16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-L-001 The science of chemical complex natural products: network pharmacology (Master Lecture) Spelman, Kevin Health, Education & Research in Botanical Medicine, Ashland, United States [email protected] drugable The The current model model in in drug drug discovery discovery isisbased basedon onthe theligand-receptor ligand-receptorparadigm paradigmasasthe the drugableunit. unit. The common analogy of drug action is that of a lock and key, with a drug acting as a selective 'key' that fits into the 'lock' of aa specific specific drug drug target. target. And Andwhile whilethis thisselectivity selectivityparadigm paradigmhas hasyielded yieldedefficacious efficaciousdrugs, drugs,many many drugs act actvia viamodulation modulationof of multiple multiple proteins: proteins: Many Many effective effective drugsdrugs in oncology, in oncology, psychiatry psychiatry and antiand antimicrobials act on multiple, rather rather than single single targets. targets. Systems Systems biology biology has has revealed revealed physiological physiological function function as a robust network structure that strongly suggests suggests that that exquisitely exquisitely selective selective compounds, compounds, compared compared with with multitargetdrugs, may may exhibit exhibitlower lowerthan than desired desired clinical clinical efficacy efficacy and induce and induce more adverse more adverse events. events . Chemically complex botanically-based medicines inherently act on multiple sites and therefore have never never fully been beenunderstood fully understood in a reductionist in a reductionist ligand-receptor ligand-receptor modelmodel well. However, well. However, botanical botanical medicines medicines fit intofita into a network network pharmacology pharmacology paradigm paradigm seamlessly. seamlessly. Viewing Viewing mixtures mixtures of natural of natural products products through through thethe lens lens of network how we wecan can improvedrug drug discovery utilize botanical of networkbiology biologymay mayprovide provide insights insights into how improve discovery and and utilize botanical medicines for complex diseases. Key words: science, chemical complex, natural products, pharmacology 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-L-002 Synthesis and evaluation of exo- methylene butyrolactone natural products- can they be made into drugs (Master Lecture) Reiser, Oliver Institut für Organische Chemie, Universität Regensburg, Germany oliver.reiser@chemie. uni-regensburg.de Exo-methylene-ƒÁ-butyrolactone natural products are abundant in nature that display broad biological profiles, among them high Exo-methylene-ƒÁ-butyrolactone natural tumorproducts activity. Despite are abundant their generally in nature high that display broad biological cytotoxicity, profiles, among these them compounds high tumor are activity. considered Despite to betheir unsuitable generally for drug high development, cytotoxicity, these compounds are owing their high considered to bebut unsuitable unspecific forreactivity drug development, with biological owing nucleophiles. their high but unspecific reactivity with biological We started a program nucleophiles. We started that a program combinesthat thecombines synthesisthe of exo-methylene-ƒÁ-butyrolactone synthesis of exo-methylene-ƒÁ-butyrolactone natural products focusingfocusing on asymmetric catalysis,[1] synthesis of peptide foldamers containing ƒÀ-amino acids[2] natural products on asymmetric catalysis,[1] synthesis of peptide foldamers as carriers ƒÀ-amino and evaluation of as hybrids derived thereof to of selectively recognize specific containing acids[2] carriers and evaluation hybrids derived thereof to cancer cells. Thus, following approachspecific we arecancer confident thatThus, a large body ofour biologically potent, but greatly neglected selectivelyour recognize cells. following approachhighly we are natural products and analogs might become promising for drug development in medicinal chemistry. confident that a large body of biologically highly potent, but greatly neglected natural products and analogs might become promising for drug development in medicinal chemistry. [1] a) A. Bergmann, O. Reiser, Chem. Eur. J. 2014, 20, 7613; b) A. Kreuzer, S. Kerres, T. Ertl, Key words: H. Rucker, synthesis, S. Amslinger, evaluation, O. Reiser, exo- methylene Org. Lett.butyrolactone, 2013, 15, 3420. natural product, drug [2] a) C. Cabrele, T. A. Martinek, O. Reiser, L. Berlicki , J. Med. Chem. 2014, 57, 9718. b) .. Berlicki, M. Kaske, R. Gutierrez-Abad, G. Bernhardt, O. Illa, R. M. Ortuno, C. Cabrele , A. Buschauer, O. Reiser, J. Med. Chem. 2013, 56, 8422; c) L. Berlicki, L. Pilsl, E. Weber, I. M. Mandity, C. Cabrele, T. A. Martinke, F. Fulop, O. Reiser, Angew. Chem. 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-L-004 Medicinal plants of the brazilian Caatinga biome: from popular use to scientific studies (Master Lecture) Guedes da Silva Almeida, Jackson Roberto Universidade Federal do Vale do São Francisco, Brazil jackson.guedes@univas f.edu. br The Caatinga Caatinga biome biome(semi-arid (semi-arid vegetation) vegetation) is a is highly a highly threatened threatened biome covering biome covering a vast area a vast in area in Northeastern Brazil and is the source of few studied natural resources. Many medicinal plant species from from Caatinga Caatinga are widely are widely knownknown and used and in used folk in medicine folk medicine and forand commercial for commercial manufacturing manufacturing of phytotherapeut of ic phytotherapeutic products. Few ethnobotanical products. Few and ethnobotanical pharmacological and studies pharmacological have beenstudies undertaken have in been thisundertaken region, in spite in of this great the region, cultural in spite and of biological the great cultural diversityand to be biological found there. diversity Thetopurpose be found of there. this lecture The purpose is to present of thisresults lecture of research is to present carried results out at of theresearch Federal carried University out atofthe Vale Federal do São University Francisco of Vale withdothe São species Francisco Annona with the species vepretorum, atemoia Annona (A. vepretorum, cherimola Mill. atemoia x A. (A. squamosa cherimola L.), Mill. Selaginella x A. squamosa convoluta, L.), Selaginella Hymenaeamartiana, convoluta, Hymenaeamartiana, Bromelialaciniosa, Encholiriumspectabile Bromelialaciniosa, and Neoglazioviavariegata, Encholiriumspectabile typical and Neoglazioviavariegata, species from the Caatinga typical species biome. The fundamentals from the Caatinga of thebiome. main The methods fundamentals for assessing of the main the antinociceptive, methods for assessing anti-inflammatory the , antinociceptive, anti-inflammatory, antiulcerogenic and central nervous antiulcerogenic system activity and central of a natural nervoussubstance system activity or plant of aextracts natural will be presented.The mainextracts chemical isolatedmain fromchemical extracts of these species will be presented substance or plant willconstituents be presented.The constituents isolated from extracts as of well as thespecies chemical of some oils. Regarding the pharmacological activity, results willthe be these willcomposition be presented as wellessential as the chemical composition of some essential oils. Regarding presented on theactivity, antinociceptive, antiulcerogenic andanti-inflammatory, on the central nervous system. pharmacological results willanti-inflammatory, be presented on the antinociceptive, Furthermore, in vitrophotoprotective and system. antioxidant activity wasthe also exact antiulcerogenictheand on the central nervous Furthermore, in investigated.The vitrophotoprotective andmechanism involved in the antinociceptive and anti- inflammatory activities isinvolved not completely understood but, at antileast in antioxidant activity was also investigated.The exact mechanism in the antinociceptive and inflammatory part there is the activities participation is not of completely opioid receptors understood and but, inhibition at least of cyclooxygenase in part there is the enzyme.Pharmacologic participation of opioid al receptors and studies inhibition cyclooxygenase and chemical studies continuing and chemical areofcontinuing in orderenzyme.Pharmacological to characterize the mechanism responsible forare these effects. Key words: medicinal plants, phytochemistry, pharmacology, natural products, caatinga 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-O-001 Therapeutic effects of policosanol and atorvastatin against global brain ischemiareperfusion injury in gerbils Molina Cuevas, Vivian; Ravelo Calzado, Yazmin; Noa Puig, Miriam; Mas Ferreiro, Rosa María; Pérez Guerra, Yohani; Oyarzábal Year, Ambar; Mendoza Hernández, Nilda; Jiménez Despaigne, Sonia; Sánchez López, Javier Vicente Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] El ictus isquémico constituye la tercera causa de muerte y la primera de discapacidad en la población adulta. El uso de agentes antiplaquetarios en el tratamiento del ictus isquémico ha reducido su recurrencia solo de forma moderada y se asocia a frecuentes eventos adversos por lo que la búsqueda de nuevas estrategias terapéuticas seguras y eficaces constituye una problemática actual. El policosanol, el extracto de semilla de uva y la atorvastatina son sustancias con reportados efectos antiplaquetarios. Este estudio compara los efectos preventivos del policosanol y el extracto de semilla de uva así como los efectos terapéuticos del policosanol con la atorvastatina en un modelo de isquemia cerebral en Mongolian gerbils. Los resultados mostraron que policosanol y extracto de semilla de uva presentaron eficacias similares para prevenir la isquemia cerebral mientras que el policosanol y la atoravastatina resultaron similarmente efectivas para tratar la isquemia cerebral previamente inducida en Mongolian gerbils. Key words: policosanol, atorvastatin, ischemia-reperfusion 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-O-002 Comparison of policosanol and grape seed extract on global cerebral isquemia in Mongolian gerbils Molina Cuevas, Vivian; Ravelo Calzado, Yazmin; Mena Valdés, Licet; Noa Puig, Miriam; Pérez Guerra, Yohani; Oyarzábal Year, Ambar; Mas Ferreiro, Rosa María; Valle Clara, Maikel; Mendoza Hernández, Nilda; Jiménez Despaigne, Sonia Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] El ictus isquémico constituye la tercera causa de muerte y la primera de discapacidad en la población adulta. El uso de agentes antiplaquetarios en el tratamiento del ictus isquémico ha reducido su recurrencia solo de forma moderada y se asocia a frecuentes eventos adversos por lo que la búsqueda de nuevas estrategias terapéuticas seguras y eficaces constituye una problemática actual. El ´policosanol, el extracto de semilla de uva y la atorvastatina son sustancias con reportados efectos antiplaquetarios. Este estudio compara los efectos preventivos del policosanol y el extracto de semilla de uva así como los efectos terapéuticos del policosanol con la atorvastatina en un modelo de isquemia cerebral en Mongolian gerbils. Los resultados mostraron que policosanol y extracto de semilla de uva presentaron eficacias similares para prevenir la isquemia cerebral mientras que el policosanol y la atoravastatina resultaron similarmente efectivas para tratar la isquemia cerebral previamente inducida en Mongolian gerbils. Key words: policosanol, grape seed extract, ischemia-reperfusion 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-O-005 Extraction of phenolic compounds with antioxidant activity of Champa (Campomanesia lineatifolia) Otálvaro Álvarez, Ángela María; Chaparro González, María Patricia; Pabon Baquero, Ludy Cristina; Rendón Fernández, Margarita Rosa; Muñoz Cadena, William Armando; Chávez Riaño, William Patricio Universidad de La Salle, Colombia [email protected] Champa (Campumanesialineatifolia), a fruit with high potential to be used as a treatment of chronical diseases due to its chemical composition, grown the municipality Miraflores (Boyaca, Colombia). Champa (Campumanesialineatifolia), a fruit iswith high inpotential to be usedof as a treatment of chronical Nowadays, post-harvest losses reach 97%,isbeing an opportunity value and to giveColombia). sustainability diseases due to its chemical composition, grownthis in the municipalitytoofadd Miraflores (Boyaca, to this crop.post-harvest The aim of this work was97%, to extract Champa's phenolic tocompounds andtotogive assess its antiNowadays, losses reach being this an opportunity add value and oxidant capacity. Lyophilized subjected to to a extract solid-liquid extraction (1:10),compounds with water,and ethanol sustainability to this crop. The fruit aim was of this work was Champa's phenolic to and ethanol-water mixtures capacity. (3:7) as solvents at three temperature 20, 50 y 70°C. Phenolic content assess its anti-oxidant Lyophilized fruit was subjectedlevels, to a solid-liquid extraction (1:10), with and anti-oxidant activity were determined for the(3:7) dry as extracts. Extraction ranged levels, from 26,85% 50,07%, water, ethanol and ethanol-water mixtures solvents at three yields temperature 20, 50 yto70°C. were the content higher numbers were obtained with water as a solvent. At extracts. tested conditions, Phenolic and anti-oxidant activity were determined for the dry Extractionwater yieldsshowed ranged no significant dependency extraction temperature. Phenolic content increased extraction temperature, from 26,85% to 50,07%,on were the higher numbers were obtained with water aswith a solvent. At tested in the rangewater of 1469,32 ± 152,14 to 5272,51 ± 424,89 on µg extraction of galic acid/g of lyophilized pulp, content where the higher conditions, showed no significant dependency temperature. Phenolic figures were obtained withtemperature, ethanol-water mix range (7:3). of Antioxidant activity to were found ±between 20,44 ± 2,57 increased with extraction in the 1469,32 ± 152,14 5272,51 424,89 µg of galic to 51,01 1,93 mM pulp, TEAC/g lyophilized pulp, beingwere the water andwith the ethanol-water mix (7:3). (7:3) the acid/g of ±lyophilized where the higher figures obtained Anti-solvents with higher values. significant effect on activity was observed temperature, different oxidant activity wereAfound between 20,44 ± 2,57 to 51,01 ± 1,93 with mM extraction TEAC/g lyophilized pulp,with being the trends depending on the mix solvent used. As with a result of this A research, It is on concluded water and the ethanol-water (7:3) the solvents higher values. significant effect activity wasthat Campumanesialineatifolia is a natural with product with trends a high depending content of on bio-active compounds, a great observed with extraction temperature, different the solvent used. As awith result of potential to be Itused in agricultural, pharmaceutical and cosmetic industries. this research, is concluded that Campumanesialineatifolia is a natural product with a high content of bio-active compounds, with a great potential to be used in agricultural, pharmaceutical and cosmetic Key words: campumanesialineatifolia, dpph, antioxidants, natural products 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-O-006 Hypoglycemic and Hypolipidemic effects of aqueous extract of "Estrella Dorada" in Experimentally Induced Diabetic Rats Álvarez Contreras, Carolina; Martínez Jorquera, Rolando Universidad Austral de Chile, Chile [email protected] The present study investigates the hypoglycemic and hypolipidemic effect of oral administration of queousextract of "Estrella dorada" a endemic bush corresponding to of the Astaraceae that Thepresentstudyinvestigatesthehypoglycemicand hypolipidemiceffect of the oral family administration of grows in the South of Chile.dorada" In this a study 36 Malehypolipidemiceffect Sprague-Dawley rats, body of 250-300 gr were aqueousextract of "Estrella endemicbushcorrespondingtothefamily of weight theAstaraceaethatgrows Thepresentstudyinvestigatesthehypoglycemicand of oral administration of divided into 4ofgroups. Diabetes wasa induced by three intra peritoneal injection of 75 mg/kg Alloxan. Four in the South Chile. aqueousextract of "Estrella dorada" endemicbushcorrespondingtothefamily of theAstaraceaethatgrows weeks after the first injection, the rats were fasted and blood glucose levels were recorded. Animals were In thisstudy 36 MaleSprague-Dawleyrats, bodyweight of 250-300 gr weredividedinto 4 groups. Diabetes in the South of Chile. considered diabeticperitoneal when plasma glucose higher than 200 mg/dl. Thee ffect of wasinducedbythreeintra injection of 75concentrations mg/kg Alloxan.were Fourweeksafterthefirstinjection, In thisstudy to 36 be MaleSprague-Dawleyrats, bodyweight of 250-300 gr weredividedinto 4 groups. Diabetes the extract on the and fast bloodglucoselevelswererecorded. ingserum triglycerides levels, Lipidbeperoxides (TBAS) in theratswerefasted Animalswereconsideredto diabeticwhen wasinducedbythreeintra peritonealglucose, injectiontotal of 75colesterol, mg/kg Alloxan. Fourweeksafterthefirstinjection, plasma and in other biochemical parameters after a ten week are expressed glucoseconcentrationswerehighert han analyzed 200 mg/dl. Theeffect of treatment. Results theratswerefasted and bloodglucoselevelswererecorded. Animalswereconsideredto be diabeticwhen as meanglucoseconcentrationswerehighert ± SD. The data were analyzed using of Dunet <0.05). The (TBAS) infusion insupply to theextractonthefastingserumglucose, totalhan colesterol, triglycerideslevels, Lipidperoxides plasma 200multipleTest mg/dl. Theeffect of (P diabetic rats decreased the blood glucose level(284.6±31.2 to 170.32±6.3 mg/dl) and values similar plasmaand in otherbiochemicalparametersanalyzedafter a ten weektreatment. Results are expressed theextractonthefastingserumglucose, total colesterol, triglycerideslevels, Lipidperoxides (TBAS) in toasthe observed inin control ratswereanalyzedusingmultipleTest were found (166.7±22.7 mg/dl).ofTreatment mean ± SD. The data Dunet (P showed <0.05). significantly plasmaand otherbiochemicalparametersanalyzedafter a ten weektreatment. Results hypolipidemic are expressedeffect as and in ±other parameters analyzed (LDL, HDL y TGA). the fasting plasma cholestero Theinfusionsupplytodiabeticratsdecreasedthebloodglucoselevel(284.6±31. 170.32±6.3 mg/dl) and mean SD. biochemical The data wereanalyzedusingmultipleTest of Dunet (P Results <0.05). 2onto lwere with untreated and treatment normal (166.7±22.7 rats (142.9 ±mg/dl). 3.2 and 108.3 ± 1.7 mg/dl), well as Theinfusionsupplytodiabeticratsdecreasedthebloodglucoselevel(284.6±31. values compared similar totheobserved in controlratswerefound 2 to 170.32±6.3 mg/dl)asand indiabetic control and diabeticintreated rats(140.6 ±and 12.4(166.7±22.7 111.0 ±mg/dl). 1.7 mg/dl). The data demonstrated that values Treatmentshowedsignificantlyhypolipidemiceffect similar totheobserved controlratswerefound inand otherbiochemicalparametersanalyzed (LDL, HDL the aqueous extract of "Estrelladorada" was associated with antidiabetic and hypolipidemic effects on the Treatmentshowedsignificantlyhypolipidemiceffect y TGA). Resultsonthefasting plasma cholesterolwerecomparedwithuntreatedand and in otherbiochemicalparametersanalyzed treatmentnormal (LDL, rats HDL yexperimentally (142.9 TGA).±Resultsonthefasting 3.2 andinduced 108.3 diabetic ± 1.7plasma mg/dl), rats.cholesterolwerecomparedwithuntreatedand as well as indiabetic control and diabetictreatedrats(140.6 treatmentnormal ± 12.4 rats and 111.0 ±± 1.7 (142.9 3.2 and 108.3 ± 1.7 mg/dl), as well as indiabetic control and diabetictreatedrats(140.6 ± 12.4 and 111 0 1 7 /dl) Key words: hypoglycemic, hypolipidemic, diabetic rats 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-O-007 Preliminary phytochemical analysis, antioxidant activity and inhibiting the angiotensin-converting enzyme (ACE) of the ethanol extract of the leaves of Passiflora quadrangularis L. Bareño, Lesly L.; Guerra C., Carlos M.; Guerrero, Mario F.; Jiménez, Ángel A. Universidad Nacional de Colombia, Colombia [email protected] Oxidative stress and and hypertension hypertension are are considered considered factors factorsprecursors precursors ofofcardiovascular cardiovasculardiseases diseasesaccording according to WHO are to the WHO leading are the cause leading of death causeworldwide, of death worldwide, treatment treatment exposes the exposes patientthe to patient the continued to the use of continued drugs thatuse leadof to drugs sidethat effects, lead totherefore side effects, it is therefore required itnew is required therapeutic new therapeutic sources that sources decrease. that The decrease. The Passiflora genus Passiflora comprises genus about comprises 500 species aboutthat 500have species widethat use have in folk widemedicine use in folk in medicine many countries; in many countries; including the species including Passiflora the species quadrangularis PassifloraLinn, quadrangularis properties are Linn, attributed properties to headache, are attributed hypertension, to headache, and diabetes hypertension, (Dhawan, K., andDhawan, diabetes S (Dhawan, & Sharma, K., Dhawan, 2004). According S & Sharma, to the 2004). above According in this to study the ACE above in this inhibitory activity study in ACE vitro by inhibitory the method activity of Simonetta in vitro by the Ronca method Testoni of Simonetta (Guerra, C; Ronca Peñalosa, Testoni G; (Guerra, Mendez, C; J& Peñalosa, Murillo, W.G; 2014), Mendez, antioxidant J & Murillo, activity W.by2014), ABTSantioxidant and preliminary activity phytochemical by ABTS andanalysis preliminary (AFP) phytochemical in the ethanol analysisof(AFP) extract the leaves in the of ethanol P. quadrangularis extract of thewas leaves evaluated, of P. quadrangularis which will establish was evaluated, preform which if it haswill effects on establish preform hypertension. Regarding if it has the effects ACEoninhibitory hypertension. activityRegarding was established the ACE that inhibitory the extract activity at awas concentration establishedof that the 0.05 mgextract / ml inhibited at a concentration 70%, whereas of of 0.05 0.05 the pattern mg mg //ml ml(Captopril) inhibited inhibited 70%, 70%, to 1.0whereas whereas mg / mlthe obtained thepattern pattern only (Captopril) (Captopril) 15%; antioxidant toto1.0 1.0mg mg activity with ABTS extract behaved activity similarlywith to the reference with the PPA also possible to ml only antioxidant similarly to the thewas reference standard, mlobtained obtained only 15%; 15%; antioxidant activity ABTS extractstandard, behaved similarly to reference standard, establish the presence of saponins, flavonoids alkaloids, of which flavonoids its effect onand hypertension is which known with the PPA was also possible to establish theand presence of saponins, alkaloids, of (Rojas, 2009). According to the results it follows that the extract has hypotensive effect, which must be confirmed with in vivo studies. Key words: passiflora quadrangularis l, antioxidant activity, hypertension, in vitro, preliminary 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-O-009 Cymbopogon citratus aqueous fraction do not protect against UVC damage in Caulobacter crescentus León Fuentes, Fabiana; García Fernández, F.; González Pumariega, M.; Sánchez Lamar, Ángel Universidad de La Habana (UH), Cuba [email protected] Ozone layer hole entails ultraviolet radiation increase on the earth surface, raising the risk of skin diseases, even Theentails use ofultraviolet vegetal compounds is a new strategy protectraising peoplethe against Ozonecancer. layer hole radiation increase on the earth to surface, risk of UV skindamages . Substances obtained natural sources have a huge molecular and biological diseases, even cancer.from The use of vegetal compounds is a new strategydiversity to protect people againstfunctions. UV However, evaluationobtained of the toxic these compounds required before its beneficial properties damages. the Substances fromcapacity natural of sources have a hugeismolecular diversity and biological can be used. Cymbopogon citratus (DC)toxic Stapf is consumed as a popularis decoction and has functions. However, the evaluation of the capacity of these compounds required before its several properties such as: anticancer, antitumor and antimutagenic. of consumed these studies assayed in total beneficial properties can be used. Cymbopogon citratus (DC) Many Stapf is as awere popular decoction extract, a less portionsuch was as: assayed in fractioned extract. In this work, we tested the toxicity the Many of these studiesand were and has while several properties anticancer, antitumor and antimutagenic. ability to protect against while UVC light of portion the aqueous fractionsinobtained Cymbopogon citratus. To dothe so, assayed in total extract, a less was assayed fractionedfrom extract. In this work, we tested SOS Chromotest, RifRtoand Survival testUVC werelight used in Caulobacter crescentus. Increasing concentrations toxicity and the ability protect against of the aqueous fractions obtained from Cymbopogon of these To fractions (0.1, 0.5, 1, 2 andRifR 4 mg/mL) were applied continuously: before, during and after UVC citratus. do so, SOS Chromotest, and Survival test were used in Caulobacter crescentus. exposure J/m2). Results showed that this fraction not4cytotoxic and don´t possess any genotoxic Increasing(45 concentrations of these fractions (0.1, 0.5, 1,was 2 and mg/mL) were applied continuously: properties. Aqueous fraction decrease the induction of SOS phenomenon, survival and frequency before, during and after UVC can´t exposure (45 J/m2). Results showed that this fraction was not cytotoxic andof mutations in irradiated cells. Finally, the aqueous Cymbopogon (DC) Stapf, do not protect don´t possess any genotoxic properties. Aqueousfraction fractionofcan´t decreasecitratus the induction of SOS phenomenon, DNA from primarily survival damage and frequency induced by of mutations UVC light. in irradiated cells. Finally, the aqueous fraction of Key words: cymbopogon citratus, aqueous fractions, sos chromotest, survival test, rifr test, caulobacter 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-O-010 Comparation of antioxidants properties and polyphenols content of aquoeus extract from seaweeds Bryothamniontriquetrum and Halimedaopuntia Díaz Gutiérrez, Daylín; Méndez Ortega, Wendy; de Oliveira e Silva, Ana Mara; Zaldívar Muñoz, Claudina; Mancini Filho, Jorge; Vidal Novoa, Alexis Universidad de La Habana (UH), Cuba [email protected] The current investigation focuses attention on the antioxidant properties of aqueous extracts of marine algae Bryothamnion triquetrum Halimeda opuntia, and the relationship of suchextracts properties with total The current investigation focusesand attention on the antioxidant properties of aqueous of marine polyphenol content, using different experimental in vitro antioxidant assessment models. We observed algae Bryothamnion triquetrum and Halimeda opuntia, and the relationship of such properties with total following Reducing power experimental (concentration 128 antioxidant mg/mL, B. assessment triquetrum OD=2.798 and H. opuntia polyphenolresults: content, using different in vitro models. We observed OD=0.800, ascorbic acid with 1 mg/mL OD=2,824), assays(B. triquetrum IC50=1.15 0.06 and H. following results: Reducing power (concentration 128DPPH mg/mL, B. triquetrum OD=2.798 and H.± opuntia opuntia IC50=12.34 0.30 mg/mL), inhibition of lipidDPPH peroxidation in rat brain homogenates triquetrum OD=0.800, ascorbic ±acid with 1 mg/mL OD=2,824), assays(B. triquetrum IC50=1.15 ±(B. 0.06 and H. CI50=5.09± 025 and H. opuntiaIC50=1.25± 031 mg/mL) and the inhibition of red blood cell (RBC) opuntia IC50=12.34 ± 0.30 mg/mL), inhibition of lipid peroxidation in rat brain homogenates (B. triquetrum haemolysis induced by AAPH (with 5 mg/mL B. triquetrum inhibited until 100 % and H. opuntia 24%). The CI50=5.09± 025 and H. opuntiaIC50=1.25± 031 mg/mL) and the inhibition of red blood cell (RBC) contents of induced polyphenolic compounds was forB.B.triquetrum triquetruminhibited 51,21 ±until 2,25100 and% H. opuntia 19,9924%). ± 1,12 µg haemolysis by AAPH (with 5 mg/mL and H. opuntia The GAE/ mgof extract. It was demonstrated thatforB.triquetrum extract more effective than 19,99 H. opuntia free contents polyphenolic compounds was B. triquetrum 51,21was ± 2,25 and H. opuntia ± 1,12inµg radical scavenge reducing power while H.opuntia was more effective in inhibition peroxidation GAE/ mg extract. Itand was demonstrated that B.triquetrum extract was more effective thanofH.lipid opuntia in free in rat brain homogenatesand inhibition of red blood (RBC) haemolysis induced AAPH.Both radical scavenge and reducing the power while H.opuntia was cell more effective in inhibition of lipidbyperoxidation extractswerenottoxicin the model ofacute oftoxicity ofArtemiasalina. These resultssupporting itspossible in rat brain homogenatesand red blood cell (RBC) haemolysis induced by AAPH.Both the inhibition application asphytodrugand /ornutraceuticalfor its antioxidant propertiesand low toxicity. extractswerenottoxicin the model ofacute ossible Key words: seaweed, natural antioxidants, halimeda opuntia, bryothamnion triquetrum, polyphenol 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-O-011 Structural identification of obtained compounds from the ethanolic extract of Dioscorea bartlettii Morto Ceja Ochoa, Irais Araceli; García Vicencio, Miguel; Martínez Rocha, Alejandra; Sánchez López, Abigail Cristal; Berdeja Martínez, Blanca Margarita; Fregoso Aguilar, Tomás Alejandro Instituto Politécnico Nacional, México [email protected] Los productos productos de de origen origen vegetal vegetalson sonrecursos recursosrenovables renovablesdede múltiples usos el hombre, múltiples usos parapara el hombre, entreentre los los cuales se se encuentran encuentran el el uso usomedicinal medicinaly yeleltoxicológico. toxicológico. información obtenida la investigación LaLa información obtenida de de la investigación de de compuestos de origen origen vegetal vegetal ayuda ayuda aacomprender comprender lalafisiología fisiologíay ybioquímica bioquímicadedeloslosorganismos organismosque queloslos producen y poder lograr lograr su su mejor mejor aprovechamient aprovechamientoo desde desde elelpunto punto de devista vistacientífico, científico,médico médicoy yeconómico. Dentro de la composición química de los productos vegetales se pueden considerar dos grupos: Productos económico. del metabolismo primarioquímica y Productos del metabolismo secundario. que destacar que muchos Dentro de la composición de los productos vegetales se pueden Hay considerar dos grupos: metabolitos respuestas fisiológicas secundario. muy específicas, en muchos casos Productos delsecundarios metabolismodesencadenan primario y Productos del metabolismo uniéndose a determinados receptores con secundarios una destacable complementariedad. hecho proporciona Hay que destacar que muchos metabolitos desencadenan respuestasEste fisiológicas muy un importante y justificación para el estudio de nuevos productos de origen animal o específicas, enincentivo muchos casos uniéndose a determinados receptores con unanaturales destacable vegetal, como lo son Este los compuestos que se puedan encontrar en la planta de estudio: complementariedad. hecho proporciona un importante incentivo y justificación paraDioscorea el estudiobartlettii de Morton. El objetivo naturales del trabajodeesorigen separar y purificar, en la medida quelos sea posible, losque compuestos nuevos productos animal o vegetal, como lo son compuestos se puedanque se encuentran hojasdede Dioscorea bartlettii MortonMorton. para posteriormente tratar de elucidar su estructura encontrar enenlalas planta estudio: Dioscorea bartlettii mediante de infrarrojo y de resonancia magnética elucidación de El objetivoespectroscopia del trabajo es separar y purificar, en la medida que sea nuclear. posible, La los separación compuestosy que se dichos compuestos nos llevó a la conclusión de Morton que unopara de los componentes tratar que más fácilmente encuentran en las hojas de Dioscorea bartlettii posteriormente de elucidar su se separó de dicha planta fue espectroscopia una saponina. La contiene también otros metabolitos estructura mediante de planta infrarrojo y de resonancia magnética nuclear.pero no se separaron La separación y elucidación de dichos compuestos nos llevó a la conclusión de que uno de los fácilmente. Key words: dioscorea, separación, metabolitos secundarios, saponina 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-O-012 Influence of operating conditions on the extraction of polyphenols from Moringa oleifera Lam leaves Valdés Hernández, Geidy Vivian; Comet Rodríguez, Raidel; Cruz Viera, Luis Instituto Superior Politécnico José Antonio Echeverría (Cujae), Cuba [email protected] Polyphenols are secondary metabolites present in plants, which have gained popularity for its wide antioxidant Numerous investigations have shown that the gained leaves popularity of Moringaforoleifera Polyphenolsproperties. are secondary metabolites present in plants, which have its wideLam are rich in this type of substance. The investigations aim of the present is that to study the influence of operating conditions antioxidant properties. Numerous have work shown the leaves of Moringa oleifera Lam are on extraction polyphenols solid-liquid contactwork fromisthe of this plant. The experimental richthe in this type ofofsubstance. Thebyaim of the present to dried study leaves the influence of operating conditions design consideredofthe type of solvent (ethanoliccontact solutions different concentrations), temperature (50, 60 on the extraction polyphenols by solid-liquid fromatthe dried leaves of this plant. The and 70°C) and the considered solute-solvent and(ethanolic 1:35 m/v) ratio. The extraction kinetics were also experimental design the (1:15, type of1:25 solvent solutions at different concentrations), evaluated. investigated temperatures, the activation energy the m/v) process was the solute-solvent (1:15, 1:25 andof1:35 ratio. Thedetermined. extraction From temperatureFrom (50, the 60 and 70°C) and the above conditions, those that reported the best results were ethanol solutionenergy to 60% as solvent, the investigated temperatures, the activation of (v/v) the process kinetics were also evaluated. From solute-solvent ratio 1:35 with an extraction 30 minutes. Forresults these were conditions, polyphenol ethanola solution to was determined. From the(m/v) above conditions, thosetime that of reported the best content 17,5 mg gallic acid equivalent/g dry(m/v) masswith withanantioxidant mg Trolox equivalent / g 60% (v/v)of as solvent, solute-solvent ratio 1:35 extraction activity time of of 30 35 minutes. For these dry mass, was obtained. The temperature effect significant in investigated range. The nature of conditions, a polyphenol content of 17,5 mg gallicwas acidnot equivalent/g drythe mass with antioxidant activity of 35 mg leaching the Trolox equivalent/g is physicaldry considering that the activation energy was effect 11,47was ± 0,96 mass, was obtained. The temperature not kJ/mol. significant in the investigated range. The nature of the leaching is physical considering that the activation energy was Key words: moringa, total polyphenols content, extraction kinetics, antioxidant activity 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-O-013 In vitro evaluation of three biological agents against ticks Otobius megnini y Rhipicephalus (Boophilus) microplus in the state of Mexico Gayón Amaro, Susana Gabriela; Beltrán León, Trinidad; Castelán Ortega, Octavio A.; Velasco Galindo, Edelmira México [email protected] Biological control is a tactic to regulate plague populations through the use of natural elements. Tick infestationcontrol affects isany animal important economical In México, Biological a tactic to species regulate with plague populations throughand thehealth use ofrepercussions; natural elements. Tick losts about 48 millions dollars per year had estimated becauseand of this parasitosis in cattle;Inthat is why the infestation affects of any animal species withbeen important economical health repercussions; Mexico, main purpose of this research was to evaluate the in-vitro effect of two organics and a microbial substances losts about 48 millions of dollars per year had been estimated because of this parasitosis in cattle; that is in the of adults Otobius was megnini y Rhipicephalus 150organics adult ticks genres why theviability main purpose of ticks this research to evaluate the in-vitromicroplus. effect of two andofa each microbial were collected from bovines; through those 15 groups with 10 subjects were made to 150 prove three biological substances in the viability of adults ticks Otobius megnini y Rhipicephalus microplus. adult ticks of agents: essential of Juniperus communis, metanolic of Annona purified each genres were oil collected from bovines; through thoseextract 15 groups with 10muricata subjectsseeds, were made to spore prove of M. anisopliae andessential distillatedoilwater with tween 80 to 0.1% for control groups (3) in four different levels three biologicalMa14 agents: of Juniperus communis, metanolic extract of Annona muricata seeds, of concentration using the immersion method. The results 15 daily observations purified spore of M. anisopliae Ma14 and distillated water withwere tweenestablished 80 to 0.1% after for control groups (3) in through a stereoscopic microscopyusing getting maximum mortality ratings of 50% after and 100% four different levels of concentration the minimum immersionand method. The results were established 15 respectively. According those resultsmicroscopy it is possible to design integral plague management systems daily observations throughwith a stereoscopic getting minimum and maximum mortality ratings of which consider natural elements like thewith entomopathogenic Metarhizium anisopliae Ma14, soursop 50% and 100% respectively. According those results it isfungus possible to design integral plague seeds (Annona muricata) andconsider pinus (Juniperus communis) agents with ixodicide potential against adult management systems which natural elements like like the entomopathogenic fungus Metarhizium ticks of theMa14, genressoursop Rhipicephalus microplus y Otobius anisopliae seeds (Annona muricata) andmegnini. pinus (Juniperus communis) like agents with Key words: veterinary medicine, biological control, ectoparasites, ticks 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-O-014 Antiinflamatory effects of Abexol (D-002) Molina Cuevas, Vivian; Ravelo Calzado, Yazmin; Mas Ferreiro, Rosa María; Zamora Rodríguez, Zullyt Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] La inflamación constituye un factor etiológico importante en el desarrollo de enfermedades crónicas como la osteoartritis, el cáncer, la hiperplasia prostática benigna, entre otras. Los anti-inflamatorios no esteroidales (AINEs) son medicamentos muy efectivos empleados para tratar la inflamación aguda, crónica y el dolor asociado; sin embargo su uso constituye una de las causas principales de úlcera gástrica. Por tal motivo la búsqueda de nuevas sustancias anti-inflamatorios con buen perfil de seguridad gastrointestinal constituye una problemática actual. Este trabajo evaluó los efectos del D-002, mezcla de seis alcoholes alifáticos de alto peso molecular obtenida de la cera de abejas, en modelos experimentales de inflamación y úlcera gástrica así como su posible gastrotoxicidad. La administración oral con D-002 en roedores redujo de forma significativa y moderada la inflamación en diferentes modelos experimentales, mientras que a diferencia de los AINES no produjo gastrotoxicidad y por el contrario protegió la mucosa gástrica. Key words: Abexol, antiinflamatory effect, drug, natural product 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-O-015 Gastrotoxic and gastroprotector effects of Abexol (D-002) Molina Cuevas, Vivian, Ravelo Calzado, Yazmin; Mas Ferreiro, Rosa María; Zamora Rodríguez, Zullyt Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] La inflamación constituye un factor etiológico importante en el desarrollo de enfermedades crónicas como la osteoartritis, el cáncer, la hiperplasia prostática benigna, entre otras. Los anti-inflamatorios no esteroidales (AINEs) son medicamentos muy efectivos empleados para tratar la inflamación aguda, crónica y el dolor asociado; sin embargo su uso constituye una de las causas principales de úlcera gástrica. Por tal motivo la búsqueda de nuevas sustancias anti-inflamatorios con buen perfil de seguridad gastrointestinal constituye una problemática actual. Este trabajo evaluó los efectos del D-002, mezcla de seis alcoholes alifáticos de alto peso molecular obtenida de la cera de abejas, en modelos experimentales de inflamación y úlcera gástrica así como su posible gastrotoxicidad. La administración oral con D-002 en roedores redujo de forma significativa y moderada la inflamación en diferentes modelos experimentales, mientras que a diferencia de los AINES no produjo gastrotoxicidad y por el contrario protegió la mucosa gástrica. Key words: Gastrotoxic, gastroprotector effects, Abexol D-002 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-O-016 Effects of Abexol (D -002) on esophagitis induced by gastroesophageal reflux in rats Zamora Rodríguez, Zullyt; Molina Cuevas, Vivian; Ravelo Calzado, Yazmin; Mas Ferreiro, Rosa María; Pérez Guerra, Yohani; Oyarzábal Year, Ambar; Jiménez Despaigne, Sonia Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] La enfermedad de reflujo gastroesofágico (ERGE), es una una patología patología crónica, donde donde alrededor alrededor del del 40 40 % % de la mundial la sufre. La ineficacia de los concon inhibidores de la de protones y la depoblación la población mundial la sufre. La ineficacia de tratamiento los tratamiento inhibidores debomba la bomba de protones aparición de reacciones adversas, hacen que que sea sea esteeste hecho una una problemática de salud en laenactualidad. y la aparición de reacciones adversas, hacen hecho problemática de salud la Con el objetivo evaluar de el efecto del D-002 del en D-002 diferentes modelos experimentales actualidad. Con de el objetivo evaluarprotector el efectoesofágico protector esofágico en diferentes modelos de esofagitis, sedeemplearon estudio ratas macho, a las seales aguda, experimentales esofagitis,en seelemplearon en elSD estudio ratas SDcuales macho, lasindujo, cualeslaseesofagitis les indujo, la en la cual aguda, se evaluó efecto citoprotector 5 hcitoprotector después de 5lahinducción; el la modelo de esofagitis esofagitis en laelcual se evaluó el efecto después de inducción; el modelogastrode duodeno-esofágico, el que fue evaluado 48fue h después induccióndey la el inducción modelo de reflujo esófagoesofagitis gastro-duodeno-esofágico, el que evaluado de 48 la h después y el modelo de duodenal con gastrectomía con una duración 14 días operatorio. Los resultados reflujo esófago-duodenal con gastrectomía con unamáxima duracióndemáxima de post 14 días post operatorio. Los demostraron el efecto protector del D-002 del en D-002 los modelos experimentales, con excepción resultados demostraron el efecto esofágico protector esofágico en los modelos experimentales, con del modelo de del reflujo-gastro-duodeno-esofágico. Dicho efecto protector estuvo mediadoestuvo por lamediado inhibiciónpor delala excepción modelo de reflujo-gastro-duodeno-esofágico. Dicho efecto protector peroxidación lipídica y proteico sin interferir en sin el volumen la acidez del ni jugo gástrico. inhibición de la peroxidación lipídica y proteico interferir de en secreción el volumennide secreción la acidez del Se concluye, que se efecto esófago protector D-002 protector en la esofagitis inducida reflujo gastrojugo gástrico. Sedemostró concluye,elque se demostró el efecto del esófago del D-002 en lapor esofagitis esofágicoy esófago-duodenal. Key words: Abexol, D -002, esophagitis, gastroesophageal reflux, rats 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-O-018 Effects of Abexol in patient with osteoarthritis Illnait Ferrer, José; Fernández Travieso, Julio César; Puente, Roberto; Mendoza Castaño, Sarahí; Mas Ferreiro, Rosa María; Carbajal D., Daisy; Mesa, Meilis; Gámez Menéndez, Rafael; Suárez, Pablo Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] Background and purpose: purpose: Osteoarthritis Osteoarthritis (OA) (OA)isisa adegenerative degenerativejoint jointdisease disease characterized joint pain characterized by by joint pain and dysfunction caused by by aa progressive progressive and and irreversible irreversible loss lossofofarticular articularcartilage, cartilage,that thataffects affectshundreds hundreds of millions millionsworldwide, worldwide,mainly mainly elderly. The management OA included a combination thethe elderly. The management of OA of included a combination of non- of nonpharmacological interventions and and pharmacologic pharmacologic agents. agents. Non Nonselective selectivenon-steroidal non-steroidalanti-inflammatory anti-inflammatory drugs inhibitors provide provide symptoms symptoms relief relief in in OA, OA, but but produce produce several several adverse adverse effects effects drugs and and ciclooxygenase ciclooxygenase 22 inhibitors (AE), which supports supports the the search search for for other other options. options. Abexol, Abexol, aamixture mixture of of higher higher aliphatic aliphatic beeswax beeswax alcohols, alcohols, (AE), which has effects experimentally experimentally and and clinically. clinically. This This double-blind double-blind study has been been shown shown to to produce produce anti-inflammatory anti-inflammatory effects investigated the effects of Abexol (50-100 mg/day) on patients with OA symptoms randomized to Abexol study investigated the effects of Abexol (50-100 mg/day) on patients with OA symptoms randomized to or placebo for 6 weeks. Methods: The primary efficacy outcome was the reduction of the total Western Ontario Abexol or placebo for 6 weeks. and McMaster Individual Osteoarthritis (WOMAC) efficacyand outcome was the Methods: The primary efficacy outcome Index was the reductionscore. of the The totalsecondary Western Ontario McMaster reduction pain, joint stiffness, physical activity scores, and the efficacy reductionoutcome of the VAS The collateral Individual on Osteoarthritis Index (WOMAC) score. The secondary wasscores. the reduction on efficacy outcome was reduction the use of rescue medications. tolerability assessed. pain, joint stiffness, physical activity scores, and the reduction ofTreatment the VAS scores. The was collateral efficacy Results: study completion Abexol (50-100 mg/day) significantly thewas total WOMAC score versus outcome At was reduction the use of rescue medications. Treatment reduced tolerability assessed. baseline (p<0.00001, 77.1%) and placebo (p <0.0001, 65.4%), and also decreased the pain (54.9%), joint Results: At study completion Abexol (50-100 mg/day) significantly reduced the total WOMAC score stiffness (76.8%)(p<0.00001, and physical77.1%) activityand (66.9%) scores versus placebo. Afteralso 1 week on therapy all benefits versus baseline placebo (p <0.0001, 65.4%), and decreased the pain were significant already. In addition, Abexol significantly reduced theplacebo. VAS score versus baseline (54.9%), joint stiffness (76.8%) and physical activity (66.9%) scores versus After 1 week on (p<0.00001, 60.2%)were and placebo (p already. <0.0001,In46.8%). use of rescue medication in Abexol (6/30, 20%) therapy all benefits significant addition,The Abexol significantly reduced the VAS score was lower (p <0.01) than in placebo 56.7%).(pTreatment was well The tolerated. (2 Abexol, versus baseline (p<0.00001, 60.2%)(17/30; and placebo <0.0001, 46.8%). use ofSeven rescuepatients medication in 5 placebo) reported moderate AE. Conclusions: Abexol (50-100 mg/day) given for 6 weeks improved Abexol (6/30, 20%) was lower (p <0.01) than in placebo (17/30; 56.7%). Treatment was well tolerated. OA symptoms and was well tolerated. Abexol could be a suitable Seven patients (2 Abexol, 5 placebo) reported moderate AE. option for managing OA symptoms, but this appreciation extensive further given research. Conclusions: requires Abexol (50-100 mg/day) for 6 weeks improved OA symptoms and was well tolerated. Abexol could be a suitable option for managing OA symptoms, but this appreciation requires extensive Key words: Abexol, osteoarthritis 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-O-019 Carcinogenicity of D-004 Gutiérrez Martínez, Ariadne; Gámez Menéndez, Rafael; Noa Puig, Miriam; Valle Clara, Maikel; Mendoza Hernández, Nilda; Pérez Guerra, Yohani; Oyarzábal Year, Ambar; Bucarano, I.; Goicochea, E.; Jiménez Despaigne, Sonia; García, H. Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] El D-004 es un un extracto extracto lipídico lipídico del del fruto fruto de delalaRoystonea Roystonearegia regia(palma (palmareal), real),constituido constituidopor poruna unamezcla mezcla de ácidos ácidos grasos, grasos,principalmente principalmenteoleico, oleico, palmítico, láurico y mirístico, quemostrado ha mostrado prevenir la palmítico, láurico y mirístico, que ha prevenir la hiperplasia prostática inducida con testosterona, no con dihidrotestosterona, dihidrotestosterona, en en roedores. roedores. El El objetivo objetivo del del presente estudio consistió en determinar si el tratamiento oral a largo plazo plazo (24 (24 meses) meses) con con D-004 D-004 (500(5001500 mg/kg) muestra evidencias evidencias de de toxicidad toxicidad y/o y/ocarcinogenicidad carcinogenicidad en enratas ratasSD SDadultas adultasjóvenes jóvenesdedeambos sexos. animales se distribuyeron aleatoriamente en cuatro (50/sexo/grupo), un grupo ambos Los sexos. Los animales se distribuyeron aleatoriamente en grupos cuatro grupos (50/sexo/grupo), uncontrol grupo al cual al sólo sesólo le administró el vehículo Tween Tween 65/H2065/H20 y tres ygrupos tratados con D-004 a las a dosis control cual se le administró el vehículo tres grupos tratados con D-004 las de 500, y 1500 tratamientos se administraron oralmenteoralmente por intubación gástrica una vez al dosis1000 de 500, 1000mg/kg. y 1500Los mg/kg. Los tratamientos se administraron por intubación gástrica día 24 durante meses. 24 La meses. mortalidad (análisis de(análisis supervivencia), tiempo de aparición tumores,designos una durante vez al día La mortalidad de supervivencia), tiempo dede aparición clínicos, de alimentos, hematológicos y de bioquímicay de sanguínea, tumores, peso signoscorporal, clínicos,consumo peso corporal, consumoparámetros de alimentos, parámetros hematológicos peso de órganos e incidencia de lesiones neoplásicas y no neoplásicas bioquímica sanguínea, peso de órganos e incidencia de lesiones neoplásicasdiferencias no neoplásicas ynonomostraron significativas ni tendencias con las dosis entre animales y controles ambos ysexos. Por tanto, mostraron diferencias significativas ni tendencias con lastratados dosis entre animalesentratados controles en el D-004sexos. no incrementa acelera la la aparición de lesiones o malignas, ambos Por tanto,laelfrecuencia D-004 no o incrementa frecuencia o aceleraneoplásicas la apariciónbenignas de lesiones ni induce la benignas aparición odemalignas, tumores ni inusuales la especie, muy similar la frecuencia de neoplásicas induce laaaparición de comportándose tumores inusuales a la especie, tumores entre los grupos tratados y controles. Los resultados estudio permiten concluir Los que el D-004 comportándose muy similar la frecuencia de tumores entre losdel grupos tratados y controles. no es tóxico carcinogénico en concluir ratas SDque tratadas durante meses y que la dosisen mayor mg/kg) resultados delo estudio permiten el D-004 no es24 tóxico o carcinogénico ratas (1500 SD tratadas se comportó como yelque nivella donde no se (1500 producen efectos tóxicos observables. durante 24 meses dosis mayor mg/kg) se comportó como el nivel donde no se producen Key words: Carcinogenicity, D-004 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-O-020 Effects of D-004 in patient with benign prostatic hyperplasia Guzmán, Raúl Hospital Salvador Allende ,, Cuba [email protected] Benign prostatic hyperplasia (BPH), common among elderly men, often causes lower urinary tract symptoms (LUTS). BPH/LUTS therapies 5- -reductase inhibitors, 1Benign prostatic hyperplasia (BPH),pharmacological common among elderly men,include often causes lower urinary tract adrenoreceptors blockers and phytotherapies, saw palmetto lipid extracts. D-004, a1-lipid extract of symptoms (LUTS). BPH/LUTS pharmacologicallike therapies include(SP) 5- -reductase inhibitors, Roystonea regia fruits, reduced experimental prostate in rodents and theD-004, International Prostate adrenoreceptors blockers and phytotherapies, like sawhyperplasia palmetto (SP) lipid extracts. a lipid extract Symptoms Score effectively as SP in aprostate pilot trialhyperplasia in men withinBPH. Thisand work two clinical of Roystonea regia(IPSS) fruits,as reduced experimental rodents theincludes International trials conducted in order compare the effects as of SP D-004 with BPH. In the phase II Prostate Symptoms Scoreto(IPSS) as effectively in aand pilotSP trialininpatients men with BPH. This workfirst includes study sixty-one withinmoderate BPH werethedouble-blindly randomised D-004 or SPBPH. (320 Inmg/day two clinical trialspatients conducted order to compare effects of D-004 and SP intopatients with the both) capsules for 4sixty-one months. patients Decrease onmoderate IPSS wasBPH the primary efficacy variable. Effects on prostate first phase II study with were double-blindly randomised to D-004 or size, SP residual volume post-voiding and4 serum prostate specific antigen were secondary outcomes. Afteron2 (320 mg/day both) capsules for months. Decrease on IPSS was(PSA) the primary efficacy variable. Effects months therapy, D-004 and post-voiding SP decreased IPSS valuesspecific versus antigen baseline(PSA) and this increased prostateon size, residual volume andmean serum prostate wereeffect secondary at study completion, whenonIPSS decreased 36.0% D-004 mean and 34.1% with SP, without differenc outcomes. After 2 months therapy, D-004 and SP with decreased IPSS values versus baseline and es this effectgroups. between increased Bothattreatments study completion, did not modify when IPSS prostate decreased size, residual 36.0%volume with D-004 post- and voiding. 34.1% Twowith SP-treated SP, patients withdrew from the study, oneBoth due to an adverse One D-0042 SP-treated patients without differences between groups. treatments didexperience. not modify prostate size,and residual volume postexperienced experiences. These results were confirmed a second phase III study. One voiding. Two adverse SP-treated patients withdrew from the study, one dueinto an adverse experience. One hundred D-004(100) patients withexperienced mild to moderate were double-blindly randomised to D-004 SP (320 and 2 eligible SP-treated patients adverseBPH experiences. These results were confirmed in aorsecond mg/day capsules for 24 weeks. After 2patients months with on therapy, D-004 andBPH SP lowered mean IPSS values . phase IIIboth) study. One hundred (100) eligible mild to moderate were double-blindly This effect increased the study, IPSSboth) decreasing 70.3% with D- 004After and 269.0 % with after 6D-004 months randomised to D-004 over or SP (320 mg/day capsules for 24 weeks. months on SP therapy, on groups. Both over treatments decreased significantly the with residual andtreatment, SP loweredwithout mean differences IPSS values.between This effect increased the study, IPSS decreasing 70.3% Dvolume as after compared to baseline. D-004, not SP, reducedbetween significantly, butBoth modestly, the 004 and post-voiding 69.0 % with SP 6 months on treatment, without differences groups. prostate size and PSA values versus baseline. No significant between group to differences treatments decreased significantly the residual volume post-voiding as compared baseline. were D-004,found. not Summarizing D-004 (320 but mg/day) was as SP (320 mg/day) decreasing LUTS in men with SP, reduced significantly, modestly, theeffective prostate as size and PSA values for versus baseline. No significant moderate BPH. Both treatments were well tolerated. betwe Key words: D-004, patient, benign prostatic hyperplasia 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-O-021 Phytochemical analysis and wogonin isolation from Scutellaria havanensis Jacq Marrero Delange, David; Morales Rico, Carmen Luisa; Salas Oliver, Eva; Cuéllar Cuéllar, Armando; González Canavacciolo, Víctor L. Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] Scutellaria havanensis Jacq. species, known as Escudo de la Habana (Havana skullcaps), is a Cuban endemic and it is Jacq. etnomedically treatment scabies,(Havana and as skullcaps), diaphoretic is and febrifuge. Scutellariaplant havanensis species, used knownforasthe Escudo de laofHabana a Cuban endemic It is also cultivated plant and it foris ornamental etnomedically purposes. used forSeveral the treatment pharmacologic of scabies, researches and as diaphoretic have confirmed and febrifuge. that total extracts or flavonoids of Scutellaria genusSeveral have pharmacologic shown antiinflammatory, antithrombotic, It is also cultivated for ornamental purposes. researches anxiolityc, have confirmed that total neuroprotective, anticonvulsivant, antitumor, antiarthritic, antiviral, and antimicrobial extracts or flavonoids of Scutellariaantioxidant, genus haveanticancer, shown antiinflammatory, anxiolityc, antithrombotic, properties. Little is known about the chemical composition of this plant. In this regards, the and aim of this work neuroprotective, anticonvulsivant, antioxidant, anticancer, antitumor, antiarthritic, antiviral, was to carry out a phytochemical study about of the the ethereal, methanolic and aqueous extracts from aerial the parts antimicrobial properties. Little is known chemical composition of this plant. In this regards, (leaves andwork stems) havanensis species. Saponins, bitter principles, groups, alkaloids, aim of this wasoftoS.carry out a phytochemical study ofsugars, the ethereal, methanolicamino and aqueous extracts flavonoids, coumarins, steroids, quinones andSaponins, resins, insugars, the extracts were qualitatively from aerial parts (leaves triterpenes and stems)and of S. havanensis species. bitter principles, amino identified. The wogonin flavone was crystallized fromsteroids, the ethereal extract, which inwas structurally groups, alkaloids, flavonoids, coumarins, triterpenes and quinones and resins, the extracts characterized by their GC-MS NMR spectra. The crystallized identified groups of compounds in thiswhich plant,was mainly were qualitatively identified. Theand wogonin flavone was from the ethereal extract, flavonoids, be of potential interest. structurally could characterized by theirpharmacological GC-MS and NMR spectra. The identified groups of compounds in this Key words: scutellaria havanensis, phytochemical, flavonoids, wogonin, lamiaceae, gc-ms 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-O-022 Natural Products from Palauan Traditional Medicine increase Insulin Levels and Reduce Blood Sugar Kitalong, Christopher U.; Figueroa, Mario; Kitalong, Ann; Balick, Michael J.; Graz, Bertrand; Yano, Victor Palau Republic [email protected] On the the islands islands ofofPalau, Palau,traditional traditionalplant plantmedicines medicines have been in the survival of small the small isolated have been vitalvital in the survival of the isolated populations for generations, pre and post-foreign contact. This traditional medicinal knowledge knowledge has mostly mostly been passed passed on onthrough throughoral oraltradition. tradition. Through ethnobotanical ethnomedical surveys, a Palauan Through ethnobotanical andand ethnomedical surveys, a Palauan panacea, that most widely used used for for the thetreatment treatment ofofdiabetes diabetesmellitus mellitustype typeIIIIwas wasanalyzed analyzedforforphytochemical content. Mangiferin, a compound in significant in this Palauan Traditional with phytochemical content. Mangiferin,found a compound found quantities in significant quantities in this Palauanmedicine Traditional diabetes activity, was measured in aqueous preparations traditional medicine and compared medicine inhibition with diabetes inhibition activity, was measured in aqueousofpreparations of traditional medicine to and compared those of organictosolvent those ofextracts. organic Aqueous solvent extracts. extractsAqueous were most extracts effective were in isolating most effective mangiferin. in isolating The insulin mangiferin. of stimulation The theinsulin extracted stimulation mangiferin of the was extracted measured mangiferin using a ?-cell was measured stimulationusing assay. a ?-cell Insulinstimulation secretion was assay. Insulin induced by the secretion extracted wasmangiferin induced byinthea extracted glucose mangiferin concentration in a dependent glucose concentration manner. Further dependent clinical manner. Further mechanistic trialsclinical were performed mechanistic to trials assess were blood performed sugar levels to assess and insulin blood quantities sugar levels in and human insulin serum with quantities and withoutin traditional human serum medicine with and preparations without traditional during anmedicine Oral Glucose preparations Tolerance during Test.anInsulin Oral Glucose secretion and blood sugarTest. levelsInsulin showed potential this Palauan traditional therapeutic for treatment of Tolerance secretion andofblood sugar levels showedmedicine potentialasofathis Palauan traditional type II Diabetes Mellitus. Key words: palau, mangiferin, diabetes mellitus type ii 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-O-023 The anti-inflammatory activity of Artemisia afra Pool, Edmund John; Kisten, Najwa University of the Western Cape, South Africa [email protected] Artemisia afra is a shrub indigenous to South Africa and has been used for medicinal purposes. This study investigated the effects of leaf extracts activity using usingRAW264.7 RAW264.7 study investigated the effects of leaf extractson on inflammatory/anti-inflammatory activity macrophages. Curcumin, Curcumin, aaknown knownanti-inflammatory anti-inflammatoryphytochemical phytochemical was also analysed concurrently was also analysed concurrently to to compare the relative effectiveness of these two phytoproducts. phytoproducts. Interleukin Interleukin 66 and and nitric nitric oxide oxide were were used used as as biomarkers for inflammation. Artemisia Artemisia was was extracted extracted with with ethanol. ethanol. RAW264.7 RAW264.7macrophages macrophages were werecultured cultured to confluence to confluence in 96 well in culture 96 well trays. culture Extracts trays. Extracts were tested were for tested anti-inflammatory for anti-inflammatory activityactivity by the by following the following Medium method: method: was Medium replaced was replaced with medium with medium containing containing various various concentrations concentrations of leave of leave extracts extracts in the in the presence presence and absence and absence of 1 µg/ml of 1 µg/ml LPS. LPS. After After 24 hour 24 incubation hour incubation culture culture supernatants supernatants were were removed and removed tested for and nitrictested oxidefor production nitric oxide (Griess production reagent) (Griess and interleukin reagent) and 6 (DAS interleukin ELISA). 6 (DAS The cells ELISA). wereThe tested cellsfor were tested viability usingfora viability WST1 assay. using aThe WST1 results assay. of this The study results show of that this study Artemisia showafra that is Artemisia very effective afra in is inhibiting very effective the inflammatory in inhibiting activity the inflammatory due to LPS exposure activity due (IC50 to LPS at 170 exposure ug/ml).(IC50 Curcumin, at 170 ug/ml). which isCurcumin, a highly purified which component of turmeric, has anofIC50 of 3 ug/ml. presented NF?ß and sex on steroid is a highly purified component turmeric, has anData IC50 isofalso 3 ug/ml. Data on is also presented NF?ßreceptor and inhibitors on the anti-inflammatory activity of Artemisia afra. Key words: anti-inflammatory activity, Artemisia afra 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-O-024 Cuban Medicinal Plants Journal. Present times and Perspectives Martínez Hormaza, Ioanna ;García Hernández, Ana Ibis Laboratorio central de Farmacología. Hospital Salvador Allende, Cuba [email protected] Introducción: La LaRevista RevistaCubana Cubana de de Plantas Plantas Medicinales Medicinales (RCPM) (RCPM) fundada fundada en 1996, en ofrece 1996, a ofrece los a los profesionales del del campo campodedela la salud salud y sus y sus afines, afines, la posibilidad la posibilidad de publicar de publicar los resultados los resultados de sus de sus investigaciones acerca del del uso usode delas lasplantas plantasmedicinales. medicinales.Expertos Expertosreconocidos reconocidosmedian median el proceso el proceso de de arbitraje. La La RCPM RCPMpretende pretendecontribuir contribuir a laa prevención la prevención de enfermedades de enfermedades y al mejor y al manejo mejor manejo de los de los pacientes, y al al mejoramiento mejoramiento de de lalacalidad calidaddedevida. vida. Objetivo: Analizar el trabajo de la revista durante los Objetivo:5 Analizar el trabajo la revista durante los últimos 5 años. en los últimos 5 años. Con los cuales últimos años. Métodos: se de revisaron todos los números publicados de analizaron estadísticamente principales datos de autores más países y la Métodos: se revisaron todos los los números publicados en la losrevista, últimoslos 5 años. Con lospublicados, cuales de analizaron estadísticamente producción científica los de principales la misma.datos Los datos de la revista, fueron analizados los autores en más una publicados, base de datos países Access. y la producción Resultados: científica los cubanos de la son misma. los autores Los datos que más fueron publican analizados en la en revista una base seguida de datos por Colombia Access.y Brasil. En el año 2013 Resultados: se publicaronlos 60cubanos artículosson en los total autores siendoque el año máscon publican mayoren producción la revista seguida científica.por LaColombia temática ycon Brasil. mayor En el año número de2013 publicaciones se publicaron es Farmacología 60 artículos en y los total Master siendo enelCiencias año conson mayor los que producción más publican científica. en laLarevista. temática con mayor Conclusiones: Los resultados número de demuestran publicaciones la importancia es Farmacología de RCPM y los y que Master se debe en Ciencias incrementar son los la visibilidad que más ennúmero la revista. ypublican elevar el de citas por artículo. Dedicado a: DrC. Francisco José Morón Rodríguez Conclusiones: Los resultados demuestran la importancia de RCPM y que se debe incrementar la visibilidad y elevar el número de citas por artículo. Key words: Cuban Medicinal Plants Journal 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-O-025 Vegetal biotechnology in heterolog proteins production to prevent and treat diseases Calva Calva, Graciano Instituto Politécnico Nacional, México [email protected]; [email protected] Plant-based molecular farming using using transgenic transgenic plants, plants, and and plant plant cell, cell,tissue, tissue,and andorgan organcultures cultureshave havebeen been investigated investigated as alternative as alternative production production platforms platforms for heterologous for heterologous proteins proteins produced produced in mammal, in mammal, microbial, microbial, and insectand cellinsect systems. cell systems. Now commercial Now commercial plant cell plant culture cell culture processes processes includeinclude tobacco tobacco and carrot and cell carrot cell suspension suspension cultures in cultures bioreactors in bioreactors with defined with defined synthetic synthetic medium, medium, and and a number a number of of plantplant-derived derived pharmaceutical pharmaceutical heterologous heterologous proteinsproteins are close aretoclose the market. to the market. Plant systems Plant systems can becan grouped be grouped into three into three categories: categories: seed-based seed-based systems, systems, leaf-based leaf-based systems, systems, and bioreactor-based and bioreactor-based systems. systems. In this In this work, work, plant plant biotechnology biotechnology systems systems are discussed are discussed for the for the production production of of therapeutic therapeutic proteins. proteins.Hairy Hairyroot rootcultures cultures of of Brassica Brassica oleracea oleracea var.var. italica italica (broccoli) (broccoli) areare used used as example as example to evaluate to evaluate the the production production of the of human the human growt h growth hormone hormone (hGH1) (hGH1) or somatotropin, or somatotropin, insulin,insulin, and Human and Human Papilloma Papilloma Virus L1 Virus protein. L1 protein. The major The major advantages advantages of transgenicofroots transgenic are thatroots they are genetically that they are homogeneous, genetically homogeneous, morphologically morphologically stable, and thestable, heterologous and the heterologous is inheritable. Therefore, they are capable synthesizing proteins the protein productionprotein trait is production inheritable. trait Therefore, they are capable of synthesizing theofheterologous heterologous for long periods proteins of time. forOne longofperiods the most of important time. One limitations of the most forimportant the commercial limitations exploitation for the commercial of hairy rootsexploitation based systems of hairy for production roots-based of secondary systems formetabolites production and of secondary heterologous metabolites proteins and has heterologous been the low yields, proteins has and productivity beenthe thedesign low yields, of proper productivity bioreactors and the that design permit of proper both growth bioreactors of thethat rootpermit tissues, bothnormally growth of the rootdistributed unevenly tissues, normally throughunevenly the bioreactor, distributed andthrough expression the bioreactor, of the transgenic and expression product.ofInthe any transgenic case, the product. Inviability economic any case, of the theproduction economic process viability seems of the production to be ensured process by eliminating seems to be lowensured yields and by low eliminating recovery by elite with high expression and by developing scalable processes. lowselecting yields and lowlines recovery by selecting elitelevels, lines with high expression levels, manufacturing and by developing Key words: transgenic protein, agrobacterium rhizogenes, pcambia, gusplus 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-O-026 Isolation and characterization of antiretroviral proteins from Curcuma Longa L. extracts. Results od three multicentric clinical trials in patients with HIV and Hepatitis B and C García Martín, Juan de Jesús; Kaifer, A.; Kaplan, L.; Echegoyen, L. The Curcuma longa L. is a promissory medicinal plant with anti retroviral properties. A new I.J. protein with The longa is a promissory medicinal plant with anti retroviral properties. A new I.J. protein 19KdCurcuma M.W. and two L. isomers is isolated carry through Capilar Zone Electrophoresis, HPLC, IRFT and with 19Kd M.W. and two isomers is isolated carry through Capilar Zone Electrophoresis, HPLC, Circular Dichroism the Convex Algorithm test anti the Secondary sequentialAstructure ofIRFT the ARV The Curcuma longaperforming L. is a promissory medicinal planttowith retroviral properties. new I.J. protein and Dichroism performing Convex Algorithm toCapilar test the Secondary sequential structure ofM.W. the protein. The IJ protein purifiedisthe and freeze dried for further studies. Two isomers or 8 Kd each with Circular 19Kd M.W. and twowas isomers isolated carry through Zone Electrophoresis, HPLC, IRFT ARV appeared protein. seen as intrachain bonded Diketopyperazins. A generic 250 mg Natural Drug Softgels and Circular Dichroism performing the Convex Algorithm to test the Secondary sequential structure of theis performed for Clinical Trials in HIV, Hepatitis B, Hepatitis C. volunteers. The performed trials showed the ARV protein. reduction of the viral charge in freeze HIV, from by studies. ml to 0 Two performing was The IJ protein was purified and dried100,000 for further isomersthe or PCR 8 Kd test. each The M.W.results appeared 89.90% of VCR by bonded ml. In patients with Hepatitis B and C the results were 96.75% VCR respectively. An Ïn seen as intrachain Diketopyperazins The IJ protein was purified and freeze dried for further isomers Kd each appeared Vitro¨Action Mechanism is Done. The IJ protein showsstudies. a fusionTwo inhibition of or the8 virus over M.W. the the Hep-2 seenand as intrachain bonded Diketopyperazins liver HIV Lymphocytes cells surface in Tissue Culture. A generic 250 mg Natural Drug Softgels is performed for Clinical Trials in HIV, Hepatitis B, Hepatitis C. volunteers. Themg performed trials Softgels showed the reduction for of the viral Trials chargeininHIV, HIV,Hepatitis from 100,000 by ml C. to 0 A generic 250 Natural Drug is performed Clinical B, Hepatitis performing the PCR test. The results was 89.90% of VCR by ml. In patients with Hepatitis B and C the Key words: Isolation, characterization, antiretroviral proteins, Curcuma Longa L. clinical trials, patients, volunteers. The performed trials showed the reduction of the viral charge in HIV, from 100,000 by ml to 0 , Hepatitis B results were 96.75% VCR respectively. .HIV An Ïn Vitro¨Action Mechanism is Done. The IJ protein shows a fusion inhibition of the virus over the the 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-O-027 Potentialities of the use of lipdic extract from Cuban Royal palm fruits in China population Hongying, Li WEGO, Canada [email protected] (HPB) es una que puede los hombres La Hiperplasia Hiperplasia Prostática ProstáticaBenigna Benigna (HPB) es patología una patología que afectar puede a afectar a los hombres 50años años yycuya cuyafrecuencia frecuencia aumenta aumentacon conlalaedad. edad.En Enchina chinaseseestima estimaque quetengan tenganesta fundamentalmente 50 esta afectación más25demillones 25 millones de hombres el 2020. Esta patología consiste un afectación más de de hombres hastahasta el 2020. Esta patología consiste en un en agrandamient o agrandamiento benignoque de puede la próstata queobstrucción puede causar obstrucción y propicia el desarrollo síntomas benigno de la próstata causar y propicia el desarrollo de síntomas del de tracto bajo del tractoentre bajo los urinario entre los que destacandel disminución delorina volumen de orinade y la la urinario que destacan disminución volumen de y la presión la presión micción,deretención micción, y aumento la nicturia. De acuerdo al grado la deintensidad obstrucción, intensidad urinaria yretención aumentourinaria de la nicturia. De de acuerdo al grado de obstrucción, delalos síntomasde los síntomas variar, llegando a afectar calidad de hombres vida de los que laSepadecen. puede variar, puede llegando a afectar la calidad delavida de los quehombres la padecen. plantea Se que su plantea queresponde su desarrollo responde a cambios hormonales y no que ocurren durante el El desarrollo a cambios hormonales y no hormonales quehormonales ocurren durante el envejecimiento. envejecimiento. El aumento dede la la transformación de en la testosterona (T) en dihidrotestosterona a aumento de la transformación testosterona (T) dihidrotestosterona (DHT), a través de la(DHT), enzima través de la enzima 5 -reductasa prostática, el principal cambio hormonal y el no principal 5 -reductasa prostática, es el principal cambioes hormonal asociado y el principal asociado componente hormonal componente porlael musculatura aumento del lisa tonode delalavejiga musculatura lisa de la vejiga ypor la los está dado pornoelhormonal aumentoestá del dado tono de y la próstata, regulado próstata, regulado (ADR)por los 1.. adrenoreceptores (ADR)1.. La principal de inhibidores la HPB la adrenoreceptores La principal terapia farmacológica de laterapia HPB lafarmacológica constituyen los constituyen los inhibidores delos la 5 -reductasa prostática, de ADR y la terapia de la 5 -reductasa prostática, antagonistas de ADR -1, los y laantagonistas terapia combinada con-1, ambos, todos ellos combinada ellos asociados un númerocomo importante de efectos la asociados acon un ambos, número todos importante de efectosa adversos la disminución de adversos la líbido, como impotencia, disminución impotencia, trastornos ortostática. de la eyaculación, la hipotensión En china, trastornos dedelala líbido, eyaculación, la hipotensión En china, con largaortostática. tradición del empleo con larga tradición del empleo etnomédico de productos existen como diferentes formulaciones etnomédico de productos naturales, existen diferentesnaturales, formulaciones baishu, dangsheng, como baishu, dangsheng, danggui, ect., variables, éxito deque su uso bajo, mientras danggui, ect., son muy variables, pero el son éxitomuy de su uso es pero bajo, el mientras en elesmundo occidental que en el el mundo mundo occidental occidental el extracto extractoha lipídico lipídico de Sawplametto plametto ha hasido sidolalaalternativa alternativa fitoterapéutica fitoterapéutica más más el extracto lipídico de Saw el plametto sido de la Saw alternativa fitoterapéutica más comúnmente usada. comúnmente se han handeencontrado encontrado reporteslípido de un unobtenido nuevo nuevo extracto extracto lípido lípido obtenido obtenido de Recientementeusada. se hanRecientemente encontrado reportes un nuevo extracto de la palma real cubanade la palma realregia), cubana regia), con amplia información avalan su eficacia en (Roystonea con(Roystonea amplia información preclínica que avalan preclínica su eficaciaque en modelos experimentales modelos experimentales y su la par que los ensayos clínicos muestran y su seguridad toxicológica a seguridad la par que toxicológica los ensayosaclínicos muestran resultados prometedores. Este resultados prometedores. Esteuna extracto está por una mezcla de ácidos grasos, principalmente extracto está constituido por mezcla deconstituido ácidos grasos, principalmente oleico, palmitico, láurico, oleico, láurico, linoleico mirístico,de y previene el desarrollo de roedores la HP inducida con los T encambios linoleicopalmitico, y mirístico, y previene el ydesarrollo la HP inducida con T en así como roedores así ycomo los cambios y urodinámicos queinducida caracterizan a la HP atípica inducida por histológicos urodinámicos quehistológicos caracterizan a la HP atípica por fenilefrina, efectos que se fenilefrina, se relacionan una inhibición la 5 -reductasa y una inhibición de laspor los relacionan efectos con unaque inhibición de la 5con -reductasa y una de inhibición de las respuestas mediadas respuestas mediadas por los 1. Además presenta moderada acción anti- a la adrenoreceptores (ADR)1. adrenoreceptores Además presenta(ADR)moderada acción anti- inflamatoria, asociada inflamatoria, asociada a la inhibición dual de las enzimas ciclooxigenasa 5-lipooxigenasa (5inhibición dual de las enzimas ciclooxigenasa (COX) y 5-lipooxigenasa (5-(COX) LOX). yEstudios en voluntarios LOX). enbien voluntarios mostró ser tolerado, que sin producir las EA, mientras en sanos Estudios mostró ser tolerado,sanos sin producir las bien EA, mientras en pacientes sanos y con que HP mostró pacientes sanos y con Por HP mostró efectos antioxidantes. Por durante otra parte de fue 320tan mg/día durante 4y efectos antioxidantes. otra parte a dosis de 320 mg/día 4 ya 6dosis meses efectivo como 6 tan efectivo comolos el STBU SP (320 mg/día) en reducir la losreducción STBU evaluados mediante la reducción el meses SP (320fuemg/día) en reducir evaluados mediante del puntaje IPSS, produciendo del puntaje produciendo un descenso modesto volumen prostático. Unase variante de además un IPSS, descenso modestoademás del volumen prostático. Una del variante de este producto encuentra este producto sesuplemento encuentra registrado suplemento nutricional en Cuba con el nombre de Palmex, registrado como nutricionalcomo en Cuba con el nombre de Palmex, que dado la información que que dado la pudiera información quecandidato lo sustenta pudiera ser un tomado candidato a sercomo tomado lo sustenta ser un importante a ser enimportante consideración unaenalternativa consideración como una en el Benigna manejo de Prostáticachina. Benigna en la natural en el manejo de laalternativa Hiperplasianatural Prostática en la la Hiperplasia población masculina Key words: lipdic extract, Cuban Royal palm fruit, China, IPB 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-O-028 In-vitro anthelmintic activity of Mucuna pruriens (DC) and Canarium schweinfurthii (Engl) on Acaris suum Habila, James D.; Okoli, B. J.; Ayo, R. G.; Japhet, S. L.; Ndukwe, G. I. Department of Chemistry, Ahmadu Bello University, Nigeria [email protected] Helminth infections are among the most widespread infections in humans, especially especially in in the the tropical tropical regions regions of the world. of theItworld. causes It causes enormous enormous hazard hazard to health to health and contributes and contributes to the to prevalence the prevalence of malnourishment of , malnourishment, anemia and pneumonia anemia among and pneumonia others. Mucuna among pruriens others. Mucuna and Canarium pruriensschweinfurthii and Canariumused schweinfurthii in traditional used in traditional medicinal practicesmedicinal in Northern practices Nigeria, in Northern for the treatment Nigeria, for of worms the treatment infestation of worms were investigated. infestation were The ininvestigated. vitro anthelmintic The evaluation in-vitro anthelmintic of the crude evaluation and solvent of the partitions crude and extracts, solventonpartitions Pheretima extracts, pothuma onand Pheretima Ascaris pothuma suum (eggs andand Ascaris L2 stage) suumrespectively. (eggs and L2Showed stage) respectively. that the hexane Showed fraction that the(leaves hexaneand fraction stem bark) (leaves of C. and stem bark)and schweinfurthii of C. Mucuna schweinfurthii pruriens and (leaves) Mucuna had pruriens ovicidal potency (leaves) of had (100.00 ovicidal ±0.33) potency at 80mg/ml of which was (100.00 ±0.33) comparable to at the 80mg/ml positivewhich control; was Albendazole(100.00 comparable to the positive ±0.33) control; at the Albendazole(100.00 same concentration.±0.33) Whileatthe the same concentration. Chloroform fractions showed Whileovicidal the Chloroform activity offractions 98.20±0.12 showed and ovicidal 98.20± activity 0.12 80ofmg/ml 98.20±0.12 on theand two98.20± plants 0.12 80 mg/ml respectively. The onresults the twoofplants determination respectively. of larvicidal The results potency of determination showed that the of larvicidal hexane fraction potency(0.06%) showedwas that most the the hexane potent,fraction followed(0.06%) by thewas Chloroform the mostfractions potent, followed (0.1 andby 0.2the %Chloroform respectively). fractions These(0.1 results and clearly 0.2 % respectively). justified the uses These of these results plants clearly in the justified treatment the uses of worms of these in ethnoplants medicinal in the treatment practices. of worms in ethno- Key words: anthelmintic activity, mucuna pruriens, canarium schweinfurthii, pheretima pothuma, a. suum, 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-O-029 Establishment of septic culture of Adenophyllum aurantium Sánchez Espinosa, Ana Claudia; Cruz, Jorge Alberto; Calderón Oropeza, Mónica Alicia; Ramírez Hernández, José Eduardo The role and variety control.Such variety of ofchemical chemicalcompounds compounds ininplants plantsmake makethem thempotential potentialcandidates candidatesforforpest pest is the case ofisAdenophyllum aurantium (L.) Strother,whose root extract root containing acetate reduced control.Such the case of Adenophyllum aurantium (L.) Strother,whose extractethyl containing ethyl the mycelial growth sporulation of phytopathogenic fungusFusariumfungusFusarium solani and Alternaria acetate reduced the and mycelial growth and sporulation of phytopathogenic solani alternata, and Alternaria without harming alternata, the growth withoutofharming the biofertilizer the growth Bacillus of thelicheniformis. biofertilizer Bacillus Biotechnological licheniformis. techniques Biotechnological have been techniques used to obtain havenatural been products used to obtain that are, natural for some products reason, that hard are, for to obtain some reason, at largehard scaletothrough obtain at chemical large scale through synthesis. Saidchemical techniques synthesis. are useful Saidtotechniques preserve germplasm; are useful to additionally, preserve germplasm; they help toadditionally, prevent excessive they help to prevent predation of wild excessive populations predation whichofmaintains wild populations ?to a certain which maintains extent? biodiversity ?to a certainand extent? genebiodiversity stability of and gene stability species.Due to theofabove, species.Due strategies to the to above, obtain astrategies large number to obtain of individuals a large number for its of use individuals in phytochemical, for its use in phytochemical, structural and metabolic structural studiesareimportant. and metabolic studiesareimportant. Preliminary resultsPreliminary show thatresults we obtained show that 95% we of obtained 95% of establishment of establishment aseptic cultures of aseptic from seeds. cultures Micro-cutting from seeds.were Micro-cutting sectionedwere from sectioned germinated from seedlings, germinated and the induction seedlings, of multiple and thesprouts induction fromof axillary multiplebuds sprouts wasfrom achieved axillary using budsMS wasmedium achieved (Murashige using MSand Skoog, supplemented with BAPsupplemented (2.5 milligrams perBAP litre) and AIA (0.1per milligrams medium 1962) (Murashige and Skoog, 1962) with (2.5 milligrams litre) andper AIAlitre).Root (0.1 forming occurred spontaneously in 90% of the micro-cuttings. Key words: adenophyllum aurantium, micropropagation, medicinal plant 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-O-030 Round Table: 25 years validation of the medicinal plants use Duménigo González, Abel; Victoria Amador, María del Carmen; López Barreiro, Marisol; Brito Álvarez, Gisselle; García Mesa, Milagros; Acosta de La Luz, Lérida; Blanco Hernández, Yisel; Morejón Rodríguez , Zulema; García Hernández, Ana Ibis; Martínez Hormaza, Ioanna Hospital Salvador Allende, Cuba [email protected] Introducción: Introducción: Desde Desde 1988 1988 el el Laboratorio Laboratorio Central Central de deFarmacología Farmacología (LCF) (LCF)apoyada apoyadapor porelelMinisterio MinisteriodedeSalud Pública (MINSAP), ha trabajado en las investigaciones los usos de plantas medicinales con objetivo Salud Pública (MINSAP), ha trabajado en las investigaciones los usos de plantas medicinaleselcon el de incorporarlas en el Sistema Nacional de Salud. Además ha coordinado el Programa Cubano objetivo de incorporarlas en el Sistema Nacional de Salud. Además ha coordinado el Programa Cubanode Investigaciones de de Plantas Medicinales de Investigaciones Plantas Medicinales(1988-2004). (1988-2004).Objetivo: Exponer el trabajo realizado durante 25 años del Exponer LCF en el la trabajo validación del uso tradicional plantas se revisaron Objetivo: realizado durante 25 añosdedel LCF enmedicinales. la validaciónMétodos: del uso tradicional de los principales resultados, trabajos de difusión y la docencia medica superior, así como los resultados plantas medicinales. publicados los incluidos en la Farmacopea de yTRAMIL desde 1988superior, hasta 2014. Métodos: seyrevisaron los principales resultados,Vegetal trabajosCaribeña de difusión la docencia medica así Resultados: se expone el trabajo realizado con plantas científicamente validadas durante 25 como los resultados publicados y los incluidos en la Farmacopea Vegetal Caribeña de TRAMILaños, desdeen el cual un elevado porcentaje de usos tradicionales respaldados por evaluaciones experimentales 1988 existe hasta 2014. preclínicas, ratifica el la trabajo ausencia casi total deplantas toxicidad en las formas validadas de uso tradicional. presenta Resultados: que se expone realizado con científicamente durante 25Seaños, en el el trabajo de colaboración con la red TRAMIL para la difusión del uso tradicional, su eficacia y seguridad cual existe un elevado porcentaje de usos tradicionales respaldados por evaluaciones experimentales en Cuba y el Caribe. Conclusiones: Los datos la importancia de lade validación del usoSe tradicional preclínicas, que ratifica la ausencia casi totalindican de toxicidad en las formas uso tradicional. presenta de plantas, la adecuada divulgación de los resultados y la necesidad de establecer una metodología adecuada el trabajo de colaboración con la red TRAMIL para la difusión del uso tradicional, su eficacia y seguridad para la validación. en Cuba y el Caribe. Conclusiones: Los datos indican la importancia de la validación del uso tradicional de plantas, la adecuada la Key words: plantas medicinales, farmacopea vegetal caribeña, fitoquímica, farmacología, microbiología, 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-001 Effects of policosanol pre-treatment on blood-brain barrier damage and inflammatory markers after ischemia-reperfusion brain injury in rats Pérez Guerra, Yohani; Oyarzábal Year, Ambar; Mas Ferreiro, Rosa María; Molina Cuevas, Vivian; Jiménez Despaigne, Sonia Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] Blood-brain barrier (BBB) disruption following ischemia-reperfusion (I/R) is associated with poor outcomes in stroke patients. a mixture of sugarcane wax alcohols, hasis been shownwith to protect Blood-brain barrier Policosanol, (BBB) disruption following ischemia-reperfusion (I/R) associated poor against cerebral histological disturbances by I/R wax in gerbils. Nevertheless, the effects of outcomesfunctional in stroke and patients. Policosanol, a mixtureinduced of sugarcane alcohols, has been shown to policosanol on experimentally induced disruption have not been explored. objective of this study protect against cerebral functional and BBB histological disturbances induced by I/R The in gerbils. Nevertheless, was to investigate the effects of policosanol on BBB disruption following induction of cerebral the effects of policosanol on experimentally induced BBB disruption havethenot been explored. TheI/R in rats. Rats wereofrandomized intotoa investigate negative vehicle control five I/R on groups: a positive vehicle three objective this study was the effects of and policosanol BBB disruption followingcontrol, the policosanol 200 I/R andin400 one randomized aspirin (150 into mg/kg). Treatments given 1 hgroups: before induction of (50, cerebral rats.mg/kg), Rats were a negative vehiclewere control andorally five I/R ischemia Brain control, ischemia was policosanol induced by (50, 30 of carotid arteries, followed groups: a positivea induction. positive vehicle control, vehicle three policosanol three (50, 200 andmin 200 400bilateral and mg/kg), 400occlusion one mg/kg), aspirin one (150 aspirin mg/kg). (150 mg/kg). by 24 h reperfusion. BBB permeability was evaluated according to Evans bluewas (EB)induced dye extravasation. Treatments were given orally 1 h before ischemia induction. Brain ischemia by 30 min Also, myeloperoxidase activity in brain homogenates was measured. concentration MPO values bilateral occlusion(MPO) of carotid arteries, followed by 24 h reperfusion. BBBEB permeability was and evaluated after 24 h of significantly increased positive controls (MPO) when compared according to reperfusion Evans blue were (EB) dye extravasation. Also,inmyeloperoxidase activity in with brainthe negative controls. Oral was pre-treatment policosanol (50 - 400 extravasation homogenates measured.with EB concentration and MPOmg/kg) values significantly after 24 h ofdecreased reperfusionEB were (60.4% - 71.7%) as compared to the positive controls. Aspirin reduced significantly BBB leakage by 92.5%. significantly increased in positive controls when compared with the negative controls. Oral pre-treatment Also, the I/R-induced increases MPO activities were EB lowered significantly with- policosanol (50 - 400 with policosanol (50 - 400 mg/kg)ofsignificantly decreased extravasation (60.4% 71.7%) as compared mg/kg) (46.3%controls. - 65.3) and aspirin (68.4%). In conclusion, oral policosanol (50 - 400 mg/kg) to the positive Aspirin reduced significantly BBB leakage by 92.5%.pre-treatment Also, the I/R-induced protected damage cerebral I/R rats, and attenuated the increase MPO, a increases against of MPO BBB activities were induced lowered by significantly withinpolicosanol (50 - 400 mg/kg) (46.3% of- 65.3) marker of inflammation, in the brain oral tissue. and aspirin (68.4%). In conclusion, policosanol pre-treatment (50 - 400 mg/kg) protected against BBB Key words: policosanol, blood-brain barrier damage, inflammatory markers, ischemia-reperfusion, brain injury, rat 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-002 Comparative study of the preventive effects of Policosanol and the omega-3 fatty acid of fish oil in models of cerebral ischemia Mena Valdés, Licet ;Molina Cuevas, Vivian; Ravelo Calzado, Yazmin; Noa Puig, Miriam; Valle Clara, Maikel; Pérez Guerra, Yohani; Oyarzábal Year, Ambar; Mas Ferreiro, Rosa María; Mendoza Hernández , Nilda; Jiménez Despaigne, Sonia Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] Stroke is the third cause of death and the first of permanent adult disability. Pre-treatment with policosanol and omega-3 fish oil (FO)ofhas been models of cerebral ischemia with in rodents. This Stroke is the third cause death andeffective the firstinofexperimental permanent adult disability. Pre-treatment study compared the effectsfish of policosanol oil in the global cerebral ischemiaischemia (GCI) model policosanol and omega-3 oil (FO) has and beenomega-3 effectivefish in experimental models of cerebral in induced by ischemia-reperfusion (I/R) inofMongolian Gerbils fish were distributed in 7 rodents. This study compared the effects policosanolgerbils. and omega-3 oil randomized in the global cerebral groups: negative control group and 6 with GCI: a positive (vehicle), policosanol (100 and 200 ischemiaa(GCI) model induced by ischemia-reperfusion (I/R) incontrol Mongolian gerbils. Gerbils were mg/kg) and FO (1.25 and g/kg g/kg) and aspirin mg/kg). treatments were administered by oral randomized distributed in 72.5 groups: a negative control(60 group and All 6 with GCI: a positive control (vehicle), route during(100 4 weeks. Policosanol (100 200and mg/kg) andg/kg) FO (1.25 and 2.5(60 g/kg) significantly reduced policosanol and 200 mg/kg) and FOand (1.25 2.5 g/kg and aspirin mg/kg). All treatments the symptoms score (63.6during and 73.3%; 32.7 and 58.8%, respectively), the rate clinical wereclinical administered by oral route 4 weeks. Policosanol (100 and 200 mg/kg) andofFO (1.25symptoms and 2.5 (60 y 60%; 33.4 y 36.4%, the hyper-locomotion y 82.5%; 26.7 y 100%, g/kg) significantly reducedrespectively), the clinical symptoms score (63.6 (84.4 and 73.3%; 32.7 and 58.8%,respectively), respectively),the histological score symptoms of neurological (41.4 y 52.1%; 28.3 y 32.6 respectively) and(84.4 the yplasmatic the rate of clinical (60 y damage 60%; 33.4 y 36.4%, respectively), the%, hyper-locomotion 82.5%; levels malondialdehyde y 83.3%; 59.9 y 72.7%, respectively) and(41.4 sulphydril groups y 100%, 26.7 y of 100%, respectively),(76.7 the histological score of neurological damage y 52.1%; 28.3(100 y 32.6 %, respectively). In conclusion, Policosanol (100 and 200 mg/kg) FO (1.25 2.5 g/kg) markedand and respectively) and the plasmatic levels of malondialdehyde (76.7 yand 83.3%; 59.9 yand 72.7%, respectively) significantly reduced GCI in respectively). Mongolian gerbils, with a mayor potency (100 and efficacy Policosanol. sulphydril groups (100the y 100%, In conclusion, Policosanol and 200ofmg/kg) and FO (1.25 and 2.5 g/kg) marked and significantly reduced the GCI in Mongolian gerbils, with a mayor potency Key words: policosanol, omega-3 fish oil, cerebral ischemia, mongolian gerbils 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-003 Long term stability study of D002 active ingredient Morales Rico, Carmen Luisa; Sierra Pérez, Roxana de la Caridad; González Canavacciolo, Víctor L.; Vicente Murillo, Roxana; Rodríguez Leyes, Eduardo Antonio; Marrero Delange, David Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] El D002 es una mezcla de alcoholes grasos grasos (C24 (C24 aa C34) C34) extraídos extraídos yy purificados purificados de de la la cera cerade de abejas abejas Apis mellifera, el cual constituye el ingrediente activo (IA) BWA®las lascuales cuales Apis mellifera, el cual constituye el ingrediente activo (IA)dedelas lastabletas tabletas de de Abexol® yy BWA® presentan efectos antioxidantes yy gastroprotectores. gastroprotectores. El Elpresente presente estudio estudioevaluó evaluó lalaestabilidad estabilidaddede3 3lotes lotes almacenados bajo condiciones de degradación forzada, estabilidad acelerada (40ºC y 75% 75% HR) yy a largo largo plazo (3 (3 años) años)enenlaslaszonas zonas climáticas IV. parámetros Los parámetros medidos en los todos los fueron: casos fueron: climáticas II y IIIV.y Los medidos en todos casos características organolépticas, contenido contenido (%) (%)total totaleeindividual individualdedealcoholes alcoholes mediante cromatografía mediante cromatografía de de gases con columna capilar, aparición aparición de de productos de de degradación degradación así así como como las las pérdidas pérdidas por por secado secado en en los casos de de la la estabilidad estabilidad acelerada acelerada yyaalargo largoplazo. plazo.Los Losresultados resultadosmuestran muestranque quebajo bajocondiciones condicionesdede estrés (hidrólisis ácida y básica, oxidación, termólisis y fotólisis) no se formaron productos de de degradación ydegradación se mantuvo yelseporcentaje deporcentaje pureza superior al 87%. Por su los estudios mantuvo el de pureza superior al parte, 87%. Por su parte, de losestabilidad estudios deacelerada (12 meses) acelerada y a largo plazo demostraron la plazo elevada estabilidad ladeelevada estos alcoholes. no se estabilidad (12 meses) y a largo demostraron estabilidadAdicionalmente, de estos alcoholes. observó, bajo ninguna de lasbajo condiciones ensayadas, cambios de coloración extraños, Adicionalmente, no se observó, ninguna de las condiciones ensayadas, cambios ni de olores coloración ni permaneciendo IA como un polvo deun consistencia cerosa y color blanco Las pérdidas olores extraños, el permaneciendo el IA fino como polvo fino de consistencia cerosa hueso. y color blanco hueso. por secado fueronporinferiores al 1% inferiores lo cual sealcorresponde las especificaciones de calidad para Las pérdidas secado fueron 1% lo cual secon corresponde con las especificaciones de dicho producto. Losdicho resultados del presente estudiodel confirman tiempoconfirman de vida útilundetiempo 3 años,delovida cualútil concuerda calidad para producto. Los resultados presenteunestudio de 3 con losloinformados en estudios de estabilidad delprevios D002 ydesus formas terminadas. años, cual concuerda con los previos informados en estudios estabilidad del D002 y sus formas Key words: d002, alcoholes de la cera de abejas, alcoholes grasos, estabilidad 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-004 Long term stability study of 20 mg Policosanol tablets in blisters Sierra Pérez, Roxana de la Caridad; González Canavacciolo, Víctor L.; Vicente Murillo, Roxana; Rodríguez Leyes, Eduardo Antonio; Marrero Delange, David Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] El policosanol constituye una mezcla reproducible reproducible de de ocho ocho alcoholes alcoholes grasos grasos primarios primarios de de 24 24 aa 34 34átomos átomos de carbono carbonopurificados purificados la cañaEsta desustancia, azúcar. con Esta sustancia, con efectos de lade ceralade cera la cañadede azúcar. efectos hipocolesterolemizantes y antiagregante plaquetarios, plaquetarios, es es empleada empleada en enlalaelaboración elaboración de detabletas tabletascon con2020 mg de alcoholes, forma farmacéutica terminada utilizada en los ensayos clínicos y en la práctica de los clínicos y en la práctica de rutina. El objetivo fue determinar la estabilidad de estas tabletas en blísteres de cloruro de polivinilo y aluminio. rutina. El objetivo fue determinar la estabilidad de estas tabletas en blísteres de cloruro de polivinilo y Muestras de tres lotes de tabletas se Cuba) pusieron cajas de mantuvieron en las aluminio. Muestras de tres lotes de(MedSol, tabletas Cuba) (MedSol, seen pusieron encartón cajas ydesecartón y se condiciones climáticadeIVla(30 C, 70 IV 5% durante relativa) cinco años. Los mantuvieron de en la laszona condiciones zona2climática (30de2humedad C, 70 5 relativa) % de humedad durante parámetros se mantuvieron de sus especificaciones de calidad durante el estudio: cinco años. evaluados Los parámetros evaluados dentro se mantuvieron dentro de sus especificaciones de todo calidad apariencia (tabletas verdes con superficies peso promedioenteras), (inicial peso 10 %),promedio contenido total de10los durante todo el estudio: apariencia (tabletas enteras), verdes con superficies (inicial seis alcoholes total grasos ± 2 mg), tiempograsos de desintegración 30 min), y contenido microbiológico %), contenido de (20 los seis alcoholes (20 ± 2 mg), (< tiempo de desintegración (< 30 min), y (1000 bacterias/g y ? 100 hongos/g, ausencia de E. coli, S. aureus, Pseudomonas, C. albicans). Los contenido microbiológico (? 1000 bacterias/g y ? 100 hongos/g, ausencia deSalmonella E. coli, S. yaureus, resultados del Salmonella presente estudio sustentan las tabletas de policosanol con dosis detabletas 20 mg, Pseudomonas, y C. albicans). del presente estudio sustentan que las Los que resultados almacenadas lasdosis condiciones de la zona climática tiempo de climática vida útil de años. de policosanolencon de 20 mg, almacenadas en IV, las presentan condicionesunde la zona IV,cinco presentan Key words: policosanol, cera de caña de azúcar, alcoholes grasos, estabilidad, tabletas 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-005 Stability of Abexol® tablets in blisters and high density polyethylene flasks González Canavacciolo, Víctor L.; Sierra Pérez, Roxana de la Caridad; Morales Rico, Carmen Luisa; Vicente Murillo, Roxana; Rodríguez Leyes, Eduardo Antonio; Marrero Delange, David Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] Los alcoholes de la la cera cera de de abejas abejas constituyen constituyenuna unamezcla mezclareproducible reproducible de deseis seisalcoholes alcoholesgrasos grasosprimarios primarios de 24 a 34 de átomos 24 a 34 átomos de carbono de carbono purificados purificados de la cera de la de ceraApis de Apis mellifera. mellifera. EstaEsta sustancia, sustancia, concon efectos efectos antioxidantes y gastroprotectores, es empleada la elaboración de las tabletas de Abexol® antioxidantes y gastroprotectores, es empleada para la para elaboración de las tabletas de Abexol® con dosis con dosis de de alcoholes, 50mg de alcoholes, forma farmacéutica utilizada en los clínicos ensayosy clínicos y en lade de 50mg forma farmacéutica terminada terminada utilizada en los ensayos en la práctica prácticaElde rutina.fue El objetivo fue la determinar la de estabilidad de las 50 mg dede alcoholes rutina. objetivo determinar estabilidad las tabletas contabletas 50 mg con de alcoholes cera dede abejas cera de abejas (Abexol®) enfrascos blísteres en frascos de dealta polietileno de Muestras alta densidad. Muestras detabletas tres lotes (Abexol®) en blísteres y en dey polietileno densidad. de tres lotes de de de tabletas Abexol® con dedosis Abexol® de 50mg con dosis envasadas de 50mgenenvasadas blísteres de en cloruro blísteresdedepolivinilo cloruro de y aluminio polivinilo yy en aluminio frascos y en de frascos de de polietileno polietileno alta densidad de alta (Rainbow densidad & (Rainbow Nature, & Sydney, Nature, Australia) Sydney, Australia) se pusieron se pusieron en cajas en de cajas cartóndey se cartón y se mantuvieron mantuvieron en las condiciones en las condiciones de la zona climática de la zonaIVclimática (30 2 C, IV (30 70 52%C,de70humedad 5 % de relativa) humedaddurant e relativa) cinco años. durante En ambos cinco años. envases, En los ambos parámetros envases,evaluados los parámetros se mantuvieron evaluadosdentro se mantuvieron de sus especificaciones dentro de suscalidad de especificaciones durante todo de calidad el estudio: durante apariencia todo el(tabletas estudio: apariencia blancas con (tabletas superficies blancas enteras), con superficies peso promedio enteras),7,5 (inicial peso %),promedio contenido(inicial total de7,5 los%), seis contenido alcoholes total grasos de los(50 seis ± 3,75 alcoholes mg), grasos tiempo (50 de desintegración ± 3,75 mg), (< tiempo 15 min),dedureza desintegración (> 3 kg/cm2) (< 15y min), contenido dureza microbiológico (> 3 kg/cm2) y(?contenido 1000 bacterias/g microbiológico y ? 100 (? hongos/g, ausencia 1000 bacterias/g y ? E. de 100coli, hongos/g, S. aureus, ausencia Pseudomonas, de E. coli, S.Salmonella aureus, Pseudomonas, y C. albicans). Salmonella Los resultados y C. albicans). del presente Los estudio resultados que sustentan del las presente tabletas estudio de Abexol® sustentan conque dosis las de tabletas 50mg,de envasadas Abexol® en conambos dosis de envases, 50mg, envasadas almacenadas en las ambos condiciones envases, dealmacenadas la zona climática en las IV,condiciones presentan un de tiempo la zonade climática vida útilIV, de presentan cinco años. un tiempo de Key words: abexol, alcoholes de la cera de abejas, alcoholes grasos, estabilidad, tabletas 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-006 Inhibition of ciclooxygenase and 5-lipooxygenase enzymes by D-002 (beeswax alcohols) Pérez Guerra, Yohani; Oyarzábal Year, Ambar; Mas Ferreiro, Rosa María; Molina Cuevas, Vivian; Jiménez Despaigne, Sonia Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] D-002, a mixture of six higher aliphatic beeswax alcohols, has been shown to produce anti-inflammatory effects experimental modelsaliphatic and to exhibit gastroprotective, not gastrotoxic effects. The mechanism of D-002, in a mixture of six higher beeswax alcohols, has been shown to produce anti-inflammatory the anti-inflammatory D-002, however, had not been yet.effects. This study was aimed of at effects in experimentalaction modelsofand to exhibit gastroprotective, notexplored gastrotoxic The mechanism investigating the effects of D-002 on cyclooxygenase and 5- lipoxygenase (5-LOX) activities in the anti-inflammatory action of D-002, however, had not(COX) been explored yet. This study was aimed at vitro. The addition of D-002 to preparations of the (COX) cytosolic of rat blood polymorphonuclear investigating the effects of D-002 on cyclooxygenase andfraction 5- lipoxygenase (5-LOX) activities in leukocytes significantly (p<0.001) and dose-dependently inhibited thepolymorphonuclear in vitro activity of 5-LOX vitro. The addition of D-002 to preparations of the cytosolic(r=0.990) fraction of rat blood up to 81% significantly at 500?g/mL concentration. In contrast, nordihydroguaiaretic the of referenc leukocytes (p<0.001) and dose-dependently (r=0.990) inhibited acid the in(NDGA), vitro activity 5-LOXe substance, 5-LOX activity by In55%. Also,nordihydroguaiaretic D- 002 produced a acid significant (p<the 0.001) and doseup to 81% atinhibited 500?g/mL concentration. contrast, (NDGA), reference dependent inhibition of COX activity in preparations of rataliver microsomes up toand 61%doseat 2500 substance, (r=0.984) inhibited 5-LOX activity by 55%. Also, D- 002 produced significant (p< 0.001) ? g/ml, while(r=0.984) indomethacin at 0.4 produced a 90% inhibition. D-002 bothup kinetic parameters dependent inhibition ofmg/ml COX activity in preparations of rat liver modified microsomes to 61% at 2500 ? (Vmax andindomethacin Km) of 5-LOX andmg/ml COXproduced activities athrough uncompetitive mode ofboth inhibition. This study g/ml, while at 0.4 90% inhibition. D-002 modified kinetic parameters demonstrates that D-002 inhibits both COX and 5-LOX activities, with a highest affinity This for 5-LOX, (Vmax and Km) of 5-LOX and COX activities through uncompetitive mode of inhibition. study so that the dual inhibition both inhibits enzymeboth activities may 5-LOX explainactivities, the observed D-002 demonstrates that of D-002 COX and with aanti-inflammatory highest affinity foreffects 5-LOX,of so that as the lackofofbotheffects D- 002-related gastrotoxicity. thewell dualasinhibition of D-002 Key words: ciclooxygenase and 5-lipooxygenase, enzymes, D-002, beeswax, alcohols 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-007 Effects of D-002 (beeswax alcohols), grape seed extract and their combined therapy on oxidative markers in rat plasma and liver Oyarzábal Year, Ambar Molina Cuevas, Vivian; Mas Ferreiro, Rosa María; Jiménez Despaigne, Sonia; Curveco, Dayisell Lázara Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] D-002, a mixture of high molecular weight alcohols from beeswax (Appis mellifera), and grape seed extract (Vitis L), of rich in flavonoids, presents antioxidant no previous study had compared D-002,vinifera, a mixture high molecular weight alcohols from effects, beeswaxbut(Appis mellifera), and grape seed such effects. The aim of thisL),study was to investigate the effects of D-002, grape extract and their no previous study hadcombined extract (Vitis vinifera, rich in flavonoids, presents antioxidant effects, butseed therapy on such rat plasma and liver markers. experiments conducted. rats compared effects. The aimoxidative of this study was Two to investigate the were effects of D-002, First grapeexperiment: seed extract were distributed intotherapy seven groups: a vehicle control, three D-002-treated, grape seed extract-treated and their combined on rat plasma and liver oxidative markers. Twothree experiments were conducted. (25, and 250 mg/kg/day, respectively). Secondgroups: experiment: rats control, were randomized into four groups: into seven a vehicle three D-002-treated, three one First100 experiment: rats were distributed vehicle control and three treated withand D-002, seed respectively). extract or theirSecond combined therapy (25 grape seed extract-treated (25, 100 250 grape mg/kg/day, experiment: ratsmg/kg/day). were Treatments werefour given orallyone for vehicle 30 days. In the first experiment: D- D-002, 002 andgrape grape seed extract (25randomized into groups: control and three treated with seed extract or their 250mg/kg/day) plasma Treatments malondialdehyde, totalorally hydroperoxides, groups andD-liver combined therapyreduced (25 mg/kg/day). were given for 30 days. Incarbonyl the first experiment: malondialdehyde formation with similar efficacy, but unchanged blood superoxide dismutase. Second 002 and grape seed extract (25-250mg/kg/day) reduced plasma malondialdehyde, total hydroperoxides, experiment: each monotherapy produced significant and efficacy, comparable reductions carbonyl groups and liver malondialdehyde formation with similar but unchanged bloodof plasma malondialdehyde, carbonyl groups and livereach malondialdehyde The combined therapy lowered superoxide dismutase. Second experiment: monotherapy formation. produced significant and comparable plasma was more effectively each monotherapy, formation. and reduced liver reductionsoxidative of plasmavariables malondialdehyde, carbonyl groups than and liver malondialdehyde The malondialdehyde thanplasma grape oxidative seed extract, not than D-002.effectively In conclusions D-002 and grape and seed combined therapy more lowered variables was more than each monotherapy, extract mg/kg/day) significantly and similarly reduced plasma oxidative variables and and liver reduced (25-250 liver malondialdehyde more than grape seed extract, not than D-002. In conclusions D-002 malondialdehyde without modifying blood superoxide dismutase. combined therapy grape seed extractformation, (25-250 mg/kg/day) significantly and similarly reduced plasma The oxidative variables and displayed benefits overformation, each monotherapy regarding to plasma oxidative variables, liver malondialdehyde without modifying blood superoxide dismutase. The whereas combinedregarding therapy to liver malondialdehyde more effective than grape seed extract, not than D-002. whereas regarding to displayed benefits overwas each monotherapy regarding to plasma oxidative variables Key words: d-002, grape seed extract, lipid peroxidation, protein oxidation 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-008 Protective effect of D-002, a mixture of beeswax alcohols, against indomethacininduced gastric ulcers and mechanism of action Pérez Guerra, Yohani; Oyarzábal Year, Ambar; Mas Ferreiro, Rosa María; Molina Cuevas, Vivian; Jiménez Despaigne, Sonia Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] D-002, a mixture of higher aliphatic beeswax alcohols, produces gastroprotective and antioxidant effects. To investigate theofgastroprotective of D-002 against indomethacin-induced oxidative variables D-002, a mixture higher aliphaticeffect beeswax alcohols, produces gastroprotective ulcers, and antioxidant effects. and myeloperoxidase (MPO) activity in the gastric mucosa were examined. Rats wereoxidative randomized into To investigate the gastroprotective effect of rat D-002 against indomethacin-induced ulcers, six groups: negative vehicle (MPO) control activity and five indomethacin (50 mg/kg) groups, a variables anda myeloperoxidase in the rat gastric mucosa weretreated examined. Ratscomprising were positive control, three groups treated vehicle orally with D-002 (5, indomethacin 25 and 100 mg/kg) andtreated one group with randomized into six groups: a negative control and five (50 mg/kg) groups, omeprazole mg/kgcontrol, intraperitoneally (ip). The contents malondialdehyde (MDA), carbonyl comprising a 20 positive three groups treated orally withofD-002 (5, 25 and 100 mg/kg)protein and one group groups (PCG), hydroxyl generation glutathione peroxidase (GSH- PX), with omeprazole 20 mg/kgradical intraperitoneally (ip).and Thecatalase contents (CAT), of malondialdehyde (MDA), protein superoxide dismutase and MPOgeneration enzyme activities in the rat gastric mucosa were assessed. carbonyl groups (PCG), (SOD) hydroxyl radical and catalase (CAT), glutathione peroxidase (GSHIndomethacin increased the(SOD) content MDAenzyme and PCG, the generation of *OH mucosa radical and enzyme PX), superoxide dismutase andofMPO activities in the rat gastric wereMPO assessed. activity, while itincreased decreased CAT, GSH-PX andPCG, SOD the activities as compared to the and negative Indomethacin thethe content of MDA and generation of *OH radical MPO controls. enzyme D002 (5-100 mg/kg) significantly and GSH-PX dose-dependently ulceration to 75D%. activity, while it decreased the CAT, and SOD reduced activities indomethacin-induced as compared to the negative controls. Also, D-002 mg/kg) decreased the content MDA and PCG, the generation of hydroxyl radicals and MPO activity 002 (5-100 significantly and of dose-dependently reduced indomethacin-induced ulceration to 75 %. as compared to the positive controls. dosethe of generation D-002 (100ofmg/kg) increased GSHAlso, D-002 decreased the content of The MDAhighest and PCG, hydroxyl radicals significantly and MPO activity PX and SOD activities, whilecontrols. all dosesThe used increased activities. 20 mg/kg, the referenc as compared to the positive highest doseCAT of D-002 (100 Omeprazole mg/kg) increased significantly GSH-e drug, reduced significantly theallulcers %),increased MDA andCAT PCG, the generation of hydroxyl radicals PX and SOD activities, while doses(93 used activities. Omeprazole 20 mg/kg, the and MPO activity, increased CAT, GSH-PX and SOD activities. D-002 treatment produced gastroprotect ive referenceand drug, reducedthe significantly the ulcers (93 %), MDA and PCG, the generation of hydroxyl radicals effects against indomethacin-induced gastric ulceration, whichactivities. can be related to the reduction of hydroxyl and MPO activity, and increased the CAT, GSH-PX and SOD D-002 treatment produced radical generation, lipidagainst peroxidation, protein oxidation and ulceration, MPO activity, increase gastroprotective effects indomethacin-induced gastric whichand cantobethe related to theof the antioxidant activities in the rat gastric mucosa. reduction o enzymes and Key words: Protective effect, D-002, beeswax alcohols, indomethacin- induced, gastric ulcers, mechanism of action 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-009 Effects of D-002 on models of acute inflammation Carbajal Quintana, Daisy; Molina Cuevas, Vivian; Mas Ferreiro, Rosa María; Ravelo Calzado, Yazmin; Arruzazabala Valmaña, Lourdes Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] Oral treatment with D-002, a mixture of 6 high molecular weight aliphatic primary alcohols purified from beeswax, has been shown atomixture produceof anti-inflammatory effects aliphatic in the carrageenan-induced pleurisy Oral treatment with D-002, 6 high molecular weight primary alcohols purified fromand in the cotton models, but its effects on other models acute inflammation have not been beeswax, haspellet beengranuloma shown to produce anti-inflammatory effects in theofcarrageenan-induced pleurisy and investigated. This study was aimed to but investigate theonanti-inflammatory effectsinflammation of single oralhave doses in the cotton pellet granuloma models, its effects other models of acute notof D002 dextran-, histaminerat paw oedema models, effects and onofthe xylenebeenon investigated. This studyand wasserotonin-induced aimed to investigate the anti-inflammatory single oral induced doses ear oedema in mice. histamineOral pre-treatment with D-002 (200, 400oedema and 800 mg/kg) inhibited of D-002 on dextran-, and serotonin-induced rat paw models, andsignificantly on the xylenedextran-induced paw in oedema (p< 0.001) up to 71.4%. D-002 (200, (800 mg/kg), or 400 mg/kg, inhibited 400 andnot 800200 mg/kg) significantly induced ear oedema mice. Oral pre-treatment with D-002 significantly histamine - and serotonin-induced hinduppaw oedemas in rats 57 (p <not 0.01) 36% (p < inhibited dextran-induced paw oedema (p< 0.001) to 71.4%. D-002 (800bymg/kg), 200and or 400 0.001), respectively, while diphenhydramine (60 mg/kg) and serotonin mg/kg)inproduced mg/kg, inhibited significantly histamine - and serotonin-induced hind paw (10 oedemas rats by 57significant (p < 0.01) inhibitions (p <respectively, 0.05) and 79% (p diphenhydramine < 0.01), respectively. Also, D-002 (200 and(10 400mg/kg) mg/kg), not 50 and 36% (pof<60% 0.001), while (60 mg/kg) and serotonin produced mg/kg, significantly dose-dependently 0.951, 0.05)respectively. reduced by 50 %a xyleneinduced oedema significant inhibitionsand of 60% (p < 0.05) and(r= 79% (p < p0.01), Also, D-002 (200 and 400 formation mice ear. significantly Thus, this study reveals that oral treatment single dosesby of 50 D-002 exhibited mg/kg), notin50 mg/kg, and dose-dependently p 0.05) reduced % a xylene(r= 0.951, with significant anti-inflammatory in experimental models of acute inflammation. induced oedema formation inactivity mice ear. Thus, this study reveals that oral treatment with single doses of Key words: D-002, acute inflammation 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-010 Effects of D-002 on aspirin induced ulcers and neutrophil infiltration on the gastric mucosa Valle Clara, Maikel; Molina Cuevas, Vivian; Mas Ferreiro, Rosa María; Noa Puig, Miriam; Mendoza Castaño, Sarahí; Oyarzábal Year, Ambar; Mendoza Hernández, Nilda Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] The gastric mucosa mucosaisissusceptible susceptibletoto effects of aggressive factors, which are counterbalanced by The gastric thethe effects of aggressive factors, which are counterbalanced by mucosal defensive factors. Acid peptic diseases result from the imbalance between these aggressive and mucosal defensive factors. Acid peptic diseases result from the imbalance between these aggressive and defensive factors. Aspirin-induced Aspirin-inducedulcer ulceris ais model a model of NSAIDs-induced damage in neutrophil which neutrophil defensive factors. of NSAIDs-induced damage in which infiltration infiltration play play a a key key role. role. D-002, D-002, aa mixture mixture of of 66 higher higher aliphatic aliphatic alcohols, alcohols, has has been been shown shown gastroprotect ive and anti-inflammatory effects. This workeffects. investigates the protective effect D-002 against aspirin-induc ed gastroprotective and anti-inflammatory This work investigates the of protective effect of D-002 ulcers associated neutrophil infiltration in the gastric mucosa. were randomized sixwere groups of againstand aspirin-induced ulcers and associated neutrophil infiltrationRats in the gastric mucosa.into Rats 8 rats each. A negative vehicle and A five aspirin vehicle (300 mg/kg)-treated groups: a positive control, orally randomized into six groups of 8control, rats each. negative control, and five aspirin (300 mg/kg)-treated treated the vehicle, D-002 (25, and 100 mg/kg, respectively) with 10 mg/kg groups: with a positive control,three orallywith treated with the50 vehicle, three with D-002 (25, 50 and and other 100 mg/kg, Omeprazole. Five other (5) hours after induced damage the Thedamage stomachs removed respectively) and with 10 mg/kg Omeprazole. Fiverats (5) were hourssacrificed. after induced thewere rats were and opened, lesions were examined macroscopically andlesions microscopically. indexes and sacrificed. Theand stomachs removed and opened, and examined Ulcer macroscopically andneutrophil infiltration per ulcer areas wereand measured. positive, per noneulcer negative, controls exhibitedAllaspirin-induc ed microscopically. Ulcer indexes neutrophilAllinfiltration areas were measured. positive, ulcers. Oral treatment D-002aspirin-induced (25-100 mg/kg) dose-Oral dependently and D-002 significantly reduced none negative, controls with exhibited ulcers. treatment with (25-100 mg/kg)aspirindoseinduced gastric (37 toreduced 75%), the mean numbergastric of microscopic (40 the to 72%) neutrophil dependently andlesions significantly aspirin-induced lesions (37ulcers to 75%), mean and number of infiltration (41.7 to 83.1%) in theand rat gastric mucosa. In turn, omeprazole significantly the microscopic ulcers (40 to 72%) neutrophil infiltration (41.7 to 83.1%)10 in mg/kg the rat reduced gastric mucosa. In turn, ulcer indexes, mean microscopic ulcer number neutrophil infiltration per areaulcer of microscope omeprazole 10the mg/kg reduced significantly the ulcerand indexes, the mean microscopic number andfield, respectively. Oral treatment D-002 (25- field, 100 mg/kg) effectively against aspirin-induced ulcers neutrophil infiltration per areawith of microscope respectively. Oralprotects treatment with D-002 (25- 100 mg/kg) and in the gastric mucosa induced by aspirin ulceration. effecthil infiltration in the Key words: D-002, aspirin induced ulcer, neutrophil infiltration, gastric mucosa 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-011 Effects of D-002 and Grape Seed Extract on monoiodine-acetate induced osteoarthritis in rats Mendoza Castaño, Sarahí; Noa Puig, Miriam; Mas Ferreiro, Rosa María; Valle Clara, Maikel; Mendoza Hernández, Nilda Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected]; [email protected] Osteoarthritis (OA), the most common age-related degenerative joint disease in adults, is characterized by byprogressive a a progressive cartilage cartilage loss loss which which produces producesdebilitating debilitatingchronic chronicpain painininaffecting affectingindividuals individuals and andtends tends to to worsen worsen over overtime timeasascartilage cartilagewears wearsaway. away.Non-pharmacologic Non-pharmacologic interventions interventions are are the cornerstone of of OA OA management. Nevertheless, optimal treatment should should combine combine non-pharmacological non-pharmacological and and pharmacological pharmacological modalities. Numerous modalities. studies Numerous have demonstrated studies havethat demonstrated oxidative stress that oxidative has beenstress implicated has been in OA which implicated support theinevaluation OA whichofsupport antioxidants the evaluation for management of antioxidants of OA. D-002 for management is a mixtureof of OA. six high D-002 molecular is a mixture weight of six highalcohols aliphatic molecular purified weightfrom aliphatic beeswax alcohols that purified has been from shown beeswax to bethat effective has been in experimental shown to be effective models of in experimental acute and chronic models inflammation of acute and chronic in experimental inflammation models andofinOA. experimental The aim ofmodels this study of OA. wasThe to compare aim of this study effects of was D-002 to and compare Grapeeffects seed of extract D-002(GSE) and Grape in mono seed iodoacetate extract (GSE) induce in mono OA iniodoacetate rats. Male wistar inducerats OA indistributed were rats. Male into wistar 6 groups: rats were one distributed negative into control 6 groups: and fiveone groups negative with control MIA induce and five OA:groups a positive withcontrol MIA induce and group OA: four a positive groupscontrol treated group withand D-002 four (200 groups andtreated 400 mg/kg) with D-002 or GSE (200(200 and and 400 400 mg/kg) mg/kg). or GSE OA(200 was and 400 mg/kg). induced by MIA. OA Substances was induced wereby administered MIA. Substances during were 10 administered days. Cartilage during changes 10 days. were Cartilage measured by changes using histological were measured and Mankin by usingmodified histological score. and D-002 Mankin(200 modified and score. 400 mg/kg) D-002 (200 and and GSE400 (400 mg/kg) mg/kg) and GSE (400 significantly prevented mg/kg) significantly the increaseprevented on total histological the increase score on total induced histological by the injection score induced of MIA by intothe the knee injection joint of the of MIA animals. into the Effects knee of joint D-002 of thewere animals. significantly Effects of higher D-002than wereGSE significantly ones in higher the reduction than GSE of total histological score. D-002 (200 and 400 score. mg/kg)D-002 significantly reduce histological parameters of the score ones in the reduction of total histological (200 and 400 mg/kg) significantly reduce histological except cellular parameters abnormalities of theand score osteoclasts except cellular presences. abnormalities On its side, andGSE osteoclasts was no effective presences. on On extension its side,to GSE was cellular damage, no effective abnormalities, on extension bone to loss damage, and panus cellularformation abnormalities, and osteoclast bone losspresence. and panus D-002 formation was more and effective to GSE in preventing cartilage damage in MIA- induced OA model in rats. Key words: osteoarthritis, d-002, mono iodoacetate, rats 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-012 Effects of D-002, a mixture of high molecular weight beeswax alcohols, on patients with non- alcoholic fatty liver disease (NAFLD) Illnait Ferrer, José; Rodríguez, Iván; Mendoza Castaño, Sarahí; González, Yolanda; Mas Ferreiro, Rosa María; Miranda, Migdalia; Fernández Travieso, Julio César; Mesa, Meilis; Fernández Dorta, Lilia de la C.; Carbajal D., Daisy; Gámez Menéndez, Rafael Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] BACKGROUND. Non-alcoholic fatty liver disease (NAFLD), characterized by excessive liver fat accumulation without habit of alcohol consumption, is intimately related tobyinsulin resistance BACKGROUND. Non-alcoholic fatty liver disease (NAFLD), characterized excessive liver fatand ranges from a benign without course to liveroffibrosis cirrhosis. NAFLD management mainly involves dietary modification accumulation habit alcoholand consumption, is intimately related to insulin resistance and ranges and loss. Although no fully successful pharmacological intervention mainly is available, alternative fromweight a benign course to liver fibrosis and cirrhosis. NAFLD management involves dietary therapies to treat NAFLD promisingnoresults. D-002, apharmacological mixture of beeswax alcohols with antioxidant modification and have weightshown loss. Although fully successful intervention is available, effects, hastherapies been shown hepatoprotective TheD-002, effectsaofmixture D-002 of onbeeswax NALFD, however, alternative to treat NAFLD have effects shown experimentally. promising results. alcohols had not been investigated. investigate the efficacyeffects and safety of D-002 on NALFD. with antioxidant effects, has AIM: beenTo shown hepatoprotective experimentally. Thepatients effects with of D-002 on METHODS: This placebo-controlled study included 50 patients with NAFLD randomized to placebo (n = NALFD, however, had not been investigated. 25) 50 mg the (n =efficacy 25) tablets twice aofday for 24 endpoint was to obtain a significant AIM:orToD-002 investigate and safety D-002 on weeks. patientsPrimary with NALFD. METHODS: reduction of This ultrasonography-detected placebo-controlled study liverincluded fat infiltration 50 patients as compared with NAFLD to placebo. randomized Secondary to placebo endpoints (n = were obtain50decreases of tablets the Human (HOMA) index,was insulin levels,a serum liver 25) ortoD-002 mg (n = 25) twiceOmeostatic a day for 24Assesment weeks. Primary endpoint to obtain significant enzymes, increase of plasma total antioxidant status (TAS) and improvement clinical symptoms reduction of ultrasonography-detected liver fat infiltration as compared to placebo. of Secondary endpoints as compared to placebo. RESULTS: At randomization, all indicators(HOMA) were comparable in both groups. were to obtain decreases of the Human Omeostatic Assesment index, insulin levels, serumSeven liver enzymes,D-002(28.0%) increase patients of plasma and none total antioxidant placebo exhibited status (TAS) a normal andliver improvement echopattern of clinical ultrasonography symptoms(p<0.01) as at study completion. compared to placebo. Also, D-002 reduced significantly (p<0.01 as compared to baseline and placebo) HOMA indexAtand insulin levels,allincreased unaffectedinthe remaining parameters. TheD-002frequency of RESULTS: randomization, indicatorsTAS wereand comparable both groups. Seven (28.0%) D002 patients (12/25, 48.0%) referring symptom higher (p<0.001) than in patients and none placebo exhibited a normal liverimprovement echopattern was ultrasonography (p<0.01) at placebo study (1/25, 4.0%). Treatment was safe and well tolerated.(p<0.01 Six patients, three ofto each group,and withdrew from the study, completion. Also, D-002 reduced significantly as compared baseline placebo) HOMA only because of adverse (100 mg/day) was effective indextwo and(placebo) insulin levels, increased TAS experiences. and unaffectedCONCLUSION: the remaining D-002 parameters. The frequency of D- to improve ultrasonographic insulin resistance (HOMA index, than insulin levels), (1/25, plasma 002 patients (12/25, 48.0%)findings, referringindicators symptom of improvement was higher (p<0.001) in placebo TAS and clinical evolution thiswell population of Six patients with three NAFLD. Further studies, however, 4.0%). Treatment was safeonand tolerated. patients, of each group, withdrew from are the needed study, only to confirm two (placebo) these results. because of adverse experiences. CONCLUSION: D-002 (100 mg/day) was effective to improve ultrasonographic findings, indicators of insulin resistation of Key words: D-002, beeswax alcohols, patients non- alcoholic fatty liver disease, NAFLD 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-013 Effects of D-002 (beeswax alcohols) on gastrointestinal symptoms and oxidative markers in middle-aged and older patients Fernández Dorta, Lilia de la C. ; Rodríguez, Iván; Molina Cuevas, Vivian; Mendoza Castaño, Sarahí; Mas Ferreiro, Rosa María; Oyarzábal Year, Ambar; Pérez Guerra, Yohani; Mesa, Meilis; Fernández Travieso, Julio César; Gámez Menéndez, Rafael; Jiménez Despaigne, Sonia; Ruiz, Dalmer; Cruz, Yadira Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] This study investigated the persistence and coexistence of the effects of D-002, a mixture of higher aliphatic beeswax with antioxidant and gastroprotective symptoms andaplasma This studyalcohols investigated the persistence and coexistenceeffects, of the on effects of D-002, mixtureoxidative of highermarkers in subjects with gastrointestinal complaints. and Sixty subjects were effects, double-blinded randomised to D-002 (50 aliphatic beeswax alcohols with antioxidant gastroprotective on symptoms and plasma mg) or placebo twicewith dailygastrointestinal for 24 weeks.complaints. Dose was Sixty titrated to three tablets daily if symptoms, oxidative markerstablets in subjects subjects were double-blinded assessed the Gastrointestinal Symptom Rating (GSRS), did Dose not improve after to6 three weeks. No randomisedwith to D-002 (50 mg) or placebo tablets twice Scale daily for 24 weeks. was titrated significant changes occurred in placebo. D-002 persistently reduced (p < 0.001) GSRS overall score and tablets if symptoms, the Gastrointestinal did were not less several daily sub-scores versusassessed placebo. with Dose-titration (1/30) andSymptom antacids Rating intake Scale (7/30) (GSRS), with D-002 improve after 6 weeks. No significant changes occurred in placebo. D-002 persistently reduced (p < (p < frequent (p < 0.001) than with placebo (29/30 and 28/30, respectively). D-=02 persistently decreased 0.001) GSRS score and(pseveral sub-scores versus placebo. and Dose-titration antacids 0.00001) total overall hydroperoxides, < 0.001) plasma malondialdehyde sulphydril (1/30) groups,and while increased intake (7/30) with D-002 were less frequent (p < 0.001) than with (100 placebo (29/30administered and 28/30, respectively). (p < 0.01) plasma total antioxidant status. Summarizing, D-002 mg/day) for 24 weeks D-=02 persistently displayed persistentdecreased antioxidant (p < and 0.00001) gastroprotective total hydroperoxides, effects in subjects (p < 0.001) with plasma gastrointestinal malondialdehyde complaints. OBJECTIVES: In light of these issues, this persistence and coexistence ofD-002 the effects and sulphydril groups, while increased (p <study 0.01)investigated plasma totalthe antioxidant status. Summarizing, of D-002, given for 6 months, symptoms andpersistent plasma oxidative markers of middle-agedeffects and older (100 mg/day) administered for on 24 the weeks displayed antioxidant and gastroprotective in subjects with habitual gastrointestinal complaints. CONCLUSIONS This study demonstrates that oral subjects with gastrointestinal complaints. treatment with D-002 (100 mg/day) for 24 weeks ameliorated symptoms and improved plasma oxidative markers in middle-aged and older subjects with habitual gastrointestinal complaints. These results support OBJECTIVES: In light of these issues, this study investigated the persistence and coexistence of the the coexistence and persistence of symptom relief and antioxidant effects of D-002 and that its antioxidant effects of D-002, given for 6 months, on the symptoms and plasma markers of middle-aged and effect could contribute, at least partially, to its gastroprotective effectoxidative in such population. older subjects with habitual gastrointestinal complaints. Key words: D-00, beeswax alcohols,gastrointestinal symptom, oxidative marker, middle-aged CONCLUSIONS older patient This study demonstrates that oral treatment with D-002 (100 mg/day) for 24 weeks ameliorated symptoms and improved plasma oxidative markers in middle-aged and older subjects with habitual gastrointestinal complaints. These results support the coexistence and persistence of symptom relief and 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-014 Three years of long term stability of 5 mg Prevenox tablets in blisters Sierra Pérez, Roxana de la Caridad; González Canavacciolo, Víctor L.; Vicente Murillo, Roxana; Rodríguez Leyes, Eduardo Antonio; Marrero Delange, David Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] El prevenox constituye constituyeuna una mezcla mezclareproducible reproducible de deácidos ácidosgrasos grasosdede2424a 36 a 36 átomos átomos dede carbono carbono purificados purificados de la cera de delalacaña cera de de azúcar. la caña Esta de azúcar. sustancia, Estacon sustancia, efectos con antioxidantes, efectos antioxidantes, es empleadaesen empleada la elaboración en la elaboración de tabletas 5 mg de ácidos, forma terminada utilizada enclínicos los ensayos en la de tabletas con 5 mg de con ácidos, forma terminada utilizada en los ensayos y en laclínicos prácticay de rutina. práctica de fue rutina. El objetivo fue determinar la estabilidad de blísteres estas tabletas en blísteres de cloruro de El objetivo determinar la estabilidad de estas tabletas en de cloruro de polivinilo y aluminio. polivinilo yde aluminio. Muestras de tres lotes de tabletas (MedSol,enCuba) en mantuvieron cajas de cartón y Muestras tres lotes de tabletas (MedSol, Cuba) se pusieron cajas se depusieron cartón y se en las se mantuvieron las condiciones la zona climática 2 C, 70 relativa) 5 % de humedad relativa) condiciones de en la zona climática IVde(30 2 C, 70 5 IV % (30 de humedad durante tres años. Los durante tres evaluados años. Los se parámetros evaluados se sus mantuvieron dentro de especificaciones parámetros mantuvieron dentro de especificaciones desus calidad durante todode el calidad estudio: durante todo apariencia (tabletas el estudio: verdes apariencia con superficies (tabletas enteras), verdes con peso superficies promedioenteras), (inicial peso 10 %),promedio contenido (inicial total de10los %), contenido ácidos grasos total (5 ±de0,5 losmg), ácidos tiempo grasos de (5desintegración ± 0,5 mg), tiempo (< 30 de min), desintegración y contenido (< 30 microbiológico min), y contenido (? 1000 microbiológico bacterias/g y ? (? 1001000 hongos/g, bacterias/g ausencia y ? 100 de E. hongos/g, coli, S. aureus, ausencia Pseudomonas, de E. coli, S.Salmonella aureus, Pseudomonas, y C. albicans). Los Salmonella del resultados estudio Los resultados sustentandel quepresente las tabletas de sustentan prevenox con de 5mg, y C.presente albicans). estudio que dosis las tabletas dealmacenadas prevenox conlas en dosis condiciones de 5mg, almacenadas de la zona climática en las condiciones IV, presentandeunlatiempo zona climática de vida útil IV,de presentan tres años. un tiempo de vida Key words: prevenox, cera de caña de azúcar, ácidos grasos, estabilidad, tabletas 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-015 Effects of D-003 in the vertebrae and lipid profile in a model of osteoporosis induced by glucocorticoid and ovariectomy in rabbits Valle Clara, Maikel; Noa Puig, Miriam; Mendoza Castaño, Sarahí; Pérez Guerra, Yohani; Oyarzábal Year, Ambar; Nodal Flores, Carlos Ramón; Mendoza Hernández, Nilda; Jiménez Despaigne, Sonia Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] Osteoporosis is a chronic degenerative systemic disease characterized by microarchitectural disrepair of bone tissue accompanied the progressive reduction of bone mass. D-003 is a mixture of verydisrepair long- chain Osteoporosis is a chronic by degenerative systemic disease characterized by microarchitectural of aliphatic acids purified from sugarcane which has shown cholesterol-lowering effects in experimental bone tissue accompanied by the progressive reduction of bone mass. D-003 is a mixture of very long- and clinical studies.acids Previous studies rats havewhich shownhas that D-003 prevent bone loss effects and decreased bone chain aliphatic purified from in sugarcane shown cholesterol-lowering in resorption in ovariectomized rats. Previous This studystudies was undertaken investigate the effects of D-003 a model experimental and clinical studies. in rats havetoshown that D-003 prevent bone in loss and of osteoporosis by in ovariectomy and glucocorticoids in female rabbits. the Female New decreased boneinduced resorption ovariectomized rats. This studyadministration was undertaken to investigate effects of Zealand white rabbits skeletally matured were used in 4 groups. A sham group of false operated rats D-003 in a model of osteoporosis induced by ovariectomy and glucocorticoids administration in female and three ovariectomized treated withmatured prednisolone animals; a positive control rabbits.groups FemaleofNew Zealand white and rabbits skeletally were used in 4 groups. A sham group group of administered and twoofgroups treated with with D-003 (50 and 200 mg / false operatedwith ratsthe andvehicle three groups ovariectomized andD-003. treatedTreatment with prednisolone animals; a positive kg) significantly prevented with decreased trabecular numbertreated and thickness while decreased trabecular control group administered the vehicle and two groups with D-003. Treatment with D-003 (50 separation, of osteoclast and osteoclast increased thethickness induced damage. In line with and 200 mg number / kg) significantly prevented decreasedsurface trabecular numberbyand while decreased these results, D-003 number significantly preventedand theosteoclast increase surface in Totalincreased cholesterol andinduced TG at damage. both doses trabecular separation, of osteoclast by the In improving the lipid profile of animals treated with D-003, allowing for the first time linking lipid lowering line with these results, D-003 significantly prevented the increase in Total cholesterol and TG at botheffect antiresorptive effect. doses improving the lipid profile of animals treated with D-003, allowing for the first time linking lipid Key words: d-003, glucocorticoids, osteoporosis, rabbit 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-016 Effect of D-003 and soy standardized extract rich in daidzein on osteoporosis induced in ovariectomized rats Noa Puig, Miriam; Mendoza Castaño, Sarahí; Valle Clara, Maikel; Mendoza Hernández, Nilda; Mas Ferreiro, Rosa María Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] D-003 is a mixture of higher fatty acids purified from sugarcane wax with antiosteoporotic effects in ovariectomized (ovx) rats. Soy standardized extract rich in daidzein (SSE-D) have shown bone-protect ive effects. This study compared the effect of D-003 and SSE-D on bones of ovx rats. Rats were randomized into 6 groups: one false-operated and five ovx groups: a positive control treated orally with the vehicle, two with D-003 (50 and 200 mg/kg) and two with SSE-D (15 and 30 mg/kg) for 3 months. Ovx decreased trabecular volume, number and thickness of trabeculae in the fifth vertebrae and femoral neck, and increased trabecular separation, osteclast number and surface versus the false-operated group. D-003 and SSE-D prevented all changes induced by ovx, but the effects of D-003 were significantly greater than those of SSE-D. Concluding, D-003 (50 and 200 mg/kg) and SSE-D (15 and 30 mg/kg) prevented osteoporotic changes in ovx rats, but D-003 was more effective than the SSE-D preparation assayed. Key words: D-003, soy standardized extract, daidzein, osteoporosis, ovariectomized rat 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-017 Effects of D-003 on quality of life in postmenopausal women at moderate to high risk of osteoporosis. Fernández Travieso, Julio César; Ceballos, Alfredo; Illnait Ferrer, José; Mendoza Castaño, Sarahí; Fernández Dorta, Lilia de la C.; Mas Ferreiro, Rosa María; Gámez Menéndez, Rafael; Mesa, Meilis; Cruz, Yadira Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] Osteoporosis, a disease characterized by reduction of bone mineral density (BMD) and alteration of bone microarchitecture that increases bone fragility and fracture affects the quality of lifealteration of the sufferers Osteoporosis, a disease characterized by reduction of bone risk, mineral density (BMD) and of bone. Pharmacological osteoporosis of drugs that of reduce resorption microarchitecture management that increases of bone fragility andincludes fracture the risk,use affects the quality life ofbone the sufferers. and/or increase bone synthesis. is a mixture of high molecular weight aliphaticbone acids purified from Pharmacological management ofD-003 osteoporosis includes the use of drugs that reduce resorption sugar waxbone withsynthesis. antiresorptive demonstrated in experimental and clinical studies.from This and/or cane increase D-003 effects is a mixture of high molecular weight aliphatic acids purified randomized, double-blinded, placebo-controlled study investigated the effects of D-003studies. on the This quality of life sugar cane wax with antiresorptive effects demonstrated in experimental and clinical of postmenopausal women at placebo-controlled moderate to high risk of OP. Forty postmenopausal were randomized, double-blinded, study investigated the effects of women D-003 on the randomized quality of to placebo or D-003 (10atmg/d) for three months. on Qualeffo total score and lifereceive of postmenopausal women moderate to high risk ofSignificant OP. Forty reductions postmenopausal women were serum low-density lipoprotein-cholesterol (LDL-C) levels versus placebo groupreductions were considered as the randomized to receive placebo or D-003 (10 mg/d) for three months. Significant on Qualeffo total score primary andand secondary serum low-density efficacy variables, lipoprotein-cholesterol respectively. Evaluation of safety tolerability (LDL-C) levels versusand placebo groupincluded were the considered analyses of as thethe effects primary on physical and secondary and blood efficacy indicators, variables, and the respectively. adverse events (AE) reports, well. Data Evaluation of safetyasand tolerability were analyzed included as per thethe analyses Intention-to-Treat of the effects method. on physical Both groups and blood displayed indicators, similarand baseline the adverse characteristics. events (AE) reports, After completeasthree well. months Data were on therapy, analyzedD-003 as persignificantly the Intention-to-Treat decreased method. Qualeffo Both total groups scores displayed (p<0.0001 vs similar baseline baseline, p<0.05characteristics. vs placebo), asAfter well as complete LDL-C values three months (17.1 %). on The therapy, treatment D-003was significantly safe and decreased well tolerated. Qualeffo Only one total patient scores (D-003-treated) (p<0.0001 vs withdrew baseline, from p<0.05 the study, vs placebo), and it was as due welltoasprotocol LDL-C violation. values (17.1 Four%). patients, The all from placebo group, reported someOnly AE.one In conclusion, D- 003 (10withdrew mg/d) administered during treatment was safe and well tolerated. patient (D-003-treated) from the study, and three it months quality of life in postmenopausal moderate to high risk of In OP and produced was dueimproved to protocol violation. Four patients, all from women placebo at group, reported some AE. conclusion, Dadditional benefit on serum LDL-C 003 (10 mg/d) administered during values. three months improved quality of life in postmenopausal women at Key words: D-003, postmenopausal women, osteoporosis 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-018 Effects of D-003, a mixture of sugarcane wax acids, on bone marrow lesions, subchondral bone and cartilage damage in monoiodoacetate-induced osteoarthritis Noa Puig, Miriam Mendoza Castaño, Sarahí; Mas Ferreiro, Rosa María; Valle Clara, Maikel; Mendoza Hernández, Nilda Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] Osteoarthritis (OA) has been linked with bone marrow edema (BME) pattern, identified by magnetic resonance imaging and been histopathology, a mix of(BME) different pathologies, so by that BME term has Osteoarthritis (OA) has linked withwhich bone involves marrow edema pattern, identified magnetic been progressively replaced by that ofwhich bone involves marrow alesions (BML). BML is the first signBME of OA resonance imaging and histopathology, mix of different pathologies, so that termafter experimental ligament damage, cartilage erosion and(BML). degeneration in experimentally-induc has been progressively replaced precedes by that of bone marrow lesions BML is the first sign of OA after ed OA, strongly correlate knee precedes pain incidence anderosion progressing in humans, and the reduction of BML size experimental ligament with damage, cartilage and degeneration in experimentally-induced is accompanied by pain improvement. Some studies have revealed positive and findings with bisphophonat OA, strongly correlate with knee pain incidence and progressing in humans, the reduction of BML es and impact on the progression of cartilage Oral revealed administration of findings D-003, awith mixture of high size their is accompanied by pain improvement. Some defects. studies have positive molecular weightand sugar wax acids, has beenof shown produceOral bone antiresorptive effectsa in bisphophonates their cane impact on the progression cartilagetodefects. administration of D-003, experimental clinical studies. This study investigated thebeen effects of D-003 on BML, subchondral bone mixture of highand molecular weight sugar cane wax acids, has shown to produce bone antiresorptive and cartilage by sodiumand monoiodoacetate OA in rats.the Animals distributed into 5 groups: effects in experimental clinical studies.(MIA)-induced This study investigated effectswere of D-003 on BML, a negative control injected with MIA: one positive control and three with (100, 200were and 400 subchondral bone and four cartilage by sodium monoiodoacetate (MIA)-induced OA inD-003 rats. Animals mg/kg). Theinto damage wasa induced a single of MIA theone synovial of and the knee, after distributed 5 groups: negative by control three with control and injection four injected withinto MIA: positivecavity which gastricThe gavage for 10 Kneebyjoint were injection removedofand for light D-003 rats (100,were 200 treated and 400by mg/kg). damage wasdays. induced a single MIAprocessed into the synovial microscopy. Slidesafter werewhich stained and eosin and for toluidine blue to stain proteoglycan cavity of the knee, ratswith werehematoxylin treated by gastric gavage 10 days. Knee joint were removed for analyze the cartilage. Mankin score was were usedstained to assess depth andand extent of and cartilage damage. and processed for lightAmicroscopy. Slides withthe hematoxylin eosin toluidine blue toNo negative, but all for positive control animals,A exhibited BMLs, D-003 decreased stain proteoglycan analyze the cartilage. Mankin score was meanwhile used to assess the treatment depth and extent of significantly the frequency of animals with BMLs. Negative controls did BMLs, not show tibial plateau cartilage damage. No negative, but all positive control animals, exhibited meanwhile D-003 extent of damage, loss or presence the of osteoclasts, thesewith variables significantly increased treatment bone decreased significantly frequency of all animals BMLs. were Negative controls did not showin the positive control group. Oral D-003 ameliorated the damage to the subchondral lowering tibial plateau extent of damage, bone loss or presence of osteoclasts, all these bone, variables were the extent of tibial damage, bone loss, and the presence of osteclasts in the knee joint, compared significantly increased in the positive control group. Oral D-003 ameliorated theas damage to thewith positive controls. Oralbone, treatment withtheD-003 effective for reducing subchondral and cartilage subchondral lowering extentwas of tibial damage, bone loss,BMLs, and the presence ofbone osteclasts in the damage, OApositive in the rat knee, Oral which suggests it may components of knee joint,inasMIA-induced compared with controls. treatment withthat D-003 wasbenefit effectivemore for reducing BMests that itjoint. osteoarthitic Key words: D-003, sugarcane wax acids, bone marrow lesions, bone and cartilage damage, osteoarthritis 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-019 Stability of D-004 active ingredient in amber glass and high density polyethylene flasks Rodríguez Leyes, Eduardo Antonio; González Canavacciolo, Víctor L.; Sierra Pérez, Roxana de la Caridad; Vicente Murillo, Roxana; Morales Rico, Carmen Luisa; Marrero Delange, David Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] farmacéutico activo activo obtenido obtenido de de los los frutos frutos de de la lapalma palma real real cubana cubana (Roystonea El D-004 es un ingrediente farmacéutico (Roystonea regia), compuesto fundamentalmente por una ácidos grasos libres 8 y 18 de regia), compuesto fundamentalmente por una mezcla de mezcla ácidos de grasos libres entre 8 y entre 18 átomos átomos decon carbono, carbono, potencialidades con potencialidades en el tratamiento en el tratamiento de la hiperplasia de la hiperplasia prostáticaprostática benigna. benigna. Para determinar Para la determinar vida útil del la D-004 vida útil en frascos del D-004 de en vidrio frascos ámbar de yvidrio de polietileno ámbar y de polietileno alta densidad, de alta en las densidad, zonas climáticas en las IV yzonas II, muestras climáticas de IV tres y II, lotes muestras pilotos de se tres colocaron lotes pilotos a 30 ºC/70 se colocaron % HR y a2530ºC/ ºC/70 60 % % HR, HR se y 25determinaron ºC/ 60 % HR,las se determinaron organolépticas características las características y organolépticas los contenidosy los decontenidos material deinsaponificable, material insaponificable, ácidos grasos ácidos y grasos y microbiológicos microbiológicos desde el tiempo desde el inicial tiempo hasta inicial los 51 hasta meses, los 51 y se meses, calcularon y se calcularon los tiemposlos de tiempos vida útil mediante de vida útil mediante análisis En estadísticos. En vidrio ámbar, en ambas zonas el D-004 sus análisis estadísticos. vidrio ámbar, en ambas zonas el D-004 mantuvo sus mantuvo especificaciones de calidad especificaciones de calidad por más de del 24 meses. La de disminución del contenido por más de 24 meses. La disminución contenido ácidos, ocurrida primerodeenácidos, la zonaocurrida IV, permitió primero en los la zona IV, permitió nuevos tiempos útil para en ambas zonas. En el D-004 establecer nuevos tiempos establecer de vida útillos para ambas zonas.deEnvida polietileno, ambas zonas, polietileno, ambas zonas, el D-004 cumplió parámetros evaluados durante los primeros 30 de cumplió conenlos parámetros evaluados durantecon loslosprimeros 30 meses, posteriormente el contenido meses,disminuyó posteriormente contenido de ácidos al límite establecido ácidos hastaelresultar inferior al límitedisminuyó establecido hasta en resultar varias deinferior las muestras. Se demostróenque varias de las muestras. Se demostró que el ingrediente activo D004, obtenido a escala pilotoun y envasado el ingrediente activo D004, obtenido a escala piloto y envasado en vidrio ámbar, presenta tiempo de en ámbar, presenta un tiempo útil en superior a losclimáticas 24 meses IV (33y yII,48 meses en las zonas vidavidrio útil superior a los 24 meses (33 yde 48vida meses las zonas respectivamente), mientras climáticas al ser envasado IV y II,en respectivamente), frascos de polietileno mientras de alta al ser densidad envasado presenta en frascos tiempos de polietileno de vida útilde dealta 30 ydensidad 33 meses en las zonas climáticas IV y II, respectivamente. Key words: d-004, estabilidad, vidrio ámbar, polietileno de alta densidad, roystonea regia 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-020 Preliminary stability of soft gelatin capsules with D-004 active ingredient Rodríguez Leyes, Eduardo Antonio; González Canavacciolo, Víctor L.; Sierra Pérez, Roxana de la Caridad; Vicente Murillo, Roxana; Morales Rico, Carmen Luisa; Marrero Delange, David Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] El desarrollo de de cápsulas cápsulas blandas blandasde deD004, D004,ingrediente ingredienteactivo activocon con potencialidades potencialidades en en la prevención la prevención y y tratamiento de delalahiperplasia hiperplasiaprostática prostática benigna, benigna, conllevó conllevó al estudio al estudio de estabilidad de estabilidad de esta de forma esta forma loteslotes pilotopiloto de cápsulas con 320con mg 320 de ácidos totales (LIPA farmacéutica. Se Seemplearon emplearontrestres de cápsulas mg de ácidos totales (LIPA PHARMACEUTICALS, Australia) ámbarámbar de tereftalato de polietileno (40 Australia)envasadas envasadasen frascos en frascos de tereftalato de polietileno (40 cápsulas/frasco). El estudio de estabilidad acelerada se realizó a 40 ºC ºC yy 75 % % HR HR yy los los estudios estudios aa largo largo %% HR) y IV (30(30 ºC ºC y 70 % % plazo se desarrollaron desarrollaron en en condiciones condiciones de delas laszonas zonasclimáticas climáticasII II(25 (25ºCºCy 60 y 60 HR) y IV y 70 HR). En el el tiempo tiempoinicial inicialy yenencada cada muestreo muestreo se se determinaron determinaron las las Características Características organolépticas, organolépticas, que que coincidieron con las iniciales durante durante los los seis seis primeros primeros meses meses del del estudio estudio acelerado acelerado yyen entodos todoslos losdemás demás muestreos muestreos de los estudios de los estudios a largoaplazo; largo plazo; la Variación la Variación del peso, del peso, que seque mantuvo se mantuvo en 90-en110 90-%110 del%peso del peso promedio promedio inicial,inicial, el tiempo el tiempo de Desintegración, de Desintegración, que fue que 30 fue min 30 ymin el Contenido y el Contenido microbiológico microbiológico (una (una vez al vez alque año), año), seque mantuvo se mantuvo estable.estable. También También se determinó se determinó el contenido el contenido de ácidos de grasos ácidos por grasos Cromatografía por de Cromatografía Gases, el cual se de mantuvo Gases, elencual 320se mgmantuvo ± 32 mgen (10%) 320 por mg cápsula ± 32 mg y(10%) durante portodo cápsula el estudio y durante en condiciones todo el estudio en condiciones aceleradas y en ambas aceleradas zonas climáticas. y en ambas Según zonas los resultados climáticas.del Según estudio los resultados de estabilidad del estudio acelerada, de se estabilidad prevé que estas acelerada, cápsulas se prevé pudieran que resistir estas cápsulas hasta 6 meses pudieran en resistir condiciones hasta drásticas 6 meses yenalcanzar condiciones un tiempo drásticas de vida útil y alcanzar de 3 años un tiempo en las de condiciones vida útil dede 3 años la zona en las climática condiciones IV, losderesultados la zona climática obtenidos IV, los hasta el momento largo plazo avalan un tiempo de vida útil avalan de al menos 2 años en las ambas resultados aobtenidos hasta el momento a largo plazo un tiempo de vida útilcondiciones de al menos de 2 años zonas climáticas. Key words: d-004, estabilidad, cápsulas blandas, tereftalato de polietileno, roystonea regia 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-021 Determination of degradation products of D004 Active Ingredient Vicente Murillo, Roxana; Rodríguez Leyes, Eduardo Antonio; González Canavacciolo, Víctor L.; Sierra Pérez, Roxana de la Caridad; Morales Rico, Carmen Luisa; Marrero Delange, David Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] La determinación de de los los productos productos de dedegradación degradacióneneningredientes ingredientesactivos activos(IA) (IA) y formas y formas terminadas terminadas es es esencial como parte parte de de su suconocimiento conocimientoquímico-farmacéutico químico-farmacéuticoy yununaspecto aspectoexigido exigido porpor loslos organismos organismos anterior, se implementaron métodos analíticos para identificar y reguladores. Teniendo Teniendo en encuenta cuentalo lo anterior, se implementaron métodos analíticos para identificar y cuantificar los D004. A muestras sometidas a oxidación (peróxido de de los productos productos de dedegradación degradaciónenenel el D004. A muestras sometidas a oxidación (peróxido (80 (80 °C y°C 185y °C), les determinaron las características hidrógeno), fotólisis fotólisis (luz (luzUV) UV)y termólisis y termólisis 185se°C), se les determinaron las características índice de ácido tiobarbitúrico (IATB),(IATB), porcentaje de dienos organolépticas, índice índice dedeperóxido peróxido(IP), (IP), índice de ácido tiobarbitúrico porcentaje de dienos conjugados (%DC) y compuestos volátiles (CVol), y sus resultados se compararon con el de una una muestra muestra sometidas a 110 °C solo se les los dos de D004 sin sin degradar; degradar; mientras mientrasque quea amuestras muestras sometidas a 110 °C solo se determinaron les determinaron los dos últimos parámetros. Se mayor degradación de de los los AG AG Se determinó determinó que quelalaoxidación oxidaciónfue fuelalaque queprovocó provocóla la mayor degradación insaturados, así como los mayores IP, mientras que la termólisis fue la la que que conllevó aa mayores mayores contenidos contenidos de DC, IATBdey DC, CVol. IATB En yelCVol. caso En de el la caso termólisis de la atermólisis 110 °C se a 110 pudo°Cobservar se pudo que observar el % que DC aumentó el % DC hasta aumentó las 7 h y hasta disminuyó las 7 ah ypartir disminuyó de estea tiempo, partir dese este determinó tiempo, se pordeterminó primera vez primera presencia vez la depresencia 2-hexenal, de2por la heptenal, 2-heptanona,2-heptanona, 2-hepteno, tolueno, octano, 1-pentanol y pentilfurano en el D004 2-hexenal,2-hexanona, 2-heptenal, 2-hexanona, 2-hepteno, tolueno, octano, 1-pentanol y pentilfurano degradado. Key words: d004, productos de degradación, ácidos grasos, estabilidad 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-022 One year oral Toxicity of D-004, a lipid extract from Roystonea regia fruits, in Sprague Dawley rats Gutiérrez Martínez, Ariadne; Gámez Menéndez, Rafael; Noa Puig, Miriam; Mas Ferreiro, Rosa María; Arencibia Arrebola, Daniel Francisco; Pardo, B.; Valle Clara, Maikel; Oyarzábal Year, Ambar; Curveco, Dayisell Lázara; García, H.; Goicochea, E.; Mendoza Hernández, Nilda; Jiménez Despaigne, Sonia Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] D-004, a lipid extract of royal palm (Roystonea regia) fruits that contains a reproducible mixture of fatty acids, been shown prevent and phenylephrine-induced prostate hyperplasia D-004,has a lipid extract of to royal palm testosterone (Roystonea regia) fruits that contains a reproducible mixture ofinrodents. fatty Thisstudyinvestigatedthe oral toxicityand of D-004 in rats. Rats fromprostate both sexes were randomized acids, has been shown to long-term prevent testosterone phenylephrine-induced hyperplasia into four groups (20 rats sex/group): long-term a control and treated withinD-004 (800,from 1500both or 2000 inrodents. Thisstudyinvestigatedthe oral three toxicity of D-004 rats. Rats sexesmg/kg/day, were respectively). Atfour studycompletion, were sacrificed under of orblood randomized into groups (20 rats rats sex/group): a control and threeanesthesia. treated with Determinations D-004 (800, 1500 biochemical andrespectively). hematological parameters andratsorgan weight were Also, Determinations necropsy and 2000 mg/kg/day, At studycompletion, were sacrificed underdone. anesthesia. histopathological studies performed. Four of 160 diedweight before were studydone. completion. No clinical of blood biochemical and were hematological parameters andrats organ Also, necropsy andsigns of toxicity were studies observed throughout study. and before water study consumption, bodyweight, blood histopathological were performed.the Four of 160Food rats died completion. No clinical signs biochemical andobserved hematological parameters, organ weight ratios consumption, and histopathological findings were similar of toxicity were throughout the study. Food and water bodyweight, blood in control and treated groups. The histological lesions found in and treated animals are commonly present in biochemical and hematological parameters, organ weight ratios histopathological findings were this specie and strain according to literature and our historical data.inIntreated conclusion, long-term (12 months) similar in control and treated groups. The histological lesions found animals are commonly oral treatment of rats and with strain D-004according (800-2000tomg/kg/day) didour nothistorical show evidences of D-004- related toxicity present in this specie literature and data. In conclusion, long-term under our conditions. The highest doseD-004 tested(800-2000 (2000 mg/kg) was a no-observed effect level in this (12 months) oral treatment of rats with mg/kg/day) did not showadverse evidences of D-004related toxicity under our conditions. The highest dose tested (2000 mg/kg) was a no-observed adverse study. Key words: toxicity,D-004, lipid extract, Roystonea regia fruit, Sprague Dawley rat 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-023 Reproductive Toxicity of D-004, a lipid extract from Roystonea regia fruits, in Sprague Dawley rats Gutiérrez Martínez, Ariadne; Pardo, B.; Gámez Menéndez, Rafael; Mas Ferreiro, Rosa María; Noa Puig, Miriam; Marrero, G.; Arencibia Arrebola, Daniel Francisco; García, H.; Curveco, Dayisell Lázara Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] mixture of of free fatty acids, D-004 is aa lipid lipid extract extractof ofroyal royalpalm palm(Roystonea (Roystonearegia) regia)fruits fruitsthat thatconsists consistsinina a mixture free fatty acids, areare thethe most abundant; that prevents prostate hyperplasia wherein oleic, oleic, lauric, lauric,palmitic, palmitic,and andmyristic myristic most abundant; that prevents prostate hyperplasia hashas shown to inhibit the enzyme activity of prostate 5- - 5- induced with with testosterone testosteroneininrodents. rodents.D-004 D-004 shown to inhibit the enzyme activity of prostate reductase in in vitro.vitro. Then, Then, D-004-impairment D-004-impairment of reproductive of reproductive toxicity could be toxicity expected. could be expected. Thisstudyinvestigatedon the possible reproductive toxicity of D-004 administered to fertile Sprague Dawley Dawley rats of both rats sexes. of both Rats sexes. were Ratsrandomised were randomised into four into groups: four groups: a control a control group,group, treatedtreated with the withvehicle the and vehiclewith three andD-004 three at with 500, D-004 750 and at 500, 1 000 750mg/kg/day, and 1 000respectively. mg/kg/day, respectively. Female rats were Female treated rats were for 15treated days prior for 15 days prior to mating,mating throughout mating and fromtopregnancy day 21 of lactation. Maleadministered rats were to mating, throughout and from pregnancy day 21 of to lactation. Male rats were 10 administered weeks before, and during mating. effects on growth, development, weeks before,10and during mating. The effects onThe growth, development, reproductive reproductive performance, and performance, and fertility of were the F1 generation were assessed. No evidence of D-004-related toxicity fertility of the F1 generation also assessed. No also evidence of D-004-related toxicity was observed. Thus, was observed. Thus, maternal weight and food consumption, litter the size, survival period through theweights of maternal body weight and food body consumption, litter size, survival through weaning and weaning weights of male female pups did not show differences and and male andperiod femaleand pups did not showand differences between control and treated between groups. control Drug-treated treated groups' groups. offspring Drug-treated control groups' comparable in both physical control wereand comparable in bothoffspring physical were and sensorial development and inand reproduct ive sensorial development andno in reproductive performance. There no significant among F2 performance. There were significant differences among F2 were generation groups differences in the number of born generation the number of born pups pups,survival. mean pup's weight andD-004 postnatal pups survival. In to rats of pups, meangroups pup's in weight and postnatal In conclusion, orally administered conclusion, D-004 rats of both sexes didfunction, not affect the fertility andthat general both sexes did not orally affect administered the fertility andtogeneral reproductive which indicates it does not alter reproductive which indicates it does not alterofthe the lactation, function, growth, development andthat sexual maturation thelactation, progeny.growth, development and sexual Key words: toxicity, D-004, Roystonea regia, Sprague Dawley rat 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-024 Teratogenesis of D-004 in rabbits Gutiérrez Martínez, Ariadne; Gámez Menéndez, Rafael; Mas Ferreiro, Rosa María; García, H.; Goicochea, E.; Bucarano, I. Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] El D-004 es un extracto lipídico de los los frutos frutos de de la la palma palma real real (Roystonea (Roystonea regia) regia) que que contiene contiene una una mezcla mezcla reproducible de ácidos grasos, fundamentalmente fundamentalmente oleico, oleico, palmítico, palmítico, láurico láurico yymirístico. mirístico.El Eltratamiento tratamientooral oral significativamente la la hiperplasia hiperplasia prostática prostática inducida inducidaen enroedores.El roedores.Elobjetivo objetivodel delestudio con D-004 previene significativamente estudio en evaluarel teratogénico en conejos. Las hembras preñadasse consistióconsistió en evaluarel potencialpotencial teratogénico del D-004del enD-004 conejos. Las hembras preñadasse distribuyeron distribuyeron aleatoriamente enun tres grupos: un control, tratado (tween con vehículo (tween dos D-004 aleatoriamente en tres grupos: control, tratado con vehículo 65/agua) y dos65/agua) tratadosy con tratados con mg/kg). D-004 (500 y 1000 mg/kg). Se administró producto el día por 18 de (500 y 1000 Se administró el producto desde eleldía 6 hastadesde el díael18díade6 lahasta gestación víalaoral. gestación por vía Se sacrificaron de se la examinaron gestación y sus fetos de se anormalidades examinaron Se sacrificaron lasoral. hembras el día 29 delas la hembras gestaciónely día sus 29 fetos en busca en busca de anormalidades externas, viscerales y esqueléticas.No huboentre diferencias significativas entre externas, viscerales y esqueléticas.No hubo diferencias significativas los animales tratados y los los animales tratadosal yincremento los controles cuanto al madres incremento del peso de madresdurante y el consumo de controles en cuanto delen peso de las y el consumo de las alimentos la gestación. alimentos Los grupos tratadosen y el comparables en elde número de Los gruposdurante tratadoslaygestación. el control fueron comparables el control númerofueron de cuerpos lúteos, sitios implantación, cuerpos lúteos, fetos sitios vivos, de implantación, reabsorciones, fetos vivos, de Se los fetos y proporción de loscon reabsorciones, peso de los fetos y proporción de lospeso sexos. encontraron tres fetos sexos. Se encontraron malformaciones viscerales: tres fetos uno con del malformaciones grupo control y viscerales: dos tratados unocon del 1000 grupo mg/kg. control No y dos setratados observaron con 1000 mg/kg. No se malformaciones externas o esqueléticas en externas ninguno odeesqueléticas los grupos.en Las ninguno variaciones de los grupos. esqueléticas Las observaron malformaciones variaciones esqueléticas observadasno tuvieron una observadasno incidencia estadísticamentesignificativa.Los tuvieron una incidencia estadísticamentesignificativa.Los resultados del estudio permiten concluir quedella estudio administración vía oral delaD-004 en dosis de 1000 resultados permitenpor concluir que administración por500 vía yoral de mg/kg D-004 durante en dosiseldeperíodo 500 y de organogénesis no es teratogénica o embriotóxica en conejos. Key words: teratogenesis, D-004, rabbit 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-025 Effects of D-004 and anti-oxidant and/or anti-inflammatory substances on prostatic hyperplasia induced by testosterone and phenylephrine in rats Oyarzábal Year, Ambar; Pérez Guerra, Yohani; Molina Cuevas, Vivian; Jiménez Despaigne, Sonia; Ravelo Calzado, Yazmin; Mas Ferreiro, Rosa María Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] study waswas to investigate the effects of D-004, and/or anti-inflammatory The aim aimofofthis this study to investigate the effects of antioxidant D-004, antioxidant and/or anti-inflammatory prostate hyperplasia hyperplasia (PH) (PH) induced induced by bytestosterone testosterone(T) (T)and andphenylephrine phenylephrine(PHE) (PHE)ininrats. rats.Two substances on prostate Two experiments conducted. First experiment: Rats randomized were randomized into seven a negative experiments were were conducted. First experiment: Rats were into seven groups:groups: a negative control control and six and groups six injected groups injected with T; awith positive T; a positive control and control five and were fivetreated were treated with D-004 with(400 D-004 mg/kg), (400 mg/kg), grape seed grape seed extract (GSE) extract (250 (GSE) mg/kg), (250 vitamin mg/kg), E (VE) vitamin (250 E (VE) mg/kg), (250ibuprofen mg/kg), ibuprofen (200 mg/kg) (200ormg/kg) celecoxib or celecoxib (50 mg/kg). (50 mg/kg). Second experiment: Second experiment: Rats were randomized Rats were randomized into eight groups: into eight a negative groups:vehicle a negative control vehicle and seven controlgroups and seven groups injected with PHE: injected a positive with PHE: control, a positive three treated control, with threeD-004 treated (200, with400 D-004 and(200, 800 mg/kg) 400 andand 800three mg/kg) others and others (0.4 with mg/kg), tamsulosin mg/kg), GSE mg/kg) and VE (250 mg/kg), In the with three tamsulosin GSE(0.4 (250 mg/kg) and(250 VE (250 mg/kg), respectively. In respectively. the first experiment: first experiment: Oral of D-004, of any other treatment, significantly prostate Oral administration ofadministration D-004, not of any other not treatment, significantly reduced prostatereduced weigh and prostate weigh and prostate weigh/body weighto ratio as compared the positive control. D-004, GSE and VE, not weigh/body weigh ratio as compared the positive control.toD-004, GSE and VE, not ibuprofen or celecoxib, ibuprofensignificantly or celecoxib, lowered significantly MDA values. inhibitions In contrast, inhibitions lowered prostate MDA values. Inprostate contrast, significant ofsignificant prostate MPO activityof were prostate MPO GSE, not ibuprofen andSecond celecoxib, not with VE. Second achieved with activity D-004, were GSE,achieved ibuprofenwith andD-004, celecoxib, with VE. experiment: D-004 (200-800 experiment: D-004significantly (200-800 mg/kg) significantly voided per total micturition andlowered mg/kg) increased volumeincreased voided per micturitionvolume (VM) and urinary volume(VM) (UTV), urinary total MDA volume significantly MDA in prostate and bladder significantly in (UTV), prostatelowered and bladder homogenates, and reduced carbonylhomogenates, groups (GC) and levelsreduced only in carbonyl groups (GC) levels only in significantly the prostate.VM Tamsulosin significantly VMvariables. and UTV,GSE but and the prostate. Tamsulosin increased and UTV, increased but unchanged oxidative unchanged oxidative variables. GSEVE, andnot VEGSE, unchanged the UTV, whereas VE, not GSE,the modestly but VE unchanged the UTV, whereas modestly but significantly attenuated PHE-induced significantly the PHE-induced decrease of D-004 VM. In (400 conclusions, treatment with D-004 (400 decrease of attenuated VM. In conclusions, oral treatment with mg/kg), oral but not with the antioxidant and mg/kg), but not with the antioxidant anti-inflammatory tested, prevented T-induced in anti-inflammatory substances tested,and prevented T-induced substances PH in rats, so that the preventive effect ofPH D-004 rats, on this somodel that the does preventive not seem effect to beofassociated D-004 on this to its model antioxidant does not or anti-inflammatory seem to be associated effects. to The its antioxidant single oral or anti-inflammatory administration of D-004 effects. (200-800 The single mg/kg)oral was administration the only treatment of D-004 that(200-800 ameliorated the urodynamic mg/kg) was the onlychanges and reduced oxidative variables in the prostate of rats with PHE-induced treatment thatincreased ameliorated the urodynamic changes and reduced increased oxidativePH. variables in the Key words: anti-inflammatory; antioxidant; d-004; prostate hyperplasia; urodynamic changes 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-026 Effects of oral treatment (60 days) with D-004, a lipidic extract from Roystonea regia fruits, on the rat prostate hyperplasia and oxidative markers Oyarzábal Year, Ambar; Jiménez Despaigne, Sonia; Pérez Guerra, Yohani; Molina Cuevas, Vivian; Mas Ferreiro, Rosa María; Curveco, Dayisell Lázara Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] Short-term (14 days) treatment with with D-004, D-004, aa lipid lipid extract extractfrom from Roystonea Roystonea regia regiafruits, fruits,has hasbeen beenshown showntoto prevent testosterone (T)-induced prostate hyperplasia (PH) and increased increased oxidative stress stress in in rat rat prostate, prostate, but no no study study has hasdocumented documentedif ifsuch sucheffects effectspersist persist after after longer longer treatment. treatment. ThisThis study study investigated investigated the the persistence of the the effects effects of ofD-004 D-004orally orallygiven givenfor for6060days daysforforpreventing preventingprostate prostate enlargement enlargementand and increase increase of oxidative of oxidative markers in markers rats with in T-induced rats with T-induced PH. Rats were PH. Rats randomized were randomized into three groups: into three a negative groups: acontrol negative and two T-injected control andthat tworeceived T-injectedorally that vehicle received(positive orally vehicle control) (positive or D-004 control) (400 or mg/kg/day), D-004 (400respectively. mg/kg/day), Prostate weights respectively. of positive Prostate controls weights significantly of positive andcontrols markedly significantly increasedand overmarkedly the time,increased while persistent over the while persistent reductions suchinincreases were seen D-004-treated rats. and time, significant reductionsand of significant such increases were of seen D-004-treated rats. in Also, D-004 significantly Also, D-004 of prostate reduced thesignificantly T-induced reduced increasetheofT-induced prostateincrease conjugated diene conjugated generation diene and generation sulphydril and groups sulphydril groupsachieving concentrations, achieving a complete reduction from the day 30 startingConcluding, the concentrations, a complete reduction from the day 30 after starting theafter treatment. treatment. the effects Concluding, of oral treatment the effects with D-004 of oral (400 treatment mg/kg/day) with on D-004 T-induced (400 mg/kg/day) prostate enlargement on T-inducedand prostate increased prostate oxidative markers persisted over 60 days of treatment. Key words: d-004, prostatic hyperplasia, oxidative markers, roystonea regia, testosterone 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-027 Characterization of vegetable material and oil obtained from Acrocomia crispa fruits Sierra Pérez, Roxana de la Caridad; Vicente Murillo, Roxana; González Canavacciolo, Víctor L.; Morales Rico, Carmen Luisa; Rodríguez Leyes, Eduardo Antonio; Marrero Delange, David; Tamame Tirado, Dayana Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] El D005, nuevo nuevo ingrediente ingrediente activo activo antiinflamatorio, antiinflamatorio, se seobtiene obtiene aapartir partirdel delaceite aceitede delos losfrutos frutosmaduros madurosdede Acrocomia crispa. Al Al no no existir existiruna unametodología metodología para paraelelprocesamiento procesamientodedeestos estos frutos frutos y no y no encontrarse encontrars e vegetal o del aceite nosnos reportes sobre las las características características físico-químicas físico-químicas oolalacomposición composicióndel delmaterial material vegetal o del aceite propusimos como tratamiento de de los los frutos y caracterizar el material como objetivo objetivo establecer establecerelelmétodo métododede tratamiento frutos y caracterizar el material eraera a temperatura ambiente a laa sombra vegetal y el el aceite. aceite. Se Seencontró encontróque quelalamejor mejorforma formadedesecado secado a temperatura ambiente la sombra molino de de martillos. martillos. Ocho Ocho lotes lotes de de material material veget al durante 25 días, y que la molienda debía realizarse con molino vegetal se caracterizaron se caracterizaron en cuanto en cuanto a humedad a humedad residual residual (< 8 %), (< 8 contenido %), contenido de cenizas de cenizas (totales: (totales: 2,302,303,87%; 3,87%; insolubles insolubles en HCl: en 0,70-0,97%; HCl: 0,70-0,97%; solublessolubles en agua: en0,44-1,59%), agua: 0,44-1,59%), contenido contenido de aceite de (13-18 aceite (13-18 %) y contenido %) y contenido de ácidos grasos de ácidos (totales: grasos92-96 (totales: % y libres: 92-96 % 1-3y %) libres: en el1-3 aceite. %) enAdemás, el aceite.seAdemás, obtuvieron se ocho obtuvieron lotes de ocho aceite lotes a partir de aceite de cada a partir lote de decada material lote de vegetal material con vegetal un rendimiento con un rendimiento superiorsuperior al 11 %, al 11los%,cuales los cuales fueron fueron caracterizados caracterizados encontrándose encontrándose que todos que fueron todos fueron líquidoslíquidos oleosos oleosos traslúcidos traslúcidos color amarillo-naranja color amarillo-naranja y olor y olor dulce, dulce, con pérdidas con pérdidas por secado por secado < 5 %, < 5densidad %, densidad relativa relativa (0,88-0,91 (0,88-0,91 g/ml)g/ml) e índice e índice de de refracción refracción (1,449(1,449-1,457). 1,457). El índice El de índice saponificación de saponificación estuvo estuvo entre 211-239, entre 211-239, el contenido el contenido de material de material no saponificable no < 1 %, saponificable el contenido de < 1ácidos %, el grasos contenido totales de ácidos 90 %grasos y elevados totalesíndices 90 %de y elevados acidez (48-345) índices lo deque acidez concuerda (48-345)con lo que un altoconcuerda contenido con de ácidos un altograsos contenido libresde(9-60 ácidos %).grasos Se realizó libresun(9-60 extracto %). Se metanólico realizó un a extracto un lote de material metanólico vegetal encontrándose a un lote de un material 0,007 vegetal % de polifenoles encontrándose expresado un 0,007 como %pirogalol. de polifenoles expresado como Key words: acrocomia crispa, caracterización de aceite, material vegetal 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-028 Characterization of D005 a lipidic extract from Acrocomia crispa fruits Sierra Pérez, Roxana de la Caridad; Vicente Murillo, Roxana; González Canavacciolo, Víctor L.; Morales Rico, Carmen Luisa; Rodríguez Leyes, Eduardo Antonio; Marrero Delange, David; Tamame Tirado, Dayana Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] Los fitofármacos, representan representan una una parte parte importante importante del delcomercio comerciodedemedicamentos, medicamentos,requiriendo requiriendononosolo solo de procesos de de obtención obtención reproducibles reproducibles sino sinotambién tambiéndedecontroles controlesdede calidad calidad queque permitan permitan verificar verificar el el D005, es es un un nuevo ingrediente activo compuesto por una cumplimiento de de los los atributos atributosdeseados. deseados.ElEl D005, nuevo ingrediente activo compuesto por una mezcla de ácidos grasos libres extraídos yy purificados a partir partir de de los los frutos frutos de de A. A.crispa crispa con con potente potente efecto efecto antiinflamatorio. Tomando en consideración lo el dicho, el trabajo tuvoobjetivo como objetivo caracterizar antiinflamatorio. Tomando en consideración lo dicho, trabajo tuvo como caracterizar los loteslos de lotes de D005, para establecer los intervalos ense losencuentran que se encuentran sus principales características. Se D005, para establecer los intervalos en los que sus principales características. Se encontró encontró eran oleosos líquidos oleosos traslúcidos color naranja-rojizo y oloragradable. dulce que todosque los todos lotes los eranlotes líquidos traslúcidos de colorde naranja-rojizo y olor dulce Los agradable. Los de obtención partir del aceite estuvieron del 70por %,secado las rendimientos derendimientos obtención a partir del aceitea estuvieron por encima del 70por %,encima las pérdidas <1%, pérdidas porrelativa secadoentre <1%,0,88-0,91 la densidad relativa entre de 0,88-0,91 g/mL, el índice refracción entre la densidad g/mL, el índice refracción osciló entre de 1,446 y 1,453,osciló el contenido 1,446 y 1,453, contenido de< material no saponificable < 2 encima %, el índice de acidez por encima de 350, el de material no el saponificable 2 %, el índice de acidez por de 350, el contenido de ácidos grasos contenido de ácidos libres superior al 90 %.de La ácidos composición de ácidos grasos, mostró libres superior al 90 grasos %. La composición individual grasos,individual mostró que el D005 presenta como que el D005 presenta como los componentes mayoritarios ácidos (C12:0= 34,75 %) y %) mirístico componentes mayoritarios ácidos láurico (C12:0= los 34,75 %) láurico y mirístico (C14:0=13,22 y oleico (C14:0=13,22 (C18:1= 30,41 %) %)yseguido oleico (C18:1= de los ácidos 30,41 %) palmítico seguido(C16:0= de los ácidos 8,94 %), palmítico esteárico (C16:0= (C18:0= 8,94 3,62 %),%), cáprílic o esteárico (C18:0= 3,62 %), (C8:0=1,04%), cáprico cáprílico (C10:0=2,29 (C8:0=1,04%), %), palmitoleico cáprico (C10:0=2,29 (C16:1= 0,07 %), palmitoleico %). La determinación (C16:1= 0,07 de los %).intervalos La en los que se encuentran estos en parámetros permitirá establecer las especificaciones de calidad para determinación de los intervalos los que se encuentran estos parámetros permitirá establecer las este ingrediente activo. Key words: acrocomia crispa, d005, extracto lipídico 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-029 Determination of the oil and fatty acid content in fruits of palms that grown in Cuba Vicente Murillo, Roxana; Rodríguez Leyes, Eduardo Antonio; González Canavacciolo, Víctor L.; Marrero Delange, David; Sierra Pérez, Roxana de la Caridad; Morales Rico, Carmen Luisa Centro Nacional de Investigaciones Científicas (CNIC), [email protected] Aunque se encuentran encuentran ampliamente Aunque se ampliamente distribuidas distribuidas en en Cuba Cuba yy hay hay varios varios reportes reportes de de sus sus usos usos etnomédicos, las palmas cubanas han cubanas sido poco sin embargo, cuenta en la gran variedad etnomédicos, las palmas hanestudiadas; sido poco estudiadas; sinteniendo embargo,enteniendo cuenta la gran de palmas existentes en Cuba y lo factible que pudiera resultar el empleo de sus frutos para la obtención variedad de palmas existentes en Cuba y lo factible que pudiera resultar el empleo de sus frutos para lade productos naturales con usos nutracéuticos y/o farmacéuticos; resulta interesante realizar realizar estudios obtención de productos naturales con usos nutracéuticos y/o farmacéuticos; resulta interesante fitoquímicos preliminares a algunasa de las especies nativas o nativas introducidas del país. del Parapaís. la realización estudios fitoquímicos preliminares algunas de las especies o introducidas Para la de este trabajode seeste recolectaron maduros frutos frescos de S. repens, regia, Sabal palmetto, Sabal realización trabajo sefrutos recolectaron maduros frescosRoystonea de S. repens, Roystonea regia, Sabal maritima, Colpothrinax Thrinax whritii, radiata, Veichia merrillii, Chrysalidocarpus lutescen, Wotyetia palmetto, Sabal maritima,whritii, Colpothrinax Thrinax radiata, Veichia merrillii, Chrysalidocarpus bifurcata, Caryota bifurcata, urens y Phytosperma Los frutos se secaron a temperatura a la lutescen, Wotyetia Caryota urenselegans. y Phytosperma elegans. Los frutos se secaron aambiente temperatura sombra 20 días, se molieron se molieron sometieron extracción con hexano en extractos ambientedurante a la sombra durante 20 días,y se y sea sometieron a extracción consöxhlet. hexanoLos en söxhlet. lipídicos se secaron y se determinaron rendimiento el derendimiento extracción yde sus contenidos de ácidos grasos Los extractos lipídicos seles secaron y se les el determinaron extracción y sus contenidos de como metílicos pormetílicos cromatografía de gases. de Estos extractos presentaron rendimientos de ácidos ésteres grasos como ésteres por cromatografía gases. Estos extractos presentaron extracción entre 0,6 y 25,5 %; contenido de %; ácidos grasosdetotales hasta 92,5 58,4 % y hasta contenidos rendimientos de extracción entre 0,6 y 25,5 contenido ácidosdesde grasos58,4 totales desde 92,5 de libres de entre 1,9 ylibres 70,2 entre %. La1,9 composición de composición ácidos grasos como ácidos mayoritarios % yácidos contenidos ácidos y 70,2 %. La depresentó ácidos grasos presentó como en las todas las especies el palmítico (9,2-52,2 el %)palmítico y el oleico(9,2-52,2 (9,6-42,7); que los ácidos ácidos mayoritarios en lasestudiadas todas las especies estudiadas %) mientras y el oleico (9,6-42,7); linoleico 3,4ácidos %) y esteárico fueron los minoritarios. Losfueron ácidos mirísticoLos y linoleico mientras (0,1 que -los linoleico(1,7 (0,1-6,1 - 3,4%)%) y esteárico (1,7 -6,1 %) losláurico, minoritarios. ácidos fueron en unasfueron especies y minoritarios paraespecies otras. El presente trabajo láurico,mayoritarios mirístico y linoleico mayoritarios en unas y minoritarios para permitió otras. Elcaracterizar presente las fracciones de las once especieslipídicas de arecaceas la isla, lopresentes cual contribuyó trabajo permitió lipídicas caracterizar fracciones de once presentes especies deenarecaceas en la isla,al conocimiento de algunas de las principales características físicas y químicas, con vistas a su lo cual contribuyó al conocimiento de algunas de las principales características físicas y químicas, posible con empleo en la industria farmacéutica. Key words: palmas cubanas, aceite, ácidos grasos, frutos de arecaceae 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-030 Fatty acids profile of two species of Lamiaceae that grown in Cuba Marrero Delange, David; Morales Rico, Carmen Luisa; González Canavacciolo, Víctor L.; Sierra Pérez, Roxana de la Caridad; Rodríguez Leyes, Eduardo Antonio; Vicente Murillo, Roxana Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] The Lamiaceae family has been characterized by the occurrence of unsaturated acids acids in their their seed oils. The importance of of such such acids acids as as chemotaxonomic chemotaxonomic markers, markers,for forthe thecosmetic, cosmetic,nutritional nutritionaland andmedicinal medicinal also been been demonstrated. demonstrated. Salvia Salvia coccinea coccineaL., L.,known knownasasbanderilla banderillaand andLeonotis Leonotisnepetaefolia industries has also nepetaefolia (L.) R.Br., knowndeasSan Bastón de Sanare Francisco are two thisare family and cultivated are mainly (L.) R.Br., known as Bastón Francisco two members of members this familyofand mainly cultivated Cuba as ornamental plants. The of purpose of this was to determine acid in Cuba asinornamental plants. The purpose this work waswork to determine the fatty the acidfatty composition of composition of seed oil of these Seeds were collected, milled and seed oil of these species. Seedsspecies. were collected, milled and extracted withextracted hexane. with Thehexane. extractsThe were extractsand werethe filtered andwas the evaporated solvent was under evaporated under reduced in a rotary evaporator. filtered solvent reduced pressure in pressure a rotary evaporator. Organolept ic Organoleptic characteristics characteristics and extractionand yields extraction of the oils yields were of the determined. oils were The determined. content of The fatty content acids,ofanalysed fatty acids, as analysed methyl esters, as methyl were determined esters, werebydetermined GC-FID and byGC/MS. GC-FID The andmain GC/MS. fatty The acidsmain found fatty in the acids oil of found S. coccinea in the oil of S. coccinea were linolenic (33.1%), were linolenic linoleic(33.1%), (25.2%)linoleic and oleic (25.2%) (13.3%) andacids; oleic whereas (13.3%) acids; in L. nepetaefolia whereas inoil L. were nepetaefolia found oil were oleic (43.2%), found laballenic oleic (43.2%), (18.4%), laballenic palmitic (18.4%), (15.0%), palmitic and linolenic (15.0%), (13.4%) and linolenic acids. Other (13.4%) fatty acids. acidsOther were also fatty found but inalso minor proportions. Theproportions. results of this areofathis contribution the chemicaltocomposition acids were found but in minor Thestudy results study are to a contribution the chemical knowledge of both species and suggest that such oils could be used in several industries. Key words: salvia coccinea, leonotis nepetaefolia, lamiaceae, fatty acid, gas chromatography 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-031 Study of the methanolic extract from Terminalia catappa leaves Marrero Delange, David; Morales Rico, Carmen Luisa; González Canavacciolo, Víctor L.; Vicente Murillo, Roxana Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] Varias de delas lasespecies especies deldel género género Terminalia Terminalia (Combretaceae) (Combretaceae) han demostrado han demostrado internacionalmente internacionalment e cardioprotectoras, hipolipemiantes, vasodilatadoras, diuréticas, propiedades farmacológicas farmacológicascomo como cardioprotectoras, hipolipemiantes, vasodilatadoras, diuréticas, propiedades antioxidantes, gastroprotectoras, gastroprotectoras, hepatoprotectoras, hepatoprotectoras, antiinflamatorias antiinflamatorias y yanalgésicas. analgésicas.Estas Estas propiedades han sido atribuidas glicósidos, encontrados en en las las hojas y la y la atribuidas fundamentalmente fundamentalmente aalos lospolifenoles polifenolesy sus y sus glicósidos, encontrados hojas catalogada corteza. Teniendo en en cuenta cuenta además, además, que que en enCuba Cubalalaespecie especieTerminalia TerminaliaCatappa Catappaeses catalogadacomo como su su composición química y estudios una planta invasora invasora yyque queexisten existenpocos pocostrabajos trabajosrelacionados relacionadoscon con composición química y estudios farmacológicos, se llevó aa cabo cabo un un estudio estudio preliminar preliminar para paradeterminar determinarácidos ácidospolifenólicos polifenólicosenenelelextracto extracto molidas, desgrasadas concon hexano y y metanólico de de sus sus hojas. hojas. Las Lashojas hojasfueron fueronrecolectadas, recolectadas,secadas, secadas, molidas, desgrasadas hexano posteriormente extraídas con metanol en un baño ultrasónico. ultrasónico. El El extracto extracto se se filtró filtró yy el el disolvente disolvente se seeliminó eliminó vacío. El extracto seco se hidrolizó y se extrajo con acetato de etilo. Se determinó el al vacío.al El extracto seco se hidrolizó y se extrajo con acetato de etilo. Se determinó el rendimiento de rendimiento las de extracción, las características y los totales polifenoles totales el mediante método extracción, características organolépticasorganolépticas y los polifenoles mediante métodoelde Follinde Follin-Ciocalteu, como la composición química porprevia CG/EM previa formación de derivados TMS. Se Ciocalteu, así como así la composición química por CG/EM formación de derivados TMS. Se obtuvo un obtuvo de líquido un color líquidopardo de color rojizo pardo oscuro rojizo de olor oscuro característico de olor característico y con un rendimiento y con un rendimiento de extracción de extracción de 10,8%. El de 10,8%. El contenido total contenido de polifenoles total de polifenoles fue 184,6fue (mg 184,6 Pirogalol/100g (mg Pirogalol/100g Extracto) Extracto) y como y como ácidos ácidos polifenólicos polifenólicos mayoritarios mayoritarios se encontraron se encontraron el ácido gálico el ácido(3,4,5-trihidroxibenzoico), gálico (3,4,5-trihidroxibenzoico), ácido vanílico ácido vanílico (3- metoxi-4(3metoxi-4-hidroxibenzoico), hidroxibenzoico), ácido 3,4-dihidroxibenzoico, ácido 3,4-dihidroxibenzoico, ácido 2,5-dihidroxi-benzoico, ácido 2,5-dihidroxi-benzoico, ácido 4ácido hidroxibenzoico 4y hidroxibenzoico ácido benzoico. También se estructuras detectaron otras estructuras polifenoles. ácido benzoico. yTambién se detectaron otras relacionadas conrelacionadas polifenoles. con La presencia de La presencia de estos compuestos justificar las propiedades medicinales atribuidas estos compuestos pudiera justificarpudiera las propiedades medicinales atribuidas a esta especie, aaesta la vez que servirían de base para continuar con futuras pruebas farmacológicas que avalen sus usos etnomédicos. Key words: terminalia catappa, combretaceae, ácido gálico, polifenoles, cg/em, tms 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-032 Determination of arsenic in natural products by hydride generation - atomic absorption spectrometry Luna Saucedo, Bárbara E.; Quevedo Álvarez, Odalys; Fernández Ruíz, Rosabel; Gómez Pascual, Jorge; Estrada la Rosa, Tamara; Sánchez Sotolongo, Elsa Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] The national and international international regulator regulator organisms organisms demand, demand, for for the theuse useof ofnatural naturalproducts productslike likenutritional nutritional of of some toxic elements for men, suchsuch as the supplements or or drugs, drugs, to toknow knowwith withaccuracy accuracythe thecontent content some toxic elements for men, as the Arsenic (As). The Policosanol, Prevenox Prevenox and and Abexol Abexol are are actives actives pharmaceuticals pharmaceuticals ingredients ingredients extracted extractedofof the wax of the sugar cane cane and and bees bees and and they they are are constituted constitutedby bymixtures mixturesofofalcohols alcoholsororaliphatic aliphaticacids, acids,both both high with with high molecular molecular weight, weight, showing showing cholesterol cholesterol lowering, lowering, antioxidant antioxidant andand gastroprotective gastroprotective effects. effects. In the In the present present study, study, a microwave-assisted a microwave-assisted digestion digestion technique technique (HNO3(HNO3H2O2, H2O2, EPA EPA 3052) 3052)was wasemploy employfor forthe the determination determination of total of total arsenic arsenic in in thethe three threematrices matricesofofnatural natural products products by by Hydride Generation Generation --Atomic Atomic Absorption Spectrometry. determination waswas evaluated by the additions Spectrometry. The Theaccuracy accuracyofofthethe determination evaluated by standard the standard additions by three three samples samples between between 95% 95% and and105 105. .The Thedetection detectionlimit limitwas method. The recoveries were satisfactory by was 0.002 µg.g-1. content of in Asthe in the three analysed natural products were under method 0.002 µg.g-1. The The content of As three analysed natural products were under thethe method detection detection limit as the established of the United States Pharmacopeia Ed. for similar limit as well asas thewell established limit of thelimit United States Pharmacopeia 33 Ed. for 33 similar products. Key words: arsenic, antioxidants, hydride generation, atomic absorption spectrometry 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-033 Characterization of by-product from the obtaining process of propolis for its possible use as nutritional supplement Martínez Rojas, José Manuel; Cabrera Rabí, Leila; Fernández, Jaquelin; Urra, Caridad; Fajardo, Mario; M. Pérez, Adolfo; Falero Morejón, Alina Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] El propóleo es un producto natural que las abejas elaboran a partir de exudados de diversas plantas. Es El propóleo es un producto natural que las abejas elaboran a partir de exudados de diversas plantas. Es usado por las abejas para recubrir las paredes de la colmena y mantenerla libre de bacterias. Está usado por las abejas para recubrir las paredes de la colmena y mantenerla libre de bacterias. Está compuesto básicamente por sustancias resinosas procedentes de distintas especies vegetales con un compuesto básicamente por sustancias resinosas procedentes de distintas especies vegetales con un 50% de bálsamos y resinas, 35% de cera, 10% de aceites esenciales y un 5 % de polen. Este material 50% de bálsamos y resinas, 35% de cera, 10% de aceites esenciales y un 5 % de polen. Este material resinoso es sometido a una extracción hidroalcohólica para la obtención de tintura de propóleos y extracto resinoso es sometido a una extracción hidroalcohólica para la obtención de tintura de propóleos y blando. Como subproducto de este proceso se obtiene un residuo ceroso que se saponificó, se extrajo extracto blando. Como subproducto de este proceso se obtiene un residuo ceroso que se saponificó, se con acetona y se purificó empleando seis combinaciones de hexano-acetona. Los mejores resultados se extrajo con acetona y se purificó empleando seis combinaciones de hexano-acetona. Los mejores encontraron después de dos purificaciones con hexano y tres con acetona, obteniéndose una resultados se encontraron después de dos purificaciones con hexano y tres con acetona, obteniéndose concentración de 94 % de una mezcla de alcoholes de alto peso molecular, donde predominan los una concentración de 94 % de una mezcla de alcoholes de alto peso molecular, donde predominan los alcoholes C30 y C32. El objetivo de este trabajo fue caracterizar este residuo ceroso y evaluar su posible alcoholes C30 y C32. uso como suplemento alimentario, teniendo en cuenta las propiedades conocidas de los alcoholes El objetivo de este trabajo fue caracterizar este residuo ceroso y evaluar su posible uso como presentes en la cera de abejas y la cantidad de subproducto generado en la obtención de tintura de suplemento alimentario, teniendo en cuenta las propiedades conocidas de los alcoholes presentes en la propóleos y extracto blando. cera de abejas y la cantidad de subproducto generado en la obtención de tintura de propóleos y extracto Key words: propolis, nutritional supplement, wax 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-034 Study of the behavior of oral Oleozón manufactured in a new production place under conditions of accelerated stability Bécquer Romagoza, Clara; Mosquera Sierra, Javier; Rodríguez Lavañino, Jean M.; Sánchez Sánchez, Dennis; Mediacejas Maletá, Yuleikis; Kindelán Mallet, Mylene Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] Introducción: El Elaceite aceitedede girasol girasol ozonizado ozonizado OLEOZON® OLEOZON® Oral Oral es un es medicamento un medicamento que presenta que presenta excelentes propiedades en en la la parasitosis parasitosis por por Giardia Giardia Lamblia. Lamblia.En Eneste estetrabajo trabajose sepresenta presentaelelestudio estudiodede estabilidad acelerada de tres lotes de producción de OLEOZON® Oral a partir de la la puesta puesta en en marcha marcha de de la planta de de producción producción de deaceite aceiteozonizado ozonizadoCNIC CNICy yteniendo teniendoenencuenta cuenta laslas indicaciones indicaciones deldel CECMED. CECMED. Materiales y Métodos: Se Se utilizaron utilizaron frascos frascos plástico plásticode debaja bajadensidad densidady yde devidrio. vidrio.Los Loslotes lotesenenestudio estudiosese mantuvieron con las las condiciones condiciones requeridas requeridas aauna una Temperatura Temperatura de de(25 (25±2)0C ±2)0C YY60% 60%dede Humedad. Humedad.SeSe analizaron durante tiempo 0, 1, 2, 3 y 66 meses meses las las características características de de calidad: calidad: índice índice de de peróxido, peróxido, índice índice de de acidez, concentración aldehídos,viscosidad, viscosidad,características características organolépticas organolépticas yy límite microbiano. acidez, concentración dede aldehídos, microbiano. Se Se Origin para el análisis de los Resultados y utilizó computadora computadora yyelelprograma programaestadístico estadístico Origin para el análisis deresultados. los resultados. Resultados y Discusión: Durante los seis meses de mantener la muestra en estudio estudio acelerada en frascos frascos de plástico plástico y medida que transcurre el tiempo el valor de de de vidrio se se ha ha podido podido apreciar apreciar que queelelíndice índicededeperóxido peróxidoa a medida que transcurre el tiempo el valor para cada unouno de de los los lotes, la la concentración en el el tiempo tiempo cero cerodisminuyó disminuyó, ,elelíndice índicededeacidez acidezaumentó aumentó para cada lotes, viscosidad y la concentración en aldehídos aldehídos volátiles volátiles se se mantuvo mantuvo cercana cercana alallímite límiteinferior inferior de deespecificación, especificación, no hubo crecimiento no hubo microbiano crecimiento paramicrobiano ninguno depara los lotes ninguno estudiados de los lotes y el comportamiento estudiados y el en los diferentes comportamiento envases es similar. Conclusiones: El producto es similar. en condiciones Conclusiones: de Temperatura El producto en decondiciones (25 ±2)0C yde60% de en los diferentes envases Humedad mantiene sus especificaciones de calidad hasta tres meses dede elaborado en las Temperatura de (25 ±2)0C y 60% de Humedad mantiene suslos especificaciones calidad hasta losnuevas tres condiciones de producción. Key words: oleozon, estabilidad acelerada 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-035 ”Medicinal Plants” Interactive Guide. Guía interactiva "Plantas Medicinales" Melgarejo López, Nelson; Álvarez Bustamante, Guadalupe; Alonso Abad, Antonio Facultad de Ciencias Médicas "Dr. Salvador Allende", Cuba [email protected] edición digital GuíaInteractiva Interactiva“Plantas “PlantasMedicinales” Medicinales”representa representalalaobra obrade dedos dosmédicos médicos yyun un ingeniero La La edición digital Guía ingenierocubanos, agrónomo, cubanos, en interesados en ponerdeal los servicio de los profesionales la salud, y de los agrónomo, interesados poner al servicio profesionales de la salud, de y de los lectores en lectores general, unapráctica herramienta el empleo racional medicinales. de las plantas general, unaenherramienta para elpráctica empleo para racional de las plantas La medicinales. temática abordada se presenta con un lenguaje claro y sencillo, al alcance de todas aquellas personas interesadas en los La temática se presenta consalud un lenguaje y sencillo, al alcance aquellas beneficios que abordada su uso reporta sobre la humana.claro De una manera resumidadey todas didáctica, se refleja en personas interesadas en los beneficiossobre que su uso reporta sobre la salud humana. esta multimedia información actualizada cuarenta y seis de las plantas medicinales reconocidas por las autoridades reguladoras de productos naturales y aprobadas por el Programa Nacional para el Desarrollo una manera resumida y didáctica, seyrefleja en estaGran multimedia sobre y laDe Generalización de la Medicina Natural Tradicional. parte deinformación las plantasactualizada incluidas, son conocidas cuarenta yen seis de las plantas reconocidas lasdeautoridades reguladoras de productos y empleadas el mundo con muymedicinales buenos resultados en el por alivio ciertas dolencias y malestares comunes; naturales y aprobadas en porcualquier el Programa parasolo el Desarrollo y la Generalización de la Medicina pueden ser encontradas latitudNacional del planeta, con la distinción de que en muchas localidades Natural el y Tradicional. y países, nombre común cambia por la influencia de culturas y costumbres, no obstante mantienen sin variación su nombre científico; tampoco sufren modificación significativa los principios activos y componentes parte de por las plantas sonsuconocidas y empleadas en en este el mundo con se muy buenosademás resultados de Gran las mismas, lo cual incluidas, se conserva carácter terapéutico; material reflejan los en el alivio de por ciertas dolencias y malestares comunes; pueden ser encontradas en cualquier su latitud delde principales usos aparatos y sistemas, las formas naturales o farmacéuticas de presentación, modo planeta, solo con la distinción de que en y países, el su nombre comúnycambia por la empleo, la dosificación, las precauciones quemuchas deben localidades tenerse en cuenta para elaboración administración; influencia de culturas y costumbres, no además, obstante de mantienen sin variación su nombre científico; tampocode entre otros aspectos de interés. Dispone una galería de imágenes que refleja las plantas sufrenunmodificación significativa los principios componentes de las mismas, lo cual se interés, índice de formulas farmacéuticas y unactivos breve yglosario para facilitarle al lector por no especializado la conserva sudecarácter este como material se reflejan los principales aparatos comprensión algunosterapéutico; términos. en Surge resultado de además la necesidad de disponer materialy usosde porun sistemas, naturales o farmacéuticas de presentación, su modo empleo, la dosificación, las integrador enlaslaformas docencia de pregrado y postgrado para la formación de de profesionales de las ciencias precauciones que deben tenerse en cuenta elaboración administración; entre otros médicas en la Atención Primaria de Salud ya quepara los su existentes hastay el momento en nuestro país,aspectos muestran interés. de una forma muy dispersa y fraccionada. Hasta la actualidad se han realizado diversos losde contenidos Dispone además,endeeluna galería demedicina imágenesnatural que refleja las plantas de interés, relacionados un índice de formulas formulas estudios científicos ámbito de la y tradicional especialmente con plantas farmacéuticas y un brevenoglosario parade facilitarle al lector lectorque no especializado especializado la comprensión comprensión de algunos algunos medicinales, sin embargo, se dispone una publicación oriente adecuadamente su manejo, objetivo para facilitarle al no la de quetérminos. nos trazamos con nuestra guía encaminada a dotar a profesionales y promotores de salud, de un instrumento práctico y eficiente en su capacitación. Surge como resultado de la necesidad de de disponer disponer de de un un material material integrador integrador en en la la docencia docencia de de pregrado pregrado y postgrado para la formación de profesionales de las las ciencias ciencias médicas médicas en en la la Atención Atención Primaria Primaria de de Salud Salud ya que los existentes hasta el momento en nuestro país, muestran los contenidos de una forma muy Key words: Medicinal Plants, Interactive Guide, Guía interactiva, Plantas Medicinales dispersa y fraccionada. Hasta la actualidad se han realizado diversos estudios científicos en el ámbito de la medicina natural y tradicional especialmente relacionados con plantas medicinales, sin embargo, no se dispone de una publicación que oriente adecuadamente su manejo, objetivo que nos trazamos con nuestra guía encaminada a dotar a profesionales y promotores de salud, de un instrumento práctico y eficiente en su 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-036 Evaluation of the anti-inflammatory activity of Sedum praealtum (always live) coming from the municipality of San Martín de las pirámides, state of Mexico Berdeja Martínez, Blanca Margarita; Vargas Díaz, M. Elena; Santacruz Ramírez, Ariadna; Rivero Ramírez, Nora Lilia; Ávila Uribe, Margarita Micaela Instituto Politécnico Nacional, México [email protected] Introduction: the the main main objective objective ofofthe theinflammatory inflammatoryreaction reactionis is delete delete or or dilute dilute thethe injurious injurious agent agent withwith a a series of humoral and cellular events that preferably preferably heal heal and and rebuild rebuild to to the the injured injured tissue tissue(Howard, (Howard, 1996). 1996). Most commonly used used nonsteroidal nonsteroidal anti-inflammatory anti-inflammatory agents agentsare arethe thesteroids steroidsand andNSAIDs, NSAIDs,both both with with very very serious adverse effects effects (Rang, (Rang, 2008) 2008)objective: objective:totoevaluate evaluatethe theanti-inflammatory anti-inflammatoryactivity activity of of thethe aqueous aqueous extract of Sedum Sedum praealtum praealtum Methodology: extract is prepared by infusion, took dry sprinkler, 6 groups of rats Methodology: each with 6extract were distributed is preparedasby(1) infusion, witnesstook without dry sprinkler, trading), (3) 6 groups treated of with rats(50(, each 200 with and 6 were 400 mg/kg of the extract),as(5)(1)with diclofenac mg/kg), (3) (6) treated positivewith control, inflammation was of induced by granuloma distributed witness without(4trading), (50(,an 200 and 400 mg/kg the extract), (5) with the and diclofenac Administration (4 mg/kg), was(6)orally positive for seven control, days, an inflammation at the end iswas dissected inducedgranulomas, by granuloma stomach and the and blood Administration samples. Results: wasphytochemical: orally for sevenflavonoids, days, at the alkaloids, end is dissected saponins granulomas, and tannins; stomach pharmacological, and bloodyou notice samples. an anti-inflammatory Results: phytochemical: process dose-dependent flavonoids, alkaloids, to exist significant saponins and differences tannins; with pharmacological, the positive control you and notice with diclofenac, an anti-inflammatory the histologically process showed dose-dependent a more severe to exist irritation significant of the mucosa differences in stomach with thewith positive diclofenac, controland Melo andcols withisolated diclofenac, four flavonoids the histologically with anti-inflammatory showed a more activity severe of irritation this plant, of the which mucosa trapsin radicals stomachand with diclofenac, antioxidants areMelo considered and colsfree isolated (Marcano four flavonoids et al., 2002). with The anti-inflammatory aqueous extract activity of Sedum of this plant, praealtum whichDC, traps radicals and antioxidants are considered free (Marcano et al., 2002). The aqueous extract of Sedum presented anti-inflammatory effect. praealtum DC, presented anti-inflammatory effect Key words: anti-inflammatory activity, Sedum praealtum, always live, San Martín de las pirámides, México 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-037 Evaluation of the antibabesic activity of three plant species in mice infected with Babesia microti Hernández de Jesús, María de Lourdes; Aguilar Figueroa, Blanca Rosa; Oble Hernández, Abraham Instituto Politécnico Nacional, México [email protected] Human babesiosis isis an an emerging emerging zoonotic zoonoticdisease diseasecaused causedbybyBabesia Babesiaspp. spp. Haveimportance importanceto to public Have public health and veterinary since it has economic impact by affecting the production of cattle. In Central America America and México, and human Mexico,cases human have cases been have confirmed been confirmed as in South as inAmerica South America and Brazil. andThe Brazil. infection The infection of humans of humans and animals and occurs animalsthrough occurs the through bite of the infected bite of ticks infected inoculated ticks inoculated sporozoites sporozoites that become that trophozoites become and trophozoites the reproduce, and erythrocyte reproduce, the rupture, erythrocyte releasing rupture, merozoites releasing merozoites that invadethat other invade erythrocytes, other erythrocytes, another another mechanism mechanism of transmission of transmission is through is through transfusion. transfusion. Treatment regimes, depending on the degree of B. microti infection is used: azithromycin with clindamycin and atovaquone with quinine. These drugs cause in Treatment patient sideregimes, effects so depending do not complete on the degree their treatment, of B. microti resistant infection strains is used: causing. azithromycin An alternative with is to search clindamycin for plants with andantiparasitic atovaquone activity. with quinine. In this These paper drugs the cause antiparasitic in patient activity side effects of the so methanol do not complete extracts of their treatment, Ibervillea sonorae, resistant Krameria strains sonorae causing. and An Phoradendrom alternative is californicum to search forinplants mice infected with antiparasitic with Babesia activity. microti In was evaluated. Parasitemiaactivity curve was determined the effect of the extracts evaluated for 33and days. this paper the antiparasitic of the methanol and extracts of Ibervillea sonorae,was Krameria sonorae The results werecalifornicum subjected in to mice statistical analysis and Zigma Zigma Plot. Observed that two extracts Phoradendrom infected with Babesia microti wasStat evaluated. showed activity in different degree. So we can conclude that plants are a potential source of chemical Parasitemiawhich structures, curvecould was determined be an alternative and the in controlling effect of theparasites. extracts was evaluated for 33 days. The results were subjected to statistical analysis and Zigma Zigma Stat Plot. Observed that two extracts showed activity in different degree. So we can conclude that plants are a potential source of chemical structures, Key words: antiparasitic activity , babesia microti , plant species 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-038 Determination of adsorption capacity of textile dyes by Peanut shell (Arachis hypogaea) and parchment coffee (Coffea arabica) Hernández de Jesús, María de Lourdes; Barragán Huerta, Blanca Estela; Flores Vargas, Eliud Fernando Instituto Politécnico Nacional, México [email protected] The textile industry is is one one of of the theactivities activities that thatgenerate generatemore morepollution pollutiondue duetotothe thelarge largevolumes volumesofofwater water used in their different processes. Among the the contaminants contaminants in in effluents effluents are are textile textiledyes, dyes,which whichdo donot notexhibit high to living organisms, however, the the strong coloring cometotoabolish abolishthethe exhibittoxicity high toxicity to living organisms, however, strong coloringthat thatthem them may come photosynthetic process. process. The The adsorption adsorptionbybyactivated activatedcarbon carbonis is one of the most used processes in the one of the most used processes in the purification of textile effluents; effluents; however however the the high high cost costofofthis thismaterial materialhas hasled ledtotoresearch researchinto intonew newadsorbent materials, including agro-industrial waste. Inwaste. this work, peanut shell and coffeecoffee were adsorbent materials, including agro-industrial In thisthe work, the peanut shellparchment and parchment were characterized characterized as adsorbents as adsorbents for Orange for Orange II (NII), II (NII), AcidAcid BlueBlue 113 113 (AA(AA 113)113) andand Basic Basic Green Green 4 (VB4). 4 (VB4). The The wastes wastes werewere ground, ground, sieved sieved and subjected and subjected to different to different pre-treatments pre-treatments (acidic, (acidic, basicbasic and and microwave), microwave), and and then then the kinetic the kinetic adsorption adsorption studies studies werewere performed. performed. Of the Of the three three dyesdyes tested, tested, onlyonly AA AA 113113 andand VB4VB4 were were adsorbed adsorbed by both by materials both materials duringduring the first the 6first hours 6 hours of treatment. of treatment. Optimization Optimization of dye of dye adsorption adsorption process process was conducted was conducted using a central using acomposite central composite design by design analyzing by analyzing the effecttheofeffect exposure of exposure time (2 and time10 (2 and min) 10 min) and power and(10 power to 50%) (10 to of 50%) the microwaves of the microwaves pretreatment pretreatment on the adsorption on the adsorption capacity.capacity. It was determined It was that the removal that of dye the residue whenincreased the powerwhen and the the power microwave exposure time had the determined the by removal of dye increased by the residue and the microwave higher value. Key words: adsorption, waste, coffee, peanuts, textile dyes, microwave 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-039 In Vitro Activity of some fractions from exctract of Ruta graveolens against Trypanosoma cruzi NINOA strain Hernández de Jesús, María de Lourdes; Ambriz Hernández, Ana Lizbeth; Aguilar Figueroa, Blanca Rosa; Barragán Huerta, Blanca Estela Instituto Politécnico Nacional, México [email protected] Trypanosoma.cruzi causes Chagas disease. It is estimated that that 11–18 11–18 million million people people in in South South America America are infected by this disease and and 100,000 people are at risk contracting it each year. year. The chemotherapy used are infected by this disease 100,000 people are at of risk of contracting it each The chemotherapy to treat has serious side effects and its efficacy on theonchronic phasephase of disease is stillisa still matter used to this treatillness this illness has serious side effects and its efficacy the chronic of disease a of debate. Previous studies have shown trypanocidal effect of Ruta graveolens. The objective of this work matter of debate. was to find the have activeshown fractions, against effect T. cruzi straingraveolens. NINOA The in vitro evaluation, after was making Previous studies trypanocidal of Ruta objective of this work to a chromatographic separation chloroform extract. Bioassay-guided phytochemical study of the find the active fractions, againstof T.the cruzi strain NINOA in vitro evaluation, after making a chromatographic chloroform extractseparation of R. graveolens of the chloroform was carriedextract. out using as toxicity bioassay Artemia salina. The in vitro Bioassay-guided trypanocidal assays phytochemical were carried study out according of the chloroform to the protocol extract reported of R. graveolens elsewhere,was withcarried slight modifications. out using as toxicity bioassay Bloodstream trypomastigotes Artemia salina. were Thequantified in vitro trypanocidal by the method assays of were Pizzi.carried The chloroform out according extract to the showed a protocol 67% mortality reported Artemia elsewhere, salina,with wasslight separated modifications. by column Bloodstream chromatography. trypomastigotes Fractionswere 232,quantified 234, 306,by311, the method 328 and 397ofwhich Pizzi.presented the lowest LC50 for biotoxicity test were selected. Fractions 306 and 328 for The a second chloroform chromatographic extract showed separation a 67% mortality were chosen, Artemiaobtaining salina, was fractions separated 292-318 by column and 340-390 which chromatography. presented an LC50 Fractions less. Of232, fractions 234, 306, tested 311, against 328 and Trypanosoma 397 which presented cruzi was the found lowest that LC50 the fraction for from biotoxicity 340 to 390 test at awere concentration selected. of Fractions 100 ug 306 / ml showed and 328 higher for a second percentchromatographic lysis against Nifurtimox separation reference were drug chosen, obtaining 292-318 and 340-390 which presented an LC50 less. Of fractions tested concentrations 50,fractions 25 and 100 ug / mL. against Trypanosoma cruzi was found that the fraction from 340 to 390 at a concentration of 100 ug / ml Key words: trypanosoma cruzi, trypanocidal activity, ruta graveolens 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-040 Evaluation of the in vitro effect of plant extracts of Ruta graveolens and Euphorbia serpyllifolia on Trypanosoma cruzi strains NINOA and INC-5 Hernández de Jesús, María de Lourdes; Montes de Oca, David de la Rosa; Aguilar Figueroa, Blanca Rosa; Barragán Huerta, Blanca Estela Instituto Politécnico Nacional, México [email protected] Chagas disease diseasecaused caused by protozoan the protozoan Trypanosoma is life-threatening, treatment with by the Trypanosoma cruzi is cruzi life-threatening, treatment with Benznidazole and Nifurtimox base ensures ensures no no cure cure for for the the disease, disease,causing causingside sideeffects effectscausing causingpatients patients to discontinue treatment. In this this work, work, in in vitro vitro trypanocidal trypanocidal activity activity of ofmethanol methanol extracts extractsofofRuta Rutagraveolens graveolens and Euphorbia serpyllifolia on Trypanosoma Trypanosoma cruzi cruzi strains strains Ninoa Ninoa and and INC INC -- 55 was was determined. determined. The The strains strains were maintained maintained bybyserial serial passage in CD1 mice, inoculated intraperitoneally with blood. infected blood. passage in CD1 mice, inoculated intraperitoneally with infected Determining the trypanocidal effect in vitro was performed by placing blood trypomastigotes with plant Determining extracts and the reference trypanocidal drugs effect to a final in vitro concentration was performed of 10, by 25, placing 50 and blood 100trypomastigotes mg / mL. Percent with lysis plant was extracts andby determined reference the method drugsoftoPizzi, a final parasite concentration numbers of 10, that 25,survived 50 and 100 the mg treatment, / mL. Percent comparing lysis with was the determined negative control. by theWith method Ninoaof strain Pizzi, showed parasite Ruta numbers graveolens that survived a high the percentage treatment,ofcomparing lysis, whilewith Euphorbia the negative control. serpyllifolia had no effect, Benznidazole obtained a percentage of 55,25,20 and 5 %, Nifurtimox 47,29,10 and 3%. The INC- 5 strain, Ruta graveolens obtained a low percentage of lysis, while Euphorbia serpyllifolia With Ninoa showed little strain activity, showed Benznidazole Ruta graveolens was 28,a 18, high13 percentage and 2 %,ofNifurtimox lysis, while was Euphorbia Euphorbia 81, 72, serpyllifolia 62 and 52%. had had The no effect, Benznidazole methanol extract Ruta graveolens percentage of ofhas 55,25,20 greaterand and activity %,than Nifurtimox Benznidazole 47,29,10 and and Nifurtimox 3%.The The on obtained a trypanocidal percentage 55,25,20 55%, Nifurtimox 47,29,10 and 3%. INC5 strain, Ruta a low percentage of lysis, while Euphorbia serpyllifolia showed Trypanosoma cruzi graveolens strain Ninoaobtained concentrations tested. little activity, Benznidazole was 28, 18, 13 and 2 %, Nifurtimox was 81, 72, 62 and 52%. The methanol extract Ruta graveolens trypanocidal has greater activity than Benznidazole and Nifurtimox Key words: ruta graveolens, euphorbia serpyllifolia, trypanosoma cruzi 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-041 Nutraceutical quality components of microencapsulated noni juice (Morinda citrifolia L.) by spray drying Corrales García, Joel E.; Gallegos García, Flor Adriana; García Mateos, Ma. Rosario; Hernández Montes, Arturo; Ybarra Moncada, Ma. Carmen Universidad Autónoma de Chapingo, México [email protected] Noni isisa afruit fruit with with nutraceutical nutraceutical potential, potential, but its butunpleasant its unpleasant odor and odor flavor andlimit flavor its consumption. limit its consumption. Encapsulationis the process process to to entrap entrap active activeagents agentsinto intoparticles; particles;it itis isa apromissory promissory technology technologythat that may may allow mask bad taste and and bad bad smellcomponents. smellcomponents. The The aim aim was was to toassess assess the the nutraceutical nutraceutical potential potential innoni innoni juicemicroencapsulated by spray spray drying. drying. The The drying dryingtemperatures temperatureswere180 were180and and8585°C°Cof of inlet and outlet, inlet and outlet, respectively.Thecarrier materials materials (maltodextrine (maltodextrineDEDE-10,10, arabic gum, soy lecithin) were mixed in arabic gum, and and soy lecithin) were mixed in different proportions, proportions,searching searchingmaximum maximum bad-taste and bad-smell masking and minimum active bad-taste and bad-smell masking and minimum active compounds alteration. Noni juice was obtained from ripe, cleaned, and scaldedfruits(92 °C 55 min). min). A pulppulpremoving machine was used and then then the the juice juice was was obtained obtained by by centrifugation centrifugation of ofthe thepulp pulp(11000 (11000G, G,55°C °C 30 min). Phenols, Phenols, flavonoids, flavonoids, vitamin vitamin CC and and antioxidant antioxidantactivity activity(by (bythe theDPPH DPPHinhibition inhibitiontechnique), technique),drying drying efficiency and residual humiditywere measured. There was some important alteration of active compounds in different differentlevels levels (upon (upon the compound)by the compound)by high temperatures, high temperatures, however, however, the best treatment the best of treatment of microencapsulated noni noni juice juicewas waswith with 7070 % maltodextrin % maltodextrin + 29.9 + 29.9 % arabic % arabic gum + gum 0.1 % + 0.1 soy % lecithin soy lecithin (treatment 3) 3) totokeep keepthe themajor majorquantity quantity of of total total phenols, phenols, antioxidant antioxidant activity, activity, this treatment this treatment showedthe showedthe highest drying drying efficiency efficiency and andcaused causedthe the lowest lowest residual residual humidity. humidity. A high A high positive positive correlation correlation between between phenols and antioxidant activity was observed. The sensory sensory evaluation evaluation showed showed that that the the microencapsulated microencapsulated noni juice with treatment noni juice 3 waswith significantly treatmentless 3 was rejected significantly (22 %)less thanrejected the natural (22 juice %) than (46the %).natural juice Key words: phytochemicals, microencapsulated, spray drying, antioxidant activity, sensory evaluation 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-042 Phytochemical content of noni juice (Morinda citrifolia) microencapsulated in W/O/W emulsions Rodríguez Barahona, Carmen María; Corrales García, Joel E.; Hernández Montes, Arturo; Ybarra Moncada, Ma. Carmen Universidad Autónoma de Chapingo, México [email protected] Noni is a bush ripen have unpleasant and penetrating bush of ofAsiatic Asiaticorigin originwhich whichproduces producesfruits fruitsthat thatwhen whenget get ripen haveanan unpleasant and smell. The noni hasThe a lotnoni popularity because of itsbecause nutraceutical a healing properties, however, its unpleasant penetrating smell. has a lot popularity of its nutraceutical a healing properties, smell disfavors its consumption. The microencapsulation multiple emulsions is an for masking however, its unpleasant smell disfavors its consumption. by The microencapsulation byalternative multiple emulsions is smells and minimize the smells factorsandthat interfere secondary metabolites stability. The nutraceutical an alternative for masking minimize the in factors that interfere in secondary metabolites compounds noni juice compounds are sensibleoftononi highjuice temperatures, whichtemperatures, the objectiveforofwhich this study stability. The of nutraceutical are sensiblefor to high the is to microencapsulate noniisjuice by multiple emulsions disregarding theemulsions use of heat. To achieve the thisuse procedure objective of this study to microencapsulate noni juice by multiple disregarding of heat. juice noni To achieve was trapped this procedure in multiple noni emulsions, juice was using trapped as inwall multiple material emulsions, soy protein usingisolate as wall (SPI) material and Arabic soy protein(GA). gum isolate Phenolic, (SPI) andflavonoid Arabic gum and(GA). ascorbic Phenolic, acid flavonoid content and of three ascorbic emulsions acid content W/O/W of three with polymer emulsions W/O/W concentration (SPI-GA) with polymer of 6, 7.5concentration and 9 % were(SPI-GA) measured. of 6, The 7.5results and 9 showed % were measured. that did not The existresults significant showed that difference (Pdid < 0.05) not exist in phenolic significantand difference ascorbic(Pacid < 0.05) content in phenolic among and treatments. ascorbic The acid levels contentofamong flavonoids treatments. where higherThe in the levels treatments of flavonoids than the where oneshigher that existed in the treatments in the noni juice than control. the onesAs that conclusion existed inthe thepolymer noni juice control. content in theAs multiple conclusion emulsions the polymer did not content interfered in in thethe multiple phytochemical emulsionscontent did notamong interfered treatments. in the Key words: multiple emulsions, secondary metabolite, nutraceutical, noni juice 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-043 Antifungal activity of the root of Adenophyllum aurantium (L.) Strother Calderón Oropeza, Mónica Alicia; Sánchez Espinosa, Ana Claudia; Hernández Carlos, Beatriz; Cruz Vázquez, Julieta Karina; Cruz Cerón, Nallely Anali; Rojas Orizonte, Miriam Universidad del Mar, México [email protected] Medicinal plants plants research researchhas has made made available available substances substances for the fortreatment the treatment of differenthuman of differenthuman and and agricultural pests. pests. The The natural naturalproducts productsrepresent represent an an alternative alternative to synthetic to synthetic antifungal. antifungal. México México is theis the country with more species of Asteraceaein the the world, accounting accounting for for 12-15 12-15 % % of of México's México's vascular vascular flora. flora. ItIt has been reportedthat the the Asteraceae Asteraceae family family isisan animportant importantsource sourceofofpesticides.Specie pesticides.Speciethat that belongs belongsto to this family is Adenophyllumaurantium(L.) Strotherknown Strotherknown as as 'rudamontes' 'rudamontes' endemic endemic of ofMéxico. México.The Thepresenc e presence of bioactive of bioactive compounds compounds has been has demonstrated been demonstrated recently against recentlyroot against nematode root nematode Nacobbusaberrans and Nacobbusaberrans fungal pathogens Fusariumsolaniand and fungal pathogens Alternariaalternata. Fusariumsolaniand Hexane Alternariaalternata. and the ethyl acetate Hexane extract and of theroot ethyl were acetate extract prepared to determine of root were inhibition prepared of growth to determine mycelialin inhibition Fusariumoxysporum of growth mycelialin and Rhizoctoniasolani; Fusariumoxysporum the agar and Rhizoctoniasolani; diffusion assay, Petri dishes the agar with diffusion potato assay, dextrose Petri agar, dishes thiabendazole with potato dextrose (+) and ethanol agar, thiabendazole (-) controls was (+) and ethanol carried out. (-) Mycelial controls growth was carried rate of out. the Mycelial second, growth third and ratefourth of theday second, of incubation third and was fourth determined.Lik day of e preliminary result was found thatpreliminary fungi were result sensitive extracts, and it were wouldsensitive be interesting to identify incubation was determined.Like wastofound that fungi to extracts, and itthe substance responsible for this activity. Key words: adenophyllumaurantium, antifungal activity, growth rate 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-044 Characterization of policosanol from wax cochineal Martínez Ayala, Alma Leticia; Ramos Z., Emilia; Jiménez Aparicio, Antonio R. Centro de Investigación en Biotecnología Aplicada, Tlaxcala, México [email protected] Wax cochineal cochineal isis an anagrowaste agrowaste product productwhich whichcontains containsconsiderable considerableamount amount of of coccerina. coccerina. ThisThis hashas a a good potential for obtaining obtaining policosanol. policosanol. The The objetive objetive ofofthis thisstudy studywas wastotoexamine examinethe thealiphatic aliphatic alcohols alcohols content of of wax wax cochineal cochineal(Dactylopiuscoccus) (Dactylopiuscoccus)extracts extracts from from nymphs nymphs andand adultadult stages. stages. Extracts Extracts were were obtained by applying alkaline hydrolysis reaction. reaction. All All the the obtained obtained extracts extracts were were characterized characterized chemically chemically by Fourier Fourier Transform Transform Infrared Infrared Spectroscopy Spectroscopy(FTIR) (FTIR)in in thethe attenuated attenuated total total reflectance reflectance (ATR) (ATR) sampling sampling mode. For Forother otherside, side, thethe aliphatic aliphatic alcohols alcohols werewere identified identified and and quantified quantified by Gas by Chromatography Gas Chromatography coupled to to Mass Mass Spectrometric Spectrometric techniques techniques (GC-MS). (GC-MS). ATR-FTIR analysis indicate presence of aliphatic ATR-FTIRin analysis alcohols all the extracts, indicatewhich presence was of confirmed aliphatic by alcohols GC- MS. in all The thecomposition extracts, which of aliphatic was confirmed alcoholsbyofGCthe waxy extracts derived offrom cochineal wasofvery similar, being derived 1-triacontanol the most was abundant alcohol. MS. The composition aliphatic alcohols the waxy extracts from cochineal very similar, The content of was found The by the method 1 (5.24±0.36 g/kg), followed of method beinghighest 1-triacontanol the1-triacontanol most abundant alcohol. highest content of 1-triacontanol was found by the 2 (1.575±0.43 g/kg) y method 3 (0.520±0.03 g/kg) of wax cochineal adult stage. In wax method 1 (5.24±0.36 g/kg), followed of method 2 (1.575±0.43 g/kg) y from method 3 (0.520±0.03 g/kg)cochineal of wax cochinealfrom extracts from nymphs adult stage. stage,Inwas wax found cochineal likewise extracts by method from 1nymphs the higuest stage, content was found (1.66±0.41 likewise g/kg), by method followed 1 the of method higuest 2 content (0.515±0.05 (1.66±0.41 g/kg) yg/kg), method followed 3 (0.173±0.07 of method g/kg) 2 (0.515±0.05 (p<0.05). g/kg) Highy yield method of triacontanol 3 (0.173±0.07 was g/kg) (p<0.05). recuperated of cochineal wax in both insect stages. This component is important in the farmaceutical High yield of in triacontanol was recuperated cochineal waxcould in both stages.source This component is industry and the agriculture, by the whichofwax cochineal be insect an important of triacontanol. important in the farmaceutical industry and in the agriculture, by the which wax cochineal could be an Key words: policosanol, triacontanol, cochineal, wax, insect 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-045 Use of Citrus lemon L. (lemon) in the treatment of rises of arterial pressure Avello Lorca, Marcia Andrea ;Jofré, P.; Fernández Pastene, E. University of Concepción, Chile [email protected] Introducción. La Lahipertensión hipertensiónarterial arterial es es una una enfermedad enfermedad crónica, crónica, de etiología de etiología multifactorial multifactorial y que y que corresponde aa lalaelevación elevaciónpersistente persistentedede la presión la presión arterial arterial sobre sobre los límites los límites referenciales. referenciales. EntreEntre los los problemas de salud salud aa los los que que puede puede verse verseenfrentado enfrentado elelpaciente pacientehipertenso, hipertenso,destacan destacanlas lasalzas alzasdede presión presión arterial. arterial. Aquí, el Aquí, paciente el paciente puede optar puedepor optar acudir por aacudir algúnasistema algún sistema públicopúblico de urgencia de urgencia o realizar o realizar medidas medidas de autoatención. de autoatención. A nivel popular A nively popular en diferentes y en diferentes fuentes defuentes información de información se menciona se menciona que el zumo quedeelCitrus zumo de Citrus limon L. Burm. f.(Rutaceae)es útil para disminuir la presión arterial. Sin embargo, limon L. Burm. f.(Rutaceae)es útil para disminuir la presión arterial. Sin embargo, este uso no este tiene un uso no tiene un respaldo científico del todo El claro. respaldo científico del todo claro. Objetivo. objetivo del trabajo fue analizar el uso del zumo de C. limon, Objetivo. en el manejo El objetivo del alza delde trabajo presión fue arterial. analizar Metodología:Para el uso del zumo deesto C. limon, se analizó en el la manejo composición del alza química de presión de arterial.de C. limón disponibles en la ciudad de Concepción, Chile, a través de métodos espectroscópicos y frutos Metodología:Para Para cromatográficos. esto caracterizar se analizó laelcomposición uso del zumo, química se aplicó de frutos una encuesta de C. limón a 506 disponibles pacientes enhipertensos la ciudad de Concepción, del CESFAM Dr.Chile, VíctoraManuel través de Fernández métodos de espectroscópicos la ciudad de Concepción, y cromatográficos. y se realizó Parauncaracterizar estudio piloto el uso con delpacientes 5 zumo, sehipertensos, aplicó una encuesta para evaluar a 506 efecto pacientes del zumo hipertensos frente undel alza CESFAM de presión Dr.arterial. Víctor Manuel Resultados:Como Fernández se resultados de identificaron la ciudad de polifenoles Concepción,presentes y se realizó en un el zumo estudio depiloto C. limon con como 5 pacientes los flavonoides hipertensos, eriocitrina, para evaluar efecto hesperidina y diosmina. del zumo frente Con respecto un alza de a los presión datosarterial. compilados de la encuesta, el 95.2% de la población Resultados:Como encuestada ha usado resultados alguna se vezidentificaron zumo de C.polifenoles limon antepresentes un alza de enpresión el zumoarterial, de C. limon con una como mejoría los de flavonoides los síntomaseriocitrina, aproximadamente hesperidina en ymedia diosmina. hora.Con Enrespecto el estudio a los piloto datos secompilados observó una de disminución la encuesta, de el la 95.2% de presión arterial la población en hipertensos encuestada en situación ha usadodealguna alza devez presión, zumo de queC.tiende ser sostenida el tiempo, no limonaante un alza deen presión arterial, así en pacientes con una mejoría normotensos. de los síntomas Discusiónaproximadamente y Conclusión. Elen uso media del zumo hora. En de el C.estudio limon frente piloto un se alza de observó arterial, presión una disminución está ampliamente de la presión arraigado arterialenenlahipertensos cultura popular en situación y se concluyó de alza que dedisminuye presión, que la presión tiende a ser sostenida arterial por un mecanismo en el tiempo,denoacción así en no pacientes establecido, normotensos. probablemente relacionado con el contenido en Discusión yyConclusión. El uso del zumo C. limon frente alza de efectos presión antihipertensivos. arterial, está ampliamente polifenoles vitamina C, compuestos a losdecuales se les hanun atribuido arraigado en la cultura popular y se concluyó que disminuye la presión arterial por un mecanismo de acción no establecido, probablemente relacionado con el contenido en polifenoles y vitamina C, Key words: Citrus lemon L., treatment, arterial pressure 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-046 Evaluation of the hypoglycemic effect of Bauhinia forficata in patients with poorly controlled diabetes Córdova Mariángel, Pía; Avello Lorca, Marcia Andrea; Fernández, P.; Villa, L. University of Concepción, Chile [email protected] Introduction. Diabetes Diabetes mellitus mellitusisisa achronic chronic disease become increasingly prevalent in recent disease thatthat has has become increasingly prevalent in recent decades. The The objective objective of oftreatment treatmentisistotoreduce reducecardiovascular cardiovascularrisk. risk. The The useuse of medicinal of medicinal plants plants in the in the treatment of chronic diseases has has attracted attracted scientific scientific interest interest ininrecent recentyears. years.InInChile, Chile,a acommon commonform formofof alternative medicine for for diabetes diabetes is is the theleaf leafofofthe theBauhinia Bauhiniaforficata forficata("Pata ("PatadedeVaca") Vaca")plant. plant. Objective. To evaluate the hypoglycemic effect of B. forficata leaves, administered as complementary therapy for patients Objective. with poorly To controlled evaluatetype-2 the hypoglycemic diabetes mellitus. effect Methodology. of B. forficata leaves, A semi-experimental administered study as complementary was conducted on therapy for Chilean patients patients in awith primary poorly healthcare controlledsetting type-2who diabetes have poorly mellitus. controlled type-2 diabetes mellitus (HbA 1C >7%). These patients were administered a 0.4% infusion of B. forficata leaves, twice daily for a period of Methodology. three months, Aand semi-experimental then a period ofstudy one month was conducted without infusion. on Chilean Clinical patients parameters in a primary such healthcare as body mass setting(BMI), index who have bloodpoorly pressure controlled and blood type-2 glucose diabetes weremellitus measured (HbA1C every>7%). month. These Total patients cholesterol, weretriglycerides administered and glycated hemoglobin a 0.4% infusion (HbA1C) of B. forficata were measured leaves, twice every daily threeformonths. a periodResults. of threeFor months, patients and receiving then a the period ofofone infusion B. forficata month without leaves,infusion. a decrease Clinical in HbA1C parameters levelssuch of 0.5% as body wasmass observed. index No (BMI), significant blood pressure changes and in weight, blood blood glucose pressure, were measured total cholesterol, every month. triglyceride Total cholesterol, and glucosetriglycerides levels were and observed. glycated Discussion hemoglobin and (HbA1C) were Conclusion. Themeasured infusion of every B.forficata three months. leaves, when used as an adjunct to pharmacological treatment along with a healthy lifestyle, may decrease the levels of HbA1C in the adult diabetic population. Results. For patients receiving the infusion of B. forficata leaves, a decrease in HbA1C levels of 0.5% was observed. No significant changes in weight, blood pressure, total cholesterol, triglyceride and glucose levels were observed. Discussion and Conclusion. The infusion of B.forficata leaves, when used as an adjunct to pharmacological treatment along with a healthy lifestyle, may decrease the levels of HbA1C in the adult Key words: hypoglycemic effect, Bauhinia forficata, patient, diabetes 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-047 In vitro Anthelmintic Efficacy of Aloe ferox, Agave sisalana and Gunnera perpensa Against Heterakis gallinarum Manjoro Mwale, Marizvikuru Institute for Rural Development, University of Venda, South Africa [email protected]; [email protected] Heterakis gallinarum gallinarum impedes impedesthethe productivity productivity of village of village chickens chickens and hence and their hence socio-economic their socio-economic contribution to to rural rural livelihoods. livelihoods.Smallholder Smallholderfarmers farmersareare endowed endowed withwith vastvast indigenous indigenous knowledge knowledge for for controlling H. gallinarum and and they they predominantly predominantly use useAloe Aloeferox, ferox,Agave Agavesisalana sisalanaand andGunnera Gunneraperpensa. perpensa. However, their their anti-helminthic anti-helminthic efficacy efficacy isisunknown. unknown.Therefore, Therefore,the the study study objective objective waswas to determine to determine the the anthelmintic efficacy efficacy ofofA.A.ferox, ferox, A. A. sisalana sisalana andand G. perpensa G. perpensa against against H. gallinarum H. gallinarum in vitro.inTen vitro. H. Ten H. gallinarum worms were randomly introduced introduced into into each each of of the the 42 42 petri-dishes. petri-dishes. InIn triplicate, triplicate, treatments treatments were were positive control (distilled water), negative control (mebendazole) and A. ferox, A. sisalana and G. G. perpens a perpensa leaf aqueous aqueous extracts leafwith extracts doseswith 7.25, doses 14.5,7.25, 29 and 14.5, 58 29 mg/mL. and 58 Worm mg/mL. motility Worm inhibition motilityand inhibition worm and recovery wormnoted was recovery over was 60 h.noted Data over was 60 analysed h. Datausing was analysed general linear usingmodel general of Statistical linear model Analyses of Statistical System. Agave Analyses(14.5 sisalana System. mg/mL) Agave hadsisalana highest (14.5 (80%)mg/mL) worm motility had highest inhibition (80%) at 12-h worm interval motility (p<0.05) inhibition and at highest 12-h worm interval (p<0.05) mortality index (80%) and highest showingworm that the mortality plant has index anthelmintic (80%) showing properties. that the Worms plant(70%) has anthelmintic were recovered after 48 h following treatment ferox (58 after mg/mL) 50% for G. perpensa allferox dose(58 levels. Thisand signifies properties. Worms (70%)with wereA.recovered 48 hand following treatment with A. mg/mL) that hasall moderate anthelmintic activities while those of A. moderate ferox are anthelmintic primarily ascribed to its 50% G. for perpensa G. perpensa dose levels. This signifies that G. perpensa has activities purgative effects. Agave mg/mL) was the most efficacious reducing motility and was causing while those of A. ferox aresisalana primarily(14.5 ascribed to its purgative effects. Agave insisalana (14.5 mg/mL) mortality H. gallinarum in vitro. motility Findings arecausing useful to resource-constrained selecting best the most of efficacious in reducing and mortality of H. gallinarumfarmers in vitro. for Findings arethe useful to resource-constrained plant and pharmaceutics farmers for manufacturing for selecting anthelmintic the best plant drugs. and pharmaceutics for manufacturing Key words: chickens, heterakis gallinarum, plant leaf extracts, worm mortality, worm motility 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-048 Immune modulatory effect of Korean native chicken meat Kang Hyun, Leem; Myung Gyou, Kim; Hye Kyung, Kim Semyung University, South Korea [email protected] Introduction: Chicken essence essence has has been beenwell wellknown knownforforitsits health-promoting health-promotingand and enhancement enhancement of body of body immunity. However, the mechanism underlying underlying its its effect effect remains remains unclear. unclear. This This study studyaims aimstotoexamine examinethe the immune-modulatory effect effect ofoffour four different different Korean Korean nativenative chickens chickens (Yeonsan (Yeonsan Ogye, Hyunin Ogye, Hyunin Black, Black, Hwangbong, and Hoengseong Yakdak) on macrophages. White Leghorn was was used used as as aa control. control. Materials Materials and Methods: and Methods: Breast and Breast leg meat and leg were meat grounded were grounded and extracted and extracted with 70%with ethanol 70% at ethanol 60 oCatfor 606oC hours. for 6 hours. The proliferation The proliferation of macrophage of macrophage like RAW264.7 like RAW264.7 cells wascells assayed was assayed by a colorimetric by a colorimetric immunoassay, which immunoassay, is based on quantitatingbromodeoxyuridine which is based on quantitatingbromodeoxyuridine (BrdU) incorporation(BrdU) into the incorporation newlysynthesized into the DNA. The newlysynthesized uptake of fluorescein-labeled DNA. The uptake Escherichia of fluorescein-labeled coli was measured Escherichia to determine coli was measured whether chicken to determine extract whether chicken stimulates the phagocytic extract stimulates activity ofthe RAW264.7 phagocytic cells. activity The of quantity RAW264.7 of nitric cells. oxide The(NO) quantity was of determined nitric oxideby (NO) was Griess assay determined in LPS-stimulated by Griess RAW assay264.7 in LPS-stimulated cells. Results: RAW All chicken 264.7 cells. strains of breast meat extracts(50Results: 200 ug/mL) All chicken increased strains cell proliferations of breast meat while extracts(50-200 leg meats fail to ug/mL) affect increased cell proliferations. cell proliferations Breast meat while extracts leg meatsug/mL) fail to affect proliferations. extracts (200Black ug/mL) two strains ofYakdak) Korean enhanc native ed (200 of twocell strains of KoreanBreast native meat chicken (Hyunin andofHoengseong chicken the phagocytic (Hyuninability. BlackFurthermore, and Hoengseong LPS-Yakdak) stimulated enhanced release the of nitric phagocytic oxide was ability. significantly Furthermore, decreased LPS- by stimulated breast meats release of fourof Korean nitric oxide native was chicken significantly strainsin decreased dose-dependent by breastmanner meats of while four breast Koreanmeat native of White chicken strainsin Leghorn fail to affect dose-dependent nitric oxide release. manner Nitric while oxide breast production meat of was White notLeghorn affectedfail by to legaffect meats. nitric Conclusion: oxide release.results These Nitric oxide suggests production that Korean was notnative affected chicken by legmeat, meats.especially breast meat, stimulates immune Conclusion: functions of macrophages. These results suggests that Korean native chicken meat, especially breast meat, stimulates Key words: korean native chicken, raw 264.7 cells,nitric oxide, phagocyte activity, immune function 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-049 Concentrations of Se as an essential metal in human milk of venezolan women Angulo Mavo, Andrea María; Chávez, Mervin; Granadillo Morán, Víctor Alberto Facultad Experimental de Ciencias, Venezuela [email protected] Human milk is a biological biological fluid, fluid, which whichprovides provides all allthe thenutrients, nutrients,including includingessential essentialmetals metalsthat thatare arerequired required for the development for the development and growth and growth of newborn of newborn children children duringduring the first the first months months of life; of life; also, also, it itcontains contains enzymes, enzymes, specific proteins, specific proteins, nucleotides, nucleotides, etc. Se concentration etc. Se concentration in milk is in directly milk is directly affectedaffected by levels by in the levels chain, food in the also food chain, is a component also is a component of two enzymes, of two enzymes, glutioneperoxidase glutioneperoxidase thats protects thats protects against against oxidative oxidative damage, damage, and of type and I ofiodothyronine type I iodothyronine 5-de-iodinase, 5-de-iodinase, this antioxidative this antioxidative cellularcellular Se might Se might have have a role a in role in preventing preventing Cancer.Cancer. As essential As essential nutrients, nutrients, many chemicals many chemicals can be can transferred be transferred from body fromstorage body storage sites and sitesblood the and the of nursing blood ofmothers nursing mothers to breasttomilk. breast Nowadays, milk. Nowadays, researchs researchs on the importance on the importance of essential of trace essential metals in trace human metals milk, in itshuman nutritional milk,quality its nutritional and its relation quality and withitsenvironmental relation with environmental factors are growing. factors Inare this growing. work, it isInpresented this work,the it istotal presented concentrations the total of concentrations Se in samplesofinSehuman in samples milk using in human ICP- milk MS. using The samples ICPMS. The were collected samples fromwere breastfeeding collected from women breastfeeding between 2 women and 8 post-partum between 2 and months 8 post-partum in the Hospital months of Children in the of VeritasofinChildren Maracaibo city. For of organic material, digestion the real digestion samples with HNO3 Hospital of Veritas in destruction Maracaibo city. For destruction of organicofmaterial, of the real / samplesboth H2O2., with concentrated, HNO3 / H2O2., was both applied. concentrated, The totalwas metal applied. concentration The totalwas metal 0,011 concentration mg L-1 of was Se. 0,011 The limit mgdetection of L-1 of Se. (LD=3?/m) The limit of obtained detection was(LD=3?/m) 0,003 for Se. obtained The precision was 0,003 (expressed for Se. The as precision RDS) was(expressed < 5%. Accuracy as RDS)assessed was was < 5%.byAccuracy recoverywas studies, assessed obtaining by recovery mean studies, recoveries obtaining percentages mean recoveries of 100 ± 5%. percentages Developed of 100 ± 5%.forDeveloped methods the determination methods of fortotal the determination concentration of total the studied concentration metal was of the accuracy, studied metal precise was and free from interferences. Key words: human milk, essential metal, icp-ms 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-050 Chemical composition of Salvia aratocensis extract obtained by supercritical fluid extraction and its antigenotoxicity against ultraviolet radiationinduced DNA damage Fuentes Lorenzo, Jorge Luis; Stashenko, Elena E.; Quintero Ruiz, Nathalia Colombia [email protected]; [email protected] Introduction: Salviais Salviais aacosmopolitan cosmopolitanand and important important genus, genus, widely widely usedused in flavoring in flavoring and traditional and traditional The present work evaluated the the medicine for for treatments treatments ofofrespiratory respiratoryand andgastrointestinal gastrointestinaldiseases. diseases. The present work evaluated chemical composition of Salvia aratocensis extract extract obtained obtained by by CO2 CO2 supercritical supercritical fluid fluid extraction,as extraction,as well well as its itsDNA DNAprotective protective effect effect against against ultraviolet ultraviolet radiation radiation (UVR)-induced (UVR)-induced genotoxicity. genotoxicity. Material and Material andaratocensis Methods:S. Methods:S.extract aratocensis composition extract was composition determined wasbydetermined gas chromatography-mass by gas chromatography-mass spectrometry spectrometry (GC-MS). The(GC-MS). antigenotoxic The antigenotoxic effect of S. aratocensis effect of S. aratocensis extract was extract assayed was in assayed co-incubation in co-incubation procedures procedures using the SOS using chromotest the SOS in chromotest Escherichia in coli Escherichia PQ37 cells. coliResults PQ37 cells. and Discussion: The major compounds Results of the plant and extract Discussion: were The ?-cadinol major (31.8%), compounds 1,10-epiof the cubenol plant extract (12.9%), were trans-?-caryophyllene ?-cadinol (31.8%), 1,10-epi(7.7%), cubenol (12.9%), cadinene (6.6%), trans-?-caryophyllene and squalene (6.5%).(7.7%), S. aratocensis -cadineneextract (6.6%), protected and squalene bacterial (6.5%). cells S. against aratocensis UVRextract protected induced genotoxicity bacterial at doses cellsbetween against 0.063 UVR-induced and 1 mg/mL. genotoxicity The results at doses werebetween discussed 0.063 in relation and 1 mg/mL. to the The results DNA protective werepotential discussed of the in relation S. aratocensis to the DNA extract protective and its potential major constituents. of the S. aratocensis extract and its Key words: salvia aratocensis, supercritical fluid extraction, antigenotoxicity, sos chromotest, ultraviolet 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-051 Lippia origanoides extract obtained by supercritical fluid extraction and its major constituent pinocembrin diminish ultraviolet radiation-induced DNA damage and modulate cell division in Escherichia coli Fuentes L., Jorge Luis; Rey Castellanos, Natalia; Estévez Castro, Carlos Felipe; Stashenko, Elena E. Colombia [email protected]; [email protected] Introduction: Lippia origanoides is is an an aromatic aromatic shrub shrub native native totoCentral Centraland andSouth SouthAmerica. America.The Theplant plantisisused used as seasoning as seasoning and in andtraditional in traditional medicine medicine for for treatment treatment of gastrointestinal, of gastrointestinal, genitourinary genitourinaryand andrespiratory respiratoryasdiseases diseases well as as anti-inflammatory well as anti-inflammatory and analgesic and analgesic remedy.The remedy.The present work present evaluated work evaluated the chemical the chemical composition composition of L. origanoides of L. origanoides extract obtained extract obtained by CO2 supercritical by CO2 supercritical fluid extraction, fluid extraction, and its antigenotoxic and its antigenotoxic activity againstactivity UVR-induced against UVR-induced genotoxicity. Major genotoxicity. constituent Major of constituent this extract,ofpinocembrin, this extract, was pinocembrin, also studied. was also and Material studied. Methods:L. origanoides extract composition was determined by gas chromatography-mas s spectrometry (GC-MS). The antigenotoxic effect of L. origanoides extract was assayed in co-incubation Material andusing procedures Methods:L. the SOS origanoides chromotest extract in Escherichia composition coliwas PQ37 determined cells. Fluorescence by gas chromatography-mass microscopy analysis spectrometry was used in cell (GC-MS). treatments The in antigenotoxic order to determine effect ofinterferences L. origanoideswith extract cell division; was assayed different in co-incubation nucleoid shapes procedures were categories usingmeasured. the SOS chromotest Results and in Escherichia Discussion: coli ThePQ37 principal cells. constituents Fluorescence of the microscopy plant extract analysis were was used in cell pinocembrin (54.9%), treatments trans-?in order caryophyllene to determine (10.9%), interferences carvacrolwith (6.3%), cell division; p-cimene different (6.2%), nucleoid thymolshapes (4.6%), categories and 1,8-cineole were (3.5%). measured. L. origanoidesextract protected bacterial cells against UVR-induced genotoxicity at doses between 0.016 and 1 mg/mL; this effect was related to its major constituent pinocembrin. Results and Discussion: Fluorescence microscopyThe analysis principal showed constituents that pinocembrin of the plant interfered extract with wereE.pinocembrin coli cell division, (54.9%), whattrans-?provide caryophyllene first evidence on (10.9%), bioantimutagenic carvacrol (6.3%), potential p-cimene of the pinocembrin.The (6.2%), thymol (4.6%), results were and 1,8-cineole discussed in (3.5%). relation L. to the origanoidesextract protected bacterial cells against UVR-induced at doses between 0.016 DNA protective potential of the L. origanoides extract and its majorgenotoxicity constituents. and 1 mg/mL; this effect was related to its major constituent pinocembrin.Fluorescence microscopy analysis showed that pinocembrin interfered with E. coli cell division, what provide first evidence on Key words: antigenotoxicity, sos chromotest, ultraviolet radiation, lippiaoriganoides, supercritical fluid 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-052 Chemical composition of essential oil and extract obtained by supercritical fluid extraction from Turnera diffusa and its antigenotoxicity against UVRinduced DNA damage Fuentes Lorenzo, Jorge Luis; Stashenko, Elena E.; Cely Coronado, Lady Johanna; Cristancho Gómez, Rowens Andreick; Quintero Ruiz, Nathalia Colombia [email protected]; [email protected] Introduction: Turnera Turnera diffusa, diffusa, commonly commonlyknown knownasasdamiana, damiana, is ais shrublargely a shrublargely used used as aphrodisiacs as aphrodisiacs in in American traditional medicine, medicine, but but also alsofor for treatments treatmentsofofskin, skin,respiratory respiratoryand andgastrointestinal gastrointestinaldiseases. diseases.The The present present workwork evaluated evaluated the chemical the chemical composition composition of T.of diffusa T. diffusa essential essential oil (EO) oil (EO) andand extract extract obtained by CO2 supercritical fluid extraction as, itswell DNA effect against radiation obtained by CO2 supercritical fluid(SFE),as extractionwell (SFE),as as,protective its DNA protective effectultraviolet against ultraviolet radiation (UVR)-induced (UVR)-induced genotoxicity. genotoxicity. Material and Methods: T. diffusa extracts compositions were determined by gas chromatography-mass spectrometry (GC-MS). The antigenotoxic effect of T. diffusa EO and extract Material were assayed and Methods: in co- incubation T. diffusa extracts procedures compositions using thewere SOSdetermined chromotestbyingas Escherichia chromatography-mass coli PQ37 cells. Results and Discussion:The major constituents diffusa drima-7,9(11)-diene (28%) and spectrometry (GC-MS). The antigenotoxic effect ofinT.T.diffusa EOEO andwere extract were assayed in codihydro-karanone (25%); in the obtained by mostly coli 3-deoxy-isopetasol (64%) and 4,5-di-epiincubation procedures using the extract SOS chromotest in SFE Escherichia PQ37 cells. aristolochene (22%), were detected. Both T. diffusa EO and extract obtained by SFE protected bacterial cells against UVR-induced genotoxicity in a dose-dependent These results were discussed in Results and Discussion:The major constituents in T. diffusa EO manner. were drima-7,9(11)-diene (28%) and relation to the DNA(25%); protective of the T.by diffusa EO and3-deoxy-isopetasol extract obtained by SFEand and4,5-di-epitheir major dihydro-karanone in thepotential extract obtained SFE mostly (64%) aristolochene constituents. (22%), were detected. Both T. diffusa EO and extract obtained by SFE protected bacterial cells against UVR-induced genotoxicity in a dose-dependent manner. These results were discussed in relation to the DNA protective potential of the T. diffusa EO and extract obtained by SFE and their major Key words: turneradiffusa, essential oil, supercritical fluid extraction, antigenotoxicity, sos chromotest, 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-053 The seed of oleaginous extract of Carapa guianensis aublet a potent antioxidant does not damage the DNA in experila models Arencibia Arrebola, Daniel Francisco; Narciandi, Jessica; Rosario, Luis Alfredo; Delgado, Livan; Vidal Novoa, Alexis Instituto Finlay, Cuba [email protected] The majority presence of of polyunsaturated polyunsaturated fatty fatty acids acids and andphenolic phenolic compounds compounds ininseed seedoiloilextract extractofofCarapa Carapa guianensis guianensis Aublet has Aublet great has potential great potential as antioxidant. as antioxidant. But the extract But theeffect extract oneffect DNA on it isDNA ignored.The it is aim of ignored.The this work wasaim evaluated of this work the mutagenic was evaluated and genotoxic the mutagenic potential and genotoxic of the extract.The potentialstudies of the extract.The were performed in vitro (Ames test) with and without addition theand microsomal hepaticoffraction, in vivo assays thatfraction, included studies were performed in vitro (Ames test) of with without addition the microsomal hepatic on peripheral leukocytes of Sprague-Dawley rats using the comet assay,rats the using micronucleus assays in vivo assaysblood that included on peripheral blood leukocytes of Sprague-Dawley the comet and chromosomal aberrations in BALB/c mice of both sexes administered 14both dayssexes orally in repeated assay, the micronucleus assays and chromosomal aberrations in BALB/cduring mice of administered doses (400, 1000 duringand 14 days 2000 orally mg/kg) in repeated and testing doses of the (400, sperm 1000head and 2000 morphology mg/kg) and of male testing BALB/c of the mice sperm head morphology administered during 35 days of male withBALB/c doses mice of 400, administered 1000 and during 2000 mg/kg. 35 daysInwith bacteria, doses the of 400, extract 1000did and not 2000 mg/kg. cytotoxic and In mutagenic. bacteria, In thethe extract in vivodidassays, not cytotoxic there was and not mutagenic. increaseInthe theformation in vivo assays, of alkalithere labilewas sites notin increase DNA of the SD formation rats leukocytes of alkaliand labilethere sites was in DNA not ofincrease SD rats leukocytes the frequency and there of bone was marrow not increase cells the with frequency of bone micronucleus and cells marrow withcells chromosomal with micronucleus aberrations and(structural cells with and chromosomal numerical). aberrations aberrations It did not increase (structural (structural in and the and numerical). It did spontaneous frequency not increase of anomalies in the the spontaneous spontaneous in the spermfrequency frequency head morphology; of anomalies anomalies however, thesperm the sperm results headof morphology the sperm ; not in of ininthe head morphology; concentration in the of treated groupconcentration with 2000 mg/kg the extract were2000 different with the extract negative and however, the results the sperm in the of treated group with mg/kg of the were positive control groups. The results indicate that this extract is not mutagenic or genotoxic according to the performed assays. Key words: carapa guianensis, genotoxicity assay, antioxidant 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-054 Assessment of antioxidant effect of seed oleaginous extract Carapa guioanensis aublet using in vivo models Curveco, Dayisell Lázara; Arencibia Arrebola, Daniel Francisco; Rosario, Luis Alfredo; Alonso, Adriana; Delgado, Livan; Vidal Novoa, Alexis Instituto Finlay, Cuba [email protected] The seed oil oil extract extractofofCarapa Carapaguianensis guianensisAublet Aublet is is a natural a natural product product constituted constituted by abycomplex a complex mixture mixture among whose components were the poli unsaturated unsaturated fatty acids and and terpenoids. terpenoids. The The aim aim of of this this work work was was evaluated the potential potential of ofthe theextract extractlike likeantioxidant antioxidantnutritional nutritionalsupplement supplementin in Sprague-Dawley Sprague-Dawleyratsrats withwith future potential as antimutagenic supplements useful in support of antineoplastic therapy. Different dose of the extract extract 100, 100, 200 200and and400 400mg/kg mg/kgwere wereadministered administered in co-treatment in co-treatment withwith Cyclophosphamide Cyclophosphamide (CP)(CP) a a potent inductor of of oxidative oxidative stress stressand andititwas wasalso alsoadministered administeredthe themaximum maximumdose doseofof the the product product 400 400 mg/kg. mg/kg. In all the In cases all the were casesdetermined were determined the influence the influence of these of treatments these treatments in the in water the water and foods and foods intake,intake, corporal corporal weight, haematology and biochemistry sanguine the plasmatic antioxidant The weight, haematology and biochemistry sanguine test andtest theand plasmatic antioxidant state.state. The obtained obtaineddemonstrated results results demonstrated that the consumption that the consumption of the extract of the in co-treatment extract in co-treatment with the CP with didthe notCP affect did the not water affectfoods and the water intake andneither foods intake the increase neither the the increase corporal the weight corporal when weight 200 when mg/kg 200 doses mg/kgwere doses used. were The used. The haematological haematological and biochemical and biochemical parametersparameters analyzed were analyzed not affected were notfor affected none of forthe none evaluated of the doses, evaluated being abledoses, to correct being theable hepatotoxicity to correct the caused hepatotoxicity by the drug caused and the by the disorders drug and in the the lipid disorders metabolism. in the lipid The metabolism. seed oleaginous The seed extract oleaginous of Carapaextract guianensis of Carapa Aublet guianensis was a good Aublet antioxidant was a good that antioxidant which demonstrated that whichin demonstrated the reported values in theofreported enzymatic values activity, of enzymatic of the main activity, biomarker of theofmain oxidative biomarker stressofused. oxidative With stress these results, used. we thewebeginning of the studies toxicology studies) and thestudies) preclinical Withrecommend these results, recommend the security beginning of the (preclinical security studies (preclinical toxicology antimutagenic studies of the extract useful during the antineoplastic therapy. Key words: carapa guianensis aublet, antioxidant, in vivo, antimutagenesis, cyclophosphamide 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-055 Pharmacognostical study of Schinus terenbinthifolius Raddi (false copal). Tkalenko CA. Águila Tkalenko, Clara; Álvares Hernández, Yarelys; Mena Álvarez, Odaimi; Milanés Fernández, Marlen Empresa de Productos Inyectables LABIOFAM, Cuba [email protected] The extracts extracts hydro-alcoholic hydro-alcoholic obtained obtainedstarting startingfrom fromthethe leaves leaves of Schinus of Schinus terebinthifolius terebinthifolius Raddi Raddi (false(false copal), are are broadly broadly used usedbybytheir their anti-inflammatory anti-inflammatory andand antimicrobial antimicrobial properties. properties. Our Our objective objective was was to to develop a farmacognóstico study study of of Schinus Schinus terebinthifolius terebinthifolius Raddi Raddi(false (falsecopal). copal).Taxonomicthe Taxonomicthebotanical botanical species was was classified classifiedand andit it evaluatedthethe morphology of leaves the leaves visually. The drying study was evaluated morphology of the visually. The drying study was developed under different conditions being being measured measured humidity, humidity, drying drying temperature temperature and and loss lossof ofweight. weight.The vegetable dry material was was stored in flask of glass amber, bags bags of paper kraft kraft and polyethylene bags bags being The vegetable dry material stored in flask of glass amber, of paper and polyethylene being evaluated evaluated humidity, humidity, characteristic characteristic organoleptic organoleptic and microbial and microbial load. Starting load. Starting from anfrom extract an extract hydro- hydroalcoholic alcoholic to 80% obtained to 80% by obtained maceration by maceration secondarysecondary metabolites metabolites were identified were identified using the using technique the technique of Rondinaofand Rondina and Coussio (1969) Coussio modified (1969) by modified Durand (1986). by Durand To (1986). this extract To this they extract werethey determined were determined physical-physicalchemical chemical parameters: parameters: characteristic characteristic organoleptic, organoleptic, pH, relative pH, density, relative density, and refraction and refraction index, alcoholic index, alcoholic content and content total solids. and Metabolites total solids. Metabolites of interest were of interest identified wereby identified chromatographic by chromatographic in thin layer. in thin Total layer. phenols Total were phenols were quantified by visible quantified UV to by765 visible nm.UV Thetoresults 765 nm. threw The that results it was threw about thatthe it was species about Schinus the species terebinthifolius Schinus terebinthifolius Raddi in correspondence Raddi in correspondence with the consulted with the bibliography. consulted The bibliography. solar dryer Theand solar thedryer nylonand bags thewere nylonthe bags were most efficient the methods most efficient for the methods obtaining for the andobtaining storage and of the storage vegetable of thedry vegetable material. drySecondary material. several Secondary several metabolites was identified metabolites in the wasextract identified hydro-alcoholic in the extract(pH: hydro-alcoholic 5.30, density: (pH:0.8902, 5.30, density: total solids: 0.8902, 8.4%, refraction index: alcoholic content:alcoholic 72%), steroids, alkaloids,resins, phenols and tannins being total solids: 8.4%,1.3786, refraction index: 1.3786, content:resins, 72%), steroids, alkaloids, phenols obtained, a 7.88% of total phenols agreeing with that obtained by other authors. Key words: schinus terebinthifolius raddi, farmacognóstico, physical-chemical parameters 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-056 Extraction of flavonoids from leaves of Annona muricata L. Henry García, Yaima; Salomón Izquierdo, Suslebys; Acosta Esquijarrosa, Jhoanny; Romero Díaz, Aylema; López de Armas, Marilyn; Mercado Vidal, Josué EPVVB, Grupo Empresarial LABIOFAM, Cuba [email protected] The soursop (Annona muricata L) L) is used used in in the the ethno-medicine ethno-medicine for for the thetreatment treatmentof ofdifferent different diseases. diseases.The The presence presence of flavonoids of flavonoids in the in leaves the leaves havehave beenbeen determined determined which which havehave antidiabetic, antidiabetic, antioxidant antioxidant andand antianti-inflammatory properties. study to evaluate influence of operational parameters inflammatory properties. The The study aimsaims to evaluate the the influence of operational parameters in in obtaining obtaining extraction extraction of flavonoids of flavonoids from the leaves from the of leaves Annonaofmuricata Annona muricata L . Materials L . Materials and Methods: and Methods: The evaluation The of evaluation the operating of the variables operating was variables performed was using performed an experimental using an experimental design ofdesign response of response surface surface rotational rotational comprised comprised central 2 central ^ 3 with2 star ^ 3 with points star, points being selected , being selected the intervals the intervals between between 50 and5070mL and 70mL /g for /g the for the relation relation vegetal-volume vegetal-volume materialmaterial of solvent of solvent , between , between 40 and40 80% andthe 80% ethanol the ethanol concentration concentration and between and between 2 and 3 h2the andextraction 3 h the extraction time. The time. quantification The quantification of total flavonoids of total flavonoids was performed was performed by a colorimetric by a method colorimetric at 430 nm, expressed method at 430 as quercetin. nm, expressed Results as and quercetin. Discussion: Results theand presence Discussion: of flavonoids the presence and quercetin of in flavonoids the extracts andwas quercetin determined. in the extracts The significant was determined. variables The resulted significant the quadratic variables effects resultedofthe plant-material quadratic effects of plant-material relationship solvent volume relationship and thesolvent concentration volume and of ethanol. the concentration The optimum of ethanol. extraction The condition optimum in total extraction condition flavonoids was obtained in total at a flavonoids concentration was obtained of ethanolatofa 96%, concentration a ratio of70 of ethanol mL/ g and of 96%, a timea of ratio 1.6h, of70 to mL/ yield g andprocess. 87% a time ofTo1.6h, the best to yield experimental 87% process. condition To thetriplicate best experimental experimentscondition were performed triplicate and experiments comparedwere with performed the value predicted and compared by thewith design, the value a good predicted correlation by thewas design, founda good between correlation them. Conclusions: was found between The best extraction conditionsThe were selected in obtaining extract from the leaves of with the presence them. Conclusions: best extraction conditionsanwere selected in obtaining ansoursop extract from leaves of flavonoids. Key words: annona muricata l, flavonoids, solid-liquid extraction 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-057 Study in silico of the DNA protective effects of the 2,6 –disecbutilphenol extracted from the plant Phyllanthus orbicularis K Castanedo Monteserín, Lázaro Andrés ; Marrero Gutiérrez , J.; Lamar Sánchez, A. Universidad de La Habana (UH), Cuba [email protected] The appearance of chronical degenerative deseases has a close relationship with the DNA damage in somatic cells. The damage can be originated by different genotoxic agents, as for example the aromatic Introduction: amines. At the present it’s considered as deseases possible way prevent and treatwith these the use The appearance of chronical degenerative has to a close relationship theaffections DNA damage in of chemoprotective fitocompounds. It has been studied that the total aqueous extract of the cuban specie somatic cells. Phyllanthus K reduce significantly the damage by the aromatic amines: m-PDA), 4The damage Orbicularis can be originated by different genotoxic agents,induced as for example the aromatic amines. aminobiphenyl (4-ABP) y 2-amino1-methyl-6-phenylimidazo[4,5-b]py (PhIP) and offrom the total At the present it’s considered as possible way to prevent and treat theseridine affections the use aqueous fractioned extract was isolated and identified the 2,6 – disecbutilphenol (DSBP). However, it is not chemoprotective fitocompounds. how studied the DSBP at aqueous a molecular level.ofThank’s to the the advances in Orbicularis computational chemistry Itknown has been thatprotects the total extract the cuban specie Phyllanthus K reduce and modelations tools is posible the directamines: chemicalm-PDA), interaction between the DSBP andy the aromatic significantly the damage inducedtobystudy the aromatic 4-aminobiphenyl (4-ABP) 2-aminoamines as possible genoprotective mechanism. The from differents association between the phenol 1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) and the total aqueous complexes fractioned extract was and the and aromatic amines were– disecbutilphenol generated by Multiple Minimum Hypersurface (MMH) and optimized with isolated identified the 2,6 (DSBP). the semiempirical methods AM1 PM6 – d and The association energies complex However, it is not known how the and DSBP protects at PM7. a molecular level. Thank’s to thefor theeach advances in were obtaining with the program Q3. The resultstools allowed demonstrated thatdirect the association between the phenol computational chemistry and modelations is posible to study the chemical interaction between andDSBP the amines was thermodynamically favored genoprotective and it also can mechanism. be predicted a genoprotective order for the the and the aromatic amines as possible phenol against each amine.This research work allow us to propose the chemical direct interaction between Materials and Methods: the phenol andassociation the aminescomplexes as possiblebetween molecular DNA protection. The differents themechanism phenol and for thethe aromatic amines were generated by Multiple Minimum Hypersurface (MMH) and optimized with the semiempirical methods AM1 and PM6 – d and PM7. The association energies for each complex were obtaining with the program Q3. Results: The results allowed demonstrated that the association between the phenol and the amines was thermodynamically and it also canorbicularis be predicted a genoprotective orderphip for the phenol against Key words: dna,favored in sílico, phyllanthus k, dsf, m – pda, 4 - abp, each amine amine. Conclusions 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-058 Malpighia glaba L. (Acerola): analytical studies García Peña, Caridad Margarita; García Borges, Lisandra; Martínez Espinosa, Vivian; Nogueira, Antonio; González, Maria Lidia Centro de Investigación y Desarrollo de Medicamentos (CIDEM), Cuba [email protected] Introduction: Malpighia glaba L. (Acerola, Miguel variety) has beneficial health virtues, is used in traditional medicine as an immunostimulant, detoxifying, remineralizing, among others. studies Introduction: Malpighia glaba L. (Acerola, Miguel variety) has beneficial healthMethods: virtues, isagricultural used in traditional were conducted at the Experimental Station remineralizing, of Medicinal Plants JuanMethods: Tomas Roig of thestudies Drugs medicine as an immunostimulant, detoxifying, amongDr. others. agricultural Research and Development Center Station (CIDEM), determining the Dr. optimal time. of While technological were conducted at the Experimental of Medicinal Plants Juan harvest Tomas Roig the Drugs and analytical studies wereCenter conducted in the researchthe department belonging to thetechnological same center, Research and Development (CIDEM), determining optimal harvest time. While demonstrating throughwere a chromatographic method by High Performance Liquid Chromatography and analytical studies conducted in the research department belonging to the same center, (HPLC), applicable to the qualityacontrol and stability study the high content of Vitamin which is attributed to their demonstrating through chromatographic method by High Performance LiquidC,Chromatography (HPLC), therapeutic Simultaneously studies identification by Thin Layer Chromatography applicable toeffects. the quality control and phytochemical stability study the high for content of Vitamin C, which is attributed to their (CCD) of different in the plant material is performed in addition specifications established therapeutic effects. components Simultaneously phytochemical studies for identification by Thin Layer quality finished product the method of K tabulated. Results: agricultural studiesinidentified Chromatography (CCD)using of different components in the plant material is performed addition the best time of year to harvest the plant material. Theproduct methodusing for controlling and stability studyagricultural was specific, specifications established quality finished the methodquality of K tabulated. Results: accurate, linear, accurate in the concentrations studies quality provedand the studies identified the best time of range year toofharvest the plant studied. material. The The phytochemical method for controlling presence of saponins and flavonoids in plant material. Conclusions: a raw materialstudied. of natural stability study was specific, accurate, linear, accurate in the range of concentrations Theorigin that meets quality specifications required for the development of pharmaceutical products was Conclusions: obtained. phytochemical studies proved the presence of saponins and flavonoids in plant material. a Key words: Malpighia glaba L., Acerola, analytical study 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-059 Effect of dietary supplementation with sanguinarine on biological indicators in growing Martínez Aguilar, Yordan; Liu, G.; Yin, Y. L.; Rodríguez, R.; Valdivié, M.; Más, D. Universidad de Granma, Cuba [email protected] This study was conducted to determine the effect effect of of dietary dietary supplementation supplementation with with sanguinarine sanguinarine on on growth growt h performance, serum serummetabolites metabolites and and antibodies antibodies in growing in growing pigs. A total pigs. of 120 A total [Yorkshire× of 120 [Yorkshire× (Duroc×Landrace)] pigs, pigs, at at 66weeks weeksofofage age(3(3 weeks weeks postweaning) postweaning) were were randomly randomly allotted allotted to 3to dietary 3 dietary treatments. The dietary treatments were: the basal basal diet diet (BD)-fed (BD)-fed group, group, the the sanguinarine sanguinarine (sangrovit®)-fed (sangrovit®)-f ed group (BD+0.04 g/kg), and the antibiotic-fed group group (BD+0.2 (BD+0.2 g/kg g/kg colistin) colistin) (n=40 (n=40 per per group).On group).On days days 7, 7,14 14 and 28 after after initiation initiationofoftreatment, treatment,serum serumlevels levelsofofamino amino acids acids (AA), (AA), antibodies antibodies andand some some biochemical biochemical parameters were were measured. measured. Sanguinarine Sanguinarine used usedasassupplemental supplementalincreased increased(P<0.05) (P<0.05) thethe average average daily daily feed intake (ADFI), (ADFI), average average daily daily gain gain(ADG), (ADG),nutrient nutrientintakes intakesand andfinal finalbody bodyweight weight (BW), (BW), improved improved thethe feed/gain ratio (F/G) (P<0.05), and and reduced reduced (P<0.05) (P<0.05) the the incidence incidence of ofdiarrhea diarrhea iningrowing growing pigs pigscompared compared with the non-additive and antibiotic groups. Results indicated that the sanguinarine increased (P<0.05) the serum contents of AA, such asasGly, Ala and and Thr Thrinincomparison comparisonwith with antibioticthe serum contents of AA, such Gly,Ile, Ile,Lys, Lys,Met, Met, Arg, Ala thethe antibioticsupplemented group and/or BD BD on on days days of of trial. trial. InIncontrast contrasttotocolistin, colistin,sanguinarine-fed sanguinarine-fed group groupdecreased decreased (P<0.05) serum serum level level ofoftriglycerides triglyceridesand andincreased increased (P<0.05) concentration of glucose, as well as this (P<0.05) concentration of glucose, as well as this medicinal extract enhanced (P<0.05) serum concentration of IgG, but only on day 7 in relation to the other 2 groups. These These findings findings indicate indicateaabeneficial beneficialeffect effectofofthe the sanguinarineonon growth performance, serum sanguinarine growth performance, serum concentration of some essential and non-essential AA, humoral immune response and diarrhea incidence, which may offer an effective alternative to antibiotics for growing pigs. Key words: sanguinarine, pig, amino acid, serum antibody, serum metabolite, growth performance 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-060 Development and validation of a high performance liquid chromatographic method to the quantitation of phytosterols in Portulaca oleracea L Alba de Armas, Miguel Ángel; Bravo Sánchez, Luis Ramón; Dumenigo Peña, Lismeivy; Gómez Reyes, Imilsys Universidad Central "Marta Abreu" de Las Villas (UCLV), Cuba [email protected] Purslane (Portulaca oleracea L.) is is aa widely widely extended extended plant plantininCuban Cuban fields fields and anditsitsuse useforforalimentary alimentaryand and medicinal purposes purposes isiswell wellknown. known.Traditionally Traditionally it has employed as diuretic, anti-helmintic, it has beenbeen employed as diuretic, anti-helmintic, anti- antiinflammatory, etc. etc. ItIt isisreported reportedthat that Portulaca oleracea L. can reduce atherosclerosis prolonged Portulaca oleracea L. can reduce atherosclerosis after after prolonged hypercholesterolemia due to the presence of phytosterols which are able to inhibit the intestinal absorption of cholesterol. In this work, method High Performance Liquid Chromatography (HPLC) for(HPLC) qualitative absorption of cholesterol. In athis work, by a method by High Performance Liquid Chromatography for and quantitative analysis ofanalysis stigmasterol and ß-sitosterol in Portulaca oleracea L. powder L. was developed. qualitative and quantitative of stigmasterol and ß-sitosterol in Portulaca oleracea powder was A Lichrospher RP-18 (5 µm) 125 (5X µm) 4 mm column (reversed phase); isocratic developed. A Lichrospher RP-18 125chromatographic X 4 mm chromatographic column (reversed phase); operation isocratic mode and mode UV detection a wave at length of 208 nmof were method was validated operation and UV at detection a wave length 208 set. nm The weredeveloped set. The developed method was for the quality control ofquality the mentioned powders by means of thebyfollowing parameters: linearity, precision, validated for the control ofplant the mentioned plant powders means of the following parameters: accuracy, specificity and detection / quantification as described inasinternational The linearity, precision, accuracy, specificity and detectionlimits, / quantification limits, described instandards. international developed allowed to simultaneously determining stigmasterol andstigmasterol ß -sitosterol in standards. and The validated developedtechnique and validated technique allowed to simultaneously determining Portulaca oleracea L. powders. A 0,243L.% (dry base) of stigmasterol and of 0,815 % (dry base) of ß-sitosterol and ß-sitosterol in Portulaca oleracea powders. A 0,243 % (dry base) stigmasterol and 0,815 % (dry were obtained from quantitative analysis. The HPLC method was reliable and could be employed as a base) of ß-sitosterol were obtained from quantitative analysis. The HPLC method was reliable and could quality control method. Key words: phytosterols, hypercholesterolemia, hplc, validation, portulaca oleracea l 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-061 Toxicity studies of Curcuma longa extracts Quintana Dovales, Neisa ;García Martín, Juan de Jesús Laboratorio de Fito y Apifármacos (Pinar del Río), Cuba [email protected] The CUMIN EXTRACT is a pharmaceutical form prepared from the Curcuma longa L.. The Curcuma longa L. is harvested from the north-east of Pinar delform Rio prepared province, from Cuba.the From 1995 was isolated antirretroviral The CUMIN EXTRACT is a pharmaceutical Curcuma longa L.. TheanCurcuma protein of 18-19D molecular weightdel(Garcia Martin J. et al From 2003.NPU).Toxicity in Curcuma longa L.(Tyrphostines) is harvested from the north-east of Pinar Rio province, Cuba. 1995 was isolated an longa extractsprotein was our principal objective in this case. In weight first place biological antirretroviral (Tyrphostines) of 18-19D molecular (Garcia Martinassays J. et al were realized using 40 female mice frominSwitzerland Stockextracts of-1 (Barcelona), with maintenance Extract of 2003.NPU).Toxicity Curcuma longa was our principal objective in diet this using case. Aqueous In first place Curcuma Twenty mice using received extract mice and twenty were the control This group with received a biological longa assaysL.were realized 40 female from Switzerland Stock group. of-1 (Barcelona), standard diet.diet Experimental studies with 4mg/kg mouse per L. day= 0.4 mg/Kg mouse per day and withtwenty curcumin maintenance using Aqueous Extract of Curcuma longa Twenty mice received extract in human doses.group. The evaluation to the mice was in the Thomas measure, weight were the control This group made received a standard diet.about Experimental studies media with 4mg/kg mouse per sequence, muscular coordination method, String-Test Miguel The Blasco ( Don't fall in atorow), Spontaneous day= 0.4 mg/Kg mouse per day with curcumin in humanof doses. evaluation made the mice was explorer (Labyrinth in T:measure, explore the first sequence, arm of the muscular labyrinth in 60 seconds). Passing four weeks about in the Thomastest media weight coordination method, String-Test of the mice sacrificed andinwe take Spontaneous blood from the neck, (Labyrinth a piece oftest liverin (Lipid peroxidation) looking Miguelwere Blasco ( Don't fall a row), explorer T: explore the first arm of thefor Malonialdehyde concentration by thiobarbituryc and sacrificed proteins concentrated Lowry As aa labyrinth in 60 seconds). Passing four weeks themethod mice were and we take by blood frommethod. the neck, result of liver this research we obtained that Curcuma longa extracts didn't cause toxicity in method mice, besides piece of (Lipid peroxidation) looking for Malonialdehyde concentration by any thiobarbituryc and decreased lipids peroxides in blood and finally the 25ofhigher doses ofweCurcuma not toxics. longa proteins concentrated by Lowry method. As a result this research obtainedare that Curcuma Key words: toxicity studies, Curcuma longa, cumin 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-062 Functional Characterization of an antifungal peptide from marine invertebrate Cenchritis muricatus (Gastropoda: Littorinidae) Lugo Álvarez, María Bárbara; Alba Menéndez, Annia; Garay, Hilda E.; Campos Dias, Simoni; Luiz Franco, Octavio; López Abarrategui, Carlos; Otero González, Anselmo J. Institute of Tropical Fruit Growing, Cuba [email protected] The search of new new molecules molecules from from natural natural sources sources to totreat treat infectious infectious and andparasitic parasiticdiseases diseaseshas haspointed pointed antimicrobial peptides as a promissory alternative. alternative. Besides, Besides, the the important important role role of ofthese thesepeptides peptides inininnate innate immunity, especially especiallyinininvertebrates, invertebrates,makes makes this this groupgroup of animals of animals a secure a source secure for source obtaining for obtaining antimicrobial peptides. Due Due to to the thedifferent different characteristics characteristicsand andversatility versatilityininfunctions functionsof of these these molecules molecules and the continuous development development of of resistance resistance to toconventional conventional antibiotics antibioticsininmany manymicrobial microbialstrains; strains;the theaim of to improve the antimicrobial activity of Cm-p1, a peptide isolated of the mollusk Cenchritis aimthis of work this work to improve the antimicrobial activity of Cm-p1, a peptide isolated of sea the sea mollusk muricatus. It was performed from synthetic peptides from Cm-p1taking into account Cenchritis muricatus. It was performed from derivative synthetic derivative peptides from Cm-p1taking intoproperties account properties such as Boman such as index, Boman cationicity index, and cationicity mass weight and mass of the weight original of the fragment originaloffragment Cm-p1, parameters of Cm-p1, currently parameters related to antimicrobial currently related activity. to In antimicrobial order to accomplish activity. Inour order objective, to accomplish the antibacterial our objective, and antifungal the of the antibacterial three synthetic andpeptides antifungal Cm-p3, of theCm-p4, three synthetic Cm-p5 were peptides evaluated Cm-p3, and Cm-p4, compared Cm-p5 with were Cm-p1. evaluated They showed and compared a little and with null Cm-p1. antibacterial They activity showedinacontrast little andtonull antifungal antibacterial activity activity which in was contrast increased to antifungal mostlyactivity Cm-p5. whichpeptide This was increased resulted, mostly conveniently, Cm-p5. non-toxic This peptide for animal resulted, cells conveniently, in vitro, at relatively non-toxichigh for animal concentrations. cells in vitro, Key words: antibacterial activity, antifungal activity, antifungals, minimum inhibitory concentration 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-063 Applications of essential oil clove as fungicide on environments of archives and libraries Valdés Pérez, Oderlaise; Borrego Alonso, Sofía Flavia; Anaya Villalpanda, Matilde; Vivar González, Isbel; Molina Veloso, Alian; Rodrìguez, Julio C. Archivo Nacional de la República de Cuba, Cuba [email protected] The preservation of of historical historical memory memory has hasbeen beena apriority priorityof of allall civilization. civilization. Archives Archives andand libraries libraries hoard hoard much of the the documentary documentary heritage heritage on onpaper. paper.Though, Though,sometimes sometimesthere thereareare problems problems of biodeterioration of biodeterioration caused by by fungi, fungi,conditioned conditionedmainly mainlyby by fluctuations fluctuations of environmental of environmental parameters parameters whichwhich promoted promoted the the development thereof. For this in the the biodeterioration biodeterioration control, control, chemicals chemicals have have been been used, used,although althoughitithas hasnot been a definitive solution, as as these staff, the thedocument documentand andthe the environment , not been a definitive solution, thesemay mayaffect affect the the health health of staff, environment, therefore is continued in the search for safer products. products. At present, present, scientific scientific studies studies are are conducted conducted in in order to findtoalternatives for controlling biodeterioration by using plant plant products as biocides. The The present study order find alternatives for controlling biodeterioration by using products as biocides. present study to aims aims evaluate to evaluate the antifungal the antifungal activityactivity of the of essential the essential oil of clove oil of clove against against three three strainsstrains from environment from s environments of the repositories of theofrepositories the NationalofArchives the National of the Archives Republic of the of Cuba Republic and its of Cuba effect and on paper. its effect Theonfollowing paper. The followingwere procedures procedures used: the were wells used: used: method the the wells and wellsthe method method tube and dilution and the thetube method, tubedilution dilution biofilm method, method, inhibition biofilm biofilm test inhibition inhibition and biocidal test test and biocidal activity test on activity papertest colonized on paperbycolonized colonized fungi. Onby paper fungi. fungi.was On measured paper paper was was the measured measured copper the the number, copper copper the number, number, pH value the theand pH pH value the appearance and the appearance of stains before of stains andbefore after accelerated and after accelerated again. The again. resultsThe showed results biocidal showed activity biocidal to values of 3% oftothe oil, affectation in the theconidiogenesis, formation of biofilms was inhibited as well as inhibited the fungal activity values of 3% of the oil,conidiogenesis, affectation in the the formation of biofilms was colonization. Also, there was no significant changes in the paper pH, no structural changes in the paper, or stains on them. Key words: essential oils, vegetable biocide, fungicidal activity, biodeterioration, documentary heritage 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-064 Hypocholesterolemic and antiatherosclerotic effects of crude leaf extract of Moringa oleifera in Apoe Ko Mice Fernández, Nidia; Fuentes, D.; Díaz, B. L.; León, A.; Blanco, D.; Gutiérrez, M.; García, A.; Ronda, M.; Prida, Y.; Torres, Y.; Cabezas, L. Centro Nacional para la Producción de Animales de Laboratorio (CENPALAB), Cuba [email protected] elevated concentration of total in plasma with Numerous population population studies studieshave havelinked linked elevated concentration of cholesterol total cholesterol in plasma with oleifera Lam (Moringaceae) (Moringaceae) are are used increased incidence of atherosclerotic events. The leaves of Moringa oleifera used in many in many cultures cultures as as a hypocholesterolemic a hypocholesterolemicagent agent inin obese patients. patients.Taking Takinginto into consideration consideration thatthat B6.129P2-Apoetm1Unc/J mice miceexhibit exhibita marked a marked increase increase in plasma in plasma cholesterol cholesterol level and level develop and develop atherosclerotic lesions resembling those those seen seen in in humans; humans; the the main mainobjective objective ofofour ourstudy studywas wastotoevaluate evaluat e the hypocholesterolemic and anti-atherosclerotic effects of crude extract extract of of leaf leaf of of Moringa Moringa oleifera oleifera in in ApoE ApoE KO mice. KO Males mice. Males and females and females B6.129P2-Apoetm1Unc/J B6.129P2-Apoetm1Unc/J KO with KO4 with - 8 weeks 4 - 8 weeks of age of were agefed were ad fed libitum ad with libitum diet. Theanimals half of the fed leaf with extract crude leaf extract of Moringa oleifera during normal with diet.normal The half of the wasanimals fed withwas crude of Moringa oleifera during 90 days and 90 days the other control. C57Bl/6 miceused wereas used as control. weight, the otherand served as served control.asC57Bl/6 mice were control. Body Body weight, foodfood consumption, consumption, haematological andparameters, biochemicalrelative parameters, weightand of the organs and aortic lesions haematological and biochemical weight relative of the organs aortic atherosclerotic atherosclerotic It was found that administration crude oleifera leaf extract of were measured.lesions It was were foundmeasured. that administration of the crude leaf extractofofthe Moringa decreased the Moringa oleifera decreased in comparison with control cholesterol increases in ApoE theKO cholesterol mice serum increases in comparison in ApoE KO withmice control serum group, while no changes were group, inwhile no changes plaque formation in aorta found wild type mice (C57Bl/6). were found Atherosclerotic in wild type mice plaque (C57Bl/6). formationAtherosclerotic in aorta was also reduced approximately was alsoinreduced 35 % inIt treated ApoE KO mice. wasleaves concluded that the leaves of have 35 % treated approximately ApoE KO mice. was concluded that Itthe of Moringa oleifera Moringa oleifera have and hypocholesterolemic hypocholesterolemic anti-atherosclerotic andactivity anti-atherosclerotic in B6.129P2-Apoetm1Unc/J activity in B6.129P2-Apoetm1Unc/J mice and that there is valid pharmacological basis for employing them for this purpose in humans. Key words: atherosclerosis, apoe mice, hypocholesterolemic, moringa oleifera 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-065 Effect of Korean native chicken meat on pro-inflammatory cytokine gene expression Hye Kyung, Kim; Myung Gyou, Kim; Kang Hyun, Leem Hanseo University, Turkey [email protected] Introduction: Chicken essence is regarded as as aa health health food food in in Chinese Chinese communities communities and and South South East EastAsia. Asia. In western countries, chicken soup soup has has also also been been traditionally traditionally used usedfor forrecovery recovery from fromcold. cold.Present Presentstudy study investigated the anti-inflammatory effects effects of of four four different different Korean Korean native native chickens chickens(YeonsanOgye, (YeonsanOgye, Hyunin Hyunin Black, Hwangbong, Hwangbong, and andHoengseongYakdak). HoengseongYakdak).White White Leghorn Leghorn waswas usedused as a as control. a control. Materials and Materials and Methods: Breast Methods: meatsBreast were meats grounded were and grounded extracted and extracted with 70%with ethanol 70% ethanol at 60 oC at 60 foroC6 for hours.The 6 hours.The concentrationof concentrationof released histamine released was histamine determined was determined in compound in compound 48/80-induced 48/80-induced HMC-1 cellHMC-1 supernatant cell s supernatants by spectrofluorometric by spectrofluorometric procedure. Quantitative procedure. real-time Quantitative polymerase real-time chain polymerase reactionchain (RT-reaction PCR) was (RT-used PCR) to quantify was used the expression to quantifyofthe mRNAs expression for IL-4, of mRNAs IL-10, IFNfor IL-4, , andIL-10, TNF-?IFNin LPS-stimulated , and TNF-? in RAW LPS-stimulated 264.7 cells. RAW 264.7 Results: Histamine cells. release was not affected by all strains of breast or leg meat extracts. However, breast Results: meat extracts Histamine of fourrelease strainswas of Korean not affected nativebychicken all strains significantly of breast or reduced leg meat theextracts. gene expressions However, breast of IL-4 meatIFNand extracts while of White four strains Leghorn of Korean fail to affect nativethese chicken parameters. significantly IL-10 reduced mRNAthe expressions gene expressions were decreased of IL-4 andHwangbong IFN- while White Leghorn fail to affect breast these parameters. IL-10 mRNA were decreased by and HoengseongYakdak meat extracts, and TNF- expressions gene expressions were not by Hwangbong changed by chicken and HoengseongYakdak meat extracts. Conclusion: breast Present meat extracts, study suggests and TNF-that gene breast expressions meat of Korean were not native changedmanifest chicken by chicken its meat anti- inflammatory extracts. effect, at least in part, by regulating the pro-inflammatory cytokine Conclusion: gene expressions. Present study suggests that breast meat of Korean native chicken manifest its anti- Key words: korean native chicken, raw 264.7 cells, pro-inflammatory cytokine, gene expression, il-4, il-10 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-066 Evaluation of the use of fibre dietary in ice cream M Boumba, Aniely; Castro, Yanires; Rodríguez, Tamara Instituto de Investigación para la Industria Alimenticia, Cuba [email protected] It was evaluated the use of microcrystalline cellulose in the milk ice cream formulation by means of the global evaluation of the product and its sensorial quality. For this purpose there were rehearsed different proportions of the dietary fibre and in parallel was elaborated the control ice cream of milk. It was evaluat ed the viscosity of the mixtures and the melting, yield and sensorial quality of the ice creams. To the selected variant was evaluated the composition and general characteristics. With the level of 4.5 % of microcrystalline cellulose was achieved a product of very good sensorial quality and satisfactory melting and nutritional characteristics. Key words: microcrystalline cellulose, ice cream 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-067 Development of fermented milks from buffalo´s semiskimmilk and culture of lactobacillus acidophilus M Boumba, Aniely; Iñiguez, Carola; Castro, Yanires Instituto de Investigación para la Industria Alimenticia, Cuba [email protected] Pitching rate influence of industrial culture of Lactobacillus acidophilus on incubation time, acidity and pH of fermented milk from buffalo ´s semi skim milk and 8 % of sugar, were studied. The best formula, with inoculation rate and emulsion, was used for producing batches of fermented milk at pilot scale to conduct physicochemical and microbiological analysis, and a sensory acceptance test. Shelf life was determined trough the graphic method for incomplete failurer data using Weibull´s distribution. It was concluded that the product showed good phisico - chemical, sensory and microbiologic characteristics. Shelf life at 4ºC was 15 days during witch it maintains a cellular viabiity of was 10 8 UFC/g. In the mass acceptance test this fermented milk rated within the "I like very much" and "I like extremaly intervals". Key words: fermented milk, buffalo's semi skim milk, lactobacillus acidophilus 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-068 Soy ice cream for diabetics Castro, Yanires; M Boumba, Aniely; Rodríguez, Tamara Instituto de Investigación para la Industria Alimenticia, Cuba [email protected] The present work had as objective to obtain a frozen and and functional dairy dairy dessert, of of appropriate appropriate nutritional nutritional value, that value, benefits that thebenefits consumers the consumers and especially and to especially the populations to the populations clinically needy clinically as the needy diabetics. as theIn this diabetics. ice cream is present the soy because of its low glucémic index, their composition with a high level of dietary In thislow-level fiber, ice cream ofisfatpresent and carbohydrates the soy because where of its thelow sugar glucémic is not present. index, their There composition were carefully with a selected high levelthe of dietary raw materials fiber, to low-level use, keeping of fat and in carbohydrates mind the intention whereathe developing sugar is not a low present. Calorie There icewere cream. carefully Different selected the raw formulations werematerials evaluatedto where use, keeping sorbitolinwas mind present, the intention in order a developing to increase athe lowtotal Calorie solids, iceviscosity cream. and Different formulations sweetness, together fruit were pulps evaluated that imparted where freshness sorbitol was andpresent, good flavour. in orderIt to is concluded increase the that total is itsolids, possible viscosity the obtaining and an sweetness, ice creamtogether of satisfactory fruit pulps sensorial that imparted and nutritional freshness characteristic, and good flavour. with lowItcontain is concluded of fat and that a caloric is it possible value 35% thelower obtaining than an theice traditional cream ofice satisfactory cream, for sensorial feeding ofand diabetics nutritional and characteristic, other needful clinically with low contain sectors. In the of fat massive and a caloric acceptance value 35% evaluation lower than of the theproduct, traditional 80% iceofcream, the consumers for feedingevaluated of diabetics theand ice other needful sectors. In the massive acceptance evaluation of the product, 80% of the cream as I likeclinically it very much. consumers evaluated the ice cream as I like it very much. Key words: diabetics, soy milk, sorbitol, hipocaloric sweeters, ice cream 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-069 Use of cheese whey in ice cream Castro, Yanires; M Boumba, Aniely; Rodríguez, Tamara; Seivanes, Ariel Instituto de Investigación para la Industria Alimenticia, Cuba [email protected] It was evaluated the use of whey from cheddar cheese as substitute of non fatty solids of milk in ice cream formulation, by means of the global evaluation of the product and its sensorial quality. Different substitution levels (15, 25 and 35%) and ice cream control were elaborated at laboratory scale. The viscosity of de mixtures, melting, yield and sensorial quality of the ice cream were evaluated. To the selected variant, the composition and general characteristics were determined at pilot plant scale. With the lever 25% of substitution of non fatty solids of milk for solids of whey, a product of very good sensorial quality, excellent melting, yield, and satisfactory nutritional characteristics was achieved. Key words: cheese whey, ice cream 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-070 Potentialities of leaves from Clusia minor L. species Boffill Hernández, Guillermo; Mangas Marín, Raisa; Reynaldo Fernández, Gledys; Robeiro Guerra, Idania; Bello Alarcón, Adonis Centro Nacional de Investigaciones Científicas (CNIC), Cuba [email protected] Los estudios fitoquímicos realizados al género Clusia (Guttiferae) han demostrado que es una fuente rica de secundarios. En estealestudio propusimos determinar la capacidad secuestradora del Losmetabolitos estudios fitoquímicos realizados géneronos Clusia (Guttiferae) han demostrado que es una fuente rica de metabolitos DPPH y antiinflamatoria secundarios. de En cuatro este extractos estudio nos obtenidos propusimos a partir determinar de las lahojas capacidad de la secuestradora especie endémica del ClusiaminorL. Los extractos se prepararon maceración en orden polaridadendémica (hexano (CMHDPPH y antiinflamatoria de cuatro extractospor obtenidos a partir de lascreciente hojas de de la especie A), acetato de etilo (CMH-B) ysemetanol (CMH-C)) y por extracción directa con etanol (CMH-E)).Los estudios ClusiaminorL. Los extractos prepararon por maceración en orden creciente de polaridad (hexano por cromatografía enetilo capa(CMH-B) delgaday revelaron las diferencias cualitativasdirecta de estos extractos.El tamizaje (CMH-A), acetato de metanol (CMH-C)) y por extracción con etanol (CMH-E)).Los fitoquímicocorroboró estas en diferencias cualitativas se diferencias detectaron cualitativas principalmente compuestos como: estudios por cromatografía capa delgada revelarony las de estos extractos.El flavonoides, triterpenos, compuestos fenólicos.La actividad ysecuestradora radical, mediante el ensayo tamizaje fitoquímicocorroboró estas diferencias cualitativas se detectarondel principalmente compuestos de DPPH, mostró que las fracciones CMH-Bfenólicos.La y CMH-E presentaron un efecto significativo, mientras queella como: flavonoides, triterpenos, compuestos actividad secuestradora del radical, mediante actividad en CMH-B el modelo de edema en la oreja ratónsignificativo, inducido por aceite ensayo deantiinflamatoria DPPH, mostró de queloslasextractos fracciones y CMH-E presentaron un de efecto de crotónque mostraron un alto por ciento de de actividad en todos extractos excepción del extracto mientras la actividad antiinflamatoria los extractos en los el modelo decon edema en la oreja de ratónpolar CMH-C. inducido por aceite de crotón mostraron un alto por ciento de actividad en todos los extractos con Key words: género clusia, actividad antioxidante, actividad antiinflamatoria, tamizaje fitoquímico, 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-072 Almonds consumption to reduce cholesterol and increase human health Rodríguez Diego, Jesús G.; Villoch, Alejandra; Melo Ruíz, Virginia; Sánchez Herrera, Karina Universidad Autónoma Metropolitana-Xochimilco, México [email protected]; [email protected] Almonds Intake as a tidbit snack, or or as as an an ingredient ingredient of of numerous numerous kind kind of ofgourmet gourmet dishes dishes either either ininstews stewsor or desserts in in desserts is aiscommon a common practice practice by by population population all all over over thethe world, world, however, however, nutritional nutritional value value is not is not take take into into account. account. Almonds Almonds are high are high in macronutrients in macronutrients mainly mainly in fatty in fatty acids, acids, not not onlyonly in monounsaturated in monounsaturated MUFA MUFA but but also also in polyunsaturated in polyunsaturated PUFA PUFA fats,fats, dietary dietary fiber,fiber, and and a variety a variety of phytonutrients of phytonutrients that that believed believed to have to have cardio cardio protective protective attributes. attributes. This study This was studyconducted was conducted to determine to determine the effects the effects of almonds of almonds intakeintake on blood on lipids bloodtotal and lipidscholesterol. and total cholesterol. Observation Observation of 120 person's of 120 person's age of 50 agetoof65, 50 to males 65, males and females and females with with normal normal cholesterol cholesterol and hypercholesterolemia and hypercholesterolemia with consumption with consumption of 25 toof 125 25 g/day to 125per g/day individual per individual during 18 during weeks, with their own diet. Cholesterol were assess before andbefore after the Total cholesterol 18 weeks, withregular their own regular diet. Cholesterol were assess andstudy. after the study. Total presents a little reduction, 4.5% andreduction, 5.5% respectively. The result of this The study show a cardio effect cholesterol presents a little 4.5% and 5.5% respectively. result of this study protective show a cardio associated with nut consumption. Cholesterol levelCholesterol is affected by age, sex and by diet, andsex plays significant protective effect associated with nut consumption. level is affected age, andadiet, and playsina different role significantmetabolism role in different functions metabolism in human functions health.in Almonds human health. consumption Almonds lowers consumption total cholesterol; lowers total cholesterol; however more studies however regarding more studies this matter regarding should this be matter performance, should be taking performance, in consideration taking in fatty acids consideration content of almonds fatty acids and diet content and of increase almonds theand participant's diet and increase number the in sample participant's for further number studies. in sample Almonds for should be incorporated as a regular habit in a daily diet, mainly in adults with cholesterol problems. Key words: almonds consumption; to reduce cholesterol; human health 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-073 Enzymatic hydrolysis of whole bovine plasma protein: optimization and enzyme activity of peptide fractions obtained by ultrafiltration Figueroa Moreno, Omar Alfredo ; Zapata Montoya, José Edgar Universidad Popular del César, Colombia [email protected] In this study, the thereaction reaction of ofenzymatichydrolysis enzymatic hydrolysisofofproteinsfromwholebovine proteins from whole bovine plasma plasma wasoptimized was optimized in ain a batch reactor, using usingthemethodology the methodology of of response response surface (MSR), the theantioxidantactivity antioxidant activityof of peptide fractions peptidefractionswereanalyzedusinganalyticalmethodssuch were analyzed using analytical methods such as ABTS and as FRAP ABTS and and system FRAPultrafiltration and fractionation of systemultrafiltrationfractionation antioxidant peptides interest was of antioxidantpeptidesinterestwasdesigned. designed. Alcalase 2.4L was used for Alcalase the hydrolysis, 2.4L where three experimental factors: pH,wherethree temperatureexperimental and enzyme-substrate (E /S) ratio,and were analyzed in order wasusedforthehydrolysis, factors: pH, temperature enzyme-substrate (E / to maximize the degree ofinhydrolysis (DH) reaction fort wohours reaction. GH was measured using the S) ratio, wereanalyzed order to maximizethedegree of hydrolysis (DH) The reactionfortwohoursreaction. method of valuation of theproton (pH-stat). The effect of GH on(pH-stat). experimental factors wasonadjusted to a The GH wasmeasuredusingthemet hod of valuation of theproton Theeffect of GH quadratic equation which has coefficient of determination of has 0.931. Optimalofreaction conditions for the experimental factorswasadjusted to a quadraticequationwhich coefficient determination of 0.931. enzymatic hydrolysis of bovine plasma were: Temperature ° C, were: pH 9 Temperature and E / S 10% / w). Optimalreactionconditionsfortheenzymatichydrolysis of bovine60.4 plasma 60.4(w° C, pHThe 9 predicted for/ GH 23%. Ananalysis of experimental factors evidences the dual effect and E / S value 10% (w w). was Thepredictedvaluefor GHinteraction was 23%.ofAnanalysis of interaction of experimental factorsevidencesthe of temperature and pH, dualon effect the other of temperature hand the and direct pH, relationship ontheotherhandthedirectrelationship of the E / S in the assessed of the levels E/S was in theassessedlevelswasnoted. noted. The antioxidant activity of Theantioxidantactivity bovine plasma enzymatic of bovine hydrolysates plasma with Alcalase 2.4 L, increases enzymatichydrolysateswithAlcalase significantly with the GH. Through2.4anL, increasessignificantlywiththe ultrafiltration system is possible GH. is possible to concentrate peptidefractions of molecular <8kDa weight, presenting significant antioxidant activityr elative to the native protein and the complete hydrolyzate. Key words: ultrafiltration Enzymatic hydrolysis, bovine plasma, protein, optimization, enzyme activity, peptide, 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-074 Probiotic fermented milk with prebiotic syrup Rodríguez Martínez, Oxalis; Rodríguez Martínez, José Alberto; Trujillo, Luis E.; Pérez Cruz, Enrique R.; Santos Carballal, Beatriz; Núñez de Villavicencio, Margarita IIIA, Cuba [email protected] Among dairy products products with with functional functional properties, properties, the thesymbiotic symbioticfermented fermentedmilks milksare arecommercially commerciallyavaible avaible world wide. Numerous studies refer that the the incorporation of probiotic probiotic bacteria bacteria and and prebiotic prebiotic product product in in the the for for health. fermented milk milkare arebeneficial beneficial health. Syrup prebiotic was used at 0.3 % and Bioyogur starter Syrup prebioticacidophilus was used at+Streptococcus 0.3 % and Bioyogur starter (Lactobacillus acidophilus +Streptococcus (Lactobacillus thermophilus) was inoculated at 2 % v/v. Lactic acid formation thermophilus) at for 2 %samples v/v. Lactic acid formation and phinoculated values were determined and ph values was wereinoculated determined collected at the recently milk and every for 0.5 h to the samples collected at thecontents recently at inoculated milkcellular and every 0.5 hoftoL.the coagulation. The sugardetermined. contents coagulation. The sugar 0 y 3 h and viability acidophilus at 24 h were at 0 yglobal 3 h and cellular of L.The acidophilus 24 h were determined.plastics The global qualityand wasstored at 4 The quality wasviability evaluated. shelf life at parked in polyethylene containers evaluated. 6 oC was The determined. shelf life The parked acidification in polyethylene capacity plastics and containers growth of and starters stored were at 4not - 6 affected oC was of the used determined. prebiotic syrup. The syrup for growth meanwhile the fructooligosaccharides fraction was intact. The The acidification products had good capacity sensorial, and physics-chemestry growth of starters were and not microbiological affected of the quality. usedThe prebiotic viability syrup. of L. The acidophilus syrup for growthcounts reached meanwhile in order theoffructooligosaccharides 1010 ufc/mL. The shelffraction life waswas 21 days. intact. The products had good sensorial, physics-chemestry and microbiological quality. The viability of L. acidophilus reached counts in order of Key words: probiotic, prebiotic, fermented milk, fructooligosaccharides, symbiotic 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-075 Mangiferin solubility in different solvents used in the pharmaceutical industry Sevilla Fernández, Iliana Universidad de La Habana (UH), Cuba [email protected] Mangiferin an important bioactive constituent of mango with numerous pharmacological properties. The Mangiferin an important bioactive in constituent mango with numerous pharmacological properties. The solubility behavior is important order to of obtain complete information about physicochemical data. solubility behavior is important in order to obtain complete information about physicochemical Otherwise, temperature-solubility dependence allows perform the respective thermodynamicdata. analysis. In Otherwise, dependence allows perform the respective In the present temperature-solubility work, we have measurement the solubility of mangiferin in waterthermodynamic and six organic analysis. compounds the present used work,inwe measurementindustry the solubility mangiferin and six organic Materials compounds commonly thehave pharmaceutical in the of range of 5 toin60water 0C of temperature. and commonly usedmass in theofpharmaceutical of 5 in to the 60 0C of temperature. Materials andin a methods: The mangiferin was industry chosen in tothe be range in excess solvents. This solution was put methods: The mass of mangiferin was chosen to be in excess in the solvents. This solution was put in a glass vessel inside an incubator under constant agitation for about 3 h at work temperature. The solution glass vessel inside an incubator under constant agitation for about 3 h at work temperature. The solution was centrifuged and the samples were filtered through a 0.45 µm membrane. Concentrations were was centrifuged and the samples were in filtered a 0.45 µm membrane. Concentrations were determined by measuring absorbance a UV through spectrophotometer at 254 nm. Results and discussion: The determined measuringincreased absorbance a UV spectrophotometer at 254 nm. and discussion: solubility of by mangiferin withinan increased in the temperature in Results each solvent. MangiferinThe is solubility mangiferin increased with an increased in theand temperature each solvent. Mangiferin is ether, sparingly of soluble in ethanol, slightly soluble in methanol water and in practically insoluble in diethyl sparingly soluble in ethanol, slightly soluble in methanol and water andVan? practically diethyl ether, acetone and n hexane. The solubility were correlated according to the t Hoff insoluble equation,inthe standards acetone and n hexane. The solubility were correlated according to the Van? t Hoff equation, the heat of solution was obtained from the slope of ln Cs vs 1/T plot. Conclusions: Solubility of mangiferin was standards heat of solution was obtained the slope of ln Cs vs 1/T Conclusions: Solubility of determined experimentally in six solventsfrom at different temperatures. Forplot. all cases was observed increased mangiferin was determined experimentally in six solvents at differentThe temperatures. Forsolubility all casesdata was were in the solubility of mangiferin respect to increase of temperature. experimental observed the equation solubility and of mangiferin respect to increase of for temperature. The experimental correlated increased with Van´tinHoff the standards heats of solution the solubility data were correlated with Van´t Hoff equation and the standards heats of solution for the different solvents were calculated. Key words: mangiferin solubility, solvents, pharmaceutical industry 16th International Scientific Congress CNIC'2015 National Center for Scientific Research Address: Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba. www: http://www.cnic.edu.cu/ 50 th Anniversary, 1965 ‐ 2015 SPN-P-076 Assessment of genotoxic and cytotoxic effectsof Phyllanthus plants aqueous extracts in Caulobacter crescentus Menéndez Perdomo, Ivette María ; Sánchez Lamar, Ángel Universidad de La Habana (UH), Cuba [email protected] Plants of the genus Phyllanthus have have been been widely widely used used by by traditional traditional medicineall medicineall over over the theworld. world.InInCuba, Cuba, there is aa plentiful plentiful variety variety and andendemism endemismofofthem, them,and andvarious variousspecies species have haveproven provenantiviral, antiviral, antioxidant antioxidant and antimutagenic properties against against chemical chemicaland and physical physicalmutagens. mutagens.InInorder ordertotoextend extendthe thesearch searchforfor natural compounds compounds presentinggenoprot presentinggenoprotective ective properties propertiesto to other other species species of this of genus this genus growngrown in our in our country, previous previous studies studies about abouttheir theirpossible possiblegenotoxic genotoxicand and cytotoxic cytotoxic properties properties must must be done. be done. In the In the present work, work, the theaqueous aqueousextracts extracts of four of four Phyllanthus Phyllanthus species species were were assessed: assessed: P. williamioides, P. williamioides, P. P. chamaecristoides,P. chamaecristoides, P.microdictyus, microdictyus,and andP.P.epiphyllanthus, epiphyllanthus,atatconcentrations concentrationsranging rangingfrom from 0.10.1 to 2 tomg/ml 2 mg/ml and using using Caulobactercrescentus Caulobactercrescentus cells cellsasasthethe in vitroexperimental in vitroexperimental model.Genotoxicity model. Genotoxicity was assessed was assessed through evaluating evaluating primary primaryDNA DNAdamage damage by by means means of the of SOS the SOS Colorimetric Colorimetric assay.assay. Cytotoxicity Cytotoxicity was was measured throughcolony throughcolony formation formation assay. assay.AsAs a result, a result, the the genotoxic genotoxic and cytotoxic and cytotoxic effectseffects were only were only statistically significant by by way way of of aaDunnett DunnettTest Test(p<0.05), (p<0.05),for forthe thehighest highestconcentration concentrationtested testedand andonly onlyin in In In all all cases the the LD50values werewere higher than than the maximum case of of the theP. P.williamioides williamioidesplant plantextract. extract. cases LD50values higher the maximum concentration assayed and and P. P.chamaecristoidesandP. chamaecristoidesandP. microdictyus microdictyusextracts extractsshowed showeda asurvival survivalrate rateover over are in in correspondence correspondence with withpreceding preceding studies studiesabout aboutthe thegenotoxicity genotoxicityand andcytotoxicity 90%. These outcomes are cytotoxicity of several Phyllanthus species, withexperimental different experimental vitro andand in vivo, of several Phyllanthus species, with different models, inmodels, vitro andin in vivo, they and validat e future researches the possible properties of these plants extracts. they validate futureabout researches about antimutagenic the possible antimutagenic properties of aqueous these plants aqueous Key words: phyllanthus, caulobactercrescentus,genotoxicity, cytotoxicity, plant aqueous extract