MEMBRANE AND SOLUBLE LOW AFFINITY IgE RECEPTOR CD23

Inmunologia1997t16r87-94
MEMBRANE AND SOLUBLELOW AFFINITY IgE RECEPTORCD23
EX?RESSION,CYTOKINE PROFILESAND SERUMIgE lN BOTH
AI,LERGIC REINITIS AND ATOPICDf,RMAIITIS.
Thssinan Paolo,MD, Larin Mrria, MD, Blanca lsaac, PhD! Birnco Nicohs, MD
and Aldrcy Oscar.MD.
rh. nenblhe rowsmiity rgr ftp'oi (cD23),
ns $rubrerracfmr GcDrr d rsEbiidinsrdd),
r err4kin4 (r14) andrncrftmny trrN-, {e aNide
dy$eguldionor.helg!sydhesisa
tcd rr. n.mbra!c
aDdlN.lFofrylhredopicFli46
areqic rbhniseAR) .Dd r6 wnb .bDE demadfs(^D)
werc sered.d. r{ei,y r'esr,hy ioi 3bpi. $bj.ds r.r
oino\y.,ton.lr,,
wh'le scD23, IL 4' IgE
d IFN,I
Er !d*P'q ddgE & b3j3ariDidad
cDn. $
rrrenedo solobhGcD23o fadoi de uni6nde reE),
ineneucinia (I-a) e inbdeljn / (lFN /), sonoNidem
.oitrmsltdcidneihsiitsisdcle!y.dic|tipodc
cosde scD:r,lL-4,lgE e lrx Y. seleccion.mos
4l
ias peEme(PAR)
y 16..Ddcmritn lejpiQ(AD) Es
a signilicant increas tn
hd pdidh)
otrpThe.,tokines mern de nujoririardo r. lldica demdmje dobrean
'r childrcn (contuls h'icurrd Edodm,16 $Fcinos enjusdosan dos
dhibn.d lishd nlus ofscD2r rhaD
s€.$ descD2r, IL4, lei e IIN l tuercndednmdos
E synrh*b in boi\
tud.ibdd! dc linrd.nos
B cDr9+.n orFpo dcniis,
(eytord!:rowstfiniiyIgEreftPbB'solublecD:3'
de|osiive|sslicddelcE,oilosn|oefisslbsd.
IsEenAD L.s nivd* dehs cnoon
ninosGontulesy prcEftt
Phoiedd fixi (s32)6rr.0rr
exhibierc
Inmunologi. 1997j16:87-94
rrlororreE i! rb. ncnbEneroy aijniry rec@ror
for
rios in cD} prcddioi fry diy r tul! ii dr pdhoed.
cle edto producesluble prcducbGCDI) win\ mole
|etsidnonoc}res.cD2]nedistslg
lar crtoroxiciqraeRifr plunes r s wen $ IgE depei'
$if trlsrioiby cnviroincntdlantise( (alhrses) i.
PeFmialrlleek dinhh (PAR)h.o
innediithypelsnsnivityfacliotr:
odi'n (aDl is a chroDicsEnabus reeron chnmr
on hgenpres rg €lls (L gdtuns 3ndB
pr$mErion
rtnsl. cD2r 6cu$s o! 'he rgE-deDentlenr
od md an asociarionwin\ orherfoms of dopy i. ihe
dinsoflgE by membrane
boondc
aiimlivilyappe$
tiriesinvohesde
obulii E (reE)pro.
sion ofmenbEDe
rcg''ldiono|IgEsydhdhii.|udingstsl.dllyp.s
md \mphokiB. o,re fadd oNidercd tr a naFi rclu
1 - b 1 ? P h ? r c ,p t c a M ! , ; , o t P B u t ; 4 . h t L ? n t p a ' e ' r v , - a m o : l
AD
6380+s46
2769t86
CD3+
cD/l+
cD8+
cDl9+
cD56+
3817+469
25111299
2142+162
1096+t96
77|tt62
246+31
27t2
I0+2
ll,
66+3
1 3 ? 2 + l 8 l 36+2
915+166
16'
652+149
4227+501 43+5
3009+163 1lJ2
36+:l
10561202 24+3
116!83
13+2
r
5q+79
i4+l
Inmunologia1997i16: 87-94
tabb
cDl+
cDl+
cD8+
cD19+
cD56+
Ph"aaDpi.dha\t6aIPBv. i a.]Ltut/la, .r^ \ ' .aaro '
5980+,113
2033r150 34!2
r469+102 73+2
8?l+76
21:2
to+l
6461+233'
qL 21611110
2292.+258 3 4 + 3
itu2
l6t3+156
?3*2 ti79*38! 58+2' I
4l+l
738121 3t+t{
h
5691?8
25+2 645+IA3' 21+2
b
265+50
410175
lt+l b
r8+2
184+56
62t+t74
8+2
26!?
e{cN$@np{dtaobt'pjG{holqrd!9ih|d!o}P{slkl.dFd
smm IgE rryek veF analFedby Quntidone
rcEtuA ki' (hrre{sd Di4ldiiq chlsri MN. usA)
Arkrgic Diseassout dGnr crhlc orihe hniiure or
r\€nty sve, weF d,ildren(< r: year): la hadPAR(6
mxls lid 3 rcmls. fdi rsd 7 + ' ycf) hd ts h,,l
AD (4 nxrs md e ftn,rn, nsi,ge 6 + 1 yert.
sixEenpdErh s€re adulu i 3 hrd PAR(5 m?lesi,'d 3
.cm!rB,nsD agc22+ 1ys6) trd I h.d aD 0 Fares
I yssl An p,'i.msh3d
ssitrophiria(>3s0ssinophirs/mf). raAirk inftdioi
gro@d der cobr no! cybrdry (EPrcs-75r
coor's
colpon'ioi, Hide.h FI UsA)
cD56od cDn (cod€r coDoruhot rtE addedto
psiphenr brmd nmo[d*i
Grs cBrlrc) obbinedby
ko!]@mddifl.rmtia|mmhv{eI
er5% or obrc[s) vn! mivd*l
atd lainae. laba.co,
s nix) ndror rood
poh
(cell rft .D2r d kr'. r cell Di€no{iq
rnigenslchocoh'e,corn,nce,
itrmossy
sh lnd ci'rus mn) hrccambndgeusA)i mnnil {lus {ere bedrd ls io
(r.rolisd s'iei hboi&tries, hokme, usA) *ne eri.i
250 U r hn. serumrL-4 sas ne$ured by ELrsA
(Huhh lL'1 ELISA rc! ki'. ritd(cl
6cizyn.
(4 co'lonhm, cambndge,usA)r ur"
rhc conrolsreupcosisrd of i0 chirdren
nsrs ad 6 i.far*, nsi lge r + ' yes) md 103dtrr6
(5 mar$ and 5 remar$,meanage,e + 2 ysEl none
$id*kr' ELnA ndhod (Idens.y Huni IFN 1 kit,
lDd,Etoph4oid{
rkrcn$in's
hmunologia 1997i16: 87-94
Tabk 3. Phenoqpic analrsisafPBMq CD23in .hildten (paieh^ f .annots)
PBMC/CD2]T
cDt+/cD23+
CD4+/CD23+
i
129+3s
3+3
r.l+0.3 2r2+54
0.2ta.l
cD8+/CD23+
cDl9+/cD2l+
cD56+/CD23+
0
t
2
!:2
a2
0.3+0.1
0.2+0l
0.140.1
2.8+O
5|
0ia0.3
cenzyme co4orrhon. carnbridee,usA)j vatus rie
(pr0 05) vh.i r,mD.Ed ro .ofuors (.arl. 4 rn idrlrs
evxtr'aredby 4 unprned ioiprBmdic
ren (Mrii
wihEy csl v:ruc! or 'obr rsE!e€ rogbBromxd
di,ni,non or $c pqenbgeorcDr+.drh (p<0.05) d
ctu+ eLls (p<00i) lrltb 2)
*nDc}rok'iesw$gvarudedbyrl'ePg*ont$'
tp<o 00r) hd Ftrcutarly orcD
Tbd|benotyprcrialysiso.PBMcincoftols
h<0.00r)in borr,PARlid AD child
I exhibiredsimitiL 'o .onto\ llarle r). PAR !tu1 AD aduusshowedh
cll$bpopulaiio$ hlles (Iodr. 1, .2,,, J) Ii rlxrjon
cDle+/cD23+ .elrs ,b!n dritdretr (p<0.005) (/4nr. ,r).
sMr
lev.k or 'cE. scDI,
rr.l
d rFN ( rcE m$s
\I])etrevRlndined]eFEMCpheiotyplPrcfi|{!
icaas of rohl cD rs+ B ens 0<0 0t \rb6 cooDed
ro .oitroh trafi' 4
rors (r4d,,r), beiig'hisdjrieEn* sicdfisil oiy ibr
PBMc/cD23+rtur (F0.05) qhetr.odprrodb rAR
(/a6l.,) rimLly.rhs co
chird.do
adurr. h vrs sisiifiud tnu{sd ii childrdi tndepen
dedr o. d,e rgE revds (rorl. 6l
hisb4 $?n r.000u.' /mr(r4re 7)
Inmunologia1997;16187-94
ok for iond $bjeds I n. w. !trd
riduils(10childEtr 'd 10aduht*i
bqdhryicdfedeorDmsilc'nrefonil'droudsigni
ficsiily hisherwlu* orcDre+/cD2r+ellr {r% 6
0%) in conror .dul.s wl,q, co,npmdro ch dH rhus.
abrishrheiandard
A[ pdidds crbibneda sign]ficmiincFasem
rhe ml'H of leE (!<0.001 Rhei o
G n irun
iowcr slu*
Llds o. 5cD23, tL.4 and rFN y besen
o.scD2r 'n$ chirdFn (rad. r).
scD2rsd rndi.nokri6.
whil. PARldurr!showed
rhc rowdnDiq Eeprorfor rgE,F€Rr,'cD2r,
ua desnbedrbr irrefinr tnneby s
rhebsi. dtu.rurs in rhcEsurdioi of rgEsynnEsn.
Tl'ecD:]mokcu|eisrlyp€]Iideellnedb,t.gly'
|Frphocy€s'cD:3Bse|ed'velyex
rgM/rgDbs;Ds cdrs.Dd i ro$ rner so\Te svrchin!
*luc of cDzr i \mphcrts
or nonml $bjeds nn
aseorbolh PBMcrcD2r: aDdcDi9/cD23+cdh in
lFphoc]tsisofchildHTl'geaF$vdglrcponssho
Table1 Phehorwicanalrsis '[PBMC/CD23+ ih aduks (Patienls$ connots)
PBMS/CD23+
cDl+/cD23+
cD.l-./cD23+
cD3+/CD23+
cDr9+/cD23+
CD56+/CD23+
t.7t).2
l+l
0
2+2
0
0
tjjn 2
58+11
2J+r.8
0
5:l+i4
2.3+0.3
124+4)
0,t101
0
0.1+0l
0.r+0.1
1.5+140
03111.1
91+21
0.t+0.1
0
01) srE5 dejL
\d
Nid!
fl{qene
by
lnmunologia1997i16: 87-94
d ler4r ii fte qle ol811 rhd .xpF$ rhe
epror B
suggsr ft dopic plrieit sl'ouldrl
.olldheuaisanmk4ofdopy,n
N4 inv4rj$io.s shouldb. mde ro ddemiL
ol
.hln, ruhr rh. difierer@sin the inmunopdhdroey
hrting racrorFofrhmot. saD2r m
tioNof|L-lwilhrccyc]ed[.lKeptou!h.oitn$,
JFN-Idd IFN't iDdurrtsi' ,ibn ll.a indmedcD2l
pa6stbysflmtgltc|.{efomd
didr (rark 6);r
\ilh lgE leversbiehr rhrn 1000! rrrl(Iar,.
7)
cDlr) in di$lse ssociatd \ith eLev*ed
Goluble
IeE,clin'EpA1|j91939!:0:]95.4'
bindidefrdos'rmmdd|'Todl}'1939.'0:'9165
]'Ddb.lonhA,Beieded.SmithR.crprcl^,d!|
rhcdprcsior or
Pftv.dionbyLrlockingritibodEsof
id'nudtyinlDiechildrcn.P lsi|o|og}'193?i94:
Table5 thhrhaseralogi.ol erdlwti.h oJpatientsahtl conialt
CHILDRIEN
fu$tedldsnw:fuddmljp{c
ADLILTS
InmunoloqiaI997; l6: 8?-s4
1 t , t ? tt p ^ ! a
o
DJo'
0-4"11
"b?ti'I4a"olto:C.lc.lr.hd'e1
PATIENTS/CHILDREN
IgE=o-130
ceLlPopulalioN
nnr
%
cMsP/cD23+
cDt/cD2l+
21i1
0
A 264+96
0
lllll
o t+o.j
CD4+/CD23+
0
0
CD8+/CD23+
cDl9+/cD2l+
cD56+/CDll+
'rbb
l+l
0
0
IsD- 180-1000UL/ml
9+l I
6+5
0
0
0
129+92
0
0
0
2+l
lmr
%
mr
CMSP/(D.21 154 )0
1t.D
cDl/cD23+
l + l 0 0
16+6
0 5+0.2
10:6
0.1+0.2
0.2+0.2
r b 2 , , , r - ' . o ao t a L ? , e - a d L t ^
IgE>l000Ul/nl
Yo
)A:
0
C D 4 + / C D 2 3 + 0 0 0 0
d
4
2+2
C D 5 6 + / C D 2 3 + 2 1 2 0 0 0 0
(dirinl
t24+3t
0.110.01 l5+12
3544
l+l
0
C D 8 + / C D 2 3 + 0 0 0 0 t2
cDl9-./cD2l+ 25+6 l+O 35+16 2+05 5q1t?r
@d! mr ddrdu!
0.5+0.2
6*5
PAIIINIS/ADULTS
gE=180-10
0L
l e E = o8- 0
U0l m
Cell Populaliom
1616
t6o152r 4+1r
,LaJti.pa..
1,p.,,"q.f.D
IgF>l000Ulnl
or Fhse
0.110.01
7+6
0,3:0.1
9441
0
Inmunologi.l99T; l6: 67-34
frbr
EpslonFJI banns ells genrae JsE.binding
1s39i
of diiTdet nolold reients Mol.lmmd
1?.Lderliq M, Nakajimar. Purilro.ccjuddc. Horft'u
9'PimiU,s.hltr.hT.Pn[J,Ri.b
affiee. focusineby humr B lrrphocts
n media-
REombjnadre1u61c
F! cFildn rcp& n (F. .psiloi RIrcD23) hA IgE bi"diry activ'tybut no B .ell
adiviq. J.Immunol.1939:Ia2:
srcvrhprcmoting
IgBbindi.e rado6 GolublecD23) IIL Eudn* r.r !
adivi'y r.
Fceptor(FcERn)hso.in d prorloLy'i.
*ptsed
oi nsn'rc B .ells berorcisoqpeswiEhi.s
I9 LcungD. Rhddds
A. GehlR EimdiionorT
cll
nng lrbpho.}ls ii dle€k .id mn alleerc dnldrcn.
or soluble
cD23G cD23).r
plaesin rheDomalpoDulaljoN
Clin Eip rhnuiol
(cD23)lrbibit 'he sponr!reoBmig*rjonor!''?
nonocylic.e|kjNeutdizdionofMlFelivitytly3
Solublelow .tiniq
cDr0+cD23+ rymehocyrsii eioprcsubjecr {, l
elevdedsenm lgE concentdions.Ann arGrgy
t H, DFson o,
iidribr. ritoyeck. r Bior cl'dn. 1e3er264:6e34
(cD2r) oi iomxl hman B lynphocyEsr, Exp.
ronnii'on of lsE Ln'd'ngf.c6's GolublecDzr). L Fc