Fluorophore Selection Guide

Fluorophore Selection Guide
for Laser-Based Instruments
UV
IR
300
400
500
700
600
7-amino-4-methylcoumarin (AMC, AMCA)
Alexa Fluor® 405
Alexa Fluor® 488
Alexa Fluor® 532
Alexa Fluor® 633
Alexa Fluor® 350
Alexa Fluor® 405
Cascade Blue®
Cascade Yellow™
Alexa Fluor® 514
Alexa Fluor® 532
Alexa Fluor® 546
Alexa Fluor® 555
Alexa Fluor® 635
Alexa Fluor® 647
Cascade Blue®
Dapoxyl®
Alexa Fluor® 610–R-phycoerythrin (R-PE)
Alexa Fluor® 568
Alexa Fluor® 660
Cascade Yellow™
dansyl
Dapoxyl®
dialkylaminocoumarin
hydroxycoumarin
Marina Blue®
Alexa Fluor® 647–R-phycoerythrin (R-PE)
Alexa Fluor® 680–R-phycoerythrin (R-PE)
BODIPY® 493/503
Alexa Fluor® 594
Alexa Fluor® 610–R-phycoerythrin (R-PE)
Alexa Fluor® 647–R-phycoerythrin (R-PE)
Alexa Fluor® 680
Alexa Fluor® 680-allophycocyanin (APC)
Alexa Fluor® 700
dialkylaminocoumarin
Pacific Blue™
BODIPY® 505/515
Alexa Fluor® 680–R-phycoerythrin (R-PE)
Alexa Fluor® 700-allophycocyanin (APC)
Marina Blue®
pyrene
PyMPO
BODIPY® 530/550
BODIPY® FL
BODIPY® R6G
BODIPY®
BODIPY® R6G
BODIPY® TMR
Alexa Fluor® 750
Alexa Fluor® 750-allophycocyanin (APC)
allophycocyanin (APC)
Cy2
BODIPY® TR-X
BODIPY® 630/650
Cy3
BODIPY® 650/665
Cy3.5
eosin
Lissamine™ rhodamine B
Cy5
Cy5.5
naphthofluorescein
Selection Guide Key
eosin
fluorescein (FITC, CFSE)
NBD
Oregon Green® 488
Excitation Range
Rhodamine Green™
Excitation Maximum
800
Rhodamine 6G
Rhodamine 6G
R-phycoerythrin (R-PE)
Rhodamine Red™
R-phycoerythrin (R-PE)
tetramethylrhodamine (TRITC)
Texas Red®
X-rhodamine
Emission Range
UV
Emission Maximum
405
488
532
633
UV
300
IR
400
500
600
700
800
7-amino-4-methylcoumarin (AMC, AMCA)
Alexa Fluor® 350
Alexa Fluor® 405
Alexa Fluor® 488
Alexa Fluor® 514
Alexa Fluor® 532
Alexa Fluor® 546
Alexa Fluor® 555
Alexa Fluor® 568
Alexa Fluor® 594
Alexa Fluor® 610–R-phycoerythrin (R-PE)
Alexa Fluor® 633
Alexa Fluor® 635
Alexa Fluor® 647
Alexa Fluor® 647–R-phycoerythrin (R-PE)
Alexa Fluor® 660
Alexa Fluor® 680
Alexa Fluor® 680–R-phycoerythrin (R-PE)
Alexa Fluor® 680-allophycocyanin (APC)
Alexa Fluor® 700
Alexa Fluor® 700-allophycocyanin (APC)
Alexa Fluor® 750
Alexa Fluor® 750-allophycocyanin (APC)
allophycocyanin (APC)
BODIPY® 493/503
BODIPY® 505/515
BODIPY® 530/550
BODIPY® R6G
BODIPY® TMR
BODIPY® TR-X
BODIPY® 630/650
BODIPY® 650/665
BODIPY® FL
Cascade Blue®
Cascade Yellow™
Cy2
Cy3
Cy3.5
Cy5
Cy5.5
dansyl
Dapoxyl®
dialkylaminocoumarin
eosin
fluorescein (FITC, CFSE)
hydroxycoumarin
Marina Blue®
methoxycoumarin
naphthofluorescein
NBD
Oregon Green® 488
Pacific Blue™
pyrene
PyMPO
Lissamine™ rhodamine B
Rhodamine Green™
Rhodamine 6G
Rhodamine Red™
R-phycoerythrin (R-PE)
tetramethylrhodamine (TRITC)
Texas Red®
X-rhodamine
UV
300
63150
IR
400
500
600
700
800