Revista Latinoamericana de Química

REVISTA
LATINOAMERICANA DE QUÍMICA
EDITOR
Dr. Andrés Navarrete
The Revista Latinoamericana de Química is an international Journal. All manuscripts
submitted are subject to peer review. Expert scientists in the subject areas being treated
will evaluate all manuscripts for validity of all experimental procedure, originality,
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La Revista Latinoamericana de Química es una publicación internacional. Todos los manuscritos
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los manuscritos en cuanto a la validez de los procedimientos experimentales, originalidad,
importancia y adecuación para la Revista.
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Revista Editada por:
Laboratorios Mixim, S.A. de C.V.
Calle Jardín Sur No. 6, Apartado Postal 3,
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Science Library Services, entre otras.
MÉXICO 2013
29 a 31 de Mayo de 2013
Universidad Autónoma del Estado de Hidalgo
Pachuca de Soto, Hidalgo
México
I
Maestro Humberto Augusto Veras Godoy
RECTOR DE LA UNIVERSIDAD AUTÓNOMA DEL ESTADO DE HIDALGO
Dr. Orlando Ávila Posos
DIRECTOR DEL INSTITUTO DE CIENCIAS BÁSICAS E INGENIERÍA, UAEH
Dr. Carlos Andrés Galán Vidal
JEFE DEL ÁREA ACADÉMICA DE QUÍMICA, ICBI, UAEH
Dra. Noemí H. Waksman Minsky
PRESIDENTA DE LA ASOCIACIÓN MEXICANA DE INVESTIGACIÓN EN PRODUCTOS
NATURALES, A.C.
COMITÉ ORGANIZADOR
COMITÉ CIENTÍFICO
Dr. J. Martín Torres Valencia
Dr. J. Roberto Villagómez Ibarra
Dr. Alejandro Álvarez Hernández
Dr. Oscar R. Suárez Castillo
Dra. Myriam Meléndez Rodríguez
Dra. Maricruz Sánchez Zavala
Dra. Susana Rojas Lima
Dr. Heraclio López Ruiz
Dra. Margarita Tlahuextl Romero
Dr. Antonio R. Tapia Benavides
Dr. Pedro Joseph-Nathan
Dr. Carlos M. Cerda García Rojas
Dra. Maricruz Sánchez Zavala
M.C. Dora luz Quintero Mogica
M.C. Araceli Sierra Zenteno
Dra. Minarda de la O Arciniega
Dr. Juan A. Gayosso De Lucio
Dra. Mirandeli Bautista Ávila
Dra. Georgina Almaguer Vargas
Dra. Raquel Cariño Cortés
Dra. Claudia Velázquez González
Dr. Eleuterio Burgueño Tapia
Dr. Juan D. Hernández Hernández
Dra. Beatriz Hernández Carlos
Dra. Guadalupe Hernández Linares
Dra. María A. Fernández Herrera
Dra. Noemí H. Waksman Minsky
Dra. Verónica M. Rivas Galindo
Dra. Maria Luisa Garduño Ramírez
Dra. María de la Luz Miranda Beltrán
Dra. Ana María Puebla Pérez
Dr. Ramón E. Robles Zepeda
Dr. Sergio E. Peraza Sánchez
COMITÉ DE LOGÍSTICA
Q. L. Adriana Ortiz León
M.C. Alejandro Valdez Calderón
M.C. René Velázquez Jiménez
Q. Manuel Rodríguez Villanueva
Q. Ma. Guadalupe Vázquez O.
Ing. Q. Martha Helena Hernández
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El mango (Mangifera indica (MI)) es un cultivo de clima tropical y subtropical, considerado como una de las
especies de mayor importancia económica, la pulpa del fruto se utiliza para la elaboración de jugos, néctares,
conservas, etc., mientras que los residuos (cáscara, semilla y pulpa) no son aprovechados, debido a la ausencia de
evidencias experimentales respecto a su valor nutricional. El objetivo de este trabajo fue determinar la
bioaccesibilidad intestinal de compuestos antioxidantes y la capacidad antioxidante presente en tres productos de MI:
el extracto etanólico de la hoja (EH), el residuo industrial (cáscara, semilla y pulpa) obtenido del despulpado (RD) y
el RD secado por aspersión (RDA). La bioaccesibilidad intestinal se evaluó por un proceso enzimático digestivo in
vitro seguido por un proceso de diálisis. La actividad antirradical fue cuantificada mediante los ensayos con DPPH•
y ABTS.+. Los resultados indicaron una concentración de polifenoles extraibles de 196.67, 136.41 y 584.79 mg
EAG/g en las muestras de RD, RDA y EH en la muestra original, respectivamente. Estos valores son similares a lo
presentado en otros estudios en frutos (Ribeiro et al, 2008; Kunradi VFG et al. 2009). Después del proceso digestivo
el contenido de polifenoles totales fue menor (141.014, 68.188 y 342.391mgEAG/g de muestra en RD, RDA y EH),
sin embargo representan entre 50 y 70 % de los compuestos presentes en la muestra original, siendo similar a lo
encontrado en otros trabajos (Ramírez-Moreno, 2011). Estos compuestos liberados y bioaccesibles a nivel intestinal
se debe a que son liberados por la acción enzimática gastrointestinal, ya que se hidrolizan los enlaces no covalentes
entre los grupos hidroxilo de los polifenoles y los grupos polares de las moléculas polisacaridas (González AGA. et
al.; Hugo PC, 2011). Estos compuestos liberados por el proceso digestivo mantuvieron en gran medida la actividad
antirradical medida por DPPH, en el RD y RDA (86.70 y 87.78 %), mientras que para la hoja se observa un
porcentaje menor de actividad (30.64%). En el caso del ABTS, estos valores de bioaccesibilidad fueron incluso
mayores. La muestra original presento valores de (79.17, 78 y 79.33 mgAA/g) y después del proceso de digestión se
encontraron valores de 1730.83, 742.91 y 1878.88 mgAA/g para RD, RDA y EH, respectivamente. Dicha actividad
antioxidante puede atribuirse a la liberación de compuestos antioxidantes como polifenoles (Ribeiro et al, 2008), Dtocoferoles, carotenoides, fosfolípidos presentes en semilla y cascara del mango (Soong YY et al, 2004; Patricia
Morales et al, 2012). Estos compuestos antioxidantes que llegan a sangre aportaran un buen porcentaje de actividad
antioxidante. Por otra parte los compuestos no digeridos se asociaron a la fracción indigestible de la muestra,
indicándonos que llegan a intestino grueso en donde aporta un ambiente antioxidante. La importancia de este estudio
radica en que es la primera contribución de bioaccesibilidad intestinal de los compuestos bioactivos procedentes de
los RD, RDA y EH del mango. Finalmente, de acuerdo a los resultados encontrados dichos extractos podrían ser
utilizados con fines funcionales en diversas patologías, así como ingrediente bioactivo de los alimentos.
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