λ µ % ' ( # ) # *# * . ) % & ν∝ + , µ #$ #$ !" ' & µ % & ν % & ν * - *# # # # % /0 % ; 1 4 1 5 5 4 1 5 5 5 9 9 % ( % ν3 ( < < ' 233 2 6 7 86 3: 3 * ( . ( ( ν2 E / ' % " !" * = ( !" " " " " " " " " " ' ; 4 4 4 4 5 1 4 4 4 5 ; 4 9 9 9 4 4 4 < / ' ' . ' . + 6 :8 3 3: 338 7 :8 2 8 *5= 5> = % !#" 4!? 1 = % @ > *5@A 4 @ > B5@! C ? *!4 > *5= 5> = % !#" 4!? 1 = % @ > *5@A 4 @ > B5@! C ? *!4 > D ' ( 452 * /0 545 3 : ( 3 F8 ( 452 925 ' * ( ( %+ G ( H + D espectro infrarrojo del aire H2O modo ν2 CO2 modo ν3 CO2 modo ν2 640 660 680 500 H2O modo ν1 700 1000 1500 2000 2500 3000 3500 H2O modo ν3 4000 -1 número de onda (cm ) !" + D 452 C 5 , + 12 ' 452 . absorbancia 4 0 630 650 670 690 núme ro de onda (cm-1) 710 absorbancia 5 0 2300 / ' 2320 2340 2360 número de onda (cm-1) ( 5&4 1 9 " 9 4 5 2380 1 " Vibraciones típicas en péptidos y proteínas Designation Approximate frecuency (cm-1) Nature of vibration A B 3300 3110 N-H stretching, in resonance with overtone (2 x amide II) I 1653 II 1567 III 1299 IV 627 40% O=C-N bending; 60% other V 725 N-H bending VI 600 C=O bending VII 200 C-N torsion 80% C=O stretching; 10% C-N stretching; 10% N-H bending 60% N-H bending; 40% C-N stretching 30% C-N stretching; 30% N-H bending; 10% C=O stretching; 10%O=C-N bending; 20% other 2 %. > % ' # !" . 5&4&5 &3 5&4&5 3% # I% &2 3% 4 # & %& ' % # . - 2 % 2% % 4 5 5 4 &3 2% + J 4 &I # 4 &2 % ' !" + >&4&> 4 & # % 4 > > 4 > 4 & > & # 4 4 I4 9 . 493 492 I493 % K 934 59 4 4 4 9 4 4 ' 493 493 492 84559 5 9 4 9 5 9 493 4 5 4 9 4&5 4 5 E / = ; + 4 5 9 ' ' 3 % ' " 4 4 * / % '0 B / 4 9 '0 1 "*1 !& L ' 4&4 2 M M & ! !% '0 E / % ! + '0 4 5 9 ' ' 59 % % /0 ' / '0 # B / % !& . ' / % ' . # > %2 ∆% & %2 ' '0 "*1 ' 65 29 ' ∆% I1 !& ' B % O 3I> 5 !& N2 !&8N2 ' # ' 24 ' ↑↑ 2 <<< & N2 /0 ∆% & / ) 4 ' ⋅P ⋅# 4 > &- N2 & N2 ↑ % 4 5 '0 /0 ' > ↑ !& N2 3N2 <<< 4&4 D ↑ 4 9 ↑↓ 4 + 5 9 E > !& N2 # > ' ∆% ' ↑ . & - N2 → ' ν ↑ & N2 , + & P ⋅# B 9 34 #& C ν & I2<: *9, ν & <6 *9, &- N2 4 ' ' / + # "*1 @ ' ' % . ' ' Q J ' ' / . ' / ' # 4 σ # ' J 0 + . ' + G H # +&#⋅ ν& ( J Fσ ' 9 4 4 9 ' . # ' P ⋅#⋅ . Fσ I ' E + 493 934 > ν ' 4 34 9 ν& P ⋅#⋅ ' , J 0 ' , ( J > ' σ σ / ν−ν ν : J ( ' , 4 9α 4 9β 4 D ' J / = ' , = J 0 ' , J 0 5 9 - 9γ R D ' J R / 4 J 0 ' J 0 9 4 9 ' ' , R 9 9 4 9 = / δO '' "*1 = = / / E Fσ ' ' J Fσ & P ⋅#⋅ J δ= 4 S 0 ' J 0 C *> 493 493 B J ( ' , / J 8