Dr. Elder de la Rosa Cruz Sinodales

Asesor:
Dr. Elder de la Rosa Cruz
Sinodales:
Dr. Pedro Salas Castillo
(Sinodal Externo – CFATA UNAM, Secretario)
Dr. Claudio Frausto Reyes
(Sinodal Interno, Vocal)
Dr. Elder de la Rosa Cruz
(Asesor de Tesis, Presidente)
Tesis:
“BIOLOGICAL APPLICATION OF CERAMIC AND METALLIC NANOPARTICLES
BY EXCITATION IN THE NEAR INFRA-RED”
Resumen:
In this thesis, nanoparticle of zirconium oxide doped with ytterbium and erbium (2% and 1%, respectively) and
gold, which have interesting optical properties for use in bio-applications, such as up-conversion and
luminescence by two-photon absorption, in which excitation occurs in the near infrared. For labeling of HeLa
cells, the nanoparticles of ZrO2: Yb3+, Er3+ were conjugated with biotin and anti-Ki67, later incubated in these
cells having obtained promising results due to the strong red emission of these nanoparticles in addition that
had not made the marking cells with this type of material. Moreover gold nanoparticles, synthesized by the
Turkevich method, were used as markers of Cervical tissue out to be a good option for marking due to its ability
to present luminescence two-photon resulting in a good choice to image biological samples, as well that the
analysis of the tissues was being enhanced raman spectroscopy signal, being able to observe preliminary results
for the study of differences between healthy and carcinoma tissue. From these spherical particles by using them
as seeds, multi-branched gold nanostructures (MBGNs) were obtained, with non-surfactant to which
subsequently were pladded a silica-coating to improve stability, later when had controlled synthesis of
nanostructures, MBGNs were proven to determine its effectiveness in Raman spectroscopy as substrates for
enhanced signals (SERS), for which we could obtain significant results since it was detected Rhodamine B and αglucose in liquid at concentrations of 50 pM and 5 mM ( 90 mg dL-1) respectively with a laser exciting 785 nm.