Franc Llorens DNI: 39884273L Date of Birth: 05/17/1977 Phone number: 654703038 e-mail: [email protected] EDUCATION: 2005: PhD Degree in Biochemistry and Molecular Biology obtained in the Universitat Autònoma de Barcelona (UAB) 2002: DEA Degree in Biochemistry and Molecular Biology obtained in the Universitat Autònoma de Barcelona (UAB) 1999: B.Sc Degree in Biochemistry obtained in the Universitat Autònoma de Barcelona (UAB) PROFESSIONAL EXPERIENCE: Since 2012: Postdoctoral Researcher: Bellvitge Biomedical Research Institute (IDIBELL) Institute of Neuropathology University Hospital of Bellvitge (HUB) 2008-2011: Postdoctoral Researcher: Institute for Bioengineering of Catalonia (IBEC) Barcelona Science Park Program: Cellular Biotechnology Unit: Molecular and Cellular Neurobiotechnology 2005-2008: Postdoctoral Researcher: Centre for Genomic Regulation (Barcelona Biomedical Research Park) Program: Bioinformatics and Genomics Program. Unit: Genomic Analysis of Development and Diseases 2003-2005: Associated Professor Department of Biochemistry and Molecular Biology Universitat Autònoma de Barcelona (UAB) 2000-2005: PhD: Department of Biochemistry and Molecular Biology Universitat Autònoma de Barcelona (UAB) 1998 Practical training Chemistry Laboratory - Vandellòs Nuclear Power Plant FELLOWSHIPS AND AWARDS: 2006-2008: Post-Doctoral Fellowship (Juan de la Cierva-Spanish Government) 2000-2003: PhD Fellowship (Generalitat de Catalunya) PROJECTS: TITLE: Interacción de la proteína quinasa CK2 con el factor de inicio de la traducción eIF-2 y la chaperona grp94. Caracterización de las regiones de interacción e importancia funcional en la respuesta a los factores de estrés. PB98-0856 FUNDING: MCYT FROM: 1999 TO: 2002 PRINCIPAL INVESTIGATOR: Emilio Itarte TITLE: Análisis estructural y funcional de la interacción de las proteínas GRP94, eIF-2 y la proteína quinasa CK2. HI2002-0022 FUNDING: Acción Integrada Hispano-Italiana MCYT FROM: 2003 TO: 2005 PRINCIPAL INVESTIGATOR: Maria Plana Coll TITLE: Proteína quinasa CK2 y vía RAF/MEK/ERK: Puntos de interconexión y su contribución a los efectos biológicos de fármacos antitumorales. SAF2002-03239 FUNDING: MCYT FROM: 2003 TO: 2006 PRINCIPAL INVESTIGATOR: Emilio Itarte TITLE: Interacción de la proteína quinasa CK2 con el factor de inicio de la traducción eIF-2 y la chaperona grp94. Caracterización de las regiones de interacción e importancia funcional en la respuesta a los factores de estrés. HI2002-0022 FUNDING: Acción Integrada Hispano-Italiana MCYT FROM: 2001 TO: 2005 PRINCIPAL INVESTIGATOR: Emilio Itarte TITLE: Grupo de Excelencia. SGR2001-00199 FUNDING: Generalitat de Catalunya FROM: 2001 TO: 2005 PRINCIPAL INVESTIGATOR: Pere Suau TITLE: Estudio genómico funcional del gen LRRN6A en la diferenciación neuronal. Optimización de metodologías experimentales y bioinformáticas para la aplicación de los microarrays a estudios de expresión. BFU2006-13651 FUNDING: MCYT FROM: 2005 TO: 2008 PRINCIPAL INVESTIGATOR: Lauro Sumoy TITULO DEL PROYERCTO: Centro de investigación neurodegenerativas FUNDING: CIBERNED FROM: 2009 TO: 2012 PRINCIPAL INVESTIGATOR: Jose Antonio del Río en red en enfermedades TITLE: Grupo de Excelencia. SGR2005-0336 FUNDING: Generalitat de Catalunya FROM: 2010 TO: 2014 PRINCIPAL INVESTIGATOR: Jose Antonio del Río TITLE: Caracterización funcional de genes regulados durante la ontogenia del SNC en el desarrollo cortical y la regeneración axonal. BFU2009-10848 FUNDING: MCYT FROM: 2010 TO: 2013 PRINCIPAL INVESTIGATOR: Jose Antonio del Río TITLE: FP7 EUROPEAN GRANT PRIORITY: Protecting the food chain of prions: Shaping European priorities through basic and applied research. KBBE-2007-2-4-06 Large scale Integrated Collaborative Project FUNDING: UE (FP7 – Seventh Framework Programme) FROM: 2009 TO: 2013 PRINCIPAL INVESTIGATOR: Jesús Requena PUBLICATIONS: 24. Cross-validation of mRNA and miRNA high-throughput expression data Llorens F#, Hummel M, Sumoy L. Manuscript in preparation 23. A functional miR-101 isomiR in human brain. Llorens F#, Bañez-Coronel, Del Río JA, Martí E. Manuscript in preparation 22. Multiple platform assessment of the EGF dependent microRNAome by microarray and deep tag sequencing analysis Llorens F#, Hummel M, Pastor X, Ferrer A, Mattlin H, Vivancos A, Bayés M, Himmelbauer H, Sumoy L. Manuscript in preparation 21. Unraveling the neuroprotective mechanisms of PrPc in neurotoxicity Llorens F#, Del Río JA. Prion 20. Enhanced proliferation in PrPc-deficient oligodendrocyte precursor cells during development and in the adult mouse CNS Bribián A, Fontana F*, Llorens F*, Gavín G, Reina R, Garcia-Verdugo JM, Clemente D, De Castro F, del Río JA. PLoS One 19. Neuroprotective role of PrPC to Kainate-induced hippocampal neurotoxicity depends on its interaction with GluR6/7 and PSD-95 and further JNK3 activation. Carulla P, Bribian A, Rangel A, Gavín R, Caelles C, del Río, JA. Llorens F#. Molecular Biology of the Cell, 2011, Jul 14 18. Multiple platform assessment of the EGF dependent transcriptome by microarray and deep tag sequencing analysis. Llorens F, Hummel M, Pastor X, Ferrer A et al. BMC Genomics, 2011, Jun 23; 12:326 17. Emerging functions of myelin associated proteins during development, neuronal plasticity and neurodegeneration Llorens F, Gil V, Del Río JA. FASEB J. 2011, 25(2):463-75 Review 16. Application of massively parallel sequencing to miRNA and IsomiR characterization and profiling in Huntington disease Martí E, Bañez-Coronel M, Pantano L, Llorens F, Ferrer I, Estivill X. Nucleic Acid Research, 2010, 38(20):7219-35 15. Regeneration of Cortical Connections by Gsk3β Inhibition Independently Of Nogo Receptor 1. Seira O, Gavín R, Gil V, Llorens F, Rangel A, Soriano E, del Río JA. Journal of Neurochemistry. 2010, 113(6):1644-58 14. Developmental Expression of the Oligodendrocyte Myelin Glycoprotein in the Mouse Telencephalon. Gil V, Bichler Z, Lee JK, Seira O, Llorens F, Bribian A, Morales R, Claverol-Tinture E, Soriano E, Sumoy L, Zheng B, Del Río JA. Cereb Cortex. 2009 Nov 5 13. Regulation of GABA(A) and Glutamate Receptor Expression, Synaptic Facilitation and Long-Term Potentiation in the Hippocampus of Prion Mutant Mice. Rangel A, Madroñal N, Massó AG, Gavín R, Llorens F, Sumoy L, Torres JM, Delgado-García JM, Río JA. PLoS One. 2009 4:e7592 12. Developmental analysis of Lingo-1/Lern1 protein expression in the mouse brain: interaction of its intracellular domain with Myt1l. Llorens F, Gil V, Iraola S, Carim-Todd L, Martí E, Estivill X, Soriano E, del Rio JA, Sumoy L. Dev Neurobiol. 2008 68(4):521-41 11. RCAN1 (DSCR1) increases neuronal susceptibility to oxidative stress: a potential pathogenic process in neurodegeneration. Porta S, Serra SA, Huch M, Valverde MA, Llorens F, Estivill X, Arbonés ML, Martí E. Hum Mol Genet. 2007 May 1;16(9):1039-50. 10. Proteomic analysis of SET-binding proteins. Vera J, Estanyol JM, Canela N, Llorens F, Agell N, Itarte E, Bachs O, Jaumot M. Proteomics. 2007 7(4):578-87. 9. Cross talk between protein kinase CK2 and eukaryotic translation initiation factor eIF2beta subunit. Llorens F, Sarno S, Sarró E, Duarri A, Roher N, Meggio F, Plana M, Pinna LA, Itarte E. Mol Cell Biochem. 2005 274(1-2):53-61. 8. The N-terminal domain of the human eIF2beta subunit and the CK2 phosphorylation sites are required for its function. Llorens F#, Duarri A, Sarró E, Roher N, Plana M, Itarte E. Biochem J. 2006 394(Pt 1):227-36. 7. Unbalanced activation of ERK1/2 and MEK1/2 in apigenin-induced HeLa cell death. Llorens F, Miró FA, Casañas A, Roher N, Garcia L, Plana M, Gómez N, Itarte E. Exp Cell Res. 2004 299(1):15-26. 6. Eukaryotic translation-initiation factor eIF2beta binds to protein kinase CK2: effects on CK2alpha activity. Llorens F, Roher N, Miró FA, Sarno S, Ruiz FX, Meggio F, Plana M, Pinna LA, Itarte E. Biochem J. 2003 375(Pt 3):623-31. 5. Persistent nuclear accumulation of protein kinase CK2 during the G1-phase of the cell cycle does not depend on the ERK1/2 pathway but requires active protein synthesis. Miro FA, Llorens F, Roher N, Plana M, Gómez N, Itarte E. Arch Biochem Biophys. 2002 406(2):165-72. 4. PP1/PP2A phosphatases inhibitors okadaic acid and calyculin A block ERK5 activation by growth factors and oxidative stress. Garcia L, Garcia F, Llorens F, Unzeta M, Itarte E, Gómez N. FEBS Lett. 2002 523(1-3):90-4. 3. Apigenin and LY294002 prolong EGF-stimulated ERK1/2 activation in PC12 cells but are unable to induce full differentiation. Llorens F, Garcia L, Itarte E, Gómez N. FEBS Lett. 2002 510(3):149-53. 2. The carboxy-terminal domain of Grp94 binds to protein kinase CK2 alpha but not to CK2 holoenzyme. Roher N, Sarno S, Miró F, Ruzzene M, Llorens F, Meggio F, Itarte E, Pinna LA, Plana M. FEBS Lett. 2001 505(1):42-6. 1. The C-terminal domain of human grp94 protects the catalytic subunit of protein kinase CK2 (CK2alpha) against thermal aggregation. Role of disulfide bonds. Roher N, Miró F, Boldyreff B, Llorens F, Plana M, Issinger OG, Itarte E. Eur J Biochem. 2001, 268:2, 429-437. (*) Equal contribution (#) Corresponding author INTERNATIONAL MEETINGS: Authors: Llorens F. Title: Functional Genomics on prnp models. Oral communication Meeting: 2nd Priority Meeting - FP7 – Seventh Framework Programme – UE Place: Zurich (Switzerland) Year: 2010. Authors: Llorens F, Iraola S, Gonzalez E, Mosquera AM, Ferrer A, Pastor X, Miñana B, Lozano J, Sumoy L. Title: Multi-platform study to assess expression profiles in HeLa cells in response to growth factor stimulation with correction for genome dosage effects. Panel communication Meeting: 10th Annual Microarray and Gene Expression Data Society (MGED) meeting Place: Brisbane (Australia) Year: 2007 Authors: Llorens F, Duarri A, Roher, N, Plana M, Itarte E. Title: CK2 phosphorylation sites on eIF2beta affect the rate of protein synthesis in HeLa cells. Panel communication Meeting: 12th International Conference on Second Messengers and Phosphoproteins. Place: Montreal (Canada) Year: 2004 Authors: Llorens F, Miró F, Roher N, García L, Plana M, Gómez N, Itarte, E. Title: Long term activation of ERK 1/2 by serum or apigenin in HeLa cells lead to different cell fate. Panel communication Meeting: Meeting of the Biochemical Society. Signalling the Future. Place: Liverpool (UK). Year: 2002 Authors: Llorens F, Roher N, Plana M, Itarte E. Title: CK2 interacts with the beta subunit of eukariotic inititaiton factor eiF2 Panel communication Meeting: 27º FEBS/PABMB Meeting. Place: Lisboa (Portugal) Year: 2001 JOURNAL REVIEWER: Journal Reviewer for: PLoS One INTERNATIONAL STAYS: Centre: Beatson Institute for Cancer Research. Glasgow (Scotland) Year: 2002 Length: 3 months Pre-Doctoral Stay Centre: Department of Biological Chemistry, University of Padova. Padova (Italy) Year: 2005 Length: 2 months Pre-Doctoral Stay Centre: Department of Biological Chemistry, University of Padova. Padova (Italy) Year: 2005 Length: 1 months Post-Doctoral Stay