pDRIVE5-GFP-10

XbaI (15)
EcoRI (19)
NsiI (14)
SdaI (29)
NotI (2)
BssHII (137)
SalI (304)
SpeI (308)
BspEI (414)
HindIII (573)
hAldA enh
pMB1 Ori
hFerL prom
BspLU11I (3446)
BssHII (3275)
EM2K
BglII (933)
SmaI (3225)
Zeo
pDRIVE5-GFP-10
(4194 bp)
BspLU11I (1000)
chEF1 5'UTR
SacI (1195)
SmaI (1217)
XhoI (1332)
SV40 pAn
GFP
AgeI (1559)
NcoI (1567)
Acc65I (1789)
NheI (2290)
110
NotI (2)
XbaI (15) SdaI (29)
NsiI (14) EcoRI (19)
1 GCGGCCGCTATGCATCTAGAATTCCTGCAGGCGAAACTCAGCTTCCTTCGTTTCCGACTTTTCCATCCGCGTCCTCCACTTCCCCGTTCCGCCCTCCCCC
BssHII (137)
101 ATTGCCAACATTCTGGCTGAGTCACGGCGCCCCAGAGCGCGCCAGGCTGGGGGAAAGGAGCAGAAGGGAGGGCCCTAGCGACCCGCGGGATGTGGTCCGA
201 GTCACGTCCGAGGGGGGTGGGGAGGGATCGTGTTCTCGGCGCCCGCCCCTTCCTAGCGCGGCCTCTGGGCTGCGCCTCTCGGGGGCGGCCCGTAGCCCAG
SpeI (308)
SalI (304)
301 TCCGTCGACTAGTCAGGGCCCCAACCCCCCCAAGCCCCCATTTCACAACACGCTGGCGCTACAGGCGCGTGACTTCCCCTTGCTTTGGGGCGGGGGGCTG
BspEI (414)
401 AGACTCCTATGTGCTCCGGATTGGTCAGGCACGGCCTTCGGCCCCGCCTCCTGCCACCGCAGATTGGCCGCTAGGCCTCCCCGAGCGCCCTGCCTCCGAG
HindIII (573)
501 GGCCGGCGCACCATAAAAGAAGCCGCCCTAGCCACGTCCCCTCGCAGTTCGGCGGTCCCGCGGGTCTGTCTCAAGCT TGCCGCCAGAACACAGgtaagtg
601 ccgtgtgtggttcccgcgggcctggcctctttacgggttatggcccttgcgtgccttgaattacttccatgcccctggctgcagtacgtgattcttgatc
701 ccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgcttaaggagccccttcgcctcgtgcttgagttgaggcctggcttgggcgctgggg
801 ccgccgcgtgctaatctggtggcaccttcgcgcctgtctcgctgctttcgctaagtctctagccatttaaaatttttgataaccagctgcgacgcttttt
BglII (933)
BspLU11I (1000)
901 ttctggcgagatagtcttgtaaatgcgggccaagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcgtcccagcgca
1001 catgttcggcgaggcggggcctgcgagcgcggccaccgagaatcggacgggggtagtctcaaactggccggcctgctctggtgcctggcctcgcgccgcc
SacI (1195)
1101 gtgtatcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccgcttcccggccctgctgcagggagctcaaaa
SmaI (1217)
1201 tggaggacgcggcgcccgggagagcgggcgggtgagtcacccacacaaaggaaaagggcctttccttcctcatccgtcgcttcatgtgactccacggagt
XhoI (1332)
1301 accgggcgccgtccaggcacctcgattagttctcgagcttttggagtacgtcgtctttaggttggggggaggggttttatgcgatggagtttccccacac
1401 tgagtgggtggagactgaagagttaggccagcttggcacttgatgtaattctccttggaatttgccctttttgagtttggatcttgcctcattctcaagc
NcoI (1567)
AgeI (1559)
1501 ctcagacagtggttcaaagtttttttcttccatttcagGTGTCGTGAAAACTACCCCTACCGGTCACCATGGTTTCTAAGGGAGAAGAACTCTTTACTGG
1 Me t Va l Se r Ly s G l yG l uG l uLeuPh eTh r G l
1601 TGTTGTCCCAATTCTGGTTGAGCTGGATGGTGATGTGAATGGCCACAAATTCTCTGTGTCTGGTGAAGGTGAAGGAGATGCAACTTATGGAAAGCTGACT
1 1 yVa l Va l P r o I l eLeuVa l G l uLeuAs pG l yAs pVa l As nG l yH i s Ly s Ph eSe r Va l Se r G l yG l uG l yG l uG l yAs pA l a Th r T y r G l yL y s LeuTh r
1701
45
1801
78
1901
111
2001
145
2101
178
Acc65I (1789)
CTGAAGTTCATTTGTACAACAGGAAAGCTGCCAGTGCCTTGGCCAACTCTGGTGACCACCCTGACTTATGGTGTTCAATGTTTCAGCAGGTACCCTGACC
LeuLy s Ph e I l eCys Th r Th r G l yL y s LeuP r oVa l P r oT r pP r oTh r LeuVa l Th r Th r LeuTh r T y r G l yVa l G l nCys Ph eSe r A r gT y r P r oAs pH
ACATGAAGCAGCATGACTTCTTTAAATCTGCAATGCCAGAAGGTTATGTTCAGGAGAGGACAATCTTCTTTAAGGATGATGGAAATTATAAGACAAGGGC
i s Me t Ly s G l nHi sAs pPh ePh eLy s Se r A l a Me t P r oG l uG l y T y r Va l G l nG l uAr gTh r I l ePh ePh eLysAs pAs pG l yAs nT y r Ly s Th r A r gA l
AGAAGTGAAGTTTGAAGGTGATACACTGGTTAACAGAATTGAGCTGAAAGGCATTGATTTTAAGGAAGATGGAAACATTCTGGGTCACAAGCTGGAGTAC
a G l uVa l Ly s Ph eG l uG l yAs pTh r LeuVa l As nAr g I l eG l uLeuLy s G l y I l eAs pPh eLy s G l uAs pG l yAs n I l eLeuG l yH i s Ly s LeuG l uT y r
AACTATAATTCTCACAATGTTTACATTATGGCAGATAAGCAGAGGAATGGAATTAAGGCTAATTTCAAGATTAGACACAACATTGAGGATGGATCTGTCC
As nTyrAs nSe r Hi sAs nVa l T y r I l eMe tA l aAs pLy s G l nAr gAs nG l y I l eLysA l aAs nPh eLy s I l eAr gHi sAs n I l eG l uAs pG l ySe r Va l G
AACTGGCAGACCATTACCAGCAGAACACCCCTATTGGTGATGGCCCAGTTCTCCTCCCAGATAATCACTATCTCAGCACTCAATCTGCTCTGTCCAAAGA
l nLeuA l aAs pHi s T y r G l nG l nAs nTh r P r o I l eG l yAs pG l y P r oVa l LeuLeuP r oAs pAs nHi s T y r LeuSe r Th r G l nSe r A l a LeuSe r LysAs
NheI (2290)
2201 CCCTAATGAGAAAAGAGACCACATGGTCCTCCTGGAGTTTGTGACAGCAGCAGGAATTACTCTGGGAATGGATGAGCTGTACAAGTAAAGCTAGCTGGCC
211 pP r oAs nG l uLy s A r gAs pHi s Me t Va l LeuLeuG l uPh eVa l Th r A l aA l a G l y I l eTh r LeuG l yMe tAs pG l uLeuT y r Ly s •••
2301 AGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTT
2401 GTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGT
2501 AAAACCTCTACAAATGTGGTATGGAATTAATTCTAAAATACAGCATAGCAAAACTTTAACCTCCAAATCAAGCCTCTACTTGAATCCTTTTCTGAGGGAT
2601
2701
2801
2901
3001
105
3101
72
3201
38
3301
5
3401
GAATAAGGCATAGGCATCAGGGGCTGTTGCCAATGTGCATTAGCTGTTTGCAGCCTCACCTTCTTTCATGGAGTTTAAGATATAGTGTATTTTCCCAAGG
TTTGAACTAGCTCTTCATTTCTTTATGTTTTAAATGCACTGACCTCCCACATTCCCTTTTTAGTAAAATATTCAGAAATAATTTAAATACATCATTGCAA
TGAAAATAAATGTTTTTTATTAGGCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTTTA
ATAGAAATTGGACAGCAAGAAAGCGAGCTTCTAGCTTATCCTCAGTCCTGCTCCTCTGCCACAAAGTGCACGCAGTTGCCGGCCGGGTCGCGCAGGGCGA
125 •••As pG l nG l uG l uA l a Va l Ph eHi s Va l CysAs nG l yA l a P r oAs pAr gLeuA l a Ph
ACTCCCGCCCCCACGGCTGCTCGCCGATCTCGGTCATGGCCGGCCCGGAGGCGTCCCGGAAGTTCGTGGACACGACCTCCGACCACTCGGCGTACAGCTC
eG l uAr gG l y T r pP r oG l nG l uG l y I l eG l uTh r Me tA l a P r oG l ySe r A l aAs pAr gPh eAs nTh r Se r Va l Va l G l uSe r T r pG l uA l a T y r LeuG l u
GTCCAGGCCGCGCACCCACACCCAGGCCAGGGTGTTGTCCGGCACCACCTGGTCCTGGACCGCGCTGATGAACAGGGTCACGTCGTCCCGGACCACACCG
As pLeuG l yA r gVa l T r pVa l T r pA l a LeuTh r A s nAs pP r oVa l Va l G l nAs pG l nVa l A l a Se r I l ePh eLeuTh r Va l As pAs pAr gVa l Va l G l yA
SmaI (3225)
BssHII (3275)
GCGAAGTCGTCCTCCACGAAGTCCCGGGAGAACCCGAGCCGGTCGGTCCAGAACTCGACCGCTCCGGCGACGTCGCGCGCGGTGAGCACCGGAACGGCAC
l a Ph eAs pAs pG l uVa l Ph eAs pAr gSe r Ph eG l yL euAr gAs pTh r T r pPh eG l uVa l A l a G l yA l a Va l As pAr gA l a Th r LeuVa l P r oVa l A l a Se
TGGTCAACTTGGCCATGATGGCTCCTCCTGTCAGGAGAGGAAAGAGAAGAAGGTTAGTACAATTGCTATAGTGAGTTGTATTATACTATGCAGATATACT
r Th r LeuLysA l a Me t
BspLU11I (3446)
ATGCCAATGATTAATTGTCAAACTAGGGCTGCAGGTT AATTAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCT
3501 GGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCG
3601 TTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC
3701 AT AGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATC
3801 CGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGT
3901 GCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG
4001 TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGA
4101 TCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGGCTAGTTAATTAACATTTAAATC A