1290 Infinity LC

Possibilitats Instrumentació actual HPLC.
1220 Infinity
1260 Infinity
Marisa Lleberia
[email protected]
Account Manager
1290 Infinity
The Agilent 1200 Infinity Series HPLC
1200 Infinity
Series
1260
Infinity
1220
Infinity
1290
Infinity
PrepScale LC
Low-flow
Analytical flow
Prep-flow
LowFlow LC
Isocratic,
manual
Cap.LC
Binary
Nano-LC
Gradient,
manual
Binary
Binary-SFC
Chip-LC
Gradient,
automated
Quaternary
Quaternary
Bio-Inert
Isocratic
Page 2
Isocratic
Binary
Quaternary
Agilent 1200 Infinity Series HPLC
Raising the Standard in HPLC – UHPLC for EVERY Lab
1220
Infinity LC
1260
Infinity LC
1290
Infinity LC
Integracromatográfique
s ted
600 bar, 5ml/min
80 Hz
Modular
600 bar, 5ml/min
80 Hz
Modular
1200 bar, 5ml/min
160 Hz
2x or 10x UV Sensitivity
100% HPLC Compatible
HPLC Price – UHPLC Capability
1st Universal LC by ISET
Highest Performance
HPLC Service Costs
Most affordable
HPLC and UHPLC
1260/1290 Technology
Page 3
Instrument  Column Compatibility
Column Type
HPLC
UHPLC
UHPLC
Particle
3 – 5um
< 2um (STM)
Superficial Porous
Column Length / mm
50 - 300
CoIumn I.D. / mm
3.0 - 4.6
2.1
3.0
4.6
2.1
3.0
4.6
2.1
3.0 - 4.6
Max Pressure / bar
400
1200
1200
600
1200
1200
600
600
600
Agilent Column type
Short: 30 – 50
Long: 100 – 150
30 - 150
Various RRHD RRHD RRHT RRHD RRHD RRHT Poroshell Poroshell
1290B/Q – 1200bar
best
1260B – 600bar
good
2)
1260Q/1220G – 600bar
1)
Conventional LC 400bar
2)
2)
2)
2)
accept.
1)
2)
3)
3)
compr.
Any column of any dimension and particle can be used on 1220/1260/1290 Infinity LC‘s
1200 Infinity Series matches UHPLC columns pressure rating:
• 1290 Infinity LC: 1200bar + lowest delay volume (DV) for optimum support of 2.1 - 4.6mm ID STM (RRHD)
• 1260/1220 Infinity LC: 600bar + std DV for optimum support of 3 - 4.6mm ID STM (RRHT) & SP (Poroshell)
STM-short: sub-2µm particles, 30 – 50mm length
STM-long: sub-2µm particles, 100 – 150mm length
Page
4
HPLC:
3 - 5 µm particles
1) Because of delay volume.
2) Because of delay volume and pressure.
3) Because of pressure.
The Agilent 1200 Infinity Series
- Power range
 All analytical columns
pressure
 Method Transfer
1200
 Higher Resolution
 Faster Analysis
1000
 More viscous solvents
1290 Infinity LC
800
 Lower temperatures
 Semi-prep
600
400
1260 Infinity Binary LC
1260 Infinity Quat./Iso LC
1220 Infinity LC
200
0
0
Page 5
2
4
6
8
10
flow
From HPLC to RRLC on the 1220 Infinity LC
by Varying Particle Size and Column Length
mAU
Flow rate: 1.2 mL/min
at 40°C
140
6
From HPLC to RRLC
120
100
3 x 50, 1.8 µm, Tg = 3 min
Resolution peak 6 = 1.09
Run time = 3.2 min
80
3 x 100, 1.8 µm, Tg = 6 min
Resolution peak 6 = 1.7
Run time = 5.7 min
60
40
3 x 100, 3.5 µm, Tg = 6 min
Resolution peak 6 = 1.29
Run time = 5.5 min
20
3 x 150, 5 µm, Tg = 9 min
Resolution peak 6 = 1.23
Run time = 7.5 min
0
1
2
3
4
5
6
7
min
Increasing Flow Rate for Shorter Analysis Time
with Improved Resolution on Agilent 1220 Infinity
LC
3x50mm, 1.8µm column
at 1.2mL/min at 315bar,
40°C, 80Hz
Resolution for peak 6=
1.09
VWD1 A, Wavelength=254 nm (TEST1220\PART14000001.D)
mAU
70
6
60
50
40
30
20
10
0
0
0.5
1
VWD1 A, Wavelength=254 nm (TEST1220\PART21000001.D)
1.5
2
2.5
mAU
50
min
3x50mm, 1.8µm column
at 2.4mL/min at 510bar,
50°C, 80Hz
Resolution for peak 6 =
2.53
6
60
3
40
30
20
10
0
0
0.5
1
1.5
2
2.5
3
min
Increase flow rate and adapt gradient time and temperature
1220 Infinity LC
Isocratic and Gradient RRLC at most affordable Price
Most affordable access to 1260 Infinity Quality & Performance
• Available flavors:
• 1220 Infinity manual isocratic system (upgraedable)
• 1220 Infinity manual binary gradient system (upgradeable)
• 1220 Infinity automated binary gradient system
1220
Infinity LC
Integracromatográfique
s ted
600 bar, 5ml/min
80 Hz
Most affordable
HPLC and UHPLC
1260/1290 Technology
Page 8
• Productivity: 10x higher than conventional HPLC
systems
• 600 bar up to 5 mL/min (10 mL/min at 200 bar)
• 80 Hz detector speed
• Technology and Quality: 1260 and 1290 Infinity
inside!
•
•
•
•
Degassing: Same Technology/Parts as 1290 Bin/1260 Quat degasser
Pump: Same Technology/Parts as 1260 Quat Pump (2-channel)
Autosampler: Same Technology/Parts as 1260 ALS (no cooling)
VWD: Same Technology/Parts as 1260/1290 VWD
• Upgrade capabilities
1260 Quaternary LC System
Enhanced Quaternary & Binary RRLC at Quaternary HPLC
• Productivity: 10x higher than 1200/1100
Quaternary HPLC
• 600 bar up to 5 mL/min (10 mL/min at 200 bar)
• 80 Hz Detector Speed
* ) 60,000
systems sold!
• Sensitivity:
1260
Infinity LC
Modular
600 bar, 5ml/min
80 Hz
2x or 10x UV Sensitivity
100% HPLC Compatible
HPLC Price – UHPLC
Capability
Page 9
• Up to 10x higher than 1200/1100 Quaternary HPLC
• 10x by new 1260 Infinity DAD with 6 cm flow cell (± 0.6 µAU)
• 2x by new 1260 Infinity VWD and 1260 Infinity DAD with 1cm flow
cell
• Technology: Leverage of 1290 Infinity LC
innovations
• Pump: New, build-in degassing unit and next generation inlet
valves
• Detectors: Next generation VWD and DAD-technology
• Price: Similar as 1200 Series Quat (400 bar, 20
Hz, ± 6 µAU )
1260 Infinity Bio-inert HPLC
I
100% Bio-inert
• Metal-free sample flow path (instrument and column)
• Stainless steel–free solvent flow path
• Widest pH range (1-13, short-term 14)
600bar power range
• From lowest pressure up to highest pressure STM
columns
Semi prep 10ml/min
When is this instrument recommended??
Infinitely Better for Bio-molecule Analysis
Page 10
1260 Infinity Bio-inert HPLC
What´s new compared to our standard
1260 Infinity Quaternary LC
 Pump titanium based, no corrosion
Active Seal included.
Autosampler metal-free
 Capillaries and connections metal-free
 Detector Flow Cell metal-free
pH-range 1-13, shortterm pH 14
Advantatges:
•Wide pH range
• High salt tolerance
• Lowest surface activity
600 bar (UHPLC) now
 Bio-Inertness, no passivation
also for fast or high
resolution Bioseparations needed
11
Target Applications
Biologic Characterization
Reverse Phase:
Separation of Intact Biologics/Variants
Size Exclusion: Aggregate Analysis
12
Ion Exchange: Separation of Isoforms
Peptide Mapping:
Post-translational modifications
1290 Infinity LC
More Affordable Access to the World‘s Best UHPLC
UHPLC Productivity with HPLC Service Costs
• Service cost similar to standard HPLC, attractive upfront
contracts
• Based on
– proven robustness – 2x higher than 1100 Series at introduction
– Increased parts lifetime / longer maintenance intervalls
1290
Infinity LC
Modular
1200 bar, 5ml/min
160 Hz
– new design-for-supportability technology for faster maintenance,
diagnostics and repair
• Technology:
– 1200 bar system pressure for pump, autosampler and valves to
match any sophisticated application (method development solution).
– Highest detector sensitivity on the market (0.6 µAU/cm noise level)
– Lowest autosampler carry-over (<0.001 %, <10 ppm for UV and
MS detection in combination with 1290 Infinity Flexible Cube)
1st Universal LC by ISET – New high-capacity solution for highest throughput analyses
Highest Performance
HPLC Service Costs
Page 13
Bombas UHPLC 1290 Infinity
Robustez y alta capacidad de disipación de calor
Nuevo material del pistón: SSiC*
(Carburo de silicio sintético).
Proporciona Mejor Conductividad térmica y menor
fricción para menor desgaste.
Permite una optima disipación de calor
• Mayor presión x flujo (1200bar at 2 ml/min)
• Alarga el tiempo de vida de los sellos/ intervalos de
mantenimiento (>150L)
• Un
solo sello
paracerámico
fase normal
SSiC*:
Material
de alta y reversa
dureza formado por Carburo de Silicio
sintético.
Volumen Embolada variable (patentado) 
menor desgaste y mejores
prestaciones.
(1050-1100-1200-1260-1290)
Capilares
Multi-Capa para un óptimo
intercambio de calor entre los
cabezales de las bombas.
1290 Infinity LC – Delay Volume and Mixing Noise
- How to achieve lowest delay volume, lowest noise and highest RT precision?
1200 Series
Solvent A
1200 Series
Solvent B
Passive Damper
180-580 µl
Passive Mixer
400 µl
Very Good Noise
~ 800 µl
1290 Inifinity
Solvent A
1290 Inifinity
Solvent B
5 µl / 40µl DV:
Active Damping
< 5µl
+
Microfluidic Mixing
35µl
 80x / 20x lower
=
Lowest Noise
 < 50%
Page 15
1290 Infinity Binary Pump
Amortiguación electrónica de pulsos
Motor del Pistón
• Motores independientes
OF
O
F
N
HPLC
1200
para cada pistón-> minimas
oscilaciones en la linea de base
• Pasos de 300 picolitros
1290
Inifinity
1290
Chanel
UHPLC
1290
Inifinity
A
Chanel
B
Page 16
Agilent Jet Weaver: Nuevo sistema de mezclado
Mezclador de alta eficacia.
PLACA ACERO INOXIDABLE CON MICROCANALES DE 35µl de volumen
La mas alta eficiencia de mezcla con el mínimo
volumen para un bajo nivel de ruido
• V=35 µl, para aplicaciones UV estándar.
Tecnología:
• Tecnología Propietaria de Microfluidos Multi-capa.
• Bandas de difusión de acero con estructuras de
(100x120µm)
Page 17
1290 Infinity Compartimento de columnas termost.
Siguiente nivel en facilidad de uso:
New leak funnel
New capillary guide
New 2PS/6PT, 2PT/10PT and 9PT/8PS QuickChange high pressure valves
Page 18
1290 Infinity Diode Array Detector
- How the optics work
Mirror
Grating
Programmable
slit
Deuterium lamp
Max-Light cartridge cell
1024 element
diode-array
Max-Light Cartridge Cell
- Optofluidic waveguides
Non-coated fiber (fused silica)
High Light Transmission due to Total-Internal Reflection (TIR) principle
(~ 100 % Light efficiency)
Optofluidic Waveguides
• Long path length
• Small cell volume
• High light transmission
Benefits:
 Highest Sensitivity (S/N) with small cell volumes (dispersion effects)
 More reliable and robust peak integration (automated) due to
nearly no Refractive Index and thermal effects (solvent temperature)
Cartridge design for ease of use
Page 20
Highest S/N
1290 Infinity Diode Array Detector
- Baseline robustness due to significant reduced RI effects
1200 RRLC System (no mixer, damper)
Flow rate: 0.5 ml/min (5-95 % ACN)
Temp: 80 °C, Data rate: 40 Hz, 230 nm
with Reference Signal (360 nm)
1290
Infinity
DAD
1200 DAD
SL
Page 21
Next Level Sensitivity and Productivity
 Automated integration at any condition
 2 – 20x higher sens with 1 cm Cell
 8 – 80x higher sens with 6 cm UHS Cell
11.4 x Higher Sensitivity
-1290 Infinity DAD compare to 1200 Series DAD SL
Columns: 150 x 4.6mm Zorbax SB C18, 5µm
Sample: Anthracene: 835 pg/µL
Mobile phase: A: Water, B: Acetonitrile
Elution: isocratic 80 % B
Injection volume: 5 µL
Flow: 1.5 mL/min
DAD: 251/4nm, Ref= 450/80nm,
2.5Hz, slit width 4nm
mAU
30
25
1290 DAD
60 mm
20
1290 Infinity DAD
15
10
0
0
22
Height [mAU]
28.87579
4.93845
Noise [mAU]
0.009806
0.01908
Signal/ noise
2944
259
Sensitivity
increase
+11.4
1200 Series DAD SL
5
1
2
3
4
5
min
1200 DAD SL
10mm
1290 Infinity Diode-Array Detector
- Programmable slit
Absorbance
(mAU)
0.6
0.5
0.4
0.3
0.2
0.1
0
230 240 250 260 270 280 nm
1 nm
Noise level
2 nm
4 nm
8 nm
Programmable slit for easy optimization of sensitivity,
linearity and spectral resolution
Page 23
Instrument to Instrument Method Transferability
- Impact of delay volume and mixing behavior
• What happens with a programmed gradient?
response
90%
Programmed gradient step
1200 Series Binary Pump SL
1290 Infinity Binary Pump
Gradient slope/
Mixing behavior
10%
Delay
volume
1200 Series
1290 Infinity
Page 24
time
Total delay volume of the system
(sum of capillaries, mixer, cells,
valves..)
NEW
Intelligent System
Emulation Technology
(ISET)
The Solution
Page 25
Intelligent System emulation technology
- How it works (1290 to 1200)
mAU
400
350
300
Injection
Same gradient conditions
by moving the gradient and
by emulating the gradient
mixing behavior.
250
200
150
Programmed gradient
100
1290 gradient
50
1200 gradient
0
0
Page 26
0.5
1
1.5
2
2.5
3
3.5
min
Method Transferability: 1290 Infinity LC
- System Emulation Technology
Concept
FA
Emulated
System A
Select System to be emulated by a simple mouse click:
Agilent
1100 Series
Page 27
Agilent 1200
Series LC
Agilent 1260
Infinity LC
Agilent 1220
Infinity LC
Alliance
Method transfer, pesticide example
2.1x100 mm Zorbax Eclipse Plus, 1.8 µm column, flow: 0,8 mL/min
(A)
1100
Quaternary
400 bar
(B)
1290 Infinity
1 200bar
(C)
1290 Infinity
1200 bar
with ISET
Page 28
1
2,3
4
5
6,7
8
9 10,11,12 13 14 15
16
Agilent´s Analytical LC Portfolio
Compatible and Scaleable across entire Series
1290
Infinity LC
1260/1220
Infinity LC
Methods compatibility
 Run 1100/1200 Series
Methods on 1260/1290
 Modules Upgrade
1000/1100
Infinity LC
1100/1200 Series with
1260/1290 Infinity
Page 29