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16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-L-001 The science of chemical complex natural products: network pharmacology (Master
Lecture)
Spelman, Kevin
Health, Education & Research in Botanical Medicine, Ashland, United States
[email protected]
drugable
The
The current model
model in
in drug
drug discovery
discovery isisbased
basedon
onthe
theligand-receptor
ligand-receptorparadigm
paradigmasasthe
the
drugableunit.
unit.
The
common analogy of drug action is that of a lock and key, with a drug acting as a selective 'key' that fits into
the 'lock' of aa specific
specific drug
drug target.
target. And
Andwhile
whilethis
thisselectivity
selectivityparadigm
paradigmhas
hasyielded
yieldedefficacious
efficaciousdrugs,
drugs,many
many
drugs act
actvia
viamodulation
modulationof of
multiple
multiple
proteins:
proteins:
Many
Many
effective
effective
drugsdrugs
in oncology,
in oncology,
psychiatry
psychiatry
and antiand antimicrobials act on multiple, rather
rather than single
single targets.
targets. Systems
Systems biology
biology has
has revealed
revealed physiological
physiological function
function
as a robust network structure that strongly suggests
suggests that
that exquisitely
exquisitely selective
selective compounds,
compounds, compared
compared with
with
multitargetdrugs, may
may exhibit
exhibitlower
lowerthan
than
desired
desired
clinical
clinical
efficacy
efficacy
and induce
and induce
more adverse
more adverse
events. events .
Chemically complex botanically-based medicines inherently act on multiple sites and therefore have never
never fully
been
beenunderstood
fully understood
in a reductionist
in a reductionist
ligand-receptor
ligand-receptor
modelmodel
well. However,
well. However,
botanical
botanical
medicines
medicines
fit intofita
into a network
network
pharmacology
pharmacology
paradigm
paradigm
seamlessly.
seamlessly.
Viewing
Viewing
mixtures
mixtures
of natural
of natural
products
products
through
through
thethe
lens
lens
of
network
how we
wecan
can
improvedrug
drug
discovery
utilize
botanical
of networkbiology
biologymay
mayprovide
provide insights
insights into how
improve
discovery
and and
utilize
botanical
medicines for complex diseases.
Key words: science, chemical complex, natural products, pharmacology
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-L-002 Synthesis and evaluation of exo- methylene butyrolactone natural products- can they
be made into drugs (Master Lecture)
Reiser, Oliver
Institut für Organische Chemie, Universität Regensburg, Germany
oliver.reiser@chemie. uni-regensburg.de
Exo-methylene-ƒÁ-butyrolactone natural products are abundant in nature that display broad
biological profiles, among them high
Exo-methylene-ƒÁ-butyrolactone
natural
tumorproducts
activity. Despite
are abundant
their generally
in nature
high
that display broad biological
cytotoxicity,
profiles,
among
these
them
compounds
high tumor
are activity.
considered
Despite
to betheir
unsuitable
generally
for drug
high development,
cytotoxicity, these compounds are
owing their high
considered
to bebut
unsuitable
unspecific
forreactivity
drug development,
with biological
owing
nucleophiles.
their high but unspecific reactivity with biological
We started a program
nucleophiles.
We started
that
a program
combinesthat
thecombines
synthesisthe
of exo-methylene-ƒÁ-butyrolactone
synthesis of exo-methylene-ƒÁ-butyrolactone natural
products
focusingfocusing
on asymmetric
catalysis,[1]
synthesis
of peptide
foldamers
containing ƒÀ-amino acids[2]
natural products
on asymmetric
catalysis,[1]
synthesis
of peptide
foldamers
as
carriers ƒÀ-amino
and evaluation
of as
hybrids
derived
thereof to of
selectively
recognize
specific
containing
acids[2]
carriers
and evaluation
hybrids derived
thereof
to cancer cells. Thus,
following
approachspecific
we arecancer
confident
thatThus,
a large
body ofour
biologically
potent, but greatly neglected
selectivelyour
recognize
cells.
following
approachhighly
we are
natural
products
and analogs
might become
promising
for drug
development
in medicinal chemistry.
confident
that a large
body of biologically
highly
potent, but
greatly
neglected natural
products and analogs might become promising for drug development in medicinal
chemistry.
[1] a) A. Bergmann, O. Reiser, Chem. Eur. J. 2014, 20, 7613; b) A. Kreuzer, S. Kerres,
T. Ertl,
Key
words:
H. Rucker,
synthesis,
S. Amslinger,
evaluation,
O. Reiser,
exo- methylene
Org. Lett.butyrolactone,
2013, 15, 3420.
natural product, drug
[2] a) C. Cabrele, T. A. Martinek, O. Reiser, L. Berlicki , J. Med. Chem. 2014, 57, 9718.
b) .. Berlicki, M. Kaske, R. Gutierrez-Abad, G. Bernhardt, O. Illa, R. M. Ortuno, C.
Cabrele , A. Buschauer, O. Reiser, J. Med. Chem. 2013, 56, 8422; c) L. Berlicki, L. Pilsl,
E. Weber, I. M. Mandity, C. Cabrele, T. A. Martinke, F. Fulop, O. Reiser, Angew. Chem.
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-L-004 Medicinal plants of the brazilian Caatinga biome: from popular use to scientific
studies (Master Lecture)
Guedes da Silva Almeida, Jackson Roberto
Universidade Federal do Vale do São Francisco, Brazil
jackson.guedes@univas f.edu. br
The Caatinga
Caatinga biome
biome(semi-arid
(semi-arid
vegetation)
vegetation)
is a is
highly
a highly
threatened
threatened
biome covering
biome covering
a vast area
a vast
in
area in
Northeastern Brazil and is the source of few studied natural resources. Many medicinal plant species from
from Caatinga
Caatinga
are widely
are widely
knownknown
and used
and in
used
folk in
medicine
folk medicine
and forand
commercial
for commercial
manufacturing
manufacturing
of phytotherapeut
of
ic
phytotherapeutic
products.
Few ethnobotanical
products. Few
and
ethnobotanical
pharmacological
and studies
pharmacological
have beenstudies
undertaken
have in
been
thisundertaken
region, in spite
in of
this great
the
region,
cultural
in spite
and
of biological
the great cultural
diversityand
to be
biological
found there.
diversity
Thetopurpose
be found
of there.
this lecture
The purpose
is to present
of thisresults
lecture
of
research
is to present
carried results
out at of
theresearch
Federal carried
University
out atofthe
Vale
Federal
do São
University
Francisco
of Vale
withdothe
São
species
Francisco
Annona
with the species
vepretorum,
atemoia
Annona
(A. vepretorum,
cherimola Mill.
atemoia
x A. (A.
squamosa
cherimola
L.),
Mill.
Selaginella
x A. squamosa
convoluta,
L.), Selaginella
Hymenaeamartiana,
convoluta, Hymenaeamartiana,
Bromelialaciniosa,
Encholiriumspectabile
Bromelialaciniosa,
and Neoglazioviavariegata,
Encholiriumspectabile
typical
and Neoglazioviavariegata,
species from the Caatinga
typical species
biome.
The fundamentals
from the Caatinga
of thebiome.
main The
methods
fundamentals
for assessing
of the main
the antinociceptive,
methods for assessing
anti-inflammatory
the
,
antinociceptive, anti-inflammatory,
antiulcerogenic
and central nervous
antiulcerogenic
system activity
and central
of a natural
nervoussubstance
system activity
or plant
of aextracts
natural will be
presented.The
mainextracts
chemical
isolatedmain
fromchemical
extracts of
these species
will be
presented
substance or plant
willconstituents
be presented.The
constituents
isolated
from
extracts as
of well
as
thespecies
chemical
of some
oils. Regarding
the pharmacological
activity,
results willthe
be
these
willcomposition
be presented
as wellessential
as the chemical
composition
of some essential
oils. Regarding
presented
on theactivity,
antinociceptive,
antiulcerogenic
andanti-inflammatory,
on the central nervous system.
pharmacological
results willanti-inflammatory,
be presented on the
antinociceptive,
Furthermore,
in vitrophotoprotective
and system.
antioxidant
activity wasthe
also
exact
antiulcerogenictheand
on the central nervous
Furthermore,
in investigated.The
vitrophotoprotective
andmechanism
involved
in the
antinociceptive
and anti- inflammatory
activities isinvolved
not completely
understood but,
at antileast in
antioxidant
activity
was also investigated.The
exact mechanism
in the antinociceptive
and
inflammatory
part
there is the
activities
participation
is not of
completely
opioid receptors
understood
and but,
inhibition
at least
of cyclooxygenase
in part there is the
enzyme.Pharmacologic
participation of opioid al
receptors
and studies
inhibition
cyclooxygenase
and chemical
studies
continuing
and
chemical
areofcontinuing
in orderenzyme.Pharmacological
to characterize the mechanism
responsible
forare
these
effects.
Key words: medicinal plants, phytochemistry, pharmacology, natural products, caatinga
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-O-001 Therapeutic effects of policosanol and atorvastatin against global brain ischemiareperfusion injury in gerbils
Molina Cuevas, Vivian; Ravelo Calzado, Yazmin; Noa Puig, Miriam; Mas Ferreiro, Rosa María; Pérez
Guerra, Yohani; Oyarzábal Year, Ambar; Mendoza Hernández, Nilda; Jiménez Despaigne, Sonia;
Sánchez López, Javier Vicente
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
El ictus isquémico constituye la tercera causa de muerte y la primera de discapacidad en la población adulta.
El uso de agentes antiplaquetarios en el tratamiento del ictus isquémico ha reducido su recurrencia solo de
forma moderada y se asocia a frecuentes eventos adversos por lo que la búsqueda de nuevas estrategias
terapéuticas seguras y eficaces constituye una problemática actual. El policosanol, el extracto de semilla de
uva y la atorvastatina son sustancias con reportados efectos antiplaquetarios. Este estudio compara los
efectos preventivos del policosanol y el extracto de semilla de uva así como los efectos terapéuticos del
policosanol con la atorvastatina en un modelo de isquemia cerebral en Mongolian gerbils. Los resultados
mostraron que policosanol y extracto de semilla de uva presentaron eficacias similares para prevenir la
isquemia cerebral mientras que el policosanol y la atoravastatina resultaron similarmente efectivas para tratar
la isquemia cerebral previamente inducida en Mongolian gerbils.
Key words: policosanol, atorvastatin, ischemia-reperfusion
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-O-002 Comparison of policosanol and grape seed extract on global cerebral isquemia in
Mongolian gerbils
Molina Cuevas, Vivian; Ravelo Calzado, Yazmin; Mena Valdés, Licet; Noa Puig, Miriam; Pérez Guerra,
Yohani; Oyarzábal Year, Ambar; Mas Ferreiro, Rosa María; Valle Clara, Maikel; Mendoza Hernández, Nilda;
Jiménez Despaigne, Sonia
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
El ictus isquémico constituye la tercera causa de muerte y la primera de discapacidad en la población
adulta. El uso de agentes antiplaquetarios en el tratamiento del ictus isquémico ha reducido su recurrencia
solo de forma moderada y se asocia a frecuentes eventos adversos por lo que la búsqueda de nuevas
estrategias terapéuticas seguras y eficaces constituye una problemática actual. El ´policosanol, el
extracto de semilla de uva y la atorvastatina son sustancias con reportados efectos antiplaquetarios. Este
estudio compara los efectos preventivos del policosanol y el extracto de semilla de uva así como los
efectos terapéuticos del policosanol con la atorvastatina en un modelo de isquemia cerebral en Mongolian
gerbils. Los resultados mostraron que policosanol y extracto de semilla de uva presentaron eficacias
similares para prevenir la isquemia cerebral mientras que el policosanol y la atoravastatina resultaron
similarmente efectivas para tratar la isquemia cerebral previamente inducida en Mongolian gerbils.
Key words: policosanol, grape seed extract, ischemia-reperfusion
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-O-005
Extraction of phenolic compounds with antioxidant activity of Champa
(Campomanesia lineatifolia)
Otálvaro Álvarez, Ángela María; Chaparro González, María Patricia; Pabon Baquero, Ludy Cristina;
Rendón Fernández, Margarita Rosa; Muñoz Cadena, William Armando; Chávez Riaño, William Patricio
Universidad de La Salle, Colombia
[email protected]
Champa (Campumanesialineatifolia), a fruit with high potential to be used as a treatment of chronical
diseases
due to its chemical composition,
grown
the municipality
Miraflores
(Boyaca,
Colombia).
Champa (Campumanesialineatifolia),
a fruit iswith
high inpotential
to be usedof as
a treatment
of chronical
Nowadays,
post-harvest
losses
reach 97%,isbeing
an opportunity
value and
to giveColombia).
sustainability
diseases due
to its chemical
composition,
grownthis
in the
municipalitytoofadd
Miraflores
(Boyaca,
to
this crop.post-harvest
The aim of this
work
was97%,
to extract
Champa's
phenolic tocompounds
andtotogive
assess its antiNowadays,
losses
reach
being this
an opportunity
add value and
oxidant
capacity.
Lyophilized
subjected
to to
a extract
solid-liquid
extraction
(1:10),compounds
with water,and
ethanol
sustainability
to this
crop. The fruit
aim was
of this
work was
Champa's
phenolic
to and
ethanol-water
mixtures capacity.
(3:7) as solvents
at three
temperature
20, 50 y 70°C.
Phenolic
content
assess its anti-oxidant
Lyophilized
fruit was
subjectedlevels,
to a solid-liquid
extraction
(1:10),
with and
anti-oxidant
activity
were determined
for the(3:7)
dry as
extracts.
Extraction
ranged levels,
from 26,85%
50,07%,
water, ethanol
and ethanol-water
mixtures
solvents
at three yields
temperature
20, 50 yto70°C.
were
the content
higher numbers
were obtained
with water
as a solvent.
At extracts.
tested conditions,
Phenolic
and anti-oxidant
activity were
determined
for the dry
Extractionwater
yieldsshowed
ranged no
significant
dependency
extraction
temperature.
Phenolic
content
increased
extraction
temperature,
from 26,85%
to 50,07%,on
were
the higher
numbers were
obtained
with
water aswith
a solvent.
At tested
in
the rangewater
of 1469,32
± 152,14
to 5272,51
± 424,89 on
µg extraction
of galic acid/g
of lyophilized
pulp, content
where the higher
conditions,
showed
no significant
dependency
temperature.
Phenolic
figures
were
obtained
withtemperature,
ethanol-water
mix range
(7:3). of
Antioxidant
activity to
were
found ±between
20,44
± 2,57
increased
with
extraction
in the
1469,32
± 152,14
5272,51
424,89 µg
of galic
to
51,01
1,93 mM pulp,
TEAC/g
lyophilized
pulp,
beingwere
the water
andwith
the ethanol-water mix (7:3).
(7:3) the
acid/g
of ±lyophilized
where
the higher
figures
obtained
Anti-solvents
with
higher
values.
significant
effect
on activity
was
observed
temperature,
different
oxidant
activity
wereAfound
between
20,44
± 2,57 to
51,01
± 1,93 with
mM extraction
TEAC/g lyophilized
pulp,with
being
the
trends
depending
on the mix
solvent
used.
As with
a result
of this A research,
It is on
concluded
water and
the ethanol-water
(7:3) the
solvents
higher values.
significant effect
activity wasthat
Campumanesialineatifolia
is a natural with
product
with trends
a high depending
content of on
bio-active
compounds,
a great
observed with extraction temperature,
different
the solvent
used. As awith
result
of
potential
to be Itused
in agricultural,
pharmaceutical and cosmetic
industries.
this research,
is concluded
that Campumanesialineatifolia
is a natural
product with a high content of
bio-active compounds, with a great potential to be used in agricultural, pharmaceutical and cosmetic
Key words: campumanesialineatifolia, dpph, antioxidants, natural products
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-O-006
Hypoglycemic and Hypolipidemic effects of aqueous extract of "Estrella Dorada" in
Experimentally Induced Diabetic Rats
Álvarez Contreras, Carolina; Martínez Jorquera, Rolando
Universidad Austral de Chile, Chile
[email protected]
The present study investigates the hypoglycemic and hypolipidemic effect of oral administration of
queousextract
of "Estrella dorada" a endemic bush
corresponding to
of the Astaraceae
that
Thepresentstudyinvestigatesthehypoglycemicand
hypolipidemiceffect
of the
oral family
administration
of
grows
in the South
of Chile.dorada"
In this a
study
36 Malehypolipidemiceffect
Sprague-Dawley rats,
body
of 250-300
gr were
aqueousextract
of "Estrella
endemicbushcorrespondingtothefamily
of weight
theAstaraceaethatgrows
Thepresentstudyinvestigatesthehypoglycemicand
of oral
administration
of
divided
into 4ofgroups.
Diabetes
wasa induced
by three intra peritoneal injection
of 75 mg/kg Alloxan. Four
in the South
Chile.
aqueousextract
of "Estrella
dorada"
endemicbushcorrespondingtothefamily
of theAstaraceaethatgrows
weeks
after
the
first
injection,
the
rats
were
fasted
and
blood
glucose
levels
were
recorded.
Animals
were
In
thisstudy
36
MaleSprague-Dawleyrats,
bodyweight
of
250-300
gr
weredividedinto
4 groups.
Diabetes
in the South of Chile.
considered
diabeticperitoneal
when plasma
glucose
higher than 200
mg/dl. Thee
ffect of
wasinducedbythreeintra
injection
of 75concentrations
mg/kg
Alloxan.were
Fourweeksafterthefirstinjection,
In
thisstudy to
36 be
MaleSprague-Dawleyrats,
bodyweight
of 250-300
gr
weredividedinto
4 groups.
Diabetes
the
extract on the and
fast bloodglucoselevelswererecorded.
ingserum
triglycerides
levels, Lipidbeperoxides
(TBAS) in
theratswerefasted
Animalswereconsideredto
diabeticwhen
wasinducedbythreeintra
peritonealglucose,
injectiontotal
of 75colesterol,
mg/kg Alloxan.
Fourweeksafterthefirstinjection,
plasma and
in other
biochemical
parameters
after
a ten week
are expressed
glucoseconcentrationswerehighert
han analyzed
200 mg/dl.
Theeffect
of treatment. Results
theratswerefasted
and
bloodglucoselevelswererecorded.
Animalswereconsideredto
be diabeticwhen
as
meanglucoseconcentrationswerehighert
± SD. The data were analyzed
using
of Dunet
<0.05). The (TBAS)
infusion insupply to
theextractonthefastingserumglucose,
totalhan
colesterol,
triglycerideslevels,
Lipidperoxides
plasma
200multipleTest
mg/dl.
Theeffect
of (P
diabetic
rats
decreased
the
blood
glucose
level(284.6±31.2
to
170.32±6.3
mg/dl)
and
values
similar
plasmaand
in
otherbiochemicalparametersanalyzedafter
a
ten
weektreatment.
Results
are
expressed
theextractonthefastingserumglucose, total colesterol, triglycerideslevels, Lipidperoxides (TBAS)
in toasthe
observed
inin
control
ratswereanalyzedusingmultipleTest
were found (166.7±22.7 mg/dl).ofTreatment
mean ± SD.
The
data
Dunet
(P showed
<0.05). significantly
plasmaand
otherbiochemicalparametersanalyzedafter
a
ten weektreatment.
Results hypolipidemic
are expressedeffect
as
and
in ±other
parameters analyzed (LDL, HDL
y TGA).
the
fasting plasma
cholestero
Theinfusionsupplytodiabeticratsdecreasedthebloodglucoselevel(284.6±31.
170.32±6.3
mg/dl)
and
mean
SD. biochemical
The data wereanalyzedusingmultipleTest
of Dunet
(P Results
<0.05). 2onto
lwere
with untreated
and treatment normal (166.7±22.7
rats (142.9 ±mg/dl).
3.2 and
108.3
± 1.7 mg/dl),
well as
Theinfusionsupplytodiabeticratsdecreasedthebloodglucoselevel(284.6±31.
values compared
similar totheobserved
in controlratswerefound
2 to
170.32±6.3
mg/dl)asand
indiabetic
control
and diabeticintreated
rats(140.6 ±and
12.4(166.7±22.7
111.0 ±mg/dl).
1.7 mg/dl). The data demonstrated
that
values
Treatmentshowedsignificantlyhypolipidemiceffect
similar
totheobserved
controlratswerefound
inand
otherbiochemicalparametersanalyzed
(LDL, HDL
the
aqueous
extract of "Estrelladorada"
was associated
with antidiabetic and hypolipidemic
effects
on
the
Treatmentshowedsignificantlyhypolipidemiceffect
y TGA).
Resultsonthefasting
plasma cholesterolwerecomparedwithuntreatedand
and in otherbiochemicalparametersanalyzed
treatmentnormal
(LDL,
rats
HDL
yexperimentally
(142.9
TGA).±Resultsonthefasting
3.2 andinduced
108.3 diabetic
± 1.7plasma
mg/dl),
rats.cholesterolwerecomparedwithuntreatedand
as well as indiabetic control and diabetictreatedrats(140.6
treatmentnormal
± 12.4
rats and
111.0 ±± 1.7
(142.9
3.2 and 108.3 ± 1.7 mg/dl), as well as indiabetic control and diabetictreatedrats(140.6 ± 12.4 and
111 0 1 7
/dl)
Key words: hypoglycemic, hypolipidemic, diabetic rats
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-O-007
Preliminary phytochemical analysis, antioxidant activity and inhibiting the
angiotensin-converting enzyme (ACE) of the ethanol extract of the leaves of
Passiflora quadrangularis L.
Bareño, Lesly L.; Guerra C., Carlos M.; Guerrero, Mario F.; Jiménez, Ángel A.
Universidad Nacional de Colombia, Colombia
[email protected]
Oxidative stress and
and hypertension
hypertension are
are considered
considered factors
factorsprecursors
precursors ofofcardiovascular
cardiovasculardiseases
diseasesaccording
according
to
WHO are
to the
WHO
leading
are the
cause
leading
of death
causeworldwide,
of death worldwide,
treatment treatment
exposes the
exposes
patientthe
to patient
the continued
to the use of
continued
drugs
thatuse
leadof to
drugs
sidethat
effects,
lead totherefore
side effects,
it is therefore
required itnew
is required
therapeutic
new therapeutic
sources that
sources
decrease.
that The
decrease. The
Passiflora
genus
Passiflora
comprises
genus
about
comprises
500 species
aboutthat
500have
species
widethat
use
have
in folk
widemedicine
use in folk
in medicine
many countries;
in
many countries;
including
the species
including
Passiflora
the species
quadrangularis
PassifloraLinn,
quadrangularis
properties are
Linn,
attributed
properties
to headache,
are attributed
hypertension,
to
headache,
and
diabetes
hypertension,
(Dhawan, K.,
andDhawan,
diabetes S
(Dhawan,
& Sharma,
K., Dhawan,
2004). According
S & Sharma,
to the
2004).
above
According
in this to
study
the ACE
above in this
inhibitory
activity
study
in ACE
vitro by
inhibitory
the method
activity
of Simonetta
in vitro by the
Ronca
method
Testoni
of Simonetta
(Guerra, C;
Ronca
Peñalosa,
Testoni
G; (Guerra,
Mendez, C;
J&
Peñalosa,
Murillo,
W.G;
2014),
Mendez,
antioxidant
J & Murillo,
activity
W.by2014),
ABTSantioxidant
and preliminary
activity
phytochemical
by ABTS andanalysis
preliminary
(AFP)
phytochemical
in the ethanol
analysisof(AFP)
extract
the leaves
in the of
ethanol
P. quadrangularis
extract of thewas
leaves
evaluated,
of P. quadrangularis
which will establish
was evaluated,
preform which
if it haswill
effects on
establish preform
hypertension.
Regarding
if it has the
effects
ACEoninhibitory
hypertension.
activityRegarding
was established
the ACE
that
inhibitory
the extract
activity
at awas
concentration
establishedof
that the
0.05
mgextract
/ ml inhibited
at a concentration
70%, whereas
of
of 0.05
0.05
the pattern
mg
mg //ml
ml(Captopril)
inhibited
inhibited 70%,
70%,
to 1.0whereas
whereas
mg / mlthe
obtained
thepattern
pattern
only
(Captopril)
(Captopril)
15%; antioxidant
toto1.0
1.0mg
mg
activity
with ABTS
extract
behaved activity
similarlywith
to the
reference
with
the PPA
also possible
to
ml
only
antioxidant
similarly
to the
thewas
reference
standard,
mlobtained
obtained
only 15%;
15%;
antioxidant
activity
ABTS
extractstandard,
behaved similarly
to
reference
standard,
establish
the presence
of saponins,
flavonoids
alkaloids,
of which flavonoids
its effect onand
hypertension
is which
known
with the PPA
was also possible
to establish
theand
presence
of saponins,
alkaloids, of
(Rojas, 2009). According to the results it follows that the extract has hypotensive effect, which must be
confirmed with in vivo studies.
Key words: passiflora quadrangularis l, antioxidant activity, hypertension, in vitro, preliminary
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-O-009 Cymbopogon citratus aqueous fraction do not protect against UVC damage in
Caulobacter crescentus
León Fuentes, Fabiana; García Fernández, F.; González Pumariega, M.; Sánchez Lamar, Ángel
Universidad de La Habana (UH), Cuba
[email protected]
Ozone layer hole entails ultraviolet radiation increase on the earth surface, raising the risk of skin diseases,
even
Theentails
use ofultraviolet
vegetal compounds
is a new
strategy
protectraising
peoplethe
against
Ozonecancer.
layer hole
radiation increase
on the
earth to
surface,
risk of UV
skindamages .
Substances
obtained
natural
sources
have a huge
molecular
and
biological
diseases, even
cancer.from
The use
of vegetal
compounds
is a new
strategydiversity
to protect
people
againstfunctions.
UV
However,
evaluationobtained
of the toxic
these compounds
required before
its beneficial
properties
damages. the
Substances
fromcapacity
natural of
sources
have a hugeismolecular
diversity
and biological
can
be used.
Cymbopogon
citratus
(DC)toxic
Stapf
is consumed
as a popularis decoction
and has
functions.
However,
the evaluation
of the
capacity
of these compounds
required before
its several
properties
such as: anticancer,
antitumor
and antimutagenic.
of consumed
these studies
assayed
in total
beneficial properties
can be used.
Cymbopogon
citratus (DC) Many
Stapf is
as awere
popular
decoction
extract,
a less
portionsuch
was as:
assayed
in fractioned
extract.
In this work, we
tested
the toxicity
the
Many
of these
studiesand
were
and has while
several
properties
anticancer,
antitumor
and antimutagenic.
ability
to protect
against while
UVC light
of portion
the aqueous
fractionsinobtained
Cymbopogon
citratus.
To dothe
so,
assayed
in total extract,
a less
was assayed
fractionedfrom
extract.
In this work,
we tested
SOS
Chromotest,
RifRtoand
Survival
testUVC
werelight
used
in Caulobacter
crescentus.
Increasing
concentrations
toxicity
and the ability
protect
against
of the
aqueous fractions
obtained
from Cymbopogon
of
these To
fractions
(0.1, 0.5,
1, 2 andRifR
4 mg/mL)
were applied
continuously:
before, during
and after UVC
citratus.
do so, SOS
Chromotest,
and Survival
test were
used in Caulobacter
crescentus.
exposure
J/m2). Results
showed
that this
fraction
not4cytotoxic
and don´t
possess
any genotoxic
Increasing(45
concentrations
of these
fractions
(0.1,
0.5, 1,was
2 and
mg/mL) were
applied
continuously:
properties.
Aqueous
fraction
decrease
the induction
of SOS phenomenon,
survival
and
frequency
before, during
and after
UVC can´t
exposure
(45 J/m2).
Results showed
that this fraction
was not
cytotoxic
andof
mutations
in irradiated
cells. Finally,
the aqueous
Cymbopogon
(DC) Stapf,
do not protect
don´t possess
any genotoxic
properties.
Aqueousfraction
fractionofcan´t
decreasecitratus
the induction
of SOS
phenomenon,
DNA
from primarily
survival
damage
and frequency
induced by
of mutations
UVC light. in irradiated cells. Finally, the aqueous fraction of
Key words: cymbopogon citratus, aqueous fractions, sos chromotest, survival test, rifr test, caulobacter
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-O-010 Comparation of antioxidants properties and polyphenols content of aquoeus extract
from seaweeds Bryothamniontriquetrum and Halimedaopuntia
Díaz Gutiérrez, Daylín; Méndez Ortega, Wendy; de Oliveira e Silva, Ana Mara; Zaldívar Muñoz,
Claudina; Mancini Filho, Jorge; Vidal Novoa, Alexis
Universidad de La Habana (UH), Cuba
[email protected]
The current investigation focuses attention on the antioxidant properties of aqueous extracts of marine
algae
Bryothamnion
triquetrum
Halimeda
opuntia,
and the
relationship
of suchextracts
properties
with total
The current
investigation
focusesand
attention
on the
antioxidant
properties
of aqueous
of marine
polyphenol
content,
using
different
experimental
in
vitro
antioxidant
assessment
models.
We
observed
algae Bryothamnion triquetrum and Halimeda opuntia, and the relationship of such properties with total
following
Reducing
power experimental
(concentration
128 antioxidant
mg/mL, B. assessment
triquetrum OD=2.798
and
H. opuntia
polyphenolresults:
content,
using different
in vitro
models. We
observed
OD=0.800,
ascorbic
acid with
1 mg/mL
OD=2,824),
assays(B.
triquetrum
IC50=1.15
0.06 and H.
following results:
Reducing
power
(concentration
128DPPH
mg/mL,
B. triquetrum
OD=2.798
and H.± opuntia
opuntia
IC50=12.34
0.30
mg/mL),
inhibition
of lipidDPPH
peroxidation
in rat
brain homogenates
triquetrum
OD=0.800,
ascorbic ±acid
with
1 mg/mL
OD=2,824),
assays(B.
triquetrum
IC50=1.15 ±(B.
0.06
and H.
CI50=5.09±
025
and
H.
opuntiaIC50=1.25±
031
mg/mL)
and
the
inhibition
of
red
blood
cell
(RBC)
opuntia IC50=12.34 ± 0.30 mg/mL), inhibition of lipid peroxidation in rat brain homogenates (B. triquetrum
haemolysis
induced
by
AAPH
(with
5
mg/mL
B.
triquetrum
inhibited
until
100
%
and
H.
opuntia
24%).
The
CI50=5.09± 025 and H. opuntiaIC50=1.25± 031 mg/mL) and the inhibition of red blood cell (RBC)
contents
of induced
polyphenolic
compounds
was forB.B.triquetrum
triquetruminhibited
51,21 ±until
2,25100
and% H.
opuntia
19,9924%).
± 1,12
µg
haemolysis
by AAPH
(with 5 mg/mL
and
H. opuntia
The
GAE/
mgof
extract.
It was demonstrated
thatforB.triquetrum
extract
more
effective
than 19,99
H. opuntia
free
contents
polyphenolic
compounds was
B. triquetrum
51,21was
± 2,25
and
H. opuntia
± 1,12inµg
radical
scavenge
reducing
power while
H.opuntia was
more
effective
in inhibition
peroxidation
GAE/ mg
extract. Itand
was
demonstrated
that B.triquetrum
extract
was
more effective
thanofH.lipid
opuntia
in free
in
rat brain
homogenatesand
inhibition
of red blood
(RBC)
haemolysis
induced
AAPH.Both
radical
scavenge
and reducing the
power
while H.opuntia
was cell
more
effective
in inhibition
of lipidbyperoxidation
extractswerenottoxicin
the model
ofacute oftoxicity
ofArtemiasalina.
These resultssupporting
itspossible
in rat brain homogenatesand
red blood
cell (RBC) haemolysis
induced by AAPH.Both
the inhibition
application
asphytodrugand
/ornutraceuticalfor
its
antioxidant
propertiesand
low
toxicity.
extractswerenottoxicin the model ofacute ossible
Key words: seaweed, natural antioxidants, halimeda opuntia, bryothamnion triquetrum, polyphenol
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-O-011 Structural identification of obtained compounds from the ethanolic extract of
Dioscorea bartlettii Morto
Ceja Ochoa, Irais Araceli; García Vicencio, Miguel; Martínez Rocha, Alejandra; Sánchez López, Abigail
Cristal; Berdeja Martínez, Blanca Margarita; Fregoso Aguilar, Tomás Alejandro
Instituto Politécnico Nacional, México
[email protected]
Los productos
productos de
de origen
origen vegetal
vegetalson
sonrecursos
recursosrenovables
renovablesdede
múltiples
usos
el hombre,
múltiples
usos
parapara
el hombre,
entreentre
los los
cuales se
se encuentran
encuentran el
el uso
usomedicinal
medicinaly yeleltoxicológico.
toxicológico.
información
obtenida
la investigación
LaLa
información
obtenida
de de
la investigación
de de
compuestos de origen
origen vegetal
vegetal ayuda
ayuda aacomprender
comprender lalafisiología
fisiologíay ybioquímica
bioquímicadedeloslosorganismos
organismosque
queloslos
producen y poder lograr
lograr su
su mejor
mejor aprovechamient
aprovechamientoo desde
desde elelpunto
punto de
devista
vistacientífico,
científico,médico
médicoy yeconómico.
Dentro
de la composición química de los productos vegetales se pueden considerar dos grupos: Productos
económico.
del
metabolismo
primarioquímica
y Productos
del metabolismo
secundario.
que destacar
que muchos
Dentro
de la composición
de los productos
vegetales
se pueden Hay
considerar
dos grupos:
metabolitos
respuestas
fisiológicas secundario.
muy específicas, en muchos casos
Productos delsecundarios
metabolismodesencadenan
primario y Productos
del metabolismo
uniéndose
a determinados
receptores
con secundarios
una destacable
complementariedad.
hecho proporciona
Hay que destacar
que muchos
metabolitos
desencadenan
respuestasEste
fisiológicas
muy
un
importante
y justificación
para
el estudio de nuevos
productos
de origen animal o
específicas,
enincentivo
muchos casos
uniéndose
a determinados
receptores
con unanaturales
destacable
vegetal,
como lo son Este
los compuestos
que se puedan
encontrar
en la planta
de estudio:
complementariedad.
hecho proporciona
un importante
incentivo
y justificación
paraDioscorea
el estudiobartlettii
de
Morton.
El objetivo naturales
del trabajodeesorigen
separar
y purificar,
en la
medida
quelos
sea
posible, losque
compuestos
nuevos productos
animal
o vegetal,
como
lo son
compuestos
se puedanque se
encuentran
hojasdede
Dioscorea
bartlettii
MortonMorton.
para posteriormente tratar de elucidar su estructura
encontrar enenlalas
planta
estudio:
Dioscorea
bartlettii
mediante
de infrarrojo
y de resonancia
magnética
elucidación
de
El objetivoespectroscopia
del trabajo es separar
y purificar,
en la medida
que sea nuclear.
posible, La
los separación
compuestosy que
se
dichos
compuestos
nos llevó
a la conclusión
de Morton
que unopara
de los
componentes tratar
que más
fácilmente
encuentran
en las hojas
de Dioscorea
bartlettii
posteriormente
de elucidar
su se separó
de
dicha planta
fue espectroscopia
una saponina. La
contiene
también otros
metabolitos
estructura
mediante
de planta
infrarrojo
y de resonancia
magnética
nuclear.pero no se separaron
La separación y elucidación de dichos compuestos nos llevó a la conclusión de que uno de los
fácilmente.
Key words: dioscorea, separación, metabolitos secundarios, saponina
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-O-012 Influence of operating conditions on the extraction of polyphenols from Moringa
oleifera Lam leaves
Valdés Hernández, Geidy Vivian; Comet Rodríguez, Raidel; Cruz Viera, Luis
Instituto Superior Politécnico José Antonio Echeverría (Cujae), Cuba
[email protected]
Polyphenols are secondary metabolites present in plants, which have gained popularity for its wide
antioxidant
Numerous
investigations
have
shown
that
the gained
leaves popularity
of Moringaforoleifera
Polyphenolsproperties.
are secondary
metabolites
present in
plants,
which
have
its wideLam are
rich
in this type
of substance.
The investigations
aim of the present
is that
to study
the influence
of operating
conditions
antioxidant
properties.
Numerous
have work
shown
the leaves
of Moringa
oleifera Lam
are
on
extraction
polyphenols
solid-liquid
contactwork
fromisthe
of this plant.
The experimental
richthe
in this
type ofofsubstance.
Thebyaim
of the present
to dried
study leaves
the influence
of operating
conditions
design
consideredofthe
type of solvent
(ethanoliccontact
solutions
different
concentrations),
temperature
(50, 60
on the extraction
polyphenols
by solid-liquid
fromatthe
dried leaves
of this plant.
The
and
70°C) and
the considered
solute-solvent
and(ethanolic
1:35 m/v)
ratio. The
extraction
kinetics were also
experimental
design
the (1:15,
type of1:25
solvent
solutions
at different
concentrations),
evaluated.
investigated
temperatures,
the activation
energy
the m/v)
process
was
the solute-solvent
(1:15, 1:25
andof1:35
ratio.
Thedetermined.
extraction From
temperatureFrom
(50, the
60 and
70°C) and
the
above
conditions,
those that
reported
the best results
were ethanol
solutionenergy
to 60%
as solvent,
the investigated
temperatures,
the activation
of (v/v)
the process
kinetics
were
also evaluated.
From
solute-solvent
ratio
1:35
with
an extraction
30 minutes.
Forresults
these were
conditions,
polyphenol
ethanola solution
to
was determined.
From
the(m/v)
above
conditions,
thosetime
that of
reported
the best
content
17,5
mg gallic
acid equivalent/g
dry(m/v)
masswith
withanantioxidant
mg Trolox
equivalent / g
60% (v/v)of as
solvent,
solute-solvent
ratio 1:35
extraction activity
time of of
30 35
minutes.
For these
dry
mass, was
obtained. The
temperature
effect
significant in
investigated
range. The
nature
of
conditions,
a polyphenol
content
of 17,5 mg
gallicwas
acidnot
equivalent/g
drythe
mass
with antioxidant
activity
of 35
mg leaching
the
Trolox equivalent/g
is physicaldry
considering
that
the activation
energy was effect
11,47was
± 0,96
mass, was
obtained.
The temperature
not kJ/mol.
significant in the
investigated range. The nature of the leaching is physical considering that the activation energy was
Key words: moringa, total polyphenols content, extraction kinetics, antioxidant activity
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-O-013 In vitro evaluation of three biological agents against ticks Otobius megnini y
Rhipicephalus (Boophilus) microplus in the state of Mexico
Gayón Amaro, Susana Gabriela; Beltrán León, Trinidad; Castelán Ortega, Octavio A.; Velasco Galindo,
Edelmira
México
[email protected]
Biological control is a tactic to regulate plague populations through the use of natural elements. Tick
infestationcontrol
affects isany
animal
important
economical
In México,
Biological
a tactic
to species
regulate with
plague
populations
throughand
thehealth
use ofrepercussions;
natural elements.
Tick losts
about 48 millions
dollars
per
year had
estimated
becauseand
of this
parasitosis
in cattle;Inthat
is why the
infestation
affects of
any
animal
species
withbeen
important
economical
health
repercussions;
Mexico,
main
purpose
of
this
research
was
to
evaluate
the
in-vitro
effect
of
two
organics
and
a
microbial
substances
losts about 48 millions of dollars per year had been estimated because of this parasitosis in cattle; that is
in the
of adults
Otobius was
megnini
y Rhipicephalus
150organics
adult ticks
genres
why
theviability
main purpose
of ticks
this research
to evaluate
the in-vitromicroplus.
effect of two
andofa each
microbial
were collected
from
bovines;
through
those
15 groups
with
10 subjects were
made to 150
prove
three
biological
substances
in the
viability
of adults
ticks
Otobius
megnini
y Rhipicephalus
microplus.
adult
ticks
of
agents:
essential
of Juniperus
communis,
metanolic
of Annona
purified
each
genres
were oil
collected
from bovines;
through
thoseextract
15 groups
with 10muricata
subjectsseeds,
were made
to spore
prove of
M. anisopliae
andessential
distillatedoilwater
with tween
80 to 0.1%
for control
groups
(3) in four
different
levels
three
biologicalMa14
agents:
of Juniperus
communis,
metanolic
extract
of Annona
muricata
seeds,
of concentration
using
the immersion
method.
The
results
15 daily
observations
purified
spore of M.
anisopliae
Ma14 and
distillated
water
withwere
tweenestablished
80 to 0.1% after
for control
groups
(3) in
through
a stereoscopic
microscopyusing
getting
maximum
mortality
ratings
of 50% after
and 100%
four
different
levels of concentration
the minimum
immersionand
method.
The results
were
established
15
respectively.
According
those resultsmicroscopy
it is possible
to design
integral
plague management
systems
daily
observations
throughwith
a stereoscopic
getting
minimum
and maximum
mortality ratings
of
which
consider
natural elements
like thewith
entomopathogenic
Metarhizium
anisopliae
Ma14, soursop
50%
and
100% respectively.
According
those results it isfungus
possible
to design integral
plague
seeds (Annona
muricata)
andconsider
pinus (Juniperus
communis)
agents with ixodicide
potential
against adult
management
systems
which
natural elements
like like
the entomopathogenic
fungus
Metarhizium
ticks of theMa14,
genressoursop
Rhipicephalus
microplus
y Otobius
anisopliae
seeds (Annona
muricata)
andmegnini.
pinus (Juniperus communis) like agents with
Key words: veterinary medicine, biological control, ectoparasites, ticks
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-O-014
Antiinflamatory effects of Abexol (D-002)
Molina Cuevas, Vivian; Ravelo Calzado, Yazmin; Mas Ferreiro, Rosa María; Zamora Rodríguez, Zullyt
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
La inflamación constituye un factor etiológico importante en el desarrollo de enfermedades crónicas como
la osteoartritis, el cáncer, la hiperplasia prostática benigna, entre otras. Los anti-inflamatorios no
esteroidales (AINEs) son medicamentos muy efectivos empleados para tratar la inflamación aguda,
crónica y el dolor asociado; sin embargo su uso constituye una de las causas principales de úlcera
gástrica. Por tal motivo la búsqueda de nuevas sustancias anti-inflamatorios con buen perfil de seguridad
gastrointestinal constituye una problemática actual. Este trabajo evaluó los efectos del D-002, mezcla de
seis alcoholes alifáticos de alto peso molecular obtenida de la cera de abejas, en modelos experimentales
de inflamación y úlcera gástrica así como su posible gastrotoxicidad. La administración oral con D-002
en roedores redujo de forma significativa y moderada la inflamación en diferentes modelos
experimentales, mientras que a diferencia de los AINES no produjo gastrotoxicidad y por el contrario
protegió la mucosa gástrica.
Key words: Abexol, antiinflamatory effect, drug, natural product
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-O-015
Gastrotoxic and gastroprotector effects of Abexol (D-002)
Molina Cuevas, Vivian, Ravelo Calzado, Yazmin; Mas Ferreiro, Rosa María; Zamora Rodríguez, Zullyt
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
La inflamación constituye un factor etiológico importante en el desarrollo de enfermedades crónicas como la
osteoartritis, el cáncer, la hiperplasia prostática benigna, entre otras. Los anti-inflamatorios no esteroidales
(AINEs) son medicamentos muy efectivos empleados para tratar la inflamación aguda, crónica y el dolor
asociado; sin embargo su uso constituye una de las causas principales de úlcera gástrica. Por tal motivo la
búsqueda de nuevas sustancias anti-inflamatorios con buen perfil de seguridad gastrointestinal constituye
una problemática actual. Este trabajo evaluó los efectos del D-002, mezcla de seis alcoholes alifáticos de
alto peso molecular obtenida de la cera de abejas, en modelos experimentales de inflamación y úlcera
gástrica así como su posible gastrotoxicidad. La administración oral con D-002 en roedores redujo de forma
significativa y moderada la inflamación en diferentes modelos experimentales, mientras que a diferencia de
los AINES no produjo gastrotoxicidad y por el contrario protegió la mucosa gástrica.
Key words: Gastrotoxic, gastroprotector effects, Abexol D-002
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-O-016
Effects of Abexol (D -002) on esophagitis induced by gastroesophageal reflux in rats
Zamora Rodríguez, Zullyt; Molina Cuevas, Vivian; Ravelo Calzado, Yazmin; Mas Ferreiro, Rosa María;
Pérez Guerra, Yohani; Oyarzábal Year, Ambar; Jiménez Despaigne, Sonia
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
La enfermedad de reflujo gastroesofágico (ERGE), es una
una patología
patología crónica, donde
donde alrededor
alrededor del
del 40
40 %
% de
la
mundial
la sufre.
La ineficacia
de los
concon
inhibidores
de la
de protones
y la
depoblación
la población
mundial
la sufre.
La ineficacia
de tratamiento
los tratamiento
inhibidores
debomba
la bomba
de protones
aparición
de reacciones
adversas,
hacen
que que
sea sea
esteeste
hecho
una una
problemática
de salud
en laenactualidad.
y la aparición
de reacciones
adversas,
hacen
hecho
problemática
de salud
la
Con
el objetivo
evaluar de
el efecto
del D-002 del
en D-002
diferentes
modelos experimentales
actualidad.
Con de
el objetivo
evaluarprotector
el efectoesofágico
protector esofágico
en diferentes
modelos
de
esofagitis, sedeemplearon
estudio ratas
macho,
a las
seales
aguda,
experimentales
esofagitis,en
seelemplearon
en elSD
estudio
ratas
SDcuales
macho,
lasindujo,
cualeslaseesofagitis
les indujo,
la
en
la cual aguda,
se evaluó
efecto
citoprotector
5 hcitoprotector
después de 5lahinducción;
el la
modelo
de esofagitis
esofagitis
en laelcual
se evaluó
el efecto
después de
inducción;
el modelogastrode
duodeno-esofágico,
el que fue evaluado
48fue
h después
induccióndey la
el inducción
modelo de
reflujo
esófagoesofagitis gastro-duodeno-esofágico,
el que
evaluado de
48 la
h después
y el
modelo
de
duodenal
con gastrectomía
con una duración
14 días
operatorio.
Los resultados
reflujo esófago-duodenal
con gastrectomía
con unamáxima
duracióndemáxima
de post
14 días
post operatorio.
Los
demostraron
el efecto protector
del D-002 del
en D-002
los modelos
experimentales,
con excepción
resultados demostraron
el efecto esofágico
protector esofágico
en los modelos
experimentales,
con del
modelo
de del
reflujo-gastro-duodeno-esofágico.
Dicho efecto protector
estuvo
mediadoestuvo
por lamediado
inhibiciónpor
delala
excepción
modelo de reflujo-gastro-duodeno-esofágico.
Dicho efecto
protector
peroxidación
lipídica
y proteico
sin interferir
en sin
el volumen
la acidez
del ni
jugo
gástrico.
inhibición de la
peroxidación
lipídica
y proteico
interferir de
en secreción
el volumennide
secreción
la acidez
del Se
concluye,
que se
efecto
esófago protector
D-002 protector
en la esofagitis
inducida
reflujo gastrojugo gástrico.
Sedemostró
concluye,elque
se demostró
el efecto del
esófago
del D-002
en lapor
esofagitis
esofágicoy esófago-duodenal.
Key words: Abexol, D -002, esophagitis, gastroesophageal reflux, rats
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-O-018
Effects of Abexol in patient with osteoarthritis
Illnait Ferrer, José; Fernández Travieso, Julio César; Puente, Roberto; Mendoza Castaño, Sarahí; Mas
Ferreiro, Rosa María; Carbajal D., Daisy; Mesa, Meilis; Gámez Menéndez, Rafael; Suárez, Pablo
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
Background and purpose:
purpose: Osteoarthritis
Osteoarthritis (OA)
(OA)isisa adegenerative
degenerativejoint
jointdisease
disease
characterized
joint
pain
characterized
by by
joint
pain
and dysfunction caused by
by aa progressive
progressive and
and irreversible
irreversible loss
lossofofarticular
articularcartilage,
cartilage,that
thataffects
affectshundreds
hundreds
of millions
millionsworldwide,
worldwide,mainly
mainly
elderly.
The management
OA included
a combination
thethe
elderly.
The management
of OA of
included
a combination
of non- of nonpharmacological interventions and
and pharmacologic
pharmacologic agents.
agents. Non
Nonselective
selectivenon-steroidal
non-steroidalanti-inflammatory
anti-inflammatory
drugs
inhibitors provide
provide symptoms
symptoms relief
relief in
in OA,
OA, but
but produce
produce several
several adverse
adverse effects
effects
drugs and
and ciclooxygenase
ciclooxygenase 22 inhibitors
(AE),
which supports
supports the
the search
search for
for other
other options.
options. Abexol,
Abexol, aamixture
mixture of
of higher
higher aliphatic
aliphatic beeswax
beeswax alcohols,
alcohols,
(AE), which
has
effects experimentally
experimentally and
and clinically.
clinically. This
This double-blind
double-blind study
has been
been shown
shown to
to produce
produce anti-inflammatory
anti-inflammatory effects
investigated
the effects
of Abexol
(50-100
mg/day)
on patients
with OA
symptoms
randomized
to Abexol
study investigated
the effects
of Abexol
(50-100
mg/day)
on patients
with
OA symptoms
randomized
to or
placebo
for
6
weeks.
Methods:
The
primary
efficacy
outcome
was
the
reduction
of
the
total
Western
Ontario
Abexol or placebo for 6 weeks.
and
McMaster
Individual
Osteoarthritis
(WOMAC)
efficacyand
outcome
was the
Methods:
The primary
efficacy
outcome Index
was the
reductionscore.
of the The
totalsecondary
Western Ontario
McMaster
reduction
pain, joint stiffness,
physical activity
scores,
and the efficacy
reductionoutcome
of the VAS
The collateral
Individual on
Osteoarthritis
Index (WOMAC)
score. The
secondary
wasscores.
the reduction
on
efficacy
outcome
was
reduction
the use
of rescue
medications.
tolerability
assessed.
pain, joint
stiffness,
physical
activity
scores,
and the
reduction ofTreatment
the VAS scores.
The was
collateral
efficacy
Results:
study
completion
Abexol
(50-100
mg/day) significantly
thewas
total
WOMAC score versus
outcome At
was
reduction
the use
of rescue
medications.
Treatment reduced
tolerability
assessed.
baseline
(p<0.00001,
77.1%)
and
placebo
(p
<0.0001,
65.4%),
and
also
decreased
the pain (54.9%),
joint
Results: At study completion Abexol (50-100 mg/day) significantly reduced the total WOMAC
score
stiffness
(76.8%)(p<0.00001,
and physical77.1%)
activityand
(66.9%)
scores
versus placebo.
Afteralso
1 week
on therapy
all benefits
versus baseline
placebo
(p <0.0001,
65.4%), and
decreased
the pain
were
significant
already.
In addition,
Abexol
significantly
reduced
theplacebo.
VAS score
versus
baseline
(54.9%),
joint stiffness
(76.8%)
and physical
activity
(66.9%) scores
versus
After 1
week on
(p<0.00001,
60.2%)were
and placebo
(p already.
<0.0001,In46.8%).
use of
rescue medication
in Abexol
(6/30, 20%)
therapy all benefits
significant
addition,The
Abexol
significantly
reduced the
VAS score
was
lower
(p <0.01)
than in placebo
56.7%).(pTreatment
was well The
tolerated.
(2 Abexol,
versus
baseline
(p<0.00001,
60.2%)(17/30;
and placebo
<0.0001, 46.8%).
use ofSeven
rescuepatients
medication
in
5
placebo)
reported
moderate
AE.
Conclusions:
Abexol
(50-100
mg/day)
given
for
6
weeks
improved
Abexol (6/30, 20%) was lower (p <0.01) than in placebo (17/30; 56.7%). Treatment was well tolerated. OA
symptoms
and was
well tolerated.
Abexol
could
be a suitable
Seven patients
(2 Abexol,
5 placebo)
reported
moderate
AE. option for managing OA symptoms, but this
appreciation
extensive
further given
research.
Conclusions: requires
Abexol (50-100
mg/day)
for 6 weeks improved OA symptoms and was well tolerated.
Abexol could be a suitable option for managing OA symptoms, but this appreciation requires extensive
Key words: Abexol, osteoarthritis
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-O-019
Carcinogenicity of D-004
Gutiérrez Martínez, Ariadne; Gámez Menéndez, Rafael; Noa Puig, Miriam; Valle Clara, Maikel;
Mendoza Hernández, Nilda; Pérez Guerra, Yohani; Oyarzábal Year, Ambar; Bucarano, I.; Goicochea,
E.; Jiménez Despaigne, Sonia; García, H.
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
El D-004 es un
un extracto
extracto lipídico
lipídico del
del fruto
fruto de
delalaRoystonea
Roystonearegia
regia(palma
(palmareal),
real),constituido
constituidopor
poruna
unamezcla
mezcla
de ácidos
ácidos grasos,
grasos,principalmente
principalmenteoleico,
oleico,
palmítico,
láurico
y mirístico,
quemostrado
ha mostrado
prevenir
la
palmítico,
láurico
y mirístico,
que ha
prevenir
la
hiperplasia prostática inducida con testosterona, no con dihidrotestosterona,
dihidrotestosterona, en
en roedores.
roedores. El
El objetivo
objetivo del
del
presente estudio consistió en determinar si el tratamiento oral a largo plazo
plazo (24
(24 meses)
meses) con
con D-004
D-004 (500(5001500 mg/kg) muestra evidencias
evidencias de
de toxicidad
toxicidad y/o
y/ocarcinogenicidad
carcinogenicidad en
enratas
ratasSD
SDadultas
adultasjóvenes
jóvenesdedeambos
sexos.
animales
se distribuyeron
aleatoriamente
en cuatro
(50/sexo/grupo),
un grupo
ambos Los
sexos.
Los animales
se distribuyeron
aleatoriamente
en grupos
cuatro grupos
(50/sexo/grupo),
uncontrol
grupo
al
cual al
sólo
sesólo
le administró
el vehículo
Tween Tween
65/H2065/H20
y tres ygrupos
tratados
con D-004
a las a
dosis
control
cual
se le administró
el vehículo
tres grupos
tratados
con D-004
las de
500,
y 1500
tratamientos
se administraron
oralmenteoralmente
por intubación
gástrica una
vez al
dosis1000
de 500,
1000mg/kg.
y 1500Los
mg/kg.
Los tratamientos
se administraron
por intubación
gástrica
día
24 durante
meses. 24
La meses.
mortalidad
(análisis de(análisis
supervivencia),
tiempo de aparición
tumores,designos
una durante
vez al día
La mortalidad
de supervivencia),
tiempo dede
aparición
clínicos,
de alimentos,
hematológicos
y de bioquímicay de
sanguínea,
tumores, peso
signoscorporal,
clínicos,consumo
peso corporal,
consumoparámetros
de alimentos,
parámetros hematológicos
peso
de órganos
e incidencia
de lesiones
neoplásicas
y no neoplásicas
bioquímica
sanguínea,
peso de órganos
e incidencia
de lesiones
neoplásicasdiferencias
no
neoplásicas ynonomostraron
significativas
ni tendencias
con las dosis
entre animales
y controles
ambos ysexos.
Por tanto,
mostraron diferencias
significativas
ni tendencias
con lastratados
dosis entre
animalesentratados
controles
en
el
D-004sexos.
no incrementa
acelera la la
aparición
de lesiones
o malignas,
ambos
Por tanto,laelfrecuencia
D-004 no o
incrementa
frecuencia
o aceleraneoplásicas
la apariciónbenignas
de lesiones
ni
induce la benignas
aparición odemalignas,
tumores ni
inusuales
la especie,
muy
similar
la frecuencia de
neoplásicas
induce laaaparición
de comportándose
tumores inusuales
a la
especie,
tumores
entre los
grupos
tratados
y controles.
Los resultados
estudio
permiten
concluir Los
que el D-004
comportándose
muy
similar
la frecuencia
de tumores
entre losdel
grupos
tratados
y controles.
no
es tóxico
carcinogénico
en concluir
ratas SDque
tratadas
durante
meses
y que la dosisen
mayor
mg/kg)
resultados
delo estudio
permiten
el D-004
no es24
tóxico
o carcinogénico
ratas (1500
SD tratadas
se
comportó
como yelque
nivella donde
no se (1500
producen
efectos
tóxicos observables.
durante
24 meses
dosis mayor
mg/kg)
se comportó
como el nivel donde no se producen
Key words: Carcinogenicity, D-004
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-O-020
Effects of D-004 in patient with benign prostatic hyperplasia
Guzmán, Raúl
Hospital Salvador Allende ,, Cuba
[email protected]
Benign prostatic hyperplasia (BPH), common among elderly men, often causes lower urinary tract
symptoms
(LUTS).
BPH/LUTS
therapies
5- -reductase
inhibitors,
1Benign prostatic
hyperplasia
(BPH),pharmacological
common among elderly
men,include
often causes
lower urinary
tract
adrenoreceptors
blockers
and phytotherapies,
saw palmetto
lipid extracts.
D-004, a1-lipid extract of
symptoms (LUTS).
BPH/LUTS
pharmacologicallike
therapies
include(SP)
5- -reductase
inhibitors,
Roystonea
regia fruits,
reduced
experimental prostate
in rodents
and theD-004,
International
Prostate
adrenoreceptors
blockers
and phytotherapies,
like sawhyperplasia
palmetto (SP)
lipid extracts.
a lipid extract
Symptoms
Score
effectively
as SP in aprostate
pilot trialhyperplasia
in men withinBPH.
Thisand
work
two clinical
of Roystonea
regia(IPSS)
fruits,as
reduced
experimental
rodents
theincludes
International
trials
conducted
in order
compare
the effects as
of SP
D-004
with
BPH.
In the
phase II
Prostate
Symptoms
Scoreto(IPSS)
as effectively
in aand
pilotSP
trialininpatients
men with
BPH.
This
workfirst
includes
study
sixty-one
withinmoderate
BPH werethedouble-blindly
randomised
D-004 or
SPBPH.
(320 Inmg/day
two clinical
trialspatients
conducted
order to compare
effects of D-004
and SP intopatients
with
the
both)
capsules
for 4sixty-one
months. patients
Decrease
onmoderate
IPSS wasBPH
the primary
efficacy variable.
Effects on
prostate
first phase
II study
with
were double-blindly
randomised
to D-004
or size,
SP
residual
volume
post-voiding
and4 serum
prostate
specific
antigen
were secondary
outcomes.
Afteron2
(320 mg/day
both)
capsules for
months.
Decrease
on IPSS
was(PSA)
the primary
efficacy variable.
Effects
months
therapy,
D-004
and post-voiding
SP decreased
IPSS
valuesspecific
versus antigen
baseline(PSA)
and this
increased
prostateon
size,
residual
volume
andmean
serum
prostate
wereeffect
secondary
at
study completion,
whenonIPSS
decreased
36.0%
D-004 mean
and 34.1%
with SP,
without
differenc
outcomes.
After 2 months
therapy,
D-004 and
SP with
decreased
IPSS values
versus
baseline
and es
this effectgroups.
between
increased
Bothattreatments
study completion,
did not modify
when IPSS
prostate
decreased
size, residual
36.0%volume
with D-004
post- and
voiding.
34.1%
Twowith
SP-treated
SP,
patients
withdrew from
the study,
oneBoth
due to
an adverse
One D-0042 SP-treated
patients
without differences
between
groups.
treatments
didexperience.
not modify prostate
size,and
residual
volume postexperienced
experiences.
These results
were
confirmed
a second
phase
III study. One
voiding. Two adverse
SP-treated
patients withdrew
from the
study,
one dueinto
an adverse
experience.
One hundred
D-004(100)
patients
withexperienced
mild to moderate
were double-blindly
randomised
to D-004
SP (320
and 2 eligible
SP-treated
patients
adverseBPH
experiences.
These results
were confirmed
in aorsecond
mg/day
capsules
for 24 weeks.
After 2patients
months with
on therapy,
D-004 andBPH
SP lowered
mean IPSS values .
phase IIIboth)
study.
One hundred
(100) eligible
mild to moderate
were double-blindly
This
effect increased
the
study,
IPSSboth)
decreasing
70.3%
with
D- 004After
and 269.0
% with
after 6D-004
months
randomised
to D-004 over
or SP
(320
mg/day
capsules
for 24
weeks.
months
on SP
therapy,
on
groups.
Both over
treatments
decreased
significantly
the with
residual
andtreatment,
SP loweredwithout
mean differences
IPSS values.between
This effect
increased
the study,
IPSS decreasing
70.3%
Dvolume
as after
compared
to baseline.
D-004,
not SP,
reducedbetween
significantly,
butBoth
modestly, the
004 and post-voiding
69.0 % with SP
6 months
on treatment,
without
differences
groups.
prostate
size
and PSA
values versus
baseline.
No significant
between
group to
differences
treatments
decreased
significantly
the residual
volume
post-voiding
as compared
baseline. were
D-004,found.
not
Summarizing
D-004 (320 but
mg/day)
was as
SP
(320
mg/day)
decreasing
LUTS
in men with
SP, reduced significantly,
modestly,
theeffective
prostate as
size
and
PSA
values for
versus
baseline.
No significant
moderate
BPH. Both treatments were well tolerated.
betwe
Key words: D-004, patient, benign prostatic hyperplasia
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-O-021
Phytochemical analysis and wogonin isolation from Scutellaria havanensis Jacq
Marrero Delange, David; Morales Rico, Carmen Luisa; Salas Oliver, Eva; Cuéllar Cuéllar, Armando; González
Canavacciolo, Víctor L.
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
Scutellaria havanensis Jacq. species, known as Escudo de la Habana (Havana skullcaps), is a Cuban
endemic
and it is Jacq.
etnomedically
treatment
scabies,(Havana
and as skullcaps),
diaphoretic is
and
febrifuge.
Scutellariaplant
havanensis
species, used
knownforasthe
Escudo
de laofHabana
a Cuban
endemic
It
is also cultivated
plant and it
foris ornamental
etnomedically
purposes.
used forSeveral
the treatment
pharmacologic
of scabies,
researches
and as diaphoretic
have confirmed
and febrifuge.
that total
extracts
or flavonoids
of Scutellaria
genusSeveral
have pharmacologic
shown antiinflammatory,
antithrombotic,
It is also cultivated
for ornamental
purposes.
researches anxiolityc,
have confirmed
that total
neuroprotective,
anticonvulsivant,
antitumor, antiarthritic,
antiviral,
and antimicrobial
extracts or flavonoids
of Scutellariaantioxidant,
genus haveanticancer,
shown antiinflammatory,
anxiolityc,
antithrombotic,
properties.
Little is
known about the
chemical composition
of this plant.
In this regards,
the and
aim of this work
neuroprotective,
anticonvulsivant,
antioxidant,
anticancer, antitumor,
antiarthritic,
antiviral,
was
to carry out
a phytochemical
study about
of the the
ethereal,
methanolic
and aqueous
extracts
from
aerial the
parts
antimicrobial
properties.
Little is known
chemical
composition
of this plant.
In this
regards,
(leaves
andwork
stems)
havanensis
species. Saponins,
bitter principles,
groups, alkaloids,
aim of this
wasoftoS.carry
out a phytochemical
study ofsugars,
the ethereal,
methanolicamino
and aqueous
extracts
flavonoids,
coumarins,
steroids,
quinones
andSaponins,
resins, insugars,
the extracts
were qualitatively
from aerial parts
(leaves triterpenes
and stems)and
of S.
havanensis
species.
bitter principles,
amino
identified.
The wogonin
flavone
was crystallized
fromsteroids,
the ethereal
extract,
which inwas
structurally
groups, alkaloids,
flavonoids,
coumarins,
triterpenes and
quinones
and resins,
the extracts
characterized
by their
GC-MS
NMR spectra.
The crystallized
identified groups
of compounds
in thiswhich
plant,was
mainly
were qualitatively
identified.
Theand
wogonin
flavone was
from the
ethereal extract,
flavonoids,
be of potential
interest.
structurally could
characterized
by theirpharmacological
GC-MS and NMR
spectra. The identified groups of compounds in this
Key words: scutellaria havanensis, phytochemical, flavonoids, wogonin, lamiaceae, gc-ms
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-O-022 Natural Products from Palauan Traditional Medicine increase Insulin Levels and
Reduce Blood Sugar
Kitalong, Christopher U.; Figueroa, Mario; Kitalong, Ann; Balick, Michael J.; Graz, Bertrand; Yano,
Victor
Palau Republic
[email protected]
On the
the islands
islands ofofPalau,
Palau,traditional
traditionalplant
plantmedicines
medicines
have
been
in the
survival
of small
the small
isolated
have
been
vitalvital
in the
survival
of the
isolated
populations for generations, pre and post-foreign contact. This traditional medicinal knowledge
knowledge has mostly
mostly
been passed
passed on
onthrough
throughoral
oraltradition.
tradition.
Through
ethnobotanical
ethnomedical
surveys,
a Palauan
Through
ethnobotanical
andand
ethnomedical
surveys,
a Palauan
panacea, that most widely used
used for
for the
thetreatment
treatment ofofdiabetes
diabetesmellitus
mellitustype
typeIIIIwas
wasanalyzed
analyzedforforphytochemical
content.
Mangiferin,
a compound
in significant
in this
Palauan
Traditional
with
phytochemical
content.
Mangiferin,found
a compound
found quantities
in significant
quantities
in this
Palauanmedicine
Traditional
diabetes
activity,
was measured
in aqueous
preparations
traditional medicine
and compared
medicine inhibition
with diabetes
inhibition
activity, was
measured
in aqueousofpreparations
of traditional
medicine to
and compared
those
of organictosolvent
those ofextracts.
organic Aqueous
solvent extracts.
extractsAqueous
were most
extracts
effective
were
in isolating
most effective
mangiferin.
in isolating
The insulin
mangiferin. of
stimulation
The
theinsulin
extracted
stimulation
mangiferin
of the
was
extracted
measured
mangiferin
using a ?-cell
was measured
stimulationusing
assay.
a ?-cell
Insulinstimulation
secretion was
assay. Insulin
induced
by the
secretion
extracted
wasmangiferin
induced byinthea extracted
glucose mangiferin
concentration
in a dependent
glucose concentration
manner. Further
dependent
clinical
manner. Further
mechanistic
trialsclinical
were performed
mechanistic
to trials
assess
were
blood
performed
sugar levels
to assess
and insulin
blood quantities
sugar levels
in and
human
insulin
serum with
quantities
and
withoutin traditional
human serum
medicine
with and
preparations
without traditional
during anmedicine
Oral Glucose
preparations
Tolerance
during
Test.anInsulin
Oral Glucose
secretion and
blood
sugarTest.
levelsInsulin
showed
potential
this Palauan
traditional
therapeutic
for treatment of
Tolerance
secretion
andofblood
sugar levels
showedmedicine
potentialasofathis
Palauan traditional
type II Diabetes Mellitus.
Key words: palau, mangiferin, diabetes mellitus type ii
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-O-023
The anti-inflammatory activity of Artemisia afra
Pool, Edmund John; Kisten, Najwa
University of the Western Cape, South Africa
[email protected]
Artemisia afra is a shrub indigenous to South Africa and has been used for medicinal purposes. This study
investigated
the effects
of leaf
extracts
activity using
usingRAW264.7
RAW264.7
study investigated
the effects
of leaf
extractson
on inflammatory/anti-inflammatory activity
macrophages. Curcumin,
Curcumin, aaknown
knownanti-inflammatory
anti-inflammatoryphytochemical
phytochemical
was
also
analysed
concurrently
was
also
analysed
concurrently
to to
compare the relative effectiveness of these two phytoproducts.
phytoproducts. Interleukin
Interleukin 66 and
and nitric
nitric oxide
oxide were
were used
used as
as
biomarkers for inflammation. Artemisia
Artemisia was
was extracted
extracted with
with ethanol.
ethanol. RAW264.7
RAW264.7macrophages
macrophages were
werecultured
cultured
to
confluence
to confluence
in 96 well
in culture
96 well trays.
culture Extracts
trays. Extracts
were tested
were for
tested
anti-inflammatory
for anti-inflammatory
activityactivity
by the by
following
the
following Medium
method:
method: was
Medium
replaced
was replaced
with medium
with medium
containing
containing
various various
concentrations
concentrations
of leave
of leave
extracts
extracts
in the
in the presence
presence
and absence
and absence
of 1 µg/ml
of 1 µg/ml
LPS. LPS.
After After
24 hour
24 incubation
hour incubation
culture
culture
supernatants
supernatants
were were
removed and
removed
tested
for and
nitrictested
oxidefor
production
nitric oxide
(Griess
production
reagent)
(Griess
and interleukin
reagent) and
6 (DAS
interleukin
ELISA).
6 (DAS
The cells
ELISA).
wereThe
tested
cellsfor
were tested
viability
usingfora viability
WST1 assay.
using aThe
WST1
results
assay.
of this
The
study
results
show
of that
this study
Artemisia
showafra
that
is Artemisia
very effective
afra in
is inhibiting
very
effective
the
inflammatory
in inhibiting
activity
the inflammatory
due to LPS exposure
activity due
(IC50
to LPS
at 170
exposure
ug/ml).(IC50
Curcumin,
at 170 ug/ml).
which isCurcumin,
a highly purified
which
component
of turmeric,
has anofIC50
of 3 ug/ml.
presented
NF?ß
and sex on
steroid
is a highly purified
component
turmeric,
has anData
IC50 isofalso
3 ug/ml.
Data on
is also
presented
NF?ßreceptor
and
inhibitors on the anti-inflammatory activity of Artemisia afra.
Key words: anti-inflammatory activity, Artemisia afra
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-O-024
Cuban Medicinal Plants Journal. Present times and Perspectives
Martínez Hormaza, Ioanna ;García Hernández, Ana Ibis
Laboratorio central de Farmacología. Hospital Salvador Allende, Cuba
[email protected]
Introducción: La
LaRevista
RevistaCubana
Cubana
de de
Plantas
Plantas
Medicinales
Medicinales
(RCPM)
(RCPM)
fundada
fundada
en 1996,
en ofrece
1996, a ofrece
los
a los
profesionales del
del campo
campodedela la
salud
salud
y sus
y sus
afines,
afines,
la posibilidad
la posibilidad
de publicar
de publicar
los resultados
los resultados
de sus de sus
investigaciones acerca del
del uso
usode
delas
lasplantas
plantasmedicinales.
medicinales.Expertos
Expertosreconocidos
reconocidosmedian
median
el proceso
el proceso
de de
arbitraje. La
La RCPM
RCPMpretende
pretendecontribuir
contribuir
a laa prevención
la prevención
de enfermedades
de enfermedades
y al mejor
y al manejo
mejor manejo
de los de los
pacientes, y al
al mejoramiento
mejoramiento de
de lalacalidad
calidaddedevida.
vida. Objetivo: Analizar el trabajo de la revista durante los
Objetivo:5 Analizar
el trabajo
la revista
durante
los últimos
5 años. en los últimos 5 años. Con los cuales
últimos
años. Métodos:
se de
revisaron
todos
los números
publicados
de
analizaron
estadísticamente
principales
datos de
autores
más
países y la
Métodos:
se revisaron
todos los los
números
publicados
en la
losrevista,
últimoslos
5 años.
Con
lospublicados,
cuales de analizaron
estadísticamente
producción
científica
los de
principales
la misma.datos
Los datos
de la revista,
fueron analizados
los autores en
más
una
publicados,
base de datos
países
Access.
y la producción
Resultados:
científica
los
cubanos
de la
son
misma.
los autores
Los datos
que más
fueron
publican
analizados
en la en
revista
una base
seguida
de datos
por Colombia
Access.y Brasil. En el año 2013
Resultados:
se
publicaronlos
60cubanos
artículosson
en los
total
autores
siendoque
el año
máscon
publican
mayoren
producción
la revista seguida
científica.por
LaColombia
temática ycon
Brasil.
mayor
En el año
número
de2013
publicaciones
se publicaron
es Farmacología
60 artículos en
y los
total
Master
siendo
enelCiencias
año conson
mayor
los que
producción
más publican
científica.
en laLarevista.
temática con mayor
Conclusiones:
Los resultados
número de
demuestran
publicaciones
la importancia
es Farmacología
de RCPM
y los
y que
Master
se debe
en Ciencias
incrementar
son los
la visibilidad
que más
ennúmero
la revista.
ypublican
elevar el
de citas por artículo. Dedicado a: DrC. Francisco José Morón Rodríguez
Conclusiones: Los resultados demuestran la importancia de RCPM y que se debe incrementar la
visibilidad y elevar el número de citas por artículo.
Key words: Cuban Medicinal Plants Journal
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-O-025
Vegetal biotechnology in heterolog proteins production to prevent and treat diseases
Calva Calva, Graciano
Instituto Politécnico Nacional, México
[email protected]; [email protected]
Plant-based molecular farming using
using transgenic
transgenic plants,
plants, and
and plant
plant cell,
cell,tissue,
tissue,and
andorgan
organcultures
cultureshave
havebeen
been investigated
investigated
as alternative
as alternative
production
production
platforms
platforms
for heterologous
for heterologous
proteins
proteins
produced
produced
in mammal,
in mammal,
microbial,
microbial,
and
insectand
cellinsect
systems.
cell systems.
Now commercial
Now commercial
plant cell
plant
culture
cell culture
processes
processes
includeinclude
tobacco
tobacco
and carrot
and cell
carrot cell suspension
suspension
cultures in
cultures
bioreactors
in bioreactors
with defined
with defined
synthetic
synthetic
medium,
medium,
and and
a number
a number
of of
plantplant-derived
derived pharmaceutical
pharmaceutical
heterologous
heterologous
proteinsproteins
are close
aretoclose
the market.
to the market.
Plant systems
Plant systems
can becan
grouped
be grouped
into three
into
three categories:
categories:
seed-based
seed-based
systems,
systems,
leaf-based
leaf-based
systems,
systems,
and bioreactor-based
and bioreactor-based
systems.
systems.
In this
In this
work,
work,
plant
plant biotechnology
biotechnology
systems
systems
are discussed
are discussed
for the
for the
production
production
of of
therapeutic
therapeutic
proteins.
proteins.Hairy
Hairyroot
rootcultures
cultures of
of Brassica
Brassica
oleracea
oleracea
var.var.
italica
italica
(broccoli)
(broccoli)
areare
used
used
as example
as example
to evaluate
to evaluate
the the
production
production
of the
of human
the human
growt h
growth hormone
hormone
(hGH1) (hGH1)
or somatotropin,
or somatotropin,
insulin,insulin,
and Human
and Human
Papilloma
Papilloma
Virus L1
Virus
protein.
L1 protein.
The major
The major
advantages
advantages
of
transgenicofroots
transgenic
are thatroots
they are genetically
that they are
homogeneous,
genetically homogeneous,
morphologically
morphologically
stable, and thestable,
heterologous
and
the heterologous
is inheritable.
Therefore,
they
are capable
synthesizing proteins
the
protein
productionprotein
trait is production
inheritable. trait
Therefore,
they are
capable of
synthesizing
theofheterologous
heterologous
for
long periods
proteins
of time.
forOne
longofperiods
the most
of important
time. One limitations
of the most
forimportant
the commercial
limitations
exploitation
for the commercial
of hairy rootsexploitation
based
systems
of hairy
for production
roots-based
of secondary
systems formetabolites
production and
of secondary
heterologous
metabolites
proteins and
has heterologous
been the low yields,
proteins has and
productivity
beenthe
thedesign
low yields,
of proper
productivity
bioreactors
and the
that
design
permit
of proper
both growth
bioreactors
of thethat
rootpermit
tissues,
bothnormally
growth
of the rootdistributed
unevenly
tissues, normally
throughunevenly
the bioreactor,
distributed
andthrough
expression
the bioreactor,
of the transgenic
and expression
product.ofInthe
any
transgenic
case, the
product. Inviability
economic
any case,
of the
theproduction
economic process
viability seems
of the production
to be ensured
process
by eliminating
seems to be
lowensured
yields and
by low
eliminating
recovery
by
elite
with high
expression
and by
developing
scalable
processes.
lowselecting
yields and
lowlines
recovery
by selecting
elitelevels,
lines with
high
expression
levels, manufacturing
and by developing
Key words: transgenic protein, agrobacterium rhizogenes, pcambia, gusplus
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-O-026 Isolation and characterization of antiretroviral proteins from Curcuma Longa L.
extracts. Results od three multicentric clinical trials in patients with HIV and Hepatitis
B and C
García Martín, Juan de Jesús; Kaifer, A.; Kaplan, L.; Echegoyen, L.
The Curcuma longa L. is a promissory medicinal plant with anti retroviral properties. A new I.J. protein with
The
longa
is a promissory
medicinal
plant with
anti retroviral
properties. A new
I.J. protein
19KdCurcuma
M.W. and
two L. isomers
is isolated
carry through
Capilar
Zone Electrophoresis,
HPLC,
IRFT and
with
19Kd
M.W.
and
two
isomers
is
isolated
carry
through
Capilar
Zone
Electrophoresis,
HPLC,
Circular
Dichroism
the Convex
Algorithm
test anti
the Secondary
sequentialAstructure
ofIRFT
the ARV
The Curcuma
longaperforming
L. is a promissory
medicinal
planttowith
retroviral properties.
new I.J. protein
and
Dichroism
performing
Convex
Algorithm
toCapilar
test the
Secondary
sequential
structure
ofM.W.
the
protein.
The
IJ protein
purifiedisthe
and
freeze
dried
for further
studies.
Two isomers
or 8
Kd each
with Circular
19Kd
M.W.
and twowas
isomers
isolated
carry
through
Zone
Electrophoresis,
HPLC,
IRFT
ARV
appeared
protein.
seen
as
intrachain
bonded
Diketopyperazins.
A
generic
250
mg
Natural
Drug
Softgels
and Circular Dichroism performing the Convex Algorithm to test the Secondary sequential structure of theis
performed for Clinical Trials in HIV, Hepatitis B, Hepatitis C. volunteers. The performed trials showed the
ARV protein.
reduction
of the
viral
charge
in freeze
HIV, from
by studies.
ml to 0 Two
performing
was
The IJ protein
was
purified
and
dried100,000
for further
isomersthe
or PCR
8 Kd test.
each The
M.W.results
appeared
89.90%
of
VCR by bonded
ml. In patients
with Hepatitis B and C the results were 96.75% VCR respectively. An Ïn
seen
as
intrachain
Diketopyperazins
The
IJ protein
was purified
and freeze
dried
for further
isomers
Kd each
appeared
Vitro¨Action
Mechanism
is Done.
The IJ
protein
showsstudies.
a fusionTwo
inhibition
of or
the8 virus
over M.W.
the the
Hep-2
seenand
as intrachain
bonded Diketopyperazins
liver
HIV Lymphocytes
cells surface in Tissue Culture.
A generic 250 mg Natural Drug Softgels is performed for Clinical Trials in HIV, Hepatitis B, Hepatitis C.
volunteers.
Themg
performed
trials Softgels
showed the
reduction for
of the
viral Trials
chargeininHIV,
HIV,Hepatitis
from 100,000
by ml C.
to 0
A generic 250
Natural Drug
is performed
Clinical
B, Hepatitis
performing
the
PCR
test.
The
results
was
89.90%
of
VCR
by
ml.
In
patients
with
Hepatitis
B
and
C
the
Key
words:
Isolation,
characterization,
antiretroviral
proteins,
Curcuma
Longa
L.
clinical
trials,
patients,
volunteers. The performed trials showed the reduction of the viral charge in HIV, from 100,000 by ml to 0
, Hepatitis
B
results
were 96.75%
VCR respectively.
.HIV
An Ïn Vitro¨Action Mechanism is Done. The IJ protein shows a fusion inhibition of the virus over the the
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-O-027 Potentialities of the use of lipdic extract from Cuban Royal palm fruits in China
population
Hongying, Li
WEGO, Canada
[email protected]
(HPB)
es una
que puede
los hombres
La Hiperplasia
Hiperplasia Prostática
ProstáticaBenigna
Benigna
(HPB)
es patología
una patología
que afectar
puede a afectar
a los hombres
50años
años yycuya
cuyafrecuencia
frecuencia aumenta
aumentacon
conlalaedad.
edad.En
Enchina
chinaseseestima
estimaque
quetengan
tenganesta
fundamentalmente 50
esta afectación
más25demillones
25 millones
de hombres
el 2020.
Esta patología
consiste
un
afectación
más de
de hombres
hastahasta
el 2020.
Esta patología
consiste
en un en
agrandamient
o
agrandamiento
benignoque
de puede
la próstata
queobstrucción
puede causar
obstrucción
y propicia
el desarrollo
síntomas
benigno de la próstata
causar
y propicia
el desarrollo
de síntomas
del de
tracto
bajo
del
tractoentre
bajo los
urinario
entre los que
destacandel
disminución
delorina
volumen
de orinade
y la
la
urinario
que destacan
disminución
volumen de
y la presión
la presión
micción,deretención
micción,
y aumento
la nicturia.
De acuerdo
al grado la
deintensidad
obstrucción,
intensidad
urinaria yretención
aumentourinaria
de la nicturia.
De de
acuerdo
al grado
de obstrucción,
delalos
síntomasde
los
síntomas
variar,
llegando
a afectar
calidad
de hombres
vida de los
que laSepadecen.
puede
variar, puede
llegando
a afectar
la calidad
delavida
de los
quehombres
la padecen.
plantea Se
que su
plantea
queresponde
su desarrollo
responde
a cambios
hormonales
y no
que ocurren
durante el El
desarrollo
a cambios
hormonales
y no
hormonales
quehormonales
ocurren durante
el envejecimiento.
envejecimiento.
El aumento dede
la la
transformación
de en
la testosterona
(T) en dihidrotestosterona
a
aumento de la transformación
testosterona (T)
dihidrotestosterona
(DHT), a través de la(DHT),
enzima
través
de la enzima
5 -reductasa
prostática,
el principal
cambio
hormonal
y el no
principal
5 -reductasa
prostática,
es el principal
cambioes
hormonal
asociado
y el
principal asociado
componente
hormonal
componente
porlael musculatura
aumento del lisa
tonode
delalavejiga
musculatura
lisa de la
vejiga ypor
la los
está dado pornoelhormonal
aumentoestá
del dado
tono de
y la próstata,
regulado
próstata,
regulado (ADR)por los 1..
adrenoreceptores
(ADR)1.. La principal
de inhibidores
la HPB la
adrenoreceptores
La principal terapia
farmacológica
de laterapia
HPB lafarmacológica
constituyen los
constituyen
los inhibidores
delos
la 5
-reductasa prostática,
de ADR
y la terapia
de la 5 -reductasa
prostática,
antagonistas
de ADR -1, los
y laantagonistas
terapia combinada
con-1,
ambos,
todos ellos
combinada
ellos asociados
un númerocomo
importante
de efectos
la
asociados acon
un ambos,
número todos
importante
de efectosa adversos
la disminución
de adversos
la líbido, como
impotencia,
disminución
impotencia,
trastornos ortostática.
de la eyaculación,
la hipotensión
En china,
trastornos dedelala líbido,
eyaculación,
la hipotensión
En china,
con largaortostática.
tradición del
empleo
con
larga tradición
del empleo
etnomédico
de productos
existen como
diferentes
formulaciones
etnomédico
de productos
naturales,
existen
diferentesnaturales,
formulaciones
baishu,
dangsheng,
como
baishu,
dangsheng,
danggui,
ect.,
variables,
éxito deque
su uso
bajo, mientras
danggui,
ect., son
muy variables,
pero
el son
éxitomuy
de su
uso es pero
bajo, el
mientras
en elesmundo
occidental
que
en el
el mundo
mundo
occidental
occidental
el
extracto
extractoha
lipídico
lipídico
de
Sawplametto
plametto
ha
hasido
sidolalaalternativa
alternativa
fitoterapéutica
fitoterapéutica
más
más
el extracto
lipídico
de Saw el
plametto
sido de
la Saw
alternativa
fitoterapéutica
más comúnmente
usada.
comúnmente
se han
handeencontrado
encontrado
reporteslípido
de un
unobtenido
nuevo
nuevo extracto
extracto
lípido
lípido
obtenido
obtenido
de
Recientementeusada.
se hanRecientemente
encontrado reportes
un nuevo extracto
de la palma
real
cubanade
la
palma realregia),
cubana
regia), con
amplia información
avalan su
eficacia en
(Roystonea
con(Roystonea
amplia información
preclínica
que avalan preclínica
su eficaciaque
en modelos
experimentales
modelos
experimentales
y su
la par que
los ensayos
clínicos
muestran
y su seguridad
toxicológica
a seguridad
la par que toxicológica
los ensayosaclínicos
muestran
resultados
prometedores.
Este
resultados
prometedores.
Esteuna
extracto
está
por una
mezcla de ácidos
grasos,
principalmente
extracto está
constituido por
mezcla
deconstituido
ácidos grasos,
principalmente
oleico,
palmitico,
láurico,
oleico,
láurico,
linoleico
mirístico,de
y previene
el desarrollo
de roedores
la HP inducida
con los
T encambios
linoleicopalmitico,
y mirístico,
y previene
el ydesarrollo
la HP inducida
con T en
así como
roedores
así ycomo
los cambios
y urodinámicos
queinducida
caracterizan
a la HP atípica
inducida
por
histológicos
urodinámicos
quehistológicos
caracterizan
a la HP atípica
por fenilefrina,
efectos
que se
fenilefrina,
se relacionan
una inhibición
la 5 -reductasa
y una inhibición
de laspor los
relacionan efectos
con unaque
inhibición
de la 5con
-reductasa
y una de
inhibición
de las respuestas
mediadas
respuestas
mediadas
por los
1. Además
presenta
moderada acción
anti- a la
adrenoreceptores
(ADR)1. adrenoreceptores
Además presenta(ADR)moderada
acción
anti- inflamatoria,
asociada
inflamatoria,
asociada
a la inhibición
dual de las
enzimas
ciclooxigenasa
5-lipooxigenasa
(5inhibición dual
de las enzimas
ciclooxigenasa
(COX)
y 5-lipooxigenasa
(5-(COX)
LOX). yEstudios
en voluntarios
LOX).
enbien
voluntarios
mostró ser
tolerado, que
sin producir
las EA,
mientras
en
sanos Estudios
mostró ser
tolerado,sanos
sin producir
las bien
EA, mientras
en pacientes
sanos
y con que
HP mostró
pacientes
sanos y con Por
HP mostró
efectos
antioxidantes.
Por durante
otra parte
de fue
320tan
mg/día
durante
4y
efectos antioxidantes.
otra parte
a dosis
de 320 mg/día
4 ya 6dosis
meses
efectivo
como
6
tan efectivo
comolos
el STBU
SP (320
mg/día) en
reducir la
losreducción
STBU evaluados
mediante
la reducción
el meses
SP (320fuemg/día)
en reducir
evaluados
mediante
del puntaje
IPSS, produciendo
del
puntaje
produciendo
un descenso
modesto
volumen
prostático.
Unase
variante
de
además
un IPSS,
descenso
modestoademás
del volumen
prostático.
Una del
variante
de este
producto
encuentra
este
producto
sesuplemento
encuentra registrado
suplemento
nutricional
en Cuba
con
el nombre
de Palmex,
registrado
como
nutricionalcomo
en Cuba
con el nombre
de Palmex,
que
dado
la información
que
que
dado la pudiera
información
quecandidato
lo sustenta
pudiera ser
un tomado
candidato
a sercomo
tomado
lo sustenta
ser un
importante
a ser
enimportante
consideración
unaenalternativa
consideración
como una
en el Benigna
manejo de
Prostáticachina.
Benigna en la
natural en el manejo
de laalternativa
Hiperplasianatural
Prostática
en la
la Hiperplasia
población masculina
Key words: lipdic extract, Cuban Royal palm fruit,
China, IPB
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-O-028 In-vitro anthelmintic activity of Mucuna pruriens (DC) and Canarium schweinfurthii
(Engl) on Acaris suum
Habila, James D.; Okoli, B. J.; Ayo, R. G.; Japhet, S. L.; Ndukwe, G. I.
Department of Chemistry, Ahmadu Bello University, Nigeria
[email protected]
Helminth infections are among the most widespread infections in humans, especially
especially in
in the
the tropical
tropical regions
regions
of
the world.
of theItworld.
causes
It causes
enormous
enormous
hazard hazard
to health
to health
and contributes
and contributes
to the to
prevalence
the prevalence
of malnourishment
of
,
malnourishment,
anemia
and pneumonia
anemia among
and pneumonia
others. Mucuna
among pruriens
others. Mucuna
and Canarium
pruriensschweinfurthii
and Canariumused
schweinfurthii
in traditional
used in traditional
medicinal
practicesmedicinal
in Northern
practices
Nigeria,
in Northern
for the treatment
Nigeria, for
of worms
the treatment
infestation
of worms
were investigated.
infestation were
The ininvestigated.
vitro
anthelmintic
The evaluation
in-vitro anthelmintic
of the crude
evaluation
and solvent
of the
partitions
crude and
extracts,
solventonpartitions
Pheretima
extracts,
pothuma
onand
Pheretima
Ascaris
pothuma
suum
(eggs
andand
Ascaris
L2 stage)
suumrespectively.
(eggs and L2Showed
stage) respectively.
that the hexane
Showed
fraction
that the(leaves
hexaneand
fraction
stem bark)
(leaves
of C.
and stem bark)and
schweinfurthii
of C.
Mucuna
schweinfurthii
pruriens and
(leaves)
Mucuna
had pruriens
ovicidal potency
(leaves) of
had
(100.00
ovicidal
±0.33)
potency
at 80mg/ml
of
which was
(100.00 ±0.33)
comparable
to at
the
80mg/ml
positivewhich
control;
was Albendazole(100.00
comparable to the positive
±0.33) control;
at the Albendazole(100.00
same concentration.±0.33)
Whileatthe
the same concentration.
Chloroform
fractions showed
Whileovicidal
the Chloroform
activity offractions
98.20±0.12
showed
and ovicidal
98.20± activity
0.12 80ofmg/ml
98.20±0.12
on theand
two98.20±
plants
0.12 80 mg/ml
respectively.
The
onresults
the twoofplants
determination
respectively.
of larvicidal
The results
potency
of determination
showed that the
of larvicidal
hexane fraction
potency(0.06%)
showedwas
that most
the
the hexane
potent,fraction
followed(0.06%)
by thewas
Chloroform
the mostfractions
potent, followed
(0.1 andby
0.2the
%Chloroform
respectively).
fractions
These(0.1
results
and clearly
0.2 %
respectively).
justified
the uses
These
of these
results
plants
clearly
in the
justified
treatment
the uses
of worms
of these
in ethnoplants medicinal
in the treatment
practices.
of worms in ethno-
Key words: anthelmintic activity, mucuna pruriens, canarium schweinfurthii, pheretima pothuma, a. suum,
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-O-029
Establishment of septic culture of Adenophyllum aurantium
Sánchez Espinosa, Ana Claudia; Cruz, Jorge Alberto; Calderón Oropeza, Mónica Alicia; Ramírez
Hernández, José Eduardo
The role and variety
control.Such
variety of
ofchemical
chemicalcompounds
compounds ininplants
plantsmake
makethem
thempotential
potentialcandidates
candidatesforforpest
pest
is
the case ofisAdenophyllum
aurantium (L.)
Strother,whose
root extract root
containing
acetate
reduced
control.Such
the case of Adenophyllum
aurantium
(L.) Strother,whose
extractethyl
containing
ethyl
the
mycelial
growth
sporulation
of phytopathogenic
fungusFusariumfungusFusarium
solani and Alternaria
acetate
reduced
the and
mycelial
growth and
sporulation of phytopathogenic
solani alternata,
and
Alternaria
without
harming
alternata,
the growth
withoutofharming
the biofertilizer
the growth
Bacillus
of thelicheniformis.
biofertilizer Bacillus
Biotechnological
licheniformis.
techniques
Biotechnological
have been
techniques
used
to obtain
havenatural
been products
used to obtain
that are,
natural
for some
products
reason,
that hard
are, for
to obtain
some reason,
at largehard
scaletothrough
obtain at
chemical
large
scale through
synthesis.
Saidchemical
techniques
synthesis.
are useful
Saidtotechniques
preserve germplasm;
are useful to
additionally,
preserve germplasm;
they help toadditionally,
prevent excessive
they
help to prevent
predation
of wild
excessive
populations
predation
whichofmaintains
wild populations
?to a certain
which maintains
extent? biodiversity
?to a certainand
extent?
genebiodiversity
stability of
and gene stability
species.Due
to theofabove,
species.Due
strategies
to the
to above,
obtain astrategies
large number
to obtain
of individuals
a large number
for its of
use
individuals
in phytochemical,
for its
use in phytochemical,
structural
and metabolic
structural
studiesareimportant.
and metabolic studiesareimportant.
Preliminary resultsPreliminary
show thatresults
we obtained
show that 95%
we of
obtained 95% of
establishment
of establishment
aseptic cultures
of aseptic
from seeds.
cultures
Micro-cutting
from seeds.were
Micro-cutting
sectionedwere
from sectioned
germinated
from
seedlings,
germinated
and
the induction
seedlings,
of multiple
and thesprouts
induction
fromof axillary
multiplebuds
sprouts
wasfrom
achieved
axillary
using
budsMS
wasmedium
achieved
(Murashige
using MSand
Skoog,
supplemented
with
BAPsupplemented
(2.5 milligrams
perBAP
litre)
and
AIA (0.1per
milligrams
medium 1962)
(Murashige
and Skoog,
1962)
with
(2.5
milligrams
litre) andper
AIAlitre).Root
(0.1
forming occurred spontaneously in 90% of the micro-cuttings.
Key words: adenophyllum aurantium, micropropagation, medicinal plant
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-O-030
Round Table: 25 years validation of the medicinal plants use
Duménigo González, Abel; Victoria Amador, María del Carmen; López Barreiro, Marisol; Brito Álvarez,
Gisselle; García Mesa, Milagros; Acosta de La Luz, Lérida; Blanco Hernández, Yisel; Morejón Rodríguez ,
Zulema; García Hernández, Ana Ibis; Martínez Hormaza, Ioanna
Hospital Salvador Allende, Cuba
[email protected]
Introducción:
Introducción: Desde
Desde 1988
1988 el
el Laboratorio
Laboratorio Central
Central de
deFarmacología
Farmacología (LCF)
(LCF)apoyada
apoyadapor
porelelMinisterio
MinisteriodedeSalud
Pública
(MINSAP),
ha
trabajado
en
las
investigaciones
los
usos
de
plantas
medicinales
con
objetivo
Salud Pública (MINSAP), ha trabajado en las investigaciones los usos de plantas medicinaleselcon
el de
incorporarlas
en
el
Sistema
Nacional
de
Salud.
Además
ha
coordinado
el
Programa
Cubano
objetivo de incorporarlas en el Sistema Nacional de Salud. Además ha coordinado el Programa Cubanode
Investigaciones
de de
Plantas
Medicinales
de Investigaciones
Plantas
Medicinales(1988-2004).
(1988-2004).Objetivo: Exponer el trabajo realizado durante 25
años
del Exponer
LCF en el
la trabajo
validación
del uso
tradicional
plantas
se revisaron
Objetivo:
realizado
durante
25 añosdedel
LCF enmedicinales.
la validaciónMétodos:
del uso tradicional
de los
principales
resultados,
trabajos
de
difusión
y
la
docencia
medica
superior,
así
como
los
resultados
plantas medicinales.
publicados
los incluidos
en la Farmacopea
de yTRAMIL
desde
1988superior,
hasta 2014.
Métodos: seyrevisaron
los principales
resultados,Vegetal
trabajosCaribeña
de difusión
la docencia
medica
así
Resultados:
se
expone
el
trabajo
realizado
con
plantas
científicamente
validadas
durante
25
como los resultados publicados y los incluidos en la Farmacopea Vegetal Caribeña de TRAMILaños,
desdeen el
cual
un elevado porcentaje de usos tradicionales respaldados por evaluaciones experimentales
1988 existe
hasta 2014.
preclínicas,
ratifica el
la trabajo
ausencia
casi total
deplantas
toxicidad
en las formas validadas
de uso tradicional.
presenta
Resultados: que
se expone
realizado
con
científicamente
durante 25Seaños,
en el el
trabajo
de
colaboración
con
la
red
TRAMIL
para
la
difusión
del
uso
tradicional,
su
eficacia
y
seguridad
cual existe un elevado porcentaje de usos tradicionales respaldados por evaluaciones experimentales en
Cuba
y el Caribe.
Conclusiones:
Los
datos
la importancia
de lade
validación
del usoSe
tradicional
preclínicas,
que ratifica
la ausencia
casi
totalindican
de toxicidad
en las formas
uso tradicional.
presenta de
plantas,
la
adecuada
divulgación
de
los
resultados
y
la
necesidad
de
establecer
una
metodología
adecuada
el trabajo de colaboración con la red TRAMIL para la difusión del uso tradicional, su eficacia y seguridad
para
la validación.
en Cuba
y el Caribe.
Conclusiones: Los datos indican la importancia de la validación del uso tradicional de plantas, la
adecuada la
Key words: plantas medicinales, farmacopea vegetal caribeña, fitoquímica, farmacología, microbiología,
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-001 Effects of policosanol pre-treatment on blood-brain barrier damage and inflammatory
markers after ischemia-reperfusion brain injury in rats
Pérez Guerra, Yohani; Oyarzábal Year, Ambar; Mas Ferreiro, Rosa María; Molina Cuevas, Vivian;
Jiménez Despaigne, Sonia
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
Blood-brain barrier (BBB) disruption following ischemia-reperfusion (I/R) is associated with poor outcomes
in
stroke patients.
a mixture
of sugarcane
wax alcohols,
hasis been
shownwith
to protect
Blood-brain
barrier Policosanol,
(BBB) disruption
following
ischemia-reperfusion
(I/R)
associated
poor against
cerebral
histological
disturbances
by I/R wax
in gerbils.
Nevertheless,
the effects
of
outcomesfunctional
in stroke and
patients.
Policosanol,
a mixtureinduced
of sugarcane
alcohols,
has been shown
to
policosanol
on experimentally
induced
disruption
have not been
explored.
objective
of this study
protect against
cerebral functional
and BBB
histological
disturbances
induced
by I/R The
in gerbils.
Nevertheless,
was
to investigate
the effects
of policosanol on
BBB disruption
following
induction
of cerebral
the effects
of policosanol
on experimentally
induced
BBB disruption
havethenot
been explored.
TheI/R in rats.
Rats
wereofrandomized
intotoa investigate
negative vehicle
control
five I/R on
groups:
a positive vehicle
three
objective
this study was
the effects
of and
policosanol
BBB disruption
followingcontrol,
the
policosanol
200 I/R
andin400
one randomized
aspirin (150 into
mg/kg).
Treatments
given
1 hgroups:
before
induction of (50,
cerebral
rats.mg/kg),
Rats were
a negative
vehiclewere
control
andorally
five I/R
ischemia
Brain control,
ischemia
was policosanol
induced
by (50,
30
of carotid
arteries,
followed
groups:
a
positivea induction.
positive
vehicle control,
vehicle
three
policosanol
three
(50, 200
andmin
200
400bilateral
and
mg/kg),
400occlusion
one
mg/kg),
aspirin
one
(150
aspirin
mg/kg).
(150 mg/kg).
by
24 h reperfusion.
BBB
permeability
was
evaluated
according
to Evans
bluewas
(EB)induced
dye extravasation.
Treatments
were given
orally
1 h before
ischemia
induction.
Brain
ischemia
by 30 min Also,
myeloperoxidase
activity
in brain
homogenates
was measured.
concentration
MPO values
bilateral occlusion(MPO)
of carotid
arteries,
followed
by 24 h reperfusion.
BBBEB
permeability
was and
evaluated
after
24 h of
significantly
increased
positive controls (MPO)
when compared
according
to reperfusion
Evans blue were
(EB) dye
extravasation.
Also,inmyeloperoxidase
activity in with
brainthe negative
controls.
Oral was
pre-treatment
policosanol (50
- 400
extravasation
homogenates
measured.with
EB concentration
and
MPOmg/kg)
values significantly
after 24 h ofdecreased
reperfusionEB
were
(60.4%
- 71.7%)
as compared
to the
positive
controls.
Aspirin
reduced
significantly
BBB
leakage
by 92.5%.
significantly
increased
in positive
controls
when
compared
with
the negative
controls.
Oral
pre-treatment
Also,
the I/R-induced
increases
MPO activities
were EB
lowered
significantly
with- policosanol
(50 - 400
with policosanol
(50 - 400
mg/kg)ofsignificantly
decreased
extravasation
(60.4%
71.7%) as compared
mg/kg)
(46.3%controls.
- 65.3) and
aspirin
(68.4%).
In conclusion,
oral policosanol
(50 - 400 mg/kg)
to the positive
Aspirin
reduced
significantly
BBB leakage
by 92.5%.pre-treatment
Also, the I/R-induced
protected
damage
cerebral I/R
rats, and attenuated
the increase
MPO, a
increases against
of MPO BBB
activities
were induced
lowered by
significantly
withinpolicosanol
(50 - 400 mg/kg)
(46.3% of- 65.3)
marker
of inflammation,
in the brain oral
tissue.
and aspirin
(68.4%). In conclusion,
policosanol pre-treatment (50 - 400 mg/kg) protected against BBB
Key words: policosanol, blood-brain barrier damage, inflammatory markers, ischemia-reperfusion,
brain injury, rat
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-002 Comparative study of the preventive effects of Policosanol and the omega-3 fatty
acid of fish oil in models of cerebral ischemia
Mena Valdés, Licet ;Molina Cuevas, Vivian; Ravelo Calzado, Yazmin; Noa Puig, Miriam; Valle Clara,
Maikel; Pérez Guerra, Yohani; Oyarzábal Year, Ambar; Mas Ferreiro, Rosa María; Mendoza Hernández ,
Nilda; Jiménez Despaigne, Sonia
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
Stroke is the third cause of death and the first of permanent adult disability. Pre-treatment with policosanol
and
omega-3
fish oil
(FO)ofhas
been
models
of cerebral
ischemia with
in rodents. This
Stroke
is the third
cause
death
andeffective
the firstinofexperimental
permanent adult
disability.
Pre-treatment
study
compared
the effectsfish
of policosanol
oil in the global
cerebral
ischemiaischemia
(GCI) model
policosanol
and omega-3
oil (FO) has and
beenomega-3
effectivefish
in experimental
models
of cerebral
in
induced
by ischemia-reperfusion
(I/R) inofMongolian
Gerbils fish
were
distributed in 7
rodents. This
study compared the effects
policosanolgerbils.
and omega-3
oil randomized
in the global cerebral
groups:
negative
control
group
and 6 with GCI: a positive
(vehicle),
policosanol
(100 and 200
ischemiaa(GCI)
model
induced
by ischemia-reperfusion
(I/R) incontrol
Mongolian
gerbils.
Gerbils were
mg/kg)
and FO
(1.25 and
g/kg g/kg)
and aspirin
mg/kg).
treatments
were administered
by oral
randomized
distributed
in 72.5
groups:
a negative
control(60
group
and All
6 with
GCI: a positive
control (vehicle),
route
during(100
4 weeks.
Policosanol
(100
200and
mg/kg)
andg/kg)
FO (1.25
and 2.5(60
g/kg)
significantly
reduced
policosanol
and 200
mg/kg) and
FOand
(1.25
2.5 g/kg
and aspirin
mg/kg).
All treatments
the
symptoms
score
(63.6during
and 73.3%;
32.7
and 58.8%,
respectively),
the rate
clinical
wereclinical
administered
by oral
route
4 weeks.
Policosanol
(100
and 200 mg/kg)
andofFO
(1.25symptoms
and 2.5
(60
y 60%;
33.4 y 36.4%,
the hyper-locomotion
y 82.5%;
26.7
y 100%,
g/kg)
significantly
reducedrespectively),
the clinical symptoms
score (63.6 (84.4
and 73.3%;
32.7
and
58.8%,respectively),
respectively),the
histological
score symptoms
of neurological
(41.4
y 52.1%;
28.3 y 32.6
respectively) and(84.4
the yplasmatic
the rate of clinical
(60 y damage
60%; 33.4
y 36.4%,
respectively),
the%,
hyper-locomotion
82.5%;
levels
malondialdehyde
y 83.3%; 59.9
y 72.7%,
respectively)
and(41.4
sulphydril
groups
y 100%,
26.7 y of
100%,
respectively),(76.7
the histological
score
of neurological
damage
y 52.1%;
28.3(100
y 32.6
%,
respectively).
In conclusion,
Policosanol
(100 and 200 mg/kg)
FO (1.25
2.5 g/kg)
markedand
and
respectively) and
the plasmatic
levels of malondialdehyde
(76.7 yand
83.3%;
59.9 yand
72.7%,
respectively)
significantly
reduced
GCI in respectively).
Mongolian gerbils,
with a mayor
potency (100
and efficacy
Policosanol.
sulphydril groups
(100the
y 100%,
In conclusion,
Policosanol
and 200ofmg/kg)
and FO
(1.25 and 2.5 g/kg) marked and significantly reduced the GCI in Mongolian gerbils, with a mayor potency
Key words: policosanol, omega-3 fish oil, cerebral ischemia, mongolian gerbils
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-003
Long term stability study of D002 active ingredient
Morales Rico, Carmen Luisa; Sierra Pérez, Roxana de la Caridad; González Canavacciolo, Víctor L.;
Vicente Murillo, Roxana; Rodríguez Leyes, Eduardo Antonio; Marrero Delange, David
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
El D002 es una mezcla de alcoholes grasos
grasos (C24
(C24 aa C34)
C34) extraídos
extraídos yy purificados
purificados de
de la
la cera
cerade
de abejas
abejas Apis
mellifera,
el cual
constituye
el ingrediente
activo
(IA)
BWA®las
lascuales
cuales
Apis mellifera,
el cual
constituye
el ingrediente
activo
(IA)dedelas
lastabletas
tabletas de
de Abexol® yy BWA®
presentan efectos antioxidantes yy gastroprotectores.
gastroprotectores. El
Elpresente
presente estudio
estudioevaluó
evaluó lalaestabilidad
estabilidaddede3 3lotes
lotes
almacenados bajo condiciones de degradación forzada, estabilidad acelerada (40ºC y 75%
75% HR) yy a largo
largo
plazo (3
(3 años)
años)enenlaslaszonas
zonas
climáticas
IV. parámetros
Los parámetros
medidos
en los
todos
los fueron:
casos fueron:
climáticas
II y IIIV.y Los
medidos
en todos
casos
características organolépticas, contenido
contenido (%)
(%)total
totaleeindividual
individualdedealcoholes
alcoholes
mediante
cromatografía
mediante
cromatografía
de de
gases con columna capilar, aparición
aparición de
de productos de
de degradación
degradación así
así como
como las
las pérdidas
pérdidas por
por secado
secado en
en
los casos de
de la
la estabilidad
estabilidad acelerada
acelerada yyaalargo
largoplazo.
plazo.Los
Losresultados
resultadosmuestran
muestranque
quebajo
bajocondiciones
condicionesdede
estrés (hidrólisis ácida y básica, oxidación, termólisis y fotólisis) no se formaron productos de
de degradación
ydegradación
se mantuvo yelseporcentaje
deporcentaje
pureza superior
al 87%.
Por su
los estudios
mantuvo el
de pureza
superior
al parte,
87%. Por
su parte, de
losestabilidad
estudios deacelerada
(12
meses) acelerada
y a largo plazo
demostraron
la plazo
elevada
estabilidad ladeelevada
estos alcoholes.
no se
estabilidad
(12 meses)
y a largo
demostraron
estabilidadAdicionalmente,
de estos alcoholes.
observó,
bajo ninguna
de lasbajo
condiciones
ensayadas,
cambios
de coloración
extraños,
Adicionalmente,
no se observó,
ninguna de
las condiciones
ensayadas,
cambios ni
de olores
coloración
ni
permaneciendo
IA como un polvo
deun
consistencia
cerosa
y color blanco
Las pérdidas
olores extraños, el
permaneciendo
el IA fino
como
polvo fino de
consistencia
cerosa hueso.
y color blanco
hueso. por
secado
fueronporinferiores
al 1% inferiores
lo cual sealcorresponde
las especificaciones
de calidad para
Las pérdidas
secado fueron
1% lo cual secon
corresponde
con las especificaciones
de dicho
producto.
Losdicho
resultados
del presente
estudiodel
confirman
tiempoconfirman
de vida útilundetiempo
3 años,delovida
cualútil
concuerda
calidad para
producto.
Los resultados
presenteunestudio
de 3
con
losloinformados
en estudios
de estabilidad
delprevios
D002 ydesus
formas terminadas.
años,
cual concuerda
con los previos
informados
en estudios
estabilidad
del D002 y sus formas
Key words: d002, alcoholes de la cera de abejas, alcoholes grasos, estabilidad
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-004
Long term stability study of 20 mg Policosanol tablets in blisters
Sierra Pérez, Roxana de la Caridad; González Canavacciolo, Víctor L.; Vicente Murillo, Roxana;
Rodríguez Leyes, Eduardo Antonio; Marrero Delange, David
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
El policosanol constituye una mezcla reproducible
reproducible de
de ocho
ocho alcoholes
alcoholes grasos
grasos primarios
primarios de
de 24
24 aa 34
34átomos
átomos
de carbono
carbonopurificados
purificados
la cañaEsta
desustancia,
azúcar. con
Esta
sustancia, con efectos
de lade
ceralade cera
la cañadede azúcar.
efectos
hipocolesterolemizantes y antiagregante plaquetarios,
plaquetarios, es
es empleada
empleada en
enlalaelaboración
elaboración de
detabletas
tabletascon
con2020
mg de alcoholes, forma farmacéutica terminada utilizada en los
ensayos
clínicos
y
en
la
práctica
de
los
clínicos y en la práctica de rutina.
El
objetivo
fue determinar
la estabilidad
de estas
tabletas
en blísteres
de cloruro
de polivinilo
y aluminio.
rutina.
El objetivo
fue determinar
la estabilidad
de estas
tabletas
en blísteres
de cloruro
de polivinilo
y
Muestras
de tres lotes
de tabletas
se Cuba)
pusieron
cajas de
mantuvieron
en las
aluminio. Muestras
de tres
lotes de(MedSol,
tabletas Cuba)
(MedSol,
seen
pusieron
encartón
cajas ydesecartón
y se
condiciones
climáticadeIVla(30
C, 70 IV
5%
durante relativa)
cinco años.
Los
mantuvieron de
en la
laszona
condiciones
zona2climática
(30de2humedad
C, 70 5 relativa)
% de humedad
durante
parámetros
se mantuvieron
de sus especificaciones
de calidad durante
el estudio:
cinco años. evaluados
Los parámetros
evaluados dentro
se mantuvieron
dentro de sus especificaciones
de todo
calidad
apariencia
(tabletas
verdes
con superficies
peso
promedioenteras),
(inicial peso
10 %),promedio
contenido
total de10los
durante todo
el estudio:
apariencia
(tabletas enteras),
verdes con
superficies
(inicial
seis
alcoholes total
grasos
± 2 mg),
tiempograsos
de desintegración
30 min),
y contenido microbiológico
%), contenido
de (20
los seis
alcoholes
(20 ± 2 mg), (<
tiempo
de desintegración
(< 30 min), y (1000
bacterias/g
y ? 100 hongos/g,
ausencia
de E. coli,
S. aureus,
Pseudomonas,
C. albicans). Los
contenido microbiológico
(? 1000
bacterias/g
y ? 100
hongos/g,
ausencia deSalmonella
E. coli, S. yaureus,
resultados
del Salmonella
presente estudio
sustentan
las tabletas
de policosanol
con dosis
detabletas
20 mg,
Pseudomonas,
y C. albicans).
del presente
estudio sustentan
que las
Los que
resultados
almacenadas
lasdosis
condiciones
de la
zona climática
tiempo
de climática
vida útil de
años.
de policosanolencon
de 20 mg,
almacenadas
en IV,
las presentan
condicionesunde
la zona
IV,cinco
presentan
Key words: policosanol, cera de caña de azúcar, alcoholes grasos, estabilidad, tabletas
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-005
Stability of Abexol® tablets in blisters and high density polyethylene flasks
González Canavacciolo, Víctor L.; Sierra Pérez, Roxana de la Caridad; Morales Rico, Carmen Luisa;
Vicente Murillo, Roxana; Rodríguez Leyes, Eduardo Antonio; Marrero Delange, David
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
Los alcoholes de la
la cera
cera de
de abejas
abejas constituyen
constituyenuna
unamezcla
mezclareproducible
reproducible de
deseis
seisalcoholes
alcoholesgrasos
grasosprimarios
primarios
de
24 a 34
de átomos
24 a 34 átomos
de carbono
de carbono
purificados
purificados
de la cera
de la de
ceraApis
de Apis
mellifera.
mellifera.
EstaEsta
sustancia,
sustancia,
concon
efectos
efectos antioxidantes
y gastroprotectores,
es empleada
la elaboración
de las tabletas
de Abexol®
antioxidantes
y gastroprotectores,
es empleada
para la para
elaboración
de las tabletas
de Abexol®
con dosis
con
dosis de
de alcoholes,
50mg de alcoholes,
forma farmacéutica
utilizada
en los clínicos
ensayosy clínicos
y en lade
de 50mg
forma farmacéutica
terminada terminada
utilizada en
los ensayos
en la práctica
prácticaElde
rutina.fue
El objetivo
fue la
determinar
la de
estabilidad
de las
50 mg dede
alcoholes
rutina.
objetivo
determinar
estabilidad
las tabletas
contabletas
50 mg con
de alcoholes
cera dede
abejas
cera de abejas
(Abexol®)
enfrascos
blísteres
en frascos de
dealta
polietileno
de Muestras
alta densidad.
Muestras
detabletas
tres lotes
(Abexol®)
en blísteres
y en
dey polietileno
densidad.
de tres
lotes de
de
de tabletas
Abexol®
con
dedosis
Abexol®
de 50mg
con dosis
envasadas
de 50mgenenvasadas
blísteres de
en cloruro
blísteresdedepolivinilo
cloruro de
y aluminio
polivinilo yy en
aluminio
frascos
y en
de
frascos de de
polietileno
polietileno
alta densidad
de alta (Rainbow
densidad &
(Rainbow
Nature, &
Sydney,
Nature, Australia)
Sydney, Australia)
se pusieron
se pusieron
en cajas en
de cajas
cartóndey se
cartón y se mantuvieron
mantuvieron
en las condiciones
en las condiciones
de la zona climática
de la zonaIVclimática
(30 2 C,
IV (30
70 52%C,de70humedad
5 % de relativa)
humedaddurant e
relativa)
cinco
años.
durante
En ambos
cinco años.
envases,
En los
ambos
parámetros
envases,evaluados
los parámetros
se mantuvieron
evaluadosdentro
se mantuvieron
de sus especificaciones
dentro de
suscalidad
de
especificaciones
durante todo
de calidad
el estudio:
durante
apariencia
todo el(tabletas
estudio: apariencia
blancas con
(tabletas
superficies
blancas
enteras),
con superficies
peso promedio
enteras),7,5
(inicial
peso
%),promedio
contenido(inicial
total de7,5
los%),
seis
contenido
alcoholes
total
grasos
de los(50
seis
± 3,75
alcoholes
mg), grasos
tiempo (50
de desintegración
± 3,75 mg),
(<
tiempo
15
min),dedureza
desintegración
(> 3 kg/cm2)
(< 15y min),
contenido
dureza
microbiológico
(> 3 kg/cm2) y(?contenido
1000 bacterias/g
microbiológico
y ? 100 (?
hongos/g,
ausencia
1000 bacterias/g
y ? E.
de
100coli,
hongos/g,
S. aureus,
ausencia
Pseudomonas,
de E. coli, S.Salmonella
aureus, Pseudomonas,
y C. albicans).
Salmonella
Los resultados
y C. albicans).
del presente
Los
estudio
resultados que
sustentan
del las
presente
tabletas
estudio
de Abexol®
sustentan
conque
dosis
las de
tabletas
50mg,de
envasadas
Abexol® en
conambos
dosis de
envases,
50mg, envasadas
almacenadas
en las
ambos
condiciones
envases, dealmacenadas
la zona climática
en las
IV,condiciones
presentan un
de tiempo
la zonade
climática
vida útilIV,
de presentan
cinco años.
un tiempo de
Key words: abexol, alcoholes de la cera de abejas, alcoholes grasos, estabilidad, tabletas
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-006 Inhibition of ciclooxygenase and 5-lipooxygenase enzymes by D-002 (beeswax
alcohols)
Pérez Guerra, Yohani; Oyarzábal Year, Ambar; Mas Ferreiro, Rosa María; Molina Cuevas, Vivian;
Jiménez Despaigne, Sonia
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
D-002, a mixture of six higher aliphatic beeswax alcohols, has been shown to produce anti-inflammatory
effects
experimental
modelsaliphatic
and to exhibit
gastroprotective,
not gastrotoxic
effects. The
mechanism of
D-002, in
a mixture
of six higher
beeswax
alcohols, has been
shown to produce
anti-inflammatory
the
anti-inflammatory
D-002,
however,
had not been
yet.effects.
This study
was aimed of
at
effects
in experimentalaction
modelsofand
to exhibit
gastroprotective,
notexplored
gastrotoxic
The mechanism
investigating
the effects
of D-002
on cyclooxygenase
and 5- lipoxygenase
(5-LOX)
activities
in
the anti-inflammatory
action
of D-002,
however, had not(COX)
been explored
yet. This study
was aimed
at
vitro.
The addition
of D-002
to preparations
of the (COX)
cytosolic
of rat blood
polymorphonuclear
investigating
the effects
of D-002
on cyclooxygenase
andfraction
5- lipoxygenase
(5-LOX)
activities in
leukocytes
significantly
(p<0.001)
and dose-dependently
inhibited
thepolymorphonuclear
in vitro activity of 5-LOX
vitro. The addition
of D-002
to preparations
of the cytosolic(r=0.990)
fraction of
rat blood
up
to 81% significantly
at 500?g/mL
concentration.
In contrast, nordihydroguaiaretic
the of
referenc
leukocytes
(p<0.001)
and dose-dependently
(r=0.990) inhibited acid
the in(NDGA),
vitro activity
5-LOXe
substance,
5-LOX
activity by In55%.
Also,nordihydroguaiaretic
D- 002 produced a acid
significant
(p<the
0.001)
and doseup to 81% atinhibited
500?g/mL
concentration.
contrast,
(NDGA),
reference
dependent
inhibition
of COX
activity
in preparations
of rataliver
microsomes
up toand
61%doseat 2500
substance, (r=0.984)
inhibited 5-LOX
activity
by 55%.
Also,
D- 002 produced
significant
(p< 0.001)
?
g/ml, while(r=0.984)
indomethacin
at 0.4
produced
a 90% inhibition.
D-002
bothup
kinetic
parameters
dependent
inhibition
ofmg/ml
COX activity
in preparations
of rat
liver modified
microsomes
to 61%
at 2500 ?
(Vmax
andindomethacin
Km) of 5-LOX
andmg/ml
COXproduced
activities athrough
uncompetitive
mode ofboth
inhibition.
This study
g/ml, while
at 0.4
90% inhibition.
D-002 modified
kinetic parameters
demonstrates
that
D-002 inhibits
both
COX and
5-LOX
activities, with
a highest
affinity This
for 5-LOX,
(Vmax and Km)
of 5-LOX
and COX
activities
through
uncompetitive
mode
of inhibition.
study so that
the
dual inhibition
both inhibits
enzymeboth
activities
may 5-LOX
explainactivities,
the observed
D-002
demonstrates
that of
D-002
COX and
with aanti-inflammatory
highest affinity foreffects
5-LOX,of so
that
as
the lackofofbotheffects
D- 002-related
gastrotoxicity.
thewell
dualasinhibition
of D-002
Key words:
ciclooxygenase and 5-lipooxygenase, enzymes, D-002, beeswax, alcohols
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-007 Effects of D-002 (beeswax alcohols), grape seed extract and their combined therapy
on oxidative markers in rat plasma and liver
Oyarzábal Year, Ambar
Molina Cuevas, Vivian; Mas Ferreiro, Rosa María; Jiménez Despaigne, Sonia; Curveco, Dayisell Lázara
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
D-002, a mixture of high molecular weight alcohols from beeswax (Appis mellifera), and grape seed extract
(Vitis
L), of
rich
in flavonoids,
presents
antioxidant
no previous
study
had
compared
D-002,vinifera,
a mixture
high
molecular weight
alcohols
from effects,
beeswaxbut(Appis
mellifera),
and
grape
seed such
effects.
The aim
of thisL),study
was
to investigate
the effects
of D-002,
grape
extract and
their
no previous
study
hadcombined
extract (Vitis
vinifera,
rich in
flavonoids,
presents
antioxidant
effects,
butseed
therapy
on such
rat plasma
and
liver
markers.
experiments
conducted.
rats
compared
effects.
The
aimoxidative
of this study
was Two
to investigate
the were
effects
of D-002, First
grapeexperiment:
seed extract
were
distributed
intotherapy
seven groups:
a vehicle
control,
three D-002-treated,
grape seed
extract-treated
and their
combined
on rat plasma
and
liver oxidative
markers. Twothree
experiments
were
conducted.
(25,
and 250 mg/kg/day,
respectively).
Secondgroups:
experiment:
rats control,
were randomized
into four groups:
into seven
a vehicle
three D-002-treated,
three one
First100
experiment:
rats were distributed
vehicle
control
and three treated
withand
D-002,
seed respectively).
extract or theirSecond
combined
therapy (25
grape seed
extract-treated
(25, 100
250 grape
mg/kg/day,
experiment:
ratsmg/kg/day).
were
Treatments
werefour
given
orallyone
for vehicle
30 days.
In the
first
experiment:
D- D-002,
002 andgrape
grape
seed
extract
(25randomized into
groups:
control
and
three
treated with
seed
extract
or their
250mg/kg/day)
plasma Treatments
malondialdehyde,
totalorally
hydroperoxides,
groups andD-liver
combined therapyreduced
(25 mg/kg/day).
were given
for 30 days. Incarbonyl
the first experiment:
malondialdehyde
formation
with similar efficacy,
but unchanged
blood superoxide
dismutase.
Second
002 and grape seed
extract (25-250mg/kg/day)
reduced
plasma malondialdehyde,
total
hydroperoxides,
experiment:
each
monotherapy
produced
significant
and efficacy,
comparable
reductions
carbonyl groups
and liver
malondialdehyde
formation
with similar
but unchanged
bloodof plasma
malondialdehyde,
carbonyl
groups
and livereach
malondialdehyde
The combined
therapy lowered
superoxide dismutase.
Second
experiment:
monotherapy formation.
produced significant
and comparable
plasma
was more
effectively
each
monotherapy, formation.
and reduced
liver
reductionsoxidative
of plasmavariables
malondialdehyde,
carbonyl
groups than
and liver
malondialdehyde
The
malondialdehyde
thanplasma
grape oxidative
seed extract,
not than
D-002.effectively
In conclusions
D-002
and grape and
seed
combined therapy more
lowered
variables
was more
than each
monotherapy,
extract
mg/kg/day) significantly
and similarly
reduced
plasma
oxidative
variables
and and
liver
reduced (25-250
liver malondialdehyde
more than grape
seed extract,
not than
D-002.
In conclusions
D-002
malondialdehyde
without modifying
blood
superoxide
dismutase.
combined
therapy
grape seed extractformation,
(25-250 mg/kg/day)
significantly
and similarly
reduced
plasma The
oxidative
variables
and
displayed
benefits overformation,
each monotherapy
regarding
to plasma
oxidative
variables,
liver malondialdehyde
without modifying
blood
superoxide
dismutase.
The whereas
combinedregarding
therapy to
liver
malondialdehyde
more
effective than
grape seed
extract,
not than
D-002. whereas regarding to
displayed
benefits overwas
each
monotherapy
regarding
to plasma
oxidative
variables
Key words: d-002, grape seed extract, lipid peroxidation, protein oxidation
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-008 Protective effect of D-002, a mixture of beeswax alcohols, against indomethacininduced gastric ulcers and mechanism of action
Pérez Guerra, Yohani; Oyarzábal Year, Ambar; Mas Ferreiro, Rosa María; Molina Cuevas, Vivian;
Jiménez Despaigne, Sonia
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
D-002, a mixture of higher aliphatic beeswax alcohols, produces gastroprotective and antioxidant effects.
To
investigate
theofgastroprotective
of D-002
against
indomethacin-induced
oxidative variables
D-002,
a mixture
higher aliphaticeffect
beeswax
alcohols,
produces
gastroprotective ulcers,
and antioxidant
effects.
and
myeloperoxidase
(MPO) activity
in the
gastric
mucosa
were examined. Rats
wereoxidative
randomized into
To investigate
the gastroprotective
effect
of rat
D-002
against
indomethacin-induced
ulcers,
six
groups:
negative vehicle (MPO)
control activity
and five
indomethacin
(50 mg/kg)
groups,
a
variables
anda myeloperoxidase
in the
rat gastric mucosa
weretreated
examined.
Ratscomprising
were
positive
control,
three
groups
treated vehicle
orally with
D-002
(5, indomethacin
25 and 100 mg/kg)
andtreated
one group
with
randomized
into six
groups:
a negative
control
and five
(50 mg/kg)
groups,
omeprazole
mg/kgcontrol,
intraperitoneally
(ip).
The contents
malondialdehyde
(MDA),
carbonyl
comprising a 20
positive
three groups
treated
orally withofD-002
(5, 25 and 100
mg/kg)protein
and one
group
groups
(PCG), hydroxyl
generation
glutathione peroxidase
(GSH- PX),
with omeprazole
20 mg/kgradical
intraperitoneally
(ip).and
Thecatalase
contents (CAT),
of malondialdehyde
(MDA), protein
superoxide
dismutase
and
MPOgeneration
enzyme activities
in the
rat gastric
mucosa
were assessed.
carbonyl groups
(PCG), (SOD)
hydroxyl
radical
and catalase
(CAT),
glutathione
peroxidase
(GSHIndomethacin
increased
the(SOD)
content
MDAenzyme
and PCG,
the generation
of *OH mucosa
radical and
enzyme
PX), superoxide
dismutase
andofMPO
activities
in the rat gastric
wereMPO
assessed.
activity,
while itincreased
decreased
CAT, GSH-PX
andPCG,
SOD the
activities
as compared
to the and
negative
Indomethacin
thethe
content
of MDA and
generation
of *OH radical
MPO controls.
enzyme D002
(5-100
mg/kg)
significantly
and GSH-PX
dose-dependently
ulceration
to 75D%.
activity,
while
it decreased
the CAT,
and SOD reduced
activities indomethacin-induced
as compared to the negative
controls.
Also,
D-002 mg/kg)
decreased
the content
MDA and PCG, the
generation
of hydroxyl radicals
and MPO
activity
002 (5-100
significantly
and of
dose-dependently
reduced
indomethacin-induced
ulceration
to 75
%.
as
compared
to the positive
controls.
dosethe
of generation
D-002 (100ofmg/kg)
increased
GSHAlso,
D-002 decreased
the content
of The
MDAhighest
and PCG,
hydroxyl
radicals significantly
and MPO activity
PX
and SOD activities,
whilecontrols.
all dosesThe
used
increased
activities.
20 mg/kg,
the referenc
as compared
to the positive
highest
doseCAT
of D-002
(100 Omeprazole
mg/kg) increased
significantly
GSH-e
drug,
reduced
significantly
theallulcers
%),increased
MDA andCAT
PCG,
the generation
of hydroxyl
radicals
PX and
SOD activities,
while
doses(93
used
activities.
Omeprazole
20 mg/kg,
the and MPO
activity,
increased
CAT, GSH-PX
and SOD
activities.
D-002
treatment
produced
gastroprotect
ive
referenceand
drug,
reducedthe
significantly
the ulcers
(93 %),
MDA and
PCG,
the generation
of hydroxyl
radicals
effects
against
indomethacin-induced
gastric
ulceration,
whichactivities.
can be related
to the reduction
of hydroxyl
and MPO
activity,
and increased the CAT,
GSH-PX
and SOD
D-002 treatment
produced
radical
generation,
lipidagainst
peroxidation,
protein oxidation
and ulceration,
MPO activity,
increase
gastroprotective
effects
indomethacin-induced
gastric
whichand
cantobethe
related
to theof the
antioxidant
activities in the rat gastric mucosa.
reduction o enzymes
and
Key words: Protective effect, D-002, beeswax alcohols, indomethacin- induced, gastric ulcers,
mechanism of action
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-009
Effects of D-002 on models of acute inflammation
Carbajal Quintana, Daisy; Molina Cuevas, Vivian; Mas Ferreiro, Rosa María; Ravelo Calzado, Yazmin;
Arruzazabala Valmaña, Lourdes
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
Oral treatment with D-002, a mixture of 6 high molecular weight aliphatic primary alcohols purified from
beeswax,
has been
shown atomixture
produceof anti-inflammatory
effects aliphatic
in the carrageenan-induced
pleurisy
Oral treatment
with D-002,
6 high molecular weight
primary alcohols purified
fromand
in
the cotton
models, but
its effects on other
models
acute inflammation have
not been
beeswax,
haspellet
beengranuloma
shown to produce
anti-inflammatory
effects
in theofcarrageenan-induced
pleurisy
and
investigated.
This study
was aimed
to but
investigate
theonanti-inflammatory
effectsinflammation
of single oralhave
doses
in the cotton pellet
granuloma
models,
its effects
other models of acute
notof D002
dextran-, histaminerat paw
oedema models, effects
and onofthe
xylenebeenon
investigated.
This studyand
wasserotonin-induced
aimed to investigate
the anti-inflammatory
single
oral induced
doses
ear
oedema
in mice. histamineOral pre-treatment
with D-002 (200,
400oedema
and 800
mg/kg)
inhibited
of D-002
on dextran-,
and serotonin-induced
rat paw
models,
andsignificantly
on the xylenedextran-induced
paw in
oedema
(p< 0.001)
up to 71.4%.
D-002 (200,
(800 mg/kg),
or 400
mg/kg, inhibited
400 andnot
800200
mg/kg)
significantly
induced ear oedema
mice. Oral
pre-treatment
with D-002
significantly
histamine - and
serotonin-induced
hinduppaw
oedemas
in rats
57 (p <not
0.01)
36% (p <
inhibited dextran-induced
paw
oedema (p< 0.001)
to 71.4%.
D-002
(800bymg/kg),
200and
or 400
0.001),
respectively,
while diphenhydramine
(60 mg/kg) and serotonin
mg/kg)inproduced
mg/kg, inhibited
significantly
histamine - and serotonin-induced
hind paw (10
oedemas
rats by 57significant
(p < 0.01)
inhibitions
(p <respectively,
0.05) and 79%
(p diphenhydramine
< 0.01), respectively.
Also, D-002
(200 and(10
400mg/kg)
mg/kg),
not 50
and 36% (pof<60%
0.001),
while
(60 mg/kg)
and serotonin
produced
mg/kg,
significantly
dose-dependently
0.951,
0.05)respectively.
reduced by 50
%a
xyleneinduced
oedema
significant
inhibitionsand
of 60%
(p < 0.05) and(r=
79%
(p < p0.01),
Also,
D-002
(200
and 400
formation
mice
ear. significantly
Thus, this study
reveals that oral treatment
single
dosesby
of 50
D-002
exhibited
mg/kg), notin50
mg/kg,
and dose-dependently
p 0.05)
reduced
% a xylene(r= 0.951, with
significant
anti-inflammatory
in experimental
models
of acute
inflammation.
induced oedema
formation inactivity
mice ear.
Thus, this study
reveals
that oral
treatment with single doses of
Key words: D-002, acute inflammation
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-010 Effects of D-002 on aspirin induced ulcers and neutrophil infiltration on the gastric
mucosa
Valle Clara, Maikel; Molina Cuevas, Vivian; Mas Ferreiro, Rosa María; Noa Puig, Miriam; Mendoza Castaño,
Sarahí; Oyarzábal Year, Ambar; Mendoza Hernández, Nilda
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
The
gastric mucosa
mucosaisissusceptible
susceptibletoto
effects
of aggressive
factors,
which
are counterbalanced
by
The gastric
thethe
effects
of aggressive
factors,
which
are counterbalanced
by
mucosal
defensive
factors.
Acid
peptic
diseases
result
from
the
imbalance
between
these
aggressive
and
mucosal defensive factors. Acid peptic diseases result from the imbalance between these aggressive and
defensive
factors. Aspirin-induced
Aspirin-inducedulcer
ulceris ais model
a model
of NSAIDs-induced
damage
in neutrophil
which neutrophil
defensive factors.
of NSAIDs-induced
damage
in which
infiltration
infiltration play
play a
a key
key role.
role. D-002,
D-002, aa mixture
mixture of
of 66 higher
higher aliphatic
aliphatic alcohols,
alcohols, has
has been
been shown
shown gastroprotect ive
and
anti-inflammatory
effects. This workeffects.
investigates
the protective
effect
D-002 against
aspirin-induc
ed
gastroprotective
and anti-inflammatory
This work
investigates
the of
protective
effect of
D-002
ulcers
associated neutrophil
infiltration
in the
gastric mucosa.
were
randomized
sixwere
groups of
againstand
aspirin-induced
ulcers and
associated
neutrophil
infiltrationRats
in the
gastric
mucosa.into
Rats
8
rats each. A
negative
vehicle
and A
five
aspirin vehicle
(300 mg/kg)-treated
groups:
a positive
control, orally
randomized
into
six groups
of 8control,
rats each.
negative
control, and five
aspirin
(300 mg/kg)-treated
treated
the vehicle,
D-002
(25,
and 100
mg/kg,
respectively)
with 10 mg/kg
groups: with
a positive
control,three
orallywith
treated
with
the50
vehicle,
three
with D-002
(25, 50 and
and other
100 mg/kg,
Omeprazole.
Five other
(5) hours
after
induced
damage the
Thedamage
stomachs
removed
respectively) and
with 10
mg/kg
Omeprazole.
Fiverats
(5) were
hourssacrificed.
after induced
thewere
rats were
and
opened,
lesions were
examined
macroscopically
andlesions
microscopically.
indexes and
sacrificed.
Theand
stomachs
removed
and opened, and
examined Ulcer
macroscopically
andneutrophil
infiltration
per ulcer
areas
wereand
measured.
positive, per
noneulcer
negative,
controls
exhibitedAllaspirin-induc
ed
microscopically.
Ulcer
indexes
neutrophilAllinfiltration
areas were
measured.
positive,
ulcers.
Oral treatment
D-002aspirin-induced
(25-100 mg/kg)
dose-Oral
dependently
and D-002
significantly
reduced
none negative,
controls with
exhibited
ulcers.
treatment with
(25-100
mg/kg)aspirindoseinduced
gastric
(37 toreduced
75%), the
mean numbergastric
of microscopic
(40 the
to 72%)
neutrophil
dependently
andlesions
significantly
aspirin-induced
lesions (37ulcers
to 75%),
mean and
number
of
infiltration
(41.7
to 83.1%)
in theand
rat gastric
mucosa.
In turn,
omeprazole
significantly
the
microscopic
ulcers
(40 to 72%)
neutrophil
infiltration
(41.7
to 83.1%)10
in mg/kg
the rat reduced
gastric mucosa.
In turn,
ulcer
indexes,
mean
microscopic
ulcer number
neutrophil
infiltration
per areaulcer
of microscope
omeprazole
10the
mg/kg
reduced
significantly
the ulcerand
indexes,
the mean
microscopic
number andfield,
respectively.
Oral treatment
D-002 (25- field,
100 mg/kg)
effectively
against
aspirin-induced
ulcers
neutrophil infiltration
per areawith
of microscope
respectively.
Oralprotects
treatment
with D-002
(25- 100 mg/kg)
and
in
the
gastric
mucosa
induced
by
aspirin
ulceration.
effecthil infiltration in the
Key words: D-002, aspirin induced ulcer, neutrophil infiltration, gastric mucosa
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-011 Effects of D-002 and Grape Seed Extract on monoiodine-acetate induced
osteoarthritis in rats
Mendoza Castaño, Sarahí; Noa Puig, Miriam; Mas Ferreiro, Rosa María; Valle Clara, Maikel; Mendoza
Hernández, Nilda
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]; [email protected]
Osteoarthritis (OA), the most common age-related degenerative joint disease in adults, is characterized by
byprogressive
a
a progressive
cartilage
cartilage
loss
loss
which
which
produces
producesdebilitating
debilitatingchronic
chronicpain
painininaffecting
affectingindividuals
individuals and
andtends
tends to
to worsen
worsen
over
overtime
timeasascartilage
cartilagewears
wearsaway.
away.Non-pharmacologic
Non-pharmacologic interventions
interventions are
are the cornerstone of
of OA
OA
management. Nevertheless, optimal treatment should
should combine
combine non-pharmacological
non-pharmacological and
and pharmacological
pharmacological
modalities.
Numerous
modalities.
studies
Numerous
have demonstrated
studies havethat
demonstrated
oxidative stress
that oxidative
has beenstress
implicated
has been
in OA which
implicated
support
theinevaluation
OA whichofsupport
antioxidants
the evaluation
for management
of antioxidants
of OA. D-002
for management
is a mixtureof
of OA.
six high
D-002
molecular
is a mixture
weight
of six highalcohols
aliphatic
molecular
purified
weightfrom
aliphatic
beeswax
alcohols
that purified
has been
from
shown
beeswax
to bethat
effective
has been
in experimental
shown to be effective
models of
in experimental
acute
and chronic
models
inflammation
of acute and chronic
in experimental
inflammation
models
andofinOA.
experimental
The aim ofmodels
this study
of OA.
wasThe
to compare
aim of
this study
effects
of was
D-002
to and
compare
Grapeeffects
seed of
extract
D-002(GSE)
and Grape
in mono
seed
iodoacetate
extract (GSE)
induce
in mono
OA iniodoacetate
rats. Male wistar
inducerats
OA indistributed
were
rats. Male into
wistar
6 groups:
rats were
one
distributed
negative into
control
6 groups:
and fiveone
groups
negative
with control
MIA induce
and five
OA:groups
a positive
withcontrol
MIA
induce and
group
OA: four
a positive
groupscontrol
treated
group
withand
D-002
four (200
groups
andtreated
400 mg/kg)
with D-002
or GSE
(200(200
and and
400 400
mg/kg)
mg/kg).
or GSE
OA(200
was
and 400 mg/kg).
induced
by MIA. OA
Substances
was induced
wereby administered
MIA. Substances
during
were
10 administered
days. Cartilage
during
changes
10 days.
were
Cartilage
measured by
changes
using
histological
were measured
and Mankin
by usingmodified
histological
score.
and D-002
Mankin(200
modified
and score.
400 mg/kg)
D-002 (200
and and
GSE400
(400
mg/kg)
mg/kg)
and GSE (400
significantly
prevented
mg/kg) significantly
the increaseprevented
on total histological
the increase
score
on total
induced
histological
by the injection
score induced
of MIA by
intothe
the knee
injection
joint
of the
of MIA
animals.
into the
Effects
knee of
joint
D-002
of thewere
animals.
significantly
Effects of
higher
D-002than
wereGSE
significantly
ones in higher
the reduction
than GSE
of total
histological
score. D-002
(200
and 400 score.
mg/kg)D-002
significantly
reduce
histological
parameters
of the score
ones in the reduction
of total
histological
(200 and
400 mg/kg)
significantly
reduce
histological
except
cellular
parameters
abnormalities
of theand
score
osteoclasts
except cellular
presences.
abnormalities
On its side,
andGSE
osteoclasts
was no effective
presences.
on On
extension
its side,to
GSE was cellular
damage,
no effective
abnormalities,
on extension
bone
to loss
damage,
and panus
cellularformation
abnormalities,
and osteoclast
bone losspresence.
and panus
D-002
formation
was more
and
effective to GSE in preventing cartilage damage in MIA- induced OA model in rats.
Key words: osteoarthritis, d-002, mono iodoacetate, rats
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-012 Effects of D-002, a mixture of high molecular weight beeswax alcohols, on patients
with non- alcoholic fatty liver disease (NAFLD)
Illnait Ferrer, José; Rodríguez, Iván; Mendoza Castaño, Sarahí; González, Yolanda; Mas Ferreiro, Rosa
María; Miranda, Migdalia; Fernández Travieso, Julio César; Mesa, Meilis; Fernández Dorta, Lilia de la C.;
Carbajal D., Daisy; Gámez Menéndez, Rafael
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
BACKGROUND. Non-alcoholic fatty liver disease (NAFLD), characterized by excessive liver fat
accumulation
without
habit of alcohol
consumption,
is intimately
related tobyinsulin
resistance
BACKGROUND.
Non-alcoholic
fatty liver
disease (NAFLD),
characterized
excessive
liver fatand ranges
from
a benign without
course to
liveroffibrosis
cirrhosis. NAFLD
management
mainly
involves
dietary
modification
accumulation
habit
alcoholand
consumption,
is intimately
related to
insulin
resistance
and
ranges
and
loss.
Although
no fully
successful
pharmacological
intervention mainly
is available,
alternative
fromweight
a benign
course
to liver
fibrosis
and cirrhosis.
NAFLD management
involves
dietary therapies
to
treat NAFLD
promisingnoresults.
D-002, apharmacological
mixture of beeswax
alcohols
with antioxidant
modification
and have
weightshown
loss. Although
fully successful
intervention
is available,
effects,
hastherapies
been shown
hepatoprotective
TheD-002,
effectsaofmixture
D-002 of
onbeeswax
NALFD, however,
alternative
to treat
NAFLD have effects
shown experimentally.
promising results.
alcohols
had
not been investigated.
investigate
the efficacyeffects
and safety
of D-002 on
NALFD.
with antioxidant
effects, has AIM:
beenTo
shown
hepatoprotective
experimentally.
Thepatients
effects with
of D-002
on
METHODS:
This placebo-controlled
study included 50 patients with NAFLD randomized to placebo (n =
NALFD, however,
had not been investigated.
25)
50 mg the
(n =efficacy
25) tablets
twice aofday
for 24
endpoint was to obtain a significant
AIM:orToD-002
investigate
and safety
D-002
on weeks.
patientsPrimary
with NALFD.
METHODS:
reduction
of This
ultrasonography-detected
placebo-controlled study
liverincluded
fat infiltration
50 patients
as compared
with NAFLD
to placebo.
randomized
Secondary
to placebo
endpoints
(n =
were
obtain50decreases
of tablets
the Human
(HOMA)
index,was
insulin
levels,a serum
liver
25) ortoD-002
mg (n = 25)
twiceOmeostatic
a day for 24Assesment
weeks. Primary
endpoint
to obtain
significant
enzymes,
increase
of plasma total antioxidant
status (TAS)
and improvement
clinical symptoms
reduction of
ultrasonography-detected
liver fat infiltration
as compared
to placebo. of
Secondary
endpoints as
compared
to placebo.
RESULTS:
At randomization,
all indicators(HOMA)
were comparable
in both
groups.
were to obtain
decreases
of the Human
Omeostatic Assesment
index, insulin
levels,
serumSeven
liver
enzymes,D-002(28.0%)
increase
patients
of plasma
and none
total antioxidant
placebo exhibited
status (TAS)
a normal
andliver
improvement
echopattern
of clinical
ultrasonography
symptoms(p<0.01)
as
at
study completion.
compared
to placebo. Also, D-002 reduced significantly (p<0.01 as compared to baseline and placebo)
HOMA
indexAtand
insulin levels,allincreased
unaffectedinthe
remaining
parameters.
TheD-002frequency of
RESULTS:
randomization,
indicatorsTAS
wereand
comparable
both
groups. Seven
(28.0%)
D002 patients
(12/25,
48.0%)
referring
symptom
higher (p<0.001)
than in
patients
and none
placebo
exhibited
a normal
liverimprovement
echopattern was
ultrasonography
(p<0.01)
at placebo
study (1/25,
4.0%).
Treatment
was safe
and well
tolerated.(p<0.01
Six patients,
three ofto
each
group,and
withdrew
from
the study,
completion.
Also, D-002
reduced
significantly
as compared
baseline
placebo)
HOMA
only
because
of adverse
(100 mg/day)
was effective
indextwo
and(placebo)
insulin levels,
increased
TAS experiences.
and unaffectedCONCLUSION:
the remaining D-002
parameters.
The frequency
of D- to
improve
ultrasonographic
insulin resistance
(HOMA
index, than
insulin
levels), (1/25,
plasma
002 patients
(12/25, 48.0%)findings,
referringindicators
symptom of
improvement
was higher
(p<0.001)
in placebo
TAS
and
clinical evolution
thiswell
population
of Six
patients
with three
NAFLD.
Further
studies,
however,
4.0%).
Treatment
was safeonand
tolerated.
patients,
of each
group,
withdrew
from are
the needed
study,
only
to
confirm
two (placebo)
these results.
because of adverse experiences.
CONCLUSION: D-002 (100 mg/day) was effective to improve ultrasonographic findings, indicators of
insulin resistation of
Key words: D-002, beeswax alcohols, patients non- alcoholic fatty liver disease, NAFLD
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-013 Effects of D-002 (beeswax alcohols) on gastrointestinal symptoms and oxidative
markers in middle-aged and older patients
Fernández Dorta, Lilia de la C. ; Rodríguez, Iván; Molina Cuevas, Vivian; Mendoza Castaño, Sarahí; Mas
Ferreiro, Rosa María; Oyarzábal Year, Ambar; Pérez Guerra, Yohani; Mesa, Meilis; Fernández Travieso,
Julio César; Gámez Menéndez, Rafael; Jiménez Despaigne, Sonia; Ruiz, Dalmer; Cruz, Yadira
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
This study investigated the persistence and coexistence of the effects of D-002, a mixture of higher aliphatic
beeswax
with antioxidant
and gastroprotective
symptoms
andaplasma
This studyalcohols
investigated
the persistence
and coexistenceeffects,
of the on
effects
of D-002,
mixtureoxidative
of highermarkers
in
subjects
with gastrointestinal
complaints. and
Sixty
subjects were effects,
double-blinded
randomised
to D-002 (50
aliphatic
beeswax
alcohols with antioxidant
gastroprotective
on symptoms
and plasma
mg)
or placebo
twicewith
dailygastrointestinal
for 24 weeks.complaints.
Dose was Sixty
titrated
to three
tablets
daily if symptoms,
oxidative
markerstablets
in subjects
subjects
were
double-blinded
assessed
the Gastrointestinal
Symptom
Rating
(GSRS),
did Dose
not improve
after to6 three
weeks. No
randomisedwith
to D-002
(50 mg) or placebo
tablets
twice Scale
daily for
24 weeks.
was titrated
significant changes occurred in placebo. D-002 persistently reduced (p < 0.001) GSRS overall score and
tablets
if symptoms,
the Gastrointestinal
did were
not less
several daily
sub-scores
versusassessed
placebo. with
Dose-titration
(1/30) andSymptom
antacids Rating
intake Scale
(7/30) (GSRS),
with D-002
improve
after
6
weeks.
No
significant
changes
occurred
in
placebo.
D-002
persistently
reduced
(p
< (p <
frequent (p < 0.001) than with placebo (29/30 and 28/30, respectively). D-=02 persistently decreased
0.001) GSRS
score and(pseveral
sub-scores
versus placebo. and
Dose-titration
antacids
0.00001)
total overall
hydroperoxides,
< 0.001)
plasma malondialdehyde
sulphydril (1/30)
groups,and
while
increased
intake
(7/30)
with D-002
were less frequent
(p < 0.001) than
with (100
placebo
(29/30administered
and 28/30, respectively).
(p < 0.01)
plasma
total antioxidant
status. Summarizing,
D-002
mg/day)
for 24 weeks
D-=02 persistently
displayed
persistentdecreased
antioxidant
(p <
and
0.00001)
gastroprotective
total hydroperoxides,
effects in subjects
(p < 0.001)
with plasma
gastrointestinal
malondialdehyde
complaints.
OBJECTIVES:
In light of
these
issues, this
persistence
and coexistence
ofD-002
the effects
and sulphydril groups,
while
increased
(p <study
0.01)investigated
plasma totalthe
antioxidant
status.
Summarizing,
of
D-002,
given
for 6 months,
symptoms
andpersistent
plasma oxidative
markers
of middle-agedeffects
and older
(100
mg/day)
administered
for on
24 the
weeks
displayed
antioxidant
and gastroprotective
in
subjects
with
habitual
gastrointestinal
complaints.
CONCLUSIONS
This
study
demonstrates
that oral
subjects with gastrointestinal complaints.
treatment with D-002 (100 mg/day) for 24 weeks ameliorated symptoms and improved plasma oxidative
markers in middle-aged and older subjects with habitual gastrointestinal complaints. These results support
OBJECTIVES: In light of these issues, this study investigated the persistence and coexistence of the
the coexistence and persistence of symptom relief and antioxidant effects of D-002 and that its antioxidant
effects
of D-002,
given for
6 months,
on the
symptoms
and plasma
markers of middle-aged and
effect could
contribute,
at least
partially,
to its
gastroprotective
effectoxidative
in such population.
older subjects with habitual gastrointestinal complaints.
Key words: D-00, beeswax alcohols,gastrointestinal symptom, oxidative marker, middle-aged
CONCLUSIONS
older patient
This study demonstrates that oral treatment with D-002 (100 mg/day) for 24 weeks ameliorated
symptoms and improved plasma oxidative markers in middle-aged and older subjects with habitual
gastrointestinal complaints. These results support the coexistence and persistence of symptom relief and
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-014
Three years of long term stability of 5 mg Prevenox tablets in blisters
Sierra Pérez, Roxana de la Caridad; González Canavacciolo, Víctor L.; Vicente Murillo, Roxana;
Rodríguez Leyes, Eduardo Antonio; Marrero Delange, David
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
El prevenox constituye
constituyeuna
una mezcla
mezclareproducible
reproducible de
deácidos
ácidosgrasos
grasosdede2424a 36
a 36
átomos
átomos
dede
carbono
carbono
purificados
purificados
de
la cera de
delalacaña
cera de
de azúcar.
la caña Esta
de azúcar.
sustancia,
Estacon
sustancia,
efectos con
antioxidantes,
efectos antioxidantes,
es empleadaesen
empleada
la elaboración
en la
elaboración
de tabletas
5 mg
de ácidos,
forma
terminada
utilizada
enclínicos
los ensayos
en la
de
tabletas con
5 mg de con
ácidos,
forma
terminada
utilizada
en los
ensayos
y en laclínicos
prácticay de
rutina.
práctica
de fue
rutina.
El objetivo
fue determinar
la estabilidad
de blísteres
estas tabletas
en blísteres
de cloruro
de
El objetivo
determinar
la estabilidad
de estas
tabletas en
de cloruro
de polivinilo
y aluminio.
polivinilo yde
aluminio.
Muestras
de tres
lotes de
tabletas
(MedSol,enCuba)
en mantuvieron
cajas de cartón
y
Muestras
tres lotes
de tabletas
(MedSol,
Cuba)
se pusieron
cajas se
depusieron
cartón y se
en las
se mantuvieron
las condiciones
la zona
climática
2 C, 70 relativa)
5 % de humedad
relativa)
condiciones
de en
la zona
climática IVde(30
2 C,
70 5 IV
% (30
de humedad
durante tres
años. Los
durante tres evaluados
años. Los se
parámetros
evaluados
se sus
mantuvieron
dentro de
especificaciones
parámetros
mantuvieron
dentro de
especificaciones
desus
calidad
durante todode
el calidad
estudio:
durante todo
apariencia
(tabletas
el estudio:
verdes
apariencia
con superficies
(tabletas enteras),
verdes con
peso
superficies
promedioenteras),
(inicial peso
10 %),promedio
contenido
(inicial
total de10los
%), contenido
ácidos
grasos total
(5 ±de0,5
losmg),
ácidos
tiempo
grasos
de (5desintegración
± 0,5 mg), tiempo
(< 30
de min),
desintegración
y contenido
(< 30
microbiológico
min), y contenido
(? 1000
microbiológico
bacterias/g
y ? (?
1001000
hongos/g,
bacterias/g
ausencia
y ? 100
de E.
hongos/g,
coli, S. aureus,
ausencia
Pseudomonas,
de E. coli, S.Salmonella
aureus, Pseudomonas,
y C. albicans). Los
Salmonella del
resultados
estudio
Los resultados
sustentandel
quepresente
las tabletas
de sustentan
prevenox con
de 5mg,
y C.presente
albicans).
estudio
que dosis
las tabletas
dealmacenadas
prevenox
conlas
en
dosis
condiciones
de 5mg, almacenadas
de la zona climática
en las condiciones
IV, presentandeunlatiempo
zona climática
de vida útil
IV,de
presentan
tres años.
un tiempo de vida
Key words: prevenox, cera de caña de azúcar, ácidos grasos, estabilidad, tabletas
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-015 Effects of D-003 in the vertebrae and lipid profile in a model of osteoporosis induced
by glucocorticoid and ovariectomy in rabbits
Valle Clara, Maikel; Noa Puig, Miriam; Mendoza Castaño, Sarahí; Pérez Guerra, Yohani; Oyarzábal Year,
Ambar; Nodal Flores, Carlos Ramón; Mendoza Hernández, Nilda; Jiménez Despaigne, Sonia
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
Osteoporosis is a chronic degenerative systemic disease characterized by microarchitectural disrepair of
bone
tissue accompanied
the progressive
reduction
of bone
mass. D-003
is a mixture of verydisrepair
long- chain
Osteoporosis
is a chronic by
degenerative
systemic
disease
characterized
by microarchitectural
of
aliphatic
acids
purified
from
sugarcane
which
has
shown
cholesterol-lowering
effects
in
experimental
bone tissue accompanied by the progressive reduction of bone mass. D-003 is a mixture of very long- and
clinical
studies.acids
Previous
studies
rats havewhich
shownhas
that
D-003
prevent bone loss effects
and decreased
bone
chain aliphatic
purified
from in
sugarcane
shown
cholesterol-lowering
in
resorption
in ovariectomized
rats. Previous
This studystudies
was undertaken
investigate
the effects
of D-003
a model
experimental
and clinical studies.
in rats havetoshown
that D-003
prevent
bone in
loss
and
of
osteoporosis
by in
ovariectomy
and glucocorticoids
in female
rabbits. the
Female
New
decreased
boneinduced
resorption
ovariectomized
rats. This studyadministration
was undertaken
to investigate
effects
of
Zealand
white
rabbits
skeletally
matured
were
used
in
4
groups.
A
sham
group
of
false
operated
rats
D-003 in a model of osteoporosis induced by ovariectomy and glucocorticoids administration in female and
three
ovariectomized
treated
withmatured
prednisolone
animals;
a positive
control
rabbits.groups
FemaleofNew
Zealand white and
rabbits
skeletally
were used
in 4 groups.
A sham
group group
of
administered
and twoofgroups
treated with
with D-003
(50 and
200 mg /
false operatedwith
ratsthe
andvehicle
three groups
ovariectomized
andD-003.
treatedTreatment
with prednisolone
animals;
a positive
kg)
significantly
prevented with
decreased
trabecular
numbertreated
and thickness
while
decreased
trabecular
control
group administered
the vehicle
and two groups
with D-003.
Treatment
with D-003
(50
separation,
of osteoclast
and osteoclast
increased
thethickness
induced damage.
In line with
and 200 mg number
/ kg) significantly
prevented
decreasedsurface
trabecular
numberbyand
while decreased
these
results,
D-003 number
significantly
preventedand
theosteoclast
increase surface
in Totalincreased
cholesterol
andinduced
TG at damage.
both doses
trabecular
separation,
of osteoclast
by the
In
improving
the
lipid
profile
of
animals
treated
with
D-003,
allowing
for
the
first
time
linking
lipid
lowering
line with these results, D-003 significantly prevented the increase in Total cholesterol and TG at botheffect antiresorptive
effect.
doses improving
the lipid profile of animals treated with D-003, allowing for the first time linking lipid
Key words: d-003, glucocorticoids, osteoporosis, rabbit
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-016 Effect of D-003 and soy standardized extract rich in daidzein on osteoporosis induced
in ovariectomized rats
Noa Puig, Miriam; Mendoza Castaño, Sarahí; Valle Clara, Maikel; Mendoza Hernández, Nilda; Mas
Ferreiro, Rosa María
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
D-003 is a mixture of higher fatty acids purified from sugarcane wax with antiosteoporotic effects in
ovariectomized (ovx) rats. Soy standardized extract rich in daidzein (SSE-D) have shown bone-protect ive
effects. This study compared the effect of D-003 and SSE-D on bones of ovx rats. Rats were randomized
into 6 groups: one false-operated and five ovx groups: a positive control treated orally with the vehicle, two
with D-003 (50 and 200 mg/kg) and two with SSE-D (15 and 30 mg/kg) for 3 months. Ovx decreased
trabecular volume, number and thickness of trabeculae in the fifth vertebrae and femoral neck, and
increased trabecular separation, osteclast number and surface versus the false-operated group. D-003 and
SSE-D prevented all changes induced by ovx, but the effects of D-003 were significantly greater than those
of SSE-D. Concluding, D-003 (50 and 200 mg/kg) and SSE-D (15 and 30 mg/kg) prevented osteoporotic
changes in ovx rats, but D-003 was more effective than the SSE-D preparation assayed.
Key words: D-003, soy standardized extract, daidzein, osteoporosis, ovariectomized rat
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-017 Effects of D-003 on quality of life in postmenopausal women at moderate to high risk
of osteoporosis.
Fernández Travieso, Julio César; Ceballos, Alfredo; Illnait Ferrer, José; Mendoza Castaño, Sarahí;
Fernández Dorta, Lilia de la C.; Mas Ferreiro, Rosa María; Gámez Menéndez, Rafael; Mesa, Meilis; Cruz,
Yadira
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
Osteoporosis, a disease characterized by reduction of bone mineral density (BMD) and alteration of bone
microarchitecture
that increases
bone fragility
and fracture
affects
the quality
of lifealteration
of the sufferers
Osteoporosis, a disease
characterized
by reduction
of bone risk,
mineral
density
(BMD) and
of bone.
Pharmacological
osteoporosis
of drugs
that of
reduce
resorption
microarchitecture management
that increases of
bone
fragility andincludes
fracture the
risk,use
affects
the quality
life ofbone
the sufferers.
and/or
increase bone
synthesis.
is a mixture
of high
molecular
weight
aliphaticbone
acids
purified from
Pharmacological
management
ofD-003
osteoporosis
includes
the use
of drugs
that reduce
resorption
sugar
waxbone
withsynthesis.
antiresorptive
demonstrated
in experimental
and clinical
studies.from
This
and/or cane
increase
D-003 effects
is a mixture
of high molecular
weight aliphatic
acids purified
randomized,
double-blinded,
placebo-controlled
study investigated
the effects
of D-003studies.
on the This
quality of life
sugar cane wax
with antiresorptive
effects demonstrated
in experimental
and clinical
of
postmenopausal
women at placebo-controlled
moderate to high risk
of OP.
Forty postmenopausal
were
randomized,
double-blinded,
study
investigated
the effects of women
D-003 on
the randomized
quality of
to
placebo or D-003
(10atmg/d)
for three
months.
on Qualeffo
total
score and
lifereceive
of postmenopausal
women
moderate
to high
risk ofSignificant
OP. Forty reductions
postmenopausal
women
were
serum
low-density
lipoprotein-cholesterol
(LDL-C)
levels
versus
placebo
groupreductions
were considered
as the
randomized
to receive
placebo or D-003 (10
mg/d) for
three
months.
Significant
on Qualeffo
total score
primary
andand
secondary
serum low-density
efficacy variables,
lipoprotein-cholesterol
respectively. Evaluation
of safety
tolerability
(LDL-C) levels
versusand
placebo
groupincluded
were the
considered
analyses
of as
thethe
effects
primary
on physical
and secondary
and blood
efficacy
indicators,
variables,
and the
respectively.
adverse events
(AE) reports,
well. Data
Evaluation
of safetyasand
tolerability
were
analyzed
included
as per
thethe
analyses
Intention-to-Treat
of the effects
method.
on physical
Both groups
and blood
displayed
indicators,
similarand
baseline
the adverse
characteristics.
events
(AE) reports,
After
completeasthree
well. months
Data were
on therapy,
analyzedD-003
as persignificantly
the Intention-to-Treat
decreased method.
Qualeffo Both
total groups
scores displayed
(p<0.0001 vs
similar baseline
baseline,
p<0.05characteristics.
vs placebo), asAfter
well as
complete
LDL-C values
three months
(17.1 %).
on The
therapy,
treatment
D-003was
significantly
safe and decreased
well tolerated.
Qualeffo
Only
one total
patient
scores
(D-003-treated)
(p<0.0001 vs
withdrew
baseline,
from
p<0.05
the study,
vs placebo),
and it was
as due
welltoasprotocol
LDL-C violation.
values (17.1
Four%).
patients,
The
all
from placebo
group,
reported
someOnly
AE.one
In conclusion,
D- 003 (10withdrew
mg/d) administered
during
treatment
was safe
and well
tolerated.
patient (D-003-treated)
from the study,
and three
it
months
quality
of life
in postmenopausal
moderate
to high
risk
of In
OP
and produced
was dueimproved
to protocol
violation.
Four
patients, all from women
placebo at
group,
reported
some
AE.
conclusion,
Dadditional
benefit
on serum LDL-C
003 (10 mg/d)
administered
during values.
three months improved quality of life in postmenopausal women at
Key words: D-003, postmenopausal women, osteoporosis
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-018 Effects of D-003, a mixture of sugarcane wax acids, on bone marrow lesions,
subchondral bone and cartilage damage in monoiodoacetate-induced osteoarthritis
Noa Puig, Miriam
Mendoza Castaño, Sarahí; Mas Ferreiro, Rosa María; Valle Clara, Maikel; Mendoza Hernández, Nilda
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
Osteoarthritis (OA) has been linked with bone marrow edema (BME) pattern, identified by magnetic
resonance
imaging
and been
histopathology,
a mix of(BME)
different
pathologies,
so by
that
BME term has
Osteoarthritis
(OA) has
linked withwhich
bone involves
marrow edema
pattern,
identified
magnetic
been
progressively
replaced
by that ofwhich
bone involves
marrow alesions
(BML). BML
is the first
signBME
of OA
resonance
imaging and
histopathology,
mix of different
pathologies,
so that
termafter
experimental
ligament damage,
cartilage
erosion
and(BML).
degeneration
in experimentally-induc
has been progressively
replaced precedes
by that of bone
marrow
lesions
BML is the
first sign of OA after ed
OA,
strongly correlate
knee precedes
pain incidence
anderosion
progressing
in humans, and
the reduction of BML size
experimental
ligament with
damage,
cartilage
and degeneration
in experimentally-induced
is
accompanied
by pain
improvement.
Some studies
have revealed
positive and
findings
with bisphophonat
OA,
strongly correlate
with
knee pain incidence
and progressing
in humans,
the reduction
of BML es
and
impact on the
progression
of cartilage
Oral revealed
administration
of findings
D-003, awith
mixture of high
size their
is accompanied
by pain
improvement.
Some defects.
studies have
positive
molecular
weightand
sugar
wax
acids,
has beenof shown
produceOral
bone
antiresorptive
effectsa in
bisphophonates
their cane
impact
on the
progression
cartilagetodefects.
administration
of D-003,
experimental
clinical studies.
This study
investigated
thebeen
effects
of D-003
on BML,
subchondral
bone
mixture of highand
molecular
weight sugar
cane wax
acids, has
shown
to produce
bone
antiresorptive
and
cartilage
by sodiumand
monoiodoacetate
OA in rats.the
Animals
distributed
into 5 groups:
effects
in experimental
clinical studies.(MIA)-induced
This study investigated
effectswere
of D-003
on BML,
a
negative control
injected
with MIA:
one positive control
and three with
(100, 200were
and 400
subchondral
bone and four
cartilage
by sodium
monoiodoacetate
(MIA)-induced
OA inD-003
rats. Animals
mg/kg).
Theinto
damage
wasa induced
a single
of MIA
theone
synovial
of and
the knee,
after
distributed
5 groups:
negative by
control
three with
control
and injection
four injected
withinto
MIA:
positivecavity
which
gastricThe
gavage
for 10
Kneebyjoint
were injection
removedofand
for light
D-003 rats
(100,were
200 treated
and 400by
mg/kg).
damage
wasdays.
induced
a single
MIAprocessed
into the synovial
microscopy.
Slidesafter
werewhich
stained
and eosin
and for
toluidine
blue
to stain
proteoglycan
cavity of the knee,
ratswith
werehematoxylin
treated by gastric
gavage
10 days.
Knee
joint were
removed for
analyze
the cartilage.
Mankin score
was were
usedstained
to assess
depth andand
extent
of and
cartilage
damage.
and processed
for lightAmicroscopy.
Slides
withthe
hematoxylin
eosin
toluidine
blue toNo
negative,
but all for
positive
control
animals,A exhibited
BMLs,
D-003
decreased
stain proteoglycan
analyze
the cartilage.
Mankin score
was meanwhile
used to assess
the treatment
depth and extent
of
significantly
the frequency
of animals
with BMLs.
Negative
controls
did BMLs,
not show
tibial plateau
cartilage damage.
No negative,
but all positive
control
animals,
exhibited
meanwhile
D-003 extent of
damage,
loss or
presence the
of osteoclasts,
thesewith
variables
significantly
increased
treatment bone
decreased
significantly
frequency of all
animals
BMLs. were
Negative
controls did
not showin the
positive
control
group.
Oral D-003
ameliorated
the damage
to the subchondral
lowering
tibial plateau
extent
of damage,
bone
loss or presence
of osteoclasts,
all these bone,
variables
were the extent of
tibial
damage,
bone loss,
and
the presence
of osteclasts
in the
knee joint,
compared
significantly
increased
in the
positive
control group.
Oral D-003
ameliorated
theas
damage
to thewith positive
controls.
Oralbone,
treatment
withtheD-003
effective
for reducing
subchondral
and cartilage
subchondral
lowering
extentwas
of tibial
damage,
bone loss,BMLs,
and the
presence ofbone
osteclasts
in the
damage,
OApositive
in the rat
knee, Oral
which
suggests
it may
components of
knee joint,inasMIA-induced
compared with
controls.
treatment
withthat
D-003
wasbenefit
effectivemore
for reducing
BMests that itjoint.
osteoarthitic
Key words: D-003, sugarcane wax acids, bone marrow lesions, bone and cartilage damage,
osteoarthritis
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-019 Stability of D-004 active ingredient in amber glass and high density polyethylene
flasks
Rodríguez Leyes, Eduardo Antonio; González Canavacciolo, Víctor L.; Sierra Pérez, Roxana de la
Caridad; Vicente Murillo, Roxana; Morales Rico, Carmen Luisa; Marrero Delange, David
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
farmacéutico activo
activo obtenido
obtenido de
de los
los frutos
frutos de
de la
lapalma
palma real
real cubana
cubana (Roystonea
El D-004 es un ingrediente farmacéutico
(Roystonea
regia), compuesto
fundamentalmente
por una
ácidos
grasos
libres
8 y 18 de
regia),
compuesto
fundamentalmente
por una mezcla
de mezcla
ácidos de
grasos
libres
entre
8 y entre
18 átomos
átomos decon
carbono,
carbono,
potencialidades
con potencialidades
en el tratamiento
en el tratamiento
de la hiperplasia
de la hiperplasia
prostáticaprostática
benigna. benigna.
Para determinar
Para
la
determinar
vida
útil del la
D-004
vida útil
en frascos
del D-004
de en
vidrio
frascos
ámbar
de yvidrio
de polietileno
ámbar y de polietileno
alta densidad,
de alta
en las
densidad,
zonas climáticas
en las
IV
yzonas
II, muestras
climáticas
de IV
tres
y II,
lotes
muestras
pilotos de
se tres
colocaron
lotes pilotos
a 30 ºC/70
se colocaron
% HR y a2530ºC/
ºC/70
60 %
% HR,
HR se
y 25determinaron
ºC/ 60 % HR,las
se determinaron organolépticas
características
las características
y organolépticas
los contenidosy los
decontenidos
material deinsaponificable,
material insaponificable,
ácidos grasos
ácidos y
grasos y microbiológicos
microbiológicos
desde el tiempo
desde el
inicial
tiempo
hasta
inicial
los 51
hasta
meses,
los 51
y se
meses,
calcularon
y se calcularon
los tiemposlos
de tiempos
vida útil mediante
de vida
útil
mediante
análisis En
estadísticos.
En vidrio
ámbar,
en ambas
zonas
el D-004
sus
análisis
estadísticos.
vidrio ámbar,
en ambas
zonas
el D-004
mantuvo
sus mantuvo
especificaciones
de calidad
especificaciones
de calidad
por más de del
24 meses.
La de
disminución
del contenido
por
más de 24 meses.
La disminución
contenido
ácidos, ocurrida
primerodeenácidos,
la zonaocurrida
IV, permitió
primero en los
la zona
IV, permitió
nuevos
tiempos
útil para en
ambas
zonas.
En el D-004
establecer
nuevos
tiempos establecer
de vida útillos
para
ambas
zonas.deEnvida
polietileno,
ambas
zonas,
polietileno,
ambas
zonas, el
D-004 cumplió
parámetros
evaluados
durante los primeros
30 de
cumplió
conenlos
parámetros
evaluados
durantecon
loslosprimeros
30 meses,
posteriormente
el contenido
meses,disminuyó
posteriormente
contenido
de ácidos
al límite establecido
ácidos
hastaelresultar
inferior
al límitedisminuyó
establecido
hasta
en resultar
varias deinferior
las muestras.
Se demostróenque
varias
de las muestras.
Se demostró
que
el ingrediente
activo D004,
obtenido
a escala
pilotoun
y envasado
el
ingrediente
activo D004,
obtenido a
escala
piloto y envasado
en vidrio
ámbar,
presenta
tiempo de
en
ámbar, presenta
un tiempo
útil en
superior
a losclimáticas
24 meses IV
(33y yII,48
meses en las zonas
vidavidrio
útil superior
a los 24 meses
(33 yde
48vida
meses
las zonas
respectivamente),
mientras
climáticas
al
ser envasado
IV y II,en
respectivamente),
frascos de polietileno
mientras
de alta
al ser
densidad
envasado
presenta
en frascos
tiempos
de polietileno
de vida útilde
dealta
30 ydensidad
33 meses
en las zonas climáticas IV y II, respectivamente.
Key words: d-004, estabilidad, vidrio ámbar, polietileno de alta densidad, roystonea regia
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-020
Preliminary stability of soft gelatin capsules with D-004 active ingredient
Rodríguez Leyes, Eduardo Antonio; González Canavacciolo, Víctor L.; Sierra Pérez, Roxana de la
Caridad; Vicente Murillo, Roxana; Morales Rico, Carmen Luisa; Marrero Delange, David
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
El desarrollo de
de cápsulas
cápsulas blandas
blandasde
deD004,
D004,ingrediente
ingredienteactivo
activocon
con
potencialidades
potencialidades
en en
la prevención
la prevención
y y
tratamiento de
delalahiperplasia
hiperplasiaprostática
prostática
benigna,
benigna,
conllevó
conllevó
al estudio
al estudio
de estabilidad
de estabilidad
de esta de
forma
esta forma
loteslotes
pilotopiloto
de cápsulas
con 320con
mg 320
de ácidos
totales
(LIPA
farmacéutica. Se
Seemplearon
emplearontrestres
de cápsulas
mg de
ácidos
totales (LIPA
PHARMACEUTICALS, Australia)
ámbarámbar
de tereftalato
de polietileno
(40
Australia)envasadas
envasadasen frascos
en frascos
de tereftalato
de polietileno
(40
cápsulas/frasco). El estudio de estabilidad acelerada se realizó a 40 ºC
ºC yy 75 %
% HR
HR yy los
los estudios
estudios aa largo
largo
%%
HR)
y IV
(30(30
ºC ºC
y 70
% %
plazo se desarrollaron
desarrollaron en
en condiciones
condiciones de
delas
laszonas
zonasclimáticas
climáticasII II(25
(25ºCºCy 60
y 60
HR)
y IV
y 70
HR). En el
el tiempo
tiempoinicial
inicialy yenencada
cada
muestreo
muestreo
se se
determinaron
determinaron
las las
Características
Características
organolépticas,
organolépticas,
que que
coincidieron con las iniciales durante
durante los
los seis
seis primeros
primeros meses
meses del
del estudio
estudio acelerado
acelerado yyen
entodos
todoslos
losdemás
demás muestreos
muestreos
de los estudios
de los estudios
a largoaplazo;
largo plazo;
la Variación
la Variación
del peso,
del peso,
que seque
mantuvo
se mantuvo
en 90-en110
90-%110
del%peso
del
peso promedio
promedio
inicial,inicial,
el tiempo
el tiempo
de Desintegración,
de Desintegración,
que fue
que 30
fue min
30 ymin
el Contenido
y el Contenido
microbiológico
microbiológico
(una (una
vez al
vez alque
año),
año),
seque
mantuvo
se mantuvo
estable.estable.
También
También
se determinó
se determinó
el contenido
el contenido
de ácidos
de grasos
ácidos por
grasos
Cromatografía
por
de
Cromatografía
Gases,
el cual se
de mantuvo
Gases, elencual
320se
mgmantuvo
± 32 mgen
(10%)
320 por
mg cápsula
± 32 mg y(10%)
durante
portodo
cápsula
el estudio
y durante
en condiciones
todo el
estudio en condiciones
aceleradas
y en ambas aceleradas
zonas climáticas.
y en ambas
Según
zonas
los resultados
climáticas.del
Según
estudio
los resultados
de estabilidad
del estudio
acelerada,
de se
estabilidad
prevé
que estas
acelerada,
cápsulas
se prevé
pudieran
que resistir
estas cápsulas
hasta 6 meses
pudieran
en resistir
condiciones
hasta drásticas
6 meses yenalcanzar
condiciones
un tiempo
drásticas
de
vida útil
y alcanzar
de 3 años
un tiempo
en las de
condiciones
vida útil dede
3 años
la zona
en las
climática
condiciones
IV, losderesultados
la zona climática
obtenidos
IV, los
hasta el
momento
largo plazo
avalan
un tiempo
de vida
útil avalan
de al menos
2 años
en las
ambas
resultados aobtenidos
hasta
el momento
a largo
plazo
un tiempo
de vida
útilcondiciones
de al menos de
2 años
zonas climáticas.
Key words: d-004, estabilidad, cápsulas blandas, tereftalato de polietileno, roystonea regia
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-021
Determination of degradation products of D004 Active Ingredient
Vicente Murillo, Roxana; Rodríguez Leyes, Eduardo Antonio; González Canavacciolo, Víctor L.; Sierra
Pérez, Roxana de la Caridad; Morales Rico, Carmen Luisa; Marrero Delange, David
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
La determinación de
de los
los productos
productos de
dedegradación
degradacióneneningredientes
ingredientesactivos
activos(IA)
(IA)
y formas
y formas
terminadas
terminadas
es es
esencial como parte
parte de
de su
suconocimiento
conocimientoquímico-farmacéutico
químico-farmacéuticoy yununaspecto
aspectoexigido
exigido
porpor
loslos
organismos
organismos
anterior,
se implementaron
métodos
analíticos
para identificar
y
reguladores. Teniendo
Teniendo en
encuenta
cuentalo lo
anterior,
se implementaron
métodos
analíticos
para identificar
y
cuantificar los
D004.
A muestras
sometidas
a oxidación
(peróxido
de de
los productos
productos de
dedegradación
degradaciónenenel el
D004.
A muestras
sometidas
a oxidación
(peróxido
(80 (80
°C y°C
185y °C),
les determinaron
las características
hidrógeno), fotólisis
fotólisis (luz
(luzUV)
UV)y termólisis
y termólisis
185se°C),
se les determinaron
las características
índice
de ácido
tiobarbitúrico
(IATB),(IATB),
porcentaje
de dienos
organolépticas, índice
índice dedeperóxido
peróxido(IP),
(IP),
índice
de ácido
tiobarbitúrico
porcentaje
de dienos
conjugados (%DC) y compuestos volátiles (CVol), y sus resultados se compararon con el de una
una muestra
muestra
sometidas
a 110
°C solo
se les
los dos
de D004 sin
sin degradar;
degradar; mientras
mientrasque
quea amuestras
muestras
sometidas
a 110
°C solo
se determinaron
les determinaron
los dos
últimos parámetros. Se
mayor
degradación
de de
los los
AG AG
Se determinó
determinó que
quelalaoxidación
oxidaciónfue
fuelalaque
queprovocó
provocóla la
mayor
degradación
insaturados, así como los mayores IP, mientras que la termólisis fue la
la que
que conllevó aa mayores
mayores contenidos
contenidos
de
DC, IATBdey DC,
CVol.
IATB
En yelCVol.
caso En
de el
la caso
termólisis
de la atermólisis
110 °C se
a 110
pudo°Cobservar
se pudo que
observar
el % que
DC aumentó
el % DC hasta
aumentó
las
7 h y hasta
disminuyó
las 7 ah ypartir
disminuyó
de estea tiempo,
partir dese
este
determinó
tiempo, se
pordeterminó
primera vez
primera
presencia
vez la
depresencia
2-hexenal,
de2por la
heptenal,
2-heptanona,2-heptanona,
2-hepteno, tolueno,
octano,
1-pentanol
y pentilfurano
en el D004
2-hexenal,2-hexanona,
2-heptenal, 2-hexanona,
2-hepteno,
tolueno,
octano, 1-pentanol
y pentilfurano
degradado.
Key words: d004, productos de degradación, ácidos grasos, estabilidad
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-022 One year oral Toxicity of D-004, a lipid extract from Roystonea regia fruits, in
Sprague Dawley rats
Gutiérrez Martínez, Ariadne; Gámez Menéndez, Rafael; Noa Puig, Miriam; Mas Ferreiro, Rosa María;
Arencibia Arrebola, Daniel Francisco; Pardo, B.; Valle Clara, Maikel; Oyarzábal Year, Ambar; Curveco,
Dayisell Lázara; García, H.; Goicochea, E.; Mendoza Hernández, Nilda; Jiménez Despaigne, Sonia
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
D-004, a lipid extract of royal palm (Roystonea regia) fruits that contains a reproducible mixture of fatty
acids,
been
shown
prevent
and phenylephrine-induced
prostate hyperplasia
D-004,has
a lipid
extract
of to
royal
palm testosterone
(Roystonea regia)
fruits that contains a reproducible
mixture ofinrodents.
fatty
Thisstudyinvestigatedthe
oral toxicityand
of D-004
in rats. Rats fromprostate
both sexes
were randomized
acids, has been shown to long-term
prevent testosterone
phenylephrine-induced
hyperplasia
into
four groups
(20 rats sex/group): long-term
a control and
treated
withinD-004
(800,from
1500both
or 2000
inrodents.
Thisstudyinvestigatedthe
oral three
toxicity
of D-004
rats. Rats
sexesmg/kg/day,
were
respectively).
Atfour
studycompletion,
were sacrificed
under
of orblood
randomized into
groups (20 rats rats
sex/group):
a control and
threeanesthesia.
treated with Determinations
D-004 (800, 1500
biochemical
andrespectively).
hematological
parameters andratsorgan
weight were
Also, Determinations
necropsy and
2000 mg/kg/day,
At studycompletion,
were sacrificed
underdone.
anesthesia.
histopathological
studies
performed.
Four of 160
diedweight
before were
studydone.
completion.
No clinical
of blood biochemical
and were
hematological
parameters
andrats
organ
Also, necropsy
andsigns
of
toxicity were studies
observed
throughout
study.
and before
water study
consumption,
bodyweight,
blood
histopathological
were
performed.the
Four
of 160Food
rats died
completion.
No clinical signs
biochemical
andobserved
hematological
parameters,
organ
weight
ratios consumption,
and histopathological
findings
were similar
of toxicity were
throughout
the study.
Food
and water
bodyweight,
blood
in
control and
treated
groups. The
histological
lesions
found
in and
treated
animals are commonly
present in
biochemical
and
hematological
parameters,
organ
weight
ratios
histopathological
findings were
this
specie
and strain
according
to literature
and our historical
data.inIntreated
conclusion,
long-term
(12 months)
similar
in control
and treated
groups.
The histological
lesions found
animals
are commonly
oral
treatment
of rats and
with strain
D-004according
(800-2000tomg/kg/day)
didour
nothistorical
show evidences
of D-004- related
toxicity
present
in this specie
literature and
data. In conclusion,
long-term
under
our conditions.
The highest
doseD-004
tested(800-2000
(2000 mg/kg)
was a no-observed
effect
level in this
(12 months)
oral treatment
of rats with
mg/kg/day)
did not showadverse
evidences
of D-004related toxicity under our conditions. The highest dose tested (2000 mg/kg) was a no-observed adverse
study.
Key words: toxicity,D-004, lipid extract, Roystonea regia fruit, Sprague Dawley rat
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-023 Reproductive Toxicity of D-004, a lipid extract from Roystonea regia fruits, in
Sprague Dawley rats
Gutiérrez Martínez, Ariadne; Pardo, B.; Gámez Menéndez, Rafael; Mas Ferreiro, Rosa María; Noa Puig,
Miriam; Marrero, G.; Arencibia Arrebola, Daniel Francisco; García, H.; Curveco, Dayisell Lázara
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
mixture
of of
free
fatty
acids,
D-004 is aa lipid
lipid extract
extractof
ofroyal
royalpalm
palm(Roystonea
(Roystonearegia)
regia)fruits
fruitsthat
thatconsists
consistsinina a
mixture
free
fatty
acids,
areare
thethe
most
abundant;
that prevents
prostate
hyperplasia
wherein oleic,
oleic, lauric,
lauric,palmitic,
palmitic,and
andmyristic
myristic
most
abundant;
that prevents
prostate
hyperplasia
hashas
shown
to inhibit
the enzyme
activity
of prostate
5- - 5- induced with
with testosterone
testosteroneininrodents.
rodents.D-004
D-004
shown
to inhibit
the enzyme
activity
of prostate
reductase in in
vitro.vitro.
Then, Then,
D-004-impairment
D-004-impairment
of reproductive
of reproductive
toxicity could be
toxicity
expected.
could be expected.
Thisstudyinvestigatedon the possible reproductive toxicity of D-004 administered to fertile Sprague Dawley
Dawley
rats
of both
rats sexes.
of both Rats
sexes.
were
Ratsrandomised
were randomised
into four
into
groups:
four groups:
a control
a control
group,group,
treatedtreated
with the
withvehicle
the
and
vehiclewith
three
andD-004
three at
with
500,
D-004
750 and
at 500,
1 000
750mg/kg/day,
and 1 000respectively.
mg/kg/day, respectively.
Female rats were
Female
treated
rats were
for 15treated
days prior
for
15
days prior
to mating,mating
throughout
mating
and fromtopregnancy
day 21 of
lactation.
Maleadministered
rats were
to mating,
throughout
and from
pregnancy
day 21 of to
lactation.
Male
rats were
10
administered
weeks
before,
and during
mating.
effects
on growth, development,
weeks
before,10and
during
mating.
The effects
onThe
growth,
development,
reproductive reproductive
performance, and
performance,
and
fertility of were
the F1
generation
were
assessed.
No evidence
of D-004-related
toxicity
fertility
of the F1
generation
also
assessed.
No also
evidence
of D-004-related
toxicity
was observed.
Thus,
was observed.
Thus, maternal
weight and
food
consumption,
litter the
size,
survival period
through
theweights of
maternal
body weight
and food body
consumption,
litter
size,
survival through
weaning
and
weaning
weights
of male
female pups
did not
show differences
and and
male
andperiod
femaleand
pups
did not
showand
differences
between
control
and treated between
groups. control
Drug-treated
treated groups'
groups. offspring
Drug-treated
control groups'
comparable
in both physical
control
wereand
comparable
in bothoffspring
physical were
and sensorial
development
and inand
reproduct ive
sensorial
development
andno
in reproductive
performance.
There
no significant
among
F2
performance.
There were
significant differences
among
F2 were
generation
groups differences
in the number
of born
generation
the number
of born pups
pups,survival.
mean pup's
weight andD-004
postnatal
pups
survival. In to rats of
pups,
meangroups
pup's in
weight
and postnatal
In conclusion,
orally
administered
conclusion,
D-004
rats of both
sexes didfunction,
not affect
the fertility
andthat
general
both
sexes did
not orally
affect administered
the fertility andtogeneral
reproductive
which
indicates
it does not alter
reproductive
which indicates
it does
not alterofthe
the
lactation, function,
growth, development
andthat
sexual
maturation
thelactation,
progeny.growth, development and sexual
Key words: toxicity, D-004, Roystonea regia, Sprague Dawley rat
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-024
Teratogenesis of D-004 in rabbits
Gutiérrez Martínez, Ariadne; Gámez Menéndez, Rafael; Mas Ferreiro, Rosa María; García, H.;
Goicochea, E.; Bucarano, I.
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
El D-004 es un extracto lipídico de los
los frutos
frutos de
de la
la palma
palma real
real (Roystonea
(Roystonea regia)
regia) que
que contiene
contiene una
una mezcla
mezcla
reproducible de ácidos grasos, fundamentalmente
fundamentalmente oleico,
oleico, palmítico,
palmítico, láurico
láurico yymirístico.
mirístico.El
Eltratamiento
tratamientooral
oral
significativamente la
la hiperplasia
hiperplasia prostática
prostática inducida
inducidaen
enroedores.El
roedores.Elobjetivo
objetivodel
delestudio
con D-004 previene significativamente
estudio
en evaluarel
teratogénico
en conejos.
Las hembras
preñadasse
consistióconsistió
en evaluarel
potencialpotencial
teratogénico
del D-004del
enD-004
conejos.
Las hembras
preñadasse
distribuyeron
distribuyeron aleatoriamente
enun
tres
grupos:
un control,
tratado (tween
con vehículo
(tween
dos D-004
aleatoriamente
en tres grupos:
control,
tratado
con vehículo
65/agua)
y dos65/agua)
tratadosy con
tratados
con mg/kg).
D-004 (500
y 1000 mg/kg).
Se administró
producto
el día por
18 de
(500
y 1000
Se administró
el producto
desde eleldía
6 hastadesde
el díael18díade6 lahasta
gestación
víalaoral.
gestación
por vía
Se sacrificaron
de se
la examinaron
gestación y sus
fetos de
se anormalidades
examinaron
Se
sacrificaron
lasoral.
hembras
el día 29 delas
la hembras
gestaciónely día
sus 29
fetos
en busca
en busca de
anormalidades
externas, viscerales
y esqueléticas.No
huboentre
diferencias
significativas
entre
externas,
viscerales
y esqueléticas.No
hubo diferencias
significativas
los animales
tratados
y los
los
animales
tratadosal yincremento
los controles
cuanto
al madres
incremento
del peso de
madresdurante
y el consumo
de
controles
en cuanto
delen
peso
de las
y el consumo
de las
alimentos
la gestación.
alimentos
Los grupos
tratadosen
y el
comparables
en elde
número
de
Los
gruposdurante
tratadoslaygestación.
el control fueron
comparables
el control
númerofueron
de cuerpos
lúteos, sitios
implantación,
cuerpos lúteos, fetos
sitios vivos,
de implantación,
reabsorciones,
fetos vivos,
de Se
los fetos
y proporción
de loscon
reabsorciones,
peso de los
fetos y proporción
de lospeso
sexos.
encontraron
tres fetos
sexos. Se encontraron
malformaciones
viscerales:
tres fetos
uno con
del malformaciones
grupo control y viscerales:
dos tratados
unocon
del 1000
grupo mg/kg.
control No
y dos
setratados
observaron
con
1000 mg/kg. No se
malformaciones
externas
o esqueléticas
en externas
ninguno odeesqueléticas
los grupos.en Las
ninguno
variaciones
de los grupos.
esqueléticas
Las
observaron
malformaciones
variaciones esqueléticas
observadasno
tuvieron una
observadasno
incidencia estadísticamentesignificativa.Los
tuvieron una incidencia estadísticamentesignificativa.Los
resultados del estudio permiten
concluir
quedella estudio
administración
vía oral
delaD-004
en dosis de
1000
resultados
permitenpor
concluir
que
administración
por500
vía yoral
de mg/kg
D-004 durante
en dosiseldeperíodo
500 y de
organogénesis no es teratogénica o embriotóxica en conejos.
Key words: teratogenesis, D-004, rabbit
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-025 Effects of D-004 and anti-oxidant and/or anti-inflammatory substances on prostatic
hyperplasia induced by testosterone and phenylephrine in rats
Oyarzábal Year, Ambar; Pérez Guerra, Yohani; Molina Cuevas, Vivian; Jiménez Despaigne, Sonia;
Ravelo Calzado, Yazmin; Mas Ferreiro, Rosa María
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
study
waswas
to investigate
the effects
of D-004,
and/or anti-inflammatory
The aim
aimofofthis
this
study
to investigate
the effects
of antioxidant
D-004, antioxidant
and/or anti-inflammatory
prostate hyperplasia
hyperplasia (PH)
(PH) induced
induced by
bytestosterone
testosterone(T)
(T)and
andphenylephrine
phenylephrine(PHE)
(PHE)ininrats.
rats.Two
substances on prostate
Two experiments
conducted.
First experiment:
Rats randomized
were randomized
into seven
a negative
experiments
were were
conducted.
First experiment:
Rats were
into seven
groups:groups:
a negative
control
control
and
six and
groups
six injected
groups injected
with T; awith
positive
T; a positive
control and
control
five and
were
fivetreated
were treated
with D-004
with(400
D-004
mg/kg),
(400 mg/kg),
grape seed
grape seed
extract
(GSE)
extract
(250 (GSE)
mg/kg),
(250
vitamin
mg/kg),
E (VE)
vitamin
(250
E (VE)
mg/kg),
(250ibuprofen
mg/kg), ibuprofen
(200 mg/kg)
(200ormg/kg)
celecoxib
or celecoxib
(50 mg/kg).
(50 mg/kg).
Second
experiment:
Second experiment:
Rats were randomized
Rats were randomized
into eight groups:
into eight
a negative
groups:vehicle
a negative
control
vehicle
and seven
controlgroups
and
seven groups
injected
with PHE:
injected
a positive
with PHE:
control,
a positive
three treated
control, with
threeD-004
treated
(200,
with400
D-004
and(200,
800 mg/kg)
400 andand
800three
mg/kg)
others
and
others (0.4
with mg/kg),
tamsulosin
mg/kg),
GSE
mg/kg)
and VE
(250 mg/kg),
In the
with three
tamsulosin
GSE(0.4
(250
mg/kg)
and(250
VE (250
mg/kg),
respectively.
In respectively.
the first experiment:
first experiment:
Oral
of D-004,
of any other
treatment,
significantly
prostate
Oral
administration
ofadministration
D-004, not of any
other not
treatment,
significantly
reduced
prostatereduced
weigh and
prostate
weigh and prostate
weigh/body
weighto ratio
as compared
the positive
control.
D-004,
GSE and
VE, not
weigh/body
weigh ratio
as compared
the positive
control.toD-004,
GSE and
VE, not
ibuprofen
or celecoxib,
ibuprofensignificantly
or celecoxib,
lowered
significantly
MDA
values. inhibitions
In contrast,
inhibitions
lowered
prostate
MDA
values. Inprostate
contrast,
significant
ofsignificant
prostate MPO
activityof were
prostate MPO
GSE, not
ibuprofen
andSecond
celecoxib,
not with VE.
Second
achieved
with activity
D-004, were
GSE,achieved
ibuprofenwith
andD-004,
celecoxib,
with VE.
experiment:
D-004
(200-800
experiment:
D-004significantly
(200-800 mg/kg)
significantly
voided
per total
micturition
andlowered
mg/kg)
increased
volumeincreased
voided per
micturitionvolume
(VM) and
urinary
volume(VM)
(UTV),
urinary
total MDA
volume
significantly
MDA in prostate
and bladder
significantly
in (UTV),
prostatelowered
and bladder
homogenates,
and reduced
carbonylhomogenates,
groups (GC) and
levelsreduced
only in
carbonyl
groups
(GC) levels
only in significantly
the prostate.VM
Tamsulosin
significantly
VMvariables.
and UTV,GSE
but and
the
prostate.
Tamsulosin
increased
and UTV, increased
but unchanged
oxidative
unchanged
oxidative
variables.
GSEVE,
andnot
VEGSE,
unchanged
the UTV,
whereas VE,
not GSE,the
modestly
but
VE
unchanged
the UTV,
whereas
modestly
but significantly
attenuated
PHE-induced
significantly
the PHE-induced
decrease
of D-004
VM. In (400
conclusions,
treatment
with
D-004 (400
decrease
of attenuated
VM. In conclusions,
oral treatment
with
mg/kg), oral
but not
with the
antioxidant
and
mg/kg), but not with
the antioxidant
anti-inflammatory
tested,
prevented
T-induced
in
anti-inflammatory
substances
tested,and
prevented
T-induced substances
PH in rats, so
that the
preventive
effect ofPH
D-004
rats,
on
this
somodel
that the
does
preventive
not seem
effect
to beofassociated
D-004 on this
to its
model
antioxidant
does not
or anti-inflammatory
seem to be associated
effects.
to The
its antioxidant
single oral
or anti-inflammatory
administration
of D-004
effects.
(200-800
The single
mg/kg)oral
was
administration
the only treatment
of D-004
that(200-800
ameliorated
the urodynamic
mg/kg)
was the onlychanges
and
reduced
oxidative
variables in
the prostate
of rats with
PHE-induced
treatment
thatincreased
ameliorated
the urodynamic
changes
and reduced
increased
oxidativePH.
variables in the
Key words: anti-inflammatory; antioxidant; d-004; prostate hyperplasia; urodynamic changes
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-026 Effects of oral treatment (60 days) with D-004, a lipidic extract from Roystonea regia
fruits, on the rat prostate hyperplasia and oxidative markers
Oyarzábal Year, Ambar; Jiménez Despaigne, Sonia; Pérez Guerra, Yohani; Molina Cuevas, Vivian; Mas
Ferreiro, Rosa María; Curveco, Dayisell Lázara
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
Short-term (14 days) treatment with
with D-004,
D-004, aa lipid
lipid extract
extractfrom
from Roystonea
Roystonea regia
regiafruits,
fruits,has
hasbeen
beenshown
showntoto
prevent testosterone (T)-induced prostate hyperplasia (PH) and increased
increased oxidative stress
stress in
in rat
rat prostate,
prostate,
but no
no study
study has
hasdocumented
documentedif ifsuch
sucheffects
effectspersist
persist
after
after
longer
longer
treatment.
treatment.
ThisThis
study
study
investigated
investigated
the the
persistence of the
the effects
effects of
ofD-004
D-004orally
orallygiven
givenfor
for6060days
daysforforpreventing
preventingprostate
prostate
enlargement
enlargementand
and
increase
increase
of
oxidative
of oxidative
markers in
markers
rats with
in T-induced
rats with T-induced
PH. Rats were
PH. Rats
randomized
were randomized
into three groups:
into three
a negative
groups: acontrol
negative
and
two T-injected
control andthat
tworeceived
T-injectedorally
that vehicle
received(positive
orally vehicle
control)
(positive
or D-004
control)
(400 or
mg/kg/day),
D-004 (400respectively.
mg/kg/day),
Prostate
weights
respectively.
of positive
Prostate
controls
weights
significantly
of positive
andcontrols
markedly
significantly
increasedand
overmarkedly
the time,increased
while persistent
over
the
while persistent
reductions
suchinincreases
were seen
D-004-treated
rats.
and time,
significant
reductionsand
of significant
such increases
were of
seen
D-004-treated
rats. in
Also,
D-004 significantly
Also, D-004
of prostate
reduced
thesignificantly
T-induced reduced
increasetheofT-induced
prostateincrease
conjugated
diene conjugated
generation diene
and generation
sulphydril and
groups
sulphydril groupsachieving
concentrations,
achieving
a complete
reduction
from the
day 30
startingConcluding,
the
concentrations,
a complete
reduction
from the
day 30 after
starting
theafter
treatment.
treatment.
the
effects Concluding,
of oral treatment
the effects
with D-004
of oral
(400
treatment
mg/kg/day)
with on
D-004
T-induced
(400 mg/kg/day)
prostate enlargement
on T-inducedand
prostate
increased
prostate oxidative markers persisted over 60 days of treatment.
Key words: d-004, prostatic hyperplasia, oxidative markers, roystonea regia, testosterone
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-027
Characterization of vegetable material and oil obtained from Acrocomia crispa fruits
Sierra Pérez, Roxana de la Caridad; Vicente Murillo, Roxana; González Canavacciolo, Víctor L.; Morales
Rico, Carmen Luisa; Rodríguez Leyes, Eduardo Antonio; Marrero Delange, David; Tamame Tirado, Dayana
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
El D005, nuevo
nuevo ingrediente
ingrediente activo
activo antiinflamatorio,
antiinflamatorio, se
seobtiene
obtiene aapartir
partirdel
delaceite
aceitede
delos
losfrutos
frutosmaduros
madurosdede
Acrocomia crispa. Al
Al no
no existir
existiruna
unametodología
metodología para
paraelelprocesamiento
procesamientodedeestos
estos
frutos
frutos
y no
y no
encontrarse
encontrars e
vegetal
o del
aceite
nosnos
reportes sobre las
las características
características físico-químicas
físico-químicas oolalacomposición
composicióndel
delmaterial
material
vegetal
o del
aceite
propusimos como
tratamiento
de de
los los
frutos
y caracterizar
el material
como objetivo
objetivo establecer
establecerelelmétodo
métododede
tratamiento
frutos
y caracterizar
el material
eraera
a temperatura
ambiente
a laa sombra
vegetal y el
el aceite.
aceite. Se
Seencontró
encontróque
quelalamejor
mejorforma
formadedesecado
secado
a temperatura
ambiente
la sombra
molino de
de martillos.
martillos. Ocho
Ocho lotes
lotes de
de material
material veget al
durante 25 días, y que la molienda debía realizarse con molino
vegetal
se
caracterizaron
se caracterizaron
en cuanto
en cuanto
a humedad
a humedad
residual
residual
(< 8 %),
(< 8 contenido
%), contenido
de cenizas
de cenizas
(totales:
(totales:
2,302,303,87%;
3,87%; insolubles
insolubles
en HCl: en
0,70-0,97%;
HCl: 0,70-0,97%;
solublessolubles
en agua:
en0,44-1,59%),
agua: 0,44-1,59%),
contenido
contenido
de aceite
de (13-18
aceite (13-18
%) y contenido
%) y
contenido
de
ácidos grasos
de ácidos
(totales:
grasos92-96
(totales:
% y libres:
92-96 %
1-3y %)
libres:
en el1-3
aceite.
%) enAdemás,
el aceite.seAdemás,
obtuvieron
se ocho
obtuvieron
lotes de
ocho
aceite
lotes
a
partir
de aceite
de cada
a partir
lote de
decada
material
lote de
vegetal
material
con
vegetal
un rendimiento
con un rendimiento
superiorsuperior
al 11 %,
al 11los%,cuales
los cuales
fueron
fueron caracterizados
caracterizados
encontrándose
encontrándose
que todos
que fueron
todos fueron
líquidoslíquidos
oleosos
oleosos
traslúcidos
traslúcidos
color amarillo-naranja
color amarillo-naranja
y olor
y olor dulce,
dulce,
con pérdidas
con pérdidas
por secado
por secado
< 5 %,
< 5densidad
%, densidad
relativa
relativa
(0,88-0,91
(0,88-0,91
g/ml)g/ml)
e índice
e índice
de de
refracción
refracción
(1,449(1,449-1,457).
1,457).
El índice
El de
índice
saponificación
de saponificación
estuvo estuvo
entre 211-239,
entre 211-239,
el contenido
el contenido
de material
de material
no saponificable
no
< 1 %,
saponificable
el
contenido de
< 1ácidos
%, el grasos
contenido
totales
de ácidos
90 %grasos
y elevados
totalesíndices
90 %de
y elevados
acidez (48-345)
índices lo
deque
acidez
concuerda
(48-345)con
lo que
un
altoconcuerda
contenido con
de ácidos
un altograsos
contenido
libresde(9-60
ácidos
%).grasos
Se realizó
libresun(9-60
extracto
%). Se
metanólico
realizó un
a extracto
un lote de material
metanólico
vegetal
encontrándose
a un lote de un
material
0,007 vegetal
% de polifenoles
encontrándose
expresado
un 0,007
como
%pirogalol.
de polifenoles expresado como
Key words: acrocomia crispa, caracterización de aceite, material vegetal
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-028
Characterization of D005 a lipidic extract from Acrocomia crispa fruits
Sierra Pérez, Roxana de la Caridad; Vicente Murillo, Roxana; González Canavacciolo, Víctor L.; Morales
Rico, Carmen Luisa; Rodríguez Leyes, Eduardo Antonio; Marrero Delange, David; Tamame Tirado, Dayana
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
Los fitofármacos, representan
representan una
una parte
parte importante
importante del
delcomercio
comerciodedemedicamentos,
medicamentos,requiriendo
requiriendononosolo
solo
de procesos de
de obtención
obtención reproducibles
reproducibles sino
sinotambién
tambiéndedecontroles
controlesdede
calidad
calidad
queque
permitan
permitan
verificar
verificar
el el
D005,
es es
un un
nuevo
ingrediente
activo
compuesto
por una
cumplimiento de
de los
los atributos
atributosdeseados.
deseados.ElEl
D005,
nuevo
ingrediente
activo
compuesto
por una
mezcla de ácidos grasos libres extraídos yy purificados a partir
partir de
de los
los frutos
frutos de
de A.
A.crispa
crispa con
con potente
potente efecto
efecto antiinflamatorio.
Tomando
en consideración
lo el
dicho,
el trabajo
tuvoobjetivo
como objetivo
caracterizar
antiinflamatorio.
Tomando
en consideración
lo dicho,
trabajo
tuvo como
caracterizar
los loteslos
de
lotes de
D005,
para establecer
los intervalos
ense
losencuentran
que se encuentran
sus principales
características.
Se
D005,
para
establecer
los intervalos
en los que
sus principales
características.
Se encontró
encontró
eran oleosos
líquidos oleosos
traslúcidos
color naranja-rojizo
y oloragradable.
dulce
que
todosque
los todos
lotes los
eranlotes
líquidos
traslúcidos
de colorde
naranja-rojizo
y olor dulce
Los
agradable. Los
de obtención
partir del aceite
estuvieron
del 70por
%,secado
las
rendimientos
derendimientos
obtención a partir
del aceitea estuvieron
por encima
del 70por
%,encima
las pérdidas
<1%,
pérdidas
porrelativa
secadoentre
<1%,0,88-0,91
la densidad
relativa
entre de
0,88-0,91
g/mL,
el índice
refracción
entre
la densidad
g/mL,
el índice
refracción
osciló
entre de
1,446
y 1,453,osciló
el contenido
1,446
y 1,453,
contenido de< material
no saponificable
< 2 encima
%, el índice
de acidez
por encima
de 350,
el
de
material
no el
saponificable
2 %, el índice
de acidez por
de 350,
el contenido
de ácidos
grasos
contenido
de ácidos
libres superior
al 90 %.de
La ácidos
composición
de ácidos
grasos,
mostró
libres
superior
al 90 grasos
%. La composición
individual
grasos,individual
mostró que
el D005
presenta
como
que el D005 presenta
como los
componentes
mayoritarios
ácidos
(C12:0=
34,75 %) y %)
mirístico
componentes
mayoritarios
ácidos láurico
(C12:0= los
34,75
%) láurico
y mirístico
(C14:0=13,22
y oleico
(C14:0=13,22
(C18:1=
30,41 %)
%)yseguido
oleico (C18:1=
de los ácidos
30,41 %)
palmítico
seguido(C16:0=
de los ácidos
8,94 %),
palmítico
esteárico
(C16:0=
(C18:0=
8,94
3,62
%),%),
cáprílic o
esteárico
(C18:0= 3,62 %),
(C8:0=1,04%),
cáprico
cáprílico
(C10:0=2,29
(C8:0=1,04%),
%), palmitoleico
cáprico (C10:0=2,29
(C16:1= 0,07
%), palmitoleico
%). La determinación
(C16:1= 0,07
de los
%).intervalos
La
en
los que se encuentran
estos en
parámetros
permitirá
establecer
las especificaciones
de calidad para
determinación
de los intervalos
los que se
encuentran
estos parámetros
permitirá establecer
las este
ingrediente activo.
Key words: acrocomia crispa, d005, extracto lipídico
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-029
Determination of the oil and fatty acid content in fruits of palms that grown in Cuba
Vicente Murillo, Roxana; Rodríguez Leyes, Eduardo Antonio; González Canavacciolo, Víctor L.; Marrero
Delange, David; Sierra Pérez, Roxana de la Caridad; Morales Rico, Carmen Luisa
Centro Nacional de Investigaciones Científicas (CNIC),
[email protected]
Aunque
se encuentran
encuentran ampliamente
Aunque se
ampliamente distribuidas
distribuidas en
en Cuba
Cuba yy hay
hay varios
varios reportes
reportes de
de sus
sus usos
usos etnomédicos,
las
palmas cubanas
han cubanas
sido poco
sin embargo,
cuenta en
la gran
variedad
etnomédicos,
las palmas
hanestudiadas;
sido poco estudiadas;
sinteniendo
embargo,enteniendo
cuenta
la gran de
palmas
existentes
en
Cuba
y
lo
factible
que
pudiera
resultar
el
empleo
de
sus
frutos
para
la
obtención
variedad de palmas existentes en Cuba y lo factible que pudiera resultar el empleo de sus frutos para lade
productos
naturales
con
usos nutracéuticos
y/o farmacéuticos;
resulta interesante
realizar realizar
estudios
obtención de
productos
naturales
con usos nutracéuticos
y/o farmacéuticos;
resulta interesante
fitoquímicos
preliminares
a algunasa de
las especies
nativas o nativas
introducidas
del país. del
Parapaís.
la realización
estudios fitoquímicos
preliminares
algunas
de las especies
o introducidas
Para la de
este
trabajode
seeste
recolectaron
maduros frutos
frescos
de S. repens,
regia,
Sabal palmetto,
Sabal
realización
trabajo sefrutos
recolectaron
maduros
frescosRoystonea
de S. repens,
Roystonea
regia, Sabal
maritima,
Colpothrinax
Thrinax whritii,
radiata,
Veichia
merrillii,
Chrysalidocarpus
lutescen, Wotyetia
palmetto, Sabal
maritima,whritii,
Colpothrinax
Thrinax
radiata,
Veichia
merrillii, Chrysalidocarpus
bifurcata,
Caryota bifurcata,
urens y Phytosperma
Los frutos
se secaron
a temperatura
a la
lutescen, Wotyetia
Caryota urenselegans.
y Phytosperma
elegans.
Los frutos
se secaron aambiente
temperatura
sombra
20 días,
se molieron
se molieron
sometieron
extracción con
hexano en
extractos
ambientedurante
a la sombra
durante
20 días,y se
y sea sometieron
a extracción
consöxhlet.
hexanoLos
en söxhlet.
lipídicos
se secaron
y se
determinaron
rendimiento el
derendimiento
extracción yde
sus
contenidos
de ácidos
grasos
Los extractos
lipídicos
seles
secaron
y se les el
determinaron
extracción
y sus
contenidos
de
como
metílicos
pormetílicos
cromatografía
de gases. de
Estos
extractos
presentaron
rendimientos de
ácidos ésteres
grasos como
ésteres
por cromatografía
gases.
Estos extractos
presentaron
extracción
entre
0,6 y 25,5 %;
contenido
de %;
ácidos
grasosdetotales
hasta
92,5 58,4
% y hasta
contenidos
rendimientos
de extracción
entre
0,6 y 25,5
contenido
ácidosdesde
grasos58,4
totales
desde
92,5
de
libres de
entre
1,9 ylibres
70,2 entre
%. La1,9
composición
de composición
ácidos grasos
como ácidos
mayoritarios
% yácidos
contenidos
ácidos
y 70,2 %. La
depresentó
ácidos grasos
presentó
como
en
las todas
las especies
el palmítico
(9,2-52,2 el
%)palmítico
y el oleico(9,2-52,2
(9,6-42,7);
que
los ácidos
ácidos
mayoritarios
en lasestudiadas
todas las especies
estudiadas
%) mientras
y el oleico
(9,6-42,7);
linoleico
3,4ácidos
%) y esteárico
fueron
los minoritarios.
Losfueron
ácidos
mirísticoLos
y linoleico
mientras (0,1
que -los
linoleico(1,7
(0,1-6,1
- 3,4%)%)
y esteárico
(1,7 -6,1 %)
losláurico,
minoritarios.
ácidos
fueron
en unasfueron
especies
y minoritarios
paraespecies
otras. El
presente trabajo
láurico,mayoritarios
mirístico y linoleico
mayoritarios
en unas
y minoritarios
para permitió
otras. Elcaracterizar
presente
las
fracciones
de las
once
especieslipídicas
de arecaceas
la isla, lopresentes
cual contribuyó
trabajo
permitió lipídicas
caracterizar
fracciones
de once presentes
especies deenarecaceas
en la isla,al
conocimiento
de
algunas
de
las
principales
características
físicas
y
químicas,
con
vistas
a
su
lo cual contribuyó al conocimiento de algunas de las principales características físicas y químicas, posible
con
empleo en la industria farmacéutica.
Key words: palmas cubanas, aceite, ácidos grasos, frutos de arecaceae
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-030
Fatty acids profile of two species of Lamiaceae that grown in Cuba
Marrero Delange, David; Morales Rico, Carmen Luisa; González Canavacciolo, Víctor L.; Sierra Pérez,
Roxana de la Caridad; Rodríguez Leyes, Eduardo Antonio; Vicente Murillo, Roxana
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
The Lamiaceae family has been characterized by the occurrence of unsaturated acids
acids in their
their seed oils.
The importance of
of such
such acids
acids as
as chemotaxonomic
chemotaxonomic markers,
markers,for
forthe
thecosmetic,
cosmetic,nutritional
nutritionaland
andmedicinal
medicinal
also been
been demonstrated.
demonstrated. Salvia
Salvia coccinea
coccineaL.,
L.,known
knownasasbanderilla
banderillaand
andLeonotis
Leonotisnepetaefolia
industries has also
nepetaefolia
(L.) R.Br.,
knowndeasSan
Bastón
de Sanare
Francisco
are two
thisare
family
and cultivated
are mainly
(L.) R.Br., known
as Bastón
Francisco
two members
of members
this familyofand
mainly
cultivated
Cuba as ornamental
plants.
The of
purpose
of this
was to determine
acid
in
Cuba asinornamental
plants. The
purpose
this work
waswork
to determine
the fatty the
acidfatty
composition
of
composition
of seed
oil of these
Seeds were
collected,
milled and
seed
oil of these
species.
Seedsspecies.
were collected,
milled
and extracted
withextracted
hexane. with
Thehexane.
extractsThe
were
extractsand
werethe
filtered
andwas
the evaporated
solvent was under
evaporated
under
reduced
in a rotary evaporator.
filtered
solvent
reduced
pressure
in pressure
a rotary evaporator.
Organolept ic
Organoleptic characteristics
characteristics
and extractionand
yields
extraction
of the oils
yields
were
of the
determined.
oils were The
determined.
content of
The
fatty
content
acids,ofanalysed
fatty acids,
as
analysed
methyl
esters,
as methyl
were determined
esters, werebydetermined
GC-FID and
byGC/MS.
GC-FID The
andmain
GC/MS.
fatty The
acidsmain
found
fatty
in the
acids
oil of
found
S. coccinea
in the oil
of S. coccinea
were
linolenic (33.1%),
were linolenic
linoleic(33.1%),
(25.2%)linoleic
and oleic
(25.2%)
(13.3%)
andacids;
oleic whereas
(13.3%) acids;
in L. nepetaefolia
whereas inoil
L. were
nepetaefolia
found
oil were
oleic
(43.2%),
found laballenic
oleic (43.2%),
(18.4%),
laballenic
palmitic
(18.4%),
(15.0%),
palmitic
and linolenic
(15.0%),
(13.4%)
and linolenic
acids. Other
(13.4%)
fatty
acids.
acidsOther
were also
fatty
found
but inalso
minor
proportions.
Theproportions.
results of this
areofathis
contribution
the chemicaltocomposition
acids were
found
but in minor
Thestudy
results
study are to
a contribution
the chemical
knowledge of both species and suggest that such oils could be used in several industries.
Key words: salvia coccinea, leonotis nepetaefolia, lamiaceae, fatty acid, gas chromatography
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-031
Study of the methanolic extract from Terminalia catappa leaves
Marrero Delange, David; Morales Rico, Carmen Luisa; González Canavacciolo, Víctor L.; Vicente
Murillo, Roxana
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
Varias de
delas
lasespecies
especies
deldel
género
género
Terminalia
Terminalia
(Combretaceae)
(Combretaceae)
han demostrado
han demostrado
internacionalmente
internacionalment e
cardioprotectoras,
hipolipemiantes,
vasodilatadoras,
diuréticas,
propiedades farmacológicas
farmacológicascomo
como
cardioprotectoras,
hipolipemiantes,
vasodilatadoras,
diuréticas,
propiedades
antioxidantes, gastroprotectoras,
gastroprotectoras, hepatoprotectoras,
hepatoprotectoras, antiinflamatorias
antiinflamatorias y yanalgésicas.
analgésicas.Estas
Estas
propiedades
han sido atribuidas
glicósidos,
encontrados
en en
las las
hojas
y la y la
atribuidas fundamentalmente
fundamentalmente aalos
lospolifenoles
polifenolesy sus
y sus
glicósidos,
encontrados
hojas
catalogada
corteza. Teniendo en
en cuenta
cuenta además,
además, que
que en
enCuba
Cubalalaespecie
especieTerminalia
TerminaliaCatappa
Catappaeses
catalogadacomo
como
su su
composición
química
y estudios
una planta invasora
invasora yyque
queexisten
existenpocos
pocostrabajos
trabajosrelacionados
relacionadoscon
con
composición
química
y estudios
farmacológicos, se llevó aa cabo
cabo un
un estudio
estudio preliminar
preliminar para
paradeterminar
determinarácidos
ácidospolifenólicos
polifenólicosenenelelextracto
extracto
molidas,
desgrasadas
concon
hexano
y y
metanólico de
de sus
sus hojas.
hojas. Las
Lashojas
hojasfueron
fueronrecolectadas,
recolectadas,secadas,
secadas,
molidas,
desgrasadas
hexano
posteriormente extraídas con metanol en un baño ultrasónico.
ultrasónico. El
El extracto
extracto se
se filtró
filtró yy el
el disolvente
disolvente se
seeliminó
eliminó
vacío.
El extracto
seco
se hidrolizó
y se extrajo
con acetato
de etilo.
Se determinó
el
al
vacío.al El
extracto
seco se
hidrolizó
y se extrajo
con acetato
de etilo.
Se determinó
el rendimiento
de
rendimiento las
de extracción,
las características
y los totales
polifenoles
totales el
mediante
método
extracción,
características
organolépticasorganolépticas
y los polifenoles
mediante
métodoelde
Follinde Follin-Ciocalteu,
como la composición
química
porprevia
CG/EM
previa formación
de derivados
TMS. Se
Ciocalteu,
así como así
la composición
química por
CG/EM
formación
de derivados
TMS. Se obtuvo
un
obtuvo de
líquido
un color
líquidopardo
de color
rojizo
pardo
oscuro
rojizo
de olor
oscuro
característico
de olor característico
y con un rendimiento
y con un rendimiento
de extracción
de extracción
de 10,8%. El
de 10,8%. El
contenido
total
contenido
de polifenoles
total de polifenoles
fue 184,6fue
(mg
184,6
Pirogalol/100g
(mg Pirogalol/100g
Extracto)
Extracto)
y como
y como
ácidos
ácidos
polifenólicos
polifenólicos mayoritarios
mayoritarios
se encontraron
se encontraron
el ácido gálico
el ácido(3,4,5-trihidroxibenzoico),
gálico (3,4,5-trihidroxibenzoico),
ácido vanílico
ácido vanílico
(3- metoxi-4(3metoxi-4-hidroxibenzoico),
hidroxibenzoico),
ácido 3,4-dihidroxibenzoico,
ácido 3,4-dihidroxibenzoico,
ácido 2,5-dihidroxi-benzoico,
ácido 2,5-dihidroxi-benzoico,
ácido 4ácido
hidroxibenzoico
4y
hidroxibenzoico
ácido benzoico.
También
se estructuras
detectaron otras
estructuras
polifenoles.
ácido
benzoico. yTambién
se detectaron
otras
relacionadas
conrelacionadas
polifenoles. con
La presencia
de
La presencia
de estos
compuestos
justificar las
propiedades
medicinales
atribuidas
estos
compuestos
pudiera
justificarpudiera
las propiedades
medicinales
atribuidas
a esta
especie, aaesta
la vez que
servirían de base para continuar con futuras pruebas farmacológicas que avalen sus usos etnomédicos.
Key words: terminalia catappa, combretaceae, ácido gálico, polifenoles, cg/em, tms
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-032 Determination of arsenic in natural products by hydride generation - atomic
absorption spectrometry
Luna Saucedo, Bárbara E.; Quevedo Álvarez, Odalys; Fernández Ruíz, Rosabel; Gómez Pascual,
Jorge; Estrada la Rosa, Tamara; Sánchez Sotolongo, Elsa
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
The national and international
international regulator
regulator organisms
organisms demand,
demand, for
for the
theuse
useof
ofnatural
naturalproducts
productslike
likenutritional
nutritional
of of
some
toxic
elements
for men,
suchsuch
as the
supplements or
or drugs,
drugs, to
toknow
knowwith
withaccuracy
accuracythe
thecontent
content
some
toxic
elements
for men,
as the
Arsenic (As). The Policosanol, Prevenox
Prevenox and
and Abexol
Abexol are
are actives
actives pharmaceuticals
pharmaceuticals ingredients
ingredients extracted
extractedofof
the wax of the sugar cane
cane and
and bees
bees and
and they
they are
are constituted
constitutedby
bymixtures
mixturesofofalcohols
alcoholsororaliphatic
aliphaticacids,
acids,both
both high
with
with high
molecular
molecular
weight,
weight,
showing
showing
cholesterol
cholesterol
lowering,
lowering,
antioxidant
antioxidant
andand
gastroprotective
gastroprotective
effects.
effects.
In the
In
the present
present
study,
study,
a microwave-assisted
a microwave-assisted
digestion
digestion
technique
technique
(HNO3(HNO3H2O2,
H2O2,
EPA
EPA
3052)
3052)was
wasemploy
employfor
forthe
the determination
determination
of total
of total
arsenic
arsenic
in in
thethe
three
threematrices
matricesofofnatural
natural products
products by
by Hydride Generation
Generation --Atomic
Atomic
Absorption Spectrometry.
determination
waswas
evaluated
by the
additions
Spectrometry. The
Theaccuracy
accuracyofofthethe
determination
evaluated
by standard
the standard
additions
by three
three samples
samples between
between 95%
95% and
and105
105. .The
Thedetection
detectionlimit
limitwas
method. The recoveries were satisfactory by
was 0.002
µg.g-1.
content
of in
Asthe
in the
three
analysed
natural
products
were
under
method
0.002
µg.g-1.
The The
content
of As
three
analysed
natural
products
were
under
thethe
method
detection
detection
limit
as the established
of the
United
States Pharmacopeia
Ed. for
similar
limit
as well
asas
thewell
established
limit of thelimit
United
States
Pharmacopeia
33 Ed. for 33
similar
products.
Key words: arsenic, antioxidants, hydride generation, atomic absorption spectrometry
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-033 Characterization of by-product from the obtaining process of propolis for its possible
use as nutritional supplement
Martínez Rojas, José Manuel; Cabrera Rabí, Leila; Fernández, Jaquelin; Urra, Caridad; Fajardo, Mario; M.
Pérez, Adolfo; Falero Morejón, Alina
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
El propóleo es un producto natural que las abejas elaboran a partir de exudados de diversas plantas. Es
El propóleo es un producto natural que las abejas elaboran a partir de exudados de diversas plantas. Es
usado por las abejas para recubrir las paredes de la colmena y mantenerla libre de bacterias. Está
usado por las abejas para recubrir las paredes de la colmena y mantenerla libre de bacterias. Está
compuesto básicamente por sustancias resinosas procedentes de distintas especies vegetales con un
compuesto básicamente por sustancias resinosas procedentes de distintas especies vegetales con un
50% de bálsamos y resinas, 35% de cera, 10% de aceites esenciales y un 5 % de polen. Este material
50% de bálsamos y resinas, 35% de cera, 10% de aceites esenciales y un 5 % de polen. Este material
resinoso es sometido a una extracción hidroalcohólica para la obtención de tintura de propóleos y extracto
resinoso es sometido a una extracción hidroalcohólica para la obtención de tintura de propóleos y
blando. Como subproducto de este proceso se obtiene un residuo ceroso que se saponificó, se extrajo
extracto blando. Como subproducto de este proceso se obtiene un residuo ceroso que se saponificó, se
con acetona y se purificó empleando seis combinaciones de hexano-acetona. Los mejores resultados se
extrajo con acetona y se purificó empleando seis combinaciones de hexano-acetona. Los mejores
encontraron después de dos purificaciones
con hexano y tres con acetona, obteniéndose una
resultados se encontraron después de dos purificaciones con hexano y tres con acetona, obteniéndose
concentración de 94 % de una mezcla de alcoholes de alto peso molecular, donde predominan los
una concentración de 94 % de una mezcla de alcoholes de alto peso molecular, donde predominan los
alcoholes C30 y C32. El objetivo de este trabajo fue caracterizar este residuo ceroso y evaluar su posible
alcoholes C30 y C32.
uso como suplemento alimentario, teniendo en cuenta las propiedades conocidas de los alcoholes
El objetivo de este trabajo fue caracterizar este residuo ceroso y evaluar su posible uso como
presentes en la cera de abejas y la cantidad de subproducto generado en la obtención de tintura de
suplemento alimentario, teniendo en cuenta las propiedades conocidas de los alcoholes presentes en la
propóleos y extracto blando.
cera de abejas y la cantidad de subproducto generado en la obtención de tintura de propóleos y extracto
Key words:
propolis, nutritional supplement, wax
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-034 Study of the behavior of oral Oleozón manufactured in a new production place
under conditions of accelerated stability
Bécquer Romagoza, Clara; Mosquera Sierra, Javier; Rodríguez Lavañino, Jean M.; Sánchez Sánchez,
Dennis; Mediacejas Maletá, Yuleikis; Kindelán Mallet, Mylene
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
Introducción: El
Elaceite
aceitedede
girasol
girasol
ozonizado
ozonizado
OLEOZON®
OLEOZON®
Oral Oral
es un es
medicamento
un medicamento
que presenta
que presenta
excelentes propiedades en
en la
la parasitosis
parasitosis por
por Giardia
Giardia Lamblia.
Lamblia.En
Eneste
estetrabajo
trabajose
sepresenta
presentaelelestudio
estudiodede
estabilidad acelerada de tres lotes de producción de OLEOZON® Oral a partir de la
la puesta
puesta en
en marcha
marcha de
de
la planta de
de producción
producción de
deaceite
aceiteozonizado
ozonizadoCNIC
CNICy yteniendo
teniendoenencuenta
cuenta
laslas
indicaciones
indicaciones
deldel
CECMED.
CECMED.
Materiales y Métodos: Se
Se utilizaron
utilizaron frascos
frascos plástico
plásticode
debaja
bajadensidad
densidady yde
devidrio.
vidrio.Los
Loslotes
lotesenenestudio
estudiosese
mantuvieron con las
las condiciones
condiciones requeridas
requeridas aauna
una Temperatura
Temperatura de
de(25
(25±2)0C
±2)0C YY60%
60%dede
Humedad.
Humedad.SeSe
analizaron durante tiempo 0, 1, 2, 3 y 66 meses
meses las
las características
características de
de calidad:
calidad: índice
índice de
de peróxido,
peróxido, índice
índice de
de
acidez,
concentración
aldehídos,viscosidad,
viscosidad,características
características organolépticas
organolépticas yy límite microbiano.
acidez,
concentración
dede
aldehídos,
microbiano. Se
Se
Origin
para
el análisis
de los
Resultados
y
utilizó computadora
computadora yyelelprograma
programaestadístico
estadístico
Origin
para
el análisis
deresultados.
los resultados.
Resultados
y
Discusión: Durante los seis meses de mantener la muestra en estudio
estudio acelerada en frascos
frascos de plástico
plástico y
medida
que
transcurre
el tiempo
el valor
de de
de vidrio se
se ha
ha podido
podido apreciar
apreciar que
queelelíndice
índicededeperóxido
peróxidoa a
medida
que
transcurre
el tiempo
el valor
para
cada
unouno
de de
los los
lotes,
la la
concentración en el
el tiempo
tiempo cero
cerodisminuyó
disminuyó, ,elelíndice
índicededeacidez
acidezaumentó
aumentó
para
cada
lotes,
viscosidad y la concentración en aldehídos
aldehídos volátiles
volátiles se
se mantuvo
mantuvo cercana
cercana alallímite
límiteinferior
inferior de
deespecificación,
especificación,
no
hubo crecimiento
no hubo
microbiano
crecimiento
paramicrobiano
ninguno depara
los lotes
ninguno
estudiados
de los lotes
y el comportamiento
estudiados y el en los diferentes
comportamiento
envases
es similar.
Conclusiones:
El producto
es similar.
en condiciones
Conclusiones:
de Temperatura
El producto en
decondiciones
(25 ±2)0C yde60% de
en los
diferentes envases
Humedad
mantiene
sus especificaciones
de calidad
hasta
tres meses dede
elaborado
en las
Temperatura
de (25 ±2)0C
y 60% de Humedad
mantiene
suslos
especificaciones
calidad hasta
losnuevas
tres
condiciones de producción.
Key words: oleozon, estabilidad acelerada
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-035
”Medicinal Plants” Interactive Guide. Guía interactiva "Plantas Medicinales"
Melgarejo López, Nelson; Álvarez Bustamante, Guadalupe; Alonso Abad, Antonio
Facultad de Ciencias Médicas "Dr. Salvador Allende", Cuba
[email protected]
edición
digital
GuíaInteractiva
Interactiva“Plantas
“PlantasMedicinales”
Medicinales”representa
representalalaobra
obrade
dedos
dosmédicos
médicos yyun
un ingeniero
La La
edición
digital
Guía
ingenierocubanos,
agrónomo,
cubanos, en
interesados
en ponerdeal los
servicio
de los profesionales
la salud,
y de los
agrónomo,
interesados
poner al servicio
profesionales
de la salud, de
y de
los lectores
en
lectores
general, unapráctica
herramienta
el empleo
racional medicinales.
de las plantas
general,
unaenherramienta
para elpráctica
empleo para
racional
de las plantas
La medicinales.
temática abordada
se presenta con un lenguaje claro y sencillo, al alcance de todas aquellas personas interesadas en los
La temática
se presenta
consalud
un lenguaje
y sencillo,
al alcance
aquellas
beneficios
que abordada
su uso reporta
sobre la
humana.claro
De una
manera
resumidadey todas
didáctica,
se refleja en
personas
interesadas
en los
beneficiossobre
que su
uso reporta
sobre
la salud
humana.
esta
multimedia
información
actualizada
cuarenta
y seis
de las
plantas
medicinales reconocidas por
las autoridades reguladoras de productos naturales y aprobadas por el Programa Nacional para el Desarrollo
una manera resumida
y didáctica,
seyrefleja
en estaGran
multimedia
sobre
y laDe
Generalización
de la Medicina
Natural
Tradicional.
parte deinformación
las plantasactualizada
incluidas, son
conocidas
cuarenta yen
seis
de las plantas
reconocidas
lasdeautoridades
reguladoras
de productos
y empleadas
el mundo
con muymedicinales
buenos resultados
en el por
alivio
ciertas dolencias
y malestares
comunes;
naturales
y aprobadas en
porcualquier
el Programa
parasolo
el Desarrollo
y la Generalización
de la Medicina
pueden
ser encontradas
latitudNacional
del planeta,
con la distinción
de que en muchas
localidades
Natural el
y Tradicional.
y países,
nombre común cambia por la influencia de culturas y costumbres, no obstante mantienen sin
variación su nombre científico; tampoco sufren modificación significativa los principios activos y componentes
parte de por
las plantas
sonsuconocidas
y empleadas en
en este
el mundo
con se
muy
buenosademás
resultados
de Gran
las mismas,
lo cual incluidas,
se conserva
carácter terapéutico;
material
reflejan
los
en el alivio
de por
ciertas
dolencias
y malestares
comunes;
pueden
ser encontradas
en cualquier su
latitud
delde
principales
usos
aparatos
y sistemas,
las formas
naturales
o farmacéuticas
de presentación,
modo
planeta,
solo con la distinción
de que en
y países,
el su
nombre
comúnycambia
por la
empleo,
la dosificación,
las precauciones
quemuchas
deben localidades
tenerse en cuenta
para
elaboración
administración;
influencia
de culturas
y costumbres,
no además,
obstante de
mantienen
sin variación
su nombre
científico;
tampocode
entre
otros aspectos
de interés.
Dispone
una galería
de imágenes
que refleja
las plantas
sufrenunmodificación
significativa
los principios
componentes
de las mismas,
lo cual se
interés,
índice de formulas
farmacéuticas
y unactivos
breve yglosario
para facilitarle
al lector por
no especializado
la
conserva sudecarácter
este como
material
se reflejan
los principales
aparatos
comprensión
algunosterapéutico;
términos. en
Surge
resultado
de además
la necesidad
de disponer
materialy
usosde
porun
sistemas,
naturales
o farmacéuticas
de presentación,
su modo
empleo, la dosificación,
las
integrador
enlaslaformas
docencia
de pregrado
y postgrado
para la formación
de de
profesionales
de las ciencias
precauciones
que deben
tenerse
en cuenta
elaboración
administración;
entre otros
médicas
en la Atención
Primaria
de Salud
ya quepara
los su
existentes
hastay el
momento en nuestro
país,aspectos
muestran
interés. de una forma muy dispersa y fraccionada. Hasta la actualidad se han realizado diversos
losde
contenidos
Dispone
además,endeeluna
galería
demedicina
imágenesnatural
que refleja
las plantas
de interés, relacionados
un índice de formulas
formulas
estudios
científicos
ámbito
de la
y tradicional
especialmente
con
plantas
farmacéuticas
y un brevenoglosario
parade
facilitarle
al lector
lectorque
no especializado
especializado
la comprensión
comprensión
de algunos
algunos
medicinales,
sin embargo,
se dispone
una publicación
oriente adecuadamente
su manejo,
objetivo
para
facilitarle
al
no
la
de
quetérminos.
nos trazamos con nuestra guía encaminada a dotar a profesionales y promotores de salud, de un
instrumento práctico y eficiente en su capacitación.
Surge como resultado de la necesidad de
de disponer
disponer de
de un
un material
material integrador
integrador en
en la
la docencia
docencia de
de pregrado
pregrado
y postgrado para la formación de profesionales de las
las ciencias
ciencias médicas
médicas en
en la
la Atención
Atención Primaria
Primaria de
de Salud
Salud
ya
que
los
existentes
hasta
el
momento
en
nuestro
país,
muestran
los
contenidos
de
una
forma
muy
Key words: Medicinal Plants, Interactive Guide, Guía interactiva, Plantas Medicinales
dispersa y fraccionada.
Hasta la actualidad se han realizado diversos estudios científicos en el ámbito de la medicina natural y
tradicional especialmente relacionados con plantas medicinales, sin embargo, no se dispone de una
publicación que oriente adecuadamente su manejo, objetivo que nos trazamos con nuestra guía
encaminada a dotar a profesionales y promotores de salud, de un instrumento práctico y eficiente en su
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-036 Evaluation of the anti-inflammatory activity of Sedum praealtum (always live) coming
from the municipality of San Martín de las pirámides, state of Mexico
Berdeja Martínez, Blanca Margarita; Vargas Díaz, M. Elena; Santacruz Ramírez, Ariadna; Rivero
Ramírez, Nora Lilia; Ávila Uribe, Margarita Micaela
Instituto Politécnico Nacional, México
[email protected]
Introduction: the
the main
main objective
objective ofofthe
theinflammatory
inflammatoryreaction
reactionis is
delete
delete
or or
dilute
dilute
thethe
injurious
injurious
agent
agent
withwith
a a
series of humoral and cellular events that preferably
preferably heal
heal and
and rebuild
rebuild to
to the
the injured
injured tissue
tissue(Howard,
(Howard, 1996).
1996).
Most commonly used
used nonsteroidal
nonsteroidal anti-inflammatory
anti-inflammatory agents
agentsare
arethe
thesteroids
steroidsand
andNSAIDs,
NSAIDs,both
both
with
with
very
very
serious adverse effects
effects (Rang,
(Rang, 2008)
2008)objective:
objective:totoevaluate
evaluatethe
theanti-inflammatory
anti-inflammatoryactivity
activity
of of
thethe
aqueous
aqueous
extract of Sedum
Sedum praealtum
praealtum Methodology: extract is prepared by infusion, took dry sprinkler, 6 groups of
rats
Methodology:
each with 6extract
were distributed
is preparedasby(1)
infusion,
witnesstook
without
dry sprinkler,
trading), (3)
6 groups
treated of
with
rats(50(,
each
200
with
and
6 were
400 mg/kg of
the
extract),as(5)(1)with
diclofenac
mg/kg), (3)
(6) treated
positivewith
control,
inflammation
was of
induced
by granuloma
distributed
witness
without(4trading),
(50(,an
200
and 400 mg/kg
the extract),
(5)
with the
and
diclofenac
Administration
(4 mg/kg),
was(6)orally
positive
for seven
control,
days,
an inflammation
at the end iswas
dissected
inducedgranulomas,
by granuloma
stomach
and the
and blood
Administration
samples.
Results:
wasphytochemical:
orally for sevenflavonoids,
days, at the
alkaloids,
end is dissected
saponins granulomas,
and tannins; stomach
pharmacological,
and bloodyou notice
samples.
an
anti-inflammatory
Results: phytochemical:
process dose-dependent
flavonoids, alkaloids,
to exist significant
saponins and
differences
tannins; with
pharmacological,
the positive control
you and
notice
with
diclofenac,
an anti-inflammatory
the histologically
process
showed
dose-dependent
a more severe
to exist
irritation
significant
of the mucosa
differences
in stomach
with thewith
positive
diclofenac,
controland
Melo
andcols
withisolated
diclofenac,
four flavonoids
the histologically
with anti-inflammatory
showed a more activity
severe of
irritation
this plant,
of the
which
mucosa
trapsin radicals
stomachand
with diclofenac,
antioxidants
areMelo
considered
and colsfree
isolated
(Marcano
four flavonoids
et al., 2002).
with The
anti-inflammatory
aqueous extract
activity
of Sedum
of this plant,
praealtum
whichDC,
traps
radicals
and antioxidants
are considered free (Marcano et al., 2002). The aqueous extract of Sedum
presented
anti-inflammatory
effect.
praealtum DC, presented anti-inflammatory effect
Key words: anti-inflammatory activity, Sedum praealtum, always live, San Martín de las pirámides,
México
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-037 Evaluation of the antibabesic activity of three plant species in mice infected with
Babesia microti
Hernández de Jesús, María de Lourdes; Aguilar Figueroa, Blanca Rosa; Oble Hernández, Abraham
Instituto Politécnico Nacional, México
[email protected]
Human babesiosis isis an
an emerging
emerging zoonotic
zoonoticdisease
diseasecaused
causedbybyBabesia
Babesiaspp.
spp.
Haveimportance
importanceto to
public
Have
public
health and veterinary since it has economic impact by affecting the production of cattle. In Central America
America
and
México,
and human
Mexico,cases
human
have
cases
been
have
confirmed
been confirmed
as in South
as inAmerica
South America
and Brazil.
andThe
Brazil.
infection
The infection
of humans
of
humans
and
animals
and occurs
animalsthrough
occurs the
through
bite of
the
infected
bite of ticks
infected
inoculated
ticks inoculated
sporozoites
sporozoites
that become
that trophozoites
become
and
trophozoites the
reproduce,
and erythrocyte
reproduce, the
rupture,
erythrocyte
releasing
rupture,
merozoites
releasing merozoites
that invadethat
other
invade
erythrocytes,
other erythrocytes,
another
another mechanism
mechanism
of transmission
of transmission
is through
is through
transfusion.
transfusion.
Treatment regimes, depending on the degree of B.
microti infection is used: azithromycin with clindamycin and atovaquone with quinine. These drugs cause in
Treatment
patient
sideregimes,
effects so
depending
do not complete
on the degree
their treatment,
of B. microti
resistant
infection
strains
is used:
causing.
azithromycin
An alternative
with is to search
clindamycin
for
plants with
andantiparasitic
atovaquone activity.
with quinine.
In this
These
paper
drugs
the cause
antiparasitic
in patient
activity
side effects
of the so
methanol
do not complete
extracts of
their treatment,
Ibervillea
sonorae,
resistant
Krameria
strains
sonorae
causing.
and An
Phoradendrom
alternative is californicum
to search forinplants
mice infected
with antiparasitic
with Babesia
activity.
microti
In
was
evaluated.
Parasitemiaactivity
curve was
determined
the effect
of the extracts
evaluated
for 33and
days.
this paper
the antiparasitic
of the
methanol and
extracts
of Ibervillea
sonorae,was
Krameria
sonorae
The
results werecalifornicum
subjected in
to mice
statistical
analysis
and Zigma
Zigma
Plot. Observed that two extracts
Phoradendrom
infected
with Babesia
microti
wasStat
evaluated.
showed activity in different degree. So we can conclude that plants are a potential source of chemical
Parasitemiawhich
structures,
curvecould
was determined
be an alternative
and the
in controlling
effect of theparasites.
extracts was evaluated for 33 days. The results
were subjected to statistical analysis and Zigma Zigma Stat Plot. Observed that two extracts showed
activity in different degree. So we can conclude that plants are a potential source of chemical structures,
Key words: antiparasitic activity , babesia microti , plant species
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-038 Determination of adsorption capacity of textile dyes by Peanut shell (Arachis
hypogaea) and parchment coffee (Coffea arabica)
Hernández de Jesús, María de Lourdes; Barragán Huerta, Blanca Estela; Flores Vargas, Eliud
Fernando
Instituto Politécnico Nacional, México
[email protected]
The textile industry is
is one
one of
of the
theactivities
activities that
thatgenerate
generatemore
morepollution
pollutiondue
duetotothe
thelarge
largevolumes
volumesofofwater
water
used in their different processes. Among the
the contaminants
contaminants in
in effluents
effluents are
are textile
textiledyes,
dyes,which
whichdo
donot
notexhibit
high
to living
organisms,
however,
the the
strong
coloring
cometotoabolish
abolishthethe
exhibittoxicity
high toxicity
to living
organisms,
however,
strong
coloringthat
thatthem
them may come
photosynthetic process.
process. The
The adsorption
adsorptionbybyactivated
activatedcarbon
carbonis is
one
of the
most
used
processes
in the
one
of the
most
used
processes
in the
purification of textile effluents;
effluents; however
however the
the high
high cost
costofofthis
thismaterial
materialhas
hasled
ledtotoresearch
researchinto
intonew
newadsorbent
materials,
including agro-industrial
waste. Inwaste.
this work,
peanut
shell and
coffeecoffee
were
adsorbent materials,
including agro-industrial
In thisthe
work,
the peanut
shellparchment
and parchment
were characterized
characterized
as adsorbents
as adsorbents
for Orange
for Orange
II (NII),
II (NII),
AcidAcid
BlueBlue
113 113
(AA(AA
113)113)
andand
Basic
Basic
Green
Green
4 (VB4).
4 (VB4).
The
The wastes
wastes
werewere
ground,
ground,
sieved
sieved
and subjected
and subjected
to different
to different
pre-treatments
pre-treatments
(acidic,
(acidic,
basicbasic
and and
microwave),
microwave),
and
and then
then
the kinetic
the kinetic
adsorption
adsorption
studies
studies
werewere
performed.
performed.
Of the
Of the
three
three
dyesdyes
tested,
tested,
onlyonly
AA AA
113113
andand
VB4VB4
were
were adsorbed
adsorbed
by both
by materials
both materials
duringduring
the first
the 6first
hours
6 hours
of treatment.
of treatment.
Optimization
Optimization
of dye
of dye
adsorption
adsorption
process
process
was
conducted
was conducted
using a central
using acomposite
central composite
design by
design
analyzing
by analyzing
the effecttheofeffect
exposure
of exposure
time (2 and
time10
(2 and
min)
10 min)
and
power
and(10
power
to 50%)
(10 to
of 50%)
the microwaves
of the microwaves
pretreatment
pretreatment
on the adsorption
on the adsorption
capacity.capacity.
It was determined
It was
that
the
removal that
of dye
the residue
whenincreased
the powerwhen
and the
the power
microwave
exposure
time had the
determined
the by
removal
of dye increased
by the residue
and the
microwave
higher value.
Key words: adsorption, waste, coffee, peanuts, textile dyes, microwave
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-039 In Vitro Activity of some fractions from exctract of Ruta graveolens against
Trypanosoma cruzi NINOA strain
Hernández de Jesús, María de Lourdes; Ambriz Hernández, Ana Lizbeth; Aguilar Figueroa, Blanca
Rosa; Barragán Huerta, Blanca Estela
Instituto Politécnico Nacional, México
[email protected]
Trypanosoma.cruzi causes Chagas disease. It is estimated that
that 11–18
11–18 million
million people
people in
in South
South America
America are
infected
by this
disease
and and
100,000
people
are at
risk
contracting
it each
year. year.
The chemotherapy
used
are infected
by this
disease
100,000
people
are
at of
risk
of contracting
it each
The chemotherapy
to
treat
has serious
side effects
and its
efficacy
on theonchronic
phasephase
of disease
is stillisa still
matter
used
to this
treatillness
this illness
has serious
side effects
and
its efficacy
the chronic
of disease
a
of
debate.
Previous studies have shown trypanocidal effect of Ruta graveolens. The objective of this work
matter
of debate.
was
to find
the have
activeshown
fractions,
against effect
T. cruzi
straingraveolens.
NINOA The
in vitro
evaluation,
after was
making
Previous
studies
trypanocidal
of Ruta
objective
of this work
to a
chromatographic
separation
chloroform
extract.
Bioassay-guided
phytochemical
study of the
find the active fractions,
againstof T.the
cruzi
strain NINOA
in vitro
evaluation, after
making a
chromatographic
chloroform
extractseparation
of R. graveolens
of the chloroform
was carriedextract.
out using as toxicity bioassay Artemia salina. The in vitro
Bioassay-guided
trypanocidal
assays
phytochemical
were carried study
out according
of the chloroform
to the protocol
extract
reported
of R. graveolens
elsewhere,was
withcarried
slight modifications.
out using as
toxicity bioassay
Bloodstream
trypomastigotes
Artemia salina.
were
Thequantified
in vitro trypanocidal
by the method
assays
of were
Pizzi.carried
The chloroform
out according
extract
to the
showed a
protocol
67%
mortality
reported
Artemia
elsewhere,
salina,with
wasslight
separated
modifications.
by column
Bloodstream
chromatography.
trypomastigotes
Fractionswere
232,quantified
234, 306,by311,
the method
328
and 397ofwhich
Pizzi.presented the lowest LC50 for biotoxicity test were selected. Fractions 306 and 328 for
The
a
second
chloroform
chromatographic
extract showed
separation
a 67% mortality
were chosen,
Artemiaobtaining
salina, was
fractions
separated
292-318
by column
and 340-390 which
chromatography.
presented
an LC50
Fractions
less. Of232,
fractions
234, 306,
tested
311,
against
328 and
Trypanosoma
397 which presented
cruzi was the
found
lowest
that LC50
the fraction
for
from
biotoxicity
340
to 390 test
at awere
concentration
selected. of
Fractions
100 ug 306
/ ml showed
and 328 higher
for a second
percentchromatographic
lysis against Nifurtimox
separation
reference
were drug
chosen, obtaining
292-318
and 340-390 which presented an LC50 less. Of fractions tested
concentrations
50,fractions
25 and 100
ug / mL.
against Trypanosoma cruzi was found that the fraction from 340 to 390 at a concentration of 100 ug / ml
Key words: trypanosoma cruzi, trypanocidal activity, ruta graveolens
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-040 Evaluation of the in vitro effect of plant extracts of Ruta graveolens and Euphorbia
serpyllifolia on Trypanosoma cruzi strains NINOA and INC-5
Hernández de Jesús, María de Lourdes; Montes de Oca, David de la Rosa; Aguilar Figueroa, Blanca
Rosa; Barragán Huerta, Blanca Estela
Instituto Politécnico Nacional, México
[email protected]
Chagas disease
diseasecaused
caused
by protozoan
the protozoan
Trypanosoma
is life-threatening,
treatment with
by the
Trypanosoma
cruzi is cruzi
life-threatening,
treatment with
Benznidazole and Nifurtimox base ensures
ensures no
no cure
cure for
for the
the disease,
disease,causing
causingside
sideeffects
effectscausing
causingpatients
patients
to discontinue treatment. In this
this work,
work, in
in vitro
vitro trypanocidal
trypanocidal activity
activity of
ofmethanol
methanol extracts
extractsofofRuta
Rutagraveolens
graveolens
and Euphorbia serpyllifolia on Trypanosoma
Trypanosoma cruzi
cruzi strains
strains Ninoa
Ninoa and
and INC
INC -- 55 was
was determined.
determined. The
The strains
strains
were maintained
maintained bybyserial
serial
passage
in CD1
mice, inoculated
intraperitoneally
with blood.
infected blood.
passage
in CD1
mice, inoculated
intraperitoneally
with infected
Determining the trypanocidal effect in vitro was performed by placing blood trypomastigotes with plant
Determining
extracts
and the
reference
trypanocidal
drugs effect
to a final
in vitro
concentration
was performed
of 10,
by 25,
placing
50 and
blood
100trypomastigotes
mg / mL. Percent
with lysis
plant was
extracts andby
determined
reference
the method
drugsoftoPizzi,
a final parasite
concentration
numbers
of 10,
that
25,survived
50 and 100
the mg
treatment,
/ mL. Percent
comparing
lysis with
was the
determined
negative
control.
by theWith
method
Ninoaof strain
Pizzi, showed
parasite Ruta
numbers
graveolens
that survived
a high the
percentage
treatment,ofcomparing
lysis, whilewith
Euphorbia
the
negative control.
serpyllifolia
had no effect, Benznidazole obtained a percentage of 55,25,20 and 5 %, Nifurtimox 47,29,10
and 3%. The INC- 5 strain, Ruta graveolens obtained a low percentage of lysis, while Euphorbia serpyllifolia
With Ninoa
showed
little
strain
activity,
showed
Benznidazole
Ruta graveolens
was 28,a 18,
high13
percentage
and 2 %,ofNifurtimox
lysis, while
was
Euphorbia
Euphorbia
81, 72, serpyllifolia
62 and 52%.
had
had
The
no effect, Benznidazole
methanol
extract Ruta graveolens
percentage of
ofhas
55,25,20
greaterand
and
activity
%,than
Nifurtimox
Benznidazole
47,29,10
and
and
Nifurtimox
3%.The
The on
obtained a trypanocidal
percentage
55,25,20
55%,
Nifurtimox
47,29,10
and
3%.
INC5 strain, Ruta
a low percentage
of lysis, while Euphorbia serpyllifolia showed
Trypanosoma
cruzi graveolens
strain Ninoaobtained
concentrations
tested.
little activity, Benznidazole was 28, 18, 13 and 2 %, Nifurtimox was 81, 72, 62 and 52%.
The methanol extract Ruta graveolens trypanocidal has greater activity than Benznidazole and Nifurtimox
Key words: ruta graveolens, euphorbia serpyllifolia, trypanosoma cruzi
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-041 Nutraceutical quality components of microencapsulated noni juice (Morinda citrifolia
L.) by spray drying
Corrales García, Joel E.; Gallegos García, Flor Adriana; García Mateos, Ma. Rosario; Hernández
Montes, Arturo; Ybarra Moncada, Ma. Carmen
Universidad Autónoma de Chapingo, México
[email protected]
Noni isisa afruit
fruit
with
with
nutraceutical
nutraceutical
potential,
potential,
but its
butunpleasant
its unpleasant
odor and
odor
flavor
andlimit
flavor
its consumption.
limit its consumption.
Encapsulationis the process
process to
to entrap
entrap active
activeagents
agentsinto
intoparticles;
particles;it itis isa apromissory
promissory
technology
technologythat
that
may
may
allow mask bad taste and
and bad
bad smellcomponents.
smellcomponents. The
The aim
aim was
was to
toassess
assess the
the nutraceutical
nutraceutical potential
potential innoni
innoni
juicemicroencapsulated by spray
spray drying.
drying. The
The drying
dryingtemperatures
temperatureswere180
were180and
and8585°C°Cof of
inlet
and
outlet,
inlet
and
outlet,
respectively.Thecarrier materials
materials (maltodextrine
(maltodextrineDEDE-10,10,
arabic
gum,
soy lecithin)
were mixed
in
arabic
gum,
and and
soy lecithin)
were mixed
in
different proportions,
proportions,searching
searchingmaximum
maximum
bad-taste
and bad-smell
masking
and minimum
active
bad-taste
and bad-smell
masking
and minimum
active
compounds alteration. Noni juice was obtained from ripe, cleaned, and scaldedfruits(92 °C 55 min).
min). A pulppulpremoving machine was used and then
then the
the juice
juice was
was obtained
obtained by
by centrifugation
centrifugation of
ofthe
thepulp
pulp(11000
(11000G,
G,55°C
°C
30 min). Phenols,
Phenols, flavonoids,
flavonoids, vitamin
vitamin CC and
and antioxidant
antioxidantactivity
activity(by
(bythe
theDPPH
DPPHinhibition
inhibitiontechnique),
technique),drying
drying
efficiency and residual humiditywere measured. There was some important alteration of active compounds
in different
differentlevels
levels
(upon
(upon
the compound)by
the compound)by
high temperatures,
high temperatures,
however, however,
the best treatment
the best
of treatment of
microencapsulated noni
noni juice
juicewas
waswith
with
7070
% maltodextrin
% maltodextrin
+ 29.9
+ 29.9
% arabic
% arabic
gum +
gum
0.1 %
+ 0.1
soy %
lecithin
soy lecithin
(treatment 3)
3) totokeep
keepthe
themajor
majorquantity
quantity
of of
total
total
phenols,
phenols,
antioxidant
antioxidant
activity,
activity,
this treatment
this treatment
showedthe
showedthe
highest drying
drying efficiency
efficiency and
andcaused
causedthe
the
lowest
lowest
residual
residual
humidity.
humidity.
A high
A high
positive
positive
correlation
correlation
between
between
phenols and antioxidant activity was observed. The sensory
sensory evaluation
evaluation showed
showed that
that the
the microencapsulated
microencapsulated
noni
juice with treatment
noni juice
3 waswith
significantly
treatmentless
3 was
rejected
significantly
(22 %)less
thanrejected
the natural
(22 juice
%) than
(46the
%).natural juice
Key words: phytochemicals, microencapsulated, spray drying, antioxidant activity, sensory evaluation
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-042 Phytochemical content of noni juice (Morinda citrifolia) microencapsulated in W/O/W
emulsions
Rodríguez Barahona, Carmen María; Corrales García, Joel E.; Hernández Montes, Arturo; Ybarra
Moncada, Ma. Carmen
Universidad Autónoma de Chapingo, México
[email protected]
Noni is a bush
ripen
have
unpleasant
and
penetrating
bush of
ofAsiatic
Asiaticorigin
originwhich
whichproduces
producesfruits
fruitsthat
thatwhen
whenget
get
ripen
haveanan
unpleasant
and
smell.
The noni
hasThe
a lotnoni
popularity
because
of itsbecause
nutraceutical
a healing properties,
however,
its unpleasant
penetrating
smell.
has a lot
popularity
of its nutraceutical
a healing
properties,
smell
disfavors
its consumption.
The microencapsulation
multiple
emulsions is an
for masking
however,
its unpleasant
smell disfavors
its consumption. by
The
microencapsulation
byalternative
multiple emulsions
is
smells
and minimize
the smells
factorsandthat
interfere
secondary
metabolites
stability.
The nutraceutical
an alternative
for masking
minimize
the in
factors
that interfere
in secondary
metabolites
compounds
noni juice compounds
are sensibleoftononi
highjuice
temperatures,
whichtemperatures,
the objectiveforofwhich
this study
stability. The of
nutraceutical
are sensiblefor
to high
the is to
microencapsulate
noniisjuice
by multiple emulsions
disregarding
theemulsions
use of heat.
To achieve the
thisuse
procedure
objective of this study
to microencapsulate
noni juice
by multiple
disregarding
of
heat. juice
noni
To achieve
was trapped
this procedure
in multiple
noni
emulsions,
juice was using
trapped
as inwall
multiple
material
emulsions,
soy protein
usingisolate
as wall
(SPI)
material
and Arabic
soy
protein(GA).
gum
isolate
Phenolic,
(SPI) andflavonoid
Arabic gum
and(GA).
ascorbic
Phenolic,
acid flavonoid
content and
of three
ascorbic
emulsions
acid content
W/O/W
of three
with polymer
emulsions W/O/W
concentration
(SPI-GA)
with polymer
of 6, 7.5concentration
and 9 % were(SPI-GA)
measured.
of 6,
The
7.5results
and 9 showed
% were measured.
that did not The
existresults
significant
showed that
difference
(Pdid
< 0.05)
not exist
in phenolic
significantand
difference
ascorbic(Pacid
< 0.05)
content
in phenolic
among and
treatments.
ascorbic The
acid levels
contentofamong
flavonoids
treatments.
where
higherThe
in the
levels
treatments
of flavonoids
than the
where
oneshigher
that existed
in the treatments
in the noni juice
than control.
the onesAs
that
conclusion
existed inthe
thepolymer
noni
juice control.
content
in theAs
multiple
conclusion
emulsions
the polymer
did not content
interfered
in in
thethe
multiple
phytochemical
emulsionscontent
did notamong
interfered
treatments.
in the
Key words: multiple emulsions, secondary metabolite, nutraceutical, noni juice
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-043
Antifungal activity of the root of Adenophyllum aurantium (L.) Strother
Calderón Oropeza, Mónica Alicia; Sánchez Espinosa, Ana Claudia; Hernández Carlos, Beatriz; Cruz
Vázquez, Julieta Karina; Cruz Cerón, Nallely Anali; Rojas Orizonte, Miriam
Universidad del Mar, México
[email protected]
Medicinal plants
plants research
researchhas
has
made
made
available
available
substances
substances
for the
fortreatment
the treatment
of differenthuman
of differenthuman
and
and
agricultural pests.
pests. The
The natural
naturalproducts
productsrepresent
represent
an an
alternative
alternative
to synthetic
to synthetic
antifungal.
antifungal.
México
México
is theis the
country with more species of Asteraceaein the
the world, accounting
accounting for
for 12-15
12-15 %
% of
of México's
México's vascular
vascular flora.
flora. ItIt
has been reportedthat the
the Asteraceae
Asteraceae family
family isisan
animportant
importantsource
sourceofofpesticides.Specie
pesticides.Speciethat
that
belongs
belongsto to
this family is Adenophyllumaurantium(L.) Strotherknown
Strotherknown as
as 'rudamontes'
'rudamontes' endemic
endemic of
ofMéxico.
México.The
Thepresenc e
presence
of
bioactive
of bioactive
compounds
compounds
has been has
demonstrated
been demonstrated
recently against
recentlyroot
against
nematode
root nematode
Nacobbusaberrans and
Nacobbusaberrans
fungal
pathogens Fusariumsolaniand
and fungal pathogens
Alternariaalternata.
Fusariumsolaniand
Hexane
Alternariaalternata.
and the ethyl acetate
Hexane
extract
and of
theroot
ethyl
were
acetate extract
prepared
to determine
of root were
inhibition
prepared
of growth
to determine
mycelialin
inhibition
Fusariumoxysporum
of growth mycelialin
and Rhizoctoniasolani;
Fusariumoxysporum
the agar
and Rhizoctoniasolani;
diffusion
assay, Petri dishes
the agar
with
diffusion
potato assay,
dextrose
Petri
agar,
dishes
thiabendazole
with potato dextrose
(+) and ethanol
agar, thiabendazole
(-) controls was
(+)
and ethanol
carried
out. (-)
Mycelial
controls
growth
was carried
rate of out.
the Mycelial
second, growth
third and
ratefourth
of theday
second,
of incubation
third and was
fourth
determined.Lik
day of
e
preliminary
result
was found thatpreliminary
fungi were result
sensitive
extracts,
and it were
wouldsensitive
be interesting
to identify
incubation was
determined.Like
wastofound
that fungi
to extracts,
and itthe
substance responsible for this activity.
Key words: adenophyllumaurantium, antifungal activity, growth rate
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-044
Characterization of policosanol from wax cochineal
Martínez Ayala, Alma Leticia; Ramos Z., Emilia; Jiménez Aparicio, Antonio R.
Centro de Investigación en Biotecnología Aplicada, Tlaxcala, México
[email protected]
Wax cochineal
cochineal isis an
anagrowaste
agrowaste product
productwhich
whichcontains
containsconsiderable
considerableamount
amount
of of
coccerina.
coccerina.
ThisThis
hashas
a a
good potential for obtaining
obtaining policosanol.
policosanol. The
The objetive
objetive ofofthis
thisstudy
studywas
wastotoexamine
examinethe
thealiphatic
aliphatic
alcohols
alcohols
content of
of wax
wax cochineal
cochineal(Dactylopiuscoccus)
(Dactylopiuscoccus)extracts
extracts
from
from
nymphs
nymphs
andand
adultadult
stages.
stages.
Extracts
Extracts
were were
obtained by applying alkaline hydrolysis reaction.
reaction. All
All the
the obtained
obtained extracts
extracts were
were characterized
characterized chemically
chemically
by Fourier
Fourier Transform
Transform Infrared
Infrared Spectroscopy
Spectroscopy(FTIR)
(FTIR)in in
thethe
attenuated
attenuated
total
total
reflectance
reflectance
(ATR)
(ATR)
sampling
sampling
mode. For
Forother
otherside,
side,
thethe
aliphatic
aliphatic
alcohols
alcohols
werewere
identified
identified
and and
quantified
quantified
by Gas
by Chromatography
Gas Chromatography
coupled to
to Mass
Mass Spectrometric
Spectrometric techniques
techniques (GC-MS).
(GC-MS). ATR-FTIR analysis indicate presence of aliphatic
ATR-FTIRin analysis
alcohols
all the extracts,
indicatewhich
presence
was of
confirmed
aliphatic by
alcohols
GC- MS.
in all
The
thecomposition
extracts, which
of aliphatic
was confirmed
alcoholsbyofGCthe
waxy
extracts
derived offrom
cochineal
wasofvery
similar,
being derived
1-triacontanol
the most was
abundant
alcohol.
MS. The
composition
aliphatic
alcohols
the waxy
extracts
from cochineal
very similar,
The
content of
was
found The
by the
method
1 (5.24±0.36
g/kg), followed
of method
beinghighest
1-triacontanol
the1-triacontanol
most abundant
alcohol.
highest
content
of 1-triacontanol
was found
by the 2
(1.575±0.43
g/kg) y method
3 (0.520±0.03
g/kg)
of wax cochineal
adult
stage. In wax
method 1 (5.24±0.36
g/kg), followed
of method
2 (1.575±0.43
g/kg) y from
method
3 (0.520±0.03
g/kg)cochineal
of wax
cochinealfrom
extracts
from
nymphs
adult stage.
stage,Inwas
wax
found
cochineal
likewise
extracts
by method
from 1nymphs
the higuest
stage,
content
was found
(1.66±0.41
likewise
g/kg),
by method
followed
1 the
of
method
higuest
2 content
(0.515±0.05
(1.66±0.41
g/kg) yg/kg),
method
followed
3 (0.173±0.07
of method g/kg)
2 (0.515±0.05
(p<0.05). g/kg)
Highy yield
method
of triacontanol
3 (0.173±0.07
was
g/kg) (p<0.05).
recuperated
of cochineal wax in both insect stages. This component is important in the farmaceutical
High
yield
of in
triacontanol
was recuperated
cochineal
waxcould
in both
stages.source
This component
is
industry
and
the agriculture,
by the whichofwax
cochineal
be insect
an important
of triacontanol.
important in the farmaceutical industry and in the agriculture, by the which wax cochineal could be an
Key words: policosanol, triacontanol, cochineal, wax, insect
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-045
Use of Citrus lemon L. (lemon) in the treatment of rises of arterial pressure
Avello Lorca, Marcia Andrea ;Jofré, P.; Fernández Pastene, E.
University of Concepción, Chile
[email protected]
Introducción. La
Lahipertensión
hipertensiónarterial
arterial
es es
una una
enfermedad
enfermedad
crónica,
crónica,
de etiología
de etiología
multifactorial
multifactorial
y que y que
corresponde aa lalaelevación
elevaciónpersistente
persistentedede
la presión
la presión
arterial
arterial
sobre
sobre
los límites
los límites
referenciales.
referenciales.
EntreEntre
los los
problemas de salud
salud aa los
los que
que puede
puede verse
verseenfrentado
enfrentado elelpaciente
pacientehipertenso,
hipertenso,destacan
destacanlas
lasalzas
alzasdede
presión
presión arterial.
arterial.
Aquí, el Aquí,
paciente
el paciente
puede optar
puedepor
optar
acudir
por aacudir
algúnasistema
algún sistema
públicopúblico
de urgencia
de urgencia
o realizar
o realizar
medidas
medidas
de
autoatención.
de autoatención.
A nivel popular
A nively popular
en diferentes
y en diferentes
fuentes defuentes
información
de información
se menciona
se menciona
que el zumo
quedeelCitrus
zumo de
Citrus limon
L. Burm. f.(Rutaceae)es
útil para
disminuir
la presión
arterial. Sin
embargo,
limon
L. Burm.
f.(Rutaceae)es
útil para disminuir
la presión
arterial.
Sin embargo,
este
uso no este
tiene un
uso no tiene
un respaldo
científico
del todo El
claro.
respaldo
científico
del todo
claro. Objetivo.
objetivo del trabajo fue analizar el uso del zumo de C. limon,
Objetivo.
en
el manejo
El objetivo
del alza
delde
trabajo
presión
fue arterial.
analizar Metodología:Para
el uso del zumo deesto
C. limon,
se analizó
en el la
manejo
composición
del alza química
de presión
de
arterial.de C. limón disponibles en la ciudad de Concepción, Chile, a través de métodos espectroscópicos y
frutos
Metodología:Para Para
cromatográficos.
esto caracterizar
se analizó laelcomposición
uso del zumo,
química
se aplicó
de frutos
una encuesta
de C. limón
a 506
disponibles
pacientes
enhipertensos
la ciudad
de Concepción,
del
CESFAM Dr.Chile,
VíctoraManuel
través de
Fernández
métodos de
espectroscópicos
la ciudad de Concepción,
y cromatográficos.
y se realizó
Parauncaracterizar
estudio piloto
el uso
con
delpacientes
5
zumo, sehipertensos,
aplicó una encuesta
para evaluar
a 506
efecto
pacientes
del zumo
hipertensos
frente undel
alza
CESFAM
de presión
Dr.arterial.
Víctor Manuel
Resultados:Como
Fernández se
resultados
de identificaron
la ciudad de polifenoles
Concepción,presentes
y se realizó
en un
el zumo
estudio
depiloto
C. limon
con como
5 pacientes
los flavonoides
hipertensos,
eriocitrina,
para
evaluar efecto
hesperidina
y diosmina.
del zumo frente
Con respecto
un alza de
a los
presión
datosarterial.
compilados de la encuesta, el 95.2% de la población
Resultados:Como
encuestada
ha usado
resultados
alguna se
vezidentificaron
zumo de C.polifenoles
limon antepresentes
un alza de
enpresión
el zumoarterial,
de C. limon
con una
como
mejoría
los de
flavonoides
los
síntomaseriocitrina,
aproximadamente
hesperidina
en ymedia
diosmina.
hora.Con
Enrespecto
el estudio
a los
piloto
datos
secompilados
observó una
de disminución
la encuesta, de
el la
95.2% de
presión
arterial
la población
en hipertensos
encuestada
en situación
ha usadodealguna
alza devez
presión,
zumo de
queC.tiende
ser sostenida
el tiempo, no
limonaante
un alza deen
presión
arterial,
así
en pacientes
con una mejoría
normotensos.
de los síntomas
Discusiónaproximadamente
y Conclusión. Elen
uso
media
del zumo
hora. En
de el
C.estudio
limon frente
piloto un
se alza de
observó arterial,
presión
una disminución
está ampliamente
de la presión
arraigado
arterialenenlahipertensos
cultura popular
en situación
y se concluyó
de alza
que
dedisminuye
presión, que
la presión
tiende
a ser sostenida
arterial
por un mecanismo
en el tiempo,denoacción
así en no
pacientes
establecido,
normotensos.
probablemente relacionado con el contenido en
Discusión yyConclusión.
El uso del zumo
C. limon
frente
alza de efectos
presión antihipertensivos.
arterial, está ampliamente
polifenoles
vitamina C, compuestos
a losdecuales
se les
hanun
atribuido
arraigado en la cultura popular y se concluyó que disminuye la presión arterial por un mecanismo de
acción no establecido, probablemente relacionado con el contenido en polifenoles y vitamina C,
Key words: Citrus lemon L., treatment, arterial pressure
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-046 Evaluation of the hypoglycemic effect of Bauhinia forficata in patients with poorly
controlled diabetes
Córdova Mariángel, Pía; Avello Lorca, Marcia Andrea; Fernández, P.; Villa, L.
University of Concepción, Chile
[email protected]
Introduction. Diabetes
Diabetes mellitus
mellitusisisa achronic
chronic
disease
become
increasingly
prevalent
in recent
disease
thatthat
has has
become
increasingly
prevalent
in recent
decades. The
The objective
objective of
oftreatment
treatmentisistotoreduce
reducecardiovascular
cardiovascularrisk.
risk.
The
The
useuse
of medicinal
of medicinal
plants
plants
in the
in the
treatment of chronic diseases has
has attracted
attracted scientific
scientific interest
interest ininrecent
recentyears.
years.InInChile,
Chile,a acommon
commonform
formofof
alternative medicine for
for diabetes
diabetes is
is the
theleaf
leafofofthe
theBauhinia
Bauhiniaforficata
forficata("Pata
("PatadedeVaca")
Vaca")plant.
plant. Objective. To
evaluate the hypoglycemic effect of B. forficata leaves, administered as complementary therapy for patients
Objective.
with
poorly To
controlled
evaluatetype-2
the hypoglycemic
diabetes mellitus.
effect Methodology.
of B. forficata leaves,
A semi-experimental
administered study
as complementary
was conducted on
therapy for
Chilean
patients
patients
in awith
primary
poorly
healthcare
controlledsetting
type-2who
diabetes
have poorly
mellitus.
controlled type-2 diabetes mellitus (HbA 1C
>7%). These patients were administered a 0.4% infusion of B. forficata leaves, twice daily for a period of
Methodology.
three
months, Aand
semi-experimental
then a period ofstudy
one month
was conducted
without infusion.
on Chilean
Clinical
patients
parameters
in a primary
such
healthcare
as body mass
setting(BMI),
index
who have
bloodpoorly
pressure
controlled
and blood
type-2
glucose
diabetes
weremellitus
measured
(HbA1C
every>7%).
month.
These
Total patients
cholesterol,
weretriglycerides
administered
and
glycated hemoglobin
a 0.4% infusion
(HbA1C)
of B. forficata
were measured
leaves, twice
every daily
threeformonths.
a periodResults.
of threeFor
months,
patients
and
receiving
then a the
period ofofone
infusion
B. forficata
month without
leaves,infusion.
a decrease
Clinical
in HbA1C
parameters
levelssuch
of 0.5%
as body
wasmass
observed.
index No
(BMI),
significant
blood pressure
changes
and
in
weight,
blood blood
glucose
pressure,
were measured
total cholesterol,
every month.
triglyceride
Total cholesterol,
and glucosetriglycerides
levels were and
observed.
glycated
Discussion
hemoglobin
and
(HbA1C) were
Conclusion.
Themeasured
infusion of
every
B.forficata
three months.
leaves, when used as an adjunct to pharmacological treatment along
with a healthy lifestyle, may decrease the levels of HbA1C in the adult diabetic population.
Results. For patients receiving the infusion of B. forficata leaves, a decrease in HbA1C levels of 0.5%
was observed. No significant changes in weight, blood pressure, total cholesterol, triglyceride and
glucose levels were observed.
Discussion and Conclusion. The infusion of B.forficata leaves, when used as an adjunct to
pharmacological treatment along with a healthy lifestyle, may decrease the levels of HbA1C in the adult
Key words: hypoglycemic effect, Bauhinia forficata, patient, diabetes
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-047 In vitro Anthelmintic Efficacy of Aloe ferox, Agave sisalana and Gunnera perpensa
Against Heterakis gallinarum
Manjoro Mwale, Marizvikuru
Institute for Rural Development, University of Venda, South Africa
[email protected]; [email protected]
Heterakis gallinarum
gallinarum impedes
impedesthethe
productivity
productivity
of village
of village
chickens
chickens
and hence
and their
hence
socio-economic
their socio-economic
contribution to
to rural
rural livelihoods.
livelihoods.Smallholder
Smallholderfarmers
farmersareare
endowed
endowed
withwith
vastvast
indigenous
indigenous
knowledge
knowledge
for for
controlling H. gallinarum and
and they
they predominantly
predominantly use
useAloe
Aloeferox,
ferox,Agave
Agavesisalana
sisalanaand
andGunnera
Gunneraperpensa.
perpensa.
However, their
their anti-helminthic
anti-helminthic efficacy
efficacy isisunknown.
unknown.Therefore,
Therefore,the
the
study
study
objective
objective
waswas
to determine
to determine
the the
anthelmintic efficacy
efficacy ofofA.A.ferox,
ferox,
A. A.
sisalana
sisalana
andand
G. perpensa
G. perpensa
against
against
H. gallinarum
H. gallinarum
in vitro.inTen
vitro.
H. Ten H.
gallinarum worms were randomly introduced
introduced into
into each
each of
of the
the 42
42 petri-dishes.
petri-dishes. InIn triplicate,
triplicate, treatments
treatments were
were
positive control (distilled water), negative control (mebendazole) and A. ferox, A. sisalana and G.
G. perpens a
perpensa leaf
aqueous
aqueous
extracts
leafwith
extracts
doseswith
7.25,
doses
14.5,7.25,
29 and
14.5,
58 29
mg/mL.
and 58
Worm
mg/mL.
motility
Worm
inhibition
motilityand
inhibition
worm and
recovery
wormnoted
was
recovery
over was
60 h.noted
Data over
was 60
analysed
h. Datausing
was analysed
general linear
usingmodel
general
of Statistical
linear model
Analyses
of Statistical
System. Agave
Analyses(14.5
sisalana
System.
mg/mL)
Agave
hadsisalana
highest (14.5
(80%)mg/mL)
worm motility
had highest
inhibition
(80%)
at 12-h
worm
interval
motility
(p<0.05)
inhibition
and
at highest
12-h worm
interval (p<0.05)
mortality
index (80%)
and highest
showingworm
that the
mortality
plant has
index
anthelmintic
(80%) showing
properties.
that the
Worms
plant(70%)
has anthelmintic
were recovered after
48
h following
treatment
ferox (58 after
mg/mL)
50% for
G. perpensa
allferox
dose(58
levels.
Thisand
signifies
properties.
Worms
(70%)with
wereA.recovered
48 hand
following
treatment
with A.
mg/mL)
that
hasall
moderate
anthelmintic
activities
while
those of
A. moderate
ferox are anthelmintic
primarily ascribed
to its
50% G.
for perpensa
G. perpensa
dose levels.
This signifies
that G.
perpensa
has
activities
purgative
effects.
Agave
mg/mL)
was
the most
efficacious
reducing
motility
and was
causing
while those
of A. ferox
aresisalana
primarily(14.5
ascribed
to its
purgative
effects.
Agave insisalana
(14.5
mg/mL)
mortality
H. gallinarum
in vitro. motility
Findings
arecausing
useful to
resource-constrained
selecting
best
the most of
efficacious
in reducing
and
mortality
of H. gallinarumfarmers
in vitro. for
Findings
arethe
useful
to resource-constrained
plant
and pharmaceutics farmers
for manufacturing
for selecting
anthelmintic
the best plant
drugs.
and pharmaceutics for manufacturing
Key words: chickens, heterakis gallinarum, plant leaf extracts, worm mortality, worm motility
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-048
Immune modulatory effect of Korean native chicken meat
Kang Hyun, Leem; Myung Gyou, Kim; Hye Kyung, Kim
Semyung University, South Korea
[email protected]
Introduction: Chicken essence
essence has
has been
beenwell
wellknown
knownforforitsits
health-promoting
health-promotingand
and
enhancement
enhancement
of body
of body
immunity. However, the mechanism underlying
underlying its
its effect
effect remains
remains unclear.
unclear. This
This study
studyaims
aimstotoexamine
examinethe
the
immune-modulatory effect
effect ofoffour
four
different
different
Korean
Korean
nativenative
chickens
chickens
(Yeonsan
(Yeonsan
Ogye, Hyunin
Ogye, Hyunin
Black, Black,
Hwangbong, and Hoengseong Yakdak) on macrophages. White Leghorn was
was used
used as
as aa control.
control. Materials
Materials
and
Methods:
and Methods:
Breast and
Breast
leg meat
and leg
were
meat
grounded
were grounded
and extracted
and extracted
with 70%with
ethanol
70% at
ethanol
60 oCatfor
606oC
hours.
for
6 hours.
The
proliferation
The proliferation
of macrophage
of macrophage
like RAW264.7
like RAW264.7
cells wascells
assayed
was assayed
by a colorimetric
by a colorimetric
immunoassay, which
immunoassay,
is
based on quantitatingbromodeoxyuridine
which is based on quantitatingbromodeoxyuridine
(BrdU) incorporation(BrdU)
into the
incorporation
newlysynthesized
into the DNA. The
newlysynthesized
uptake
of fluorescein-labeled
DNA. The uptake
Escherichia
of fluorescein-labeled
coli was measured
Escherichia
to determine
coli was measured
whether chicken
to determine
extract
whether chicken
stimulates
the phagocytic
extract stimulates
activity ofthe
RAW264.7
phagocytic
cells.
activity
The of
quantity
RAW264.7
of nitric
cells.
oxide
The(NO)
quantity
was of
determined
nitric oxideby
(NO) was
Griess
assay
determined
in LPS-stimulated
by Griess RAW
assay264.7
in LPS-stimulated
cells. Results:
RAW
All chicken
264.7 cells.
strains of breast meat extracts(50Results:
200
ug/mL)
All chicken
increased
strains
cell proliferations
of breast meat
while
extracts(50-200
leg meats fail to
ug/mL)
affect increased
cell proliferations.
cell proliferations
Breast meat
while
extracts
leg
meatsug/mL)
fail to affect
proliferations.
extracts
(200Black
ug/mL)
two strains ofYakdak)
Korean enhanc
native ed
(200
of twocell
strains
of KoreanBreast
native meat
chicken
(Hyunin
andofHoengseong
chicken
the
phagocytic
(Hyuninability.
BlackFurthermore,
and Hoengseong
LPS-Yakdak)
stimulated
enhanced
release the
of nitric
phagocytic
oxide was
ability.
significantly
Furthermore,
decreased
LPS- by
stimulated
breast
meats
release
of fourof Korean
nitric oxide
native
was
chicken
significantly
strainsin
decreased
dose-dependent
by breastmanner
meats of
while
four breast
Koreanmeat
native
of White
chicken strainsin
Leghorn
fail to affect
dose-dependent
nitric oxide release.
manner
Nitric
while
oxide
breast
production
meat of was
White
notLeghorn
affectedfail
by to
legaffect
meats.
nitric
Conclusion:
oxide
release.results
These
Nitric oxide
suggests
production
that Korean
was notnative
affected
chicken
by legmeat,
meats.especially breast meat, stimulates immune
Conclusion:
functions
of macrophages.
These results suggests that Korean native chicken meat, especially breast meat, stimulates
Key words: korean native chicken, raw 264.7 cells,nitric oxide, phagocyte activity, immune function
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-049
Concentrations of Se as an essential metal in human milk of venezolan women
Angulo Mavo, Andrea María; Chávez, Mervin; Granadillo Morán, Víctor Alberto
Facultad Experimental de Ciencias, Venezuela
[email protected]
Human milk is a biological
biological fluid,
fluid, which
whichprovides
provides all
allthe
thenutrients,
nutrients,including
includingessential
essentialmetals
metalsthat
thatare
arerequired
required
for
the development
for the development
and growth
and growth
of newborn
of newborn
children
children
duringduring
the first
the first
months
months
of life;
of life;
also,
also,
it itcontains
contains enzymes,
enzymes,
specific proteins,
specific proteins,
nucleotides,
nucleotides,
etc. Se concentration
etc. Se concentration
in milk is
in directly
milk is directly
affectedaffected
by levels
by in the
levels chain,
food
in the also
food chain,
is a component
also is a component
of two enzymes,
of two enzymes,
glutioneperoxidase
glutioneperoxidase
thats protects
thats protects
against against
oxidative
oxidative damage,
damage,
and of type
and I ofiodothyronine
type I iodothyronine
5-de-iodinase,
5-de-iodinase,
this antioxidative
this antioxidative
cellularcellular
Se might
Se might
have have
a role
a in
role in preventing
preventing
Cancer.Cancer.
As essential
As essential
nutrients,
nutrients,
many chemicals
many chemicals
can be can
transferred
be transferred
from body
fromstorage
body storage
sites and
sitesblood
the
and the
of nursing
blood ofmothers
nursing mothers
to breasttomilk.
breast
Nowadays,
milk. Nowadays,
researchs
researchs
on the importance
on the importance
of essential
of
trace
essential
metals
in trace
human
metals
milk, in
itshuman
nutritional
milk,quality
its nutritional
and its relation
quality and
withitsenvironmental
relation with environmental
factors are growing.
factors Inare
this
growing.
work,
it isInpresented
this work,the
it istotal
presented
concentrations
the total of
concentrations
Se in samplesofinSehuman
in samples
milk using
in human
ICP- milk
MS. using
The samples
ICPMS. The
were
collected
samples
fromwere
breastfeeding
collected from
women
breastfeeding
between 2 women
and 8 post-partum
between 2 and
months
8 post-partum
in the Hospital
months
of Children
in the
of
VeritasofinChildren
Maracaibo
city. For
of organic
material, digestion
the real digestion
samples with
HNO3
Hospital
of Veritas
in destruction
Maracaibo city.
For destruction
of organicofmaterial,
of the
real /
samplesboth
H2O2.,
with concentrated,
HNO3 / H2O2.,
was
both
applied.
concentrated,
The totalwas
metal
applied.
concentration
The totalwas
metal
0,011
concentration
mg L-1 of was
Se. 0,011
The limit
mgdetection
of
L-1 of Se.
(LD=3?/m)
The limit of
obtained
detection
was(LD=3?/m)
0,003 for Se.
obtained
The precision
was 0,003
(expressed
for Se. The
as precision
RDS) was(expressed
< 5%. Accuracy
as
RDS)assessed
was
was < 5%.byAccuracy
recoverywas
studies,
assessed
obtaining
by recovery
mean studies,
recoveries
obtaining
percentages
mean recoveries
of 100 ± 5%.
percentages
Developed
of
100 ± 5%.forDeveloped
methods
the determination
methods of
fortotal
the determination
concentration of total
the studied
concentration
metal was
of the
accuracy,
studied metal
precise
was
and free
from interferences.
Key words: human milk, essential metal, icp-ms
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-050 Chemical composition of Salvia aratocensis extract obtained by supercritical fluid
extraction and its antigenotoxicity against ultraviolet radiationinduced DNA damage
Fuentes Lorenzo, Jorge Luis; Stashenko, Elena E.; Quintero Ruiz, Nathalia
Colombia
[email protected]; [email protected]
Introduction: Salviais
Salviais aacosmopolitan
cosmopolitanand
and
important
important
genus,
genus,
widely
widely
usedused
in flavoring
in flavoring
and traditional
and traditional
The
present
work
evaluated
the the
medicine for
for treatments
treatments ofofrespiratory
respiratoryand
andgastrointestinal
gastrointestinaldiseases.
diseases.
The
present
work
evaluated
chemical composition of Salvia aratocensis extract
extract obtained
obtained by
by CO2
CO2 supercritical
supercritical fluid
fluid extraction,as
extraction,as well
well
as its
itsDNA
DNAprotective
protective
effect
effect
against
against
ultraviolet
ultraviolet
radiation
radiation
(UVR)-induced
(UVR)-induced
genotoxicity.
genotoxicity. Material and
Material andaratocensis
Methods:S.
Methods:S.extract
aratocensis
composition
extract was
composition
determined
wasbydetermined
gas chromatography-mass
by gas chromatography-mass
spectrometry
spectrometry
(GC-MS).
The(GC-MS).
antigenotoxic
The antigenotoxic
effect of S. aratocensis
effect of S. aratocensis
extract was extract
assayed
was
in assayed
co-incubation
in co-incubation
procedures
procedures
using
the SOS
using
chromotest
the SOS in
chromotest
Escherichia
in coli
Escherichia
PQ37 cells.
coliResults
PQ37 cells.
and Discussion: The major compounds
Results
of
the plant
and extract
Discussion:
were The
?-cadinol
major (31.8%),
compounds
1,10-epiof the cubenol
plant extract
(12.9%),
were trans-?-caryophyllene
?-cadinol (31.8%), 1,10-epi(7.7%), cubenol (12.9%),
cadinene
(6.6%), trans-?-caryophyllene
and squalene (6.5%).(7.7%),
S. aratocensis
-cadineneextract
(6.6%),
protected
and squalene
bacterial
(6.5%).
cells S.
against
aratocensis
UVRextract protected
induced
genotoxicity
bacterial
at doses
cellsbetween
against 0.063
UVR-induced
and 1 mg/mL.
genotoxicity
The results
at doses
werebetween
discussed
0.063
in relation
and 1 mg/mL.
to the
The results
DNA
protective
werepotential
discussed
of the
in relation
S. aratocensis
to the DNA
extract
protective
and its potential
major constituents.
of the S. aratocensis extract and its
Key words: salvia aratocensis, supercritical fluid extraction, antigenotoxicity, sos chromotest, ultraviolet
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-051 Lippia origanoides extract obtained by supercritical fluid extraction and its major
constituent pinocembrin diminish ultraviolet radiation-induced DNA damage and
modulate cell division in Escherichia coli
Fuentes L., Jorge Luis; Rey Castellanos, Natalia; Estévez Castro, Carlos Felipe; Stashenko, Elena E.
Colombia
[email protected]; [email protected]
Introduction: Lippia origanoides is
is an
an aromatic
aromatic shrub
shrub native
native totoCentral
Centraland
andSouth
SouthAmerica.
America.The
Theplant
plantisisused
used
as
seasoning
as seasoning
and in
andtraditional
in traditional
medicine
medicine
for for
treatment
treatment
of gastrointestinal,
of gastrointestinal,
genitourinary
genitourinaryand
andrespiratory
respiratoryasdiseases
diseases
well as as
anti-inflammatory
well as anti-inflammatory
and analgesic
and analgesic
remedy.The
remedy.The
present work
present
evaluated
work evaluated
the chemical
the
chemical composition
composition
of L. origanoides
of L. origanoides
extract obtained
extract obtained
by CO2 supercritical
by CO2 supercritical
fluid extraction,
fluid extraction,
and its antigenotoxic
and its
antigenotoxic
activity
againstactivity
UVR-induced
against UVR-induced
genotoxicity. Major
genotoxicity.
constituent
Major
of constituent
this extract,ofpinocembrin,
this extract, was
pinocembrin,
also studied.
was also and
Material
studied.
Methods:L. origanoides extract composition was determined by gas chromatography-mas s
spectrometry (GC-MS). The antigenotoxic effect of L. origanoides extract was assayed in co-incubation
Material andusing
procedures
Methods:L.
the SOS
origanoides
chromotest
extract
in Escherichia
composition
coliwas
PQ37
determined
cells. Fluorescence
by gas chromatography-mass
microscopy analysis
spectrometry
was
used in cell
(GC-MS).
treatments
The in
antigenotoxic
order to determine
effect ofinterferences
L. origanoideswith
extract
cell division;
was assayed
different
in co-incubation
nucleoid shapes
procedures were
categories
usingmeasured.
the SOS chromotest
Results and
in Escherichia
Discussion: coli
ThePQ37
principal
cells.
constituents
Fluorescence
of the
microscopy
plant extract
analysis
were
was used in cell
pinocembrin
(54.9%),
treatments
trans-?in order
caryophyllene
to determine
(10.9%),
interferences
carvacrolwith
(6.3%),
cell division;
p-cimene
different
(6.2%),
nucleoid
thymolshapes
(4.6%),
categories
and
1,8-cineole
were (3.5%).
measured.
L. origanoidesextract protected bacterial cells against UVR-induced genotoxicity at
doses between 0.016 and 1 mg/mL; this effect was related to its major constituent pinocembrin.
Results and Discussion:
Fluorescence
microscopyThe
analysis
principal
showed
constituents
that pinocembrin
of the plant
interfered
extract with
wereE.pinocembrin
coli cell division,
(54.9%),
whattrans-?provide
caryophyllene
first
evidence on
(10.9%),
bioantimutagenic
carvacrol (6.3%),
potential
p-cimene
of the pinocembrin.The
(6.2%), thymol (4.6%),
results were
and 1,8-cineole
discussed in
(3.5%).
relation
L. to the
origanoidesextract
protected
bacterial
cells against
UVR-induced
at doses between 0.016
DNA
protective potential
of the
L. origanoides
extract
and its majorgenotoxicity
constituents.
and 1 mg/mL; this effect was related to its major constituent pinocembrin.Fluorescence microscopy
analysis showed that pinocembrin interfered with E. coli cell division, what provide first evidence on
Key words: antigenotoxicity, sos chromotest, ultraviolet radiation, lippiaoriganoides, supercritical fluid
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-052 Chemical composition of essential oil and extract obtained by supercritical fluid
extraction from Turnera diffusa and its antigenotoxicity against UVRinduced DNA
damage
Fuentes Lorenzo, Jorge Luis; Stashenko, Elena E.; Cely Coronado, Lady Johanna; Cristancho Gómez,
Rowens Andreick; Quintero Ruiz, Nathalia
Colombia
[email protected]; [email protected]
Introduction: Turnera
Turnera diffusa,
diffusa, commonly
commonlyknown
knownasasdamiana,
damiana,
is ais shrublargely
a shrublargely
used
used
as aphrodisiacs
as aphrodisiacs
in in
American traditional medicine,
medicine, but
but also
alsofor
for treatments
treatmentsofofskin,
skin,respiratory
respiratoryand
andgastrointestinal
gastrointestinaldiseases.
diseases.The
The present
present
workwork
evaluated
evaluated
the chemical
the chemical
composition
composition
of T.of diffusa
T. diffusa
essential
essential
oil (EO)
oil (EO)
andand
extract
extract
obtained by
CO2
supercritical
fluid extraction
as, itswell
DNA
effect against
radiation
obtained
by CO2 supercritical
fluid(SFE),as
extractionwell
(SFE),as
as,protective
its DNA protective
effectultraviolet
against ultraviolet
radiation (UVR)-induced
(UVR)-induced
genotoxicity.
genotoxicity.
Material and Methods: T. diffusa extracts compositions were determined by
gas chromatography-mass spectrometry (GC-MS). The antigenotoxic effect of T. diffusa EO and extract
Material
were
assayed
and Methods:
in co- incubation
T. diffusa extracts
procedures
compositions
using thewere
SOSdetermined
chromotestbyingas
Escherichia
chromatography-mass
coli PQ37 cells.
Results
and Discussion:The
major constituents
diffusa
drima-7,9(11)-diene
(28%) and
spectrometry
(GC-MS). The antigenotoxic
effect ofinT.T.diffusa
EOEO
andwere
extract
were assayed in codihydro-karanone
(25%);
in the
obtained by
mostly coli
3-deoxy-isopetasol
(64%) and 4,5-di-epiincubation procedures
using
the extract
SOS chromotest
in SFE
Escherichia
PQ37 cells.
aristolochene (22%), were detected. Both T. diffusa EO and extract obtained by SFE protected bacterial
cells
against
UVR-induced genotoxicity
in a dose-dependent
These results were
discussed
in
Results
and Discussion:The
major constituents
in T. diffusa EO manner.
were drima-7,9(11)-diene
(28%)
and
relation
to the DNA(25%);
protective
of the T.by
diffusa
EO and3-deoxy-isopetasol
extract obtained by
SFEand
and4,5-di-epitheir major
dihydro-karanone
in thepotential
extract obtained
SFE mostly
(64%)
aristolochene
constituents. (22%), were detected. Both T. diffusa EO and extract obtained by SFE protected bacterial
cells against UVR-induced genotoxicity in a dose-dependent manner. These results were discussed in
relation to the DNA protective potential of the T. diffusa EO and extract obtained by SFE and their major
Key words: turneradiffusa, essential oil, supercritical fluid extraction, antigenotoxicity, sos chromotest,
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-053 The seed of oleaginous extract of Carapa guianensis aublet a potent antioxidant
does not damage the DNA in experila models
Arencibia Arrebola, Daniel Francisco; Narciandi, Jessica; Rosario, Luis Alfredo; Delgado, Livan; Vidal
Novoa, Alexis
Instituto Finlay, Cuba
[email protected]
The majority presence of
of polyunsaturated
polyunsaturated fatty
fatty acids
acids and
andphenolic
phenolic compounds
compounds ininseed
seedoiloilextract
extractofofCarapa
Carapa guianensis
guianensis
Aublet has
Aublet
great
has
potential
great potential
as antioxidant.
as antioxidant.
But the extract
But theeffect
extract
oneffect
DNA on
it isDNA
ignored.The
it is
aim of
ignored.The
this
work wasaim
evaluated
of this work
the mutagenic
was evaluated
and genotoxic
the mutagenic
potential
and genotoxic
of the extract.The
potentialstudies
of the extract.The
were performed
in
vitro (Ames
test) with and
without
addition
theand
microsomal
hepaticoffraction,
in vivo assays
thatfraction,
included
studies
were performed
in vitro
(Ames
test) of
with
without addition
the microsomal
hepatic
on
peripheral
leukocytes
of Sprague-Dawley
rats using
the comet assay,rats
the using
micronucleus
assays
in vivo
assaysblood
that included
on peripheral
blood leukocytes
of Sprague-Dawley
the comet
and
chromosomal
aberrations
in BALB/c
mice of both
sexes administered
14both
dayssexes
orally in repeated
assay,
the micronucleus
assays
and chromosomal
aberrations
in BALB/cduring
mice of
administered
doses
(400, 1000
duringand
14 days
2000 orally
mg/kg)
in repeated
and testing
doses
of the
(400,
sperm
1000head
and 2000
morphology
mg/kg) and
of male
testing
BALB/c
of the mice
sperm head morphology
administered
during 35 days
of male
withBALB/c
doses mice
of 400,
administered
1000 and during
2000 mg/kg.
35 daysInwith
bacteria,
doses the
of 400,
extract
1000did
and
not
2000 mg/kg.
cytotoxic
and In
mutagenic.
bacteria, In
thethe
extract
in vivodidassays,
not cytotoxic
there was
and not
mutagenic.
increaseInthe
theformation
in vivo assays,
of alkalithere
labilewas
sites
notin
increase
DNA
of the
SD formation
rats leukocytes
of alkaliand
labilethere
sites was
in DNA
not ofincrease
SD rats leukocytes
the frequency
and there
of bone
was marrow
not increase
cells the
with
frequency of bone
micronucleus
and cells
marrow
withcells
chromosomal
with micronucleus
aberrations
and(structural
cells with and
chromosomal
numerical).
aberrations
aberrations
It did not increase
(structural
(structural
in and
the
and
numerical). It did
spontaneous
frequency
not increase
of anomalies
in the
the spontaneous
spontaneous
in the spermfrequency
frequency
head morphology;
of anomalies
anomalies
however,
thesperm
the
sperm
results
headof
morphology
the sperm ;
not
in
of
ininthe
head
morphology;
concentration
in the of
treated
groupconcentration
with 2000 mg/kg
the extract
were2000
different
with
the extract
negative
and
however, the results
the sperm
in the of
treated
group with
mg/kg
of the
were
positive control groups. The results indicate that this extract is not mutagenic or genotoxic according to the
performed assays.
Key words: carapa guianensis, genotoxicity assay, antioxidant
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-054 Assessment of antioxidant effect of seed oleaginous extract Carapa guioanensis
aublet using in vivo models
Curveco, Dayisell Lázara; Arencibia Arrebola, Daniel Francisco; Rosario, Luis Alfredo; Alonso, Adriana;
Delgado, Livan; Vidal Novoa, Alexis
Instituto Finlay, Cuba
[email protected]
The seed oil
oil extract
extractofofCarapa
Carapaguianensis
guianensisAublet
Aublet
is is
a natural
a natural
product
product
constituted
constituted
by abycomplex
a complex
mixture
mixture
among whose components were the poli unsaturated
unsaturated fatty acids and
and terpenoids.
terpenoids. The
The aim
aim of
of this
this work
work was
was
evaluated the potential
potential of
ofthe
theextract
extractlike
likeantioxidant
antioxidantnutritional
nutritionalsupplement
supplementin in
Sprague-Dawley
Sprague-Dawleyratsrats
withwith
future potential as antimutagenic supplements useful in support of antineoplastic therapy. Different dose of
the extract
extract 100,
100, 200
200and
and400
400mg/kg
mg/kgwere
wereadministered
administered
in co-treatment
in co-treatment
withwith
Cyclophosphamide
Cyclophosphamide
(CP)(CP)
a a
potent inductor of
of oxidative
oxidative stress
stressand
andititwas
wasalso
alsoadministered
administeredthe
themaximum
maximumdose
doseofof
the
the
product
product
400
400
mg/kg.
mg/kg.
In
all the
In cases
all the were
casesdetermined
were determined
the influence
the influence
of these
of treatments
these treatments
in the in
water
the water
and foods
and foods
intake,intake,
corporal
corporal
weight, haematology
and biochemistry
sanguine
the plasmatic
antioxidant
The
weight, haematology
and biochemistry
sanguine
test andtest
theand
plasmatic
antioxidant
state.state.
The obtained
obtaineddemonstrated
results
results demonstrated
that the consumption
that the consumption
of the extract
of the
in co-treatment
extract in co-treatment
with the CP
with
didthe
notCP
affect
did the
not water
affectfoods
and
the water
intake
andneither
foods intake
the increase
neither the
the increase
corporal the
weight
corporal
when
weight
200 when
mg/kg
200
doses
mg/kgwere
doses
used.
were The
used. The haematological
haematological
and biochemical
and biochemical
parametersparameters
analyzed were
analyzed
not affected
were notfor
affected
none of
forthe
none
evaluated
of the doses,
evaluated
being
abledoses,
to correct
being
theable
hepatotoxicity
to correct the
caused
hepatotoxicity
by the drug
caused
and the
by the
disorders
drug and
in the
the lipid
disorders
metabolism.
in the lipid
The
metabolism.
seed
oleaginous
The seed
extract
oleaginous
of Carapaextract
guianensis
of Carapa
Aublet
guianensis
was a good
Aublet
antioxidant
was a good
that antioxidant
which demonstrated
that whichin
demonstrated
the
reported values
in theofreported
enzymatic
values
activity,
of enzymatic
of the main
activity,
biomarker
of theofmain
oxidative
biomarker
stressofused.
oxidative
With stress
these results,
used.
we
thewebeginning
of the
studies
toxicology
studies)
and thestudies)
preclinical
Withrecommend
these results,
recommend
the security
beginning
of the (preclinical
security studies
(preclinical
toxicology
antimutagenic studies of the extract useful during the antineoplastic therapy.
Key words: carapa guianensis aublet, antioxidant, in vivo, antimutagenesis, cyclophosphamide
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-055 Pharmacognostical study of Schinus terenbinthifolius Raddi (false copal). Tkalenko
CA.
Águila Tkalenko, Clara; Álvares Hernández, Yarelys; Mena Álvarez, Odaimi; Milanés Fernández,
Marlen
Empresa de Productos Inyectables LABIOFAM, Cuba
[email protected]
The extracts
extracts hydro-alcoholic
hydro-alcoholic obtained
obtainedstarting
startingfrom
fromthethe
leaves
leaves
of Schinus
of Schinus
terebinthifolius
terebinthifolius
Raddi
Raddi
(false(false
copal), are
are broadly
broadly used
usedbybytheir
their
anti-inflammatory
anti-inflammatory
andand
antimicrobial
antimicrobial
properties.
properties.
Our Our
objective
objective
was was
to to
develop a farmacognóstico study
study of
of Schinus
Schinus terebinthifolius
terebinthifolius Raddi
Raddi(false
(falsecopal).
copal).Taxonomicthe
Taxonomicthebotanical
botanical
species was
was classified
classifiedand
andit it
evaluatedthethe
morphology
of leaves
the leaves
visually.
The drying
study was
evaluated
morphology
of the
visually.
The drying
study was
developed under different conditions being
being measured
measured humidity,
humidity, drying
drying temperature
temperature and
and loss
lossof
ofweight.
weight.The
vegetable
dry material
was was
stored
in flask
of glass
amber,
bags bags
of paper
kraft kraft
and polyethylene
bags bags
being
The vegetable
dry material
stored
in flask
of glass
amber,
of paper
and polyethylene
being evaluated
evaluated
humidity,
humidity,
characteristic
characteristic
organoleptic
organoleptic
and microbial
and microbial
load. Starting
load. Starting
from anfrom
extract
an extract
hydro- hydroalcoholic
alcoholic
to
80% obtained
to 80% by
obtained
maceration
by maceration
secondarysecondary
metabolites
metabolites
were identified
were identified
using the using
technique
the technique
of Rondinaofand
Rondina and
Coussio
(1969)
Coussio
modified
(1969)
by modified
Durand (1986).
by Durand
To (1986).
this extract
To this
they
extract
werethey
determined
were determined
physical-physicalchemical
chemical parameters:
parameters:
characteristic
characteristic
organoleptic,
organoleptic,
pH, relative
pH, density,
relative density,
and refraction
and refraction
index, alcoholic
index, alcoholic
content and
content
total
solids.
and Metabolites
total solids. Metabolites
of interest were
of interest
identified
wereby
identified
chromatographic
by chromatographic
in thin layer.
in thin
Total
layer.
phenols
Total were
phenols were
quantified
by visible
quantified
UV to
by765
visible
nm.UV
Thetoresults
765 nm.
threw
The that
results
it was
threw
about
thatthe
it was
species
about
Schinus
the species
terebinthifolius
Schinus
terebinthifolius
Raddi
in correspondence
Raddi in correspondence
with the consulted
with the
bibliography.
consulted The
bibliography.
solar dryer
Theand
solar
thedryer
nylonand
bags
thewere
nylonthe
bags were
most
efficient
the methods
most efficient
for the
methods
obtaining
for the
andobtaining
storage and
of the
storage
vegetable
of thedry
vegetable
material.
drySecondary
material. several
Secondary several
metabolites
was identified
metabolites
in the
wasextract
identified
hydro-alcoholic
in the extract(pH:
hydro-alcoholic
5.30, density:
(pH:0.8902,
5.30, density:
total solids:
0.8902,
8.4%,
refraction
index:
alcoholic
content:alcoholic
72%), steroids,
alkaloids,resins,
phenols
and tannins
being
total solids:
8.4%,1.3786,
refraction
index: 1.3786,
content:resins,
72%), steroids,
alkaloids,
phenols
obtained, a 7.88% of total phenols agreeing with that obtained by other authors.
Key words: schinus terebinthifolius raddi, farmacognóstico, physical-chemical parameters
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-056
Extraction of flavonoids from leaves of Annona muricata L.
Henry García, Yaima; Salomón Izquierdo, Suslebys; Acosta Esquijarrosa, Jhoanny; Romero Díaz,
Aylema; López de Armas, Marilyn; Mercado Vidal, Josué
EPVVB, Grupo Empresarial LABIOFAM, Cuba
[email protected]
The soursop (Annona muricata L)
L) is used
used in
in the
the ethno-medicine
ethno-medicine for
for the
thetreatment
treatmentof
ofdifferent
different diseases.
diseases.The
The presence
presence
of flavonoids
of flavonoids
in the
in leaves
the leaves
havehave
beenbeen
determined
determined
which
which
havehave
antidiabetic,
antidiabetic,
antioxidant
antioxidant
andand
antianti-inflammatory
properties.
study
to evaluate
influence
of operational
parameters
inflammatory
properties.
The The
study
aimsaims
to evaluate
the the
influence
of operational
parameters
in in
obtaining
obtaining extraction
extraction
of flavonoids
of flavonoids
from the leaves
from the
of leaves
Annonaofmuricata
Annona muricata
L . Materials
L . Materials
and Methods:
and Methods:
The evaluation
The of
evaluation
the
operating
of the
variables
operating
was
variables
performed
was using
performed
an experimental
using an experimental
design ofdesign
response
of response
surface surface
rotational
rotational comprised
comprised
central 2 central
^ 3 with2 star
^ 3 with
points
star, points
being selected
, being selected
the intervals
the intervals
between
between
50 and5070mL
and 70mL
/g for /g
the
for the relation
relation
vegetal-volume
vegetal-volume
materialmaterial
of solvent
of solvent
, between
, between
40 and40
80%
andthe
80%
ethanol
the ethanol
concentration
concentration
and between
and
between
2
and 3 h2the
andextraction
3 h the extraction
time. The time.
quantification
The quantification
of total flavonoids
of total flavonoids
was performed
was performed
by a colorimetric
by a
method
colorimetric
at
430 nm, expressed
method at 430
as quercetin.
nm, expressed
Results
as and
quercetin.
Discussion:
Results
theand
presence
Discussion:
of flavonoids
the presence
and quercetin
of
in
flavonoids
the
extracts
andwas
quercetin
determined.
in the extracts
The significant
was determined.
variables The
resulted
significant
the quadratic
variables effects
resultedofthe
plant-material
quadratic
effects of plant-material
relationship
solvent volume
relationship
and thesolvent
concentration
volume and
of ethanol.
the concentration
The optimum
of ethanol.
extraction
The condition
optimum in total
extraction condition
flavonoids
was obtained
in total
at a
flavonoids
concentration
was obtained
of ethanolatofa 96%,
concentration
a ratio of70
of ethanol
mL/ g and
of 96%,
a timea of
ratio
1.6h,
of70
to mL/
yield
g andprocess.
87%
a time ofTo1.6h,
the best
to yield
experimental
87% process.
condition
To thetriplicate
best experimental
experimentscondition
were performed
triplicate and
experiments
comparedwere
with
performed
the
value predicted
and compared
by thewith
design,
the value
a good
predicted
correlation
by thewas
design,
founda good
between
correlation
them. Conclusions:
was found between
The best
extraction
conditionsThe
were
selected
in obtaining
extract
from the
leaves of
with the
presence
them. Conclusions:
best
extraction
conditionsanwere
selected
in obtaining
ansoursop
extract from
leaves of
flavonoids.
Key words: annona muricata l, flavonoids, solid-liquid extraction
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-057 Study in silico of the DNA protective effects of the 2,6 –disecbutilphenol extracted
from the plant Phyllanthus orbicularis K
Castanedo Monteserín, Lázaro Andrés ; Marrero Gutiérrez , J.; Lamar Sánchez, A.
Universidad de La Habana (UH), Cuba
[email protected]
The appearance of chronical degenerative deseases has a close relationship with the DNA damage in
somatic cells. The damage can be originated by different genotoxic agents, as for example the aromatic
Introduction:
amines.
At the present
it’s considered
as deseases
possible way
prevent
and treatwith
these
the use
The
appearance
of chronical
degenerative
has to
a close
relationship
theaffections
DNA damage
in of
chemoprotective
fitocompounds. It has been studied that the total aqueous extract of the cuban specie
somatic
cells.
Phyllanthus
K reduce
significantly
the damage
by the aromatic
amines:
m-PDA), 4The
damage Orbicularis
can be originated
by different
genotoxic
agents,induced
as for example
the aromatic
amines.
aminobiphenyl
(4-ABP)
y 2-amino1-methyl-6-phenylimidazo[4,5-b]py
(PhIP)
and offrom the total
At
the present it’s
considered
as possible
way to prevent and treat theseridine
affections
the use
aqueous fractioned
extract was isolated and identified the 2,6 – disecbutilphenol (DSBP). However, it is not
chemoprotective
fitocompounds.
how studied
the DSBP
at aqueous
a molecular
level.ofThank’s
to the
the advances
in Orbicularis
computational
chemistry
Itknown
has been
thatprotects
the total
extract
the cuban
specie
Phyllanthus
K reduce
and modelations
tools is posible
the directamines:
chemicalm-PDA),
interaction
between the DSBP
andy the
aromatic
significantly
the damage
inducedtobystudy
the aromatic
4-aminobiphenyl
(4-ABP)
2-aminoamines as possible genoprotective mechanism.
The from
differents
association
between
the phenol
1-methyl-6-phenylimidazo[4,5-b]pyridine
(PhIP) and
the total
aqueous complexes
fractioned extract
was
and the and
aromatic
amines
were– disecbutilphenol
generated by Multiple
Minimum Hypersurface (MMH) and optimized with
isolated
identified
the 2,6
(DSBP).
the semiempirical
methods
AM1
PM6
– d and
The association
energies
complex
However,
it is not known
how
the and
DSBP
protects
at PM7.
a molecular
level. Thank’s
to thefor
theeach
advances
in were
obtaining with the
program
Q3.
The resultstools
allowed
demonstrated
thatdirect
the association
between the
phenol
computational
chemistry
and
modelations
is posible
to study the
chemical interaction
between
andDSBP
the amines
was
thermodynamically
favored genoprotective
and it also can mechanism.
be predicted a genoprotective order for the
the
and the
aromatic
amines as possible
phenol against
each amine.This research work allow us to propose the chemical direct interaction between
Materials
and Methods:
the phenol
andassociation
the aminescomplexes
as possiblebetween
molecular
DNA protection.
The
differents
themechanism
phenol and for
thethe
aromatic
amines were generated by
Multiple Minimum Hypersurface (MMH) and optimized with the semiempirical methods AM1 and PM6 – d
and PM7. The association energies for each complex were obtaining with the program Q3.
Results:
The results allowed demonstrated that the association between the phenol and the amines was
thermodynamically
and
it also canorbicularis
be predicted
a genoprotective
orderphip
for the phenol against
Key words: dna,favored
in sílico,
phyllanthus
k, dsf,
m – pda, 4 - abp,
each amine
amine.
Conclusions
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-058
Malpighia glaba L. (Acerola): analytical studies
García Peña, Caridad Margarita; García Borges, Lisandra; Martínez Espinosa, Vivian; Nogueira,
Antonio; González, Maria Lidia
Centro de Investigación y Desarrollo de Medicamentos (CIDEM), Cuba
[email protected]
Introduction: Malpighia glaba L. (Acerola, Miguel variety) has beneficial health virtues, is used in traditional
medicine
as an
immunostimulant,
detoxifying,
remineralizing,
among others.
studies
Introduction:
Malpighia
glaba L. (Acerola,
Miguel
variety) has beneficial
healthMethods:
virtues, isagricultural
used in traditional
were
conducted
at the Experimental
Station remineralizing,
of Medicinal Plants
JuanMethods:
Tomas Roig
of thestudies
Drugs
medicine
as an immunostimulant,
detoxifying,
amongDr.
others.
agricultural
Research
and Development
Center Station
(CIDEM),
determining
the Dr.
optimal
time. of
While technological
were conducted
at the Experimental
of Medicinal
Plants
Juan harvest
Tomas Roig
the Drugs
and
analytical
studies wereCenter
conducted
in the
researchthe
department
belonging
to thetechnological
same center,
Research
and Development
(CIDEM),
determining
optimal harvest
time. While
demonstrating
throughwere
a chromatographic
method
by High
Performance
Liquid
Chromatography
and analytical studies
conducted in the
research
department
belonging
to the
same center, (HPLC),
applicable
to the
qualityacontrol
and stability
study the
high content
of Vitamin
which is attributed
to their
demonstrating
through
chromatographic
method
by High
Performance
LiquidC,Chromatography
(HPLC),
therapeutic
Simultaneously
studies
identification
by Thin
Layer
Chromatography
applicable toeffects.
the quality
control and phytochemical
stability study the
high for
content
of Vitamin
C, which
is attributed
to their
(CCD)
of different
in the
plant material
is performed
in addition
specifications
established
therapeutic
effects. components
Simultaneously
phytochemical
studies
for identification
by Thin
Layer
quality
finished product
the method
of K tabulated.
Results:
agricultural
studiesinidentified
Chromatography
(CCD)using
of different
components
in the plant
material
is performed
addition the best time
of
year to harvest
the plant
material.
Theproduct
methodusing
for controlling
and stability
studyagricultural
was specific,
specifications
established
quality
finished
the methodquality
of K tabulated.
Results:
accurate,
linear, accurate
in the
concentrations
studies quality
provedand
the
studies identified
the best time
of range
year toofharvest
the plant studied.
material. The
The phytochemical
method for controlling
presence
of saponins
and flavonoids
in plant
material.
Conclusions:
a raw materialstudied.
of natural
stability study
was specific,
accurate, linear,
accurate
in the
range of concentrations
Theorigin that
meets
quality specifications
required
for the development
of pharmaceutical
products
was Conclusions:
obtained.
phytochemical
studies proved
the presence
of saponins and
flavonoids in plant
material.
a
Key words: Malpighia glaba L., Acerola, analytical study
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-059 Effect of dietary supplementation with sanguinarine on biological indicators in
growing
Martínez Aguilar, Yordan; Liu, G.; Yin, Y. L.; Rodríguez, R.; Valdivié, M.; Más, D.
Universidad de Granma, Cuba
[email protected]
This study was conducted to determine the effect
effect of
of dietary
dietary supplementation
supplementation with
with sanguinarine
sanguinarine on
on growth
growt h
performance, serum
serummetabolites
metabolites
and and
antibodies
antibodies
in growing
in growing
pigs. A total
pigs.
of 120
A total
[Yorkshire×
of 120 [Yorkshire×
(Duroc×Landrace)] pigs,
pigs, at
at 66weeks
weeksofofage
age(3(3
weeks
weeks
postweaning)
postweaning)
were
were
randomly
randomly
allotted
allotted
to 3to
dietary
3 dietary
treatments. The dietary treatments were: the basal
basal diet
diet (BD)-fed
(BD)-fed group,
group, the
the sanguinarine
sanguinarine (sangrovit®)-fed
(sangrovit®)-f ed
group (BD+0.04 g/kg), and the antibiotic-fed group
group (BD+0.2
(BD+0.2 g/kg
g/kg colistin)
colistin) (n=40
(n=40 per
per group).On
group).On days
days 7,
7,14
14
and 28 after
after initiation
initiationofoftreatment,
treatment,serum
serumlevels
levelsofofamino
amino
acids
acids
(AA),
(AA),
antibodies
antibodies
andand
some
some
biochemical
biochemical
parameters were
were measured.
measured. Sanguinarine
Sanguinarine used
usedasassupplemental
supplementalincreased
increased(P<0.05)
(P<0.05)
thethe
average
average
daily
daily
feed intake (ADFI),
(ADFI), average
average daily
daily gain
gain(ADG),
(ADG),nutrient
nutrientintakes
intakesand
andfinal
finalbody
bodyweight
weight
(BW),
(BW),
improved
improved
thethe
feed/gain ratio (F/G) (P<0.05), and
and reduced
reduced (P<0.05)
(P<0.05) the
the incidence
incidence of
ofdiarrhea
diarrhea iningrowing
growing pigs
pigscompared
compared
with the non-additive and antibiotic groups. Results indicated that the sanguinarine increased (P<0.05) the
serum
contents
of AA,
such
asasGly,
Ala and
and Thr
Thrinincomparison
comparisonwith
with
antibioticthe serum
contents
of AA,
such
Gly,Ile,
Ile,Lys,
Lys,Met,
Met, Arg, Ala
thethe
antibioticsupplemented group and/or BD
BD on
on days
days of
of trial.
trial. InIncontrast
contrasttotocolistin,
colistin,sanguinarine-fed
sanguinarine-fed group
groupdecreased
decreased
(P<0.05) serum
serum level
level ofoftriglycerides
triglyceridesand
andincreased
increased
(P<0.05)
concentration
of glucose,
as well
as this
(P<0.05)
concentration
of glucose,
as well
as this
medicinal extract enhanced (P<0.05) serum concentration of IgG, but only on day 7 in relation to the other
2 groups. These
These findings
findings indicate
indicateaabeneficial
beneficialeffect
effectofofthe
the
sanguinarineonon
growth
performance,
serum
sanguinarine
growth
performance,
serum
concentration of some essential and non-essential AA, humoral immune response and diarrhea
incidence, which may offer an effective alternative to antibiotics for growing pigs.
Key words: sanguinarine, pig, amino acid, serum antibody, serum metabolite, growth performance
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-060 Development and validation of a high performance liquid chromatographic method
to the quantitation of phytosterols in Portulaca oleracea L
Alba de Armas, Miguel Ángel; Bravo Sánchez, Luis Ramón; Dumenigo Peña, Lismeivy; Gómez Reyes,
Imilsys
Universidad Central "Marta Abreu" de Las Villas (UCLV), Cuba
[email protected]
Purslane (Portulaca oleracea L.) is
is aa widely
widely extended
extended plant
plantininCuban
Cuban fields
fields and
anditsitsuse
useforforalimentary
alimentaryand
and
medicinal purposes
purposes isiswell
wellknown.
known.Traditionally
Traditionally
it has
employed
as diuretic,
anti-helmintic,
it has
beenbeen
employed
as diuretic,
anti-helmintic,
anti- antiinflammatory, etc.
etc. ItIt isisreported
reportedthat
that
Portulaca
oleracea
L. can
reduce
atherosclerosis
prolonged
Portulaca
oleracea
L. can
reduce
atherosclerosis
after after
prolonged
hypercholesterolemia due to the presence of phytosterols which are able to inhibit the intestinal absorption
of
cholesterol.
In this work,
method
High Performance
Liquid Chromatography
(HPLC) for(HPLC)
qualitative
absorption
of cholesterol.
In athis
work, by
a method
by High Performance
Liquid Chromatography
for
and
quantitative
analysis ofanalysis
stigmasterol
and ß-sitosterol
in Portulaca
oleracea L.
powder L.
was
developed.
qualitative
and quantitative
of stigmasterol
and ß-sitosterol
in Portulaca
oleracea
powder
was
A
Lichrospher
RP-18 (5 µm)
125 (5X µm)
4 mm
column (reversed
phase); isocratic
developed.
A Lichrospher
RP-18
125chromatographic
X 4 mm chromatographic
column (reversed
phase); operation
isocratic
mode
and mode
UV detection
a wave at
length
of 208
nmof
were
method was
validated
operation
and UV at
detection
a wave
length
208 set.
nm The
weredeveloped
set. The developed
method
was for the
quality
control
ofquality
the mentioned
powders by
means
of thebyfollowing
parameters:
linearity,
precision,
validated
for the
control ofplant
the mentioned
plant
powders
means of
the following
parameters:
accuracy,
specificity
and detection
/ quantification
as described
inasinternational
The
linearity, precision,
accuracy,
specificity
and detectionlimits,
/ quantification
limits,
described instandards.
international
developed
allowed
to simultaneously
determining stigmasterol
andstigmasterol
ß -sitosterol in
standards. and
The validated
developedtechnique
and validated
technique
allowed to simultaneously
determining
Portulaca
oleracea
L. powders.
A 0,243L.%
(dry base)
of stigmasterol
and of
0,815
% (dry base)
of ß-sitosterol
and ß-sitosterol
in Portulaca
oleracea
powders.
A 0,243
% (dry base)
stigmasterol
and 0,815
% (dry
were
obtained
from
quantitative
analysis.
The
HPLC
method
was
reliable
and
could
be
employed
as a
base) of ß-sitosterol were obtained from quantitative analysis. The HPLC method was reliable and could
quality control method.
Key words: phytosterols, hypercholesterolemia, hplc, validation, portulaca oleracea l
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-061
Toxicity studies of Curcuma longa extracts
Quintana Dovales, Neisa ;García Martín, Juan de Jesús
Laboratorio de Fito y Apifármacos (Pinar del Río), Cuba
[email protected]
The CUMIN EXTRACT is a pharmaceutical form prepared from the Curcuma longa L.. The Curcuma longa
L.
is harvested
from the north-east
of Pinar delform
Rio prepared
province, from
Cuba.the
From
1995 was
isolated
antirretroviral
The
CUMIN EXTRACT
is a pharmaceutical
Curcuma
longa
L.. TheanCurcuma
protein
of 18-19D
molecular
weightdel(Garcia
Martin J.
et al From
2003.NPU).Toxicity
in Curcuma
longa L.(Tyrphostines)
is harvested from
the north-east
of Pinar
Rio province,
Cuba.
1995 was isolated
an
longa
extractsprotein
was our
principal objective
in this
case. In weight
first place
biological
antirretroviral
(Tyrphostines)
of 18-19D
molecular
(Garcia
Martinassays
J. et al were realized using
40
female mice frominSwitzerland
Stockextracts
of-1 (Barcelona),
with maintenance
Extract of
2003.NPU).Toxicity
Curcuma longa
was our principal
objective in diet
this using
case. Aqueous
In first place
Curcuma
Twenty
mice using
received
extract mice
and twenty
were the control
This group with
received a
biological longa
assaysL.were
realized
40 female
from Switzerland
Stock group.
of-1 (Barcelona),
standard
diet.diet
Experimental
studies
with 4mg/kg
mouse
per L.
day=
0.4 mg/Kg
mouse per
day and
withtwenty
curcumin
maintenance
using Aqueous
Extract
of Curcuma
longa
Twenty
mice received
extract
in
human
doses.group.
The evaluation
to the
mice was
in the Thomas
measure,
weight
were
the control
This group made
received
a standard
diet.about
Experimental
studies media
with 4mg/kg
mouse
per
sequence,
muscular
coordination
method,
String-Test
Miguel The
Blasco
( Don't fall
in atorow),
Spontaneous
day= 0.4 mg/Kg
mouse
per day with
curcumin
in humanof doses.
evaluation
made
the mice
was
explorer
(Labyrinth
in T:measure,
explore the
first sequence,
arm of the muscular
labyrinth in
60 seconds).
Passing
four weeks
about in the
Thomastest
media
weight
coordination
method,
String-Test
of the
mice
sacrificed
andinwe
take Spontaneous
blood from the
neck, (Labyrinth
a piece oftest
liverin (Lipid
peroxidation)
looking
Miguelwere
Blasco
( Don't fall
a row),
explorer
T: explore
the first arm
of thefor
Malonialdehyde
concentration
by thiobarbituryc
and sacrificed
proteins concentrated
Lowry
As aa
labyrinth in 60 seconds).
Passing
four weeks themethod
mice were
and we take by
blood
frommethod.
the neck,
result
of liver
this research
we obtained
that Curcuma
longa extracts
didn't cause
toxicity in method
mice, besides
piece of
(Lipid peroxidation)
looking
for Malonialdehyde
concentration
by any
thiobarbituryc
and
decreased
lipids peroxides
in blood
and finally
the 25ofhigher
doses ofweCurcuma
not
toxics. longa
proteins concentrated
by Lowry
method.
As a result
this research
obtainedare
that
Curcuma
Key words: toxicity studies, Curcuma longa, cumin
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-062 Functional Characterization of an antifungal peptide from marine invertebrate
Cenchritis muricatus (Gastropoda: Littorinidae)
Lugo Álvarez, María Bárbara; Alba Menéndez, Annia; Garay, Hilda E.; Campos Dias, Simoni; Luiz
Franco, Octavio; López Abarrategui, Carlos; Otero González, Anselmo J.
Institute of Tropical Fruit Growing, Cuba
[email protected]
The search of new
new molecules
molecules from
from natural
natural sources
sources to
totreat
treat infectious
infectious and
andparasitic
parasiticdiseases
diseaseshas
haspointed
pointed
antimicrobial peptides as a promissory alternative.
alternative. Besides,
Besides, the
the important
important role
role of
ofthese
thesepeptides
peptides inininnate
innate
immunity, especially
especiallyinininvertebrates,
invertebrates,makes
makes
this this
groupgroup
of animals
of animals
a secure
a source
secure for
source
obtaining
for obtaining
antimicrobial peptides. Due
Due to
to the
thedifferent
different characteristics
characteristicsand
andversatility
versatilityininfunctions
functionsof of
these
these
molecules
molecules
and the continuous development
development of
of resistance
resistance to
toconventional
conventional antibiotics
antibioticsininmany
manymicrobial
microbialstrains;
strains;the
theaim
of
to improve
the antimicrobial
activity
of Cm-p1,
a peptide
isolated
of the
mollusk
Cenchritis
aimthis
of work
this work
to improve
the antimicrobial
activity
of Cm-p1,
a peptide
isolated
of sea
the sea
mollusk
muricatus.
It was performed
from synthetic
peptides from
Cm-p1taking
into account
Cenchritis muricatus.
It was performed
from derivative
synthetic derivative
peptides
from Cm-p1taking
intoproperties
account
properties
such
as Boman
such as
index,
Boman
cationicity
index, and
cationicity
mass weight
and mass
of the
weight
original
of the
fragment
originaloffragment
Cm-p1, parameters
of Cm-p1, currently
parameters
related
to antimicrobial
currently related
activity.
to In
antimicrobial
order to accomplish
activity. Inour
order
objective,
to accomplish
the antibacterial
our objective,
and antifungal
the
of the
antibacterial
three
synthetic
andpeptides
antifungal
Cm-p3,
of theCm-p4,
three synthetic
Cm-p5 were
peptides
evaluated
Cm-p3,
and
Cm-p4,
compared
Cm-p5
with
were
Cm-p1.
evaluated
They showed
and
compared
a
little and with
null Cm-p1.
antibacterial
They activity
showedinacontrast
little andtonull
antifungal
antibacterial
activity
activity
which
in was
contrast
increased
to antifungal
mostlyactivity
Cm-p5.
whichpeptide
This
was increased
resulted, mostly
conveniently,
Cm-p5. non-toxic
This peptide
for animal
resulted,
cells
conveniently,
in vitro, at relatively
non-toxichigh
for animal
concentrations.
cells in vitro,
Key words: antibacterial activity, antifungal activity, antifungals, minimum inhibitory concentration
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-063 Applications of essential oil clove as fungicide on environments of archives and
libraries
Valdés Pérez, Oderlaise; Borrego Alonso, Sofía Flavia; Anaya Villalpanda, Matilde; Vivar González,
Isbel; Molina Veloso, Alian; Rodrìguez, Julio C.
Archivo Nacional de la República de Cuba, Cuba
[email protected]
The preservation of
of historical
historical memory
memory has
hasbeen
beena apriority
priorityof of
allall
civilization.
civilization.
Archives
Archives
andand
libraries
libraries
hoard
hoard
much of the
the documentary
documentary heritage
heritage on
onpaper.
paper.Though,
Though,sometimes
sometimesthere
thereareare
problems
problems
of biodeterioration
of biodeterioration
caused by
by fungi,
fungi,conditioned
conditionedmainly
mainlyby by
fluctuations
fluctuations
of environmental
of environmental
parameters
parameters
whichwhich
promoted
promoted
the the
development thereof. For this in the
the biodeterioration
biodeterioration control,
control, chemicals
chemicals have
have been
been used,
used,although
althoughitithas
hasnot
been
a definitive
solution,
as as
these
staff, the
thedocument
documentand
andthe
the
environment ,
not been
a definitive
solution,
thesemay
mayaffect
affect the
the health
health of staff,
environment,
therefore is continued in the search for safer products.
products. At present,
present, scientific
scientific studies
studies are
are conducted
conducted in
in order
to
findtoalternatives
for controlling
biodeterioration
by using
plant plant
products
as biocides.
The The
present
study
order
find alternatives
for controlling
biodeterioration
by using
products
as biocides.
present
study to
aims
aims
evaluate
to evaluate
the antifungal
the antifungal
activityactivity
of the of
essential
the essential
oil of clove
oil of clove
against
against
three three
strainsstrains
from environment
from
s
environments
of
the repositories
of theofrepositories
the NationalofArchives
the National
of the
Archives
Republic
of the
of Cuba
Republic
and its
of Cuba
effect and
on paper.
its effect
Theonfollowing
paper.
The followingwere
procedures
procedures
used: the
were
wells
used:
used:
method
the
the wells
and
wellsthe
method
method
tube and
dilution
and the
thetube
method,
tubedilution
dilution
biofilm
method,
method,
inhibition
biofilm
biofilm
test
inhibition
inhibition
and biocidal
test
test
and biocidal
activity
test on
activity
papertest
colonized
on paperbycolonized
colonized
fungi. Onby
paper
fungi.
fungi.was
On measured
paper
paper was
was the
measured
measured
copper the
the
number,
copper
copper
the
number,
number,
pH value
the
theand
pH
pH
value
the
appearance
and the appearance
of stains before
of stains
andbefore
after accelerated
and after accelerated
again. The again.
resultsThe
showed
results
biocidal
showed
activity
biocidal
to values
of
3% oftothe
oil, affectation
in the
theconidiogenesis,
formation of biofilms
was inhibited
as well
as inhibited
the fungal
activity
values
of 3% of the
oil,conidiogenesis,
affectation in the
the formation
of biofilms
was
colonization. Also, there was no significant changes in the paper pH, no structural changes in the paper, or
stains on them.
Key words: essential oils, vegetable biocide, fungicidal activity, biodeterioration, documentary heritage
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-064 Hypocholesterolemic and antiatherosclerotic effects of crude leaf extract of Moringa
oleifera in Apoe Ko Mice
Fernández, Nidia; Fuentes, D.; Díaz, B. L.; León, A.; Blanco, D.; Gutiérrez, M.; García, A.; Ronda, M.;
Prida, Y.; Torres, Y.; Cabezas, L.
Centro Nacional para la Producción de Animales de Laboratorio (CENPALAB), Cuba
[email protected]
elevated
concentration
of total
in plasma
with
Numerous population
population studies
studieshave
havelinked
linked
elevated
concentration
of cholesterol
total cholesterol
in plasma
with
oleifera Lam (Moringaceae)
(Moringaceae) are
are used
increased incidence of atherosclerotic events. The leaves of Moringa oleifera
used
in
many
in many
cultures
cultures
as as
a hypocholesterolemic
a hypocholesterolemicagent
agent inin obese patients.
patients.Taking
Takinginto
into
consideration
consideration
thatthat
B6.129P2-Apoetm1Unc/J mice
miceexhibit
exhibita marked
a marked
increase
increase
in plasma
in plasma
cholesterol
cholesterol
level and
level
develop
and develop
atherosclerotic lesions resembling those
those seen
seen in
in humans;
humans; the
the main
mainobjective
objective ofofour
ourstudy
studywas
wastotoevaluate
evaluat e
the hypocholesterolemic and anti-atherosclerotic effects of crude extract
extract of
of leaf
leaf of
of Moringa
Moringa oleifera
oleifera in
in ApoE
ApoE
KO
mice.
KO Males
mice. Males
and females
and females
B6.129P2-Apoetm1Unc/J
B6.129P2-Apoetm1Unc/J
KO with
KO4 with
- 8 weeks
4 - 8 weeks
of age of
were
agefed
were
ad fed
libitum
ad with
libitum
diet.
Theanimals
half of the
fed leaf
with extract
crude leaf
extract of
Moringa
oleifera
during
normal with
diet.normal
The half
of the
wasanimals
fed withwas
crude
of Moringa
oleifera
during
90 days
and
90 days
the other
control. C57Bl/6
miceused
wereas
used
as control.
weight,
the
otherand
served
as served
control.asC57Bl/6
mice were
control.
Body Body
weight,
foodfood
consumption,
consumption, haematological
andparameters,
biochemicalrelative
parameters,
weightand
of the
organs
and aortic lesions
haematological
and biochemical
weight relative
of the organs
aortic
atherosclerotic
atherosclerotic
It was found
that
administration
crude oleifera
leaf extract
of
were
measured.lesions
It was were
foundmeasured.
that administration
of the
crude
leaf extractofofthe
Moringa
decreased
the
Moringa oleifera
decreased
in comparison
with control
cholesterol
increases
in ApoE
theKO
cholesterol
mice serum
increases
in comparison
in ApoE KO
withmice
control
serum
group,
while no changes
were
group, inwhile
no changes
plaque
formation
in aorta
found
wild type
mice (C57Bl/6).
were found
Atherosclerotic
in wild type mice
plaque
(C57Bl/6).
formationAtherosclerotic
in aorta was also
reduced
approximately
was
alsoinreduced
35 % inIt treated
ApoE KO mice.
wasleaves
concluded
that the leaves
of have
35 %
treated approximately
ApoE KO mice.
was concluded
that Itthe
of Moringa
oleifera
Moringa oleifera have and
hypocholesterolemic
hypocholesterolemic
anti-atherosclerotic
andactivity
anti-atherosclerotic
in B6.129P2-Apoetm1Unc/J
activity in B6.129P2-Apoetm1Unc/J
mice and that there is
valid pharmacological basis for employing them for this purpose in humans.
Key words: atherosclerosis, apoe mice, hypocholesterolemic, moringa oleifera
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-065
Effect of Korean native chicken meat on pro-inflammatory cytokine gene expression
Hye Kyung, Kim; Myung Gyou, Kim; Kang Hyun, Leem
Hanseo University, Turkey
[email protected]
Introduction: Chicken essence is regarded as
as aa health
health food
food in
in Chinese
Chinese communities
communities and
and South
South East
EastAsia.
Asia.
In western countries, chicken soup
soup has
has also
also been
been traditionally
traditionally used
usedfor
forrecovery
recovery from
fromcold.
cold.Present
Presentstudy
study
investigated the anti-inflammatory effects
effects of
of four
four different
different Korean
Korean native
native chickens
chickens(YeonsanOgye,
(YeonsanOgye, Hyunin
Hyunin
Black, Hwangbong,
Hwangbong, and
andHoengseongYakdak).
HoengseongYakdak).White
White
Leghorn
Leghorn
waswas
usedused
as a as
control.
a control. Materials and
Materials and
Methods:
Breast
Methods:
meatsBreast
were meats
grounded
were and
grounded
extracted
and extracted
with 70%with
ethanol
70% ethanol
at 60 oC
at 60
foroC6 for
hours.The
6
hours.The concentrationof
concentrationof
released histamine
released was
histamine
determined
was determined
in compound
in compound
48/80-induced
48/80-induced
HMC-1 cellHMC-1
supernatant
cell s
supernatants
by
spectrofluorometric
by spectrofluorometric
procedure. Quantitative
procedure. real-time
Quantitative
polymerase
real-time chain
polymerase
reactionchain
(RT-reaction
PCR) was
(RT-used
PCR)
to
quantify
was used
the expression
to quantifyofthe
mRNAs
expression
for IL-4,
of mRNAs
IL-10, IFNfor IL-4,
, andIL-10,
TNF-?IFNin LPS-stimulated
, and TNF-? in RAW
LPS-stimulated
264.7 cells.
RAW 264.7
Results:
Histamine
cells. release was not affected by all strains of breast or leg meat extracts. However, breast
Results:
meat
extracts
Histamine
of fourrelease
strainswas
of Korean
not affected
nativebychicken
all strains
significantly
of breast or
reduced
leg meat
theextracts.
gene expressions
However, breast
of IL-4
meatIFNand
extracts
while
of White
four strains
Leghorn
of Korean
fail to affect
nativethese
chicken
parameters.
significantly
IL-10
reduced
mRNAthe
expressions
gene expressions
were decreased
of IL-4
andHwangbong
IFN- while White
Leghorn fail to affect breast
these parameters.
IL-10
mRNA
were decreased
by
and HoengseongYakdak
meat extracts,
and
TNF- expressions
gene expressions
were not
by Hwangbong
changed
by chicken
and HoengseongYakdak
meat extracts. Conclusion:
breast Present
meat extracts,
study suggests
and TNF-that
gene
breast
expressions
meat of Korean
were not
native
changedmanifest
chicken
by chicken
its meat
anti- inflammatory
extracts.
effect, at least in part, by regulating the pro-inflammatory cytokine
Conclusion:
gene
expressions.
Present study suggests that breast meat of Korean native chicken manifest its anti-
Key words: korean native chicken, raw 264.7 cells, pro-inflammatory cytokine, gene expression, il-4, il-10
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-066
Evaluation of the use of fibre dietary in ice cream
M Boumba, Aniely; Castro, Yanires; Rodríguez, Tamara
Instituto de Investigación para la Industria Alimenticia, Cuba
[email protected]
It was evaluated the use of microcrystalline cellulose in the milk ice cream formulation by means of the
global evaluation of the product and its sensorial quality. For this purpose there were rehearsed different
proportions of the dietary fibre and in parallel was elaborated the control ice cream of milk. It was evaluat ed
the viscosity of the mixtures and the melting, yield and sensorial quality of the ice creams. To the selected
variant was evaluated the composition and general characteristics. With the level of 4.5 % of
microcrystalline cellulose was achieved a product of very good sensorial quality and satisfactory melting
and nutritional characteristics.
Key words: microcrystalline cellulose, ice cream
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-067 Development of fermented milks from buffalo´s semiskimmilk and culture of
lactobacillus acidophilus
M Boumba, Aniely; Iñiguez, Carola; Castro, Yanires
Instituto de Investigación para la Industria Alimenticia, Cuba
[email protected]
Pitching rate influence of industrial culture of Lactobacillus acidophilus on incubation time, acidity and pH
of fermented milk from buffalo ´s semi skim milk and 8 % of sugar, were studied. The best formula, with
inoculation rate and emulsion, was used for producing batches of fermented milk at pilot scale to conduct
physicochemical and microbiological analysis, and a sensory acceptance test. Shelf life was determined
trough the graphic method for incomplete failurer data using Weibull´s distribution. It was concluded that
the product showed good phisico - chemical, sensory and microbiologic characteristics. Shelf life at 4ºC
was 15 days during witch it maintains a cellular viabiity of was 10 8 UFC/g. In the mass acceptance test
this fermented milk rated within the "I like very much" and "I like extremaly intervals".
Key words: fermented milk, buffalo's semi skim milk, lactobacillus acidophilus
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-068
Soy ice cream for diabetics
Castro, Yanires; M Boumba, Aniely; Rodríguez, Tamara
Instituto de Investigación para la Industria Alimenticia, Cuba
[email protected]
The present work had as objective to obtain a frozen and
and functional dairy
dairy dessert, of
of appropriate
appropriate nutritional
nutritional
value,
that value,
benefits
that
thebenefits
consumers
the consumers
and especially
and to
especially
the populations
to the populations
clinically needy
clinically
as the
needy
diabetics.
as theIn this
diabetics.
ice
cream is present the soy because of its low glucémic index, their composition with a high level of dietary
In thislow-level
fiber,
ice cream
ofisfatpresent
and carbohydrates
the soy because
where
of its
thelow
sugar
glucémic
is not present.
index, their
There
composition
were carefully
with a selected
high levelthe
of dietary
raw
materials
fiber, to
low-level
use, keeping
of fat and
in carbohydrates
mind the intention
whereathe
developing
sugar is not
a low
present.
Calorie
There
icewere
cream.
carefully
Different
selected the raw
formulations
werematerials
evaluatedto where
use, keeping
sorbitolinwas
mind
present,
the intention
in order
a developing
to increase athe
lowtotal
Calorie
solids,
iceviscosity
cream. and
Different formulations
sweetness,
together fruit
were
pulps
evaluated
that imparted
where freshness
sorbitol was
andpresent,
good flavour.
in orderIt to
is concluded
increase the
that
total
is itsolids,
possible
viscosity
the
obtaining
and an
sweetness,
ice creamtogether
of satisfactory
fruit pulps
sensorial
that imparted
and nutritional
freshness
characteristic,
and good flavour.
with lowItcontain
is concluded
of fat and
that
a
caloric
is it possible
value 35%
thelower
obtaining
than an
theice
traditional
cream ofice
satisfactory
cream, for sensorial
feeding ofand
diabetics
nutritional
and characteristic,
other needful clinically
with
low contain
sectors.
In the
of fat
massive
and a caloric
acceptance
value 35%
evaluation
lower than
of the
theproduct,
traditional
80%
iceofcream,
the consumers
for feedingevaluated
of diabetics
theand
ice
other needful
sectors. In the massive acceptance evaluation of the product, 80% of the
cream
as I likeclinically
it very much.
consumers evaluated the ice cream as
I like it very much.
Key words: diabetics, soy milk, sorbitol, hipocaloric sweeters, ice cream
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-069
Use of cheese whey in ice cream
Castro, Yanires; M Boumba, Aniely; Rodríguez, Tamara; Seivanes, Ariel
Instituto de Investigación para la Industria Alimenticia, Cuba
[email protected]
It was evaluated the use of whey from cheddar cheese as substitute of non fatty solids of milk in ice cream
formulation, by means of the global evaluation of the product and its sensorial quality. Different substitution
levels (15, 25 and 35%) and ice cream control were elaborated at laboratory scale. The viscosity of de
mixtures, melting, yield and sensorial quality of the ice cream were evaluated. To the selected variant, the
composition and general characteristics were determined at pilot plant scale. With the lever 25% of
substitution of non fatty solids of milk for solids of whey, a product of very good sensorial quality, excellent
melting, yield, and satisfactory nutritional characteristics was achieved.
Key words: cheese whey, ice cream
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-070
Potentialities of leaves from Clusia minor L. species
Boffill Hernández, Guillermo; Mangas Marín, Raisa; Reynaldo Fernández, Gledys; Robeiro Guerra,
Idania; Bello Alarcón, Adonis
Centro Nacional de Investigaciones Científicas (CNIC), Cuba
[email protected]
Los estudios fitoquímicos realizados al género Clusia (Guttiferae) han demostrado que es una fuente rica
de
secundarios.
En estealestudio
propusimos
determinar
la capacidad
secuestradora
del
Losmetabolitos
estudios fitoquímicos
realizados
géneronos
Clusia
(Guttiferae)
han demostrado
que es
una fuente rica
de metabolitos
DPPH
y antiinflamatoria
secundarios.
de En
cuatro
este extractos
estudio nos
obtenidos
propusimos
a partir
determinar
de las lahojas
capacidad
de la secuestradora
especie endémica
del
ClusiaminorL.
Los extractos
se prepararon
maceración
en orden
polaridadendémica
(hexano (CMHDPPH y antiinflamatoria
de cuatro
extractospor
obtenidos
a partir
de lascreciente
hojas de de
la especie
A),
acetato de etilo
(CMH-B) ysemetanol
(CMH-C))
y por extracción
directa
con etanol
(CMH-E)).Los
estudios
ClusiaminorL.
Los extractos
prepararon
por maceración
en orden
creciente
de polaridad
(hexano
por
cromatografía
enetilo
capa(CMH-B)
delgaday revelaron
las diferencias
cualitativasdirecta
de estos
extractos.El
tamizaje
(CMH-A),
acetato de
metanol (CMH-C))
y por extracción
con etanol
(CMH-E)).Los
fitoquímicocorroboró
estas en
diferencias
cualitativas
se diferencias
detectaron cualitativas
principalmente
compuestos
como:
estudios por cromatografía
capa delgada
revelarony las
de estos
extractos.El
flavonoides,
triterpenos, compuestos
fenólicos.La
actividad ysecuestradora
radical, mediante
el ensayo
tamizaje fitoquímicocorroboró
estas diferencias
cualitativas
se detectarondel
principalmente
compuestos
de
DPPH,
mostró que
las fracciones
CMH-Bfenólicos.La
y CMH-E presentaron
un efecto significativo,
mientras
queella
como:
flavonoides,
triterpenos,
compuestos
actividad secuestradora
del radical,
mediante
actividad
en CMH-B
el modelo
de edema
en la oreja
ratónsignificativo,
inducido por aceite
ensayo deantiinflamatoria
DPPH, mostró de
queloslasextractos
fracciones
y CMH-E
presentaron
un de
efecto
de
crotónque
mostraron
un alto
por ciento de de
actividad
en todos
extractos
excepción
del extracto
mientras
la actividad
antiinflamatoria
los extractos
en los
el modelo
decon
edema
en la oreja
de ratónpolar
CMH-C.
inducido por aceite de crotón mostraron un alto por ciento de actividad en todos los extractos con
Key words: género clusia, actividad antioxidante, actividad antiinflamatoria, tamizaje fitoquímico,
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-072
Almonds consumption to reduce cholesterol and increase human health
Rodríguez Diego, Jesús G.; Villoch, Alejandra; Melo Ruíz, Virginia; Sánchez Herrera, Karina
Universidad Autónoma Metropolitana-Xochimilco, México
[email protected]; [email protected]
Almonds Intake as a tidbit snack, or
or as
as an
an ingredient
ingredient of
of numerous
numerous kind
kind of
ofgourmet
gourmet dishes
dishes either
either ininstews
stewsor
or desserts
in
in desserts
is aiscommon
a common
practice
practice
by by
population
population
all all
over
over
thethe
world,
world,
however,
however,
nutritional
nutritional
value
value
is not
is not
take
take
into
into account.
account.
Almonds
Almonds
are high
are high
in macronutrients
in macronutrients
mainly
mainly
in fatty
in fatty
acids,
acids,
not not
onlyonly
in monounsaturated
in monounsaturated
MUFA
MUFA
but
but also
also
in polyunsaturated
in polyunsaturated
PUFA
PUFA
fats,fats,
dietary
dietary
fiber,fiber,
and and
a variety
a variety
of phytonutrients
of phytonutrients
that that
believed
believed
to have
to have
cardio
cardio protective
protective
attributes.
attributes.
This study
This was
studyconducted
was conducted
to determine
to determine
the effects
the effects
of almonds
of almonds
intakeintake
on blood
on lipids
bloodtotal
and
lipidscholesterol.
and total cholesterol.
Observation
Observation
of 120 person's
of 120 person's
age of 50
agetoof65,
50 to
males
65, males
and females
and females
with with
normal
normal cholesterol
cholesterol
and hypercholesterolemia
and hypercholesterolemia
with consumption
with consumption
of 25 toof
125
25 g/day
to 125per
g/day
individual
per individual
during 18
during
weeks,
with
their own
diet.
Cholesterol
were assess
before
andbefore
after the
Total
cholesterol
18 weeks,
withregular
their own
regular
diet. Cholesterol
were
assess
andstudy.
after the
study.
Total presents
a
little reduction,
4.5%
andreduction,
5.5% respectively.
The result
of this The
study
show
a cardio
effect
cholesterol
presents
a little
4.5% and 5.5%
respectively.
result
of this
study protective
show a cardio
associated
with nut
consumption.
Cholesterol
levelCholesterol
is affected by
age,
sex and by
diet,
andsex
plays
significant
protective effect
associated
with nut
consumption.
level
is affected
age,
andadiet,
and
playsina different
role
significantmetabolism
role in different
functions
metabolism
in human
functions
health.in Almonds
human health.
consumption
Almonds lowers
consumption
total cholesterol;
lowers
total cholesterol;
however
more studies
however
regarding
more studies
this matter
regarding
should
this be
matter
performance,
should be taking
performance,
in consideration
taking in fatty acids
consideration
content
of almonds
fatty acids
and diet
content
and of
increase
almonds
theand
participant's
diet and increase
number the
in sample
participant's
for further
number
studies.
in sample
Almonds
for
should be incorporated as a regular habit in a daily diet, mainly in adults with cholesterol problems.
Key words: almonds consumption; to reduce cholesterol; human health
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-073 Enzymatic hydrolysis of whole bovine plasma protein: optimization and enzyme
activity of peptide fractions obtained by ultrafiltration
Figueroa Moreno, Omar Alfredo ; Zapata Montoya, José Edgar
Universidad Popular del César, Colombia
[email protected]
In this study, the
thereaction
reaction of
ofenzymatichydrolysis
enzymatic hydrolysisofofproteinsfromwholebovine
proteins from whole bovine
plasma
plasma
wasoptimized
was optimized
in ain a
batch reactor, using
usingthemethodology
the methodology of
of response
response surface (MSR), the
theantioxidantactivity
antioxidant activityof
of peptide fractions
peptidefractionswereanalyzedusinganalyticalmethodssuch
were
analyzed using analytical methods such as ABTS and as
FRAP
ABTS
and
and
system
FRAPultrafiltration
and
fractionation of
systemultrafiltrationfractionation
antioxidant
peptides interest was
of antioxidantpeptidesinterestwasdesigned.
designed. Alcalase 2.4L was used for Alcalase
the hydrolysis,
2.4L
where three
experimental
factors: pH,wherethree
temperatureexperimental
and enzyme-substrate
(E /S) ratio,and
were
analyzed in order
wasusedforthehydrolysis,
factors: pH, temperature
enzyme-substrate
(E / to
maximize
the degree ofinhydrolysis
(DH) reaction fort wohours
reaction.
GH was measured using the
S) ratio, wereanalyzed
order to maximizethedegree
of hydrolysis
(DH) The
reactionfortwohoursreaction.
method
of valuation of theproton (pH-stat).
The effect
of GH on(pH-stat).
experimental
factors
wasonadjusted to a
The GH wasmeasuredusingthemet
hod of valuation
of theproton
Theeffect
of GH
quadratic
equation
which has coefficient
of determination of has
0.931.
Optimalofreaction
conditions
for the
experimental
factorswasadjusted
to a quadraticequationwhich
coefficient
determination
of 0.931.
enzymatic
hydrolysis of bovine plasma were: Temperature
° C, were:
pH 9 Temperature
and E / S 10%
/ w).
Optimalreactionconditionsfortheenzymatichydrolysis
of bovine60.4
plasma
60.4(w° C,
pHThe
9
predicted
for/ GH
23%. Ananalysis of
experimental
factors evidences
the dual effect
and E / S value
10% (w
w). was
Thepredictedvaluefor
GHinteraction
was 23%.ofAnanalysis
of interaction
of experimental
factorsevidencesthe
of
temperature and pH,
dualon
effect
the other
of temperature
hand the and
direct
pH,
relationship
ontheotherhandthedirectrelationship
of the E / S in the assessed
of the
levels
E/S
was
in theassessedlevelswasnoted.
noted.
The antioxidant activity of
Theantioxidantactivity
bovine plasma enzymatic
of bovine
hydrolysates
plasma
with Alcalase 2.4 L, increases
enzymatichydrolysateswithAlcalase
significantly
with the GH. Through2.4anL, increasessignificantlywiththe
ultrafiltration system is possible
GH. is possible to concentrate
peptidefractions of molecular <8kDa weight, presenting significant antioxidant activityr elative to the native
protein and the complete hydrolyzate.
Key words:
ultrafiltration
Enzymatic hydrolysis, bovine plasma, protein, optimization, enzyme activity, peptide,
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-074
Probiotic fermented milk with prebiotic syrup
Rodríguez Martínez, Oxalis; Rodríguez Martínez, José Alberto; Trujillo, Luis E.; Pérez Cruz, Enrique R.;
Santos Carballal, Beatriz; Núñez de Villavicencio, Margarita
IIIA, Cuba
[email protected]
Among dairy products
products with
with functional
functional properties,
properties, the
thesymbiotic
symbioticfermented
fermentedmilks
milksare
arecommercially
commerciallyavaible
avaible
world wide. Numerous studies refer that the
the incorporation of probiotic
probiotic bacteria
bacteria and
and prebiotic
prebiotic product
product in
in the
the
for for
health.
fermented milk
milkare
arebeneficial
beneficial
health. Syrup prebiotic was used at 0.3 % and Bioyogur starter
Syrup
prebioticacidophilus
was used at+Streptococcus
0.3 % and Bioyogur
starter (Lactobacillus
acidophilus
+Streptococcus
(Lactobacillus
thermophilus)
was inoculated
at 2 % v/v.
Lactic acid formation
thermophilus)
at for
2 %samples
v/v. Lactic
acid formation
and phinoculated
values were
determined
and
ph values was
wereinoculated
determined
collected
at the recently
milk
and every for
0.5 h to the
samples collected
at thecontents
recently at
inoculated
milkcellular
and every
0.5 hoftoL.the
coagulation.
The
sugardetermined.
contents
coagulation.
The sugar
0 y 3 h and
viability
acidophilus
at 24
h were
at 0 yglobal
3 h and
cellular
of L.The
acidophilus
24 h were
determined.plastics
The global
qualityand
wasstored at 4 The
quality
wasviability
evaluated.
shelf life at
parked
in polyethylene
containers
evaluated.
6
oC was The
determined.
shelf life The
parked
acidification
in polyethylene
capacity
plastics
and containers
growth of and
starters
stored
were
at 4not
- 6 affected
oC was of the used
determined.
prebiotic
syrup. The syrup for growth meanwhile the fructooligosaccharides fraction was intact. The
The acidification
products
had good
capacity
sensorial,
and physics-chemestry
growth of starters were
and not
microbiological
affected of the
quality.
usedThe
prebiotic
viability
syrup.
of L. The
acidophilus
syrup
for growthcounts
reached
meanwhile
in order
theoffructooligosaccharides
1010 ufc/mL. The shelffraction
life waswas
21 days.
intact. The products had good sensorial,
physics-chemestry and microbiological quality. The viability of L. acidophilus reached counts in order of
Key words: probiotic, prebiotic, fermented milk, fructooligosaccharides, symbiotic
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-075
Mangiferin solubility in different solvents used in the pharmaceutical industry
Sevilla Fernández, Iliana
Universidad de La Habana (UH), Cuba
[email protected]
Mangiferin an important bioactive constituent of mango with numerous pharmacological properties. The
Mangiferin
an important
bioactive in
constituent
mango
with numerous
pharmacological
properties. The
solubility behavior
is important
order to of
obtain
complete
information
about physicochemical
data.
solubility
behavior
is
important
in
order
to
obtain
complete
information
about
physicochemical
Otherwise, temperature-solubility dependence allows perform the respective thermodynamicdata.
analysis. In
Otherwise,
dependence
allows perform
the respective
In
the
present temperature-solubility
work, we have measurement
the solubility
of mangiferin
in waterthermodynamic
and six organic analysis.
compounds
the present used
work,inwe
measurementindustry
the solubility
mangiferin
and
six organic Materials
compounds
commonly
thehave
pharmaceutical
in the of
range
of 5 toin60water
0C of
temperature.
and
commonly
usedmass
in theofpharmaceutical
of 5 in
to the
60 0C
of temperature.
Materials
andin a
methods: The
mangiferin was industry
chosen in
tothe
be range
in excess
solvents.
This solution
was put
methods:
The
mass
of
mangiferin
was
chosen
to
be
in
excess
in
the
solvents.
This
solution
was
put
in a
glass vessel inside an incubator under constant agitation for about 3 h at work temperature. The solution
glass
vessel
inside
an
incubator
under
constant
agitation
for
about
3
h
at
work
temperature.
The
solution
was centrifuged and the samples were filtered through a 0.45 µm membrane. Concentrations were
was centrifuged
and the samples
were in
filtered
a 0.45 µm membrane.
Concentrations
were
determined
by measuring
absorbance
a UV through
spectrophotometer
at 254 nm. Results
and discussion:
The
determined
measuringincreased
absorbance
a UV
spectrophotometer
at 254 nm.
and discussion:
solubility
of by
mangiferin
withinan
increased
in the temperature
in Results
each solvent.
MangiferinThe
is
solubility
mangiferin
increased
with
an increased
in theand
temperature
each solvent.
Mangiferin
is ether,
sparingly of
soluble
in ethanol,
slightly
soluble
in methanol
water and in
practically
insoluble
in diethyl
sparingly
soluble
in ethanol,
slightly soluble
in methanol
and water
andVan?
practically
diethyl
ether,
acetone and
n hexane.
The solubility
were correlated
according
to the
t Hoff insoluble
equation,inthe
standards
acetone
and
n
hexane.
The
solubility
were
correlated
according
to
the
Van?
t
Hoff
equation,
the
heat of solution was obtained from the slope of ln Cs vs 1/T plot. Conclusions: Solubility of mangiferin was
standards heat
of solution was
obtained
the slope
of ln Cs vs 1/T
Conclusions:
Solubility
of
determined
experimentally
in six
solventsfrom
at different
temperatures.
Forplot.
all cases
was observed
increased
mangiferin
was determined
experimentally
in six solvents
at differentThe
temperatures.
Forsolubility
all casesdata
was were
in the solubility
of mangiferin
respect to increase
of temperature.
experimental
observed
the equation
solubility and
of mangiferin
respect
to increase
of for
temperature.
The experimental
correlated increased
with Van´tinHoff
the standards
heats
of solution
the
solubility
data
were
correlated
with
Van´t
Hoff
equation
and
the
standards
heats
of
solution
for the
different solvents were calculated.
Key words: mangiferin solubility, solvents, pharmaceutical industry
16th International Scientific Congress CNIC'2015
National Center for Scientific Research
Address:
Ave 25 y 158, Cuba nacán, Playa. AP 6414, La Ha bana, Cuba.
www: http://www.cnic.edu.cu/
50 th Anniversary, 1965 ‐ 2015
SPN-P-076 Assessment of genotoxic and cytotoxic effectsof Phyllanthus plants aqueous extracts
in Caulobacter crescentus
Menéndez Perdomo, Ivette María ; Sánchez Lamar, Ángel
Universidad de La Habana (UH), Cuba
[email protected]
Plants of the genus Phyllanthus have
have been
been widely
widely used
used by
by traditional
traditional medicineall
medicineall over
over the
theworld.
world.InInCuba,
Cuba,
there is aa plentiful
plentiful variety
variety and
andendemism
endemismofofthem,
them,and
andvarious
variousspecies
species
have
haveproven
provenantiviral,
antiviral,
antioxidant
antioxidant
and antimutagenic properties against
against chemical
chemicaland
and physical
physicalmutagens.
mutagens.InInorder
ordertotoextend
extendthe
thesearch
searchforfor
natural compounds
compounds presentinggenoprot
presentinggenoprotective
ective properties
propertiesto to
other
other
species
species
of this
of genus
this genus
growngrown
in our in our
country, previous
previous studies
studies about
abouttheir
theirpossible
possiblegenotoxic
genotoxicand
and
cytotoxic
cytotoxic
properties
properties
must
must
be done.
be done.
In the
In the
present work,
work, the
theaqueous
aqueousextracts
extracts
of four
of four
Phyllanthus
Phyllanthus
species
species
were were
assessed:
assessed:
P. williamioides,
P. williamioides,
P.
P.
chamaecristoides,P.
chamaecristoides,
P.microdictyus,
microdictyus,and
andP.P.epiphyllanthus,
epiphyllanthus,atatconcentrations
concentrationsranging
rangingfrom
from
0.10.1
to 2
tomg/ml
2 mg/ml
and using
using Caulobactercrescentus
Caulobactercrescentus cells
cellsasasthethe
in vitroexperimental
in vitroexperimental
model.Genotoxicity
model. Genotoxicity
was assessed
was assessed
through evaluating
evaluating primary
primaryDNA
DNAdamage
damage
by by
means
means
of the
of SOS
the SOS
Colorimetric
Colorimetric
assay.assay.
Cytotoxicity
Cytotoxicity
was was
measured throughcolony
throughcolony formation
formation assay.
assay.AsAs
a result,
a result,
the the
genotoxic
genotoxic
and cytotoxic
and cytotoxic
effectseffects
were only
were only
statistically significant by
by way
way of
of aaDunnett
DunnettTest
Test(p<0.05),
(p<0.05),for
forthe
thehighest
highestconcentration
concentrationtested
testedand
andonly
onlyin in
In In
all all
cases
the the
LD50values
werewere
higher
than than
the maximum
case of
of the
theP.
P.williamioides
williamioidesplant
plantextract.
extract.
cases
LD50values
higher
the maximum
concentration assayed and
and P.
P.chamaecristoidesandP.
chamaecristoidesandP. microdictyus
microdictyusextracts
extractsshowed
showeda asurvival
survivalrate
rateover
over
are in
in correspondence
correspondence with
withpreceding
preceding studies
studiesabout
aboutthe
thegenotoxicity
genotoxicityand
andcytotoxicity
90%. These outcomes are
cytotoxicity
of several Phyllanthus
species,
withexperimental
different experimental
vitro
andand
in vivo,
of
several Phyllanthus
species, with
different
models, inmodels,
vitro andin in
vivo,
they and
validat e
future
researches
the possible
properties of these
plants
extracts.
they validate
futureabout
researches
about antimutagenic
the possible antimutagenic
properties
of aqueous
these plants
aqueous
Key words: phyllanthus, caulobactercrescentus,genotoxicity, cytotoxicity, plant aqueous extract