24. Ensure connecting in place and firmly. Fig. 2-24 AVE-764 reagent connection 2.4.8 Connecting the power cable Plug one end of the provided power cord into the power inlet on the back of the host, and the other end into the dedicated connection board. To ensure safety, please set the power switch to “ ”, namely the instrument in a power-off state, and the socket is also in “off” state before connection. Connect the power cord of monitor and printer as the same way. 2.4.9 Connecting Urine Analyzer See 10.9 Operating AVE-764 and urine analyzer together. 2.4.10 Power-on Power-on operation order of AVE-764: 1. Turn on the UPS (if any). 2. Turn on the power distribution board (if any). 3. Turn on the power switches of printer, monitor, etc. 4. Turn on the main power switch(Green switch on slide, if it’s on, the yellow LED inside of the Green switch will be lighted). 5. Press the Start button on front-side to enter Windows OS. 2.4.11 Adjustment Every modules of the instrument will make an initialization after powering on, and any alarm, abnormal noises, shaking or mechanical failure should not be found during this processing. The correct processing of initialization should be like below. 1. The original condition of sampling needle is inserting in the wash pool. The initialization of Automatic sampler should follow this: Once the sampling needle is not 28 AVE-764 Service Manual at original condition, it will move up to its vertical zero position then back to its horizontal zero position. Finally it will move down to its original position. Once the sampling needle is at original condition, it will move up to its vertical zero position then forward and backward to perform horizontal setup. Finally it will move down to its original position. 2. The initialization of microscope module is intended to setup the five motors (up-down motor, left-right motor, forward-backward motor, objective motor and Focus lamp motor) to zero position. If they are already at the original condition, they will not move. Once any unusual conditions were found, please refer to above processing for trouble clearance. CAUTION: The processing of initialization mentioned above only be found when the instrument is power on, it’s different with the initialization after login the software. Accessing the Adjustment screen After initialization, you need to check and, if necessary, adjust every module following the sections below. You may need to login the Adjustment screen, which is a screen for service personnel to test the functionality of the subsystems of the device, and to adjust part of the preset parameters that control the operation of systems and subsystems. 1. Launch the system; 2. Log-in screen will pop up after launching. The default name ‘Service’ is visible on the screen, enter ‘password’ (without the inverted commas) as password. Then click Log in to login. 3. Click Management-Adjustment to enter Adjustment screen. Fig. 2-25 Adjustment screen 29 AVE-764 Service Manual 2.4.11.1 Sampling Module The Sampling Module consists mainly of sampling arm and pump-valve module. Adjusting the sampling arm 1. Launch system. 2. Prepare one tube of RBC suspension and place it on the tube rack. 3. Go to Management-Adjustment, click Wash, Wash+, Spe-Wash, Wash All buttons in turn. Repeat those operations three times to check if all the operations are correct. 4. Select 1#WPC 1#SPC 2#WPC, 2#SPC, 3#WPC, 3#SPC at pump calibration area then click Pump button in turn to get the Wash Time and Sample Time of three channels respectively. Repeat those operations above three times to check if all the operations are correct. Also check whether the Wash Time can meet its criterion between 3.5~5s, and Sample Time can meet its criterion between 4~5.5s. If the results are correct, go to step 5. If the results deviate from criterion. See 6.3 Adjustment for further adjustment. 5. Load the tube rack on ready area of sampler. Click Sample button and wait the rack be shifted to sampling area. Observe the movement of sampling needle, check whether the sampling location is right in the middle of tube and pinpoint of needle is 5mm above the bottom of tube. If the location deviates from the standard, see 7.3 Adjustment for further adjustment. 2.4.11.2 Automatic sampler After installing the Automatic sampler, you need to check its performance to make sure it running properly and prevent undetected situation. CAUTION: Follow the notes below during installing or testing. 1. Load the rack as close as possible to the right side of ready area. 2. Once the racks were loaded on the Automatic sampler, please do not move or take away the racks manually in case of unnecessary error. Adjusting the Automatic sampler 1. Launch system. 2. Prepare three tubes of RBC suspension and place them on the tube rack at regular intervals. 3. Go to Management-Adjustment and load the rack on the ready area. Click Sample All button, the rack will be pushed at testing area, and then it will be shifted by two pawls and will be stopped when the first test tube in the rack reaches at sampling location. After sampling from the first tube, the rack will be shifted again to finish the next two tubes sampling. After that the rack will be stopped at the last test tube. Click Sampler button, the rack will be shifted out from testing area by pawls and be pushed to tested area. Meanwhile the pawls continue moving to finish this setup. Repeat above operation five times to check ensure the Automatic sampler running properly. 4. Quit Adjustment screen and login routine work screen. Click Start button to start initialization for self-testing the status of every module. The Automatic sampler is also 30 AVE-764 5. 6. Service Manual tested by itself. After this, check whether the status of Automatic sampler is Normal in the Status area. Under working status, push the empty carrier without tube insert arrived at sampling location of STAT module. Check whether the Insert STAT window will be jumped out in the screen. If the window is jumped out, see 5.3.4 Correlation photoelectric sensor testing to adjust STAT detection photoelectric sensor. On the other hand, push the carrier with tube insert arrived at sampling location of STAT module and check whether the window will be jumped out. If the windows is not jumped out, see 5.3.4 Correlation photoelectric sensor testing to adjust STAT detection photoelectric sensor (J10). Fig. 2-26 Insert STAT Under working status, prepare five tubes of RBC suspension and place them on the tube rack at regular intervals. Load the rack on the Automatic sampler to finish all examination. After examination, go to Data-Tube Records-Urine Tube Records to check whether there is any samples were undetected. If there is, see 5.3.2 Reflective photoelectric sensor adjustment to Input end detection photoelectric sensor (JR8). 2.4.11.3 Microscope Module After installation, you need to check the performance of microscope to make sure it running properly and if necessary, to adjust the mark position, focus and quality of image so that to get clearer images and accurate results. Adjusting the Microscope Module 1. Launch system. 2. Prepare three tubes of RBC suspension and place them on the tube rack at regular intervals. 3. Go to Management-Adjustment and load the rack on the ready area. Click Sample All button to intake sample into three channels. Check whether there is bubble exists in the channels. See 8.3.3 Square-Mark Adjustment of Microscope to adjust the square mark to initial position (see below). If the mark can not be adjusted to initial position in any way, see 8.3 Microscope and Image Adjustment for further adjustment. 4. Select three channels in turn and click Auto-Focus button to perform focus auto-adjustment. Check whether the cells are clear. Re-perform auto-adjustment until the cells are clear and can be identified. Then click save button to save the adjusted 31 AVE-764 5. 6. Service Manual values. If the focus in any way can not be adjusted well, see 8.3 Microscope and Image Adjustment. After adjusting the focus of three channels, Click Wash All button and then quit the Adjustment screen. Login routine work screen. Click Start button to start initialization to self-test the status of every module. The microscope module is also tested by itself. After this, check whether the status of microscope is Normal in the Status area. 2.4.12 Installation Checklist Laboratory Serial number of device Service engineer Date Procedure Service manual reference Unpacking the device 2.3.2 Unpacking the device Check Packing List 2.3.1 Packing List Unscrew microscope fixing plate 2.4.1 Unscrew microscope fixing plate Adjust the level of the instrument 2.4.2 Adjust the level of the instrument Installing the automatic sampler 2.4.3 Installing the automatic sampler Installing the Counting Chamber 2.4.4 Installing the Counting Chamber Installing the watch-dog 2.4.5 Installing the watch-dog AVE-764 System Connection 2.4.6 AVE-764 System Connection AVE-764 reagent connection 2.4.7 AVE-764 reagent connection Connecting the power cable 2.4.8 Connecting the power cable Connecting Urine Analyzer(as needed) 2.4.9 Connecting Urine Analyzer Power-on 2.4.10 Power-on Adjustment of Sampling Module 2.4.11.1 Sampling Module Adjustment of Automatic sampler 2.4.11.2 Automatic sampler Adjustment of Microscope Module 2.4.11.3 Microscope Module 32 Comments √ AVE-764 Service Manual 3 SETUP The settings in this manual only can be modified by authorized technicians, the other settings permitted for the administrator and operator can refer to the user manual. CAUTION: This chapter is only used for authorized technicians. CAUTION: Since the setting will directly affect the instrument, once change, do check out this chapter or contact the service engineer. 3.1 LIS Setting Select “LIS setting” in the “Management” menu to open the LIS setting screen CAUTION: Contact AVE service engineer if you cannot realize LIS communication using the contents descript below. Fig. 3-1 LIS setting screen 3.1.1 Data In Setting Clicking on “Data In Setting” to open the setting screen for reading LIS data. 33 AVE-764 Service Manual Fig. 3-2 Data In Setting Data In Setting screen Instructions: Database information: Set DB string, type, address and data configuration list. DB Connection S: Set DB connection string. DB Type: Select the type of LIS DB to be input. The default DB are SQL Server and Oracle. DB Address: Set the address of LIS DB to be input. DB /Server Name: Set the name or server of LIS DB to be input. User: Set the name of LIS to be input. Password: Set the password of LIS to be input. Save: Save the settings. Delete: Delete existing connection information. String Mapping: Set LIS String Mapping. The existing LIS String is default. User can configure on it. LIS Table: Set LIS Table. LIS String: Set LIS String. Analyzer Table: Set Mapping Analyzer name. Analyzer String: Set Mapping Analyzer String. Remark: Set notes. Set Data in List 1. Set Connection string, complete DB type, address, DB /Server Name, user and password setting. Click Save to save settings.; 2. Right click to add a new LIS Table in String Mapping, entering String corresponding to the analyzer to accomplish String Mapping. 3. Click Save to save settings. Please refer to step 2 for operations when need to add or modify the settings. 3.1.2 Data Out Setting Click on Data Out setting in Management menu can realize the setting of the data output to LIS. 34 AVE-764 Service Manual Fig. 3-3 LIS Setting screen LIS connection setting is mainly for the connection of AVE-764 to LIS in hospitals. It can be used for data-sending-mode selection and serial port setting. Check connection mode, LIS COM on the right should be filled out if Export to COM is checked. Data Out Setting screen instructions: Mode: Select the data output mode. “Read Xml File”, “Read Database” and “Export to COM” can be checked. V Upload: Sample status shows as "V" or verification is saved, the data and results will be transmitted to LIS system automatically. All Upload: Any sample status or as soon as the testing results come out, the data and results will be transmitted to LIS system automatically. COM: Refer to the serial port setting between AVE-764 and LIS. Bit rate: Refer to the baud rate used for communication between AVE-764 and LIS. The default value is 9600 bits. Data: Refer to the data bits used for communication between AVE-764 and LIS. 8 data is the default value. Parity: Refer to the parity bit used for communication between AVE-764 and LIS. The default parity is “N”. Stop: Refer to the stop bit used for communication between AVE-764 and LIS. The default value is “1”. CAUTION: Click Save after settings have been altered to confirm. 3.2 Barcode Setting Select Barcode Setting in the Management menu to open the barcode setting screen. Fig.3-4 Barcode Setting screen 35 AVE-764 Service Manual Barcode Setting screen Instructions: 1. Check the Use Barcode box to use the barcode scanning feature. The system will take the barcode as the primary matching criterion for chemistry and microscopy results. 2. The system check internal as default for choosing the integrated barcode reader of the AVE-764. 3. The system check auto as default for choosing the integrated barcode reader of the AVE-764. 4. Check Tooltip window box to open it. It will pop up for barcode manual entry when the instrument fails to scan the barcode automatically. The Tooltip window won’t open when the instrument fails to scan the barcode without checking the box. 5. The system Check Barcode matching box as default for choosing the integrated barcode reader of the AVE-764. Barcode matching function will only take effect when the AVE-764 operating with a urine analyzer together. 6. Click Save to save settings. 7. Click Quit to exit. 3.3 System Setting Select System Settings in the Management menu to open six secondary submenus: COM Setting, Microscopy Setting, Channel Setting, Image Setting, Microscope Setting and Morphology Setting. Click one of them to enter the corresponding setting screen. Fig.3-5 System Settings screen 36 AVE-764 Service Manual 3.3.1 COM Setting Select “COM Setting” to open the COM setting screen. On this screen, the communication serial port settings between the master computer and each internal function module or external urine chemistry analyzer can be set. CAUTION: The serial port parameters are set on default, no modification is recommended. Fig. 3-6 COM setting screen Operations: 1. Select the parameter options on the left; 2. Select each parameter in COM, Bit rate, Data, Parity, and Stop box on the right. 3. Click Save after settings done. 4. Click Quit to quit the screen. 3.3.2 Microscopy Setting Select Microscopy Setting to open the Microscopy Setting screen. CAUTION: The microscopy parameters are set on default, since the settings will directly affect the test results, no modification is recommended, or may cause instrument failure. 37 AVE-764 Service Manual Fig. 3-7 Microscopy Setting screen Microscopy Setting screen Instructions Screen time: Set the time between fillings of the cell with the sample to the start of the primary screen. Deposit time: Set the time between fillings of the cell with the sample to the start of the LP scan. Neg. target: Set the particle number upper limit for negative specimen. High-pos. target: Set the particle number lower limit for strong positive specimen. Screen Frames: Set the primary screen frames. LP Frames: Set the LP screen frames for common specimen. HP Frames: Set the HP screen frames for common specimen. LP Frames (HpT): Set the LP screen frames for strong positive specimen as given by High-pos target. HP Frames (HpT): Set the HP screen frames for strong positive specimen as given by High-pos target. Mixing: Set the number of times the sample is mixed. Dilution: Setting is reserved until diluted samples are used for microscopy. It is recommended to use native urine and leave the factor set to 1. Centrifuge: Setting is reserved until centrifuged samples are used for microscopy. It is recommended to use native urine and leave the factor set to 1. Operation: 1. Enter appropriate data into the corresponding box in the Microscopy Setting screen. 2. Click Save after entries are done. 3. Click Quit to quit the Microscopy Setting screen. 3.3.3 Channel Setting Select Channel Setting to open the Channel Setting screen. CAUTION: Only authorized technicians can modify channel parameters, 38 AVE-764 Service Manual since the modification affects the test results directly! Fig. 3-8 Channel Setting screen Channel Setting Screen Instructions: Sample: Set the aspiration volume of the sample. Channel: Set the sample volume filling the Counting Chamber. Mixing in: Set the mixed sample aspiration volume. Mixing out: Set the mixed sample drained away volume. Wash: Set the washing volume of cleanser A for a single wash. Wash +: Set the washing volume of cleanser A for a single reverse wash. General: Set the washing volume of cleanser A for a single wash of the sampling needle outer wall. Maintain: Set the volume of maintenance cleanser for a single maintenance. Sample Time: Set the sample time of the peristaltic pump. Wash Time: Set the wash time of the peristaltic pump. LP coefficient: Set the LP coefficient for each channel. It has been set in the factory and is not recommended for change. Dirty spot: Set the dirty spot number for each channel. It can be set manually or through Blank Calibration automatically (See Section 7.4 Blank Calibration for details). Close CH: Choose to close the channel that may have some problems, especially when a channel of counting chamber is broken. Operations: 1. Enter appropriate data into the corresponding box in Channel Setting screen. 2. Check the channel for open or close. 3. Click Save after entry. 4. Click Quit to exit the screen. 39 AVE-764 Service Manual 3.3.4 Image Setting Select Image Setting to open the Image Setting screen. CAUTION: Only authorized technicians can modify channel parameters, since the modification affects the test results directly! Fig.3-9 Image Setting screen Operations: 1. Enter appropriate data into the corresponding box in the Image Setting screen. 2. Drop down the Format box to select image format. 3. Click Save after entry. 4. Click Quit to exit the screen. 3.3.5 Microscope Setting Fig.3-10 Microscope setting screen Select Microscope to open the Microscope screen. CAUTION: All items except Focus Protect have been set in the factory, no modification is recommended, any change may affect the test results directly, only authorized technicians can modify channel parameters! Focus Protect is to set the interval time between the Focus lamps shut in standby mode, 40 AVE-764 Service Manual the default is 30 min., and the time can be set shorter to extend the lamp life. Operations: 1. Enter appropriate data into the corresponding box in Microscope Setting screen. 2. Click Save after entry. 3. Click Quit to exit the screen. 3.3.6 Morphology Setting Select Morphology Setting to open the Morphology Setting screen. CAUTION: Only authorized technicians should modify morphology parameters, since the modification affects the test results directly! Fig. 3-11 Morphology Setting screen Operations: 1. Enter appropriate data into the corresponding Curve box in Morphology Setting screen. 2. Select specific X Curve and Y Curve in the Scattergraph area. 3. Check the items need to be printed in the Print area. 4. Click Save after setting. 5. Click Quit to exit the screen. 3.4 Operator Management Operator Management can provide various role authority management and distribution and ensure the parameters of the software run properly through assigning rights to different level users. Click Management - Operator Management to open the Operator Management screen. Click the “>/<” button in the middle of the screen to assign role rights. Click OK for confirming. Click Cancel to quit. 41 AVE-764 Service Manual Fig. 3-12 Operator Management screen CAUTION: If a submenu in “Management” is assigned to the role, it should be guaranteed that “Management” has been assigned to same role; otherwise the submenu cannot be displayed and used. 42 AVE-764 Service Manual 4 AVE-764 ANALYZER 4.1 Components The AVE-764 mainly consists of an analyzer, PC peripherals (monitor, mouse and keyboard) and other attachments (printer for example). The analyzer is composed of Microscope Module, Pump & Valve System Module, Sampler Module, Automatic sampler and PC Analysis Processing Modules, also includes Power System, Barcode Reader and Cooling Fans etc. auxiliary devices. Fig. 4-1 Front view of the analyzer Key to figure 4-1 1. Automatic sampler; 2. Microscope observation window; 3. Start button; 4. Reset button; 5. Power LED; 6. Harddisk LED; 7. Barcode Reader 43 AVE-764 Service Manual Fig.4-2 Back view of the analyzer Key to figure 4-2 1. Cooling fans; 2. COM7, Backup COM port; 3. COM8, Bridge connection port; 4. PC communication ports; 5. Main power input (fuse inside); 6. Waste COM port; 7. Reagent COM port; 8. Cleanser A connector; 9. Maintenance Cleanser connector; 10. Cleanser B connector; 11. Waste connector 44 AVE-764 Service Manual Fig. 4-3 Shaft side view of the analyzer Key to figure 4-3 1. Main power switch; 2. Tested area; 3. STAT area; 4. Ready area Fig. 4-4 Front view of the analyzer (front panel removed) Key to figure 4-4 45 AVE-764 Service Manual 1. Sampling module; 2. Magnetic valve pinboard; 3. Pump-valve Main control board; 4. Barcode reader Fig. 4-5 Back view of the analyzer (back panel removed) Key to figure 4-5 1. Cooling fan; 2. PC power module; 3. Microscope Module; 4. Main Power module; 5. Wash pool 46 AVE-764 Service Manual Fig. 4-6 Left view of the analyzer (left panel removed) Key to figure 4-6 1. Main Power module; 2. Pump-valve bracket assembly; 3. Main power switch Fig. 4-7 Right view of the analyzer (right panel removed) Key to figure 4-7 47 AVE-764 Service Manual 1. Triple solenoid valves assembly; 2. Hard disk; 3. PC motherboard; 4.2 Power System 4.2.1 Components The power system include one power switch and two power modules; the power modules located on the rear of the plate, fixed in the middle of instrument (Fig.4-5), one is PC power module, another is main power module, both are wide input, AC-DC power modules. The power switch is a green rocker switch on the left of instrument; it’s the main power switch of the AVE-764. PC power module is the general computer’s power module, supplying the power for the computer system of instrument through the standard power connector, and also supplying power for cooling fans and microscopy lamps with 12 V output; Main parameters: Input Voltage: 100-240V~ ,50/60Hz; Output Power: 400W; The main power module supplies power for analyzer’s modules through adapter (mainly include Sample Module, Microscope Module, Automatic Sample Module), excepting Computer System. Main parameters: Input Voltage: 100-240V~ ,50/60Hz; Output Power: 150W; Output Voltage: +5V±10%,+24V±10%; 4.2.2 Maintenance and Replacement CAUTION: The power system is dangerous, be careful of electric shock! If the power system runs error, firstly, to check the power module has power input or not; If works properly, disconnect the load cables of power system, then use the multimeter to measure the power input conforms the norminal voltage or not at the state of power-on. If not, the power module has to be replaced (Fig. 4-8 to Fig. 4-11). 48 AVE-764 Service Manual Fig. 4-8 PC power module Key to figure 4-8 1. PC power module; 2. M3X8 Phillips screw Fig. 4-9 Main Power Module Key to figure 4-9 1. Main Power Module; 2. M3X8 Phillips screw 49 AVE-764 Service Manual Fig. 4-10 Power Module with mounting plate Key to figure 4-10 1. M3X6 Phillips screw; 2. Mounting plate; 3. Main Power Module Fig. 4-11 Power Module Adapter Key to figure 4-11 1. Power Module Adapter; 2. M3X8 Phillips screw 4.3 Barcode Reader Barcode Reader (Fig.4-13), located in the middle lower of the instrument. Precautions: 1. 2. Applicable type: Code 128, Code 39, Codabar and Interleave 25; The label must be the standard label paper, the normal printer paper is unavailable; 50 AVE-764 3. 4. 5. Service Manual The label should be clicked on the head position of the tube (16x100mm), distance 12.5mm from the tube top. (Refer to the “standard tube”); Placing the labeled tube on the tube rack and insure the label side right against the barcode scanner; If the barcode reader runs abnormally, you could turn off the barcode reader in the system, under the condition that do not affect the normal operation, see the details in 3.2 Barcode Setting. Troubleshooting Process: 1. 2. 3. 4. 5. Checking the barcode reader is open or not in system setting, see details in 3.2 Barcode Setting. Checking the connection of the barcode reader cable is correct, stable or no loose, see the connection diagram in 10.1.1 Main Diagram; Check the barcode reader unlocked or not (barcode reader must be unlocked by standard barcode on first use); The serial port tester sends command to check the barcode reader works properly or not, as showed in Fig.4-12, the command is FB 0C 07. If not, the barcode reader may be broken, and replacement required. Fig.4-12 Serial Port Tester Command Screen Replacing method showed in Fig.4-13. 51 AVE-764 Service Manual Fig. 4-13 Barcode Reader Key to figure 4-13 1. M3X10 Hexagon screw; 2. Barcode Reader assembly Fig. 4-14 Barcode Reader Assembly Key to figure 4-14 1. M3X6 Phillips screw; 2. M3 Flat washer; 3. Reader Box; 4. Barcode Reader 4.4 Cooling Fans & Dust Cover There are two cooling fans at the rear of analyzer, both draw air into the instrument; one is built in PC power module, mainly cool the PC power module and left side of the instrument internal space, another fan is to cool the both sides of instrument internal space. Both of two cooling fans are powered by PC power module with DC +12V. The cooling fans are all equipped with dust covers to prevent the dust entering the 52 AVE-764 Service Manual instrument. CAUTION: To keep good ventilation, suggest to clean the dust covers once a month. The removing of the dust covers showed as Fig.4-15. Washing the removed covers with water, and put it back after drying. If the running speed of the fans is too low, or the fans no turn in the power-good condition, the replacement will be required. See Fig.4-16. Fig. 4-15 Dust Cover Fig. 4-16 Cooling Fan Assembly Key to figure 4-9 1. Type-I M4 nuts; 2. Φ4 ring; 3.Cooling Fan; 4. Guard grill; 5. M4X35 Phillips screw 53 AVE-764 Service Manual 4.5 COM Board The COM board is at the rear of the main unit, it provides two ports –COM7(DB9), Backup COM port; COM8(DB15), Bridge connection port. COM7 is a standard RS-232 serial port, and COM8 is used to connect urine analyzer or bridge(Uritrail etc). Fig. 4-17 COM Board Assembly 54 AVE-764 Service Manual 5 AUTOMATIC SAMPLER 5.1 Summary Automatic sampler, located on the front of the AVE-764, it communication with host computer through serial port; automatic sampler can transfer automatically the sample racks from the ready area on the right side to the pipetting station and then transport the racks to the tested area on the left after sampling. Sample racks, each capable of holding 10 tubes, are loaded on the right side of the sampler. It will transfer the first tube on the rack to the pipetting station if the operator presses the “Start microscopy” button on the main screen after start-up. The test tube location moves after sampling in turn and the sampler will transport the rack to the tested area after all the samples are taken. This module contains several photoelectric sensors in it for detecting tube and rack and positioning motor. Autoloader also has the STAT function as shown in Fig.5-1. In the middle of it located an STAT module. When there is an STAT sample to be tested, just place the corresponding tube in the STAT hole and push it to the front and a prompt dialogue box will pop up from the upper computer. Enter the corresponding information according to the prompt and the instrument will run the STAT test automatically. Fig. 5-1 Front view of automatic sampler 5.2 Component 5.2.1 Function areas The automatic sampler is divided into four functional areas: 55 AVE-764 Service Manual Fig. 5-2 Function area diagram of automatic sampler 5.2.2 Electrical control system The automatic sampler mainly consists of the follow electrical components: 1. Main control board: Control module is the micro processing center of the module, responsible for the processing of every I/O signal and functions as the control center of information exchange with the host computer. 2. Reflective photoelectric sensor: There are lacated at different position for the running track of rack, It is used for the detection of sample racks or sample tube photoelectric sensor. There are all together 7 sensors in this module, and the serial number are: JR1、JR2、JR3、JR4、JR5、JR6、JR8. The functions are listed below: 3. Stepping motor: Stepping motor is the actuator for transporting sample racks, There are 4 motors in this module, and the serial number are: MJ1、MJ2、MJ3、MJ4. The 4. 5. 6. functions are listed below: Inching photoelectric sensor: It is used for locating the original position of the motor. There are 4 sensors in this module. and the serial number are:JS1, JS2, JS3, JS4. The functions are listed below: Correlation photoelectric sensor: It is used for judging whether there is any STAT sample or whether the sample rack has been imported. There are 2 sensor in this module, and the serial number are: JF1, JF2. The functions are listed below: Power switch: The power supply switch of the automatic sampler. 5.2.3 Electric layout The Main control board (03A) connected with electric components of the automatic sample as shown below. 56 AVE-764 Service Manual Fig. 5-3 Main control board (03A) of Automatic Sampler The inner electric layout of the automatic sampler is shown below. Fig. 5-4 Electrical components layout 1 Key to figure 5-4 No. Name Connected to Main control 1 Power switch 2 STAT detection photoelectric sensor 3 Rack in position photoelectric sensor board:J1 Main control board:JF2 Main control board:JR1 57 Function The power supply switch of the automatic sampler. Detect test tube at the STAT position Detect whether the tube rack has been advanced in place when bridged with urine AVE-764 Service Manual analyzer 4 Rack full detection photoelectric sensor Main control board:JR4 Detecting and alarming when the tested area is full of tube racks Fig. 5-5 Electrical components layout 2 Key to figure 5-5 No. Name 5 Tube-detection photoelectric sensor Connected to Function Main control Detect whether there is some sample tube in rack in the position board:JR8 Main control 6 Push-out position photoelectric sensor Main control 7 Tested area rack-detection photoelectric sensor Main control 8 Ready area rack-detection photoelectric sensor 9 Push-in position photoelectric sensor board:JR6 board:JR5 board:JR2 Main control board:JR3 58 Detect whether the sample rack in test area has been transferred to withdrawing position Coordinate with JR4 to detect whether the rack in tested area Coordinate with JR1 to detect whether the rack in ready area. Determine whether the tube rack has been advanced to the test area AVE-764 Service Manual Fig. 5-6 Electrical components layout 3 Key to figure 5-6 No. 10 Name Connected to Main control Push-out zero detection photoelectric sensor 11 Sample-changing terminal detection photoelectric sensor 12 Bridge area motor 13 Bridge area rack-detection photoelectric board:JS2 Main control board:JS1 Main control 14 board:MJ4 Main control board:JF1 Main control board / Main control 15 Push-out motor board:MJ3 59 Function detect the origin position of the mechanical parts of the pushing-out sample rack in tested area detect the terminal of the rack lateral-moving in test area drive mechanical parts in bridge area, transfer rack to ready area detect whether sample rack move into bridge area the micro processing center of automatic sampler Drive mechanical parts in tested area and push the tested sample rack to tested area AVE-764 Service Manual Fig. 5-7 Electrical components layout 3 Key to figure 5-7 No. Name 16 Bridge area zero detection photoelectric sensor 17 Push-in zero detection photoelectric sensor Connected to Function Main control detect the origin position of the mechanical parts of the bridge board:JS4 Main control board:JS3 Main control 18 Sample-changing motor board:MJ2 Main control 19 Push-in motor board:MJ1 detect the origin position of the mechanical parts of the propulsion sample rack in ready area Drive mechanical parts in testing area and move test tube rack laterally to test sample one by one Drive mechanical parts in ready area and push the test sample rack to testing area 5.3 Adjustment 5.3.1 Mechanical testing Power off the device, manual push and pull each motion mechanism, every parts must move smoothly, the slider without locking phenomenon and the pawl turnover normally; Adjust the tension of motion mechanism belt, not too tight or too loose; smooth movement when push and pull the slider; 5.3.2 Reflective photoelectric sensor adjustment Due to the functional difference, so each photoelectric sensor has difference requirement 60 AVE-764 Service Manual for the detection sensitivity. Every photoelectric sensor has sensitivity adjustment potentiometer; you can adjust sensitivity through potentiometer. CAUTION: Sensitivity adjustment: facing the potentiometer, Reduce sensitivity when adjust it clockwise, Increased sensitivity when adjust it anticlockwise. As shown in 5-8, Disconnect the automatic sampler with instrument, take the cover away, then you can adjust the sensitivity of the photoelectric sensor; Fig. 5-8 removing the cover of sampler You need confirm the sensitivity strength by the serial command when you adjust the potentiometer. The description of serial command and corresponding reply is as below. Before use the command to detect the photoelectric sensor or motor, the automatic sampler must power on and communication with host computer normally, they communication through RS-232 serial port protocol, the default port is COM4. The detection of photoelectric sensor need use the SSCOM32 tool. As shown in 5-9. CAUTION: You must quit system software when using the SSCOM32 tool. Configuration of sampler COM ports: Baud rate: 9600 Data bits: 8 Stop bit: 1 Parity bit: none Flow control: none 61 AVE-764 Service Manual Fig. 5-9 SSCOM32 Choose COM4 to set the communication parameters in SSCOM 32. Input test command into string input box, click HexData, then click send, check the hardware motion and the reply information from SSCOM32, judge the status of each parts of sampler. Functionality Command Response Automatically sampler reset FB 08 03 Run reset action after receive the command, return reset successful information as FB 08 03 when everything ok. If communication seems abnormal, check the connecting communication line which connect automatically sampler and host computer has reliable connection. Check firmware version FB 01 FC Return version number when system receives this command. FB E2 00 Put the tube rack near JR1, then the buzzer sounding, and gradually move the tube rack away from JR1, when the distance is greater than or equal to the width of a tube rack, the buzzer is no longer tweet; FB E1 00 If no tube rack stand in ready area, then the buzzer sounding, if a tube rack in ready area, the buzzer is no longer tweet; FB E3 00 Put the tube rack near JR3, then the buzzer sounding, and gradually move the tube rack away from JR3, when the distance is greater than or equal to the width of a tube rack, the Rack in position photoelectric sensor JR1 Ready area rack-detection photoelectric sensor JR2 Push-in position photoelectric sensor JR3 62 AVE-764 Functionality Service Manual Command Response buzzer is no longer tweet; Tube-detection photoelectric sensor JR8 Push-out position photoelectric sensor JR6 Tested area rack-detection photoelectric sensor JR5 Rack full detection photoelectric sensor JR4 Keep this photoelectric sensor on the perfect position where the sensor should facing the tube center, otherwise, adjust photoelectric sensor installation position; and send command at this condition, the hardware should response as below: FB E4 00 Move a tube rack, if a tube(no matter what color of liquid inside or empty) in front of JR8, then the buzzer sounding; if no tube in that position, then the buzzer is no longer tweet; Attention the detection sensitivity of this photoelectric sensor, Prevent undetected and false detection. FB E5 00 Put the tube rack near JR6, then the buzzer sounding, and gradually move the tube rack away from JR6, when the distance is greater than or equal to the width of a tube rack, the buzzer is no longer tweet; FB F3 00 If no tube rack stand in tested area, then the buzzer sounding, if a tube rack in tested area, the buzzer is no longer tweet; FB F2 00 Put the tube rack near JR4, then the buzzer sounding, and gradually move the tube rack away from JR4, when the distance is greater than or equal to the width of a tube rack, the buzzer is no longer tweet; 5.3.3 Motor testing As the same of the photoelectric sensor testing, the four motors also can use this way to test whether is normal or not; Choose COM4 to set the communication parameters in SSCOM 32. Input test command into string input box, click HexData, then click send, check the hardware motion and the reply information from SSCOM32, judge the status of each parts of sampler. Functionality Command Response Push-in motor test FB E7 E2 Push-in motor push the tube rack advancing in position and then back to origin position (if no tube rack in ready area, the motor does not operate), if the error occurred during the process of movement, then the buzzer sounding. Sample-changing FB E8 E3 If the motor at origin position, then it should 63 AVE-764 Functionality Service Manual Command Response motor test Push-out motor test Bridge area motor test move to terminal; if the motor not at origin position, then it should back to origin position, if the error occurred during the process of movement, then the buzzer sounding. FB E9 E4 Push a tube rack out, then the motor back to origin position, if the error occurred during the process of movement, then the buzzer sounding. FB F1 EC If the motor at origin position, then it should move to terminal; if the motor not at origin position, then it should back to origin position, if the error occurred during the process of movement, then the buzzer sounding. Motor troubleshooting: During the test, if the motor is abnormal, it should investigation as below: 1. Check the main control broad power supply; 2. Check the motor connection line, confirm the poor contact; 3. Check the motor belt, make sure the tightness is ok; 4. Check the photoelectric sensor which together with motor; refer 5.35; 5. Change Main control board; 6. Change breakdown motor; 5.3.4 Correlation photoelectric sensor testing The automatic sampler has 2 Correlation photoelectric sensors, one is JF2(as shown in 4-4) which in STAT position, other one is JF1(as shown in 4-6) which in bridge area use for detecting whether sample rack move into bridge area. We can determine whether the correlation photoelectric sensor is normal or not by using the multimeter measure their voltage. Power on the automatic sampler, when the groove of Correlation photoelectric sensor have been sheltered , use the multimeter measure the 1,2 pin, the voltage should less than 1V, and measure 1,3 pin, the voltage should be 5V; when no shelter for the correlation photoelectric sensor, use the multimeter measure the 1,2 pin, the voltage should greater than 4V, and measure the 1,3 pin, the voltage should be 5V; otherwise, you need change the correlation photoelectric sensor or check the connection line between sensor and Main control board. 5.3.5 Inching photoelectric sensor testing It is used for locating the original position of the motor. We can determine whether the inching photoelectric sensor is normal or not by multimeter test it. Power off the automatic sampler, Depress the Spring leaf of this sensor, when the digital multimeter measure the 3 pin of the sensor and 1 pin of com port, the multimeter should display ”0”on its screen and sounding ; Loosen the Spring leaf of this sensor, when the 64 AVE-764 Service Manual digital multimeter measure the 3 pin of the sensor and 1 pin of com port, the multimeter screen should display”--”and no sounding. The pins of the inching photoelectric sensor are shown in 5-12. 5.4 Maintenance 5.4.1 Daily maintenance of automatic sampler To make sure the sampler running properly, timely cleaning the sampler is highly recommended when there are liquid leaked on the sampler. CAUTION: Immediately power off the sampler and clean it while a whole rack samples are pouring onto the sampler. Tools required: Disinfectant/detergent, gauze, and protective gloves. Procedure: 1. Power off the instrument. Ensure that the maintenance is performed under power cut-off condition. 2. Moisten gauze with disinfectant/cleanser and wipe the sampler to remove any deposits. 3. Check the flexibility of pawls. Moisten cotton swabs with disinfectant/cleanser to clean pawls, remove any deposits. 4. Wipe again using distilled water. 5. Power on the instrument until the sampler is totally dry. 5.4.2 Reflective photoelectric sensor maintenance Suggest to clear and maintenance the reflective photoelectric sensor monthly. Maintenance object: 7 reflective photoelectric sensors and Serial number as: JR1, JR2, JR3, JR4, JR5, JR6, and JR8 as shown in fig. 5-5, 5-6. Tools required: Disinfectant/detergent, cotton swabs, protective glove Maintenance Procedures: 1. Power off the instrument, make sure the operation is in shutdown state. 2. Using a cotton swab moistened with absolute alcohol to swab the surface of photoelectric sensors 3. Swabs dry them by using a dry cotton swab. 65 AVE-764 Service Manual 5.5 Replacement 5.5.1 Reflective photoelectric sensor replacement Fig. 5-10 reflective photoelectric sensor replacement Replacement procedure (JR1, JR2, JR3, JR4, JR5, JR6, JR8): 1. Power off the instrument and automatic sampler. 2. Disconnect the terminal between the automatic sampler and host computer, remove sampler away from instrument. 3. Remove the face shield away from the automatic sampler. 4. Find the corresponding photoelectric sensor, disconnect the terminal of it. 5. Loose two screws on PCB. 6. Replace the photoelectric sensor. 7. Reverse the above steps and install photoelectric sensor back. 8. Test the new sensor and make sure it can work normally. 5.5.2 Correlation photoelectric sensor replacement 66 AVE-764 Service Manual Fig. 5-11 Correlation photoelectric sensor replacement Replacement procedure (JF1, JF2): 1. Power off the instrument and automatic sampler. 2. Disconnect the terminal between the automatic sampler and host computer, remove sampler away from instrument. 3. Remove the face shield away from the automatic sampler. 4. Find the corresponding photoelectric sensor, disconnect the terminal of it. 5. Loose two screws on PCB. 6. Replace the photoelectric sensor. 7. Reverse the above steps and install photoelectric sensor back. 8. Test the new sensor and make sure it can work normally. 5.5.3 Inching photoelectric sensor replacement Fig. 5-12 inching photoelectric sensors replacement Replacement procedure (JS1, JS2, JS3, JS4): 1. Power off the instrument and automatic sampler. 2. Disconnect the terminal between the automatic sampler and host computer, remove sampler away from instrument. 3. Remove the face shield away from the automatic sampler. 67 AVE-764 4. 5. 6. 7. 8. 9. 10. Service Manual Remove the clapboard away from the automatic sampler. Remove waterproof board. Find the corresponding photoelectric sensor, disconnect the terminal of it. Loose screws on inching photoelectric sensor. Replace the inching photoelectric sensor (with connection line). Reverse the above steps and install photoelectric sensor back. Test the new sensor and make sure it can work normally. 5.5.4 Main control board replacement Fig. 5-13 Main control board replacement Replacement procedure (03A): 1. Power off the instrument and automatic sampler. 2. Disconnect the terminal between the automatic sampler and host computer, remove sampler away from instrument. 3. Remove the face shield away from the automatic sampler. 4. Disconnect the terminal of photoelectric sensors (J R1、JR2、JR3、JR4、JR5、JR6、 JR8、JF2). 5. 6. 7. 8. 9. 10. 11. 12. Remove the two pawls. Remove the clapboard. Remove the waterproof board. Disconnect all terminals on Main control board. Remove four copper studs which fixed the Main control board. Replace the Main control board. Reverse the above steps and install Main control board back. Test the new board and make sure it can work normally. 68 AVE-764 Service Manual 5.5.5 Motor replacement Fig. 5-14 Motor replacement Replacement procedure (MJ1, MJ2, MJ3, MJ4): 1. Power off the instrument and automatic sampler. 2. Disconnect the terminal between the automatic sampler and host computer, remove sampler away from instrument. 3. Remove the face shield away from the automatic sampler. 4. Disconnect the terminal of photoelectric sensors (J R1、JR2、JR3、JR4、JR5、JR6、 JR8、JF2). 5. 6. 7. Remove the two pawls. Remove the clapboard. Find the corresponding motor and module which this motor belongs to, remove this module away from automatic sampler. 8. Remove the motor away from module. 9. Replace the motor. 10. Reverse the above steps and install back. 11. Test the new motor and make sure it can work normally. 69 AVE-764 Service Manual 6 PUMP & VALVE SYSTEM MODULE 6.1 Summary Pump & Valve system module’s main function is to take certain amount of sample out of the tube, transfer it into the microscopy field, after sample detection finished then washes the pipeline system, in order to prevent cross-contamination. The main process could divide into followed modules, such as Wash, Rev-wash, Maintain, Spe-wash, Sample, etc. All the process modules are controlled by system processing center, if needed; orders from system will assign a module to work automatically. Fig.6-1 Pump & Valve module pipeline schematic diagram Wash: Use cleanser A to wash sampling pipeline, proceed in two phases: sampling needle inner-wall and out-wall wash. Taking CH 1 as an example, the flow path is as following: 1. Cleanser A comes out of cleanser A package, through connecting pipeline via Valve DZ9, Cleanser detector, Cleanser pump, Valve DZ10(normally open), Sampling needle out-wall into Wash pool, and via DZ1(normally open), Waste pump into Waste container; 2. Cleanser A comes out of Cleanser A package, through connecting pipeline via Valve DZ9, Cleanser detector, Cleanser pump, Valve DZ10(normally close), then via Valve DZ32, Counting chamber, Valve DZ31, Sampling needle inner-wall into Wash pool, and via Valve DZ1(normally open), Waste pump into Waste container. Rev-wash (Reverse-wash): Process comes out the same as normal wash process, in order to make sure it is more clean, to be called enhance wash. 70 AVE-764 Service Manual Maintain: The analyzer needs maintenance after daily operation. Click Quit icon on Microscopy interface and select Quit and Maintenance in the pop up dialog to run maintenance wash automatically. The maintenance wash is similar with reverse wash in flow path, only except using maintenance cleanser and open Valve DZ8 not DZ9. Spe-wash (Special-wash): If stains and spots are found in Counting chamber, select Spe-wash, the process is similar to Rev-wash only except using Cleanser B and open Valve DZ7 not Valve DZ9. Sample: During the Sampling operation, sampling needle will be pulled out of wash pool, get into the sample tube, then the Valve of selected channel will be open automatically(such as Valve DZ31 or DZ32), waste pump works, pump in and out three times in order to make sure the sample is fully mixed, sample in and transfer into Counting chamber via selected pipeline. 6.2 Components Pump & valve system is consist of Main control board, Extend controller module components, Triple valve and wash pool. Extend controller module components are consist of the three valves on the right-side of Counting chamber and Adapter Board, etc. 6.2.1 Main control board (02A) The Main control board located on the left side cross-plate (front-side view), open the left side-plate, pull off all the plugs and unlock all the fixing screws to take off the board and replace. After replacement, put back all the plugs and screws in reverse, make sure all plugs and sockets are one to one correspondence and in right direction. CAUTION: After replacement, users must log in system as service level, select Restore Factory Parameter in the drop-down menu of Management to rewrite the former parameter into the new Main control board. Fig.6-2 Main control board Key to figure 4-8 1. 3*6 cross pan-head screw; 71 AVE-764 Service Manual 2. Main control board 6.2.2 Extend Controller Module Components The structure of Extend controller module Components shows below. Fig.6-3 Extend controller module components (Positive axonometric views) Fig.6-4 Extend controller module components (Opposite axonometric views) Key to figure 6-3, 6-4 No. 1 2 3 4 Name DZ9 DZ8 DZ7 Cleanser Detector Connect to Extend control board: 02B-DZ9 Extend control board: 02B-DZ8 Extend control board: 02B-DZ7 Extend control board: 02B-DZ32 5 Cleanser Pump Extend control board: 02B-MJ1 6 Waste pump Extend control board: 02B-MJ2 72 Function Cleanser A valve Maintenance cleanser valve Cleanser B valve Detect the existence of Cleanser A. The motor drives peristaltic pump rotating to squeeze the pipeline and pull the cleanser into the pipeline. The motor drives peristaltic pump rotating to squeeze the AVE-764 Service Manual No. Name Connect to 7 8 9 10 DZ2 DZ32 DZ42 DZ52 Extend control board: 02B-DZ2 Extend control board: 02B-DZ32 Extend control board: 02B-DZ42 Extend control board: 02B-DZ52 11 Bidirectional valve 10 Extend control board: 02B-DZ10 12 Bidirectional valve 1 Extend control board: 02B-DZ1 13 Extend control board Main control board: 02A-J7 Function pipeline to drain out the waste after cleansing. Waste valve Left valve of Channel 1 Left valve of Channel 2 Left valve of Channel 3 Normally open or closed to change the trend of waste in pipeline. Normally open or closed to change the trend of cleanser in pipeline. Control module of pump motors, valves, the interface adapter for cleanser detector 6.2.3 Triple Valve Components The structure of Triple valve components is as shown below. Fig.6-5 Triple Valve Components Key to figure 6-5 No. 14 15 16 17 Name Adapter Board DZ31 DZ41 DZ51 Connect to Main control board: 02E-DZ31 Adapter Board:02E-DZ31 Adapter Board:02E-DZ41 Adapter Board:02E-DZ51 Function Right valve of Channel 1 Right valve of Channel 1 Right valve of Channel 2 Right valve of Channel 3 6.2.4 Wash pool The Wash pool as shown in Fig.6-6, located at mid-lower part of the instrument, which is a hollow container with a drain hole at the bottom and cleansing pipeline on top used for cleansing sampling needle wall. There are two pairs of electrode used for detection of 73 AVE-764 Service Manual high and low level inside of wash pool. Wash pool is not only used for collecting tested sample but also cleansing waste, sampling needle out-wall cleansing waste, etc. The waste flows to waste container through the Wash pool eventually. Fig.6-6 Wash pool Key to figure 6-5 1. 3*10 cross countersunk head screw; 2. Wash pool 6.3 Adjustment Login software, enter the Management—Adjustment interface. In Adjustment interface, users are able to adjust the parameters of pump & valve components, as shown in Fig.6-7. 74 AVE-764 Service Manual Fig.6-7 Adjustment interface 6.3.1 Pump & valve testing 1. Click Valve button in Adjustment interface to enter into Pump & Valve Test interface, users can test all the valves separately by ticking On/Off options here. Fig.6-8 Pump & Valve Test interface 2. If it’s needed to test single pump & valve components, select the corresponding On/Off options to operate, click Start and observe whether the component works correctly or not. If it’s not working correctly, the reason may be broken or failure in the controlling circuit. Try to locate where is the problem by using contiguous same-kind components to test. Troubleshooting Operations (take Valve DZ8 as example): 1. If DZ8 is not working, exchange the plug of contiguous working component (such as DZ9) to the socket of DZ8, then select On/off options to test and observe. If it’s working again after exchange, that means the Valve DZ8 is broken and needed to be replaced; If it’s still not working, that means the adapter board may have circuit failure, replace a new adapter board(02B), bring back all the plugs and connectors and observe; If it’s still not working, try to replace the main controlling board(02A) 75 AVE-764 Service Manual according to 6.2.1 Main control board (02A). CAUTION: Because Valve CH1, Valve CH2, Valve CH3 controls both left and right side valves of counting chamber, once users select the On/Off options, valves on both sides will respond at the same time. 6.3.2 Pump-Valve Calibration CAUTION: Pump-Valve calibration is recommended at following conditions: 1. Bubbles occur in counting chamber or the amount of reagent is abnormal; 2. After replacement of rinse/waste bath or tubes (including pump-line, valve-line, etc); 3. Monthly maintenance; Operate Pump-Valve calibration as following (CH1 as example): 1. Sample-pump Calib: By selecting SPC (1#, 2# and 3# respectively), a dialog box will pop up as shown in Fig.6-9. Click OK to continue, the new value will be saved automatically. The reference value for Sample-pump Calib is 4-5.5s, repeat sample pump calibration till the value meets the reference range. 2. Fig.6-9 Sample-pump Calib pop-up interface Wash-pump Calib: By selecting WPC (1#, 2# and 3# respectively), a dialog box will pop up as shown in Fig.6-10. Click OK to continue, the new value will be saved automatically. The reference value for Wash-pump Calib is 3.5-5s, repeat wash pump calibration till the value meets the reference range. Fig.6-10 Washing-pump Calib pop-up interface After calibration, operate as following to confirm: Wash-pump Calib confirmation 1. Use measuring cylinder to prepare 10 ml cleanser A, put the pipeline connect to cleanser A container into the cylinder; 2. Click Wash button 3 times in Adjustment interface as shown in Fig.6-7, observe whether there is 1ml cleanser A left or not, if yes, it is confirmed that the instrument use 3ml cleanser A each time, error limit of the reagent consumption ≤0.4ml. 3. If the reagent consumption>3ml each time, please find “IniConfig.ini” in the installation directory of D:\AVE, open the file and find “VentPumpZhi” under “[CommSet]”, reduce the number a little bit; Otherwise, rise the number a little bit. Click Save button to confirm the changes. “VentPumpZhi” range is : 3800~4800; 4. Enter the Adjustment interface, click Pump button to redo, after finished save and 76 AVE-764 Service Manual Quit. 5. Repeat Step 1~2 to confirm the reagent consumption is 3ml or not, if not, repeat Step 3~4. Sample-pump Calib confirmation 1. Prepare 3 measuring tubes filled with 10 ml saline, put them into a rack, transfer the rack to check-waiting field; 2. Click Samp All button 4 times in Adjustment interface as shown in Fig.6-7, observe whether there is 4ml left in each tube or not, if yes, it is confirmed that the sample consumption volume is 1.5ml each time, error limit of sample consumption ≤0.4ml. 3. If the sample consumption>1.5ml each time, please find “IniConfig.ini” in the installation directory of D:\AVE, open the file and find “SuckPumpZhi” under “[CommSet]”, reduce the number a little bit; Otherwise, rise the number a little bit. Click Save button to confirm the changes. “SuckPumpZhi” range is : 3500~4500; 4. Enter the Adjustment interface, click Pump button to redo, after finished save and Quit. 5. Repeat Step 1~2 to confirm the sample consumption is 1.5ml or not, if not, repeat Step 3~4. Pump-Valve calibration can also be done in another way: Enter Microscopy main interface as shown in Fig.6-11, click Tools button and select Calibration Functions in the drop-down menu, system will pop up Calibration Functions interface as shown in Fig.6-12. This is also for professional use only. Fig.6-11 Calibration Functions in Microscopy screen Fig.6-12 Calibration Functions interface 77 AVE-764 Service Manual 6.3.3 Blank Calibration It is used to determine dirty spots number in the Counting Chamber and improve the precision of the instrument. Operations can be done on the right side of the Calibration interface, in the “Blank Calib” field as shown in Fig.6-12. 1. Prepare 3 tubes filled with a blank reagent (0.9% NaCl). 2. Click the Blank button. Blank Calibration will be done automatically. 3. A prompt will appear after finished. 4. Click Save to confirm the certain number of dirty spots. It will be subtracted from each result randomly. It is recommended using the blank calibration to determine whether the channels are clean or not. If the dirty spot exceed 5, the channels might need additional cleaning or replacement. CAUTION: If the spots number>5, please do Spe-wash and Blank Calibration again until it meets the range; Otherwise, it’s recommended to replace the Counting Chamber. 6.4 Maintenance and servicing 6.4.1 Quit and Maintain CAUTION: After daily operations, it’s strongly recommended to select Quit and maintain when exit, the instrument needs daily maintenance and cleansing in case failures in pipeline system. Fig.6-13 Quit dialog interface 6.4.2 Valve-tube Maintenance CAUTION: Cause the valve-tube is clamped when the valve is power off, in case the valve-tube failures or blocking, it’s recommended to maintain regularly. Operations: 1. Take the valve-tube out of valve; squeeze the deformation spot to bring it back to shape; 2. It’s recommended to move the valve-tube up or down 2mm regularly in order to prevent the same spot be clamped too long and observe whether it is broken or not. 3. If the valve-tube is broken, blocked, can’t be cleansing, can’t bring back to shape, please replace a new one in the same size and specification. Valve-tube Specifications: Name Material 78 Spec(inner/outer diameter) AVE-764 Service Manual Single channel valve-tube Silicon Φ2mm×Φ4mm Dual channel valve-tube Silicon Φ1.5mm×Φ3mm 6.4.3 Other pipelines Maintenance CAUTION: The maintenance of other tubes is recommended at following conditions: Operations: 1. Tubes are blocked urine scale or other substance; 2. The connecting spot is ageing, losing stretch, leaking, etc. 3. If maintenance of the tubes can’t solve the problem, please replace a new one in the same size and specification. Other tubes Specifications: Name Material Spec(inner/outer diameter) Tubes at both ends of wash pool Silicon Φ1mm×Φ3mm Waste tube(outer) PU Φ2.5mm×Φ4mm Tube(from the bottom of rinse-bath to Dual-clip valves) Teflon Φ2mm×Φ3.2mm Other tubes Silicon Φ2mm×Φ4mm 6.4.4 Counting Chamber Maintenance CAUTION: If the instrument can’t suck in sample liquid or bubbles appear in counting chamber, it may cause by channel blocking, please operate as following: Operations: 1. Enter Adjustment interface, select corresponding channel, click Spe-wash button and observe can it be cleansed or not, if can’t, please continue Step 2; 2. Take off the counting chamber, connect one end to a injector and put the other end into cleanser (diluted hydrochloric acid, 84 Disinfectant, detergent solution, etc) container, repeat suction and pumping certain times, until it’s cleansed; 3. After all, if the counting chamber is till not able to be cleansed, please replace a new one, see 8.5.2 Replacement of Counting Chamber for details. 6.4.5 Wash pool Maintenance CAUTION: It’s recommended that washing and cleansing the wash pool monthly, mainly including wash pool inner-wall, cleanser entrance and waste exit, in order to prevent the electrode out of function or tube blocking. Tools required: deionized water, cotton swab, tube brush, safety gloves; Operation Steps: 1. Make sure the instrument is powered off; 2. Open the front mask, locate the counting chamber, rise the sampling needle; 3. Use tube brush dipped in deionized water to clean wash pool inner-wall; 4. Use cotton swab dipped in deionized water to clean cleanser entrance and waste exit; 79 AVE-764 5. 6. Service Manual Put back the sample needle and fold the front mask; Restart the instrument, log in the system software, enter the Adjustment interface, click Wash All button 1~2 times to confirm. 6.5 Replacement 6.5.1 Peristaltic-pump tube replacement CAUTION: Peristaltic-pump tube is a consumable item, it’s recommended to check and maintain regularly, normally every 6 months. If the tube is ageing, losing stretch or broken, please replace a new one ASAP. CAUTION: Peristaltic-pump tube specification: Φ3.2 (inner diameter)*Φ6.4 (outer diameter)*92 (length) mm, make sure the replacement tube is in the same size and specification. Biological Warnings: Before replacement, please follow the safety operation specification and wear personal protective equipment (such as safety clothing, safety gloves, etc). Fig.6-14 Side view of Peristaltic Pump Replacement Steps: 1. As shown in Fig.6-15, open the pump cover anticlockwise; 80 AVE-764 Service Manual Fig.6-15 Open the pump cover 2. Fig.6-16 Side view of pump (without cover) Take out both ends of the pump tube from the groove, as shown in Fig.6-17. 81 AVE-764 Service Manual 3. Fig.6-17 Take out both end of the pump tube Take off the pump tube , as shown in Fig.6-18 4. Fig.6-18 Take out the pump tube Replace a new pump tube and connect both ends tight and correctly. 82 AVE-764 Service Manual 5. Fig.6-19 Replace a new pump tube Bring the new pump tube back and fix both ends to the groove. 6. Fig.6-20 Bring backs the pump tube and fix the ends Put back the pump cover. 83 AVE-764 Service Manual Fig.6-21 Put back the cover CAUTION: In order to ensure the accuracy of sampling and wash consumption, please redo pump calibration operation, see 6.3.2 Pump-Valve Calibration for details. 6.5.2 Disassembly diagram of the pumps and valves It’s not necessary to break up the entire pump-valve bracket in most cases. Open the left and rear panels for maintenance, if it’s needed, unplug the connecting cable between the Pump & Valve system module from the chassis first and unplug other pipeline joints at the same time, then tear down certain components. Fig.6-22 Disassembly of the Pump & Valve system module Disassemble Bidirectional valve assembly 84 AVE-764 1. 2. Service Manual Tear bidirectional valves down from the bracket as shown in Fig. 6-23. Unplug the JZ1 and JZ10 first, and open the wire slot along the cable trend, cut the cable-ties, take off the entire component. Fig.6-23 Disassembly of the bidirectional valve Next, tear apart the bidirectional valves as shown in Fig. 6-24. Fig. 6-24 Disassembly of the bidirectional valves Key to figure 6-5 1. M3*10 Cross pan-head screw 2. M3 Spring washer 3. Bracket 4. Bidirectional valves 3. Pay attention to the different directions and don’t press the cable when assembling. Assemble it in reverse. Disassemble One-way valve assembly 1. Tear apart the one-way valve assembly 2 from the bracket as shown in Fig.6-25. Unplug the DZ2, DZ32, DZ42 and DZ52 first, and open the wire slot along the cable trend, cut the cable-ties, take off the entire component. 85 AVE-764 Service Manual Fig.6-25 Disassembly of one-way valve assembly 2 Key to figure 6-5 1. M3*10 Cross pan-head screw 2. Bracket 3. One-way valve 2. Next, tear apart the one-way valves as shown in Fig. 6-26. Fig.6-26 Disassembly of one-way valves Key to figure 6-5 1. One-way valve 2. Bracket 3. M3*6 Combination pan-head screws Disassemble One-way diaphragm valve assembly It’s not necessary to break up the entire entire one-way diaphragm valve assembly from the Pump & Valve system module. Remove the two M3*6 combination pan-head screws, unplug the connecting cable. open the wire slot along the cable trend, cut the cable-ties, take off the entire component as shown in Fig. 6-27. 86 AVE-764 Service Manual Fig. 6-27 Disassembly of one-way diaphragm valves Key to figure 6-5 1. One-way diaphragm valve 2. M3*6 combination countersunk-head screws Disassemble Pumps Fig. 6-28 Disassembly of pumps Key to figure 6-5 1. Pumps 2. M3*10 Combination screws 3. Bracket Disassemble Extend control board (02B) 1. Unplug all the connecting cables and remove all the fixing screws as shown in Fig.6-29. Tear apart all 4 brass stud as shown in Fig.6-30. 87 AVE-764 Service Manual Fig.6-29 Remove all the fixing screws 2. Fig.6-30 Take off all 4 brass stud and the Extend control board As shown in Fig.6-29,6-30, take off the Extend control board, replace a new one and put it back in reverse. 88 AVE-764 Service Manual 6.5.3 Triple valve replacement Fig.6-31 Adapter Board Key to figure 6-5 1. M3*6 Cross pan-head screw 2. Adapter Board 1. As shown in Fig.6-31, if it’s needed to replace the adapter board, unplug all the connecting cables, remove all the fixing screws, replace a new board and put back the cables and screws correspondingly. Fig. 6-32 Disassembly of the Triple valve Key to figure 6-5 1. Bracket 2. Valve 3. 3*M6 Cross pan-head screw 2. As shown in Fig.6-32, remove the 4 fixing screws, bring down the entire bracket. 89 AVE-764 Service Manual Fig. 6-33 disassembly of the one-way valve Key to figure 6-5 1. Valve 2. Bracket 3. M3*6 Cross Combination screws 3. The disassembly process of the Triple valve must be in order: first, tear apart the entire bracket, and then tear down the valve off separately. 90 AVE-764 Service Manual 7 SAMPLING MODULE 7.1 Summary The Sampling module is located in front of the instrument; it consists of sampling control board (02C), sampling pinboard (02D), sampling needle motor and linear guide rail. When the Sampling module receive the instruction from the analysis processor, the sampling needle will take in a certain amount of sample after mixed from the test tube, and transfer it to the field of the microscope. This Sampling module has function of conductivity detection, anti-collision and liquid level detection. 7.2 Components The Components of the Sampling module are as shown in 7-1. Fig. 7-1 Sampling module The electric component of Sampling module as below: Key to figure 7-1 NO. Name Connect to Function 1 Sampling control board 02C Pump valve main control board: 02A-J12 Sampling motor drive circuit 2 FFC flat cable Sampling control board: 02C-J6 The connecting cable of 91 AVE-764 NO. Service Manual Name Connect to Function Sampling control board: 02D-J7 control board and pinboard. Sampling control board:02C-J6 The interface adapter of Sampling arm 3 Sampling pinboard 02D 4 Up-down photoelectric sensor Sampling control board: 02D-J3 Locate the origin position when the sampling arm moves up and down 5 Up-down motor Sampling control board: 02D-J2 Drive the Sampling arm to move up and down 6 Sampling needle photoelectric sensor Sampling control board: 02D-J1 Locate the origin position of the sampling needle 7 Back-forth motor Sampling control board: 02C-J7 Drive the sampling arm to move back and forth 8 Back-forth photoelectric sensor Sampling control board: 02C-J8 Locate the origin position when the sampling arm moves back and forth Sampling control board: 02D-J6 Draw in the sample from the tube and has functions of anti collision, conductivity detection and liquid level detection. 9 Sampling needle 7.3 Adjustment 1. Login the software, enter Management-Adjustment, click ↓ button beside Sample button, enter Sampling module setting interface, as shown below. Fig. 7-2 Sampling module adjustment interface 2. Prepare 3 tubes of physiological saline, placed in tube rack. 3. Load the tube rack on ready area, click Sample button, observe the position of the sampling needle, and go to step 4 to adjust the needle according the criterion below. Criterion for the Sampling module adjustment 92 AVE-764 Service Manual Project Features Criterion Pool->Sample The distance of sampling needle from wash pool to normal sampling position Sampling needle should be at the center of the tube Pool->STAT The distance of sampling needle from wash pool to SATA position Sampling needle should be at the center of SATA position Bottom of tube The position of sampling needle insert into the tube Sampling needle distance 5mm from bottom of the tube Bottom of pool The position of sampling needle insert into wash pool Sampling needle distance 1mm from bottom of the wash pool High level position Sampling needle stopping detect liquid level position The tip of Sampling needle is away from nozzle of tube 4cm F-B motor overshoot The overshoot when the F-B motor reachs to zero point Fixed as 3.8mm F-B motor speed The speed of the F-B motor Fixed as 2 U-D motor overshoot The overshoot when the U-D motor reachs zero point Fixed as 3.8mm U-D motor speed The speed of the U-D motor Fixed as 2 The pump run certain time to drain waste from pipeline internally The time should be sufficient to drain waste The pump run certain time to drain Cleanser A from pipeline internally The time should be sufficient to drain Cleanser A The pump run certain time to drain Cleanser A from washing pool (sampling needle outer) The time should be sufficient to drain Cleanser A The pump run certain time to aspirate Cleanser B to counting chamber The time should be sufficient to aspirate Cleanser B The pump run certain time to drain Cleanser B from pipeline internally The time should be sufficient to drain Cleanser B Drain waste time (inner) Drain Cleanser A time (inner) Drain Cleanser A time (outer) Aspirate Cleansen B time Drain Cleanser B time 4. 5. Based on the actual situation, input the proper parameter into input box which corresponding adjustment item. Click Test button, then the sampling arm will perform operations after receive the instruction, observer its situation and if necessary, repeat adjusting until fulfill the adjustment criterion. Click Save button after adjustment. 93 AVE-764 6. Service Manual If no response from the Sampling module, see 7.5 Replacement to replace it if necessary. 7.4 Maintenance Dredge the sampling needle If the sampling needle is blocked, following the below steps to dredge the sampling needle. 1 Make sure the analyzer is in shutdown state. 2 Open the front cover to access the waste pool. Manually lift the sampling needle out. 3 Remove the pipelines from the top of needle, use the pricker thread through from top of the needle and exit out from the end, clean the dirty stick on the pricker; 4 Repeat step3 several times until there is no dirty stick on. 5 Connect the pipeline back to the needle. Move the needle back to the waste pool and close the front panel. 6 Restart the instrument and login the software, go to Adjustment interface and click Wash All button several times to perform channels washing. 7.5 Replacement CAUTION: Components replacement only can be done in shutdown state. 7.5.1 Replacing sampling control board (02C) Fig. 7-3 Sampling control board Key to figure 7-3 1. M3X6 Screw 2. Sampling control board 1. As shown in Fig. 7-3, unplug the cable plug, and loosen the screws, then replace sampling control board. 94 AVE-764 Service Manual 7.5.2 Replacing sampling pinboard (02D) Fig. 7-4 Sampling pinboard Key to figure 7-4 1. M3X6 Screw 2. Sampling pinboard 3. the fixed board 4. M3X6 Screw 1. As shown in fig. 7-4, loosen the screws on the fixed board first, then loosen the screws on sampling pinboard, and replace the sampling pinboard. 7.5.3 Replacing back-forth photoelectric sensor Fig. 7-5 Back-forth photoelectric sensor 95 AVE-764 Service Manual Key to figure 7-5 1. back-forth photoelectric sensor 2. M2.5X6 Screw 1. As shown in fig. 7-5, loosen the screw, unplug the sensor plug, then replace the back-forth photoelectric sensor. Login the software; see 7.3 Adjustment to adjust the sampling needle to proper position. 7.5.4 Replacing up-down photoelectric sensor Fig. 7-6 Up-down photoelectric sensor Key to figure 7-4 1. M2.5X6 Screw 2. up-down photoelectric sensor 1. As shown in 7-6, loosens the screw, unplug the sensor plug, then replace the up-down photoelectric sensor. After the replacement, login the software; see 7.3 Adjustment to adjust the sampling needle to proper position. 96 AVE-764 Service Manual 7.5.5 Replacing motors Fig. 7-7 Motors 1. Power off the instrument. 2. Open the front cover, disconnect the plug all wires connected to Sampling module, and disconnect the pipelines connected to sampling needle. 3. Loosen the 6 fixed screws that fix the Sampling module and remove the complete Sampling module out from the device. 4. Find the corresponding motor, loose the screws as shown in fig. 7-7. 5. Replace the new motor. 6. Install new motors back by repeat the above steps in reverse. 7. See 7.3 Adjustment to adjust the Sampling module to proper situation. 7.5.6 Replacing sampling needle 97 AVE-764 Service Manual Fig. 7-8 Sampling needle module Key to figure 7-8 1. Fixing ring 2. Screw 3. Guide rod 4. Spring 5. Sampling needle 6. Light block 7. Mounting module 8. Fixing ring Fig. 7-9 Sampling needle photoelectric sensor Key to figure 7-9 98 AVE-764 Service Manual 1. Sampling needle photoelectric sensor 2. Screw 3. Sampling needle module 99 AVE-764 Service Manual 8 MICROSCOPE MODULE 8.1 Summary With the aid of automatic controlling system, microscope module of AVE-764 can control the forward/back, left/right, up/down, movements of the microscope stage to ensure the testing items locate in the imaging center; control the up/down movements of condenser to adjust focus; control the auto-switch of HP/LP lens, auto focus of CCD, capturing and photographing of testing items; transfer all the pictures back to the instrument’s main controlling system. System will finish the auto recognition, classification, counting. 8.2 Components Components of microscope module: 1. CCD Camera, controlled by Upper-computer, capture and photograph tested items, transfer the pictures to system; 2. Counting Chamber, CCD will take pictures after sedimentation, the number of channel differs from types, including 1 channel type, 2 channels type, 3 channels type. 3. Microscope main control board, receiving orders from Upper-computer and control all the movement of the microscope; 4. Motors, controlled by Microscope main control board, drive each mechanism; 5. Sensors, locate the initial position of each mechanism. 100 AVE-764 Service Manual Fig. 8-1 Microscope Module (right side view) Key to figure 8-1 1. CCD 2. Monocular 3. Objective 4. Counting Chamber 5. Counting-Chamber bracket 6. Microscope lamp (inside the box) 7. Main body 8. Controlling Board 9. Left-right motor of stage 10. Up-down motor sensor (in the box) 11. Lamp cover fixing screw 12. Adjust knob fixing clamp Fig. 8-2 Microscope Module (left side view) Key to figure 8-2 1. Objective motor 2. Objective motor sensor 3. Up/down motor of stage 4. Left/right motor sensor 5. Focus lamp motor 6. Focus lamp motor sensor 7. Back/forth motor sensor 8. Stage 9. Back/forth motor of stage 101 AVE-764 Service Manual 10. Belt 8.3 Microscope and Image Adjustment Log in the software as service level; select Adjustment-Management to enter the Adjustment interface. Fig. 8-3 Adjustment screen 8.3.1 Preparation for microscope focus adjustment 1. 2. 3. 4. 5. Prepare 3 tubes of RBCs suspensions; put the tubes in a rack. Load the rack onto the ready area of the sampler. Select CH1 in Adjustment interface, click MechRS button to run mechanical reset. Click Samp All button, system will transfer the rack from the ready area to the testing area and run sampling operation separately. Wait for about 2 minutes till the RBCs fully settled in the counting chamber. Until now, the preparation is done and ready to next operation. 8.3.2 Objective Adjustment CAUTION: The rotation parameter of Objective is very important, if users need to modify it, please contact AVE service engineer. 1. Click LP Reset button in the function keys field to ensure the vision-field is under LP; 2. Select Lens in the top-left corner of the interface; 3. Click Reset button below the Step box to reset the objective, after finished, try to rotate the objective, if it’s stuck, that means the objective fit in the “V” groove; if the objective needs to be rotated a little bit to fit in, that means further adjustment is 102 AVE-764 4. 5. 6. Service Manual needed. Further adjustment is that rotate the objective according to hand feel, type in certain steps in the step box, click Reset button and observe, for example, going left click and going right click +. Repeat step 3~4 until the objective fit in the “V” groove. Then click Save LP Mark button. Click HP Reset button in the function keys field, set the HP Lens number to “LP Lens+680” and click Save HP Mark button. CAUTION: Lens parameter won’t differ from CHs, it’s only necessary to set the Lens parameter under CH1 LP/HP, which will automatically apply for CH2, CH3. 8.3.3 Square-Mark Adjustment of Microscope 1. Square-mark is a scale-plate build in each channel of counting chamber, the position of the square-mark determines the initial position of image capturing under LP/HP. Square-mark adjustment reference range under LP/HP as follows: Lens Left-Right F-Backward Up-Down Lens Focus lamp LP 400~800 600~1000 700~1600 50~250 700 HP HP-LP≤120 HP-LP≤120 HP=LP 700~850 200 LP 400~800 CH2-CH1=2830 700~1600 50~250 700 HP HP-LP≤120 HP-LP≤120 HP=LP 700~850 200 LP 400~800 CH2-CH1=2830 700~1600 50~250 700 HP HP-LP≤120 HP-LP≤120 HP=LP 700~850 200 CH CH1 CH2 CH3 2. Factory parameters will be provided with counting chamber, after replacement, enter Management-Adjustment, click Replace chamber button, type in the new parameters provided with new counting chamber, click Save button and Quit. Fig.8-5 Replace chamber window 103 AVE-764 3. 4. 5. 6. 7. Service Manual After parameter modification, the instrument will calibrate the parameters of counting chamber and stage position automatically correspondingly and check whether the square-mark position and focus achieved best condition or not; If not, please adjust microscope according to following operation steps to achieve best state: Select CH 1 in Adjustment interface, click LP Reset button to ensure LP field; Select F-Backward, type in certain steps in the step box(such as 50), click “+” or “-” button to modify the step to 550; Select Left-right, change the step to 400 according to step 5, type in 50 into the step box and click “+” button until the square mark appears; Select F-backward or Left-right, decrease the step typed in the step box orderly (50, 30, 20, 10, 5, etc), only until the square-mark locates as shown in Fig.8-6, then click Save LP Mark button. 8. Fig.8-6 LP square-mark position Click HP Reset button to ensure HP field, repeat step 5~7 to locate HP square-mark as shown in Fig.8-7 and click Save HP Mark button to confirm. 9. Fig.8-7 HP square-mark position Complete square-mark position adjustment for CH2, CH3 by repeat step 4~8, only except the F-Backward step should be 3385(CH2) and 6220(CH3) in step5. 8.3.4 Focus Adjustment After the adjustment of square-mark position, it’s necessary to adjust the focus, operation as follows: Procedure of Auto-Focus: 1. Prepare 3 tubes of RBC suspensions, put the tubes in a rack and load the rack in ready area of sampler; 2. Click Samp All button, wait until finished, select CH1, click Auto-Focus and observe 104 AVE-764 Service Manual HP image, system will complete focus adjustment automatically; 3. Click Save in the pop up window to confirm. 4. Then check the foucus of CH2 and CH3. 5. If the result of Auto-Focus is not desired, continue with Manual-Focus as follows: Procedure of Manual-Focus 1. Select “CH1” then click LP Reset. 2. 3. 4. 5. 6. 7. 8. Check the , and enter 5-10 step in “Step”, click “-” or “+” to adjust the image to the most clear state (compare with the standard gallery). Click “Save LP Mark” to save. Click HP Reset; Check the , and enter 2-5 step in “Step”, click “-” or “+” to adjust the image to the most clear state (compare with the standard gallery). Remember the HP value, then click LP Reset, and enter certain value in “Step” to adjust the LP value same as HP value. Then click “Save LP Mark” to save. Perform the same procedure to calibrate the CH2 and CH3. 8.3.5 Image Adjustment 1. Click Image button in the Adjustment interface as shown in Fig.8-8. Users can set the background brightness, gray level, color gain, etc. Fig.8-8 Image setting window Reference range for parameters: Gray: 12~16, 12 as default; Gain: Green: 15~25; The Soft Gain Picture of Brightness, Red, Green, Blue should be basically the same to Ref value, difference should not be larger than 10; 2. Click Image button in Adjustment interface, select LP in Precise Adjust field; 3. Click AE On and AWB On button one by one, system will adjust automatically. 105 AVE-764 Service Manual 4. 5. Type in Bright/Gray (10) and Gain (1) in Precise Adjust field. Click the L+ or L- button in Brightness field to adjust the image exposure value as same as the reference value. Click Save Exposure to confirm. 6. Click R+/R-, G+/G- and B+/B- buttons to adjust the image gain value to the reference values (difference less than 10). Click Save Gain button to confirm. 7. Click Save All after all finished. Select HP in Precise Adjust field, finish the adjustment in the same way as above. Click Save All button to confirm. 8.4 Maintenance 8.4.1 Cleaning of Counting-chamber and stage Tools required: 95% ethanol, absorbent cotton swab, no-dust cloth; Operation Steps: 1. Turn off the instrument, Open the front mask, use cotton swab dipped in ethanol to clean the surface of chamber; 2. Use cloth dipped in ethanol to clean the stage; 3. Use cotton swabs to wipe until dry; CAUTION: Do NOT wipe forth and backward, please wipe in one direction. 8.4.2 Cleaning of Sensors Tools required: 95% ethanol, absorbent cotton swab; Operation Steps: 1. Turn off the instrument, make sure the power is off; 2. Open the front mask and left side panel; 3. Use cotton swab dipped in ethanol to clean the sensors; 4. Use cotton swabs to wipe until dry; 8.4.3 Cleaning of Micro-lamp and Focus lamp Tools required: 95% ethanol, absorbent cotton swab, no-dust cloth; Operation Steps: 1. Turn off the instrument, make sure the power is off; 2. Open the front mask; 3. Use cloth dipped in ethanol to clean the Focus lamp; 4. Use cloth dipped in ethanol to clean the micro-lamp basement; 5. Use cotton swabs to wipe until dry; CAUTION: Clean the micro-lamp after cooling in case burning. 8.4.4 Maintenance of rotation axis Tools required: 95% ethanol, absorbent cotton swab, lubricating oil; Operation Steps: 1. Turn off the instrument, make sure the power is off; 2. Open the front mask and left side panel; 106 AVE-764 3. 4. Service Manual Use cotton swab dipped in ethanol to clean the rotation axis; Wait till dry, use cotton to lubricate the rotation axis; 8.4.5 Maintenance of counting chamber 1. 2. 3. 4. Maintenance is needed if dirty spot or blocking found in counting chamber, operate as follows. Select corresponding channel in Adjustment interface, click Spe-wash and observer whether it’s cleaned or not, if not, continue Step 3; Take off the counting chamber, connect one end to a injector and put the other end into cleanser(diluted hydrochloric acid, 84 Disinfectant, detergent solution, etc) container, repeat suction and pumping certain times, until it’s cleansed; If still can’t be cleaned, please replace a new one. 8.5 Replacement 8.5.1 Replacement of Micro-lamp Micro-lamp is a consumable item, affected by external environment (such as Voltage, Temperature, Humidity, etc) or using frequency, leading to out of brightness or broken, please replace it based on actual condition. CAUTION: Replacement is recommended when micro-lamp is out/lack of brightness, dark/black images appear, recognition is affected. CAUTION: Specification of micro-lamp: 12V, 20W. CAUTION: Replace the micro-lamp after power off and cooling, replace with one in same size and specification. CAUTION: Replace the micro-lamp after cooling in case burning. Operation steps of replacing the micro-lamp: 1. Power off, make sure the micro-lamp is completely cooled, open the front mask, locate the microscope and the hand-screw on lamp box as shown in Fig.8-9; 2. Unlock the hand-screw, open the micro-lamp box cover, as shown in Fig.8-10; 107 AVE-764 Service Manual Fig.8-9 Hand-screw on micro-lamp box 3. Fig.8-10 Open the micro-lamp box Remove the lamp-shade, as shown in Fig.8-11. 108 AVE-764 4. Service Manual Fig.8-11 Micro-lamp Take off the lamp, replace a new one, put back in reverse; CAUTION: If the new micro-lamp can’t be light, please take off the lam and plug in again, the problem is caused by poor contact randomly. 8.5.2 Replacement of Counting Chamber CAUTION: Counting Chamber is a consumable item, affected by external environment or using frequency, leading to dirty spot, scratches or broken, please replace according to actual condition. CAUTION: If dirty spots or scratcher are in large quantity and can’t be removed, affecting recognition, please replace a new one after power-off and cooling. Operation steps of replacing counting-chamber: 1. Take out the new counting-chamber, double confirm whether the serial number on chamber and the package are the same or not; 2. Open the front mask, locate the counting-chamber, as shown in Fig.8-12; 109 AVE-764 3. 4. Service Manual Fig.8-12 Counting-chamber on the stage Turn the lens a little bit, rise the clips and release the counting-chamber with bracket, disconnect the pipelines and bring out the entire unit, as shown in Fig.8-13; Fig.8-13 Bring out the counting-chamber with bracket Unlock the screws, separate the counting-chamber, as shown in Fig.8-14; 110 AVE-764 5. Service Manual Fig.8-14 separates the counting-chamber and bracket Replace a new chamber, connect the pipelines correspondingly, as shown in Fig.8-15 CAUTION: The installation direction of counting-chamber is that convex-side up and the broadside with serial numbers face inside; The pipeline must connect color to color and cover at lease 1/2 of the connector surface. 6. Fig.8-15 Replace a new counting-chamber Put back the new chamber into bracket after pipelines connected, bring back the screws and fix the new chamber in the bracket, as shown in Fig.8-16. CAUTION: Install the new counting-chamber back into the bracket, upper-right corner against the bracket; The chamber tabletting should press to the 111 AVE-764 Service Manual chamber edge from 1-2 mm. Fig.8-16 Fixing the new chamber in bracket 7. Push the bracket back under the tabletting. Make sure the positioning balls just drop into the fixed holes. 8. Close the front panel after finished. 9. Input the parameters of new counting chamber. After replacing the counting chamber, the parameters must be modified in the software. Log in software, enter Management-Adjustment, click Replace Chamber button to pop up the Replace chamber interface, as shown in Fig.8-17, type in the parameters of new chamber (provide with chamber package box). Click Save button to confirm and Quit button to exit. 10. Focus Confirmation After parameters modification, microscope system will calibrate based on new parameter automatically. Check focus and square-mark position under LP/HP of each channel. If the condition is not satisfied, refer to 8.3 Microscope and Image Adjustment for further operations. 11. Calibration and Verification of chamber coefficient Launch QC process to calibrate and verify the coefficient of new chamber, make sure the focus is correct and working before QC. If the instrument is within QC control, it means the coefficient is correct, if the instrument is out of QC control, it means the coefficient needs to be calibrating on premise of correct and working instrument. 12. Finish QC by using the QC liquid, repeat 10 time each channel, count the item quantity of 100 frames and divide by 100 to get the average quantity per frame. Chamber coefficient=QC Concentration/average quantity per frame. CAUTION: The reference range of chamber coefficient:15.5~19.5, if the coefficient is out of range, please contact AVE service engineer. 112 AVE-764 Service Manual 8.5.3 Replacement of CCD Fig.8-18 CCD Installation Key to figure 8-18 1. CCD 2. CCD cable fixing screw 3. CCD USB interface 4. C flex 5. CCD connector 6. Hand screw CAUTION: Power off the instrument before replacing CCD. CCD replacement operation steps: 1. Use cross screw-driver to unlock the CCD cable fixing screws; 2. Take off the CCD USB cable; 3. Unlock the hand screw(by hand or by tools if needed) until the entire CCD component can be pull out(Fig.8-18 1,4,5); 4. Bring out the new CCD, remove the package and cover; CAUTION: During the installation of CCD, the camera lens must face down, Do not blow to CCD camera lens field in case dirty spots. 5. Install the new CCD in reverse, pay attention to the installation direction(USB interface facing backward), make sure the connection is fixed and tight; 6. Bring back the CCD USB cable and fixing screws; 7. Power on the instrument, check whether the square-mark frame is parallel with the vision field or not, if not, adjust the CCD position and fix the hand screw tightly; 8. After replacement, check the focus and square-mark position under HP of each channel, if the focus is unexpected, please refer to 8.3 Microscope and Image Adjustment to complete the adjustment, make sure focus is in best condition. CAUTION: If dirty spots on CCD is found, please contact AVE service engineer. 113 AVE-764 Service Manual 8.5.4 Replacement of Sensors CAUTION: Power off the instrument before replacing the sensors. All sensors on microscope are correlation type, mainly used for locating the initial position of motors, named after the corresponding position. 1. Replacing objective motor sensor Fig.8-19 Objective motor sensor Key to figure 8-19 1.I type nut M2.5 2.φ2.5 spring washer 3.φ2.5flat washer 4. Objective motor sensor 5. M2.5X6cross pan-head screw As shown in Fig.8-19, tearing-down starts with the fixing screws, unlock and take off the sensor, unplug the cables, replace a new sensor, put it back and fix. 2. Replacing Left/right motor sensor 114 AVE-764 Service Manual Fig.8-20 Left/right motor sensor Key to figure 8-20 1. Holder; 2. M3 flat-washer; 3. M3 spring-washer; 4. M3*8 cross recess pan-head screw; 5. Left/right motor sensor ; 6. M2.5*6 cross pan-head screw; As shown in Fig.8-20, tearing-down starts with the fixing screws, unlock and take off the sensor, unplug the cables, replace a new sensor, put it back and fix. 3. Replacing Focus lamp motor sensor Fig.8-21 Focus lamp motor sensor Key to figure 8-21 1. M2.5*6 cross pan-head screw 2. Focus lamp motor sensor As shown in Fig.8-21, tearing-down starts with the fixing screws, unlock and take off the sensor, unplug the cables, replace a new sensor, put it back and fix. 4. Replacing forth/back motor sensor 115 AVE-764 Service Manual Fig.8-22 Forth/back motor sensor Key to figure 8-22 1. Forth/back motor sensor 2. M2.5*6 cross pan-head screw As shown in Fig.8-22, tearing-down starts with the fixing screws, unlock and take off the sensor, unplug the cables, replace a new sensor, put it back and fix. 5. Replacing up/down motor sensor Fig.8-23 Up/down motor sensor Key to figure 8-23 1. M2.5*8 cross pan-head screw 2. M2.5 spring washer 3. M2.5 flat washer 4. Shelter 116 AVE-764 Service Manual 5. Narrow-groove sensor 6. M2.5*6 cross pan-head screw As shown in Fig.8-23, tearing-down starts with the fixing screws, unlock and take off the sensor, unplug the cables, replace a new sensor, put it back and fix. 8.5.5 Replacement of microscope main control board Fig. 8-24 Microscope main control board Key to figure 8-24 1. Main control board If it’s needed to be replaced, open the rear panel, unplug all the connecting cables, remove all the fixing screws, replace a new one, put back all the cables and plugs correspondingly in right direction. CAUTION: After replacement, it’s necessary to confirm the Lower-computer program version, the version must be as same as former version. If not, see 10.3 Upgrading the module firmware for details. 117 AVE-764 Service Manual 9 TROUBLESHOOTING 9.1 Sampler error message No. 1 2 Symptom After starting the examination, the system notice as ‘Sampler communication timeout’. There is no rack loaded at ready area but the Pawls keep pushing forward and back, meanwhile the system notices as ‘Push-in motor at ready area reaching position error.’ Probable cause Recommended action The communication cable connected with sampler was loosen or not well connected. Re-connect the cable. Check whether the switch located at left side of sampler is turn on. If not, turn on the switch. The COM port number was not well defined or was changed. The default COM port number of sampler is COM4. Open a COM tools (sscom32E.exe) to find current port communicated with sampler and if necessary, change it to COM4. Launch the system and go to Management-System Settings-COM setting, check whether the COM port number of sampler is COM4. The control module of sampler was damaged. See 5.5.4 Main control board replacement to replace the control module. Check whether the sampler is placed at a direct strong light area. If so, prevent the sampler from strong light. There is dirt stick on the surface of photoelectric sensor (JR1, JR2). The sensitivity of JR2 is too low or was invalid. The control module of sampler was damaged. 118 Power off the sampler and Use a cotton swab moistened with absolute alcohol to swab the interface to cleanup. See 5.3.2 Reflective photoelectric sensor adjustment to increase the sensitivity of JR2. If the issue persists, see 5.5.1 Reflective photoelectric sensor replacement to replace JR2. See 5.5.4 Main control board replacement to replace the control module. AVE-764 No. 3 4 5 6 Service Manual Symptom The system notices as ‘Sensor to detect racks loaded at bridge area error.’ The rack at testing area can not be shifted by two hooks. Sample was missed examination by instrument. The empty position on rack was mistakenly identified. Probable cause Recommended action JF1 error. See 5.3.4 Correlation photoelectric sensor testing to test JF1, if JF1 do have problem, see 5.5.2 Correlation photoelectric sensor replacement to replace it. Motor MJ4 error. See 5.3.3 Motor testing to test MJ4, if MJ4 do have problem, see 5.5.5 Motor replacement to replace it. The control module of sampler was damaged. See 5.5.4 Main control board replacement to replace the control module. Check whether the tube rack is for specialized use. Change the tube rack if it is not. The hooks may be obstructed by dirt or deposits. Check the flexibility of hooks. Moisten cotton swabs with disinfectant/cleanser to cleanup, remove any deposits. The control module of sampler was damaged. See 5.5.4 Main control board replacement to replace the control module. The sensitivity of JR8 is too low or was invalid. See 5.3.2 Reflective photoelectric sensor adjustment to increase the sensitivity of JR8. If the issue persists, see 5.5.1 Reflective photoelectric sensor replacement to replace JR8. The control module of sampler was damaged. See 5.5.4 Main control board replacement to replace the control module. There is dirt stick on the surface of JR8. Power off the sampler and Use a cotton swab moistened with absolute alcohol to swab the interface to cleanup. JR8 was not located right opposite the middle of test tube surface. Remove the cover of sampler to adjust JR8 to the standard position. Check whether the sampler is placed at a direct strong light area. If so, prevent the sampler from strong light. The sensitivity of JR8 is too See 5.3.2 Reflective photoelectric 119 AVE-764 No. Service Manual Symptom Probable cause high. 7 8 9 There is no rack loaded at tested area but the pawls keep pushing. Meanwhile the system notice as ‘Push-out motor at tested area reaching position error.’ There is no rack loaded at tested area or the tested area is not full, but the system notices as ‘Tested area is full.’ The system notices as ‘Loading motor at bridge area home position error.’ Recommended action sensor adjustment to decrease the sensitivity of JR8. The control module of sampler was damaged. See 5.5.4 Main control board replacement to replace the control module. Check whether the sampler is placed at a direct strong light area. If so, prevent the sampler from strong light. There is dirt stick on the surface of JR6. The sensitivity of JR6 is too high. Power off the sampler and Use a cotton swab moistened with absolute alcohol to swab the interface to cleanup. See 5.3.2 Reflective photoelectric sensor adjustment to decrease the sensitivity of JR6. The control module of sampler was damaged. See 5.5.4 Main control board replacement to replace the control module. Check whether the sampler is placed at a direct strong light area. If so, prevent the sampler from strong light. There is dirt stick on the surface of JR4. Power off the sampler and Use a cotton swab moistened with absolute alcohol to swab the interface to cleanup. The sensitivity of JR4 is too high. See 5.3.2 Reflective photoelectric sensor adjustment to decrease the sensitivity of JR4. The control module of sampler was damaged. See 5.5.4 Main control board replacement to replace the control module. Check whether the wire of JS4 connected with control module was loosening. Re-connect it if necessary. JS4 error or the control module was damaged. See 5.3.5 Inching photoelectric sensor testing to test JS4. Replace it if JS4 did have problem. See 5.5.4 Main control board replacement to replace the 120 AVE-764 No. Service Manual Symptom Probable cause Recommended action control module. 10 11 12 13 The system notice as ‘Push-in motor at ready area home position error.’ The system notice as ‘Push-in motor at ready area home position error.’ The system notice as ‘Push-out motor at tested area home position error.’ The system notice as ‘Loading motor at bridge area mechanical error.’ Check whether the wire of JS1 connected with control module was loosening. Re-connect it if necessary. JS1 error or the control module was damaged. See 5.3.5 Inching photoelectric sensor testing to test JS1. Replace it if JS4 did have problem. See 5.5.4 Main control board replacement to replace the control module. Check whether the wire of JS2 connected with control module was loosening. Re-connect it if necessary. JS2 error or the control module was damaged. See 5.3.5 Inching photoelectric sensor testing to test JS2. Replace it if JS4 did have problem. See 5.5.4 Main control board replacement to replace the control module. Check whether the wire of JS3 connected with control module was loosening. Re-connect it if necessary. JS3 error or the control module was damaged. See 5.3.5 Inching photoelectric sensor testing to test JS3. Replace it if JS4 did have problem. See 5.5.4 Main control board replacement to replace the control module. The belt of motor (MJ4) is too tight. Adjust the belt adhesion to be within operational limits. The guide of motor was damaged. Replace the guide. See 5.3.3 Motor testing to check whether MJ4 was damaged. See 5.5.5 Motor replacement to replace MJ4. The control module of sampler See 5.5.4 Main control board 121 AVE-764 No. 14 15 16 Service Manual Symptom The system notice as ‘Push-in motor at ready area mechanical error.’ The system notice as ‘Sample-switchi ng motor at testing area mechanical error.’ The system notice as ‘Push-out motor at tested area mechanical error.’ Probable cause Recommended action was damaged. replacement to control module. replace the The belt of motor (MJ1) is too tight. Adjust the belt adhesion to be within operational limits. The guide of motor was damaged. Replace the guide. See 5.3.3 Motor testing to check whether MJ1 was damaged. See 5.5.5 Motor replacement to replace MJ4. The control module of sampler was damaged. See 5.5.4 Main control board replacement to replace the control module. The belt of motor (MJ2) is too tight. Adjust the belt adhesion to be within operational limits. The guide of motor was damaged. Replace the guide. See 5.3.3 Motor testing to check whether MJ2 was damaged. See 5.5.5 Motor replacement to replace MJ2. The control module of sampler was damaged. See 5.5.4 Main control board replacement to replace the control module. The belt of motor (MJ3) is too tight. Adjust the belt adhesion to be within operational limits. The guide of motor was damaged. Replace the guide. See 5.3.3 Motor testing to check whether MJ3 was damaged. See 5.5.5 Motor replacement to replace MJ3. The control module of sampler was damaged. See 5.5.4 Main control board replacement to replace the control module. 9.2 Pump-valve module error message No. 1 Symptom There is bubble existing in the channel after inhaling sample or the sample can not be Probable cause Recommended action Check whether the sampling needle was blocked. See 7.4 Maintenance to dredge Sampling needle. Check whether the counting chamber was blocked. See 6.4.4 Counting Chamber Maintenance for further process. Check whether there is See 6.1 Summary to check the 122 AVE-764 No. 2 3 Service Manual Symptom Probable cause inhaled into the channel. The system notice as ‘Sampling channel leakage or blockage.’ broken or leakage pipelines. Check all the connectors between two pipelines. pipelines according the piping diagram. If any problems are found, see 6.4.3 Other pipelines Maintenance for further process. Check all pinch valve pipelines to see whether anyone was broken. See 6.4.2 Valve-tube Maintenance to replace the broken pinch valve pipelines. Pinch valves error or waste pump (MJ2) error. See 6.3.1 Pump & valve testing to test whether pinch valves or waste pump (MJ2) have problem. Replace the error components if necessary. Pump tube of MJ2 was broken or aging. See 6.5.1 Peristaltic-pump tube replacement to replace the pump tube. The parameters related to sampling are not correct or changed. See 6.3.2 Pump-Valve Calibration to perform sampling pump calibration. Check whether the sampling needle was blocked. See 7.4 Maintenance to dredge Sampling needle if necessary. Maybe one of the connector located on the pipeline from sampling needle to the right end of counting chamber is broken or disconnected. See 6.1 Summary to check the pipelines according the piping diagram. If any problems are found, see 6.4.3 Other pipelines Maintenance for further process. Check whether cleanser A was run out. If so, replace a new one. Because the instrument was not use for long time, the pipelines were filled with air. Confirm the error message several times for its own eliminated. Check whether there is broken or leakage pipelines. Check all the connectors between two pipelines. See 6.1 Summary to check the pipelines according the piping diagram. If any problems are found, see 6.4.3 Other pipelines Maintenance for further process. Maybe one of valves was not properly open. See 6.3.1 Pump & valve testing to test whether pinch valves have problem. Replace the error components if necessary. Pump tube of MJ1 was broken or aging. See 6.5.1 Peristaltic-pump tube replacement to replace the pump tube. Though this is a visible positive sample, no cells can be seen on the screen after inhaling into the channel. The system notice as ‘No cleanser A’. 123 Recommended action AVE-764 No. 4 5 6 7 Service Manual Symptom The system notice as ‘No cleanser B’. The system notice as ‘No maintenance cleanser’. The system notice as ‘Cleaning pool drain pipe leakage or blockage’. The system Probable cause Recommended action The washing pump (MJ1) has no action. See 6.3.1 Pump & valve testing to test whether washing pump has problem. Replace the error components if necessary. Check whether cleanser B was run out. If so, replace a new one. The pinch valve pipeline of DZ7 was stuck. See 6.4.2 Valve-tube Maintenance for further process. The valve DZ7 was not properly open. See 6.3.1 Pump & valve testing to test whether DZ7 has problem. Replace it if necessary. Check whether maintenance cleanser was run out. If so, replace a new one. The pinch valve pipeline of DZ8 was stuck. See 6.4.2 Valve-tube Maintenance for further process. The valve DZ8 was not properly open. See 6.3.1 Pump & valve testing to test whether DZ8 has problem. Replace it if necessary. Check whether the waste pipeline was block or pressed. See 6.1 Summary to check the pipelines according the piping diagram. If any problems are found, see 6.4.3 Other pipelines Maintenance for further process. Outlet at the bottom of wash pool was block. See 6.4.5 Wash pool Maintenance for further process. Pump tube of MJ2 was broken or aging. See 6.5.1 Peristaltic-pump tube replacement to replace the pump tube. The wire connected between wash pool with main control module was loosen or disconnected. See 10.1.3 Sampling Module Diagram to check whether the wire was loosen. Re-connect it if necessary. Sensor detecting upper level of wash pool was invalid. See 6.4.5 Wash pool Maintenance for further process. Waste pump (MJ2) has no action. See 6.3.1 Pump & valve testing to test whether MJ2 has problem. Replace it if necessary. Main control module was damaged. See 6.2.1 Main control board (02A) to replace it. Check whether the waste Discard waste according to 124 AVE-764 No. Service Manual Symptom notice as ‘Waste container is full’. 8 9 10 11 After inhaling sample into the channel, the sample appears not be still but flowing. Cross-contamina tion The system notice as ‘Reagent communication timeout!’ The system notice as ‘Cleaning pool overflow’. Probable cause Recommended action container is full. applicable regulations. The waste sensor gave a false alert. Clean the waste sensor. The pinch valve pipelines are aging thus can not be completely clamped. See 6.4.2 Valve-tube Maintenance for further process, replace it if necessary. Check whether the pinch valves on both sides of counting chamber can not be properly close. See 6.3.1 Pump & valve testing to test whether any valve has problem. Replace the error components if necessary. Check whether the sampling needle was blocked. See 7.4 Maintenance to dredge Sampling needle. Check whether the counting chamber was blocked. See 6.4.4 Counting Chamber Maintenance for further process. Inlet on the top of wash pool was blocked. See 6.4.5 Wash pool Maintenance for further process. Other pipelines were blocked. See 6.1 Summary to check the pipelines according the piping diagram. If any problems are found, see 6.4.3 Other pipelines Maintenance for further process. The pump system needs to be calibrated. See 6.3.2 Pump-Valve Calibration to calibrate the pump system. The pump tubes were aging. See 6.3.2 Pump-Valve Calibration to calibrate the pump system. If necessary see 6.5.1 Peristaltic-pump tube replacement to replace the pump tubes. The reagent cable was not well connected or damaged. Re-connect the cable or replace it. The reagent sensor was damaged. Replace a new sensor. The extend control module (02B) was damaged. Replace the extend control module. Check whether the waste pipeline was block or pressed. See 6.1 Summary to check the pipelines according the piping diagram. If any problems are found, see 6.4.3 Other pipelines Maintenance for further process. Outlet at the bottom of wash See 6.4.5 Wash pool Maintenance 125 AVE-764 No. 12 13 Service Manual Symptom The system notice as ‘Reagent Stop’ while perform pump calibration in Adjustment interface. The system notice as ‘Pump System communication timeout’. Probable cause Recommended action pool was block. for further process. Pump tube of MJ2 was broken or aging. See 6.5.1 Peristaltic-pump tube replacement to replace the pump tube. The wire connected between wash pool with main control module was loosen or disconnected. See 10.1.3 Sampling Module Diagram to check whether the wire was loosen. Re-connect it if necessary. Waste pump (MJ2) has no action. See 6.3.1 Pump & valve testing to test whether MJ2 has problem. Replace it if necessary. Main control module was damaged. See 6.2.1 Main control board (02A) to replace it. Air was inhaled into the pipeline. Re-try several times to eliminate the alert automatically. The detection sensitivity of wash pool was decreased. See 6.4.5 Wash pool Maintenance for further process. Cleanser A is expired or with sediment inside. Replace the expired cleanser A. If sediment is seen please shake well before use. Wire between plug J4 on main control module with main board is not well connected. Re-connect the wire. Main control module is not powered on. Use multimeter to check whether the plug POWER on main control module has +5V and +24v voltage input. If there isn’t, see 4.2.2 Maintenance and Replacement for further process. The COM port number was not well defined or was changed. The default COM port number of pump-valve module is COM3. Open a COM tools (sscom32E.exe) to find current port communicated with pump-valve module and if necessary, change it to COM3. Launch the system and go to Management-System Settings-COM setting, check whether the COM port number of pump-valve module is COM3. Main control module was damaged. See 6.2.1 Main control board (02A) to replace it. 126 AVE-764 No. 14 Service Manual Symptom Probable cause Recommended action The system notice as ‘Valve Test failed!’ while performing valve testing. The software conflicts with the firmware of pump-valve module. Check the firmware version of main control module. If it did conflicts with software please contact the AVE service engineer to ask for the latest version and see 10.3 Upgrading the module firmware to upgrade the firmware. 9.3 Automatic sampler error message No. 1 2 3 Symptom The system notice as ‘Lift motor can't find zero point’ or ‘Level motor can't find zero point’ while launching the system. The system notice as ‘Sampling needle collision’. The system notice as ‘No Probable cause Recommended action The cable connected control module of Automatic sampler with adapter module is loosening. Re-connect the cable. The photoelectric sensor located at vertical zero position error. Clean the sensor. If necessary, see 7.5.4 Replacing up-down photoelectric sensor to replace it. The photoelectric sensor located at horizontal zero position error. Clean the sensor. If necessary, see 7.5.4 Replacing up-down photoelectric sensor to replace it. Control module (02c) of Automatic sampler error. See 7.5.1 Replacing sampling control board (02C) to replace it. Main control module (02A) was damaged. See 6.2.1 Main control board (02A) to replace it. The photoelectric sensor detected collision error. Clean the surface of sensor, if necessary, see 7.5.6 Replacing sampling needle to replace it. Fixed block of sampling needle was loosening cause anti-collision module contact problems. Tighten fixed block. Collision is happened during normal examination. Check whether the test tubes are for specialized use. Change them if they are not. If they are, see 7.3 Adjustment to adjust the sampling needle. The collision was happened at wash pool. See 7.3 Adjustment to adjust the sampling needle. The collision was happened at other area. Remove the obstacles. The sample is not urine. Only urine sample can be detected. There is too little urine in the The minimum volume of sample is 127 AVE-764 No. Service Manual Symptom sample’. Probable cause Recommended action test tube. 2ml. The parameters of sampling needle were not set correctly or were changed. See 7.3 Adjustment to adjust the sampling needle. Level detection error. Re-connect the wire of sampling needle. Under power off status, Using a cotton swab moistened with absolute alcohol to swab the interface of needle. If the issue persists, see 7.5.6 Replacing sampling needle to replace the sampling needle. 9.4 Microscope module error message No. 1 Symptom The focus is unstable. The cells in the images are too dark or too bright, hard to identify. Probable cause Recommended action The LP or HP lens was loosening. Retighten the lens. The installation location is too close to the wall thus heat dissipation of instrument is not well. The mechanism of microscope does be affect by the temperature. Adjust the installation location minimum 30cm away from the wall. The normal examination only can be start after 30 minutes warming-up. The revolving nosepiece is not properly located into the slot. See 8.3.2 Objective Adjustment for adjustment. The sensitivity of the photoelectric sensor was decreased or invalid. Using a cotton swab moistened with absolute alcohol to clean the sensor. The belt of up-down motor was loosening. Adjust the belt adhesion to be within operational limits. The synchronizing wheel of up-down motor was loosening. Tighten the 2 fixed screws. The location of photoelectric sensor or light barrier was moved. Adjust the location of photoelectric sensor and light barrier to make sure the light barrier is right in the middle of groove. After this, see 8.3.5 Focus Adjustment to re-adjust the focus. 128 AVE-764 No. 2 3 4 Service Manual Symptom The background of images became dark or bright. The system notice as ‘Forward-backw ard motor out of range.’ The system notice as ‘Left-right motor out of range;’ Probable cause Recommended action The screw fixed at coarse tuning nut was loosening. Tight the screw to meet the standard: Manually rotate the nut, the focus of cells in the counting chamber is stable. The revolving nosepiece was loosening. Please contact the AVE service engineer. Maybe there is too many dust stick on the surface of lamp, collecting mirror, stage or counting chamber. Cleanup the dust. The parameter of Focus lamp was not correctly set or was changed. The default value of LP is 700 and HP is 200. The parameters of CCD image were NOT correctly set. See 8.3.4 Image Adjustment for adjustment. The gears of collecting mirror motor locked. Lubricate the gears of motor. The lamp was aging. See 8.5.1 Replacement of Micro-lamp to replace the lamp. There is dust stick on the surface of photoelectric sensor, located at the zero position of forward-backward motor. Using a cotton swab moistened with absolute alcohol to clean the sensor. The photoelectric sensor located at the zero position of forward-backward motor error. See 8.5.4 Replacement of Sensors to replace the sensor. The control module of microscope error. See 8.5.5 Replacement of microscope main control board for replacement. There is dust stick on the surface of photoelectric sensor, located at the zero position of left-right motor. Using a cotton swab moistened with absolute alcohol to clean the sensor. The photoelectric sensor located at the zero position of left-right motor error. See 8.5.4 Replacement of Sensors to replace the sensor. The control module of microscope error. See 8.5.5 Replacement of microscope main control board for replacement. 129 AVE-764 No. 5 6 7 8 Service Manual Symptom The system notice as ‘Up-down motor out of range;’ The system notice as ‘Lens motor out of range;’ The system notice as ‘Focus motor out of range.’ LP or HP images captured are the same. Probable cause Recommended action There is dust stick on the surface of photoelectric sensor, located at the zero position of up-down motor. Using a cotton swab moistened with absolute alcohol to clean the sensor. The photoelectric sensor located at the zero position of up-down motor error. See 8.5.4 Replacement of Sensors to replace the sensor. The control module of microscope error. See 8.5.5 Replacement of microscope main control board for replacement. There is dust stick on the surface of photoelectric sensor, located at the zero position of lens motor. Using a cotton swab moistened with absolute alcohol to clean the sensor. The photoelectric sensor located at the zero position of lens motor error. See 8.5.4 Replacement of Sensors to replace the sensor. The control module of microscope error. See 8.5.5 Replacement of microscope main control board for replacement. There is dust stick on the surface of photoelectric sensor, located at the zero position of collecting mirror motor. Using a cotton swab moistened with absolute alcohol to clean the sensor. The photoelectric sensor located at the zero position of collecting mirror motor. See 8.5.4 Replacement of Sensors to replace the sensor. The control module of microscope error. See 8.5.5 Replacement of microscope main control board for replacement. There is dust stick on the drive shaft of left-right motor. Using a cotton swab moistened with absolute alcohol to clean the drive shaft. Lubricate the drive shaft. The tabletting pressed at counting camber bracket were slipped. Replace the tabletting. The control module of microscope error. See 8.5.5 Replacement of microscope main control board for replacement. Left-right motor error. Please contact the AVE service engineer. 130 AVE-764 No. 9 10 11 12 Service Manual Symptom The image area appears blank, blue, black or blurred screen. Or the system notice as ‘Failed to save exposure!’ or ‘Open CCD error!’ Positive or weakly positive samples were examined as negative result. There are spots on the captured images. The system notice as ‘Microscope communication timeout’ Probable cause Recommended action The CCD communication cable was not well connected. Re-connect the cable. The CCD driver was not well installed. Re-install the CCD driver. CCD was damaged. See 8.5.3 Replacement of CCD to replace the CCD and install the driver. The parameter of Dirty Spot was not correctly set. See 6.3.3 Blank Calibration to perform blanking calibration. If the dirty spots are higher than5 and they are impossible to cleanup, see 8.5.2 Replacement of Counting Chamber to replace the counting chamber. The focus was not correctly adjusted. See 8.3 Microscope and Image Adjustment to adjust the focus. The fluid pipeline was blocked. See 8.4 Maintenance to check whether sampling needle, pipelines or counting chamber was blocked, if necessary, perform maintenance on blocked pipeline. The mark of counting chamber was not correctly adjusted. See 8.3.3 Square-Mark Adjustment of Microscope to adjust the mark. The background of image was too dark or too light. See 8.3.4 Image Adjustment to adjust the background. The spots are inside the channels of counting chamber. Inhaling the disinfectant into the channels and soaking for 3 minutes, then perform Wash and Wash+. If the spots are impossible cleanup, see 8.5.2 Replacement of Counting Chamber to replace the counting chamber. The spots are inside the CCD camera. See 8.5.3 Replacement of CCD to replace the CCD camera. The communication cable connected on the control module of microscope was not well connected. See 10.1.4 Microscope Module Diagram to check whether the cable was well connected. Re-connect it if necessary. Power supply of main See 10.1.4 Microscope Module 131 AVE-764 No. Service Manual Symptom Probable cause Recommended action control module is not properly. Diagram to check whether MPW plug was well connected. Use multimeter to check whether the voltage between two pins was normal. If the voltage deviates from standard, see 4.2 Power System to replace the main power module. See 8.5.5 Replacement of microscope main control board for replacement. The control module of microscope error. 9.5 Software error message No. 1 Symptom Probable cause Launch the system and the system notice as ‘Initialization Error!’ An unknown error occurred while communicating with the database. Recommended action 1 2 3 2 3 Login the software, anyone of below messages is noticed. Failed to open LIS port! Failed to open chemistry port! Failed to open microscope port! Failed to open pump port! Failed to open sampler port! Failed to open reagent port! Failed to open barcode port! The COM ports number were not correctly set. The system 661recg.exe is missing. 1 2 3 132 1 Restart the instrument to check whether the problem is eliminated. Make a backup of all parameters and try to uninstall and re-install the database. If the issues persists, contact AVE service engineer. Restart the instrument to check whether the problem is eliminated. Check whether the COM ports are existed. Go to Computer Management-Device Manager-Ports (COM & LPT), and see 3.3.1 COM Setting to check whether the COM ports of every module are existed or changed. Correctly set the COM ports if necessary. If the issues persists, contact AVE service engineer. Check whether 661recg.exe is AVE-764 No. Symptom Service Manual Probable cause notice as ‘No recognition program!’ 4 The system notice as ‘LIS communication failure’ Recommended action 2 The COM port number of LIS is not correctly set or was occupied. 1 2 5 6 7 Login the software, anyone of below messages is noticed. Invalid Serial Number! Invalid Password! Authorization validation failed! An unknown error occurred while communicating with the database. The system notice as ‘Export results to LIS error!’ An unknown error occurred while communicating with the database. The system notice as ‘The instrument is out of control during QC test, adjust the instrument and retry!’ The QC measurement is out of control. The system notice as ‘Abnormal Data:’ An unknown report error occurred installed under D:\AVE. Contact AVE service engineer if the file is not existed. Check whether the COM port number of LIS is correctly set or was occupied. Re-set it if necessary. If the issues persists, contact AVE service engineer. Contact AVE service engineer. 1 2 Make a backup of all parameters and try to uninstall and re-install the database. If the issues persists, contact AVE service engineer. See Chapter 6.2 Remedies on Out-of-control in User’s Manual for further process. 1 2 8 3 133 Copy the folder named Report in update package to replace the old one. If the issues persist, re-install CRRedist2005_x86.msi in the rystalReports folder of software installer. If the issues persist, contact AVE service engineer. AVE-764 No. Service Manual Symptom Probable cause 9 The system notice as ‘Failed to read or write registry!’ An unknown system error occurred 10 Login the system, the system notices as ‘Illegal user’ The watch dog was not installed or damaged. 11 The system notice as ‘Open DB error’. Recommended action 1 2 The database was not properly start. 1 2 Check the system registry file or re-install operation system. If the issues persist, contact AVE service engineer. Reconnect the watch; If the issues persist, contact AVE service engineer. Re-start the database, if the issues persist, re-install the database. 9.6 Other error message No. 1 Symptom After power on the instrument, the power indicator of monitor is flashing but the screen is displaced nothing. Probable cause Recommended action The VGA cable of monitor was not well connected. Re-connect the cable or replace it if necessary. Memory has contact problems or was damaged. Re-connect the Memory or replace it if necessary. The monitor has problems. Replace the monitor. The Main board is damaged. Contact AVE service engineer. The power cable of instrument was not well connected. Re-connect the power cable. The power button is not switch on. Switch on the power button. Re-connect the wire. 2 After pressing the start button, the instrument is not start up. The wire of start button is not well connected. The start button is stuck. The fuse is probably burned out. CAUTION: Beware of electric shock! Plug the power cable before operation! Open the front panel and remove the button-pad, then re-install it back. Replace a new one with the same specifications: T3.15A 250V Φ5×20. CAUTION: Beware of electric shock! Plug the power 134 AVE-764 No. Service Manual Symptom Probable cause Recommended action cable before operation! 3 4 5 Launch the system, the system time is changed to default. The instrument is shut down or restart automatically during examination. The system could not start because the system file is missing or corrupt The PC power module error See 4.2.2 Maintenance and Replacement to replace PC power module. The battery of Main board was dead. Replace the battery. There is too much dust stuck on the CPU fan. Power off the instrument and cleanup the dust. CPU fan error. Replace CUP fan. CPU is overheating. Add thermal grease. The operation system error. Make a backup of database and restore the operation system. The power supply is not stable. UPS device is recommended to use. The system file is missing or corrupt. Restoring the operation system. CAUTION: Contact AVE service engineer if you cannot resolve the issue using this Troubleshooting guide. 135 AVE-764 Service Manual 10 APPENDICES 10.1 Electrical Connection Diagram 10.1.1 Main Diagram 136 AVE-764 Service Manual 10.1.2 Automatic Sampler Diagram 137 AVE-764 Service Manual 10.1.3 Sampling Module Diagram 138 AVE-764 Service Manual 10.1.4 Microscope Module Diagram 10.2 Upgrading the software 10.2.1 Upgrading the software You will need the latest version of the software (a folder named “Update EN [Version]”) provided by AVE. 139 AVE-764 Service Manual To upgrade the software, please follow those steps below. 1. Copy all the files in the Update EN [Version] folder and then paste to the Update files folder which located in AVE SW Update. 2. Run the update program AVE Update.exe, then click Update button to start. 3. After that, the SW will be updated automatically. When the process is finished, click Close button to quit. 4. After you upgrading the SW, it’s necessary to refresh the database in case to see the modified wordings implemented in the SW. Please login the SW as ‘avekf’ user name, with the password ‘ave’. 5. Go to Help-Init Config, and you will need to set up ‘Language’ as ‘0’, then click ‘Save’ button alongside. 6. Quit the SW and re-login the SW. If the User name appeared as Chinese character (as shown below), which means the database was successfully updated. Then please go to next step. Otherwise, please refer to guide for SW reinstallation to refresh the database by re-installing the SW. 7. Re-login the SW as ‘avekf’, with password ‘ave’. 8. Go to Help-Init Config (Chinese version) again, and set back ‘Language’ as ‘1’. Click ‘Save’ button. Quit the SW and re-login it. Then you will finalize refreshing the database and can continue to test. 140 AVE-764 Service Manual 10.2.2 Reinstalling the software There are some software errors that you can only fix by completely removing and reinstalling the software. CAUTION: It’s highly recommended that you make a backup copy of the complete ‘AVE’ folder on a removable drive before completing the following steps. To restall the software, please follow those steps below. 1. You may access AVE-764 installer file named ‘AVE Setup [latest released date]’ from local hard disk at F:\Tools\, or contact AVE service engineer to receive the installer file via e-mail. Save the installer files to the F partition of local hard disk. 2. Login the software, go to Management-Backup and export the Urine Data and Parameters to the directory. 3. Quit the software and stop running Database. Make a copy of the complete folder of software to the directory. 4. Uninstall the software: On the desktop, click Start, click Control Panel, and then double-click Add or Remove program. Find and click AVE v2.0 among the currently installed programs, and then click Remove. If the operating system prompts you to confirm the removal of the program, click Yes. 5. Find and double-click the setup.exe software installer file you saved in Step 1. Follow the instruction that appear on the screen. 6. Login the software and go to Management-Backup, import the Urine Data and Parameters to the directory you export to in Step2. 10.3 Upgrading the module firmware 10.3.1 Module firmware requirements You may need to check if the version of module firmware is the latest one. To check the version, it’s necessary to use a COM tools (sscom32E.exe) by sending the commands below to check. Module COM port Command Pump-valve Module COM3 FC 00 FC Sampler Module COM4 FB 01 FC Module firmware requirements Software version Module firmware version 2.2.8.2015 Microscope Module: XWH763B2.2H.hex 141 AVE-764 Service Manual Pump-valve Module: Stand(2014-10-09)76(B)BF V1.05.hex Sampler Module: 2014-10-30 764SY V3.41 10.3.2 Upgrading the module firmware You will need the latest version of the module firmware provided by AVE service engineer. To upgrade the module firmware, please follow those steps below. 1. Launch the system. 2. Plug the power cable off from the control module which you want to individually upgrade. 3. You may need to use the tool STC_ISP_V486-english.exe which can be found at F:\Tools\SOFTWARE\ STC-ISP-V486-ENG\ to upgrade the firmware. 4. 5. 6. Set the MCU Type and COM ports for the module whose PCB you want to individually upgrade according to the below information. Module COM ports MCU Type Microscope module COM2 STC89C58RD+ Pump-valve module COM3 STC12C5A60S2 Sampler COM4 STC12C5A60S2/ STC12C5A32S2 Click Open-Code-File to browse firmware pre-copied in the device. Click ISP Programming button to start upgrading. Meanwhile, the status area will show the progress. Plug power cable on the control module when the status shows 142 AVE-764 7. Service Manual that powering on. Reboot the device to complete the upgrading. 10.4 Installing or uninstalling the driver of CCD camera You may need to install the driver of CCD camera after replacing a new one. The drive file RZIma.inf can be found in the default installation path F:\Tools\CCD\Driver for 300 20130325\. Just follow the Windows process of driver installation or uninstall to perform your operation. 10.5 Reattaching and reinstalling the Database There are some software errors that you can only fix by reattaching the Database. Complete the steps below to reattach the database. CAUTION: It’s highly recommended that you make a backup copy of the complete ‘AVE’ folder on a removable drive before completing the following steps. 10.5.1 Reattaching the Database Removing the error Database 1. Login the software, go to Management-Backup, and export the Urine Data and Parameters to the directory. 2. Quit the software and go to Start-Microsoft SQL Server-Enterprise Manager. Open SQL Server Group-(Local) (Windows NT)-Databases in turn. 3. Right-click on the error database and click Delete to start removing the database. 143 AVE-764 Service Manual 4. After the removing, click OK and reboot the device. Reattaching the Database 5. Right-click on Databases, in the drop-up menu, click ‘Attach Database…’ option in the sub-menu of All Tasks. 6. In the jump-up interface, click ‘…’ button for attaching database. 144 AVE-764 Service Manual 7. Browse AVE2_Data.MDF which is saved for attaching. click OK button to continue the next step. 8. Click OK button to start attaching the database. 9. If the database attachment is complete successfully, click OK button for confirmation. 145 AVE-764 Service Manual 10. Double-check that the new database named AVE2 already exists under Databases. 11. Login the software again and go to Management-Backup, and import the Urine Data and Parameters from the directory. 146 AVE-764 Service Manual 10.5.2 Reinstalling the Microsoft SQL Server CAUTION: There are some software errors that you can only fix by reinstalling the Microsoft SQL Server. In this case, please contact AVE service engineer for further assistance. Do not uninstall any of system program without prior permission from the AVE service engineer. CAUTION: It’s highly recommended that you make a backup copy of the complete ‘AVE’ folder on a removable drive before your operation. 10.6 Backup and restoring the Operation System It makes sense to make a system backup regularly in case that the operation system is crashed or there are some serious system errors that you can only fix by restoring the operation system or individual partitions of hard disk. 10.6.1 Backup the operation system 1. Reboot the device and select ‘1KEY GHOST v2014.07.18’ in the startup menu, select ‘GHOST, DISKGEN, HDDREG, MHDD, DOS’ in the screen, select ‘GHOST 11.5’ in the next screen, select ‘IDE/SATA’ to switch to the operation interface. 2. To backup the complete individual partition of local hard disk, go to local-Partition-To image, a selection window will pop up. Select the local source drive by clicking on the drive number. Press CLICK to select OK button to continue. 147 AVE-764 Service Manual 3. In the pop-up selection window, select source partition from basic drive, and press CLICK to select OK button to continue. 4. A window for creating the copy name and selecting the partition that copy to will pop up. Press CLICK to select the destination partition and to input the copy name. Press CLICK to select Save button to continue. CAUTION: It’s highly recommended that you copy the image file copy on F partition. Make sure that there is enough capacity remained. 148 AVE-764 Service Manual 5. If the operating system prompts you to confirm the compress image, click ‘Fast’ and then click Yes. 6. If the operating system prompts you that the process is finished successfully, click ‘Reset computer’. 149 AVE-764 Service Manual 10.6.2 Restoring the Operation System CAUTION: It’s highly recommended that you make a backup copy of the files that you want to keep on a removable drive before your operation. Make sure that you have a image file that created before in the local drive or removable drive. 1. Repeat Step 1 in 10.6.1 Backup the operation system. 2. Go to local-Partition-From Image, a selection window will pop up. Select image file that to be restored from source partition. Press CLICK to select Open button to continue. 150 AVE-764 Service Manual 3. In the pop-up selection window, select source partition from image file, and press CLICK to select OK button to continue. 4. In the pop-up selection window, select local destination drive, and press CLICK to select OK button to continue. In the pop-up selection window, select destination partition that you want to overwrite from basic drive, and press CLICK to select OK button to continue. 5. CAUTION: To restore the operation system image file, it’s highly 151 AVE-764 Service Manual recommended that you select C partition as the destination partition. 7. If the operating system prompts you to confirm your operation, after re-check that your operation is right, click ‘Yes’. 8. If the operating system prompts you that the process is finished successfully, click ‘Reset computer’. 152 AVE-764 Service Manual 10.7 Maintenance kit No. Kit Quantity No. Kit Quantity 1 slotted screwdriver 1 13 multimeter 1 2 slotted screwdriver 1 14 portable hard drive 1 3 Phillips screwdriver 1 15 Brush 1 4 Phillips screwdriver 1 16 Cotton swabs 5 Phillips screwdriver 1 17 Nylon ties 6 needle-nose pliers 1 18 Electric iron 1 7 Diagonal cutting plier 1 19 syringe 1 8 tweezers 1 20 rag 9 Chip Clip 1 21 latex gloves 10 Open spanner 1 22 Lubricating oil 11 Hex Wrench 1 23 adhesive 12 test pencil 1 24 Lens wipes 10.8 Recommended spare parts Code Name 101990021 101990018 110012007 205010876 201010809 201010810 202010804 202010805 202010806 LENS 10× LENS 40× BRACKET PLATE CONTROL BOARD (MICROSCOPE) PHOTOELECTRIC SENSOR PHOTOELECTRIC SENSOR 4P COLLECTING MIRROR MOTOR OJ5 LENS MOTOR OJ4 ASCENT & DESCENT MOTOR OJ1 FORWARD & BACKWARD MOTOR OJ2 LEFT & RIGHT MOTOR OJ3 BELT 80MXL BELT 155MXL PULLEY 1A763.4.1-06 PULLEY 2A763.4.1-08 PULLEY 5763.4.1-03 SPRING TABLET LAMP MICROSCOPE CCD CAMERA CONTROL BOARD 02A CONTROL BOARD 02B 203030801 203030802 110084009 110084002 110085008 110085010 110085013 110011019 101110009 105100002 105100005 205040003 205040001 153 Maintenance /Service S S S S M/necessary M/necessary S S S Amount/10 analyzers 1 4 2 S S S S S S S S M/2000hours S S S S (1) 4 (1) 1 1 AVE-764 205040004 205040002 312011002 204020801 204020801 203020802 203020801 204010801 204010802 204010803 204010806 204010805 204010804 204010807 204010808 204010809 204010810 202010802 111000002 111000005 199000040 111000006 111000004 110082001 110855036 110855033 110086002 110082005 110082007 110855037 110855038 110855039 110855034 103010005 103010004 201010807 Service Manual CONTROL BOARD 02C ADAPTOR BOARD 02E WASH POOL COMPONENT MAGNETIC VALVE(BIDIRECTIONAL) DZ1 MAGNETIC VALVE(BIDIRECTIONAL) DZ10 CLEANSER PUMP MJ1 WASTE PUMP MJ2 LEFT VALVE (CH1) DZ32 LEFT VALVE (CH2) DZ42 LEFT VALVE (CH3) DZ52 RIGHT VALVE(CH1) DZ31 RIGHT VALVE(CH2) DZ41 RIGHT VALVE(CH3) DZ51 CLEANSER A VALVE DZ9 CLEANSER B VALVE DZ7 MAINTENANCE CLEANSER VALVE DZ8 AIR VALVE DZ2 CLEANSER DETECTIVE COMPONENT PUMP TUBE CHAMBER PIPELINE WASTE PIPELINE PINCH VALVE PIPELINE Φ1.6*3.2 PINCH VALVE PIPELINE Φ2*4 REDUCING CONNECTOR BULKHEAD CONNECTOR NUT RUHR CONNECTOR PMLF31 RUHR CONNECTOR LM41 T-CONNECTOR Y-CONNECTOR GREEN NYLON COLOR BANDS BLUE NYLON COLOR BANDS BLACE NYLON COLOR BANDS POLYPROPYLENE COLOR BANDS FORWARD&BACKWARD MOTOR ASCENT&DESCENT MOTOR ASCENT&DESCENT ZERO POSITION PHOTOELECTRIC SENSOR 154 S S S 1 (1) S S S S S S S S S S S S S S S M/6 months M/necessary M/necessary M/necessary M/necessary M/necessary M/necessary M/necessary M/necessary M/necessary M/necessary M/necessary M/necessary M/necessary M/necessary S S 4 4 4 4 4 10 10 10 10 10 10 10 10 10 10 M/necessary 4 AVE-764 201010808 205040005 102100054 201020821 110084033 110084032 205030001 205030002 205030003 205030004 114990017 110081015 110081012 110084031 110084028 203010714 203010715 101090025 101090011 205030005 105010002 105060002 105020002 105030005 105041003 105081005 105110003 105110004 105050004 110063001 110058009/ 10/11 Service Manual FORWARD&BACKWARD ZERO POSITION PHOTOELECTRIC SENSOR ADAPTOR BOARD 02D SAMPLING ARM CABLE SAMPLING NEEDLE BELT 189MXL025-236Z189MXL025-236Z BELT 165MXL025-206Z165MXL025-206Z CONTROL BOARD 03A (SAMPLER) PHOTOELECTRIC SENSOR CA (JR2,JR3,JR5,JR6) PHOTOELECTRIC SENSOR CB (JR1,JR4) PHOTOELECTRIC SENSOR CC (JR8) COLLISION BEAD LINEAR SLIDES COMPONENT (MR9MNSS1V0N-190L-5-5-1) LINEAR SLIDES COMPONENT (MR9MNSS1V0N-80L-10-10-1) BELT 146MXL025146MXL025 BELT 64MXL02564MXL025 MOTOR 35 MOTOR 42 POWER SWITCH PHOTOELECTRIC SENSOR (JS1,JS2,JS3,JS4) PHOTOELECTRIC SENSORI (JF1, JF2) MAIN BOARD M/necessary 4 S S M/necessary 1 2 2 S S S 1 M/necessary 4 M/necessary 4 M/necessary 4 S S S S S S S S M/necessary 4 M/necessary 4 S 1* PC POWER MODULE CPU INTEL G2020 MEMORY 2GB HARD DISK CPU FAN KEYBOARD MOUSE MONITOR CHASSIS S S S S S S S S S PANEL S 155 AVE-764 102061032 110011029 113080009 101100003 110063012 205010846 109060004 205010802 101990041 105081010 101090005 205010803 106010032 Service Manual WASTE DETECTION CABLE COUNTING CHAMBER (3 CHANNELS) TEST TUBE 16MM*10CM FUSE T3.15A Φ5×20 TEST TUBE RACK WATCH DOG BICONVEX MIRROR LEVEL DETECTION MODULE BARCODE READER FAN 120*120 MAIN POWER SWITCH PC POWER PINBOARD POWER MODULE x=Important on stock (x)=recommended on stock x*=if option installed S M/necessary Consumable S S S S S S S S S S (2) 2 (1) 10.9 Operating AVE-764 and urine analyzer together Before start operating the two analyzers as a unit, their data cables need to be linked and/or their rack movers need to be connected. Consult AVE service engineer for further assistance. Make sure that the system time of urine analyzer is same with that of AVE-764. To operate AVE-764 and urine analyzer together(Urilyzer Auto for example) 1. 2. Install the Uritrail. Connect Urilyzer Auto to AVE-764 with a serial COM cable as shown in below figure. 156 AVE-764 Service Manual 3. Login the software and go to Management-System Settings-COM Setting, click Urine Analyzer and at the right area configure Bit rate, Data, Parity and Stop that the same with Urilyzer Auto. Click Save button then quit. 4. Go to Management-Chemistry Setting-Chemistry Item, configure chemistry items in Parameter and Abbreviation columns that same with Urilyzer Auto. 5. Go to Management-Chemistry Setting-Chemistry Protocol, choose Urilyzer Auto at Apply area, then click Save button at Apply area. Meanwhile, keep the four options (Start Symbol, Transfer ID, End Symbol, Transfer Date) unchosen. 157 AVE-764 6. 7. Service Manual Click Test button, then transfer a result from Urilyzer Auto to verify if the connection is proper. If the data can display at the testing area, go to the next step. If there is no data displayed, please check the COM cable connection and the communication settings. Login microscopy interface and start examination, transfer a result from Urilyzer Auto. Check whether the result can be displayed. Contact AVE service engineer if the communication is not proper. 158