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AVE-764
Service Manual
AVE-764 Series Urine Formed Elements Analyzer
Service Manual
AVE SCIENCE & TECHNOLOGY CO., LTD.
Building B6, Luguyuyuan, No.27 Wenxuan Road, Changsha Hi-tech Industrial Development Zone, Changsha,
Hunan 410205, P.R.China
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The information in this manual was correct at the time of printing.
However, AVE SCIENCE & TECHNOLOGY continues to improve its products and reserves
the right to change specifications, equipment, and maintenance procedures at any time
without notice.
Companies, names and data used in examples are fictitious unless otherwise noted. No
part of this document may be reproduced or transmitted in any form or by any means,
electronic, mechanical or otherwise, for any purpose, without the express written permission
of AVE. AVE may have patents or pending patent applications, trademarks, copyrights or
other intellectual or industrial property rights covering this document or subject matter in this
document. The furnishing of this document does not give a license to these property rights
except as expressly provided in any written license agreement from AVE.
If this instrument is used in a manner differently than specified in this manual, the protection
provided by the equipment may be impaired.
Service Manual for software version 2.2.8.2015
Modification history
Version
Date
Modification
1.1
31.12.2013
First edition
1.2
31.10.2014
Updated for software version V2.1.12.2014
1.3
20.05.2016
Updated for software version V2.2.8 2015
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Index
1 INTRODUCTION ........................................................................................................................... 7
1.1 Intended Use ....................................................................................................................... 7
1.2 How to use the Service Manual .......................................................................................... 7
1.3 Precautions and Warnings Symbols – Definitions .............................................................. 7
1.3.1 Cautions .................................................................................................................... 7
1.3.2 Warnings ................................................................................................................... 7
1.3.3 Biological Warnings................................................................................................... 7
1.4 Symbols ............................................................................................................................... 8
1.5 Contact Information ............................................................................................................. 9
1.6 Limitation of Liability ............................................................................................................ 9
1.7 Use of Third Party Computer Products ............................................................................... 9
2 INSTALLATION ........................................................................................................................... 10
2.1 Summary ........................................................................................................................... 10
2.2 Installation Requirements .................................................................................................. 10
2.2.1 Space ...................................................................................................................... 10
2.2.2 Power ...................................................................................................................... 10
2.2.3 Environmental requirements ................................................................................... 10
2.2.4 Installation ............................................................................................................... 11
2.3 Shipping Cartons ............................................................................................................... 11
2.3.1 Packing List ............................................................................................................. 11
2.3.2 Unpacking the device .............................................................................................. 14
2.4 Installation the device ........................................................................................................ 21
2.4.1 Unscrew microscope fixing plate ............................................................................ 21
2.4.2 Adjust the level of the instrument ............................................................................ 22
2.4.3 Installing the automatic sampler ............................................................................. 23
2.4.4 Installing the Counting Chamber ............................................................................ 25
2.4.5 Installing the watch-dog .......................................................................................... 26
2.4.6 AVE-764 System Connection ................................................................................. 27
2.4.7 AVE-764 reagent connection .................................................................................. 27
2.4.8 Connecting the power cable ................................................................................... 28
2.4.9 Connecting Urine Analyzer ..................................................................................... 28
2.4.10 Power-on ............................................................................................................... 28
2.4.11 Adjustment ............................................................................................................ 28
2.4.12 Installation Checklist ............................................................................................. 32
3 SETUP ......................................................................................................................................... 33
3.1 LIS Setting ......................................................................................................................... 33
3.1.1 Data In Setting ........................................................................................................ 33
3.1.2 Data Out Setting...................................................................................................... 34
3.2 Barcode Setting ................................................................................................................. 35
3.3 System Setting .................................................................................................................. 36
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3.3.1 COM Setting ............................................................................................................ 37
3.3.2 Microscopy Setting .................................................................................................. 37
3.3.3 Channel Setting....................................................................................................... 38
3.3.4 Image Setting .......................................................................................................... 40
3.3.5 Microscopy Setting .................................................................................................. 40
3.3.6 Morphology Setting ................................................................................................. 41
3.4 Operator Management ...................................................................................................... 41
4 AVE-764 ANALYZER .................................................................................................................. 43
4.1 Components ...................................................................................................................... 43
4.2 Power System ................................................................................................................... 48
4.2.1 Components ............................................................................................................ 48
4.2.2 Maintenance and Replacement .............................................................................. 48
4.3 Barcode Reader ................................................................................................................ 50
4.4 Cooling Fans & Dust Cover ............................................................................................... 52
4.5 COM Board........................................................................................................................ 54
5 AUTOMATIC SAMPLER ............................................................................................................. 55
5.1 Summary ........................................................................................................................... 55
5.2 Component ........................................................................................................................ 55
5.2.1 Function areas ........................................................................................................ 55
5.2.2 Electrical control system ......................................................................................... 56
5.2.3 Electric layout .......................................................................................................... 56
5.3 Adjustment ......................................................................................................................... 60
5.3.1 Mechanical testing .................................................................................................. 60
5.3.2 Reflective photoelectric sensor adjustment ............................................................ 60
5.3.3 Motor testing ........................................................................................................... 63
5.3.4 Correlation photoelectric sensor testing ................................................................. 64
5.3.5 Inching photoelectric sensor testing ....................................................................... 64
5.4 Maintenance ...................................................................................................................... 65
5.4.1 Daily maintenance of automatic sampler ................................................................ 65
5.4.2 Reflective photoelectric sensor maintenance ......................................................... 65
5.5 Replacement ..................................................................................................................... 66
5.5.1 Reflective photoelectric sensor replacement .......................................................... 66
5.5.2 Correlation photoelectric sensor replacement ........................................................ 66
5.5.3 Inching photoelectric sensor replacement .............................................................. 67
5.5.4 Main control board replacement ............................................................................. 68
5.5.5 Motor replacement .................................................................................................. 69
6 PUMP & VALVE SYSTEM MODULE .......................................................................................... 70
6.1 Summary ........................................................................................................................... 70
6.2 Components ...................................................................................................................... 71
6.2.1 Main control board (02A) ........................................................................................ 71
6.2.2 Extend Controller Module Components .................................................................. 72
6.2.3 Triple Valve Components ........................................................................................ 73
6.2.4 Wash pool ............................................................................................................... 73
6.3 Adjustment ......................................................................................................................... 74
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6.3.1 Pump & valve testing .............................................................................................. 75
6.3.2 Pump-Valve Calibration .......................................................................................... 76
6.3.3 Blank Calibration ..................................................................................................... 78
6.4 Maintenance and servicing................................................................................................ 78
6.4.1 Quit and Maintain .................................................................................................... 78
6.4.2 Valve-tube Maintenance ......................................................................................... 78
6.4.3 Other pipelines Maintenance .................................................................................. 79
6.4.4 Counting Chamber Maintenance ............................................................................ 79
6.4.5 Wash pool Maintenance ......................................................................................... 79
6.5 Replacement ..................................................................................................................... 80
6.5.1 Peristaltic-pump tube replacement ......................................................................... 80
6.5.2 Disassembly diagram of the pumps and valves ..................................................... 84
6.5.3 Triple valve replacement ......................................................................................... 89
7 SAMPLING MODULE .................................................................................................................. 91
7.1 Summary ........................................................................................................................... 91
7.2 Components ...................................................................................................................... 91
7.3 Adjustment ......................................................................................................................... 92
7.4 Maintenance ...................................................................................................................... 94
7.5 Replacement ..................................................................................................................... 94
7.5.1 Replacing sampling control board (02C) ................................................................ 94
7.5.2 Replacing sampling pinboard (02D) ....................................................................... 95
7.5.3 Replacing back-forth photoelectric sensor.............................................................. 95
7.5.4 Replacing up-down photoelectric sensor ................................................................ 96
7.5.5 Replacing motors .................................................................................................... 97
7.5.6 Replacing sampling needle..................................................................................... 97
8 MICROSCOPE MODULE .......................................................................................................... 100
8.1 Summary ......................................................................................................................... 100
8.2 Components .................................................................................................................... 100
8.3 Microscope and Image Adjustment ................................................................................. 102
8.3.1 Preparation for microscope focus adjustment ...................................................... 102
8.3.2 Objective Adjustment ............................................................................................ 102
8.3.3 Square-Mark Adjustment of Microscope............................................................... 103
8.3.4 Focus Adjustment ................................................................................................. 104
8.3.5 Image Adjustment ................................................................................................. 105
8.4 Maintenance .................................................................................................................... 106
8.4.1 Cleaning of Counting-chamber and stage ............................................................ 106
8.4.2 Cleaning of Sensors .............................................................................................. 106
8.4.3 Cleaning of Micro-lamp and Focus lamp .............................................................. 106
8.4.4 Maintenance of rotation axis ................................................................................. 106
8.4.5 Maintenance of counting chamber........................................................................ 107
8.5 Replacement ................................................................................................................... 107
8.5.1 Replacement of Micro-lamp .................................................................................. 107
8.5.2 Replacement of Counting Chamber ..................................................................... 109
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8.5.3 Replacement of CCD ............................................................................................ 113
8.5.4 Replacement of Sensors....................................................................................... 114
8.5.5 Replacement of microscope main control board .................................................. 117
9 TROUBLESHOOTING............................................................................................................... 118
9.1 Sampler error message ................................................................................................... 118
9.2 Pump-valve module error message ................................................................................ 122
9.3 Automatic sampler error message .................................................................................. 127
9.4 Microscope module error message ................................................................................. 128
9.5 Software error message .................................................................................................. 132
9.6 Other error message ....................................................................................................... 134
10 APPENDICES .......................................................................................................................... 136
10.1 Electrical Connection Diagram ...................................................................................... 136
10.1.1 Main Diagram ...................................................................................................... 136
10.1.2 Automatic Sampler Diagram ............................................................................... 137
10.1.3 Sampling Module Diagram.................................................................................. 138
10.1.4 Microscope Module Diagram .............................................................................. 139
10.2 Upgrading the software ................................................................................................. 139
10.2.1 Upgrading the software ....................................................................................... 139
10.2.2 Reinstalling the software ..................................................................................... 141
10.3 Upgrading the module firmware .................................................................................... 141
10.3.1 Module firmware requirements ........................................................................... 141
10.3.2 Upgrading the module firmware.......................................................................... 142
10.4 Installing or uninstalling the driver of CCD camera ....................................................... 143
10.5 Reattaching and reinstalling the Database ................................................................... 143
10.5.1 Reattaching the Database .................................................................................. 143
10.5.2 Reinstalling the Microsoft SQL Server ................................................................ 147
10.6 Backup and restoring the Operation System ................................................................ 147
10.6.1 Backup the operation system.............................................................................. 147
10.6.2 Restoring the Operation System ......................................................................... 150
10.7 Maintenance kit ............................................................................................................. 153
10.8 Recommended spare parts ........................................................................................... 153
10.9 Operating AVE-764 and urine analyzer together .......................................................... 156
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1 INTRODUCTION
1.1 Intended Use
The AVE-764 is a fully automated analyzer for measuring formed elements in
urine. The analyzer is designed specifically for professional use, as an In Vitro
Diagnostic (IVD) device used by qualified physicians and laboratory staff. The
analyzer is intended for use in screening to assist diagnosis for urinary system
diseases, kidney diseases, circulatory system diseases or infectious diseases.
The AVE-764 can be used as a stand-alone device for microscopic analysis, and
the results from the AVE-764 can also be combined with urine chemistry results in an
integrated report.
1.2 How to use the Service Manual
The Service Manual is intended to explain the instrument in detail, to be a training
guide for authorized technician and to be a troubleshooting reference manual.
This manual is designed for authorized technician who will maintain and repair
the analyzer and must have a thorough knowledge of it. It also specifically references
information concerning analyzer components, operation, theory, utility and
performance.
1.3 Precautions and Warnings Symbols – Definitions
The Service Manual includes information and warnings that must be observed by
the user to ensure a safe operation of the system.
1.3.1 Cautions
CAUTION: Identifies situations that could lead to an accident or equipment
failure.
CAUTION: Identifies electrical caution! Unplug before handling.
CAUTION: Identifies laser light. A laser is used to read the barcodes only.
Protect eyes from the laser light.
1.3.2 Warnings
WARNING: Identifies potentially hazardous situations that could result in
serious injury to laboratory personnel.
1.3.3 Biological Warnings
Identifies potential biological hazards; please be cautious when using,
cleaning or maintain the equipment and take appropriate protective measures.
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1.4 Symbols
The following is a list of symbols used on the nameplate, product packaging or within
the Service Manual and their corresponding meaning.
NO.
Symbol
Description
Location
Service Manual,
nameplate
Service Manual,
Instrument
Service Manual,
Instrument
1
Cautions
2
Cautions
3
Cautions
4
Warnings
Service Manual
5
Biological hazards
Service Manual,
Instrument
6
Earth terminal, the terminal must be
connected firmly
Instrument
7
Power on
Service Manual
8
Power off
Service Manual
9
Date of manufacture
Nameplate
10
In vitro diagnostic medical device
Nameplate
11
Manufacturer
Nameplate
12
CE Mark
Nameplate
13
Consult instructions for use
Product packaging
14
Fragile
Product packaging
15
Do not use when package damaged
Product packaging
16
Stacking limits
Product packaging
17
Ban rolling
Product packaging
18
Upward
Product packaging
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NO.
Symbol
Service Manual
Description
Location
Keep dry
Product packaging
19
1.5 Contact Information
AVE SCIENCE & TECHNOLOGY CO., LTD.
Building B6, Luguyuyuan, No.27 Wenxuan Road
Changsha Hi-tech Industrial Development Zone,
Hunan 410205, P.R.China
Tel: +86-731-8892 5308
Fax: +86-731-8890 7046
Website: http://www.c-ave.com/en.html
1.6 Limitation of Liability
AVE shall not be liable for any loss of use, revenue or anticipated profits, or for
any consequential or incidental damages resulting from the sale or use of the
products.
1.7 Use of Third Party Computer Products
AVE does not recommend that the computers used as a functional part of the
AVE-764 be employed for performing any software or hardware based applications
other than those specifically furnished to operate and support the AVE-764 system, or
those recommended and offered by AVE specifically as accessories or
enhancements for the AVE-764 system. No other third party application software
should be installed in these computers in addition to those provided or recommended
by AVE, without the expressed approval of AVE Technical Service, in order to avoid
potential performance and reliability problems which can result from incompatibility
factors, errors in use of such software, or software-based “viruses.”
Installation of such third party software, or non-approved electronic cards or other
devices, without advance approval by AVE may affect the terms of or void any
warranty otherwise in effect, covering AVE-764 supplied software and hardware on the
computers and the overall performance and reliability of the entire AVE-764 system.
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2 INSTALLATION
2.1 Summary
Anyone who is not trained by AVE or authorized distributors of AVE may cause
damage to the analyzer when opening the box or installing the instrument. Therefore do
not open the box or install the instrument without an authorized technician being present.
On receiving the analyzer, please check the package carefully to see whether there is
a physical damage. If there is any, please notify your local distributor immediately.
2.2 Installation Requirements
2.2.1 Space
The AVE-764 is a tabletop unit which main unit size is 680(L) x 650(W) x 545(H) mm and
main unit weight is 63kg. Instrument is requiring free space on a countertop of
approximately 150x120 cm, with vertical clearance of at least 30 cm.
2.2.2 Power
Voltage
Frequency
Input power
Fuse
100-240V~
50/60Hz
≤450VA
T3.15A 250V Φ5×20
CAUTION: Insure AVE-764 use dedicated power source, this power source
does not supply other instrument and equipment.
CAUTION: UPS no less than 1500VA or voltage regulator are recommended
for the AVE-764 to maintain system operation if voltage is not stable or power
outage is regular.
CAUTION: The equipment inside fixed covered areas of the instrument are
not user serviceable and may present electrical shock hazards.
WARNING:For continued protection against risk of fire and hazard, replace
only with the same type and rating fuse.
WARNING:Equipment requires connection to protective earth for safety
reasons.
WARNING:The instrument’s main supply inlet is being used as the mains
disconnect device.
2.2.3 Environmental requirements
The instrument is suggested to be installed in the environment as:
•
Normal working temperature: 15°C – 30°C
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•
Relative humidity:20% - 80%;
•
Atmospheric pressure:76 – 106kpa;
•
Do not get close to strong electromagnetic source as not to affect the normal
operation of equipment.
•
Keep the environment as clean as possible. Keep it free from mechanical vibration,
noise and power interruptions.
•
Avoid direct sunlight or exposure to heat or wind.
2.2.4 Installation
CAUTION: The AVE-764 has to be installed by an authorized technician.
2.3 Shipping Cartons
2.3.1 Packing List
AVE-764 package contains 2 boxes: plywood box is main unit package; carton box is
accessories package.
1
Fig.2-1 Main unit Package
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5
2
6
7
3
4
Fig. 2-2 Accessories package
AVE-764 Packing List
Analyzer (including computer, microscope)
1
Automatic sampler
Watch dog
Counting chamber with bracket
Vent cover
Keyboard & Mouse
10X & 40X Lens
LCD monitor
VGA cable
2
Power cable
Monitor base
User guider of monitor
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AVE-764 Packing List
Laser printer
3
USB cable
Power cable
User guide
Driver CDROM
Warranty card
Printing paper
Test tube racks
Test tube
4
Power cable
Waste detector
5
Dustproof sponge
Pump tubes
Waste tube Φ4×Φ6-3000mm
Cleanser tube (Silicone tube -1500mm)
COM cable
PS2 extension cable / VGA extension cable
Reagent COM cable
Reagent container cap (Cleanser A,
Cleanser B & maintenance Cleanser)
Microscope lamp
Fuse(T3.15A Φ5×20)
Label
Sample needle stylet
Counting chamber(3 channels)
Windows7 system software discs
Socket head wrench
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AVE-764 Packing List
Cleanser A Container
6
Waste Container
Maintenance Cleanser Container
7
Cleanser B Container
2.3.2 Unpacking the device
CAUTION: Please check the package carefully to see whether there is
damage or package has been open before. If there is any, please take pictures
as evidence and notify AVE service engineer immediately.
2.3.2.1 Unpacking the main unit package
1.
Loosen all of screws in the cover plate and take them away, then open the cover
plate;
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2.
3.
Service Manual
Fig. 2-3 Take the cover plate away
Take away EPE foam coved on the Automatic sampler, then take out the box packed
counting chamber with bracket and watch-dog, which stick under the EPE form, stay
for installation use.
Fig.2-4 Take out sampling cover plate and counting chamber
Take out Automatic sampler stay for installation use, and take away the two pieces
upper padding foam on the instrument.
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4.
Fig. 2-5 Take out Automatic sampler and upper padding foam
Loosen all of screws in two side plates and take these plates away.
5.
Fig. 2-6 Take away two side plates
Take out instrument and place it on a flat table, then prepare installation.
CAUTION: It takes two adults to lift the AVE-764 main unit onto the table.
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Fig.2-7 Take out instrument
6.
7.
Fig.2-8 Bottom plate
In order to prevent damage to equipment parts due to handling or vibration during
transportation, microscope and CPU fan are all added EPE foam pads, please take
them away before installation.
Open the front cover and left side panel (face to the instrument), take out protection
pads No.2 which installed on CCD upper side.
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8.
Fig.2-9 Front View
Take out the protection pad No.1 which fixed under microscope stage.
9.
Fig.2-10 Under microscope stage
Install the 10X and 40X Lens to the microscope.
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Fig.2-11 10X and 40X Lens
Fig.2-12 Install 10X and 40X Lens
10. Remove the security holder to fix the sample needle.
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Fig.2-13 Security holder for sample needle
11. Open right side panel, take out protection pad No.3 under CPU fan.
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Fig.2-14 Under CPU fan
12. Removed package material such as bottom plates, side plates etc., which stored
together for instrument transfer and repack use;
2.3.2.2 Accessories Package Open Step
Open box and take out all of accessories, check them according to the packing list. If
there has any question please contact local distributor. All accessories should be place
properly for installation and maintenance change use.
2.4 Installation the device
For security, we suggest you read these operating instructions carefully and make
corresponding precautions.
2.4.1 Unscrew microscope fixing plate
Microscope is instrument precision part and in order to prevent damage to microscope
due to handling or vibration during transportation, which fixation with two fixing plates
before leave factory. Please unscrew these two fixing plates before installation.
Operation steps as follows:
Loosen the 4 screws, open left panel.
Face instrument, open the front panel,
can see the two fixing plates under
microscope both ends.
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Loosen the 4 screws on the front-end of
microscope cover plate by long handle
cross screwdriver, but do not remove
them..
Refer to step 3 loosen the 4 screws in
the rear, but do not remove them; install
the left side panel back.
CAUTION: When transporting the instrument, make sure to tighten all screws.
2.4.2 Adjust the level of the instrument
1.
2.
3.
Make certain that the bench top is level
There are four feet at the bottom of the instrument on the four corners. Adjust the
height of the feet to keep the instrument in level. Rotate counterclockwise to descend
(as shown in fig identify direction), and clockwise for ascending.
Make sure all the four feet are in contact the bench top with no suspension.
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Fig. 2-15 Adjust feet
2.4.3 Installing the automatic sampler
1.
After level adjustment, place the automatic sampler in front of the main unit for about
12 cm, and then connect the power supply and communication cable of the sampler
(shown in Fig. 2-16) to the interface on the sampler, as shown in Fig. 2-17.
Fig. 2-16 Power supply and communication cable of sampler
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2.
3.
Service Manual
Fig. 2-17 Connecting the power supply and communication cable
Move the sampler towards the main unit until the tenon balls on each side of it are
in alignment with the mortises on the sampler, and the fixing spindle at the lower
middle of the main unit points the fixing hole of the latter, then slot them together. The
tenon balls and fixing spindle are shown in Fig.2-18.
Fig.2-18 Fixing sampler
Adjust the feet at the bottom of the sampler to make certain the bottoms of the main
unit and the sampler are of the same height. The adjustment is the same as that of
the main unit.
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Fig. 2-19 Instrument
2.4.4 Installing the Counting Chamber
In order to protect the Counting Chamber from damage during transport, it has been taken
apart from the microscope and kept in a dedicated box before transport. Install counting
chamber with bracket on the microscope stage before instrument working.
1. Open instrument front cover, take away 3 white counting chamber connecting plastic
pipes, and take out the counting chamber with bracket from package, then take away
silicone caps which covered on the counting chamber both ends.
2. There are 6 joints on the counting chamber 3 channels both ends, joint them with
counting chamber pipelines according to color-ring separately. Pay attention to that
pipeline should insert to joints 1/2 place, as shown in the figure below.
The counting chamber has one side with two location holes which should be
installed inwards.
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3.
Service Manual
Fig. 2-20 Tubing connection of the Counting Chamber
Clean up the microscope stage, then push the bracket under microscope piece and
allow the two steel balls at both ends of the piece falling into the pits on each side of
the bracket. Make certain the bracket is installed level without any looseness.
Fig.2-21 Fixation counting chamber bracket
2.4.5 Installing the watch-dog
Insert the watch-dog to any USB interface on the back of the instrument. As shown in the
fig.
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Fig.2-22 Installing the watch-dog
2.4.6 AVE-764 System Connection
For stand-alone use, the AVE-764 analyzer connects with other peripherals as shown in
follows Figure 2-23. Ensure connecting in place and firmly.
Fig. 2-23 AVE-764 analyzer and its peripheral connections
2.4.7 AVE-764 reagent connection
WARNING: Operator has the duty to comply with the relevant regulations
set by the region and country about the treatment and discard of expired
reagents and wastes.
WARNING: Reagents may cause some irritant to skin and eyes. Operator
should always comply with the safety operation of laboratory and wear
personal protective equipment (such as protective clothing, gloves, etc.)when
coming contact reagents.
CAUTION: Do not place reagent containers on the Analyzer.
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Service Manual
For stand-alone use, the AVE-764 analyzer is connected with reagent bottles as shown in
Figure 2-24. Ensure connecting in place and firmly.
Fig. 2-24 AVE-764 reagent connection
2.4.8 Connecting the power cable
Plug one end of the provided power cord into the power inlet on the back of the host, and
the other end into the dedicated connection board. To ensure safety, please set the power
switch to “ ”, namely the instrument in a power-off state, and the socket is also in “off”
state before connection.
Connect the power cord of monitor and printer as the same way.
2.4.9 Connecting Urine Analyzer
See 10.9 Operating AVE-764 and urine analyzer together.
2.4.10 Power-on
Power-on operation order of AVE-764:
1. Turn on the UPS (if any).
2. Turn on the power distribution board (if any).
3. Turn on the power switches of printer, monitor, etc.
4. Turn on the main power switch(Green switch on slide, if it’s on, the yellow LED
inside of the Green switch will be lighted).
5. Press the Start button on front-side to enter Windows OS.
2.4.11 Adjustment
Every modules of the instrument will make an initialization after powering on, and any
alarm, abnormal noises, shaking or mechanical failure should not be found during this
processing. The correct processing of initialization should be like below.
1. The original condition of sampling needle is inserting in the wash pool. The
initialization of Automatic sampler should follow this: Once the sampling needle is not
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Service Manual
at original condition, it will move up to its vertical zero position then back to its
horizontal zero position. Finally it will move down to its original position. Once the
sampling needle is at original condition, it will move up to its vertical zero position
then forward and backward to perform horizontal setup. Finally it will move down to its
original position.
2. The initialization of microscope module is intended to setup the five motors (up-down
motor, left-right motor, forward-backward motor, objective motor and Focus lamp
motor) to zero position. If they are already at the original condition, they will not move.
Once any unusual conditions were found, please refer to above processing for trouble
clearance.
CAUTION: The processing of initialization mentioned above only be found
when the instrument is power on, it’s different with the initialization after login
the software.
Accessing the Adjustment screen
After initialization, you need to check and, if necessary, adjust every module following the
sections below. You may need to login the Adjustment screen, which is a screen for
service personnel to test the functionality of the subsystems of the device, and to adjust
part of the preset parameters that control the operation of systems and subsystems.
1. Launch the system;
2. Log-in screen will pop up after launching. The default name ‘Service’ is visible on the
screen, enter ‘password’ (without the inverted commas) as password. Then click
Log in to login.
3. Click Management-Adjustment to enter Adjustment screen.
Fig. 2-25 Adjustment screen
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2.4.11.1 Sampling Module
The Sampling Module consists mainly of sampling arm and pump-valve module.
Adjusting the sampling arm
1. Launch system.
2. Prepare one tube of RBC suspension and place it on the tube rack.
3. Go to Management-Adjustment, click Wash, Wash+, Spe-Wash, Wash All
buttons in turn. Repeat those operations three times to check if all the operations are
correct.
4. Select 1#WPC 1#SPC 2#WPC, 2#SPC, 3#WPC, 3#SPC at pump calibration area
then click Pump button in turn to get the Wash Time and Sample Time of three
channels respectively. Repeat those operations above three times to check if all the
operations are correct. Also check whether the Wash Time can meet its criterion
between 3.5~5s, and Sample Time can meet its criterion between 4~5.5s. If the
results are correct, go to step 5. If the results deviate from criterion. See 6.3
Adjustment for further adjustment.
5. Load the tube rack on ready area of sampler. Click Sample button and wait the rack
be shifted to sampling area. Observe the movement of sampling needle, check
whether the sampling location is right in the middle of tube and pinpoint of
needle is 5mm above the bottom of tube. If the location deviates from the standard,
see 7.3 Adjustment for further adjustment.
2.4.11.2 Automatic sampler
After installing the Automatic sampler, you need to check its performance to make sure it
running properly and prevent undetected situation.
CAUTION: Follow the notes below during installing or testing.
1. Load the rack as close as possible to the right side of ready area.
2. Once the racks were loaded on the Automatic sampler, please do not move or
take away the racks manually in case of unnecessary error.
Adjusting the Automatic sampler
1. Launch system.
2. Prepare three tubes of RBC suspension and place them on the tube rack at regular
intervals.
3. Go to Management-Adjustment and load the rack on the ready area. Click Sample
All button, the rack will be pushed at testing area, and then it will be shifted by two
pawls and will be stopped when the first test tube in the rack reaches at sampling
location. After sampling from the first tube, the rack will be shifted again to finish the
next two tubes sampling. After that the rack will be stopped at the last test tube. Click
Sampler button, the rack will be shifted out from testing area by pawls and be
pushed to tested area. Meanwhile the pawls continue moving to finish this setup.
Repeat above operation five times to check ensure the Automatic sampler running
properly.
4. Quit Adjustment screen and login routine work screen. Click Start button to start
initialization for self-testing the status of every module. The Automatic sampler is also
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6.
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tested by itself. After this, check whether the status of Automatic sampler is Normal in
the Status area.
Under working status, push the empty carrier without tube insert arrived at sampling
location of STAT module. Check whether the Insert STAT window will be jumped out
in the screen. If the window is jumped out, see 5.3.4 Correlation photoelectric sensor
testing to adjust STAT detection photoelectric sensor. On the other hand, push the
carrier with tube insert arrived at sampling location of STAT module and check
whether the window will be jumped out. If the windows is not jumped out, see 5.3.4
Correlation photoelectric sensor testing to adjust STAT detection photoelectric sensor
(J10).
Fig. 2-26 Insert STAT
Under working status, prepare five tubes of RBC suspension and place them on the
tube rack at regular intervals. Load the rack on the Automatic sampler to finish all
examination. After examination, go to Data-Tube Records-Urine Tube Records to
check whether there is any samples were undetected. If there is, see 5.3.2
Reflective photoelectric sensor adjustment to Input end detection photoelectric
sensor (JR8).
2.4.11.3 Microscope Module
After installation, you need to check the performance of microscope to make sure it
running properly and if necessary, to adjust the mark position, focus and quality of image
so that to get clearer images and accurate results.
Adjusting the Microscope Module
1. Launch system.
2. Prepare three tubes of RBC suspension and place them on the tube rack at regular
intervals.
3. Go to Management-Adjustment and load the rack on the ready area. Click Sample
All button to intake sample into three channels. Check whether there is bubble exists
in the channels. See 8.3.3 Square-Mark Adjustment of Microscope to adjust the
square mark to initial position (see below). If the mark can not be adjusted to initial
position in any way, see 8.3 Microscope and Image Adjustment for further
adjustment.
4. Select three channels in turn and click Auto-Focus button to perform focus
auto-adjustment. Check whether the cells are clear. Re-perform auto-adjustment until
the cells are clear and can be identified. Then click save button to save the adjusted
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values. If the focus in any way can not be adjusted well, see 8.3 Microscope and
Image Adjustment.
After adjusting the focus of three channels, Click Wash All button and then quit the
Adjustment screen.
Login routine work screen. Click Start button to start initialization to self-test the
status of every module. The microscope module is also tested by itself. After this,
check whether the status of microscope is Normal in the Status area.
2.4.12 Installation Checklist
Laboratory
Serial number of device
Service engineer
Date
Procedure
Service manual
reference
Unpacking the device
2.3.2 Unpacking the
device
Check Packing List
2.3.1 Packing List
Unscrew microscope fixing plate
2.4.1 Unscrew
microscope fixing
plate
Adjust the level of the instrument
2.4.2 Adjust the
level of the
instrument
Installing the automatic sampler
2.4.3 Installing the
automatic sampler
Installing the Counting Chamber
2.4.4 Installing the
Counting Chamber
Installing the watch-dog
2.4.5 Installing the
watch-dog
AVE-764 System Connection
2.4.6 AVE-764
System Connection
AVE-764 reagent connection
2.4.7 AVE-764
reagent connection
Connecting the power cable
2.4.8 Connecting
the power cable
Connecting Urine Analyzer(as
needed)
2.4.9 Connecting
Urine Analyzer
Power-on
2.4.10 Power-on
Adjustment of Sampling Module
2.4.11.1 Sampling
Module
Adjustment of Automatic sampler
2.4.11.2 Automatic
sampler
Adjustment of Microscope Module
2.4.11.3 Microscope
Module
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3 SETUP
The settings in this manual only can be modified by authorized technicians, the other
settings permitted for the administrator and operator can refer to the user manual.
CAUTION: This chapter is only used for authorized technicians.
CAUTION: Since the setting will directly affect the instrument, once
change, do check out this chapter or contact the service engineer.
3.1 LIS Setting
Select “LIS setting” in the “Management” menu to open the LIS setting screen
CAUTION: Contact AVE service engineer if you cannot realize LIS
communication using the contents descript below.
Fig. 3-1 LIS setting screen
3.1.1 Data In Setting
Clicking on “Data In Setting” to open the setting screen for reading LIS data.
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Fig. 3-2 Data In Setting
Data In Setting screen Instructions:
Database information: Set DB string, type, address and data configuration list.
DB Connection S: Set DB connection string.
DB Type: Select the type of LIS DB to be input. The default DB are SQL Server and
Oracle.
DB Address: Set the address of LIS DB to be input.
DB /Server Name: Set the name or server of LIS DB to be input.
User: Set the name of LIS to be input.
Password: Set the password of LIS to be input.
Save: Save the settings.
Delete: Delete existing connection information.
String Mapping: Set LIS String Mapping. The existing LIS String is default. User can
configure on it.
LIS Table: Set LIS Table.
LIS String: Set LIS String.
Analyzer Table: Set Mapping Analyzer name.
Analyzer String: Set Mapping Analyzer String.
Remark: Set notes.
Set Data in List
1. Set Connection string, complete DB type, address, DB /Server Name, user and
password setting. Click Save to save settings.;
2. Right click to add a new LIS Table in String Mapping, entering String corresponding
to the analyzer to accomplish String Mapping.
3. Click Save to save settings. Please refer to step 2 for operations when need to add or
modify the settings.
3.1.2 Data Out Setting
Click on Data Out setting in Management menu can realize the setting of the data output
to LIS.
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Fig. 3-3 LIS Setting screen
LIS connection setting is mainly for the connection of AVE-764 to LIS in hospitals. It can
be used for data-sending-mode selection and serial port setting. Check connection mode,
LIS COM on the right should be filled out if Export to COM is checked.
Data Out Setting screen instructions:
Mode: Select the data output mode. “Read Xml File”, “Read Database” and “Export to
COM” can be checked.
V Upload: Sample status shows as "V" or verification is saved, the data and results will be
transmitted to LIS system automatically.
All Upload: Any sample status or as soon as the testing results come out, the data and
results will be transmitted to LIS system automatically.
COM: Refer to the serial port setting between AVE-764 and LIS.
Bit rate: Refer to the baud rate used for communication between AVE-764 and LIS. The
default value is 9600 bits.
Data: Refer to the data bits used for communication between AVE-764 and LIS. 8 data is
the default value.
Parity: Refer to the parity bit used for communication between AVE-764 and LIS. The
default parity is “N”.
Stop: Refer to the stop bit used for communication between AVE-764 and LIS. The default
value is “1”.
CAUTION: Click Save after settings have been altered to confirm.
3.2 Barcode Setting
Select Barcode Setting in the Management menu to open the barcode setting screen.
Fig.3-4 Barcode Setting screen
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Barcode Setting screen Instructions:
1. Check the Use Barcode box to use the barcode scanning feature. The system will
take the barcode as the primary matching criterion for chemistry and microscopy
results.
2. The system check internal as default for choosing the integrated barcode reader of
the AVE-764.
3. The system check auto as default for choosing the integrated barcode reader of the
AVE-764.
4. Check Tooltip window box to open it. It will pop up for barcode manual entry when
the instrument fails to scan the barcode automatically. The Tooltip window won’t
open when the instrument fails to scan the barcode without checking the box.
5. The system Check Barcode matching box as default for choosing the integrated
barcode reader of the AVE-764. Barcode matching function will only take effect when
the AVE-764 operating with a urine analyzer together.
6. Click Save to save settings.
7. Click Quit to exit.
3.3 System Setting
Select System Settings in the Management menu to open six secondary submenus:
COM Setting, Microscopy Setting, Channel Setting, Image Setting, Microscope
Setting and Morphology Setting. Click one of them to enter the corresponding setting
screen.
Fig.3-5 System Settings screen
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3.3.1 COM Setting
Select “COM Setting” to open the COM setting screen. On this screen, the communication
serial port settings between the master computer and each internal function module or
external urine chemistry analyzer can be set.
CAUTION: The serial port parameters are set on default, no modification is
recommended.
Fig. 3-6 COM setting screen
Operations:
1. Select the parameter options on the left;
2. Select each parameter in COM, Bit rate, Data, Parity, and Stop box on the right.
3. Click Save after settings done.
4. Click Quit to quit the screen.
3.3.2 Microscopy Setting
Select Microscopy Setting to open the Microscopy Setting screen.
CAUTION: The microscopy parameters are set on default, since the settings
will directly affect the test results, no modification is recommended, or may cause
instrument failure.
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Fig. 3-7 Microscopy Setting screen
Microscopy Setting screen Instructions
Screen time: Set the time between fillings of the cell with the sample to the start of the
primary screen.
Deposit time: Set the time between fillings of the cell with the sample to the start of the
LP scan.
Neg. target: Set the particle number upper limit for negative specimen.
High-pos. target: Set the particle number lower limit for strong positive specimen.
Screen Frames: Set the primary screen frames.
LP Frames: Set the LP screen frames for common specimen.
HP Frames: Set the HP screen frames for common specimen.
LP Frames (HpT): Set the LP screen frames for strong positive specimen as given by
High-pos target.
HP Frames (HpT): Set the HP screen frames for strong positive specimen as given by
High-pos target.
Mixing: Set the number of times the sample is mixed.
Dilution: Setting is reserved until diluted samples are used for microscopy. It is
recommended to use native urine and leave the factor set to 1.
Centrifuge: Setting is reserved until centrifuged samples are used for microscopy. It is
recommended to use native urine and leave the factor set to 1.
Operation:
1. Enter appropriate data into the corresponding box in the Microscopy Setting screen.
2. Click Save after entries are done.
3. Click Quit to quit the Microscopy Setting screen.
3.3.3 Channel Setting
Select Channel Setting to open the Channel Setting screen.
CAUTION: Only authorized technicians can modify channel parameters,
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since the modification affects the test results directly!
Fig. 3-8 Channel Setting screen
Channel Setting Screen Instructions:
Sample: Set the aspiration volume of the sample.
Channel: Set the sample volume filling the Counting Chamber.
Mixing in: Set the mixed sample aspiration volume.
Mixing out: Set the mixed sample drained away volume.
Wash: Set the washing volume of cleanser A for a single wash.
Wash +: Set the washing volume of cleanser A for a single reverse wash.
General: Set the washing volume of cleanser A for a single wash of the sampling needle
outer wall.
Maintain: Set the volume of maintenance cleanser for a single maintenance.
Sample Time: Set the sample time of the peristaltic pump.
Wash Time: Set the wash time of the peristaltic pump.
LP coefficient: Set the LP coefficient for each channel. It has been set in the factory and
is not recommended for change.
Dirty spot: Set the dirty spot number for each channel. It can be set manually or through
Blank Calibration automatically (See Section 7.4 Blank Calibration for details).
Close CH: Choose to close the channel that may have some problems, especially when a
channel of counting chamber is broken.
Operations:
1. Enter appropriate data into the corresponding box in Channel Setting screen.
2. Check the channel for open or close.
3. Click Save after entry.
4. Click Quit to exit the screen.
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3.3.4 Image Setting
Select Image Setting to open the Image Setting screen.
CAUTION: Only authorized technicians can modify channel parameters,
since the modification affects the test results directly!
Fig.3-9 Image Setting screen
Operations:
1. Enter appropriate data into the corresponding box in the Image Setting screen.
2. Drop down the Format box to select image format.
3. Click Save after entry.
4. Click Quit to exit the screen.
3.3.5 Microscope Setting
Fig.3-10 Microscope setting screen
Select Microscope to open the Microscope screen.
CAUTION: All items except Focus Protect have been set in the factory, no
modification is recommended, any change may affect the test results directly, only
authorized technicians can modify channel parameters!
Focus Protect is to set the interval time between the Focus lamps shut in standby mode,
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the default is 30 min., and the time can be set shorter to extend the lamp life.
Operations:
1. Enter appropriate data into the corresponding box in Microscope Setting screen.
2. Click Save after entry.
3. Click Quit to exit the screen.
3.3.6 Morphology Setting
Select Morphology Setting to open the Morphology Setting screen.
CAUTION: Only authorized technicians should modify morphology
parameters, since the modification affects the test results directly!
Fig. 3-11 Morphology Setting screen
Operations:
1. Enter appropriate data into the corresponding Curve box in Morphology Setting
screen.
2. Select specific X Curve and Y Curve in the Scattergraph area.
3. Check the items need to be printed in the Print area.
4. Click Save after setting.
5. Click Quit to exit the screen.
3.4 Operator Management
Operator Management can provide various role authority management and distribution
and ensure the parameters of the software run properly through assigning rights to
different level users.
Click Management - Operator Management to open the Operator Management screen.
Click the “>/<” button in the middle of the screen to assign role rights. Click OK for
confirming. Click Cancel to quit.
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Fig. 3-12 Operator Management screen
CAUTION: If a submenu in “Management” is assigned to the role, it should
be guaranteed that “Management” has been assigned to same role; otherwise the
submenu cannot be displayed and used.
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4 AVE-764 ANALYZER
4.1 Components
The AVE-764 mainly consists of an analyzer, PC peripherals (monitor, mouse and
keyboard) and other attachments (printer for example).
The analyzer is composed of Microscope Module, Pump & Valve System Module,
Sampler Module, Automatic sampler and PC Analysis Processing Modules, also includes
Power System, Barcode Reader and Cooling Fans etc. auxiliary devices.
Fig. 4-1 Front view of the analyzer
Key to figure 4-1
1. Automatic sampler;
2. Microscope observation window;
3. Start button;
4. Reset button;
5. Power LED;
6. Harddisk LED;
7. Barcode Reader
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Fig.4-2 Back view of the analyzer
Key to figure 4-2
1. Cooling fans;
2. COM7, Backup COM port;
3. COM8, Bridge connection port;
4. PC communication ports;
5. Main power input (fuse inside);
6. Waste COM port;
7. Reagent COM port;
8. Cleanser A connector;
9. Maintenance Cleanser connector;
10. Cleanser B connector;
11. Waste connector
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Fig. 4-3 Shaft side view of the analyzer
Key to figure 4-3
1. Main power switch;
2. Tested area;
3. STAT area;
4. Ready area
Fig. 4-4 Front view of the analyzer (front panel removed)
Key to figure 4-4
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1. Sampling module;
2. Magnetic valve pinboard;
3. Pump-valve Main control board;
4. Barcode reader
Fig. 4-5 Back view of the analyzer (back panel removed)
Key to figure 4-5
1. Cooling fan;
2. PC power module;
3. Microscope Module;
4. Main Power module;
5. Wash pool
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Fig. 4-6 Left view of the analyzer (left panel removed)
Key to figure 4-6
1. Main Power module;
2. Pump-valve bracket assembly;
3. Main power switch
Fig. 4-7 Right view of the analyzer (right panel removed)
Key to figure 4-7
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1. Triple solenoid valves assembly;
2. Hard disk;
3. PC motherboard;
4.2 Power System
4.2.1 Components
The power system include one power switch and two power modules; the power modules
located on the rear of the plate, fixed in the middle of instrument (Fig.4-5), one is PC
power module, another is main power module, both are wide input, AC-DC power
modules. The power switch is a green rocker switch on the left of instrument; it’s the main
power switch of the AVE-764.
PC power module is the general computer’s power module, supplying the power for the
computer system of instrument through the standard power connector, and also supplying
power for cooling fans and microscopy lamps with 12 V output;
Main parameters:
Input Voltage: 100-240V~ ,50/60Hz;
Output Power: 400W;
The main power module supplies power for analyzer’s modules through adapter (mainly
include Sample Module, Microscope Module, Automatic Sample Module), excepting
Computer System.
Main parameters:
Input Voltage: 100-240V~ ,50/60Hz;
Output Power: 150W;
Output Voltage: +5V±10%,+24V±10%;
4.2.2 Maintenance and Replacement
CAUTION: The power system is dangerous, be careful of electric shock!
If the power system runs error, firstly, to check the power module has power input or not; If
works properly, disconnect the load cables of power system, then use the multimeter to
measure the power input conforms the norminal voltage or not at the state of power-on. If
not, the power module has to be replaced (Fig. 4-8 to Fig. 4-11).
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Fig. 4-8 PC power module
Key to figure 4-8
1. PC power module;
2. M3X8 Phillips screw
Fig. 4-9 Main Power Module
Key to figure 4-9
1. Main Power Module;
2. M3X8 Phillips screw
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Fig. 4-10 Power Module with mounting plate
Key to figure 4-10
1. M3X6 Phillips screw;
2. Mounting plate;
3. Main Power Module
Fig. 4-11 Power Module Adapter
Key to figure 4-11
1. Power Module Adapter;
2. M3X8 Phillips screw
4.3 Barcode Reader
Barcode Reader (Fig.4-13), located in the middle lower of the instrument.
Precautions:
1.
2.
Applicable type: Code 128, Code 39, Codabar and Interleave 25;
The label must be the standard label paper, the normal printer paper is unavailable;
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4.
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Service Manual
The label should be clicked on the head position of the tube (16x100mm), distance
12.5mm from the tube top. (Refer to the “standard tube”);
Placing the labeled tube on the tube rack and insure the label side right against the
barcode scanner;
If the barcode reader runs abnormally, you could turn off the barcode reader in the
system, under the condition that do not affect the normal operation, see the details in
3.2 Barcode Setting.
Troubleshooting Process:
1.
2.
3.
4.
5.
Checking the barcode reader is open or not in system setting, see details in 3.2
Barcode Setting.
Checking the connection of the barcode reader cable is correct, stable or no loose,
see the connection diagram in 10.1.1 Main Diagram;
Check the barcode reader unlocked or not (barcode reader must be unlocked by
standard barcode on first use);
The serial port tester sends command to check the barcode reader works properly or
not, as showed in Fig.4-12, the command is FB 0C 07. If not, the barcode reader may
be broken, and replacement required.
Fig.4-12 Serial Port Tester Command Screen
Replacing method showed in Fig.4-13.
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Fig. 4-13 Barcode Reader
Key to figure 4-13
1. M3X10 Hexagon screw;
2. Barcode Reader assembly
Fig. 4-14 Barcode Reader Assembly
Key to figure 4-14
1. M3X6 Phillips screw;
2. M3 Flat washer;
3. Reader Box;
4. Barcode Reader
4.4 Cooling Fans & Dust Cover
There are two cooling fans at the rear of analyzer, both draw air into the instrument; one is
built in PC power module, mainly cool the PC power module and left side of the instrument
internal space, another fan is to cool the both sides of instrument internal space. Both of
two cooling fans are powered by PC power module with DC +12V.
The cooling fans are all equipped with dust covers to prevent the dust entering the
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instrument.
CAUTION: To keep good ventilation, suggest to clean the dust covers once
a month.
The removing of the dust covers showed as Fig.4-15. Washing the removed covers with
water, and put it back after drying.
If the running speed of the fans is too low, or the fans no turn in the power-good condition,
the replacement will be required. See Fig.4-16.
Fig. 4-15 Dust Cover
Fig. 4-16 Cooling Fan Assembly
Key to figure 4-9
1. Type-I M4 nuts;
2. Φ4 ring;
3.Cooling Fan;
4. Guard grill;
5. M4X35 Phillips screw
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4.5 COM Board
The COM board is at the rear of the main unit, it provides two ports –COM7(DB9), Backup
COM port; COM8(DB15), Bridge connection port. COM7 is a standard RS-232 serial port,
and COM8 is used to connect urine analyzer or bridge(Uritrail etc).
Fig. 4-17 COM Board Assembly
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5 AUTOMATIC SAMPLER
5.1 Summary
Automatic sampler, located on the front of the AVE-764, it communication with host
computer through serial port; automatic sampler can transfer automatically the sample
racks from the ready area on the right side to the pipetting station and then transport the
racks to the tested area on the left after sampling.
Sample racks, each capable of holding 10 tubes, are loaded on the right side of the
sampler. It will transfer the first tube on the rack to the pipetting station if the operator
presses the “Start microscopy” button on the main screen after start-up. The test tube
location moves after sampling in turn and the sampler will transport the rack to the tested
area after all the samples are taken. This module contains several photoelectric sensors
in it for detecting tube and rack and positioning motor.
Autoloader also has the STAT function as shown in Fig.5-1. In the middle of it located an
STAT module. When there is an STAT sample to be tested, just place the corresponding
tube in the STAT hole and push it to the front and a prompt dialogue box will pop up from
the upper computer. Enter the corresponding information according to the prompt and the
instrument will run the STAT test automatically.
Fig. 5-1 Front view of automatic sampler
5.2 Component
5.2.1 Function areas
The automatic sampler is divided into four functional areas:
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Fig. 5-2 Function area diagram of automatic sampler
5.2.2 Electrical control system
The automatic sampler mainly consists of the follow electrical components:
1. Main control board: Control module is the micro processing center of the module,
responsible for the processing of every I/O signal and functions as the control center
of information exchange with the host computer.
2. Reflective photoelectric sensor: There are lacated at different position for the
running track of rack, It is used for the detection of sample racks or sample tube
photoelectric sensor. There are all together 7 sensors in this module, and the serial
number are: JR1、JR2、JR3、JR4、JR5、JR6、JR8. The functions are listed below:
3.
Stepping motor: Stepping motor is the actuator for transporting sample racks, There
are 4 motors in this module, and the serial number are: MJ1、MJ2、MJ3、MJ4. The
4.
5.
6.
functions are listed below:
Inching photoelectric sensor: It is used for locating the original position of the motor.
There are 4 sensors in this module. and the serial number are:JS1, JS2, JS3, JS4.
The functions are listed below:
Correlation photoelectric sensor: It is used for judging whether there is any STAT
sample or whether the sample rack has been imported. There are 2 sensor in this
module, and the serial number are: JF1, JF2. The functions are listed below:
Power switch: The power supply switch of the automatic sampler.
5.2.3 Electric layout
The Main control board (03A) connected with electric components of the automatic
sample as shown below.
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Fig. 5-3 Main control board (03A) of Automatic Sampler
The inner electric layout of the automatic sampler is shown below.
Fig. 5-4 Electrical components layout 1
Key to figure 5-4
No.
Name
Connected to
Main control
1
Power switch
2
STAT detection
photoelectric sensor
3
Rack in position
photoelectric sensor
board:J1
Main control
board:JF2
Main control
board:JR1
57
Function
The power supply switch of the
automatic sampler.
Detect test tube at the STAT
position
Detect whether the tube rack
has been advanced in place
when bridged with urine
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analyzer
4
Rack full detection
photoelectric sensor
Main control
board:JR4
Detecting and alarming when
the tested area is full of tube
racks
Fig. 5-5 Electrical components layout 2
Key to figure 5-5
No.
Name
5
Tube-detection
photoelectric sensor
Connected to
Function
Main control
Detect whether there is some
sample tube in rack in the
position
board:JR8
Main control
6
Push-out position
photoelectric sensor
Main control
7
Tested area
rack-detection
photoelectric sensor
Main control
8
Ready area
rack-detection
photoelectric sensor
9
Push-in position
photoelectric sensor
board:JR6
board:JR5
board:JR2
Main control
board:JR3
58
Detect whether the sample rack
in test area has been
transferred to withdrawing
position
Coordinate with JR4 to detect
whether the rack in tested area
Coordinate with JR1 to detect
whether the rack in ready area.
Determine whether the tube
rack has been advanced to the
test area
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Fig. 5-6 Electrical components layout 3
Key to figure 5-6
No.
10
Name
Connected to
Main control
Push-out zero detection
photoelectric sensor
11
Sample-changing terminal
detection photoelectric sensor
12
Bridge area motor
13
Bridge area rack-detection
photoelectric
board:JS2
Main control
board:JS1
Main control
14
board:MJ4
Main control
board:JF1
Main control board
/
Main control
15
Push-out motor
board:MJ3
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Function
detect the origin
position of the
mechanical parts of the
pushing-out sample
rack in tested area
detect the terminal of
the rack
lateral-moving in test
area
drive mechanical parts
in bridge area, transfer
rack to ready area
detect whether sample
rack move into bridge
area
the micro processing
center of automatic
sampler
Drive mechanical parts
in tested area and push
the tested sample rack
to tested area
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Fig. 5-7 Electrical components layout 3
Key to figure 5-7
No.
Name
16
Bridge area zero detection
photoelectric sensor
17
Push-in zero detection
photoelectric sensor
Connected to
Function
Main control
detect the origin position of
the mechanical parts of the
bridge
board:JS4
Main control
board:JS3
Main control
18
Sample-changing motor
board:MJ2
Main control
19
Push-in motor
board:MJ1
detect the origin position of
the mechanical parts of the
propulsion sample rack in
ready area
Drive mechanical parts in
testing area and move test
tube rack laterally to test
sample one by one
Drive mechanical parts in
ready area and push the test
sample rack to testing area
5.3 Adjustment
5.3.1 Mechanical testing
Power off the device, manual push and pull each motion mechanism, every parts must
move smoothly, the slider without locking phenomenon and the pawl turnover normally;
Adjust the tension of motion mechanism belt, not too tight or too loose; smooth movement
when push and pull the slider;
5.3.2 Reflective photoelectric sensor adjustment
Due to the functional difference, so each photoelectric sensor has difference requirement
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for the detection sensitivity. Every photoelectric sensor has sensitivity adjustment
potentiometer; you can adjust sensitivity through potentiometer.
CAUTION: Sensitivity adjustment: facing the potentiometer, Reduce sensitivity
when adjust it clockwise, Increased sensitivity when adjust it anticlockwise.
As shown in 5-8, Disconnect the automatic sampler with instrument, take the cover away,
then you can adjust the sensitivity of the photoelectric sensor;
Fig. 5-8 removing the cover of sampler
You need confirm the sensitivity strength by the serial command when you adjust the
potentiometer. The description of serial command and corresponding reply is as below.
Before use the command to detect the photoelectric sensor or motor, the automatic
sampler must power on and communication with host computer normally, they
communication through RS-232 serial port protocol, the default port is COM4. The
detection of photoelectric sensor need use the SSCOM32 tool. As shown in 5-9.
CAUTION: You must quit system software when using the SSCOM32 tool.
Configuration of sampler COM ports:
Baud rate: 9600
Data bits: 8
Stop bit: 1
Parity bit: none
Flow control: none
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Fig. 5-9 SSCOM32
Choose COM4 to set the communication parameters in SSCOM 32. Input test command
into string input box, click HexData, then click send, check the hardware motion and the
reply information from SSCOM32, judge the status of each parts of sampler.
Functionality
Command
Response
Automatically
sampler reset
FB 08 03
Run reset action after receive the command,
return reset successful information as FB 08
03 when everything ok. If communication
seems abnormal, check the connecting
communication line which connect
automatically sampler and host computer
has reliable connection.
Check firmware
version
FB 01 FC
Return version number when system
receives this command.
FB E2 00
Put the tube rack near JR1, then the buzzer
sounding, and gradually move the tube rack
away from JR1, when the distance is greater
than or equal to the width of a tube rack, the
buzzer is no longer tweet;
FB E1 00
If no tube rack stand in ready area, then the
buzzer sounding, if a tube rack in ready
area, the buzzer is no longer tweet;
FB E3 00
Put the tube rack near JR3, then the buzzer
sounding, and gradually move the tube rack
away from JR3, when the distance is greater
than or equal to the width of a tube rack, the
Rack in position
photoelectric
sensor JR1
Ready area
rack-detection
photoelectric
sensor JR2
Push-in position
photoelectric
sensor JR3
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Command
Response
buzzer is no longer tweet;
Tube-detection
photoelectric
sensor JR8
Push-out position
photoelectric
sensor JR6
Tested area
rack-detection
photoelectric
sensor JR5
Rack full detection
photoelectric
sensor JR4
Keep this photoelectric sensor on the perfect
position where the sensor should facing the
tube center, otherwise, adjust photoelectric
sensor installation position; and send
command at this condition, the hardware
should response as below:
FB E4 00
Move a tube rack, if a tube(no matter what
color of liquid inside or empty) in front of
JR8, then the buzzer sounding; if no tube in
that position, then the buzzer is no longer
tweet; Attention the detection sensitivity of
this photoelectric sensor, Prevent
undetected and false detection.
FB E5 00
Put the tube rack near JR6, then the buzzer
sounding, and gradually move the tube rack
away from JR6, when the distance is greater
than or equal to the width of a tube rack, the
buzzer is no longer tweet;
FB F3 00
If no tube rack stand in tested area, then the
buzzer sounding, if a tube rack in tested
area, the buzzer is no longer tweet;
FB F2 00
Put the tube rack near JR4, then the buzzer
sounding, and gradually move the tube rack
away from JR4, when the distance is greater
than or equal to the width of a tube rack, the
buzzer is no longer tweet;
5.3.3 Motor testing
As the same of the photoelectric sensor testing, the four motors also can use this way to
test whether is normal or not; Choose COM4 to set the communication parameters in
SSCOM 32. Input test command into string input box, click HexData, then click send,
check the hardware motion and the reply information from SSCOM32, judge the status of
each parts of sampler.
Functionality
Command
Response
Push-in motor test
FB E7 E2
Push-in motor push the tube rack advancing
in position and then back to origin position
(if no tube rack in ready area, the motor
does not operate), if the error occurred
during the process of movement, then the
buzzer sounding.
Sample-changing
FB E8 E3
If the motor at origin position, then it should
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Command
Response
motor test
Push-out motor test
Bridge area motor
test
move to terminal; if the motor not at origin
position, then it should back to origin
position, if the error occurred during the
process of movement, then the buzzer
sounding.
FB E9 E4
Push a tube rack out, then the motor back to
origin position, if the error occurred during
the process of movement, then the buzzer
sounding.
FB F1 EC
If the motor at origin position, then it should
move to terminal; if the motor not at origin
position, then it should back to origin
position, if the error occurred during the
process of movement, then the buzzer
sounding.
Motor troubleshooting:
During the test, if the motor is abnormal, it should investigation as below:
1. Check the main control broad power supply;
2. Check the motor connection line, confirm the poor contact;
3. Check the motor belt, make sure the tightness is ok;
4. Check the photoelectric sensor which together with motor; refer 5.35;
5. Change Main control board;
6. Change breakdown motor;
5.3.4 Correlation photoelectric sensor testing
The automatic sampler has 2 Correlation photoelectric sensors, one is JF2(as shown in
4-4) which in STAT position, other one is JF1(as shown in 4-6) which in bridge area use for
detecting whether sample rack move into bridge area. We can determine whether the
correlation photoelectric sensor is normal or not by using the multimeter measure their
voltage.
Power on the automatic sampler, when the groove of Correlation photoelectric sensor
have been sheltered , use the multimeter measure the 1,2 pin, the voltage should less
than 1V, and measure 1,3 pin, the voltage should be 5V; when no shelter for the
correlation photoelectric sensor, use the multimeter measure the 1,2 pin, the voltage
should greater than 4V, and measure the 1,3 pin, the voltage should be 5V; otherwise, you
need change the correlation photoelectric sensor or check the connection line between
sensor and Main control board.
5.3.5 Inching photoelectric sensor testing
It is used for locating the original position of the motor. We can determine whether the
inching photoelectric sensor is normal or not by multimeter test it.
Power off the automatic sampler, Depress the Spring leaf of this sensor, when the digital
multimeter measure the 3 pin of the sensor and 1 pin of com port, the multimeter should
display ”0”on its screen and sounding ; Loosen the Spring leaf of this sensor, when the
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digital multimeter measure the 3 pin of the sensor and 1 pin of com port, the multimeter
screen should display”--”and no sounding.
The pins of the inching photoelectric sensor are shown in 5-12.
5.4 Maintenance
5.4.1 Daily maintenance of automatic sampler
To make sure the sampler running properly, timely cleaning the sampler is highly
recommended when there are liquid leaked on the sampler.
CAUTION: Immediately power off the sampler and clean it while a whole
rack samples are pouring onto the sampler.
Tools required: Disinfectant/detergent, gauze, and protective gloves.
Procedure:
1. Power off the instrument. Ensure that the maintenance is performed under power
cut-off condition.
2. Moisten gauze with disinfectant/cleanser and wipe the sampler to remove any
deposits.
3. Check the flexibility of pawls. Moisten cotton swabs with disinfectant/cleanser to
clean pawls, remove any deposits.
4. Wipe again using distilled water.
5. Power on the instrument until the sampler is totally dry.
5.4.2 Reflective photoelectric sensor maintenance
Suggest to clear and maintenance the reflective photoelectric sensor monthly.
Maintenance object: 7 reflective photoelectric sensors and Serial number as: JR1, JR2,
JR3, JR4, JR5, JR6, and JR8 as shown in fig. 5-5, 5-6.
Tools required: Disinfectant/detergent, cotton swabs, protective glove
Maintenance Procedures:
1. Power off the instrument, make sure the operation is in shutdown state.
2. Using a cotton swab moistened with absolute alcohol to swab the surface of
photoelectric sensors
3. Swabs dry them by using a dry cotton swab.
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5.5 Replacement
5.5.1 Reflective photoelectric sensor replacement
Fig. 5-10 reflective photoelectric sensor replacement
Replacement procedure (JR1, JR2, JR3, JR4, JR5, JR6, JR8):
1. Power off the instrument and automatic sampler.
2. Disconnect the terminal between the automatic sampler and host computer, remove
sampler away from instrument.
3. Remove the face shield away from the automatic sampler.
4. Find the corresponding photoelectric sensor, disconnect the terminal of it.
5. Loose two screws on PCB.
6. Replace the photoelectric sensor.
7. Reverse the above steps and install photoelectric sensor back.
8. Test the new sensor and make sure it can work normally.
5.5.2 Correlation photoelectric sensor replacement
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Fig. 5-11 Correlation photoelectric sensor replacement
Replacement procedure (JF1, JF2):
1. Power off the instrument and automatic sampler.
2. Disconnect the terminal between the automatic sampler and host computer, remove
sampler away from instrument.
3. Remove the face shield away from the automatic sampler.
4. Find the corresponding photoelectric sensor, disconnect the terminal of it.
5. Loose two screws on PCB.
6. Replace the photoelectric sensor.
7. Reverse the above steps and install photoelectric sensor back.
8. Test the new sensor and make sure it can work normally.
5.5.3 Inching photoelectric sensor replacement
Fig. 5-12 inching photoelectric sensors replacement
Replacement procedure (JS1, JS2, JS3, JS4):
1. Power off the instrument and automatic sampler.
2. Disconnect the terminal between the automatic sampler and host computer, remove
sampler away from instrument.
3. Remove the face shield away from the automatic sampler.
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4.
5.
6.
7.
8.
9.
10.
Service Manual
Remove the clapboard away from the automatic sampler.
Remove waterproof board.
Find the corresponding photoelectric sensor, disconnect the terminal of it.
Loose screws on inching photoelectric sensor.
Replace the inching photoelectric sensor (with connection line).
Reverse the above steps and install photoelectric sensor back.
Test the new sensor and make sure it can work normally.
5.5.4 Main control board replacement
Fig. 5-13 Main control board replacement
Replacement procedure (03A):
1. Power off the instrument and automatic sampler.
2. Disconnect the terminal between the automatic sampler and host computer, remove
sampler away from instrument.
3. Remove the face shield away from the automatic sampler.
4.
Disconnect the terminal of photoelectric sensors (J R1、JR2、JR3、JR4、JR5、JR6、
JR8、JF2).
5.
6.
7.
8.
9.
10.
11.
12.
Remove the two pawls.
Remove the clapboard.
Remove the waterproof board.
Disconnect all terminals on Main control board.
Remove four copper studs which fixed the Main control board.
Replace the Main control board.
Reverse the above steps and install Main control board back.
Test the new board and make sure it can work normally.
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5.5.5 Motor replacement
Fig. 5-14 Motor replacement
Replacement procedure (MJ1, MJ2, MJ3, MJ4):
1. Power off the instrument and automatic sampler.
2. Disconnect the terminal between the automatic sampler and host computer, remove
sampler away from instrument.
3. Remove the face shield away from the automatic sampler.
4.
Disconnect the terminal of photoelectric sensors (J R1、JR2、JR3、JR4、JR5、JR6、
JR8、JF2).
5.
6.
7.
Remove the two pawls.
Remove the clapboard.
Find the corresponding motor and module which this motor belongs to, remove this
module away from automatic sampler.
8. Remove the motor away from module.
9. Replace the motor.
10. Reverse the above steps and install back.
11. Test the new motor and make sure it can work normally.
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6 PUMP & VALVE SYSTEM MODULE
6.1 Summary
Pump & Valve system module’s main function is to take certain amount of sample out of
the tube, transfer it into the microscopy field, after sample detection finished then washes
the pipeline system, in order to prevent cross-contamination. The main process could
divide into followed modules, such as Wash, Rev-wash, Maintain, Spe-wash, Sample, etc.
All the process modules are controlled by system processing center, if needed; orders
from system will assign a module to work automatically.
Fig.6-1 Pump & Valve module pipeline schematic diagram
Wash:
Use cleanser A to wash sampling pipeline, proceed in two phases: sampling needle
inner-wall and out-wall wash. Taking CH 1 as an example, the flow path is as following:
1. Cleanser A comes out of cleanser A package, through connecting pipeline via Valve
DZ9, Cleanser detector, Cleanser pump, Valve DZ10(normally open), Sampling
needle out-wall into Wash pool, and via DZ1(normally open), Waste pump into Waste
container;
2. Cleanser A comes out of Cleanser A package, through connecting pipeline via Valve
DZ9, Cleanser detector, Cleanser pump, Valve DZ10(normally close), then via Valve
DZ32, Counting chamber, Valve DZ31, Sampling needle inner-wall into Wash pool,
and via Valve DZ1(normally open), Waste pump into Waste container.
Rev-wash (Reverse-wash):
Process comes out the same as normal wash process, in order to make sure it is more
clean, to be called enhance wash.
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Maintain:
The analyzer needs maintenance after daily operation. Click Quit icon on Microscopy
interface and select Quit and Maintenance in the pop up dialog to run maintenance wash
automatically. The maintenance wash is similar with reverse wash in flow path, only
except using maintenance cleanser and open Valve DZ8 not DZ9.
Spe-wash (Special-wash):
If stains and spots are found in Counting chamber, select Spe-wash, the process is similar
to Rev-wash only except using Cleanser B and open Valve DZ7 not Valve DZ9.
Sample:
During the Sampling operation, sampling needle will be pulled out of wash pool, get into
the sample tube, then the Valve of selected channel will be open automatically(such as
Valve DZ31 or DZ32), waste pump works, pump in and out three times in order to make
sure the sample is fully mixed, sample in and transfer into Counting chamber via selected
pipeline.
6.2 Components
Pump & valve system is consist of Main control board, Extend controller module
components, Triple valve and wash pool. Extend controller module components are
consist of the three valves on the right-side of Counting chamber and Adapter Board, etc.
6.2.1 Main control board (02A)
The Main control board located on the left side cross-plate (front-side view), open the left
side-plate, pull off all the plugs and unlock all the fixing screws to take off the board and
replace. After replacement, put back all the plugs and screws in reverse, make sure all
plugs and sockets are one to one correspondence and in right direction.
CAUTION: After replacement, users must log in system as service level,
select Restore Factory Parameter in the drop-down menu of Management to rewrite
the former parameter into the new Main control board.
Fig.6-2 Main control board
Key to figure 4-8
1. 3*6 cross pan-head screw;
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2. Main control board
6.2.2 Extend Controller Module Components
The structure of Extend controller module Components shows below.
Fig.6-3 Extend controller module components (Positive axonometric views)
Fig.6-4 Extend controller module components (Opposite axonometric views)
Key to figure 6-3, 6-4
No.
1
2
3
4
Name
DZ9
DZ8
DZ7
Cleanser
Detector
Connect to
Extend control board: 02B-DZ9
Extend control board: 02B-DZ8
Extend control board: 02B-DZ7
Extend control board: 02B-DZ32
5
Cleanser
Pump
Extend control board: 02B-MJ1
6
Waste pump
Extend control board: 02B-MJ2
72
Function
Cleanser A valve
Maintenance cleanser valve
Cleanser B valve
Detect the existence of
Cleanser A.
The motor drives peristaltic
pump rotating to squeeze the
pipeline and pull the cleanser
into the pipeline.
The motor drives peristaltic
pump rotating to squeeze the
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No.
Name
Connect to
7
8
9
10
DZ2
DZ32
DZ42
DZ52
Extend control board: 02B-DZ2
Extend control board: 02B-DZ32
Extend control board: 02B-DZ42
Extend control board: 02B-DZ52
11
Bidirectional
valve 10
Extend control board: 02B-DZ10
12
Bidirectional
valve 1
Extend control board: 02B-DZ1
13
Extend
control board
Main control board: 02A-J7
Function
pipeline to drain out the waste
after cleansing.
Waste valve
Left valve of Channel 1
Left valve of Channel 2
Left valve of Channel 3
Normally open or closed to
change the trend of waste in
pipeline.
Normally open or closed to
change the trend of cleanser in
pipeline.
Control module of pump motors,
valves, the interface adapter for
cleanser detector
6.2.3 Triple Valve Components
The structure of Triple valve components is as shown below.
Fig.6-5 Triple Valve Components
Key to figure 6-5
No.
14
15
16
17
Name
Adapter
Board
DZ31
DZ41
DZ51
Connect to
Main control board: 02E-DZ31
Adapter Board:02E-DZ31
Adapter Board:02E-DZ41
Adapter Board:02E-DZ51
Function
Right valve of Channel 1
Right valve of Channel 1
Right valve of Channel 2
Right valve of Channel 3
6.2.4 Wash pool
The Wash pool as shown in Fig.6-6, located at mid-lower part of the instrument, which is a
hollow container with a drain hole at the bottom and cleansing pipeline on top used for
cleansing sampling needle wall. There are two pairs of electrode used for detection of
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high and low level inside of wash pool. Wash pool is not only used for collecting tested
sample but also cleansing waste, sampling needle out-wall cleansing waste, etc. The
waste flows to waste container through the Wash pool eventually.
Fig.6-6 Wash pool
Key to figure 6-5
1. 3*10 cross countersunk head screw;
2. Wash pool
6.3 Adjustment
Login software, enter the Management—Adjustment interface. In Adjustment interface,
users are able to adjust the parameters of pump & valve components, as shown in
Fig.6-7.
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Fig.6-7 Adjustment interface
6.3.1 Pump & valve testing
1.
Click Valve button in Adjustment interface to enter into Pump & Valve Test interface,
users can test all the valves separately by ticking On/Off options here.
Fig.6-8 Pump & Valve Test interface
2. If it’s needed to test single pump & valve components, select the corresponding
On/Off options to operate, click Start and observe whether the component works
correctly or not. If it’s not working correctly, the reason may be broken or failure in the
controlling circuit. Try to locate where is the problem by using contiguous same-kind
components to test.
Troubleshooting Operations (take Valve DZ8 as example):
1. If DZ8 is not working, exchange the plug of contiguous working component (such as
DZ9) to the socket of DZ8, then select On/off options to test and observe. If it’s
working again after exchange, that means the Valve DZ8 is broken and needed to be
replaced; If it’s still not working, that means the adapter board may have circuit failure,
replace a new adapter board(02B), bring back all the plugs and connectors and
observe; If it’s still not working, try to replace the main controlling board(02A)
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according to 6.2.1 Main control board (02A).
CAUTION: Because Valve CH1, Valve CH2, Valve CH3 controls both left and
right side valves of counting chamber, once users select the On/Off options, valves
on both sides will respond at the same time.
6.3.2 Pump-Valve Calibration
CAUTION: Pump-Valve calibration is recommended at following conditions:
1. Bubbles occur in counting chamber or the amount of reagent is abnormal;
2. After replacement of rinse/waste bath or tubes (including pump-line, valve-line,
etc);
3. Monthly maintenance;
Operate Pump-Valve calibration as following (CH1 as example):
1. Sample-pump Calib: By selecting SPC (1#, 2# and 3# respectively), a dialog box will
pop up as shown in Fig.6-9. Click OK to continue, the new value will be saved
automatically. The reference value for Sample-pump Calib is 4-5.5s, repeat
sample pump calibration till the value meets the reference range.
2.
Fig.6-9 Sample-pump Calib pop-up interface
Wash-pump Calib: By selecting WPC (1#, 2# and 3# respectively), a dialog box will
pop up as shown in Fig.6-10. Click OK to continue, the new value will be saved
automatically. The reference value for Wash-pump Calib is 3.5-5s, repeat wash
pump calibration till the value meets the reference range.
Fig.6-10 Washing-pump Calib pop-up interface
After calibration, operate as following to confirm:
Wash-pump Calib confirmation
1. Use measuring cylinder to prepare 10 ml cleanser A, put the pipeline connect to
cleanser A container into the cylinder;
2. Click Wash button 3 times in Adjustment interface as shown in Fig.6-7, observe
whether there is 1ml cleanser A left or not, if yes, it is confirmed that the instrument
use 3ml cleanser A each time, error limit of the reagent consumption ≤0.4ml.
3. If the reagent consumption>3ml each time, please find “IniConfig.ini” in the
installation directory of D:\AVE, open the file and find “VentPumpZhi” under
“[CommSet]”, reduce the number a little bit; Otherwise, rise the number a little bit.
Click Save button to confirm the changes.
“VentPumpZhi” range is : 3800~4800;
4. Enter the Adjustment interface, click Pump button to redo, after finished save and
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Quit.
5. Repeat Step 1~2 to confirm the reagent consumption is 3ml or not, if not, repeat Step
3~4.
Sample-pump Calib confirmation
1. Prepare 3 measuring tubes filled with 10 ml saline, put them into a rack, transfer the
rack to check-waiting field;
2. Click Samp All button 4 times in Adjustment interface as shown in Fig.6-7, observe
whether there is 4ml left in each tube or not, if yes, it is confirmed that the sample
consumption volume is 1.5ml each time, error limit of sample consumption ≤0.4ml.
3. If the sample consumption>1.5ml each time, please find “IniConfig.ini” in the
installation directory of D:\AVE, open the file and find “SuckPumpZhi” under
“[CommSet]”, reduce the number a little bit; Otherwise, rise the number a little bit.
Click Save button to confirm the changes.
“SuckPumpZhi” range is : 3500~4500;
4. Enter the Adjustment interface, click Pump button to redo, after finished save and
Quit.
5. Repeat Step 1~2 to confirm the sample consumption is 1.5ml or not, if not, repeat
Step 3~4.
Pump-Valve calibration can also be done in another way: Enter Microscopy main
interface as shown in Fig.6-11, click Tools button and select Calibration Functions in the
drop-down menu, system will pop up Calibration Functions interface as shown in Fig.6-12.
This is also for professional use only.
Fig.6-11 Calibration Functions in Microscopy screen
Fig.6-12 Calibration Functions interface
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6.3.3 Blank Calibration
It is used to determine dirty spots number in the Counting Chamber and improve the
precision of the instrument.
Operations can be done on the right side of the Calibration interface, in the “Blank
Calib” field as shown in Fig.6-12.
1. Prepare 3 tubes filled with a blank reagent (0.9% NaCl).
2. Click the Blank button. Blank Calibration will be done automatically.
3. A prompt will appear after finished.
4. Click Save to confirm the certain number of dirty spots. It will be subtracted from each
result randomly. It is recommended using the blank calibration to determine whether
the channels are clean or not. If the dirty spot exceed 5, the channels might need
additional cleaning or replacement.
CAUTION: If the spots number>5, please do Spe-wash and Blank Calibration
again until it meets the range; Otherwise, it’s recommended to replace the
Counting Chamber.
6.4 Maintenance and servicing
6.4.1 Quit and Maintain
CAUTION: After daily operations, it’s strongly recommended to select Quit
and maintain when exit, the instrument needs daily maintenance and cleansing in
case failures in pipeline system.
Fig.6-13 Quit dialog interface
6.4.2 Valve-tube Maintenance
CAUTION: Cause the valve-tube is clamped when the valve is power off, in
case the valve-tube failures or blocking, it’s recommended to maintain regularly.
Operations:
1. Take the valve-tube out of valve; squeeze the deformation spot to bring it back to
shape;
2. It’s recommended to move the valve-tube up or down 2mm regularly in order to
prevent the same spot be clamped too long and observe whether it is broken or not.
3. If the valve-tube is broken, blocked, can’t be cleansing, can’t bring back to shape,
please replace a new one in the same size and specification.
Valve-tube Specifications:
Name
Material
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Spec(inner/outer diameter)
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Single channel valve-tube
Silicon
Φ2mm×Φ4mm
Dual channel valve-tube
Silicon
Φ1.5mm×Φ3mm
6.4.3 Other pipelines Maintenance
CAUTION: The maintenance of other tubes is recommended at following
conditions:
Operations:
1. Tubes are blocked urine scale or other substance;
2. The connecting spot is ageing, losing stretch, leaking, etc.
3. If maintenance of the tubes can’t solve the problem, please replace a new one in the
same size and specification.
Other tubes Specifications:
Name
Material
Spec(inner/outer diameter)
Tubes at both ends of wash pool
Silicon
Φ1mm×Φ3mm
Waste tube(outer)
PU
Φ2.5mm×Φ4mm
Tube(from the bottom of rinse-bath to
Dual-clip valves)
Teflon
Φ2mm×Φ3.2mm
Other tubes
Silicon
Φ2mm×Φ4mm
6.4.4 Counting Chamber Maintenance
CAUTION: If the instrument can’t suck in sample liquid or bubbles appear in
counting chamber, it may cause by channel blocking, please operate as following:
Operations:
1. Enter Adjustment interface, select corresponding channel, click Spe-wash button
and observe can it be cleansed or not, if can’t, please continue Step 2;
2. Take off the counting chamber, connect one end to a injector and put the other end
into cleanser (diluted hydrochloric acid, 84 Disinfectant, detergent solution, etc)
container, repeat suction and pumping certain times, until it’s cleansed;
3. After all, if the counting chamber is till not able to be cleansed, please replace a new
one, see 8.5.2 Replacement of Counting Chamber for details.
6.4.5 Wash pool Maintenance
CAUTION: It’s recommended that washing and cleansing the wash pool
monthly, mainly including wash pool inner-wall, cleanser entrance and waste exit,
in order to prevent the electrode out of function or tube blocking.
Tools required: deionized water, cotton swab, tube brush, safety gloves;
Operation Steps:
1. Make sure the instrument is powered off;
2. Open the front mask, locate the counting chamber, rise the sampling needle;
3. Use tube brush dipped in deionized water to clean wash pool inner-wall;
4. Use cotton swab dipped in deionized water to clean cleanser entrance and waste
exit;
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Put back the sample needle and fold the front mask;
Restart the instrument, log in the system software, enter the Adjustment interface,
click Wash All button 1~2 times to confirm.
6.5 Replacement
6.5.1 Peristaltic-pump tube replacement
CAUTION: Peristaltic-pump tube is a consumable item, it’s recommended to
check and maintain regularly, normally every 6 months. If the tube is ageing, losing
stretch or broken, please replace a new one ASAP.
CAUTION: Peristaltic-pump tube specification: Φ3.2 (inner diameter)*Φ6.4
(outer diameter)*92 (length) mm, make sure the replacement tube is in the same
size and specification.
Biological Warnings: Before replacement, please follow the safety operation
specification and wear personal protective equipment (such as safety clothing,
safety gloves, etc).
Fig.6-14 Side view of Peristaltic Pump
Replacement Steps:
1. As shown in Fig.6-15, open the pump cover anticlockwise;
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Fig.6-15 Open the pump cover
2.
Fig.6-16 Side view of pump (without cover)
Take out both ends of the pump tube from the groove, as shown in Fig.6-17.
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3.
Fig.6-17 Take out both end of the pump tube
Take off the pump tube , as shown in Fig.6-18
4.
Fig.6-18 Take out the pump tube
Replace a new pump tube and connect both ends tight and correctly.
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5.
Fig.6-19 Replace a new pump tube
Bring the new pump tube back and fix both ends to the groove.
6.
Fig.6-20 Bring backs the pump tube and fix the ends
Put back the pump cover.
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Fig.6-21 Put back the cover
CAUTION: In order to ensure the accuracy of sampling and wash
consumption, please redo pump calibration operation, see 6.3.2 Pump-Valve
Calibration for details.
6.5.2 Disassembly diagram of the pumps and valves
It’s not necessary to break up the entire pump-valve bracket in most cases. Open the left
and rear panels for maintenance, if it’s needed, unplug the connecting cable between the
Pump & Valve system module from the chassis first and unplug other pipeline joints at the
same time, then tear down certain components.
Fig.6-22 Disassembly of the Pump & Valve system module
Disassemble Bidirectional valve assembly
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Tear bidirectional valves down from the bracket as shown in Fig. 6-23. Unplug the
JZ1 and JZ10 first, and open the wire slot along the cable trend, cut the cable-ties,
take off the entire component.
Fig.6-23 Disassembly of the bidirectional valve
Next, tear apart the bidirectional valves as shown in Fig. 6-24.
Fig. 6-24 Disassembly of the bidirectional valves
Key to figure 6-5
1. M3*10 Cross pan-head screw
2. M3 Spring washer
3. Bracket
4. Bidirectional valves
3. Pay attention to the different directions and don’t press the cable when assembling.
Assemble it in reverse.
Disassemble One-way valve assembly
1. Tear apart the one-way valve assembly 2 from the bracket as shown in Fig.6-25.
Unplug the DZ2, DZ32, DZ42 and DZ52 first, and open the wire slot along the cable
trend, cut the cable-ties, take off the entire component.
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Fig.6-25 Disassembly of one-way valve assembly 2
Key to figure 6-5
1. M3*10 Cross pan-head screw
2. Bracket
3. One-way valve
2. Next, tear apart the one-way valves as shown in Fig. 6-26.
Fig.6-26 Disassembly of one-way valves
Key to figure 6-5
1. One-way valve
2. Bracket
3. M3*6 Combination pan-head screws
Disassemble One-way diaphragm valve assembly
It’s not necessary to break up the entire entire one-way diaphragm valve assembly from
the Pump & Valve system module. Remove the two M3*6 combination pan-head screws,
unplug the connecting cable. open the wire slot along the cable trend, cut the cable-ties,
take off the entire component as shown in Fig. 6-27.
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Fig. 6-27 Disassembly of one-way diaphragm valves
Key to figure 6-5
1. One-way diaphragm valve
2. M3*6 combination countersunk-head screws
Disassemble Pumps
Fig. 6-28 Disassembly of pumps
Key to figure 6-5
1. Pumps
2. M3*10 Combination screws
3. Bracket
Disassemble Extend control board (02B)
1. Unplug all the connecting cables and remove all the fixing screws as shown in
Fig.6-29. Tear apart all 4 brass stud as shown in Fig.6-30.
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Fig.6-29 Remove all the fixing screws
2.
Fig.6-30 Take off all 4 brass stud and the Extend control board
As shown in Fig.6-29,6-30, take off the Extend control board, replace a new one and
put it back in reverse.
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6.5.3 Triple valve replacement
Fig.6-31 Adapter Board
Key to figure 6-5
1. M3*6 Cross pan-head screw
2. Adapter Board
1. As shown in Fig.6-31, if it’s needed to replace the adapter board, unplug all the
connecting cables, remove all the fixing screws, replace a new board and put back
the cables and screws correspondingly.
Fig. 6-32 Disassembly of the Triple valve
Key to figure 6-5
1. Bracket
2. Valve
3. 3*M6 Cross pan-head screw
2. As shown in Fig.6-32, remove the 4 fixing screws, bring down the entire bracket.
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Fig. 6-33 disassembly of the one-way valve
Key to figure 6-5
1. Valve
2. Bracket
3. M3*6 Cross Combination screws
3. The disassembly process of the Triple valve must be in order: first, tear apart the
entire bracket, and then tear down the valve off separately.
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7 SAMPLING MODULE
7.1 Summary
The Sampling module is located in front of the instrument; it consists of sampling control
board (02C), sampling pinboard (02D), sampling needle motor and linear guide rail. When
the Sampling module receive the instruction from the analysis processor, the sampling
needle will take in a certain amount of sample after mixed from the test tube, and transfer
it to the field of the microscope. This Sampling module has function of conductivity
detection, anti-collision and liquid level detection.
7.2 Components
The Components of the Sampling module are as shown in 7-1.
Fig. 7-1 Sampling module
The electric component of Sampling module as below:
Key to figure 7-1
NO.
Name
Connect to
Function
1
Sampling
control board
02C
Pump valve main control board:
02A-J12
Sampling motor drive circuit
2
FFC flat cable
Sampling control board: 02C-J6
The connecting cable of
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Name
Connect to
Function
Sampling control board: 02D-J7
control board and pinboard.
Sampling control board:02C-J6
The interface adapter of
Sampling arm
3
Sampling
pinboard 02D
4
Up-down
photoelectric
sensor
Sampling control board: 02D-J3
Locate the origin position
when the sampling arm
moves up and down
5
Up-down
motor
Sampling control board: 02D-J2
Drive the Sampling arm to
move up and down
6
Sampling
needle
photoelectric
sensor
Sampling control board: 02D-J1
Locate the origin position of
the sampling needle
7
Back-forth
motor
Sampling control board: 02C-J7
Drive the sampling arm to
move back and forth
8
Back-forth
photoelectric
sensor
Sampling control board: 02C-J8
Locate the origin position
when the sampling arm
moves back and forth
Sampling control board: 02D-J6
Draw in the sample from the
tube and has functions of anti
collision, conductivity
detection and liquid level
detection.
9
Sampling
needle
7.3 Adjustment
1.
Login the software, enter Management-Adjustment, click ↓ button beside Sample
button, enter Sampling module setting interface, as shown below.
Fig. 7-2 Sampling module adjustment interface
2. Prepare 3 tubes of physiological saline, placed in tube rack.
3. Load the tube rack on ready area, click Sample button, observe the position of the
sampling needle, and go to step 4 to adjust the needle according the criterion below.
Criterion for the Sampling module adjustment
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Project
Features
Criterion
Pool->Sample
The distance of sampling
needle from wash pool to
normal sampling position
Sampling needle should be at the
center of the tube
Pool->STAT
The distance of sampling
needle from wash pool to
SATA position
Sampling needle should be at the
center of SATA position
Bottom of tube
The position of sampling
needle insert into the tube
Sampling needle distance 5mm
from bottom of the tube
Bottom of pool
The position of sampling
needle insert into wash
pool
Sampling needle distance 1mm
from bottom of the wash pool
High level position
Sampling needle stopping
detect liquid level position
The tip of Sampling needle is away
from nozzle of tube 4cm
F-B motor overshoot
The overshoot when the
F-B motor reachs to zero
point
Fixed as 3.8mm
F-B motor speed
The speed of the F-B motor
Fixed as 2
U-D motor
overshoot
The overshoot when the
U-D motor reachs zero
point
Fixed as 3.8mm
U-D motor speed
The speed of the U-D motor
Fixed as 2
The pump run certain time
to drain waste from pipeline
internally
The time should be sufficient to
drain waste
The pump run certain time
to drain Cleanser A from
pipeline internally
The time should be sufficient to
drain Cleanser A
The pump run certain time
to drain Cleanser A from
washing pool (sampling
needle outer)
The time should be sufficient to
drain Cleanser A
The pump run certain time
to aspirate Cleanser B to
counting chamber
The time should be sufficient to
aspirate Cleanser B
The pump run certain time
to drain Cleanser B from
pipeline internally
The time should be sufficient to
drain Cleanser B
Drain waste time
(inner)
Drain Cleanser A
time (inner)
Drain Cleanser A
time (outer)
Aspirate Cleansen B
time
Drain Cleanser B
time
4.
5.
Based on the actual situation, input the proper parameter into input box which
corresponding adjustment item. Click Test button, then the sampling arm will perform
operations after receive the instruction, observer its situation and if necessary, repeat
adjusting until fulfill the adjustment criterion.
Click Save button after adjustment.
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If no response from the Sampling module, see 7.5 Replacement to replace it if
necessary.
7.4 Maintenance
Dredge the sampling needle
If the sampling needle is blocked, following the below steps to dredge the sampling
needle.
1 Make sure the analyzer is in shutdown state.
2 Open the front cover to access the waste pool. Manually lift the sampling needle out.
3 Remove the pipelines from the top of needle, use the pricker thread through from top
of the needle and exit out from the end, clean the dirty stick on the pricker;
4 Repeat step3 several times until there is no dirty stick on.
5 Connect the pipeline back to the needle. Move the needle back to the waste pool and
close the front panel.
6 Restart the instrument and login the software, go to Adjustment interface and click
Wash All button several times to perform channels washing.
7.5 Replacement
CAUTION: Components replacement only can be done in shutdown state.
7.5.1 Replacing sampling control board (02C)
Fig. 7-3 Sampling control board
Key to figure 7-3
1. M3X6 Screw
2. Sampling control board
1. As shown in Fig. 7-3, unplug the cable plug, and loosen the screws, then replace
sampling control board.
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7.5.2 Replacing sampling pinboard (02D)
Fig. 7-4 Sampling pinboard
Key to figure 7-4
1. M3X6 Screw
2. Sampling pinboard
3. the fixed board
4. M3X6 Screw
1. As shown in fig. 7-4, loosen the screws on the fixed board first, then loosen the
screws on sampling pinboard, and replace the sampling pinboard.
7.5.3 Replacing back-forth photoelectric sensor
Fig. 7-5 Back-forth photoelectric sensor
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Key to figure 7-5
1. back-forth photoelectric sensor
2. M2.5X6 Screw
1. As shown in fig. 7-5, loosen the screw, unplug the sensor plug, then replace the
back-forth photoelectric sensor. Login the software; see 7.3 Adjustment to adjust the
sampling needle to proper position.
7.5.4 Replacing up-down photoelectric sensor
Fig. 7-6 Up-down photoelectric sensor
Key to figure 7-4
1. M2.5X6 Screw
2. up-down photoelectric sensor
1. As shown in 7-6, loosens the screw, unplug the sensor plug, then replace the
up-down photoelectric sensor. After the replacement, login the software; see 7.3
Adjustment to adjust the sampling needle to proper position.
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7.5.5 Replacing motors
Fig. 7-7 Motors
1.
Power off the instrument.
2.
Open the front cover, disconnect the plug all wires connected to Sampling module,
and disconnect the pipelines connected to sampling needle.
3.
Loosen the 6 fixed screws that fix the Sampling module and remove the complete
Sampling module out from the device.
4.
Find the corresponding motor, loose the screws as shown in fig. 7-7.
5.
Replace the new motor.
6.
Install new motors back by repeat the above steps in reverse.
7.
See 7.3 Adjustment to adjust the Sampling module to proper situation.
7.5.6 Replacing sampling needle
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Fig. 7-8 Sampling needle module
Key to figure 7-8
1. Fixing ring
2. Screw
3. Guide rod
4. Spring
5. Sampling needle
6. Light block
7. Mounting module
8. Fixing ring
Fig. 7-9 Sampling needle photoelectric sensor
Key to figure 7-9
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1. Sampling needle photoelectric sensor
2. Screw
3. Sampling needle module
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8 MICROSCOPE MODULE
8.1 Summary
With the aid of automatic controlling system, microscope module of AVE-764 can control
the forward/back, left/right, up/down, movements of the microscope stage to ensure the
testing items locate in the imaging center; control the up/down movements of condenser
to adjust focus; control the auto-switch of HP/LP lens, auto focus of CCD, capturing and
photographing of testing items; transfer all the pictures back to the instrument’s main
controlling system. System will finish the auto recognition, classification, counting.
8.2 Components
Components of microscope module:
1. CCD Camera, controlled by Upper-computer, capture and photograph tested items,
transfer the pictures to system;
2. Counting Chamber, CCD will take pictures after sedimentation, the number of
channel differs from types, including 1 channel type, 2 channels type, 3 channels
type.
3. Microscope main control board, receiving orders from Upper-computer and control all
the movement of the microscope;
4. Motors, controlled by Microscope main control board, drive each mechanism;
5. Sensors, locate the initial position of each mechanism.
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Fig. 8-1 Microscope Module (right side view)
Key to figure 8-1
1. CCD
2. Monocular
3. Objective
4. Counting Chamber
5. Counting-Chamber bracket
6. Microscope lamp (inside the box)
7. Main body
8. Controlling Board
9. Left-right motor of stage
10. Up-down motor sensor (in the box)
11. Lamp cover fixing screw
12. Adjust knob fixing clamp
Fig. 8-2 Microscope Module (left side view)
Key to figure 8-2
1. Objective motor
2. Objective motor sensor
3. Up/down motor of stage
4. Left/right motor sensor
5. Focus lamp motor
6. Focus lamp motor sensor
7. Back/forth motor sensor
8. Stage
9. Back/forth motor of stage
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10. Belt
8.3 Microscope and Image Adjustment
Log in the software as service level; select Adjustment-Management to enter the
Adjustment interface.
Fig. 8-3 Adjustment screen
8.3.1 Preparation for microscope focus adjustment
1.
2.
3.
4.
5.
Prepare 3 tubes of RBCs suspensions; put the tubes in a rack.
Load the rack onto the ready area of the sampler.
Select CH1 in Adjustment interface, click MechRS button to run mechanical reset.
Click Samp All button, system will transfer the rack from the ready area to the testing
area and run sampling operation separately.
Wait for about 2 minutes till the RBCs fully settled in the counting chamber. Until now,
the preparation is done and ready to next operation.
8.3.2 Objective Adjustment
CAUTION: The rotation parameter of Objective is very important, if users
need to modify it, please contact AVE service engineer.
1. Click LP Reset button in the function keys field to ensure the vision-field is under LP;
2. Select Lens in the top-left corner of the interface;
3. Click Reset button below the Step box to reset the objective, after finished, try to
rotate the objective, if it’s stuck, that means the objective fit in the “V” groove; if the
objective needs to be rotated a little bit to fit in, that means further adjustment is
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5.
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needed.
Further adjustment is that rotate the objective according to hand feel, type in certain
steps in the step box, click Reset button and observe, for example, going left click and going right click +.
Repeat step 3~4 until the objective fit in the “V” groove. Then click Save LP Mark
button.
Click HP Reset button in the function keys field, set the HP Lens number to “LP
Lens+680” and click Save HP Mark button.
CAUTION: Lens parameter won’t differ from CHs, it’s only necessary to
set the Lens parameter under CH1 LP/HP, which will automatically apply for
CH2, CH3.
8.3.3 Square-Mark Adjustment of Microscope
1.
Square-mark is a scale-plate build in each channel of counting chamber, the position
of the square-mark determines the initial position of image capturing under LP/HP.
Square-mark adjustment reference range under LP/HP as follows:
Lens
Left-Right
F-Backward
Up-Down
Lens
Focus
lamp
LP
400~800
600~1000
700~1600
50~250
700
HP
HP-LP≤120
HP-LP≤120
HP=LP
700~850
200
LP
400~800
CH2-CH1=2830
700~1600
50~250
700
HP
HP-LP≤120
HP-LP≤120
HP=LP
700~850
200
LP
400~800
CH2-CH1=2830
700~1600
50~250
700
HP
HP-LP≤120
HP-LP≤120
HP=LP
700~850
200
CH
CH1
CH2
CH3
2.
Factory parameters will be provided with counting chamber, after replacement, enter
Management-Adjustment, click Replace chamber button, type in the new
parameters provided with new counting chamber, click Save button and Quit.
Fig.8-5 Replace chamber window
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4.
5.
6.
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After parameter modification, the instrument will calibrate the parameters of counting
chamber and stage position automatically correspondingly and check whether the
square-mark position and focus achieved best condition or not; If not, please adjust
microscope according to following operation steps to achieve best state:
Select CH 1 in Adjustment interface, click LP Reset button to ensure LP field;
Select F-Backward, type in certain steps in the step box(such as 50), click “+” or “-”
button to modify the step to 550;
Select Left-right, change the step to 400 according to step 5, type in 50 into the step
box and click “+” button until the square mark appears;
Select F-backward or Left-right, decrease the step typed in the step box orderly (50,
30, 20, 10, 5, etc), only until the square-mark locates as shown in Fig.8-6, then click
Save LP Mark button.
8.
Fig.8-6 LP square-mark position
Click HP Reset button to ensure HP field, repeat step 5~7 to locate HP square-mark
as shown in Fig.8-7 and click Save HP Mark button to confirm.
9.
Fig.8-7 HP square-mark position
Complete square-mark position adjustment for CH2, CH3 by repeat step 4~8, only
except the F-Backward step should be 3385(CH2) and 6220(CH3) in step5.
8.3.4 Focus Adjustment
After the adjustment of square-mark position, it’s necessary to adjust the focus, operation
as follows:
Procedure of Auto-Focus:
1. Prepare 3 tubes of RBC suspensions, put the tubes in a rack and load the rack in
ready area of sampler;
2. Click Samp All button, wait until finished, select CH1, click Auto-Focus and observe
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HP image, system will complete focus adjustment automatically;
3. Click Save in the pop up window to confirm.
4. Then check the foucus of CH2 and CH3.
5. If the result of Auto-Focus is not desired, continue with Manual-Focus as follows:
Procedure of Manual-Focus
1. Select “CH1” then click LP Reset.
2.
3.
4.
5.
6.
7.
8.
Check the
, and enter 5-10 step in “Step”, click “-” or “+” to adjust the
image to the most clear state (compare with the standard gallery).
Click “Save LP Mark” to save.
Click HP Reset;
Check the
, and enter 2-5 step in “Step”, click “-” or “+” to adjust the
image to the most clear state (compare with the standard gallery).
Remember the HP
value, then click LP Reset, and enter certain
value in “Step” to adjust the LP
value same as HP value.
Then click “Save LP Mark” to save.
Perform the same procedure to calibrate the CH2 and CH3.
8.3.5 Image Adjustment
1.
Click Image button in the Adjustment interface as shown in Fig.8-8. Users can set
the background brightness, gray level, color gain, etc.
Fig.8-8 Image setting window
Reference range for parameters:
Gray: 12~16, 12 as default;
Gain: Green: 15~25; The Soft Gain Picture of Brightness, Red, Green, Blue should
be basically the same to Ref value, difference should not be larger than 10;
2. Click Image button in Adjustment interface, select LP in Precise Adjust field;
3. Click AE On and AWB On button one by one, system will adjust automatically.
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4.
5.
Type in Bright/Gray (10) and Gain (1) in Precise Adjust field.
Click the L+ or L- button in Brightness field to adjust the image exposure value as
same as the reference value. Click Save Exposure to confirm.
6. Click R+/R-, G+/G- and B+/B- buttons to adjust the image gain value to the
reference values (difference less than 10). Click Save Gain button to confirm.
7. Click Save All after all finished.
Select HP in Precise Adjust field, finish the adjustment in the same way as above. Click
Save All button to confirm.
8.4 Maintenance
8.4.1 Cleaning of Counting-chamber and stage
Tools required: 95% ethanol, absorbent cotton swab, no-dust cloth;
Operation Steps:
1. Turn off the instrument, Open the front mask, use cotton swab dipped in ethanol to
clean the surface of chamber;
2. Use cloth dipped in ethanol to clean the stage;
3. Use cotton swabs to wipe until dry;
CAUTION: Do NOT wipe forth and backward, please wipe in one direction.
8.4.2 Cleaning of Sensors
Tools required: 95% ethanol, absorbent cotton swab;
Operation Steps:
1. Turn off the instrument, make sure the power is off;
2. Open the front mask and left side panel;
3. Use cotton swab dipped in ethanol to clean the sensors;
4. Use cotton swabs to wipe until dry;
8.4.3 Cleaning of Micro-lamp and Focus lamp
Tools required: 95% ethanol, absorbent cotton swab, no-dust cloth;
Operation Steps:
1. Turn off the instrument, make sure the power is off;
2. Open the front mask;
3. Use cloth dipped in ethanol to clean the Focus lamp;
4. Use cloth dipped in ethanol to clean the micro-lamp basement;
5. Use cotton swabs to wipe until dry;
CAUTION: Clean the micro-lamp after cooling in case burning.
8.4.4 Maintenance of rotation axis
Tools required: 95% ethanol, absorbent cotton swab, lubricating oil;
Operation Steps:
1. Turn off the instrument, make sure the power is off;
2. Open the front mask and left side panel;
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Use cotton swab dipped in ethanol to clean the rotation axis;
Wait till dry, use cotton to lubricate the rotation axis;
8.4.5 Maintenance of counting chamber
1.
2.
3.
4.
Maintenance is needed if dirty spot or blocking found in counting chamber, operate as
follows.
Select corresponding channel in Adjustment interface, click Spe-wash and observer
whether it’s cleaned or not, if not, continue Step 3;
Take off the counting chamber, connect one end to a injector and put the other end
into cleanser(diluted hydrochloric acid, 84 Disinfectant, detergent solution, etc)
container, repeat suction and pumping certain times, until it’s cleansed;
If still can’t be cleaned, please replace a new one.
8.5 Replacement
8.5.1 Replacement of Micro-lamp
Micro-lamp is a consumable item, affected by external environment (such as Voltage,
Temperature, Humidity, etc) or using frequency, leading to out of brightness or broken,
please replace it based on actual condition.
CAUTION: Replacement is recommended when micro-lamp is out/lack of
brightness, dark/black images appear, recognition is affected.
CAUTION: Specification of micro-lamp: 12V, 20W.
CAUTION: Replace the micro-lamp after power off and cooling, replace
with one in same size and specification.
CAUTION: Replace the micro-lamp after cooling in case burning.
Operation steps of replacing the micro-lamp:
1. Power off, make sure the micro-lamp is completely cooled, open the front mask,
locate the microscope and the hand-screw on lamp box as shown in Fig.8-9;
2. Unlock the hand-screw, open the micro-lamp box cover, as shown in Fig.8-10;
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Fig.8-9 Hand-screw on micro-lamp box
3.
Fig.8-10 Open the micro-lamp box
Remove the lamp-shade, as shown in Fig.8-11.
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Fig.8-11 Micro-lamp
Take off the lamp, replace a new one, put back in reverse;
CAUTION: If the new micro-lamp can’t be light, please take off the lam
and plug in again, the problem is caused by poor contact randomly.
8.5.2 Replacement of Counting Chamber
CAUTION: Counting Chamber is a consumable item, affected by external
environment or using frequency, leading to dirty spot, scratches or broken,
please replace according to actual condition.
CAUTION: If dirty spots or scratcher are in large quantity and can’t be
removed, affecting recognition, please replace a new one after power-off and
cooling.
Operation steps of replacing counting-chamber:
1. Take out the new counting-chamber, double confirm whether the serial number on
chamber and the package are the same or not;
2. Open the front mask, locate the counting-chamber, as shown in Fig.8-12;
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Fig.8-12 Counting-chamber on the stage
Turn the lens a little bit, rise the clips and release the counting-chamber with bracket,
disconnect the pipelines and bring out the entire unit, as shown in Fig.8-13;
Fig.8-13 Bring out the counting-chamber with bracket
Unlock the screws, separate the counting-chamber, as shown in Fig.8-14;
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Fig.8-14 separates the counting-chamber and bracket
Replace a new chamber, connect the pipelines correspondingly, as shown in Fig.8-15
CAUTION: The installation direction of counting-chamber is that convex-side
up and the broadside with serial numbers face inside; The pipeline must connect
color to color and cover at lease 1/2 of the connector surface.
6.
Fig.8-15 Replace a new counting-chamber
Put back the new chamber into bracket after pipelines connected, bring back the
screws and fix the new chamber in the bracket, as shown in Fig.8-16.
CAUTION: Install the new counting-chamber back into the bracket,
upper-right corner against the bracket; The chamber tabletting should press to the
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chamber edge from 1-2 mm.
Fig.8-16 Fixing the new chamber in bracket
7. Push the bracket back under the tabletting. Make sure the positioning balls just drop
into the fixed holes.
8. Close the front panel after finished.
9. Input the parameters of new counting chamber.
After replacing the counting chamber, the parameters must be modified in the
software. Log in software, enter Management-Adjustment, click Replace Chamber
button to pop up the Replace chamber interface, as shown in Fig.8-17, type in the
parameters of new chamber (provide with chamber package box). Click Save button
to confirm and Quit button to exit.
10. Focus Confirmation
After parameters modification, microscope system will calibrate based on new
parameter automatically. Check focus and square-mark position under LP/HP of each
channel. If the condition is not satisfied, refer to 8.3 Microscope and Image
Adjustment for further operations.
11. Calibration and Verification of chamber coefficient
Launch QC process to calibrate and verify the coefficient of new chamber, make sure
the focus is correct and working before QC. If the instrument is within QC control, it
means the coefficient is correct, if the instrument is out of QC control, it means the
coefficient needs to be calibrating on premise of correct and working instrument.
12. Finish QC by using the QC liquid, repeat 10 time each channel, count the item
quantity of 100 frames and divide by 100 to get the average quantity per frame.
Chamber coefficient=QC Concentration/average quantity per frame.
CAUTION: The reference range of chamber coefficient:15.5~19.5, if the
coefficient is out of range, please contact AVE service engineer.
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8.5.3 Replacement of CCD
Fig.8-18 CCD Installation
Key to figure 8-18
1. CCD
2. CCD cable fixing screw
3. CCD USB interface
4. C flex
5. CCD connector
6. Hand screw
CAUTION: Power off the instrument before replacing CCD.
CCD replacement operation steps:
1. Use cross screw-driver to unlock the CCD cable fixing screws;
2. Take off the CCD USB cable;
3. Unlock the hand screw(by hand or by tools if needed) until the entire CCD component
can be pull out(Fig.8-18 1,4,5);
4. Bring out the new CCD, remove the package and cover;
CAUTION: During the installation of CCD, the camera lens must face down,
Do not blow to CCD camera lens field in case dirty spots.
5. Install the new CCD in reverse, pay attention to the installation direction(USB
interface facing backward), make sure the connection is fixed and tight;
6. Bring back the CCD USB cable and fixing screws;
7. Power on the instrument, check whether the square-mark frame is parallel with the
vision field or not, if not, adjust the CCD position and fix the hand screw tightly;
8. After replacement, check the focus and square-mark position under HP of each
channel, if the focus is unexpected, please refer to 8.3 Microscope and Image
Adjustment to complete the adjustment, make sure focus is in best condition.
CAUTION: If dirty spots on CCD is found, please contact AVE service
engineer.
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8.5.4 Replacement of Sensors
CAUTION: Power off the instrument before replacing the sensors.
All sensors on microscope are correlation type, mainly used for locating the initial position
of motors, named after the corresponding position.
1. Replacing objective motor sensor
Fig.8-19 Objective motor sensor
Key to figure 8-19
1.I type nut M2.5
2.φ2.5 spring washer
3.φ2.5flat washer
4. Objective motor sensor
5. M2.5X6cross pan-head screw
As shown in Fig.8-19, tearing-down starts with the fixing screws, unlock and take off the
sensor, unplug the cables, replace a new sensor, put it back and fix.
2. Replacing Left/right motor sensor
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Fig.8-20 Left/right motor sensor
Key to figure 8-20
1. Holder;
2. M3 flat-washer;
3. M3 spring-washer;
4. M3*8 cross recess pan-head screw;
5. Left/right motor sensor ;
6. M2.5*6 cross pan-head screw;
As shown in Fig.8-20, tearing-down starts with the fixing screws, unlock and take off the
sensor, unplug the cables, replace a new sensor, put it back and fix.
3. Replacing Focus lamp motor sensor
Fig.8-21 Focus lamp motor sensor
Key to figure 8-21
1. M2.5*6 cross pan-head screw
2. Focus lamp motor sensor
As shown in Fig.8-21, tearing-down starts with the fixing screws, unlock and take off the
sensor, unplug the cables, replace a new sensor, put it back and fix.
4. Replacing forth/back motor sensor
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Fig.8-22 Forth/back motor sensor
Key to figure 8-22
1. Forth/back motor sensor
2. M2.5*6 cross pan-head screw
As shown in Fig.8-22, tearing-down starts with the fixing screws, unlock and take off the
sensor, unplug the cables, replace a new sensor, put it back and fix.
5. Replacing up/down motor sensor
Fig.8-23 Up/down motor sensor
Key to figure 8-23
1. M2.5*8 cross pan-head screw
2. M2.5 spring washer
3. M2.5 flat washer
4. Shelter
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5. Narrow-groove sensor
6. M2.5*6 cross pan-head screw
As shown in Fig.8-23, tearing-down starts with the fixing screws, unlock and take off the
sensor, unplug the cables, replace a new sensor, put it back and fix.
8.5.5 Replacement of microscope main control board
Fig. 8-24 Microscope main control board
Key to figure 8-24
1. Main control board
If it’s needed to be replaced, open the rear panel, unplug all the connecting cables,
remove all the fixing screws, replace a new one, put back all the cables and plugs
correspondingly in right direction.
CAUTION: After replacement, it’s necessary to confirm the Lower-computer
program version, the version must be as same as former version. If not, see 10.3
Upgrading the module firmware for details.
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9 TROUBLESHOOTING
9.1 Sampler error message
No.
1
2
Symptom
After starting the
examination, the
system notice as
‘Sampler
communication
timeout’.
There is no rack
loaded at ready
area but the
Pawls keep
pushing forward
and back,
meanwhile the
system notices
as ‘Push-in
motor at ready
area reaching
position error.’
Probable cause
Recommended action
The communication cable
connected with sampler was
loosen or not well connected.
Re-connect the cable.
Check whether the switch
located at left side of sampler is
turn on.
If not, turn on the switch.
The COM port number was not
well defined or was changed.
The default COM port number
of sampler is COM4.
Open a COM tools
(sscom32E.exe) to find current
port communicated with sampler
and if necessary, change it to
COM4. Launch the system and
go to Management-System
Settings-COM setting, check
whether the COM port number of
sampler is COM4.
The control module of sampler
was damaged.
See 5.5.4 Main control board
replacement to replace the
control module.
Check whether the sampler is
placed at a direct strong light
area.
If so, prevent the sampler from
strong light.
There is dirt stick on the surface
of photoelectric sensor (JR1,
JR2).
The sensitivity of JR2 is too low
or was invalid.
The control module of sampler
was damaged.
118
Power off the sampler and Use a
cotton swab moistened with
absolute alcohol to swab the
interface to cleanup.
See 5.3.2 Reflective photoelectric
sensor adjustment to increase
the sensitivity of JR2. If the issue
persists, see 5.5.1 Reflective
photoelectric sensor replacement
to replace JR2.
See 5.5.4 Main control board
replacement to replace the
control module.
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No.
3
4
5
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Service Manual
Symptom
The system
notices as
‘Sensor to
detect racks
loaded at bridge
area error.’
The rack at
testing area can
not be shifted by
two hooks.
Sample was
missed
examination by
instrument.
The empty
position on rack
was mistakenly
identified.
Probable cause
Recommended action
JF1 error.
See 5.3.4 Correlation
photoelectric sensor testing to
test JF1, if JF1 do have problem,
see 5.5.2 Correlation
photoelectric sensor replacement
to replace it.
Motor MJ4 error.
See 5.3.3 Motor testing to test
MJ4, if MJ4 do have problem, see
5.5.5 Motor replacement to
replace it.
The control module of sampler
was damaged.
See 5.5.4 Main control board
replacement to replace the
control module.
Check whether the tube rack is
for specialized use.
Change the tube rack if it is not.
The hooks may be obstructed
by dirt or deposits.
Check the flexibility of hooks.
Moisten cotton swabs with
disinfectant/cleanser to cleanup,
remove any deposits.
The control module of sampler
was damaged.
See 5.5.4 Main control board
replacement to replace the
control module.
The sensitivity of JR8 is too low
or was invalid.
See 5.3.2 Reflective photoelectric
sensor adjustment to increase
the sensitivity of JR8. If the issue
persists, see 5.5.1 Reflective
photoelectric sensor replacement
to replace JR8.
The control module of sampler
was damaged.
See 5.5.4 Main control board
replacement to replace the
control module.
There is dirt stick on the surface
of JR8.
Power off the sampler and Use a
cotton swab moistened with
absolute alcohol to swab the
interface to cleanup.
JR8 was not located right
opposite the middle of test tube
surface.
Remove the cover of sampler to
adjust JR8 to the standard
position.
Check whether the sampler is
placed at a direct strong light
area.
If so, prevent the sampler from
strong light.
The sensitivity of JR8 is too
See 5.3.2 Reflective photoelectric
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Symptom
Probable cause
high.
7
8
9
There is no rack
loaded at tested
area but the
pawls keep
pushing.
Meanwhile
the system
notice as
‘Push-out motor
at tested area
reaching
position error.’
There is no rack
loaded at tested
area or the
tested area is not
full, but the
system notices
as ‘Tested area
is full.’
The system
notices as
‘Loading motor
at bridge area
home position
error.’
Recommended action
sensor adjustment to decrease
the sensitivity of JR8.
The control module of sampler
was damaged.
See 5.5.4 Main control board
replacement to replace the
control module.
Check whether the sampler is
placed at a direct strong light
area.
If so, prevent the sampler from
strong light.
There is dirt stick on the surface
of JR6.
The sensitivity of JR6 is too
high.
Power off the sampler and Use a
cotton swab moistened with
absolute alcohol to swab the
interface to cleanup.
See 5.3.2 Reflective photoelectric
sensor adjustment to decrease
the sensitivity of JR6.
The control module of sampler
was damaged.
See 5.5.4 Main control board
replacement to replace the
control module.
Check whether the sampler is
placed at a direct strong light
area.
If so, prevent the sampler from
strong light.
There is dirt stick on the surface
of JR4.
Power off the sampler and Use a
cotton swab moistened with
absolute alcohol to swab the
interface to cleanup.
The sensitivity of JR4 is too
high.
See 5.3.2 Reflective photoelectric
sensor adjustment to decrease
the sensitivity of JR4.
The control module of sampler
was damaged.
See 5.5.4 Main control board
replacement to replace the
control module.
Check whether the wire of JS4
connected with control module
was loosening. Re-connect it if
necessary.
JS4 error or the control module
was damaged.
See 5.3.5 Inching photoelectric
sensor testing to test JS4.
Replace it if JS4 did have
problem.
See 5.5.4 Main control board
replacement to replace the
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Symptom
Probable cause
Recommended action
control module.
10
11
12
13
The system
notice as
‘Push-in motor
at ready area
home position
error.’
The system
notice as
‘Push-in motor
at ready area
home position
error.’
The system
notice as
‘Push-out motor
at tested area
home position
error.’
The system
notice as
‘Loading motor
at bridge area
mechanical
error.’
Check whether the wire of JS1
connected with control module
was loosening. Re-connect it if
necessary.
JS1 error or the control module
was damaged.
See 5.3.5 Inching photoelectric
sensor testing to test JS1.
Replace it if JS4 did have
problem.
See 5.5.4 Main control board
replacement to replace the
control module.
Check whether the wire of JS2
connected with control module
was loosening. Re-connect it if
necessary.
JS2 error or the control module
was damaged.
See 5.3.5 Inching photoelectric
sensor testing to test JS2.
Replace it if JS4 did have
problem.
See 5.5.4 Main control board
replacement to replace the
control module.
Check whether the wire of JS3
connected with control module
was loosening. Re-connect it if
necessary.
JS3 error or the control module
was damaged.
See 5.3.5 Inching photoelectric
sensor testing to test JS3.
Replace it if JS4 did have
problem.
See 5.5.4 Main control board
replacement to replace the
control module.
The belt of motor (MJ4) is too
tight.
Adjust the belt adhesion to be
within operational limits.
The guide of motor was
damaged.
Replace the guide.
See 5.3.3 Motor testing to
check whether MJ4 was
damaged.
See 5.5.5 Motor replacement to
replace MJ4.
The control module of sampler
See 5.5.4 Main control board
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Symptom
The system
notice as
‘Push-in motor
at ready area
mechanical
error.’
The system
notice as
‘Sample-switchi
ng motor at
testing area
mechanical
error.’
The system
notice as
‘Push-out motor
at tested area
mechanical
error.’
Probable cause
Recommended action
was damaged.
replacement to
control module.
replace
the
The belt of motor (MJ1) is too
tight.
Adjust the belt adhesion to be
within operational limits.
The guide of motor was
damaged.
Replace the guide.
See 5.3.3 Motor testing to
check whether MJ1 was
damaged.
See 5.5.5 Motor replacement to
replace MJ4.
The control module of sampler
was damaged.
See 5.5.4 Main control board
replacement to replace the
control module.
The belt of motor (MJ2) is too
tight.
Adjust the belt adhesion to be
within operational limits.
The guide of motor was
damaged.
Replace the guide.
See 5.3.3 Motor testing to
check whether MJ2 was
damaged.
See 5.5.5 Motor replacement to
replace MJ2.
The control module of sampler
was damaged.
See 5.5.4 Main control board
replacement to replace the
control module.
The belt of motor (MJ3) is too
tight.
Adjust the belt adhesion to be
within operational limits.
The guide of motor was
damaged.
Replace the guide.
See 5.3.3 Motor testing to
check whether MJ3 was
damaged.
See 5.5.5 Motor replacement to
replace MJ3.
The control module of sampler
was damaged.
See 5.5.4 Main control board
replacement to replace the
control module.
9.2 Pump-valve module error message
No.
1
Symptom
There is bubble
existing in the
channel after
inhaling sample
or the sample
can not be
Probable cause
Recommended action
Check whether the
sampling needle was
blocked.
See 7.4 Maintenance to dredge
Sampling needle.
Check whether the counting
chamber was blocked.
See 6.4.4 Counting Chamber
Maintenance for further process.
Check whether there is
See 6.1 Summary to check the
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Service Manual
Symptom
Probable cause
inhaled into the
channel.
The system
notice as
‘Sampling
channel leakage
or blockage.’
broken or leakage pipelines.
Check all the connectors
between two pipelines.
pipelines according the piping
diagram. If any problems are found,
see 6.4.3 Other pipelines
Maintenance for further process.
Check all pinch valve
pipelines to see whether
anyone was broken.
See 6.4.2 Valve-tube Maintenance to
replace the broken pinch valve
pipelines.
Pinch valves error or waste
pump (MJ2) error.
See 6.3.1 Pump & valve testing to
test whether pinch valves or waste
pump (MJ2) have problem. Replace
the error components if necessary.
Pump tube of MJ2 was
broken or aging.
See 6.5.1 Peristaltic-pump tube
replacement to replace the pump
tube.
The parameters related to
sampling are not correct or
changed.
See 6.3.2 Pump-Valve Calibration to
perform sampling pump calibration.
Check whether the
sampling needle was
blocked.
See 7.4 Maintenance to dredge
Sampling needle if necessary.
Maybe one of the connector
located on the pipeline from
sampling needle to the right
end of counting chamber is
broken or disconnected.
See 6.1 Summary to check the
pipelines according the piping
diagram. If any problems are found,
see 6.4.3 Other pipelines
Maintenance for further process.
Check whether cleanser A
was run out.
If so, replace a new one.
Because the instrument was
not use for long time, the
pipelines were filled with air.
Confirm the error message several
times for its own eliminated.
Check whether there is
broken or leakage pipelines.
Check all the connectors
between two pipelines.
See 6.1 Summary to check the
pipelines according the piping
diagram. If any problems are found,
see 6.4.3 Other pipelines
Maintenance for further process.
Maybe one of valves was
not properly open.
See 6.3.1 Pump & valve testing to
test whether pinch valves have
problem. Replace the error
components if necessary.
Pump tube of MJ1 was
broken or aging.
See 6.5.1 Peristaltic-pump tube
replacement to replace the pump
tube.
Though this is a
visible positive
sample, no cells
can be seen on
the screen after
inhaling into the
channel.
The system
notice as ‘No
cleanser A’.
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Recommended action
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No.
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5
6
7
Service Manual
Symptom
The system
notice as ‘No
cleanser B’.
The system
notice as ‘No
maintenance
cleanser’.
The system
notice as
‘Cleaning pool
drain pipe
leakage or
blockage’.
The system
Probable cause
Recommended action
The washing pump (MJ1)
has no action.
See 6.3.1 Pump & valve testing to
test whether washing pump has
problem. Replace the error
components if necessary.
Check whether cleanser B
was run out.
If so, replace a new one.
The pinch valve pipeline of
DZ7 was stuck.
See 6.4.2 Valve-tube Maintenance
for further process.
The valve DZ7 was not
properly open.
See 6.3.1 Pump & valve testing to
test whether DZ7 has problem.
Replace it if necessary.
Check whether
maintenance cleanser was
run out.
If so, replace a new one.
The pinch valve pipeline of
DZ8 was stuck.
See 6.4.2 Valve-tube Maintenance
for further process.
The valve DZ8 was not
properly open.
See 6.3.1 Pump & valve testing to
test whether DZ8 has problem.
Replace it if necessary.
Check whether the waste
pipeline was block or
pressed.
See 6.1 Summary to check the
pipelines according the piping
diagram. If any problems are found,
see 6.4.3 Other pipelines
Maintenance for further process.
Outlet at the bottom of wash
pool was block.
See 6.4.5 Wash pool Maintenance
for further process.
Pump tube of MJ2 was
broken or aging.
See 6.5.1 Peristaltic-pump tube
replacement to replace the pump
tube.
The wire connected
between wash pool with
main control module was
loosen or disconnected.
See 10.1.3 Sampling Module
Diagram to check whether the wire
was loosen. Re-connect it if
necessary.
Sensor detecting upper
level of wash pool was
invalid.
See 6.4.5 Wash pool Maintenance
for further process.
Waste pump (MJ2) has no
action.
See 6.3.1 Pump & valve testing to
test whether MJ2 has problem.
Replace it if necessary.
Main control module was
damaged.
See 6.2.1 Main control board (02A) to
replace it.
Check whether the waste
Discard waste according to
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Symptom
notice as ‘Waste
container is
full’.
8
9
10
11
After inhaling
sample into the
channel, the
sample appears
not be still but
flowing.
Cross-contamina
tion
The system
notice as
‘Reagent
communication
timeout!’
The system
notice as
‘Cleaning pool
overflow’.
Probable cause
Recommended action
container is full.
applicable regulations.
The waste sensor gave a
false alert.
Clean the waste sensor.
The pinch valve pipelines
are aging thus can not be
completely clamped.
See 6.4.2 Valve-tube Maintenance
for further process, replace it if
necessary.
Check whether the pinch
valves on both sides of
counting chamber can not
be properly close.
See 6.3.1 Pump & valve testing to
test whether any valve has problem.
Replace the error components if
necessary.
Check whether the
sampling needle was
blocked.
See 7.4 Maintenance to dredge
Sampling needle.
Check whether the counting
chamber was blocked.
See 6.4.4 Counting Chamber
Maintenance for further process.
Inlet on the top of wash pool
was blocked.
See 6.4.5 Wash pool Maintenance
for further process.
Other pipelines were
blocked.
See 6.1 Summary to check the
pipelines according the piping
diagram. If any problems are found,
see 6.4.3 Other pipelines
Maintenance for further process.
The pump system needs to
be calibrated.
See 6.3.2 Pump-Valve Calibration to
calibrate the pump system.
The pump tubes were
aging.
See 6.3.2 Pump-Valve Calibration to
calibrate the pump system. If
necessary see 6.5.1 Peristaltic-pump
tube replacement to replace the
pump tubes.
The reagent cable was not
well connected or damaged.
Re-connect the cable or replace it.
The reagent sensor was
damaged.
Replace a new sensor.
The extend control module
(02B) was damaged.
Replace the extend control module.
Check whether the waste
pipeline was block or
pressed.
See 6.1 Summary to check the
pipelines according the piping
diagram. If any problems are found,
see 6.4.3 Other pipelines
Maintenance for further process.
Outlet at the bottom of wash
See 6.4.5 Wash pool Maintenance
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Service Manual
Symptom
The system
notice as
‘Reagent Stop’
while perform
pump calibration
in Adjustment
interface.
The system
notice as ‘Pump
System
communication
timeout’.
Probable cause
Recommended action
pool was block.
for further process.
Pump tube of MJ2 was
broken or aging.
See 6.5.1 Peristaltic-pump tube
replacement to replace the pump
tube.
The wire connected
between wash pool with
main control module was
loosen or disconnected.
See 10.1.3 Sampling Module
Diagram to check whether the wire
was loosen. Re-connect it if
necessary.
Waste pump (MJ2) has no
action.
See 6.3.1 Pump & valve testing to
test whether MJ2 has problem.
Replace it if necessary.
Main control module was
damaged.
See 6.2.1 Main control board (02A) to
replace it.
Air was inhaled into the
pipeline.
Re-try several times to eliminate the
alert automatically.
The detection sensitivity of
wash pool was decreased.
See 6.4.5 Wash pool Maintenance
for further process.
Cleanser A is expired or
with sediment inside.
Replace the expired cleanser A. If
sediment is seen please shake well
before use.
Wire between plug J4 on
main control module with
main board is not well
connected.
Re-connect the wire.
Main control module is not
powered on.
Use multimeter to check whether the
plug POWER on main control module
has +5V and +24v voltage input. If
there isn’t, see 4.2.2 Maintenance
and Replacement for further process.
The COM port number was
not well defined or was
changed.
The default COM port number of
pump-valve module is COM3.
Open a COM tools (sscom32E.exe)
to find current port communicated
with pump-valve module and if
necessary, change it to COM3.
Launch the system and go to
Management-System Settings-COM
setting, check whether the COM port
number of pump-valve module is
COM3.
Main control module was
damaged.
See 6.2.1 Main control board (02A) to
replace it.
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Symptom
Probable cause
Recommended action
The system
notice as ‘Valve
Test failed!’
while performing
valve testing.
The software conflicts with
the firmware of pump-valve
module.
Check the firmware version of main
control module. If it did conflicts with
software please contact the AVE
service engineer to ask for the latest
version and see 10.3 Upgrading the
module firmware to upgrade the
firmware.
9.3 Automatic sampler error message
No.
1
2
3
Symptom
The system
notice as ‘Lift
motor can't find
zero point’ or
‘Level motor
can't find zero
point’ while
launching the
system.
The system
notice as
‘Sampling
needle
collision’.
The system
notice as ‘No
Probable cause
Recommended action
The cable connected control
module of Automatic
sampler with adapter
module is loosening.
Re-connect the cable.
The photoelectric sensor
located at vertical zero
position error.
Clean the sensor. If necessary, see
7.5.4 Replacing up-down
photoelectric sensor to replace it.
The photoelectric sensor
located at horizontal zero
position error.
Clean the sensor. If necessary, see
7.5.4 Replacing up-down
photoelectric sensor to replace it.
Control module (02c) of
Automatic sampler error.
See 7.5.1 Replacing sampling control
board (02C) to replace it.
Main control module (02A)
was damaged.
See 6.2.1 Main control board (02A) to
replace it.
The photoelectric sensor
detected collision error.
Clean the surface of sensor, if
necessary, see 7.5.6 Replacing
sampling needle to replace it.
Fixed block of sampling
needle was loosening cause
anti-collision module
contact problems.
Tighten fixed block.
Collision is happened during
normal examination.
Check whether the test tubes are for
specialized use. Change them if they
are not.
If they are, see 7.3 Adjustment to
adjust the sampling needle.
The collision was happened
at wash pool.
See 7.3 Adjustment to adjust the
sampling needle.
The collision was happened
at other area.
Remove the obstacles.
The sample is not urine.
Only urine sample can be detected.
There is too little urine in the
The minimum volume of sample is
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Symptom
sample’.
Probable cause
Recommended action
test tube.
2ml.
The parameters of sampling
needle were not set
correctly or were changed.
See 7.3 Adjustment to adjust the
sampling needle.
Level detection error.
Re-connect the wire of sampling
needle.
Under power off status, Using a
cotton swab moistened with absolute
alcohol to swab the interface of
needle.
If the issue persists, see 7.5.6
Replacing sampling needle to
replace the sampling needle.
9.4 Microscope module error message
No.
1
Symptom
The focus is
unstable. The
cells in the
images are too
dark or too
bright, hard to
identify.
Probable cause
Recommended action
The LP or HP lens was
loosening.
Retighten the lens.
The installation location is
too close to the wall thus
heat dissipation of
instrument is not well.
The mechanism of
microscope does be affect
by the temperature.
Adjust the installation location
minimum 30cm away from the wall.
The normal examination only can be
start after 30 minutes warming-up.
The revolving nosepiece is
not properly located into the
slot.
See 8.3.2 Objective Adjustment for
adjustment.
The sensitivity of the
photoelectric sensor was
decreased or invalid.
Using a cotton swab moistened with
absolute alcohol to clean the sensor.
The belt of up-down motor
was loosening.
Adjust the belt adhesion to be within
operational limits.
The synchronizing wheel of
up-down motor was
loosening.
Tighten the 2 fixed screws.
The location of photoelectric
sensor or light barrier was
moved.
Adjust the location of photoelectric
sensor and light barrier to make sure
the light barrier is right in the middle
of groove. After this, see 8.3.5 Focus
Adjustment to re-adjust the focus.
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Symptom
The background
of images
became dark or
bright.
The system
notice as
‘Forward-backw
ard motor out of
range.’
The system
notice as
‘Left-right motor
out of range;’
Probable cause
Recommended action
The screw fixed at coarse
tuning nut was loosening.
Tight the screw to meet the standard:
Manually rotate the nut, the focus of
cells in the counting chamber is
stable.
The revolving nosepiece
was loosening.
Please contact the AVE service
engineer.
Maybe there is too many
dust stick on the surface of
lamp, collecting mirror,
stage or counting chamber.
Cleanup the dust.
The parameter of Focus
lamp was not correctly set
or was changed.
The default value of LP is 700 and
HP is 200.
The parameters of CCD
image were NOT correctly
set.
See 8.3.4 Image Adjustment for
adjustment.
The gears of collecting
mirror motor locked.
Lubricate the gears of motor.
The lamp was aging.
See 8.5.1 Replacement of
Micro-lamp to replace the lamp.
There is dust stick on the
surface of photoelectric
sensor, located at the zero
position of
forward-backward motor.
Using a cotton swab moistened with
absolute alcohol to clean the sensor.
The photoelectric sensor
located at the zero position
of forward-backward motor
error.
See 8.5.4 Replacement of Sensors to
replace the sensor.
The control module of
microscope error.
See 8.5.5 Replacement of
microscope main control board for
replacement.
There is dust stick on the
surface of photoelectric
sensor, located at the zero
position of left-right motor.
Using a cotton swab moistened with
absolute alcohol to clean the sensor.
The photoelectric sensor
located at the zero position
of left-right motor error.
See 8.5.4 Replacement of Sensors to
replace the sensor.
The control module of
microscope error.
See 8.5.5 Replacement of
microscope main control board for
replacement.
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Symptom
The system
notice as
‘Up-down motor
out of range;’
The system
notice as ‘Lens
motor out of
range;’
The system
notice as ‘Focus
motor out of
range.’
LP or HP images
captured are the
same.
Probable cause
Recommended action
There is dust stick on the
surface of photoelectric
sensor, located at the zero
position of up-down motor.
Using a cotton swab moistened with
absolute alcohol to clean the sensor.
The photoelectric sensor
located at the zero position
of up-down motor error.
See 8.5.4 Replacement of Sensors to
replace the sensor.
The control module of
microscope error.
See 8.5.5 Replacement of
microscope main control board for
replacement.
There is dust stick on the
surface of photoelectric
sensor, located at the zero
position of lens motor.
Using a cotton swab moistened with
absolute alcohol to clean the sensor.
The photoelectric sensor
located at the zero position
of lens motor error.
See 8.5.4 Replacement of Sensors to
replace the sensor.
The control module of
microscope error.
See 8.5.5 Replacement of
microscope main control board for
replacement.
There is dust stick on the
surface of photoelectric
sensor, located at the zero
position of collecting mirror
motor.
Using a cotton swab moistened with
absolute alcohol to clean the sensor.
The photoelectric sensor
located at the zero position
of collecting mirror motor.
See 8.5.4 Replacement of Sensors to
replace the sensor.
The control module of
microscope error.
See 8.5.5 Replacement of
microscope main control board for
replacement.
There is dust stick on the
drive shaft of left-right
motor.
Using a cotton swab moistened with
absolute alcohol to clean the drive
shaft. Lubricate the drive shaft.
The tabletting pressed at
counting camber bracket
were slipped.
Replace the tabletting.
The control module of
microscope error.
See 8.5.5 Replacement of
microscope main control board for
replacement.
Left-right motor error.
Please contact the AVE service
engineer.
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Symptom
The image area
appears blank,
blue, black or
blurred screen.
Or the system
notice as ‘Failed
to save
exposure!’ or
‘Open CCD
error!’
Positive or
weakly positive
samples were
examined as
negative result.
There are spots
on the captured
images.
The system
notice as
‘Microscope
communication
timeout’
Probable cause
Recommended action
The CCD communication
cable was not well
connected.
Re-connect the cable.
The CCD driver was not
well installed.
Re-install the CCD driver.
CCD was damaged.
See 8.5.3 Replacement of CCD to
replace the CCD and install the
driver.
The parameter of Dirty Spot
was not correctly set.
See 6.3.3 Blank Calibration to
perform blanking calibration. If the
dirty spots are higher than5 and they
are impossible to cleanup, see 8.5.2
Replacement of Counting Chamber
to replace the counting chamber.
The focus was not correctly
adjusted.
See 8.3 Microscope and Image
Adjustment to adjust the focus.
The fluid pipeline was
blocked.
See 8.4 Maintenance to check
whether sampling needle, pipelines
or counting chamber was blocked, if
necessary, perform maintenance on
blocked pipeline.
The mark of counting
chamber was not correctly
adjusted.
See 8.3.3 Square-Mark Adjustment
of Microscope to adjust the mark.
The background of image
was too dark or too light.
See 8.3.4 Image Adjustment to adjust
the background.
The spots are inside the
channels of counting
chamber.
Inhaling the disinfectant into the
channels and soaking for 3 minutes,
then perform Wash and Wash+. If the
spots are impossible cleanup, see
8.5.2 Replacement of Counting
Chamber to replace the counting
chamber.
The spots are inside the
CCD camera.
See 8.5.3 Replacement of CCD to
replace the CCD camera.
The communication cable
connected on the control
module of microscope was
not well connected.
See 10.1.4 Microscope Module
Diagram to check whether the cable
was well connected. Re-connect it if
necessary.
Power supply of main
See 10.1.4 Microscope Module
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Symptom
Probable cause
Recommended action
control module is not
properly.
Diagram to check whether MPW plug
was well connected. Use multimeter
to check whether the voltage
between two pins was normal. If the
voltage deviates from standard, see
4.2 Power System to replace the
main power module.
See 8.5.5 Replacement of
microscope main control board for
replacement.
The control module of
microscope error.
9.5 Software error message
No.
1
Symptom
Probable cause
Launch the
system and the
system notice as
‘Initialization
Error!’
An unknown error occurred
while communicating with
the database.
Recommended action
1
2
3
2
3
Login the
software, anyone
of below
messages is
noticed.
Failed to open
LIS port!
Failed to open
chemistry port!
Failed to open
microscope
port!
Failed to open
pump port!
Failed to open
sampler port!
Failed to open
reagent port!
Failed to open
barcode port!
The COM ports number
were not correctly set.
The system
661recg.exe is missing.
1
2
3
132
1
Restart the instrument to check
whether the problem is
eliminated.
Make a backup of all parameters
and try to uninstall and re-install
the database.
If the issues persists, contact AVE
service engineer.
Restart the instrument to check
whether the problem is
eliminated.
Check whether the COM ports
are existed. Go to Computer
Management-Device
Manager-Ports (COM & LPT),
and see 3.3.1 COM Setting to
check whether the COM ports of
every module are existed or
changed. Correctly set the COM
ports if necessary.
If the issues persists, contact AVE
service engineer.
Check whether 661recg.exe is
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No.
Symptom
Service Manual
Probable cause
notice as ‘No
recognition
program!’
4
The system
notice as ‘LIS
communication
failure’
Recommended action
2
The COM port number of
LIS is not correctly set or
was occupied.
1
2
5
6
7
Login the
software, anyone
of below
messages is
noticed.
Invalid Serial
Number!
Invalid
Password!
Authorization
validation failed!
An unknown error occurred
while communicating with
the database.
The system
notice as ‘Export
results to LIS
error!’
An unknown error occurred
while communicating with
the database.
The system
notice as ‘The
instrument is
out of control
during QC test,
adjust the
instrument and
retry!’
The QC measurement is
out of control.
The system
notice as
‘Abnormal Data:’
An unknown report error
occurred
installed under D:\AVE.
Contact AVE service engineer if
the file is not existed.
Check whether the COM port
number of LIS is correctly set or
was occupied. Re-set it if
necessary.
If the issues persists, contact
AVE service engineer.
Contact AVE service engineer.
1
2
Make a backup of all parameters
and try to uninstall and re-install
the database.
If the issues persists, contact AVE
service engineer.
See Chapter 6.2 Remedies on
Out-of-control in User’s Manual for
further process.
1
2
8
3
133
Copy the folder named Report in
update package to replace the
old one.
If the issues persist, re-install
CRRedist2005_x86.msi in the
rystalReports folder of software
installer.
If the issues persist, contact AVE
service engineer.
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Symptom
Probable cause
9
The system
notice as ‘Failed
to read or write
registry!’
An unknown system error
occurred
10
Login the system,
the system
notices as ‘Illegal
user’
The watch dog was not
installed or damaged.
11
The system
notice as ‘Open
DB error’.
Recommended action
1
2
The database was not
properly start.
1
2
Check the system registry file or
re-install operation system.
If the issues persist, contact AVE
service engineer.
Reconnect the watch;
If the issues persist, contact AVE
service engineer.
Re-start the database, if the issues
persist, re-install the database.
9.6 Other error message
No.
1
Symptom
After power on
the instrument,
the power
indicator of
monitor is
flashing but the
screen is
displaced
nothing.
Probable cause
Recommended action
The VGA cable of monitor
was not well connected.
Re-connect the cable or replace it if
necessary.
Memory has contact
problems or was damaged.
Re-connect the Memory or replace it
if necessary.
The monitor has problems.
Replace the monitor.
The Main board is
damaged.
Contact AVE service engineer.
The power cable of
instrument was not well
connected.
Re-connect the power cable.
The power button is not
switch on.
Switch on the power button.
Re-connect the wire.
2
After pressing
the start button,
the instrument is
not start up.
The wire of start button is
not well connected.
The start button is stuck.
The fuse is probably burned
out.
CAUTION: Beware of
electric shock! Plug the power
cable before operation!
Open the front panel and remove the
button-pad, then re-install it back.
Replace a new one with the same
specifications:
T3.15A 250V Φ5×20.
CAUTION: Beware of
electric shock! Plug the power
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Symptom
Probable cause
Recommended action
cable before operation!
3
4
5
Launch the
system, the
system time is
changed to
default.
The instrument is
shut down or
restart
automatically
during
examination.
The system
could not start
because the
system file is
missing or
corrupt
The PC power module error
See
4.2.2
Maintenance
and
Replacement to replace PC power
module.
The battery of Main board
was dead.
Replace the battery.
There is too much dust
stuck on the CPU fan.
Power off the instrument and cleanup
the dust.
CPU fan error.
Replace CUP fan.
CPU is overheating.
Add thermal grease.
The operation system error.
Make a backup of database and
restore the operation system.
The power supply is not
stable.
UPS device is recommended to use.
The system file is missing or
corrupt.
Restoring the operation system.
CAUTION: Contact AVE service engineer if you cannot resolve the issue
using this Troubleshooting guide.
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10 APPENDICES
10.1 Electrical Connection Diagram
10.1.1 Main Diagram
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10.1.2 Automatic Sampler Diagram
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10.1.3 Sampling Module Diagram
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10.1.4 Microscope Module Diagram
10.2 Upgrading the software
10.2.1 Upgrading the software
You will need the latest version of the software (a folder named “Update EN [Version]”)
provided by AVE.
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To upgrade the software, please follow those steps below.
1. Copy all the files in the Update EN [Version] folder and then paste to the Update files
folder which located in AVE SW Update.
2. Run the update program AVE Update.exe, then click Update button to start.
3. After that, the SW will be updated automatically. When the process is finished, click
Close button to quit.
4. After you upgrading the SW, it’s necessary to refresh the database in case to see the
modified wordings implemented in the SW. Please login the SW as ‘avekf’ user name,
with the password ‘ave’.
5. Go to Help-Init Config, and you will need to set up ‘Language’ as ‘0’, then click ‘Save’
button alongside.
6. Quit the SW and re-login the SW. If the User name appeared as Chinese character (as
shown below), which means the database was successfully updated. Then please go to
next step. Otherwise, please refer to guide for SW reinstallation to refresh the database by
re-installing the SW.
7. Re-login the SW as ‘avekf’, with password ‘ave’.
8. Go to Help-Init Config (Chinese version) again, and set back ‘Language’ as ‘1’. Click
‘Save’ button. Quit the SW and re-login it. Then you will finalize refreshing the database
and can continue to test.
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10.2.2 Reinstalling the software
There are some software errors that you can only fix by completely removing and
reinstalling the software.
CAUTION: It’s highly recommended that you make a backup copy of the
complete ‘AVE’ folder on a removable drive before completing the following steps.
To restall the software, please follow those steps below.
1. You may access AVE-764 installer file named ‘AVE Setup [latest released date]’
from local hard disk at F:\Tools\, or contact AVE service engineer to receive the
installer file via e-mail. Save the installer files to the F partition of local hard disk.
2. Login the software, go to Management-Backup and export the Urine Data and
Parameters to the directory.
3. Quit the software and stop running Database. Make a copy of the complete folder of
software to the directory.
4. Uninstall the software: On the desktop, click Start, click Control Panel, and then
double-click Add or Remove program. Find and click AVE v2.0 among the currently
installed programs, and then click Remove. If the operating system prompts you to
confirm the removal of the program, click Yes.
5. Find and double-click the setup.exe software installer file you saved in Step 1. Follow
the instruction that appear on the screen.
6. Login the software and go to Management-Backup, import the Urine Data and
Parameters to the directory you export to in Step2.
10.3 Upgrading the module firmware
10.3.1 Module firmware requirements
You may need to check if the version of module firmware is the latest one. To check the
version, it’s necessary to use a COM tools (sscom32E.exe) by sending the commands
below to check.
Module
COM port
Command
Pump-valve Module
COM3
FC 00 FC
Sampler Module
COM4
FB 01 FC
Module firmware requirements
Software version
Module firmware version
2.2.8.2015
Microscope Module:
XWH763B2.2H.hex
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Pump-valve Module:
Stand(2014-10-09)76(B)BF V1.05.hex
Sampler Module:
2014-10-30 764SY V3.41
10.3.2 Upgrading the module firmware
You will need the latest version of the module firmware provided by AVE service engineer.
To upgrade the module firmware, please follow those steps below.
1. Launch the system.
2. Plug the power cable off from the control module which you want to individually
upgrade.
3. You may need to use the tool STC_ISP_V486-english.exe which can be found at
F:\Tools\SOFTWARE\ STC-ISP-V486-ENG\ to upgrade the firmware.
4.
5.
6.
Set the MCU Type and COM ports for the module whose PCB you want to
individually upgrade according to the below information.
Module
COM ports
MCU Type
Microscope module
COM2
STC89C58RD+
Pump-valve module
COM3
STC12C5A60S2
Sampler
COM4
STC12C5A60S2/
STC12C5A32S2
Click Open-Code-File to browse firmware pre-copied in the device.
Click ISP Programming button to start upgrading. Meanwhile, the status area will
show the progress. Plug power cable on the control module when the status shows
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that powering on.
Reboot the device to complete the upgrading.
10.4 Installing or uninstalling the driver of CCD camera
You may need to install the driver of CCD camera after replacing a new one. The drive file
RZIma.inf can be found in the default installation path F:\Tools\CCD\Driver for 300
20130325\.
Just follow the Windows process of driver installation or uninstall to perform your
operation.
10.5 Reattaching and reinstalling the Database
There are some software errors that you can only fix by reattaching the Database.
Complete the steps below to reattach the database.
CAUTION: It’s highly recommended that you make a backup copy of the
complete ‘AVE’ folder on a removable drive before completing the following steps.
10.5.1 Reattaching the Database
Removing the error Database
1. Login the software, go to Management-Backup, and export the Urine Data and
Parameters to the directory.
2. Quit the software and go to Start-Microsoft SQL Server-Enterprise Manager. Open
SQL Server Group-(Local) (Windows NT)-Databases in turn.
3.
Right-click on the error database and click Delete to start removing the database.
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4. After the removing, click OK and reboot the device.
Reattaching the Database
5. Right-click on Databases, in the drop-up menu, click ‘Attach Database…’ option in
the sub-menu of All Tasks.
6.
In the jump-up interface, click ‘…’ button for attaching database.
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7.
Browse AVE2_Data.MDF which is saved for attaching. click OK button to continue
the next step.
8.
Click OK button to start attaching the database.
9.
If the database attachment is complete successfully, click OK button for confirmation.
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10. Double-check that the new database named AVE2 already exists under Databases.
11. Login the software again and go to Management-Backup, and import the Urine
Data and Parameters from the directory.
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10.5.2 Reinstalling the Microsoft SQL Server
CAUTION: There are some software errors that you can only fix by
reinstalling the Microsoft SQL Server. In this case, please contact AVE service
engineer for further assistance. Do not uninstall any of system program without
prior permission from the AVE service engineer.
CAUTION: It’s highly recommended that you make a backup copy of the
complete ‘AVE’ folder on a removable drive before your operation.
10.6 Backup and restoring the Operation System
It makes sense to make a system backup regularly in case that the operation system is
crashed or there are some serious system errors that you can only fix by restoring the
operation system or individual partitions of hard disk.
10.6.1 Backup the operation system
1.
Reboot the device and select ‘1KEY GHOST v2014.07.18’ in the startup menu,
select ‘GHOST, DISKGEN, HDDREG, MHDD, DOS’ in the screen, select ‘GHOST
11.5’ in the next screen, select ‘IDE/SATA’ to switch to the operation interface.
2.
To backup the complete individual partition of local hard disk, go to local-Partition-To
image, a selection window will pop up. Select the local source drive by clicking on the
drive number. Press CLICK to select OK button to continue.
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3.
In the pop-up selection window, select source partition from basic drive, and press
CLICK to select OK button to continue.
4.
A window for creating the copy name and selecting the partition that copy to will pop
up. Press CLICK to select the destination partition and to input the copy name. Press
CLICK to select Save button to continue.
CAUTION: It’s highly recommended that you copy the image file copy on F
partition. Make sure that there is enough capacity remained.
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5.
If the operating system prompts you to confirm the compress image, click ‘Fast’ and
then click Yes.
6.
If the operating system prompts you that the process is finished successfully, click
‘Reset computer’.
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10.6.2 Restoring the Operation System
CAUTION: It’s highly recommended that you make a backup copy of the files
that you want to keep on a removable drive before your operation. Make sure that
you have a image file that created before in the local drive or removable drive.
1. Repeat Step 1 in 10.6.1 Backup the operation system.
2. Go to local-Partition-From Image, a selection window will pop up. Select image file
that to be restored from source partition. Press CLICK to select Open button to
continue.
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3.
In the pop-up selection window, select source partition from image file, and press
CLICK to select OK button to continue.
4.
In the pop-up selection window, select local destination drive, and press CLICK to
select OK button to continue.
In the pop-up selection window, select destination partition that you want to overwrite
from basic drive, and press CLICK to select OK button to continue.
5.
CAUTION: To restore the operation system image file, it’s highly
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recommended that you select C partition as the destination partition.
7.
If the operating system prompts you to confirm your operation, after re-check that
your operation is right, click ‘Yes’.
8.
If the operating system prompts you that the process is finished successfully, click
‘Reset computer’.
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10.7 Maintenance kit
No.
Kit
Quantity
No.
Kit
Quantity
1
slotted screwdriver
1
13
multimeter
1
2
slotted screwdriver
1
14
portable hard drive
1
3
Phillips screwdriver
1
15
Brush
1
4
Phillips screwdriver
1
16
Cotton swabs
5
Phillips screwdriver
1
17
Nylon ties
6
needle-nose pliers
1
18
Electric iron
1
7
Diagonal cutting plier
1
19
syringe
1
8
tweezers
1
20
rag
9
Chip Clip
1
21
latex gloves
10
Open spanner
1
22
Lubricating oil
11
Hex Wrench
1
23
adhesive
12
test pencil
1
24
Lens wipes
10.8 Recommended spare parts
Code
Name
101990021
101990018
110012007
205010876
201010809
201010810
202010804
202010805
202010806
LENS 10×
LENS 40×
BRACKET PLATE
CONTROL BOARD (MICROSCOPE)
PHOTOELECTRIC SENSOR
PHOTOELECTRIC SENSOR 4P
COLLECTING MIRROR MOTOR OJ5
LENS MOTOR OJ4
ASCENT & DESCENT MOTOR OJ1
FORWARD & BACKWARD MOTOR
OJ2
LEFT & RIGHT MOTOR OJ3
BELT 80MXL
BELT 155MXL
PULLEY 1A763.4.1-06
PULLEY 2A763.4.1-08
PULLEY 5763.4.1-03
SPRING TABLET
LAMP
MICROSCOPE
CCD CAMERA
CONTROL BOARD 02A
CONTROL BOARD 02B
203030801
203030802
110084009
110084002
110085008
110085010
110085013
110011019
101110009
105100002
105100005
205040003
205040001
153
Maintenance
/Service
S
S
S
S
M/necessary
M/necessary
S
S
S
Amount/10
analyzers
1
4
2
S
S
S
S
S
S
S
S
M/2000hours
S
S
S
S
(1)
4
(1)
1
1
AVE-764
205040004
205040002
312011002
204020801
204020801
203020802
203020801
204010801
204010802
204010803
204010806
204010805
204010804
204010807
204010808
204010809
204010810
202010802
111000002
111000005
199000040
111000006
111000004
110082001
110855036
110855033
110086002
110082005
110082007
110855037
110855038
110855039
110855034
103010005
103010004
201010807
Service Manual
CONTROL BOARD 02C
ADAPTOR BOARD 02E
WASH POOL COMPONENT
MAGNETIC
VALVE(BIDIRECTIONAL) DZ1
MAGNETIC
VALVE(BIDIRECTIONAL) DZ10
CLEANSER PUMP MJ1
WASTE PUMP MJ2
LEFT VALVE (CH1) DZ32
LEFT VALVE (CH2) DZ42
LEFT VALVE (CH3) DZ52
RIGHT VALVE(CH1) DZ31
RIGHT VALVE(CH2) DZ41
RIGHT VALVE(CH3) DZ51
CLEANSER A VALVE DZ9
CLEANSER B VALVE DZ7
MAINTENANCE CLEANSER VALVE
DZ8
AIR VALVE DZ2
CLEANSER DETECTIVE
COMPONENT
PUMP TUBE
CHAMBER PIPELINE
WASTE PIPELINE
PINCH VALVE PIPELINE Φ1.6*3.2
PINCH VALVE PIPELINE Φ2*4
REDUCING CONNECTOR
BULKHEAD CONNECTOR NUT
RUHR CONNECTOR PMLF31
RUHR CONNECTOR LM41
T-CONNECTOR
Y-CONNECTOR
GREEN NYLON COLOR BANDS
BLUE NYLON COLOR BANDS
BLACE NYLON COLOR BANDS
POLYPROPYLENE COLOR BANDS
FORWARD&BACKWARD MOTOR
ASCENT&DESCENT MOTOR
ASCENT&DESCENT ZERO
POSITION PHOTOELECTRIC
SENSOR
154
S
S
S
1
(1)
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
M/6 months
M/necessary
M/necessary
M/necessary
M/necessary
M/necessary
M/necessary
M/necessary
M/necessary
M/necessary
M/necessary
M/necessary
M/necessary
M/necessary
M/necessary
S
S
4
4
4
4
4
10
10
10
10
10
10
10
10
10
10
M/necessary
4
AVE-764
201010808
205040005
102100054
201020821
110084033
110084032
205030001
205030002
205030003
205030004
114990017
110081015
110081012
110084031
110084028
203010714
203010715
101090025
101090011
205030005
105010002
105060002
105020002
105030005
105041003
105081005
105110003
105110004
105050004
110063001
110058009/
10/11
Service Manual
FORWARD&BACKWARD ZERO
POSITION PHOTOELECTRIC
SENSOR
ADAPTOR BOARD 02D
SAMPLING ARM CABLE
SAMPLING NEEDLE
BELT
189MXL025-236Z189MXL025-236Z
BELT
165MXL025-206Z165MXL025-206Z
CONTROL BOARD 03A (SAMPLER)
PHOTOELECTRIC SENSOR CA
(JR2,JR3,JR5,JR6)
PHOTOELECTRIC SENSOR CB
(JR1,JR4)
PHOTOELECTRIC SENSOR CC
(JR8)
COLLISION BEAD
LINEAR SLIDES COMPONENT
(MR9MNSS1V0N-190L-5-5-1)
LINEAR SLIDES COMPONENT
(MR9MNSS1V0N-80L-10-10-1)
BELT 146MXL025146MXL025
BELT 64MXL02564MXL025
MOTOR 35
MOTOR 42
POWER SWITCH
PHOTOELECTRIC SENSOR
(JS1,JS2,JS3,JS4)
PHOTOELECTRIC SENSORI (JF1,
JF2)
MAIN BOARD
M/necessary
4
S
S
M/necessary
1
2
2
S
S
S
1
M/necessary
4
M/necessary
4
M/necessary
4
S
S
S
S
S
S
S
S
M/necessary
4
M/necessary
4
S
1*
PC POWER MODULE
CPU INTEL G2020
MEMORY 2GB
HARD DISK
CPU FAN
KEYBOARD
MOUSE
MONITOR
CHASSIS
S
S
S
S
S
S
S
S
S
PANEL
S
155
AVE-764
102061032
110011029
113080009
101100003
110063012
205010846
109060004
205010802
101990041
105081010
101090005
205010803
106010032
Service Manual
WASTE DETECTION CABLE
COUNTING CHAMBER (3
CHANNELS)
TEST TUBE 16MM*10CM
FUSE T3.15A Φ5×20
TEST TUBE RACK
WATCH DOG
BICONVEX MIRROR
LEVEL DETECTION MODULE
BARCODE READER
FAN 120*120
MAIN POWER SWITCH
PC POWER PINBOARD
POWER MODULE
x=Important on stock
(x)=recommended on stock
x*=if option installed
S
M/necessary
Consumable
S
S
S
S
S
S
S
S
S
S
(2)
2
(1)
10.9 Operating AVE-764 and urine analyzer together
Before start operating the two analyzers as a unit, their data cables need to
be linked and/or their rack movers need to be connected. Consult AVE service
engineer for further assistance.
Make sure that the system time of urine analyzer is same with that of
AVE-764.
To operate AVE-764 and urine analyzer together(Urilyzer Auto for example)
1.
2.
Install the Uritrail.
Connect Urilyzer Auto to AVE-764 with a serial COM cable as shown in below figure.
156
AVE-764
Service Manual
3.
Login the software and go to Management-System Settings-COM Setting, click
Urine Analyzer and at the right area configure Bit rate, Data, Parity and Stop that
the same with Urilyzer Auto. Click Save button then quit.
4.
Go to Management-Chemistry Setting-Chemistry Item, configure chemistry items
in Parameter and Abbreviation columns that same with Urilyzer Auto.
5.
Go to Management-Chemistry Setting-Chemistry Protocol, choose Urilyzer Auto
at Apply area, then click Save button at Apply area. Meanwhile, keep the four
options (Start Symbol, Transfer ID, End Symbol, Transfer Date) unchosen.
157
AVE-764
6.
7.
Service Manual
Click Test button, then transfer a result from Urilyzer Auto to verify if the connection is
proper. If the data can display at the testing area, go to the next step. If there is no
data displayed, please check the COM cable connection and the communication
settings.
Login microscopy interface and start examination, transfer a result from Urilyzer Auto.
Check whether the result can be displayed. Contact AVE service engineer if the
communication is not proper.
158
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